protein denaturation

Summary

Top Publications

  1. ncbi IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
    Marcella Calfon
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Nature 415:92-6. 2002
  2. ncbi Intrinsically disordered proteins in human diseases: introducing the D2 concept
    Vladimir N Uversky
    Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Annu Rev Biophys 37:215-46. 2008
  3. ncbi Protein disaggregation mediated by heat-shock protein Hsp104
    D A Parsell
    Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, Illinois 60637
    Nature 372:475-8. 1994
  4. ncbi Regulated translation initiation controls stress-induced gene expression in mammalian cells
    H P Harding
    Skirball Institute of Biomolecular Medicine The Department of Medicine, Kaplan Cancer Center New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 6:1099-108. 2000
  5. pmc Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha
    I Novoa
    Skirball Institute of Biomolecular Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 153:1011-22. 2001
  6. ncbi Protein kinetic stability
    Jose M Sanchez-Ruiz
    Departamento de Quimica Fisica, Universidad de Granada, 18071 Granada, Spain
    Biophys Chem 148:1-15. 2010
  7. ncbi Conformational constraints for amyloid fibrillation: the importance of being unfolded
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1698:131-53. 2004
  8. pmc Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, ON, Canada M5G 1L5
    Proc Natl Acad Sci U S A 103:15835-40. 2006
  9. pmc Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form
    Maria J Serrano-Vega
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 0QH, United Kingdom
    Proc Natl Acad Sci U S A 105:877-82. 2008
  10. ncbi Protein stability and surface electrostatics: a charged relationship
    Samantha S Strickler
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 45:2761-6. 2006

Detail Information

Publications317 found, 100 shown here

  1. ncbi IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA
    Marcella Calfon
    Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA
    Nature 415:92-6. 2002
    ..Our findings suggest that physiological ER load regulates a developmental decision in higher eukaryotes...
  2. ncbi Intrinsically disordered proteins in human diseases: introducing the D2 concept
    Vladimir N Uversky
    Center for Computational Biology and Bioinformatics, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Annu Rev Biophys 37:215-46. 2008
    ..From these and other examples, novel strategies for drug discovery based on IDPs have been developed. To summarize work in this area, we are introducing the D2 (disorder in disorders) concept...
  3. ncbi Protein disaggregation mediated by heat-shock protein Hsp104
    D A Parsell
    Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, Illinois 60637
    Nature 372:475-8. 1994
    ..Rather, Hsp104 functions in a manner not previously described for other heat-shock proteins: it mediates the resolubilization of heat-inactivated luciferase from insoluble aggregates...
  4. ncbi Regulated translation initiation controls stress-induced gene expression in mammalian cells
    H P Harding
    Skirball Institute of Biomolecular Medicine The Department of Medicine, Kaplan Cancer Center New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 6:1099-108. 2000
    ..Mammalian cells thus utilize an ancient pathway to regulate gene expression in response to diverse stress signals...
  5. pmc Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha
    I Novoa
    Skirball Institute of Biomolecular Medicine, Department of Medicine, New York University School of Medicine, New York, NY 10016, USA
    J Cell Biol 153:1011-22. 2001
    ....
  6. ncbi Protein kinetic stability
    Jose M Sanchez-Ruiz
    Departamento de Quimica Fisica, Universidad de Granada, 18071 Granada, Spain
    Biophys Chem 148:1-15. 2010
    ....
  7. ncbi Conformational constraints for amyloid fibrillation: the importance of being unfolded
    Vladimir N Uversky
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Biochim Biophys Acta 1698:131-53. 2004
    ..The inherent flexibility of such an intermediate is essential in allowing the conformational rearrangements necessary to form the core cross-beta structure of the amyloid fibril...
  8. pmc Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination
    Masoud Vedadi
    Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, ON, Canada M5G 1L5
    Proc Natl Acad Sci U S A 103:15835-40. 2006
    ..The methods are cost-effective, can be implemented in any laboratory, promise to increase the success rates of purifying and crystallizing human proteins significantly, and identify new ligands for these proteins...
  9. pmc Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form
    Maria J Serrano-Vega
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 0QH, United Kingdom
    Proc Natl Acad Sci U S A 105:877-82. 2008
    ..In addition, betaAR-m23 was significantly more stable in a wide range of detergents ideal for crystallization and was preferentially in an antagonist conformation in the absence of ligand...
  10. ncbi Protein stability and surface electrostatics: a charged relationship
    Samantha S Strickler
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Biochemistry 45:2761-6. 2006
    ....
  11. ncbi Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery
    Mei Chu Lo
    Biophysics Enzymology Chemical and Screening Sciences, Wyeth Research, Pearl River, NY 10965, USA
    Anal Biochem 332:153-9. 2004
    ..The potential pitfalls in the data analysis of thermal shift assays are also discussed...
  12. ncbi Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism
    L Nielsen
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 40:6036-46. 2001
    ..A kinetic model, involving the association of monomeric partially folded intermediates, whose concentration is stimulated by the air-water interface, leading to formation of the critical nucleus and thence fibrils, is proposed...
  13. ncbi Protein folding and misfolding
    Christopher M Dobson
    University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge CB2 1EW, UK
    Nature 426:884-90. 2003
    ..Aggregation of misfolded proteins that escape the cellular quality-control mechanisms is a common feature of a wide range of highly debilitating and increasingly prevalent diseases...
  14. ncbi The mammalian unfolded protein response
    Martin Schroder
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom
    Annu Rev Biochem 74:739-89. 2005
    ..Finally, we summarize data that demonstrate that UPR signaling feeds into decision making in other processes previously thought to be unrelated to ER function, e.g., eukaryotic starvation responses and differentiation programs...
  15. ncbi A regulatory link between ER-associated protein degradation and the unfolded-protein response
    R Friedlander
    Max Delbruck Centrum fur Molekulare Medizin, Berlin, Germany
    Nat Cell Biol 2:379-84. 2000
    ..However, when stress is applied, the UPR is required to increase ERAD activity. We thus demonstrate, for the first time, a regulatory loop between ERAD and the UPR, which is essential for normal growth of yeast cells...
  16. pmc Strain-specified relative conformational stability of the scrapie prion protein
    D Peretz
    Institute for Neurodegenerative Diseases, University of California, San Francisco, California 94143, USA
    Protein Sci 10:854-63. 2001
    ..Based on these results, the eight prion strains segregated into four distinct groups. Our results support the unorthodox proposal that distinct PrP(Sc) conformers encipher the biological properties of prion strains...
  17. pmc Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB
    A Mogk
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, 79104 D Freiburg, Germany
    EMBO J 18:6934-49. 1999
    ....
  18. ncbi Effects of common cancer mutations on stability and DNA binding of full-length p53 compared with isolated core domains
    Hwee Ching Ang
    Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 2QH, United Kingdom
    J Biol Chem 281:21934-41. 2006
    ..Many mutants that have lowered melting temperatures should be good drug targets, although the common R273H mutant binds response elements too weakly for simple rescue...
  19. ncbi Evidence for an unfolding/threading mechanism for protein disaggregation by Saccharomyces cerevisiae Hsp104
    Ronnie Lum
    Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 279:29139-46. 2004
    ....
  20. ncbi Therapeutic approaches to protein-misfolding diseases
    Fred E Cohen
    University of California at San Francisco, Department of Cellular and Molecular Pharmacology, Genentech Hall, 600 16th Street N472J, San Francisco, California 94107, USA
    Nature 426:905-9. 2003
    ..Molecules are now emerging that link our biophysical insights with our therapeutic aspirations...
  21. pmc Role of solvation effects in protein denaturation: from thermodynamics to single molecules and back
    Jeremy L England
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA
    Annu Rev Phys Chem 62:257-77. 2011
    ..Finally, we present recent single-molecule fluorescence studies of denatured proteins, the analysis of which corroborates the role of denaturants in shifting the equilibrium of the coil-globule transition...
  22. ncbi Amyloid-like properties of bacterial inclusion bodies
    Mar Carrió
    Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 08193 Bellaterra Barcelona, Spain
    J Mol Biol 347:1025-37. 2005
    ....
  23. pmc Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network
    P Goloubinoff
    Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Proc Natl Acad Sci U S A 96:13732-7. 1999
    ....
  24. ncbi Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
    Benjamin Schuler
    Laboratory of Chemical Physics, Building 5, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Nature 419:743-7. 2002
    ..Unlike the activation energy, the free-energy barrier includes the activation entropy and thus has been elusive to experimental determination for any kinetic process in solution...
  25. ncbi Sequence complexity of disordered protein
    P Romero
    School of Electrical Engineering and Computer Science, Washington State University, Pullman, Washington 99164 4660, USA
    Proteins 42:38-48. 2001
    ....
  26. ncbi Hsp104, Hsp70, and Hsp40: a novel chaperone system that rescues previously aggregated proteins
    J R Glover
    Howard Hughes Medical Institute and Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Cell 94:73-82. 1998
    ..coli. We conclude that Hsp104 has a protein remodeling activity that acts on trapped, aggregated proteins and requires specific interactions with conventional chaperones to promote refolding of the intermediates it produces...
  27. ncbi Correlation of levels of folded recombinant p53 in escherichia coli with thermodynamic stability in vitro
    Sebastian Mayer
    Cambridge University Chemical Laboratory and Cambridge Centre for Protein Engineering, MRC Centre, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 372:268-76. 2007
    ..coli, and provide insights into the correlation between protein instability and disease at the cellular level...
  28. pmc Distinct roles of activating transcription factor 6 (ATF6) and double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (PERK) in transcription during the mammalian unfolded protein response
    Tetsuya Okada
    Graduate School of Biostudies, Kyoto University, 46 29 Yoshida Shimoadachi, Sakyo ku, Kyoto 606 8304, Japan
    Biochem J 366:585-94. 2002
    ..Thus we propose that mammalian cells have evolved a strategy to cope with ER stress different from that of yeast cells...
  29. ncbi Why is trehalose an exceptional protein stabilizer? An analysis of the thermal stability of proteins in the presence of the compatible osmolyte trehalose
    Jai K Kaushik
    Centre for Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India
    J Biol Chem 278:26458-65. 2003
    ..8 kcal mol-1 at pH 2.5. There is a decrease in the heat capacity of protein denaturation (DeltaCp) in trehalose solutions for all the studied proteins...
  30. ncbi Unfolding of proteins and long transient conformations detected by single nanopore recording
    G Oukhaled
    Laboratoire de Recherche sur les Polymeres, Equipe Matériaux Polymères aux Interfaces, CNRS UMR 7581, Université d Evry, 91025 Evry, France
    Phys Rev Lett 98:158101. 2007
    ..Long blockades reveal partially folded conformations. Their duration increases as the proteins are more folded. The observation of a Vogel-Fulcher law suggests a glassy behavior...
  31. pmc In vitro unfolding, refolding, and polymerization of human gammaD crystallin, a protein involved in cataract formation
    Melissa S Kosinski-Collins
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Protein Sci 12:480-90. 2003
    ..The mechanism of HgammaD-Crys aggregation may provide clues to understanding age-onset cataract formation in vivo...
  32. ncbi Thermal stability of alpha-amylase in aqueous cosolvent systems
    Jay Kant Yadav
    Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore 570020, India
    J Biosci 34:377-87. 2009
    ..Preferential interaction parameters indicated extensive hydration of the enzyme in the presence of cosolvents...
  33. pmc Heat shock protein coinducers with no effect on protein denaturation specifically modulate the membrane lipid phase
    Zsolt Torok
    Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, P O Box 521, H 6701 Szeged, Hungary
    Proc Natl Acad Sci U S A 100:3131-6. 2003
    ..We show here that the presence of BM does not influence protein denaturation in the cells...
  34. ncbi Immunolocalisation of PrPSc in scrapie-infected N2a mouse neuroblastoma cells by light and electron microscopy
    Nathalie M Veith
    Molecular Toxicology, Department of Biology, University of Konstanz, Box X911, D 78457 Konstanz, Germany
    Eur J Cell Biol 88:45-63. 2009
    ..Finally, we observed the release of PrPC/PrPSc via exocytotic pathways, i.e. via exosomes and as an opaque electron-dense mass which may represent a mechanism of intercellular spreading of infectious prions...
  35. ncbi A hydrogen bond surrogate approach for stabilization of short peptide sequences in alpha-helical conformation
    Anupam Patgiri
    Department of Chemistry, New York University, New York, New York 10003, USA
    Acc Chem Res 41:1289-300. 2008
    ..Notably, our preliminary studies illustrate that HBS helices can target their expected protein receptors with high affinity...
  36. ncbi Interaction of urea with amino acids: implications for urea-induced protein denaturation
    Martin C Stumpe
    Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    J Am Chem Soc 129:16126-31. 2007
    The molecular mechanism of urea-induced protein denaturation is not yet fully understood...
  37. pmc A functional single-molecule binding assay via force spectroscopy
    Yi Cao
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada V6T 1Z1
    Proc Natl Acad Sci U S A 104:15677-81. 2007
    ..This methodology opens up avenues for studying protein-ligand interactions in a functional context, and we anticipate that it will find broad application in diverse protein-ligand systems...
  38. pmc Atypical AAA+ subunit packing creates an expanded cavity for disaggregation by the protein-remodeling factor Hsp104
    Petra Wendler
    Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, UK
    Cell 131:1366-77. 2007
    ..The large cavity could enable the uptake of polypeptide loops without a requirement for exposed N or C termini...
  39. pmc Thermal adaptation of viruses and bacteria
    Peiqiu Chen
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts Department of Physics, Harvard University, Cambridge, Massachusetts, USA
    Biophys J 98:1109-18. 2010
    ....
  40. ncbi More stable structure of wheat germ lipase at low pH than its native state
    Ejaz Ahmad
    Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India
    Biochimie 92:885-93. 2010
    ..Although enzymatically inactive, acid-induced state at pH 0.8 was found to be structurally more stable than native lipase, as shown by chemical and thermal denaturation profiles...
  41. pmc First-order rate-determining aggregation mechanism of p53 and its implications
    Guozhen Wang
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Proc Natl Acad Sci U S A 109:13590-5. 2012
    ....
  42. ncbi Rapid and selective characterization of influenza virus constituents in monovalent and multivalent preparations using non-porous reversed-phase high performance liquid chromatography columns
    Virginia García-Cañas
    Centre for Biologics Research, Health Canada, Banting Bldg, Tunney s Pasture, Ottawa, Ont, Canada K1A 0L2
    J Chromatogr A 1123:225-32. 2006
    ..Several influenza constituents were detected including nucleoprotein, the highly hydrophobic matrix protein and the primary surface antigen, haemagglutinin (HA)...
  43. pmc Tracking mutant huntingtin aggregation kinetics in cells reveals three major populations that include an invariant oligomer pool
    Maya A Olshina
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, Victoria 3010, Australia
    J Biol Chem 285:21807-16. 2010
    ....
  44. pmc The cold denatured state of the C-terminal domain of protein L9 is compact and contains both native and non-native structure
    Bing Shan
    Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794 3400, USA
    J Am Chem Soc 132:4669-77. 2010
    ..8 unfolded state of wild type CTL9 at 25 degrees C, suggesting that it is a robust feature of the denatured state ensemble of this protein. The implications for protein folding and for studies of cold denatured states are discussed...
  45. ncbi Understanding cold denaturation: the case study of Yfh1
    Miquel Adrover
    MRC National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom
    J Am Chem Soc 132:16240-6. 2010
    ..We discuss the implications of our findings for understanding cold denatured states...
  46. ncbi Microscale fluorescent thermal stability assay for membrane proteins
    Alexander I Alexandrov
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Structure 16:351-9. 2008
    ..To evaluate the method, three different protein families were analyzed, including the Apelin G protein-coupled receptor (APJ)...
  47. pmc Structure and folding of a designed knotted protein
    Neil P King
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095 1569, USA
    Proc Natl Acad Sci U S A 107:20732-7. 2010
    ..The success of the design strategy--connecting two monomers of an intertwined homodimer into a single protein chain--supports a model for evolution of knotted structures via gene duplication...
  48. ncbi Protein folding and unfolding at atomic resolution
    Alan R Fersht
    Department of Chemistry, MRC Centre for Protein Engineering, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Cell 108:573-82. 2002
    ..We are on the threshold of predicting those protein folding events using simulation that has been carefully benchmarked by experiment...
  49. ncbi Electrostatic interactions across the dimer-dimer interface contribute to the pH-dependent stability of a tetrameric malate dehydrogenase
    Alexandra Bjørk
    Department of Biology, University of Oslo, Box 1031 Blindern, N 0316 Oslo, Norway
    FEBS Lett 553:423-6. 2003
    ..8 degrees C, and depended strongly on pH. Gel-filtration experiments indicated that the 26.8 degrees C loss in stability observed for the D56K mutant at low pH was accompanied by a shift towards a lower oligomerization state...
  50. pmc Amyloid fibril formation can proceed from different conformations of a partially unfolded protein
    Martino Calamai
    Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom
    Biophys J 89:4201-10. 2005
    ..In addition, the structures of the resulting aggregates are largely independent of the conformational properties of their soluble precursors...
  51. pmc Thermally induced fibrillar aggregation of hen egg white lysozyme
    Luben N Arnaudov
    Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Wageningen, The Netherlands
    Biophys J 88:515-26. 2005
    ..These fibrils have a coiled structure with a periodicity of approximately 30 nm and show characteristic defects after every four or five turns...
  52. pmc A network representation of protein structures: implications for protein stability
    K V Brinda
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560012, India
    Biophys J 89:4159-70. 2005
    ..Based on these results, we also predict a few residues in the thermophilic and mesophilic proteins that can be mutated to alter their thermal stability...
  53. ncbi Comparative studies on the structure and stability of fluorescent proteins EGFP, zFP506, mRFP1, "dimer2", and DsRed1
    Olesia V Stepanenko
    Institute of Cytology, Russian Academy of Sciences, St Petersburg 194064, Russia
    Biochemistry 43:14913-23. 2004
    ..This means that the quaternary structure, being an important stabilizing factor, does not represent the only circumstance dictating the dramatic variations between fluorescent proteins in their conformational stabilities...
  54. ncbi Protein aggregation in crowded environments
    R John Ellis
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Biol Chem 387:485-97. 2006
    ..This review discusses the quantitative effects of crowding on protein aggregation and the role of molecular chaperones in combating this problem...
  55. ncbi Fibrillar aggregates of the tumor suppressor p53 core domain
    Daniella Ishimaru
    Departamento de Bioquímica Médica and Centro Nacional de Ressonância Magnética Nuclear, Universidade Federal do Rio de Janeiro, 21941 590 Rio de Janeiro, RJ, Brazil
    Biochemistry 42:9022-7. 2003
    ..Fibrillar aggregates of p53C contribute to the loss of function of p53 and seed the accumulation of conformationally altered protein in some cancerous cells...
  56. pmc Probing structural heterogeneities and fluctuations of nucleic acids and denatured proteins
    Ted A Laurence
    Physical Biosciences Institute, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
    Proc Natl Acad Sci U S A 102:17348-53. 2005
    ..We propose that the larger fluctuations may indicate transient residual structure in the unfolded state...
  57. pmc Random-coil behavior and the dimensions of chemically unfolded proteins
    Jonathan E Kohn
    Interdepartmental Program in Biomolecular Science and Engineering, University of California Santa Barbara, Santa Barbara, CA 93106, USA
    Proc Natl Acad Sci U S A 101:12491-6. 2004
    ..588 predicted for an excluded volume random coil. Therefore, it appears that the mean dimensions of the large majority of chemically denatured proteins are effectively indistinguishable from the mean dimensions of a random-coil ensemble...
  58. ncbi The unfolded protein response is involved in the pathology of Alzheimer's disease
    Takashi Kudo
    Department of Psychiatry and Behavioral Science, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Ann N Y Acad Sci 977:349-55. 2002
    ..Experimental manipulation of IRE1, PERK, or eIF2alpha phosphorylation or GRP78 expression might allow the development of therapeutic strategies for FAD...
  59. ncbi Identifying unfolding intermediates of FN-III(10) by steered molecular dynamics
    Mu Gao
    Department of Physics and Beckman Institute, University of Illinois, Urbana 61801, USA
    J Mol Biol 323:939-50. 2002
    ..The simulations revealed three forced unfolding pathways of FN-III(10), one of which is preferentially selected under physiological conditions. Implications for fibronectin fibrillogenesis are discussed...
  60. ncbi Protease-sensitive scrapie prion protein in aggregates of heterogeneous sizes
    Salit Tzaban
    Department of Molecular Biology, The Hebrew University Hadassah Medical School, and Hadassah University Hospital, Jerusalem 91120, Israel
    Biochemistry 41:12868-75. 2002
    ..Whether these new PrP(Sc) species play a role in the biogenesis or the pathogenesis of prions remains to be established...
  61. ncbi Increasing temperature accelerates protein unfolding without changing the pathway of unfolding
    Ryan Day
    Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195 7610, USA
    J Mol Biol 322:189-203. 2002
    ..We conclude that 498K simulations are suitable for elucidating the details of protein unfolding at a minimum of computational expense...
  62. ncbi Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins
    Stella Chun
    Centre for Biologics Research, Biologics and Genetic Therapies Directorate, HPFB, Health Canada, Ottawa, Ontario, Canada
    Vaccine 26:6068-76. 2008
    ..To our knowledge, this is the first report on the quantitative determination of virtually all influenza vaccines using a single universal antibody...
  63. ncbi Ageing and vision: structure, stability and function of lens crystallins
    Hans Bloemendal
    Department of Biochemistry, University of Nijmegen, 6500HB, The Netherlands
    Prog Biophys Mol Biol 86:407-85. 2004
    ..Understanding the structural basis of protein stability and interactions in the healthy eye lens is the route to solve the enormous medical and economical problem of cataract...
  64. ncbi Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol
    T Tomoyasu
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
    Mol Microbiol 40:397-413. 2001
    ..At 42 degrees C, ClpXP and Lon became essential for viability of cells with low DnaK levels, indicating synergistic action of proteases and the DnaK system, which is essential for cell growth at 42 degrees C...
  65. ncbi Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines
    Andreas Martin
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 437:1115-20. 2005
    ..Energy-dependent machines with highly diverse quaternary architectures and molecular functions could operate by similar asymmetric mechanisms...
  66. ncbi Stabilization of an immunoglobulin fold domain by an engineered disulfide bond at the buried hydrophobic region
    Yoshihisa Hagihara
    Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology AIST, Ikeda, Osaka 563 8577, Japan
    J Biol Chem 282:36489-95. 2007
    ..The introduction of a hydrophobic cystine into the hydrophobic region maintains the hydrophobicity of the protein and is expected to minimize the unfavorable mutational effects...
  67. pmc The density and refractive index of adsorbing protein layers
    Janos Voros
    BioInterface Group, Laboratory for Surface Science and Technology, ETH Zurich, Schlieren, Switzerland
    Biophys J 87:553-61. 2004
    ..Our measurements indicated that water and solvent molecules not only influence the 3D structure of proteins in solution but also play a crucial role in their adsorption onto surfaces...
  68. ncbi Parkin suppresses unfolded protein stress-induced cell death through its E3 ubiquitin-protein ligase activity
    Y Imai
    Laboratory for Motor System Neurodegeneration, RIKEN Brain Science Institute BSI, Saitama 351 0198, Japan
    J Biol Chem 275:35661-4. 2000
    ....
  69. ncbi Impact of the deltaF508 mutation in first nucleotide-binding domain of human cystic fibrosis transmembrane conductance regulator on domain folding and structure
    Hal A Lewis
    Structural GenomiX, Inc, San Diego, California 92121, USA
    J Biol Chem 280:1346-53. 2005
    ....
  70. pmc Invariant amino acids essential for decoding function of polypeptide release factor eRF1
    Petr Kolosov
    Engelhardt Institute of Molecular Biology, The Russian Academy of Sciences, 119991 Moscow, Russia
    Nucleic Acids Res 33:6418-25. 2005
    ..The data point to a pivotal role played by the YxCxxxF motif (positions 125-131) in purine discrimination of the stop codons. We speculate that eRF1 decoding site is formed by a 3D network of amino acids side chains...
  71. pmc A backbone-based theory of protein folding
    George D Rose
    T C Jenkins Department of Biophysics, The Johns Hopkins University, Jenkins Hall, 3400 North Charles Street, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 103:16623-33. 2006
    ..Then, under folding conditions, the resultant fold is selected from a limited repertoire of structural possibilities, each corresponding to a distinct hydrogen-bonded arrangement of alpha-helices and/or strands of beta-sheet...
  72. pmc Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli
    Martin Hammarström
    Department of Biotechnology, Royal Institute of Technology KTH Center for Physics, Astronomy and Biotechnology, S 106 91 Stockholm, Sweden
    Protein Sci 11:313-21. 2002
    ..coli...
  73. ncbi Structural basis of protein kinetic stability: resistance to sodium dodecyl sulfate suggests a central role for rigidity and a bias toward beta-sheet structure
    Marta Manning
    Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 110 8th Street, Troy, New York 12180, USA
    Biochemistry 43:11248-54. 2004
    ..Remarkably, most of the kinetically stable SDS-resistant proteins in this study are oligomeric beta-sheet proteins, suggesting a bias of these types of structures toward kinetic stability...
  74. ncbi Eight prion strains have PrP(Sc) molecules with different conformations
    J Safar
    Department of Neurology, University of California, San Francisco 94143 0518, USA
    Nat Med 4:1157-65. 1998
    ....
  75. pmc Type I collagen is thermally unstable at body temperature
    E Leikina
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:1314-8. 2002
    ..Apparently, Nature adjusts collagen hydroxyproline content to ensure that the melting temperature of triple helical monomers is several degrees below rather than above body temperature...
  76. ncbi Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: a fluorescence spectroscopic analysis
    N A Bushmarina
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia
    Chembiochem 2:813-21. 2001
    ....
  77. ncbi Rationalization of the effects of mutations on peptide and protein aggregation rates
    Fabrizio Chiti
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK
    Nature 424:805-8. 2003
    ....
  78. pmc BPPred: a Web-based computational tool for predicting biophysical parameters of proteins
    Christian D Geierhaas
    Department of Chemistry, University of Cambridge, UK
    Protein Sci 16:125-34. 2007
    ..Taken together, these results suggest that BPPred should represent a valuable tool for interpreting experimental measurements, as well as the results of molecular dynamics simulations...
  79. pmc Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism
    Norma J Greenfield
    Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, New Jersey 08854 8021, USA
    Nat Protoc 1:2733-41. 2006
    ..The experiments require 2-5 h...
  80. pmc Predicting the energetics of osmolyte-induced protein folding/unfolding
    Matthew Auton
    Department of Human Biological Chemistry, University of Texas Medical Branch, 301 University Boulevard, 5 154 Medical Research Building, Galveston, TX 77555 1052, USA
    Proc Natl Acad Sci U S A 102:15065-8. 2005
    ..Moreover, the approach permits dissection of the folding/unfolding free-energy changes into individual contributions from the peptide backbone and residue side chains...
  81. ncbi Methylene blue inhibits amyloid Abeta oligomerization by promoting fibrillization
    Mihaela Necula
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697, USA
    Biochemistry 46:8850-60. 2007
    ..If Abeta oligomers represent the primary pathogenic species, then inhibition of this highly toxic species via promotion of formation of less toxic aggregates may be therapeutically useful...
  82. ncbi Characterization of DLC1-SAM equilibrium unfolding at the amino acid residue level
    Shuai Yang
    Department of Chemistry, 3 Science Drive 3, Faculty of Science, National University of Singapore, Singapore 117543
    Biochemistry 48:4040-9. 2009
    ..Analysis of the midpoints of the transitions shows that the unfolding of the native state and formation of the denatured state are not cooperative and the unfolding of only a few residues seems to follow a two-state mechanism...
  83. ncbi Stability and dynamics of the porcine odorant-binding protein
    Maria Staiano
    Institute of Protein Biochemistry, CNR, Naples, Italy
    Biochemistry 46:11120-7. 2007
    ....
  84. ncbi A new method for determining the constant-pressure heat capacity change associated with the protein denaturation induced by guanidinium chloride (or urea)
    Ritu Singh
    Department of Biosciences, Jamia Millia Islamia, New Delhi 110 025, India
    Biophys Chem 133:81-9. 2008
    ..DeltaC(p)(D) of the protein is also compared with that estimated using the known heat capacities of amino acid residues and their fractional area exposed on denaturation...
  85. ncbi Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins
    Matthew J Cuneo
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    J Mol Biol 389:157-66. 2009
    ..We provide a model in which the energetics of long-range conformational equilibria controls subsite occupancy and ligand binding...
  86. pmc The effect of engineered disulfide bonds on the stability of Drosophila melanogaster acetylcholinesterase
    Omid Ranaei Siadat
    IPBS CNRS 205 route de Narbonne, Toulouse, France
    BMC Biochem 7:12. 2006
    ..Drosophila acetylcholinesterase is the most sensitive enzyme known and has been improved by in vitro mutagenesis. However, its stability has to be improved for extensive utilization...
  87. pmc Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale
    Matthieu Gaudet
    Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom
    Protein Sci 19:1544-54. 2010
    ..The results show that 75% of the interaction energy between FKBP-12 and rapamycin originates from residue Phe99 in the binding site...
  88. ncbi Residue-specific description of non-native transient structures in the ensemble of acid-denatured structures of the all-beta protein c-src SH3
    Heike I Rösner
    Structure Biology and NMR Laboratory, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, DK 2200 Copenhagen N, Denmark
    Biochemistry 49:3246-53. 2010
    ....
  89. pmc Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase
    J P Junker
    Physik Department E22, Technische Universitat Munchen, D 85748 Garching, Germany
    Biophys J 89:L46-8. 2005
    ..These experiments demonstrate that protein mechanics can be used to probe the substrate binding status of an enzyme...
  90. pmc Ligand binding modulates the mechanical stability of dihydrofolate reductase
    Sri Rama Koti Ainavarapu
    Department of Biological Sciences, Columbia University, New York, NY, USA
    Biophys J 89:3337-44. 2005
    ..Our observations support the view that the rate-limiting step in protein degradation by adenosine triphosphate-dependent proteases is the mechanical unfolding of the target protein...
  91. pmc From the Cover: Charge interactions can dominate the dimensions of intrinsically disordered proteins
    Sonja Müller-Späth
    Biochemisches Institut, Universitat Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Proc Natl Acad Sci U S A 107:14609-14. 2010
    ..The pronounced effect of charges on the dimensions of unfolded proteins has important implications for the cellular functions of IDPs...
  92. pmc Prion disease susceptibility is affected by beta-structure folding propensity and local side-chain interactions in PrP
    M Qasim Khan
    Campbell Family Institute for Cancer Research, Department of Biochemistry, University of Toronto, Toronto Medical Discovery Tower 4 307, 101 College Street, Toronto, ON, Canada M5G 1L7
    Proc Natl Acad Sci U S A 107:19808-13. 2010
    ..Removal of this capping motif increases the β-structure folding propensity of rabbit PrP to match that of PrP from mouse, a species more susceptible to prion disease...
  93. ncbi The equilibrium unfolding intermediate observed at pH 4 and its relationship with the kinetic folding intermediates in green fluorescent protein
    Sawako Enoki
    Department of Physics, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    J Mol Biol 361:969-82. 2006
    ..0. These results indicate that the equilibrium unfolding intermediate is composed of an ensemble of the folding intermediate species accumulated during the folding reaction, and thus support a hierarchical model of protein folding...
  94. ncbi The prominent conformational plasticity of lactoperoxidase: a chemical and pH stability analysis
    Barbara Boscolo
    Dipartimento di Chimica I F M, Universita di Torino, Torino, Italy
    Biochim Biophys Acta 1794:1041-8. 2009
    ..Overall, LPO is characterised by a high degree of peripheral structural plasticity without perturbation of the core heme moiety. The possible physiological meaning of such features is discussed...
  95. ncbi Detection of initiation sites in protein folding of the four helix bundle ACBP by chemical shift analysis
    Kristofer Modig
    Structural Biology and NMR Laboratory, Department of Molecular Biology, University of Copenhagen, Copenhagen Biocenter, Ole Maaløes Vej 5, DK 2200, Copenhagen, Denmark
    FEBS Lett 581:4965-71. 2007
    ..These findings strongly suggest transient formation of short helix-like segments, and identify unique sequence segments important for protein folding...
  96. ncbi Formation of on- and off-pathway intermediates in the folding kinetics of Azotobacter vinelandii apoflavodoxin
    Yves J M Bollen
    Department of Agrotechnology and Food Sciences, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, NL 6703 HA Wageningen, The Netherlands
    Biochemistry 43:10475-89. 2004
    ..The presence of such on-pathway and off-pathway intermediates in the folding kinetics of alpha-beta parallel proteins is apparently governed by protein topology...
  97. pmc Force-induced unfolding of fibronectin in the extracellular matrix of living cells
    Michael L Smith
    Laboratory for Biologically Oriented Materials, Department of Materials, Swiss Federal Institute of Technology Zurich, Zurich, Switzerland
    PLoS Biol 5:e268. 2007
    ..This could imply that Fn might play a significant role in mechanotransduction processes...
  98. pmc Differences in the pathways of proteins unfolding induced by urea and guanidine hydrochloride: molten globule state and aggregates
    Olga I Povarova
    Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology of the Russian Academy of Science, St Petersburg, Russia
    PLoS ONE 5:e15035. 2010
    ..That is why the increase in ANS fluorescence intensity is often recorded in the pathway of protein denaturation by GdnHCl, but not by urea...
  99. ncbi The rate of polyQ-mediated aggregation is dramatically affected by the number and location of surrounding domains
    Amy L Robertson
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
    J Mol Biol 413:879-87. 2011
    ..Our experimental data, together with a bioinformatic analysis of all human proteins possessing polyQ tracts, suggest that repeat location and protein domain architecture affect the disease susceptibility of human polyQ proteins...
  100. ncbi Adaptive evolution of p53 thermodynamic stability
    Kian Hoe Khoo
    MRC Centre for Protein Engineering, Hills Road, Cambridge CB2 0QH, UK
    J Mol Biol 393:161-75. 2009
    ..p53 from vertebrates has evolved to have a low thermodynamic stability and similarly short spontaneous half-life at organismal body temperature, which is related to function...
  101. pmc The denatured state dictates the topology of two proteins with almost identical sequence but different native structure and function
    Angela Morrone
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche A Rossi Fanelli, Universita di Roma La Sapienza, 5 00185 Rome, Italy
    J Biol Chem 286:3863-72. 2011
    ..Our results suggest a mechanism whereby protein topology is committed very early along the folding pathway, being imprinted in the residual structure of the denatured state...

Research Grants62

  1. THERMODYNAMICS OF PROTEIN DENATURATION
    DAVID BOLEN; Fiscal Year: 2003
    ..Our ultimate aim is to provide a strong foundation for thermodynamic measurements of protein stability, a foundation that at present is weak or invalid for many proteins. ..
  2. HIFU-enhanced tumor vaccines
    Chandan Guha; Fiscal Year: 2012
    ..Based upon these results, we hypothesized that exposure of solid tumors to "low" energy HIFU would induce protein denaturation, followed by proteosomal degradation of denatured proteins and an eventual increase in the amount of ..
  3. Novel Hsp90 Inhibitors to Reduce Misfolded Proteins in Alzheimer's Disease
    Mary L Michaelis; Fiscal Year: 2011
    ..hub for regulation of multi-chaperone complexes that allows for cell specific responses to stresses and protein denaturation. Evidence suggests that inhibitors of Hsp90 activate specific chaperone complexes, namely Hsp90, Hsp70, and ..
  4. Heat Shock Protein Therapeutics for Stroke
    Frank R Sharp; Fiscal Year: 2013
    ..many stresses including ischemia and assist protein folding during protein synthesis and re-folding after protein denaturation. Hsp70 protein expression is neuroprotective in a variety of models using a number of different methods of ..
  5. Intracellular targeting Hsp70 for pulmonary cytoprotection after toxin inhalation
    Richard Weisbart; Fiscal Year: 2013
    ..3E10, is an intracellular transporter that can deliver Hsp70 directly into cells where it minimizes protein denaturation and aggregation caused by oxidative stress...
  6. A Melamine Field Sensor for Surety Control of Dairy Products
    Kevin M Spencer; Fiscal Year: 2010
    ..It will be shown that a simple one-step protein denaturation is the only preprocessing required before measurement...
  7. An Instrument to Determine Protein Stability for Biologics Formulations Testing
    RICHARD KENNETH BROWN; Fiscal Year: 2013
    ..available instrument, this instrument will be capable of automatically preparing and analyzing up to 96 protein denaturation curves - enough to complete an entire formulations study for a new biologic...
  8. Conformal laser thermal therapy for epithelial cancer
    Brett E Bouma; Fiscal Year: 2010
    ..will be investigated for dynamic monitoring of the microscopic consequences of thermal excitation and protein denaturation. In order to control treatment volume, we will exploit the wavelength- dependence of water absorption in ..
  9. SCRUB TYPHUS VARIATION: PROTEINS AND MEMBRANE ANTIGENS
    Barbara Hanson; Fiscal Year: 1990
    ..epitopes identified with monoclonal antibodies by testing their reactivity with antibody before and after protein denaturation or treatment to destroy protein or carbohydrate moieties...
  10. MARKERS OF CNS INJURY
    Frank Sharp; Fiscal Year: 2006
    ..Heat shock proteins should also decrease protein denaturation and increase free ubiquitin in cells as assessed by Western blots...
  11. INTEGRATED ULTRACRYOMICROTOME SYSTEM
    David Sabatini; Fiscal Year: 2001
    ..the use of the chemical fixatives, organic solvents or resin polymerization conditions that cause extensive protein denaturation and loss of antigenic epitopes...
  12. CARDIAC PROTEIN SYNTHESIS: ISCHEMIA, OVERLOAD & ETHANOL
    SIDNEY SCHREIBER; Fiscal Year: 1991
    ..This reperfusion may accelerate cell damage with protein denaturation, and maintenance of normal protein synthesis to replace damaged and denatured proteins is essential to ..
  13. Arterial-Mimetic Grants Molded from Purified Proteins
    DAVID MASTERS; Fiscal Year: 2003
    ..Pure collagen and elastin is mixed together to create a non-woven material without protein denaturation. This material has the capacity to act as a therapeutic delivery system to allow biochemical and ..
  14. STABILITY AND STRUCTURE OF GP 120 ON INACTIVATED HIV
    Haynes Sheppard; Fiscal Year: 1999
    ..is the loss of the "native" oligomeric structure of the gp 120 during inactivation steps that involve protein denaturation (e.g. formalin)...
  15. GERIATRIC DENTISTRY FOR THE ALZHEIMER'S PATIENT
    Anthony Iacopino; Fiscal Year: 1990
    ..of calcium, all of which could result in an uncontrolled elevation of intracellular calcium leading to protein denaturation and eventual cell death; 2...
  16. The role of phosphorylation of a COPII coat protein, Sec31 in molecular export
    Pietro De Camilli; Fiscal Year: 2009
    ..mechanisms could account for the accumulation of such proteins: 1) increased misfolding, 2) enhanced protein denaturation and 3) decreased function in degradation pathways...
  17. Conformal laser thermal therapy for epithelial cancer
    Brett Bouma; Fiscal Year: 2007
    ..will be investigated for dynamic monitoring of the microscopic consequences of thermal excitation and protein denaturation. In order to control treatment volume, we will exploit the wavelength- dependence of water absorption in ..
  18. CELLULAR THERMOSTABILITY AND THERMOTOLERANCE
    JAMES LEPOCK; Fiscal Year: 2001
    ....
  19. Antioxidant function of MsrA in dopaminergic neurons
    Jean Christophe Rochet; Fiscal Year: 2007
    ..In turn, this information may stimulate the development of new therapeutic approaches in the treatment of age-related diseases of the brain. [unreadable] [unreadable]..
  20. Biopolymer Surfactants for Sealing Electroporated Membranes
    RAPHAEL CARL LEE; Fiscal Year: 2010
    ..Finally, we will conduct studies in parallel to determine if surfactant rescued cells manifest normal phenotype during proliferation or exhibit effects of mutations caused by unrepaired DMA. ..
  21. Membrane binding and aggregation of alpha-synuclein
    Jean Christophe Rochet; Fiscal Year: 2009
    ..Evidence that membrane-bound a-synuclein oligomers are valid drug targets would facilitate the development of screening assays to identify novel therapeutics. ..
  22. Conformational Properties of Protein Denatured States
    BRUCE BOWLER; Fiscal Year: 2009
    ..Our multipronged approach probes key parameters of protein denatured states expectedto be principal modulators of early events in protein folding. ..
  23. STRUCTURAL AND FUNCTIONAL STUDIES OF LEDGF
    Toshimichi Shinohara; Fiscal Year: 2002
    ..Studying the basic role of LEDGF in survival and death of LECs can enhance our standing of aging including development, growth, and age- related cataract. ..
  24. MEASUREMENT OF CHANGES IN DENATURED STATE ENERGETICS
    BRUCE BOWLER; Fiscal Year: 2003
    ..In sum, the proposed experiments use a novel thermodynamic approach to probe key parameters of protein denatured states expected to be principal modulators of early events in protein folding. ..
  25. ELECTRIC INJURY--IMAGING OF THERMAL/NON-THERMAL ASPECTS
    Raphael Lee; Fiscal Year: 2003
    ..to direct electrical mechanisms of cellular membrane damage (electroporation and electroconformational protein denaturation)...
  26. Cornea Preservation by Vitrification
    GREGORY FAHY; Fiscal Year: 2004
    ..In phase III we will launch commercial products and services. ..
  27. PLASMA PROTEIN PHENOTYPING OF PROTHROMBOTIC MICE
    Alvin Schmaier; Fiscal Year: 2003
    ..These studies could serve as a basis for the development of a global assay(s) to predict the prothrombotic state in man. (End of Abstract.) ..
  28. INTERACTION OF ATHEROGENIC LIPOPROTEINS WITH MACROPHAGES
    IRA A TABAS; Fiscal Year: 2010
    ..abstract_text> ..
  29. Mass Spectrometry and Enzyme Processes
    JULIE ANN LEARY; Fiscal Year: 2010
    ..Similarly, methods are proposedfor obtaining biological data on phosphoisomerase systems;one of which is highly difficult to classify kinetically and mechanistically. . . ..
  30. HERPESVIRUS PROTEIN SYNTHESIS AND VIRION ASSEMBLY
    D Gibson; Fiscal Year: 2008
    ..abstract_text> ..
  31. Normobaric Hyperoxia in Acute Ischemic Stroke
    Aneesh B Singhal; Fiscal Year: 2010
    ..Breathing high-flow oxygen may prove to be a simple, practical, portable, and potentially cost-effective therapy that improves stroke outcomes, either independently or by extending the time window for IV tPA. ..
  32. Mannosidase inhibitors as therapeutics for glycoprotein misfolding diseases
    Kelley W Moremen; Fiscal Year: 2010
    ....
  33. FORCES STABILIZING NATIVE PROTEIN STRUCTURES
    PETER PRIVALOV; Fiscal Year: 2003
    ..This project proposes to realize these objectives with combined investigations on several extended groups of DNA-binding proteins and their complexes. ..
  34. Antiproliferative actions of GATA-1 during hematopoiesis
    Mitchell Weiss; Fiscal Year: 2006
    ..through which GATA-1 orchestrates cell cycle arrest during normal hematopoietic differentiation and to apply this knowledge to the study of cytopenias and leukemia(s) associated with altered GATA-1 function [unreadable] [unreadable]..
  35. FOLDING OF THE INTRACELLULAR LIPID-BINDING PROTEINS
    Ira Ropson; Fiscal Year: 2003
    ..Single-site mutant proteins will be generated and studied for their effects on the transition state in protein folding. ..
  36. CFTR and interacting proteins from shark rectal gland
    John Riordan; Fiscal Year: 2003
    ....
  37. STRUCTURE AND ACTION OF STEROIDOGENIC REGULATORY PROTEIN
    HIMANGSHU BOSE; Fiscal Year: 2002
    ..Fulfilling these aims will provide the structural information needed to develop a detailed understanding of how StAR and MLN64 regulate cholesterol movement from the outer to inner mitochondrial membrane. ..
  38. SELF RECOGNITION IN GLOBULAR PROTEINS
    George Rose; Fiscal Year: 2001
    ..The chain is represented by simplified geometry and folds under the influence of an extremely simple energy function. Current results are encouraging. ..
  39. Proteomic Trajectory Mapping of Retinopathy of Prematurity
    Hiroyuki Matsumoto; Fiscal Year: 2008
    ..The results will contribute significantly to the development of drugs for the treatment of ROP and other related retinal diseases. [unreadable] [unreadable] [unreadable]..
  40. Clinical Algorithms to Monitor HIV Treatment in Uganda
    Lisa Spacek; Fiscal Year: 2008
    ..Richard Moore and HIV laboratory science with Dr. Thomas Quinn. My long-term goal is to become an independent investigator of HIV clinical epidemiology in resource-limited settings. ..
  41. Macrophage Death in Plaque Vulnerability
    Ira Tabas; Fiscal Year: 2005
    ..New insights in this area may suggest new therapeutic strategies to combat cardiovascular disease. ..
  42. Biocomplexity VII: Unraveling the Function and Kinetics
    Santiago Schnell; Fiscal Year: 2005
    ..The proceeding and discussion of conference will be published in a peer-refereed journal. ..
  43. Thrombostatin-A Thrombin Receptor Inhibitor
    Alvin Schmaier; Fiscal Year: 2006
    ..Second, the preparation of an IND application for its human use for one indication will be achieved. Thrombostatin(tm) has the opportunity to be a first-in-its-class thrombin receptor activation antagonist. [unreadable] [unreadable]..
  44. The Clinical Interface of Molecular and Cellular Biology
    David Perlmutter; Fiscal Year: 2006
    ....
  45. Southeast Collaboratory for High-Field Biomolecular NMR
    James Prestegard; Fiscal Year: 2006
    ..Target areas for future expansion include carbohydrates and metallo-proteins. ..
  46. ADAPT 78 IN OXIDANT STRESS, AGING AND NEURODEGENERATION
    Kelvin Davies; Fiscal Year: 2004
    ..abstract_text> ..
  47. Herpesvirus Proteinase - Possible Target for Antivirals
    D Gibson; Fiscal Year: 2007
    ..It is also likely that useful new information will be generated in the areas of viral proteinase mechanisms and herpesvirus replication. ..
  48. ANNEXIN PHOSPHORYLATION: CAUSES AND CONSEQUENCES
    CARL CREUTZ; Fiscal Year: 2004
    ....
  49. Liver Injury in Alpha-1-Antitrypsin Deficiency
    David Perlmutter; Fiscal Year: 2007
    ..Thus, the studies proposed in this competitive renewal application will address the novel concept that oxidative stress causes, or contributes to, liver cell injury in alpha-1-ATZ deficiency. ..
  50. Thermo Finnigan LTQ-Orbitrap
    Julie Leary; Fiscal Year: 2007
    ..A mechanism is in place to train and oversee student researchers and Professor Leary's group is available for consultation, advice and data interpretation. [unreadable] [unreadable] [unreadable]..
  51. Oral Yeast Carriage and Salivary Antifungal Activity
    EVA HELMERHORST; Fiscal Year: 2008
    ..The experiments outlined here will provide the basis for a better understanding of antifungal mechanisms exerted by saliva in the oral cavity. [unreadable] [unreadable] [unreadable]..
  52. Mannosidases in Glycoprotein Biosynthesis & Catabolism
    Kelley Moremen; Fiscal Year: 2008
    ..The fourth aim will test hypotheses for the individual roles and mechanisms of regulation of members of the ERAD targeting machinery by a novel real-time RT-PCR strategy and by siRNA approaches. ..
  53. Identifying Pathogenic Protein Aggregates in ALS through Autocatalytic Misfolding
    Patrick Bosque; Fiscal Year: 2008
    ..Further, if successful, the approach can be readily modified to use as an tool for high-throughput screening of potentially therapeutic compounds. [unreadable] [unreadable] [unreadable]..
  54. Multidisciplinary Training Program in Human Imaging
    GEOFFREY CLARKE; Fiscal Year: 2008
    ..abstract_text> ..
  55. LOCAL EXPRESSION OF ALPHA-1-ANTITRYPSIN IN EMPHYSEMA
    David Perlmutter; Fiscal Year: 2008
    ....
  56. Protein Structure Refinement Using a Novel Moveset
    Tobin Sosnick; Fiscal Year: 2009
    ..The proposed research involves a new, synergistic collaboration and novel computational techniques as indicated in the RFA. ..