ion transport

Summary

Summary: The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.

Top Publications

  1. ncbi KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron
    Hélène Louis-Dit-Picard
    Institut National de la Sante et de la Recherche Medicale INSERM, Unité Mixte de Recherche Scientifique UMRS 970, Paris Centre de Recherche Cardiovasculaire PARCC, Paris, France
    Nat Genet 44:456-60, S1-3. 2012
  2. ncbi Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH
    Carola Hunte
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Nature 435:1197-202. 2005
  3. pmc Structure of a eukaryotic CLC transporter defines an intermediate state in the transport cycle
    Liang Feng
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Science 330:635-41. 2010
  4. pmc A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport
    Florian Steinberg
    The Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 15:461-71. 2013
  5. ncbi Direct binding of the Na--H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation
    S P Denker
    Department of Stomatology, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Cell 6:1425-36. 2000
  6. pmc Regulation of TMEM16A chloride channel properties by alternative splicing
    Loretta Ferrera
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, Largo G Gaslini 5, 16147 Genova
    J Biol Chem 284:33360-8. 2009
  7. pmc Transport mechanism and pH regulation of the Na+/H+ antiporter NhaA from Escherichia coli: an electrophysiological study
    Thomas Mager
    Max Planck Institut fur Biophysik, 60438 Frankfurt Main, Germany
    J Biol Chem 286:23570-81. 2011
  8. pmc Alkali metal cation transport and homeostasis in yeasts
    Joaquin Arino
    Departament de Bioquimica i Biologia Molecular, Ed V, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Microbiol Mol Biol Rev 74:95-120. 2010
  9. ncbi Energetics of ion conduction through the K+ channel
    S Berneche
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Nature 414:73-7. 2001
  10. ncbi Facilitated ion transport in all-solid-state flexible supercapacitors
    Bong Gill Choi
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Daejeon 305 701, Republic of Korea
    ACS Nano 5:7205-13. 2011

Detail Information

Publications330 found, 100 shown here

  1. ncbi KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron
    Hélène Louis-Dit-Picard
    Institut National de la Sante et de la Recherche Medicale INSERM, Unité Mixte de Recherche Scientifique UMRS 970, Paris Centre de Recherche Cardiovasculaire PARCC, Paris, France
    Nat Genet 44:456-60, S1-3. 2012
    ..Our study establishes a role for KLHL3 as a new member of the complex signaling pathway regulating ion homeostasis in the distal nephron and indirectly blood pressure...
  2. ncbi Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH
    Carola Hunte
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Nature 435:1197-202. 2005
    ..This ion-exchange machinery is regulated by a conformational change elicited by a pH signal perceived at the entry to the cytoplasmic funnel...
  3. pmc Structure of a eukaryotic CLC transporter defines an intermediate state in the transport cycle
    Liang Feng
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Science 330:635-41. 2010
    ..Comparison with prokaryotic CLC shows that a gating glutamate residue changes conformation and suggests a basis for 2:1 Cl(-)/H(+) exchange and a simple mechanistic connection between CLC channels and transporters...
  4. pmc A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport
    Florian Steinberg
    The Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Nat Cell Biol 15:461-71. 2013
    ..Our data identify the SNX27-retromer as a major endosomal recycling hub required to maintain cellular nutrient homeostasis...
  5. ncbi Direct binding of the Na--H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation
    S P Denker
    Department of Stomatology, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Cell 6:1425-36. 2000
    ..We propose a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger...
  6. pmc Regulation of TMEM16A chloride channel properties by alternative splicing
    Loretta Ferrera
    Laboratory of Molecular Genetics, Istituto Giannina Gaslini, Largo G Gaslini 5, 16147 Genova
    J Biol Chem 284:33360-8. 2009
    ..Alternative splicing appears as an important mechanism to regulate the voltage and Ca(2+) dependence of the TMEM16A-dependent Cl(-) channels in a tissue-specific manner...
  7. pmc Transport mechanism and pH regulation of the Na+/H+ antiporter NhaA from Escherichia coli: an electrophysiological study
    Thomas Mager
    Max Planck Institut fur Biophysik, 60438 Frankfurt Main, Germany
    J Biol Chem 286:23570-81. 2011
    ..In conclusion, the electrophysiological assay allows the revelation of the mechanism of NhaA antiport and sheds new light on the concept of NhaA pH regulation...
  8. pmc Alkali metal cation transport and homeostasis in yeasts
    Joaquin Arino
    Departament de Bioquimica i Biologia Molecular, Ed V, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Microbiol Mol Biol Rev 74:95-120. 2010
    ....
  9. ncbi Energetics of ion conduction through the K+ channel
    S Berneche
    Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA
    Nature 414:73-7. 2001
    ..Ion-ion repulsion, although essential for rapid conduction, is shown to act only at very short distances. The calculations show also that the rapidly conducting pore is selective...
  10. ncbi Facilitated ion transport in all-solid-state flexible supercapacitors
    Bong Gill Choi
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Daejeon 305 701, Republic of Korea
    ACS Nano 5:7205-13. 2011
    ..Therefore, this research provides new insight into the rational design and fabrication of all-solid-state flexible energy-storage devices as well as the fundamental understanding of ion and charge transport at the interface...
  11. pmc Molecular dynamics of ion transport through the open conformation of a bacterial voltage-gated sodium channel
    Martin B Ulmschneider
    Institute of Structural and Molecular Biology, Birkbeck College, University of London, London WC1E 7HX, United Kingdom
    Proc Natl Acad Sci U S A 110:6364-9. 2013
    ..These studies simulate sodium channel conductance based on an experimentally determined structure of a sodium channel pore that has a completely open transmembrane pathway and activation gate...
  12. pmc Human induced pluripotent stem-derived retinal pigment epithelium (RPE) cells exhibit ion transport, membrane potential, polarized vascular endothelial growth factor secretion, and gene expression pattern similar to native RPE
    Maria Kokkinaki
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    Stem Cells 29:825-35. 2011
    ..Here, we show for the first time that RPE generated from hiPS cells under defined conditions exhibit ion transport, membrane potential, polarized vascular endothelial growth factor secretion, and gene expression profile ..
  13. ncbi Molecular physiology of SPAK and OSR1: two Ste20-related protein kinases regulating ion transport
    Kenneth B Gagnon
    Department of Anesthesiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2520, USA
    Physiol Rev 92:1577-617. 2012
    ....
  14. ncbi Nanoionics: ion transport and electrochemical storage in confined systems
    J Maier
    Max Planck Institut fur Festkorperforschung, 70569 Stuttgart, Germany
    Nat Mater 4:805-15. 2005
    ..this potential implies both emphasizing the indispensability of electrochemical devices that rely on ion transport and complement the world of electronics, and working out the drastic impact of interfaces and size effects on ..
  15. ncbi Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
    H Luecke
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Science 286:255-61. 1999
    ..The structural changes detected suggest the means for conserving energy at the active site and for ensuring the directionality of proton translocation...
  16. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
    ..This review highlights the approaches, major findings and unresolved problems in alkaline pH homeostasis, focusing on the small number of well-characterized alkali-tolerant and extremely alkaliphilic bacteria...
  17. ncbi The great big alveolar TI cell: evolving concepts and paradigms
    Leland G Dobbs
    Cardiovascular Research Institute, University of California, San Francisco, USA
    Cell Physiol Biochem 25:55-62. 2010
    ..TI cells contain the molecular machinery necessary for ion transport and take up Na(+), K(+), and Cl(-), from which one can infer that it is likely that they play a role in ion ..
  18. pmc A high-density association screen of 155 ion transport genes for involvement with common migraine
    Dale R Nyholt
    Genetic Epidemiology Laboratory, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane4029, Queensland, Australia
    Hum Mol Genet 17:3318-31. 2008
    ..migraine subtypes, and the fact that all three FHM genes are involved in the transport of ions, suggest that ion transport genes may underlie susceptibility to common forms of migraine...
  19. ncbi Energetic optimization of ion conduction rate by the K+ selectivity filter
    J H Morais-Cabral
    Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, New Haven, Connecticut 06520, USA
    Nature 414:37-42. 2001
    ..The energetic balance between these configurations is a clear example of evolutionary optimization of protein function...
  20. ncbi Intracellular gate opening in Shaker K+ channels defined by high-affinity metal bridges
    Sarah M Webster
    Department of Neurobiology, Harvard Medical School, 220 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 428:864-8. 2004
    ..A narrower opening of the bundle crossing in Shaker K+ channels may help to explain why Shaker has an approximately tenfold lower conductance than its bacterial relatives...
  21. pmc Transepithelial ion transport is suppressed in hypoxic sinonasal epithelium
    Angela Blount
    Department of Surgery, Division of Otolaryngology Head and Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Laryngoscope 121:1929-34. 2011
    ....
  22. ncbi A novel missense mutation in the sodium bicarbonate cotransporter (NBCe1/SLC4A4) causes proximal tubular acidosis and glaucoma through ion transport defects
    Dganit Dinour
    Department of Nephrology and Hypertension, Chaim Sheba Medical Center, Tel Hashomer, and Tel Aviv University, 52621 Israel
    J Biol Chem 279:52238-46. 2004
    ....
  23. ncbi Ion conduction pore is conserved among potassium channels
    Z Lu
    Department of Physiology, University of Pennsylvania, Philadelphia 19104, USA
    Nature 413:809-13. 2001
    ..The resulting chimaeras retain the respective functional hallmarks of the eukaryotic channels, which indicates that the ion conduction pore is indeed conserved among K+ channels...
  24. pmc Loss of TMEM16A causes a defect in epithelial Ca2+-dependent chloride transport
    Jiraporn Ousingsawat
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, D 93053 Regensburg, Germany
    J Biol Chem 284:28698-703. 2009
    ..These results clearly demonstrate the broad physiological role of TMEM16A(-/-) for Ca(2+)-dependent Cl(-) secretion and provide the basis for novel treatments in cystic fibrosis, infectious diarrhea, and Sjöegren syndrome...
  25. pmc Crystal structure of a potassium ion transporter, TrkH
    Yu Cao
    Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, New York 10032, USA
    Nature 471:336-40. 2011
    ..These results reveal the molecular basis of K(+) selectivity and suggest a novel gating mechanism for this large and important family of membrane transport proteins...
  26. ncbi Regulation of airway surface liquid volume and mucus transport by active ion transport
    Robert Tarran
    Thurston Bowles Building, The University of North Carolina, Chapel Hill, NC 27599 7248, USA
    Proc Am Thorac Soc 1:42-6. 2004
    ..Whether airway surface liquid (ASL) volume is maintained by passive surface forces or by active ion transport is controversial yet crucial to the understanding of how this system operates in both health and disease...
  27. pmc Exposure to cigarette smoke condensate reduces calcium activated chloride channel transport in primary sinonasal epithelial cultures
    Frank W Virgin
    Department of Surgery Division of Otolaryngology, University of Alabama at Birmingham, Alabama 35294, USA
    Laryngoscope 120:1465-9. 2010
    ..Because it is not known whether CaCCs are also inhibited by tobacco exposure, the current study was designed to investigate the effect of cigarette smoke condensate (CSC) on CaCC transport...
  28. ncbi Role of ion transport in control of apoptotic cell death
    Florian Lang
    Institute of Physiology, University of Tubingen, Tubingen, Germany
    Compr Physiol 2:2037-61. 2012
    Cell shrinkage is a hallmark and contributes to signaling of apoptosis. Apoptotic cell shrinkage requires ion transport across the cell membrane involving K(+) channels, Cl(-) or anion channels, Na(+)/H(+) exchange, Na(+),K(+),Cl(-) ..
  29. pmc Characteristics and possible functions of mitochondrial Ca(2+) transport mechanisms
    Thomas E Gunter
    Department of Biochemistry and Biophysics and Mitochondrial Research and Innovation Group, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochim Biophys Acta 1787:1291-308. 2009
    ..The characteristics of these mechanisms of Ca(2+) transport and a discussion of how they might function are described in this paper...
  30. ncbi Mitochondrial ion transport pathways: role in metabolic diseases
    Ariel R Cardoso
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil
    Biochim Biophys Acta 1797:832-8. 2010
    ..However, in many experimental settings, the relationship between the activity of mitochondrial ion transport and metabolic disorders is still poorly understood...
  31. ncbi Bicarbonate transport in cell physiology and disease
    Emmanuelle Cordat
    Membrane Protein Research Group, Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
    Biochem J 417:423-39. 2009
    ..Defects of bicarbonate transport proteins manifest in diseases of most organ systems. Fourteen gene products facilitate mammalian bicarbonate transport, whose physiology and pathophysiology is discussed in the present review...
  32. pmc Tmem16A encodes the Ca2+-activated Cl- channel in mouse submandibular salivary gland acinar cells
    Victor G Romanenko
    Department of Pharmacology and Physiology, University of Rochester, Rochester, New York 14642, USA
    J Biol Chem 285:12990-3001. 2010
    ....
  33. ncbi Human copper homeostasis: a network of interconnected pathways
    Svetlana Lutsenko
    Department of Physiology, Johns Hopkins University, Baltimore, MD 21205, USA
    Curr Opin Chem Biol 14:211-7. 2010
    ..It has become apparent that the copper trafficking machinery is regulated at several levels and that the cross-talk between cell compartments contributes to the intracellular copper balance. The human copper regulon is emerging...
  34. ncbi Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae
    Jiun Lin Horng
    Institute of Cellular and Organismic Biology, Academia Sinica, Taipei
    Am J Physiol Cell Physiol 297:C845-54. 2009
    ..This study provides direct and convincing evidence for Cl- transport by MRCs of SW- and FW-acclimated euryhaline tilapia and the involvement of an apical NCC in Cl- uptake of MRCs of FW-acclimated fish...
  35. ncbi Comparison of vectorial ion transport in primary murine airway and human sinonasal air-liquid interface cultures, models for studies of cystic fibrosis, and other airway diseases
    Shaoyan Zhang
    Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Am J Rhinol Allergy 23:149-52. 2009
    The purpose of this study was to compare vectorial ion transport within murine trachea, murine nasal septa, and human sinonasal cultured epithelium...
  36. pmc Na+-dependent HCO3- import by the slc4a10 gene product involves Cl- export
    Helle Hasager Damkier
    Water and Salt Research Center, Department of Anatomy, Aarhus University, DK 8000 Aarhus, Denmark
    J Biol Chem 285:26998-7007. 2010
    ..In conclusion, the data suggest that slc4a10 expressed in mammalian cells encodes a Na(+)-dependent Cl(-)/HCO(3)(-) exchanger in which four specific charged amino acids seem necessary for ion transport.
  37. ncbi Epithelial mitochondria-rich cells and associated innervation in adult and developing zebrafish
    Michael G Jonz
    Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1
    J Comp Neurol 497:817-32. 2006
    ..We characterized the potential innervation of MRCs in various gill and extrabranchial tissues involved in ion transport in the model vertebrate the zebrafish...
  38. pmc Energetics of ion conduction through the gramicidin channel
    Toby W Allen
    Departments of Physiology and Biophysics and Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:117-22. 2004
    ..Small changes in the potential function, including contributions from electronic polarization, are likely to be sufficient to obtain quantitative agreement with experiments...
  39. pmc Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae
    A M Marini
    Laboratoire de Physiologie Cellulaire et de Genetique des Levures, Universite Libre de Bruxelles, Belgium
    EMBO J 13:3456-63. 1994
    ..Its main function appears to be to enable cells grown under nitrogen-limiting conditions to incorporate ammonium present at relatively low concentrations in the growth medium...
  40. pmc Functional ion channels in pulmonary alveolar type I cells support a role for type I cells in lung ion transport
    Meshell D Johnson
    Department of Medicine, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 103:4964-9. 2006
    ..2 mum) liquid layer lining the alveolar epithelium. Fluid fluxes are regulated by ion transport across the alveolar epithelium, which is composed of alveolar type I (TI) and type II (TII) cells...
  41. pmc A microscopic view of ion conduction through the K+ channel
    Simon Berneche
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 100:8644-8. 2003
    ..Analysis of the ion-conduction process reveals a lack of equivalence between the cation-binding sites in the selectivity filter...
  42. ncbi Measurement of ion transport function in rectal biopsies
    Martin J Hug
    Pharmacy, University Medical Center Freiburg, D 79106, Freiburg, Germany
    Methods Mol Biol 741:87-107. 2011
    ..functions as an anion channel and is known to interact with a number of other cellular proteins involved in ion transport. To date more than 1,800 mutations are known, most of which result in various degrees of impaired transport ..
  43. pmc Structural perspectives on secondary active transporters
    Olga Boudker
    Weill Cornell Medical College, 1300 York Ave, New York, NY 10021, USA
    Trends Pharmacol Sci 31:418-26. 2010
    ..We also discuss the structural basis of substrate binding, ion coupling and inhibition viewed from the perspective of these similarities...
  44. pmc Mechanism of Cu+-transporting ATPases: soluble Cu+ chaperones directly transfer Cu+ to transmembrane transport sites
    Manuel González-Guerrero
    Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Road, Worcester, MA 01609, USA
    Proc Natl Acad Sci U S A 105:5992-7. 2008
    ..These data are consistent with a model where MBDs serve a regulatory function without participating in metal transport and the chaperone delivers Cu(+) directly to transmembrane transport sites of Cu(+)-ATPases...
  45. ncbi New insights into fish ion regulation and mitochondrion-rich cells
    Pung Pung Hwang
    Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, 115, Taiwan
    Comp Biochem Physiol A Mol Integr Physiol 148:479-97. 2007
    ..on fish osmoregulation and ionoregulation, provide an excellent model to study the regulatory mechanisms of ion transport. The present review introduces and discusses some recent advances in relevant issues of teleost gill ion ..
  46. ncbi Cortisol regulates Na+ uptake in zebrafish, Danio rerio, larvae via the glucocorticoid receptor
    Yusuke Kumai
    Department of Biology, University of Ottawa, Ottawa, ON, Canada
    Mol Cell Endocrinol 364:113-25. 2012
    ....
  47. pmc Molecular mechanisms underlying the corneal endothelial pump
    Joseph A Bonanno
    Indiana University, School of Optometry, 800 E Atwater Avenue, Bloomington, IN 47405, USA
    Exp Eye Res 95:2-7. 2012
    ..Although the conditions under which the "Pump" is supported are known, a complete model of the endothelial "Pump" has yet to emerge...
  48. pmc Actions of hydrogen sulphide on ion transport across rat distal colon
    B Hennig
    Institute for Veterinary Physiology, Justus Liebig University Giessen, Giessen, Germany
    Br J Pharmacol 158:1263-75. 2009
    The aim of this study was to identify the actions of H(2)S on ion transport across rat distal colon.
  49. pmc Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter
    Katia Herz
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    J Biol Chem 285:2211-20. 2010
    ..5 (10-fold) but not at pH 8.5, implying the importance of Asp(65) negative charge for pH activation of the antiporter...
  50. ncbi Effects of carbon monoxide on ion transport across rat distal colon
    Julia Steidle
    Institut für Veterinär Physiologie, Universität Gieβen, Frankfurter Str 100, 35392 Giessen, Germany
    Am J Physiol Gastrointest Liver Physiol 300:G207-16. 2011
    The aim of the present study was to investigate whether carbon monoxide (CO) induces changes in ion transport across the distal colon of rats and to study the mechanisms involved...
  51. ncbi TMEM16A confers receptor-activated calcium-dependent chloride conductance
    Young Duk Yang
    Sensory Research Center, CRI, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    Nature 455:1210-5. 2008
    ..We conclude that ANO1 is a candidate Ca(2+)-activated chloride channel that mediates receptor-activated chloride currents in diverse physiological processes...
  52. ncbi Molecular mechanisms of ischemic cerebral edema: role of electroneutral ion transport
    Kristopher T Kahle
    Departments of Neurosurgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Physiology (Bethesda) 24:257-65. 2009
    ..Combination pharmacological strategies that include NKCC1 as a target might thus prove beneficial for the treatment of ischemic, and potentially other types of, cerebral edema...
  53. ncbi A thallium-sensitive, fluorescence-based assay for detecting and characterizing potassium channel modulators in mammalian cells
    C David Weaver
    Vanderbilt Institute of Chemical Biology and Department of Pharmacology, 812 Robinson Research Building, Nashville, TN 37232 6600, USA
    J Biomol Screen 9:671-7. 2004
    ....
  54. ncbi Purinergic P2Y6 receptors induce Ca2+ and CFTR dependent Cl- secretion in mouse trachea
    Rainer Schreiber
    Institut fur Physiologie, Universitat Regensburg, Germany
    Cell Physiol Biochem 16:99-108. 2005
    ..In summary, activation of luminal P2Y6 receptors in the airways shifts electrolyte transport towards secretion by increasing intracellular Ca+ and activation of PKA...
  55. pmc Ion transport by pulmonary epithelia
    Monika I Hollenhorst
    Institute of Animal Physiology, Justus Liebig University Giessen, Wartweg 95, 35392 Giessen, Germany
    J Biomed Biotechnol 2011:174306. 2011
    ..Therefore, the epithelial cells are equipped with a wide variety of ion transport proteins, among which Na⁺, Cl⁻, and K⁺ channels have been identified to play a role in the regulation of ..
  56. ncbi Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli
    D Zuber
    Max Planck Institut fur Biophysik, Max von Laue Strasse 3, D 60438 Frankfurt Main, Germany
    Biochim Biophys Acta 1709:240-50. 2005
    ..3 mM for Li+ at basic pH and 180 mM for Na+ and 50 mM for Li+ at neutral pH. The data demonstrate that in the passive downhill uptake mode pH regulation of the carrier affects both apparent Km as well as turnover (Vmax)...
  57. pmc Cyclic nucleotide-gated channels on the flagellum control Ca2+ entry into sperm
    B Wiesner
    Forschungsinstitut fur Molekulare Pharmakologie, D 10315 Berlin
    J Cell Biol 142:473-84. 1998
    ....
  58. ncbi Structure and mechanism of the lactose permease of Escherichia coli
    Jeff Abramson
    Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK
    Science 301:610-5. 2003
    ..We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data...
  59. ncbi Ex vivo CF diagnosis by intestinal current measurements (ICM) in small aperture, circulating Ussing chambers
    Hugo R de Jonge
    Departments of Paediatric Gastroenterology and Biochemistry, Erasmus University Medical Centre, P O Box 1738, Rotterdam, 3000DR The Netherlands
    J Cyst Fibros 3:159-63. 2004
    ..The ICM technique allows the registration of CF-induced changes in electrogenic transepithelial ion transport (Cl-, HCO3-, K+) in a Cl- secretory epithelium, and on the basis of pharmacological criteria, is able to ..
  60. ncbi The Na+ transporter AtHKT1;1 controls retrieval of Na+ from the xylem in Arabidopsis
    Romola Jane Davenport
    Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, UK
    Plant Cell Environ 30:497-507. 2007
    ..The hkt1;1 mutation has been shown previously to rescue growth of the sos1 mutant on low K(+); however, HKT1;1 knockout did not alter K(+) or (86)Rb(+) accumulation in sos1...
  61. pmc Hesperidin stimulates cystic fibrosis transmembrane conductance regulator-mediated chloride secretion and ciliary beat frequency in sinonasal epithelium
    Christopher Azbell
    Department of Surgery Division of Otolaryngology, University of Alabama at Birmingham, Birmingham, AL, USA
    Otolaryngol Head Neck Surg 143:397-404. 2010
    ....
  62. pmc STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane
    Shenyuan L Zhang
    Department of Physiology and Biophysics and Center for Immunology, University of California, Irvine, California 92697, USA
    Nature 437:902-5. 2005
    ..We propose that STIM1 functions as the missing link between Ca2+ store depletion and SOC influx, serving as a Ca2+ sensor that translocates upon store depletion to the plasma membrane to activate CRAC channels...
  63. ncbi SSM-based electrophysiology
    Patrick Schulz
    Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt Main, Germany
    Methods 46:97-103. 2008
    ..Its rugged design and potential for automation make it suitable for drug screening...
  64. pmc SLC41A1 is a novel mammalian Mg2+ carrier
    Martin Kolisek
    Institute of Veterinary Physiology, FU Berlin, Oertzenweg 19b, D 14163 Berlin, Germany
    J Biol Chem 283:16235-47. 2008
    ..SLC41A1 activity is temperature-sensitive but not sensitive to the only known magnesium channel blocker, cobalt(III) hexaammine. Taken together, these data functionally identify SLC41A1 as a mammalian carrier mediating magnesium efflux...
  65. ncbi Identification of the Escherichia coli K-12 Nramp orthologue (MntH) as a selective divalent metal ion transporter
    H Makui
    INRS Institut Armand Frappier, 531 Bd des Prairies, Laval H7V 1B7, PQ, Canada
    Mol Microbiol 35:1065-78. 2000
    ..coli. This study indicates that proton-dependent divalent metal ion uptake has been preserved in the Nramp family from bacteria to humans...
  66. pmc DeltaF508 CFTR processing correction and activity in polarized airway and non-airway cell monolayers
    S M Rowe
    Department of Medicine, University of Alabama at Birmingham, 1530 3rd Ave South, Birmingham, AL 35294 0005, United States
    Pulm Pharmacol Ther 23:268-78. 2010
    ..The results identify important differences in DeltaF508 CFTR activation in polarizing epithelial models of CF, and have important implications regarding detection of rescued of DeltaF508 CFTR in vivo...
  67. pmc The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane
    J Wieland
    Institut für Biochemie der Universität Stuttgart, Germany
    EMBO J 14:3870-82. 1995
    ..Finally, we show that the Na+ tolerance mediated through these pumps is regulated by calmodulin via a calcineurin-independent mechanism which activates the Pmr2 ion pumps post-transcriptionally...
  68. ncbi The p-type ATPase superfamily
    Henry Chan
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA, USA
    J Mol Microbiol Biotechnol 19:5-104. 2010
    ..The classification of P-type ATPases of unknown function into phylogenetic families provides guides for future molecular biological studies...
  69. pmc CLC channels and transporters: proteins with borderline personalities
    Alessio Accardi
    Department of Molecular Physiology and Biophysics, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Biochim Biophys Acta 1798:1457-64. 2010
    ..This review focuses on the recent mechanistic insights on the how the structural similarities between CLC channels and transporters translate in unexpected mechanistic analogies between these two sub-classes...
  70. ncbi Early death due to defective neonatal lung liquid clearance in alpha-ENaC-deficient mice
    E Hummler
    Institut de Pharmacologie et de Toxicologie de l Université, Lausanne, Switzerland
    Nat Genet 12:325-8. 1996
    ..Alpha-ENaC(-/-) neonates developed respiratory distress and died within 40 h of birth from failure to clear their lungs of liquid. This study shows that ENaC plays a critical role in the adaptation of the newborn lung to air breathing...
  71. ncbi Mechanism of Na+/H+ antiporting
    Isaiah T Arkin
    D E Shaw Research, New York, NY 10036, USA
    Science 317:799-803. 2007
    ..Additional mutagenesis experiments further validated the model...
  72. ncbi The structural basis of calcium transport by the calcium pump
    Claus Olesen
    Centre for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, University of Aarhus, Ole Worms Alle, blg 1185, DK 8000 Aarhus C, Denmark
    Nature 450:1036-42. 2007
    ..The mechanism explains how P-type ATPases are able to form the steep electrochemical gradients required for key functions in eukaryotic cells...
  73. ncbi Carbon monoxide rapidly impairs alveolar fluid clearance by inhibiting epithelial sodium channels
    Mike Althaus
    Department of Internal Medicine, University of Giessen Lung Center, Justus Liebig University, Aulweg 123 Room 6 11, D 35392 Giessen, Germany
    Am J Respir Cell Mol Biol 41:639-50. 2009
    ..No study has assessed the effects of CO on transepithelial ion transport and alveolar fluid reabsorption, two key aspects of alveolocapillary barrier function that are perturbed in ..
  74. ncbi Ion uptake and acid secretion in zebrafish (Danio rerio)
    Pung Pung Hwang
    Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China
    J Exp Biol 212:1745-52. 2009
    ....
  75. ncbi Novel gating and sensitizing mechanism of capsaicin receptor (TRPV1): tonic inhibitory regulation of extracellular sodium through the external protonation sites on TRPV1
    Toshio Ohta
    Laboratory of Pharmacology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Biol Chem 283:9377-87. 2008
    ....
  76. ncbi Endolymphatic sodium homeostasis by Reissner's membrane
    J H Lee
    Kansas State University, Department of Anatomy and Physiology, 126 Coles Hall, 1600 Denison Ave, Manhattan, KS 66506 5802, USA
    Neuroscience 119:3-8. 2003
    ..These results provide the first direct evidence that Reissner's membrane contributes to normal cochlear function by absorption of Na(+) from endolymph...
  77. ncbi Escherichia coli acid resistance: tales of an amateur acidophile
    John W Foster
    Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, Alabama 36695, USA
    Nat Rev Microbiol 2:898-907. 2004
    ..Surprisingly, certain microorganisms that grow at neutral pH have elegantly regulated systems that enable survival during excursions into acidic environments. The best-characterized acid-resistance system is found in E. coli...
  78. pmc 17beta-Estradiol inhibits Ca2+-dependent homeostasis of airway surface liquid volume in human cystic fibrosis airway epithelia
    Ray D Coakley
    Cystic Fibrosis Pulmonary Research and Treatment Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Clin Invest 118:4025-35. 2008
    ....
  79. pmc Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport
    Tim T Lambers
    Department of Physiology, Radboud University Nijmegen Medical Centre, The Netherlands
    EMBO J 25:2978-88. 2006
    ..In summary, CaBP(28K) acts in Ca(2+)-transporting epithelia as a dynamic Ca(2+) buffer, regulating [Ca(2+)] in close vicinity to the TRPV5 pore by direct association with the channel...
  80. pmc Alveolar edema fluid clearance and acute lung injury
    Yves Berthiaume
    Département de médecine et Centre de recherche du Centre Hospitalier de l Université de Montréal, Universite de Montreal, Montreal, Quebec, Canada
    Respir Physiol Neurobiol 159:350-9. 2007
    ..Vectorial ion transport across the alveolar and distal airway epithelia is the primary determinant of alveolar fluid clearance...
  81. ncbi Potassium transporters in plants--involvement in K+ acquisition, redistribution and homeostasis
    Markus Gierth
    Universitat zu Koln, Institut für Botanik II, Gyrhofstrasse 15, 50931 Koln, Germany
    FEBS Lett 581:2348-56. 2007
    ..This review will discuss the contribution of members of each family to potassium acquisition, redistribution and homeostasis...
  82. ncbi Expression of bestrophin-1, the product of the VMD2 gene, modulates voltage-dependent Ca2+ channels in retinal pigment epithelial cells
    Rita Rosenthal
    Augenklinik, Charite Universitaetsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany
    FASEB J 20:178-80. 2006
    ....
  83. ncbi Purinergic signalling in epithelial ion transport: regulation of secretion and absorption
    I Novak
    Department of Biology, August Krogh Building, University of Copenhagen, Denmark
    Acta Physiol (Oxf) 202:501-22. 2011
    ..nucleotides/nucleosides are also extracellular molecules that regulate diverse cellular functions, including ion transport. In this review, I will first introduce the main components of the extracellular ATP signalling, which have ..
  84. ncbi Sodium influence on energy transduction by complexes I from Escherichia coli and Paracoccus denitrificans
    Ana P Batista
    Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Av de Republica EAN, 2780 157 Oeiras, Portugal
    Biochim Biophys Acta 1807:286-92. 2011
    ..marinus enzyme. Nevertheless, the Na(+)/H(+) antiport activity seems not to be a general property of complex I, which may be correlated with the metabolic characteristics of the organisms...
  85. ncbi Ion transport, membrane traffic and cellular volume control
    Christopher Grefen
    Laboratory of Plant Physiology and Biophysics, Institute of Molecular, Cellular and Systems Biology, University of Glasgow, Glasgow, UK
    Curr Opin Plant Biol 14:332-9. 2011
    Throughout their development, plants balance cell surface area and volume with ion transport and turgor...
  86. ncbi Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters
    Charles F Simard
    Nephrology Research Group, L Hôtel Dieu de Québec Research Institution, Department of Medicine, Faculty of Medicine, Laval University, Quebec, Quebec G1R 2J6, Canada
    J Biol Chem 282:18083-93. 2007
    ..Taken together, these findings could change our views on how CCCs operate or are regulated in animal cells by suggesting, in particular, that cation-Cl- cotransport achieves higher levels of functional diversity than foreseen...
  87. ncbi Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins
    Heather E Hallen
    Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
    Fungal Genet Biol 44:1146-56. 2007
    ..This is the first whole-genome analysis of differential transcript accumulation during sexual development in a filamentous fungus...
  88. pmc Role of epithelial HCO3⁻ transport in mucin secretion: lessons from cystic fibrosis
    Paul M Quinton
    Department of Pediatrics, Rady Children s Hospital, University of California San Diego School of Medicine, La Jolla, California 92093 0830, USA
    Am J Physiol Cell Physiol 299:C1222-33. 2010
    ....
  89. ncbi Regulation of K-Cl cotransport: from function to genes
    N C Adragna
    Department of Pharmacology, Wright State University, School of Medicine, Dayton, OH 45435 0002, USA
    J Membr Biol 201:109-37. 2004
    ..The K-Cl COT field is expected to expand further over the next decades, as new isoforms and/or regulatory pathways are discovered and its implication in health and disease is revealed...
  90. ncbi Electrostatic influence on ion transport through the alphaHL channel
    M Misakian
    Biotechnology Division, National Institute of Standards and Technology, ACSL 227 A251, Gaithersburg, MD 20899 8313, USA
    J Membr Biol 195:137-46. 2003
    ..The model demonstrates that rectification of monovalent ion flow through the alphaHL channel can be related to the asymmetry in the location of the ionizable amino acid side chains...
  91. ncbi Control of ion transport in mammalian airways by protease activated receptors type 2 (PAR-2)
    Karl Kunzelmann
    Institut fur Physiologie, Universitat Regensburg, Regensburg, Germany
    FASEB J 19:969-70. 2005
    ....
  92. ncbi Defective ENaC processing and function in tissue kallikrein-deficient mice
    Nicolas Picard
    INSERM U872 Centre de Recherche des Cordeliers, Universite Paris 5, Faculté de Médecine René Descartes, and Département de Physiologie, Hopital Necker Enfants Malades, F 75015, Paris, France
    J Biol Chem 283:4602-11. 2008
    ..The results demonstrate that TK, like several other proteases, can activate ENaC in the kidney and the colon...
  93. ncbi Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
    ..Inverted membrane vesicles catalysed respiratory Na+ uptake with NADH as electron donor. The sodium ion transport activity of vesicles from glycerol/fumarate-grown cells was 40 nmol mg-1 min-1 and was resistant to the ..
  94. ncbi Salvinorin A inhibits colonic transit and neurogenic ion transport in mice by activating kappa-opioid and cannabinoid receptors
    J Fichna
    Division of Gastroenterology, Department of Medicine, Snyder Institute of Infection, Immunity and Inflammation III, Alberta, Canada
    Neurogastroenterol Motil 21:1326-e128. 2009
    ..Colonic epithelial ion transport was evaluated using Ussing chambers...
  95. ncbi Potential role of abnormal ion transport in the pathogenesis of chronic sinusitis
    Kenji Dejima
    Department of Otorhinolaryngology, Kyoto Prefectural University of Medicine, Kyoto, Japan
    Arch Otolaryngol Head Neck Surg 132:1352-62. 2006
    ..To create well-differentiated cultures of normal and chronic sinusitis paranasal sinus epithelial cells and to compare their electrophysiologic properties...
  96. ncbi Upregulation of alveolar epithelial active Na+ transport is dependent on beta2-adrenergic receptor signaling
    Gokhan M Mutlu
    Division of Pulmonary and Critical Care Medicine, Northwestern University Feinberg School of Medicine, Chicago, Ill, USA
    Circ Res 94:1091-100. 2004
    ..These findings indicate that betaAR signaling may not be necessary for basal AFC, and that beta2AR is essential for the adaptive physiological response needed to clear excess fluid from the alveolar airspace of normal and injured lungs...
  97. pmc Human cystic fibrosis airway epithelia have reduced Cl- conductance but not increased Na+ conductance
    Omar A Itani
    Department of Internal Medicine, The Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 108:10260-5. 2011
    ..These results indicate that Na(+) conductance was not increased in these cultured CF tracheal/bronchial epithelia and point to loss of anion transport as key to airway epithelial dysfunction in CF...
  98. pmc Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination
    O Staub
    Hospital for Sick Children, Division of Respiratory Research, Toronto, Ontario, Canada
    EMBO J 16:6325-36. 1997
    ..Our results suggest that ENaC function is regulated by ubiquitination, and propose a paradigm for ubiquitination-mediated regulation of ion channels...
  99. ncbi Properties of BK(Ca) channels in oral keratinocytes
    D B Shieh
    Institute of Oral Medicine, National Cheng Kung University Medical College, No 1, University Road, Tainan 701, Taiwan
    J Dent Res 84:468-73. 2005
    ..The results demonstrate that the properties of BK(Ca) channels in normal human oral keratinocytes are similar to those described in other types of cells. Caffeic acid derivatives can also stimulate BK(Ca)-channel activity directly...
  100. ncbi Helix VIII of NhaA Na(+)/H(+) antiporter participates in the periplasmic cation passage and pH regulation of the antiporter
    Mohammad Diab
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    J Mol Biol 413:604-14. 2011
    ..Taken together, our results at physiological pH reveal that amino acid residues in transmembrane VIII contribute to the cation passage of NhaA and its pH regulation...
  101. pmc TMEM16A inhibitors reveal TMEM16A as a minor component of calcium-activated chloride channel conductance in airway and intestinal epithelial cells
    Wan Namkung
    Department of Medicine, University of California, San Francisco, California 94143, USA
    J Biol Chem 286:2365-74. 2011
    ..The small molecule inhibitors identified here permit pharmacological dissection of TMEM16A/CaCC function and are potential development candidates for drug therapy of hypertension, pain, diarrhea, and excessive mucus production...

Research Grants77

  1. Using Drosophila to understand WNK and SPAK/OSR1 regulation of SLC12 cotransporte
    AYLIN RACHEL RODAN; Fiscal Year: 2013
    ..with the long-term goal of becoming an independent academic investigator with a research focus on epithelial ion transport related to kidney physiology and diseases...
  2. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
    ..Of particular interest are immune suppression of inflammatory cytokines and related transcription factors compared to the regulation of ion transport pathways that control fluid homeostasis.
  3. Pilot Studies of the Effect of Aging on Mucociliary Clearance
    Lawrence E Ostrowski; Fiscal Year: 2013
    ..2c) To test the hypothesis that aged mice exhibit defects in the regulation of ion transport. The ion transport properties of the upper and lower airways wil be determined, as these are reflective of the ..
  4. Matrix Otopathology
    MICHAEL ANNE NMI GRATTON; Fiscal Year: 2011
    ..Following exposure, aspects of peripheral auditory function as well as the electrochemical and ion transport properties of the stria vascularis are measured to determine changes in the homeostasis of the stria and scala ..
  5. GILZ Regulation of ENaC
    David Pearce; Fiscal Year: 2012
    ..Moreover, it will shed new light on how specific regulation of ion transport can be achieved without unleashing undesirable effects on other processes, which is a central question in cell ..
  6. A Functional Role for the Circadian Clock Protein Per1 in the Regulation of ??ENa
    Michelle L Gumz; Fiscal Year: 2013
    ..Dr. Charles Wingo, a highly regarded nephrologist and expert in renal ion transport, will serve as the candidate's mentor...
  7. Prevention and Treatment of Chlorine Gas Induced Injury to the Pulmonary System
    Sadis Matalon; Fiscal Year: 2011
    ..as ascorbate, N-acetyl-cysteine (NAC), and deferoxamine, as well as agents that augment surfactant levels, ion transport and paracellular resistance (such as albuterol (a long acting b-agonist) and a recently described peptide ..
  8. Phospholemman and Cardiac Contracility
    Joseph Y Cheung; Fiscal Year: 2013
    ..PLM), the 1st member of the FXYD family of small integral membrane proteins involved in regulation of ion transport, modulates the function of both Na+-K+-ATPase and Na+/Ca2+ exchanger (NCX1) in the heart...
  9. Transcriptional Networks Regulating Luminal Environment in the Epididymis
    Ann Harris; Fiscal Year: 2013
    ..The CFTR channel makes an essential contribution to ion transport and the luminal environment in the epididymis and its loss causes obstruction and/or loss of the male genital ..
  10. ClC-3 ion transport modulates NADPH oxidase-dependent PMN priming by endotoxin
    Jessica G Moreland; Fiscal Year: 2012
    ....
  11. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    ..demonstrated that both WNK1 and WNK4 are present in a variety of polarized epithelia involved in chloride ion transport, such as kidney, clonic crypts, sweat ducts, pancreatic ducts, biliary ducts, epididymis and the ependyma of ..
  12. Novel STIM1-dependent cyclic AMP signaling pathway in colonic epithelial function
    Aldebaran M Hofer; Fiscal Year: 2013
    ..Finally, we propose to investigate the extent to which store depletion-dependent cAMP generation regulates the ion transport properties of the native epithelium of the rat large intestine (Specific Aim #4)...
  13. Investigating SloR virulence gene metalloregulation in S. mutans.
    Grace A Spatafora; Fiscal Year: 2013
    ..During the previous grant term, we confirmed SloR-dependent repression of S. mutans metal ion transport genes and virulence factors when sustainable levels of manganese are achieved (such as during a mealtime), and ..
  14. Molecular Mechanisms of Intestinal Metal Ion Transport During Iron Deficiency
    James F Collins; Fiscal Year: 2013
    ..Overall, this project will advance the field of iron biology by revealing new mechanistic details of iron transport and may provide opportunities to develop molecular approaches to modulate iron absorption. ..
  15. Mining open chromatin to define molecular mechanisms of CF modifier genes
    WANDA K O'NEAL; Fiscal Year: 2013
    ..CF is characterized by abnormal ion transport, reduced mucociliary clearance, chronic bacterial infection, inflammatory airway disease with mucus hyper/..
  16. Investigating SloR virulence gene metalloregulation in S. mutans.
    Grace Spatafora; Fiscal Year: 2009
    ..During the previous grant term, we confirmed SloR-dependent repression of S. mutans metal ion transport genes and virulence factors when sustainable levels of manganese are achieved (such as during a mealtime), and ..
  17. PAF Receptor Trafficking and Function in Epithelial Cells
    Tamas Jilling; Fiscal Year: 2010
    ..in cultured intestinal epithelial cells and regulates such diverse cellular functions as gene expression, ion transport, intracellular pH, and cell death...
  18. RENAL FUNCTION AND DISEASE
    Bruce A Stanton; Fiscal Year: 2013
    ..S. Areas in which training is offered include ion transport and its pharmacological and physiological regulation, effects of mineralocorticoids on ion transport, ..
  19. CHLORIDE CHANNELS IN LUNG DEVELOPMENT
    Pamela L Zeitlin; Fiscal Year: 2012
    ..Aim 2: To determine whether ClC-2 and ENaC interact through Cl- current to modulate ion transport in the CF mouse lung. The hypothesis is that chloride conductance through ClC-2 will inhibit ENaC function...
  20. Novel Ca receptor signaling pathways for control of renal ion transport
    Richard Tyler Miller; Fiscal Year: 2012
    ..The overall hypotheses of this application is that the CaR reduces distal nephron ion transport by activating WNK kinases that reduce cell surface expression and activity of Kir4...
  21. Inhibition of chloride secretion by cigarette smoke
    JAMES LEVIN KREINDLER; Fiscal Year: 2010
    ..fluorescence microscopy, and protein biochemical techniques to study the effect of cigarette smoke on ion transport of human bronchial epithelial cells...
  22. Role of H,K-ATPase in the Action of Mineralocorticoids
    Charles S Wingo; Fiscal Year: 2013
    ..Mineralocorticoids affect distal nephron and collecting duct (CD) ion transport to achieve these effects...
  23. Potassium Homeostasis in the Inner Ear
    Ebenezer N Yamoah; Fiscal Year: 2013
    ..of the specific functions of individual K+ and Cl- channels, as well as the different cell types in the walls of the cochlea, and how they work together to mediate EP and trans-epithelial ion transport processes in vivo.
  24. Functional Anatomic Imaging of CF Patients with Early Lung Disease Using microOCT
    Guillermo J Tearney; Fiscal Year: 2013
    ..key hypotheses and resolve controversies in the field regarding the interrelationships between the CFTR ion transport defect and the regulations of ASL/PCL depth, ciliary function, and the physical properties of mucus, and the ..
  25. Na-K-Cl cotransporter in Glioblastoma Multiforme
    Dandan Sun; Fiscal Year: 2013
    ..Our preliminary data illustrate that NKCC1 is the most important ion transport mechanism in regulating Cl-i and RVI in GBM cancer cells...
  26. Neuroendocrine-modulated epithelial HCO3- transport
    Bruce D Schultz; Fiscal Year: 2013
    ..We established protocols to study ion transport in the intact porcine vas deferens, in freshly isolated human and porcine tissues, in primary cell cultures, ..
  27. Physiologic role of the gamma epithelial sodium channel subunit in the kidney
    Donald E Kohan; Fiscal Year: 2010
    ..the role of ? ENaC in the regulation of blood pressure, renal electrolyte excretion, and collecting duct ion transport. 2. Generation and characterization of mice with renal-specific reconstitution of wild-type or mutant ? ENaC...
  28. KINASES IN ION COTRANSPORTER FUNCTION
    Eric J Delpire; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Protein kinases are critical in regulating the activity of ion transport mechanisms involved in salt and fluid reabsorption in the kidney, control of blood pressure, and modulation of inhibitory ..
  29. The Role of Uromodulin in the Regulation of the Renal Calcium Channel TRPV5
    MATTHIAS TILMANN FLORIAN WOLF; Fiscal Year: 2013
    ..with the long-term goal of becoming an independent academic investigator with a research focus on epithelial ion transport related to kidney physiology and diseases...
  30. Na+-K+-CI- Cotransporter in Cerebral Ischemia
    Dandan Sun; Fiscal Year: 2012
    ..The long-term goal of the research is to understand the role of ion transport proteins in disruption of ion homeostasis following ischemia and to determine whether these ion transport ..
  31. Structure and Function of Sodium-Calcium Exchangers
    DINESH A YERNOOL; Fiscal Year: 2013
    ..Wild-type ion transport kinetics will be determined and inhibitors of transport identified. 3. Structure...
  32. Targeting compartmentalized phosphodiesterases to regulate CFTR function
    WITO H RICHTER; Fiscal Year: 2013
    ..However, stimulating CFTR-dependent ion transport in CF model systems by increasing global cellular cAMP to supra-physiological levels with a combination of ..
  33. Perivascular macrophages endothelial interactions at the blood labyrinth barrier
    Xiao Rui Shi; Fiscal Year: 2013
    ..The blood-labyrinth barrier (BLB) in the stria vascularis of the cochlea maintains endocochlear potential, ion transport, and fluid balance in the inner ear...
  34. Ion transport and motility in larval mosquito stomach
    David F Moffett; Fiscal Year: 2010
    ..The processes of ion transport, their regulation and their coordination with peristalsis in the stomach of larval mosquitoes are poorly ..
  35. Molecular Physiology of Polycystin-2
    Horacio F Cantiello; Fiscal Year: 2010
    ..The pathogenesis of ADPKD entails dysfunctional ion transport and/or regulatory mechanisms that impair ion transport, in particular calcium, mediated by PC2 in renal ..
  36. Genetic Models for Disorders of Acid-Base Transport
    GARY EDWARD SHULL; Fiscal Year: 2013
    ..is to understand the functions and coupling modes of bicarbonate and proton transporters in transepithelial ion transport. HCO 3 and H are generated from H2 O and CO2 via carbonic anhydrase activity and coupled to transport of other ..
  37. Mechanisms and Relevance of Sodium Transport Regulation by AMPK
    Kenneth R Hallows; Fiscal Year: 2013
    ..other important epithelial transport proteins, which appears to provide a sensitive coupling mechanism between ion transport and cellular metabolic status and helps conserve cellular energy under conditions of metabolic depletion ..
  38. Membrane Trafficking of Renal Ion Transport Proteins in Potassium Homeostasis
    Chou Long Huang; Fiscal Year: 2013
    ..These studies will provide important insights to the in vivo role of WNK1 in renal Na+ and K+ transport in the normal physiology and in diseased states. ..
  39. Anesthetic Sites in Transmembrance Peptides by NMR
    Pei Tang; Fiscal Year: 2012
    ..and presence of anesthetics (halothane and isoflurane);and (3) to correlate channel global dynamics with ion transport in the presence and absence of anesthetics (a) by determining the dynamics of channel mutants having altered ..
  40. MODEL SYNTHETIC CHANNEL ASSEMBLIES
    John M Tomich; Fiscal Year: 2013
    ..our de novopores tolerate them well with the net anion flux controlled by the natural regulation of counter-ion transport. In the Aims we propose to modulate pore-exposed and peptide-peptide interfacial residues that dictate the ..
  41. Novel Mitochondrial Ion Transporters
    BRIAN O'ROURKE; Fiscal Year: 2013
    ..and intact hearts, a major limitation has been the lack of molecular information about the proteins mediating ion transport in the inner membrane...
  42. Elucidating the molecular function of Hph1 in regulating metal ion homeostasis
    Francisco Pina; Fiscal Year: 2009
    ..Many proteins that facilitate ion transport, storage, and utilization in the cell are highly conserved through evolution...
  43. Methods & Noninvasive PK Study to Improve Iontophoresis
    S Kevin Li; Fiscal Year: 2010
    ..significant improvement of ocular iontophoretic drug delivery from the standpoint of the physical chemistry of ion transport and engineering...
  44. Electron Microscopy of P-type Ion Pumps
    David L Stokes; Fiscal Year: 2010
    ..The P1a subfamily is characterized by separate subunits for ATP hydrolysis and ion transport and appears to be directly descended from a primordial ancestor...
  45. The Functional Role of the PXL Domain of SGK1 in Epithelial Sodium Transport
    Alan C Pao; Fiscal Year: 2010
    ..Dr. David Pearce, who is a leader in the field of hormone regulation of ion transport, will mentor the candidate's scientific development. Moreover, Dr...
  46. mTOR signaling in Polycystic Kidney Disease
    Atif Kidwai; Fiscal Year: 2011
    ..primarily in protein synthesis and proliferation, while mTORC2 functions in the regulation of metabolism, ion transport, cytoskeletal rearrangements and apoptosis, which are all altered in PKD (4)...
  47. The role of NDRG1 in collecting duct function.
    ANIKO NARAY FEJES TOTH; Fiscal Year: 2010
    ..mice as well as in isolated cultured kidney cells to study the role of one particular protein, called NDRG1 in ion transport by the kidney...
  48. Chloride secretagogues for acquired CFTR dysfunction in chronic rhinosinusitis
    Bradford Alan Woodworth; Fiscal Year: 2013
    ..Specific Aim 2 will test the hypothesis that Cl- secretagogues can offset acquired defects in CFTR-mediated ion transport. Compounds of this class, including VX-770, UCCF-152, and bioflavonoids have received considerable recent ..
  49. Redox regulation of alveolar fluid balance
    My N Helms; Fiscal Year: 2012
    ..It has been established that net ion transport through amiloride-sensitive epithelial sodium channels (ENaC), located on the apical surface of alveolar ..
  50. REGULATION OF THE SODIUM CHLORIDE COTRANSPORTER
    Robert S Hoover; Fiscal Year: 2010
    ..independent principal investigator with a basic science laboratory examining the molecular physiology of renal ion transport. After completing 6 years of training with Dr...
  51. Intestinal Epithelia Ammonium Transport
    Roger T Worrell; Fiscal Year: 2012
    ..b>Ion transport in the colon has been a target of particular interest...
  52. LIGHT-DRIVEN ION TRANSPORT IN BACTERIAL RHODOPSINS
    Janos K Lanyi; Fiscal Year: 2013
    ..pumps that deviate from those in a model system employed earlier to generate general principles for active ion transport. The aims of this proposal focus on the functional implications of our recently reported x-ray diffraction ..
  53. Interactions of engineered nanomaterials with lung alveolar epithelium
    EDWARD DAVID CRANDALL; Fiscal Year: 2010
    ..water-loving)) injure and/or are translocated into and/or across alveolar epithelium (where gas exchange and ion transport occur in the distal portion of the lung) are not well understood...
  54. Active transmembrane water cycling kinetics: A Cellular Metabolic 1H MR Biomarker
    JAMES ALVIN BALSCHI; Fiscal Year: 2013
    ..have found that steady-state water exchange correlates with the activity of the yeast cellular membrane major ion transport protein, the P-type H+-ATPase...
  55. CWRU NEPHROLOGY TRAINING GRANT
    John R Sedor; Fiscal Year: 2013
    ..interests of the primary faculty include glomerular development and disease, epithelial cell biology and ion transport, genetic epidemiology, health services research and clinical trials...
  56. BLOOD-BRAIN BARRIER ION TRANSPORT IN CEREBRAL ISCHEMIA
    MARTHA E O'DONNELL; Fiscal Year: 2013
    ..We will also assess the efficacy of NKCC, NHE and NBC inhibitors for reduction of edema when administered after the onset of ischemia. ..
  57. Specifying Control Over Ion Balance and Glucose Homeostasis Through mTORC2
    CATHERINE GLEASON; Fiscal Year: 2013
    ..physiology and signal transduction with additional training in the cellular and whole animal study of ion transport processes to address the mechanistic basis for mTORC2 specificity towards SGK1 vs...
  58. MItochondrial Dysfunction in Cardiac Hypertrophy and Failure
    Jennifer E Van Eyk; Fiscal Year: 2013
    ..biology, redox modulation, proteomics, and computational biology to investigate how the remodeling of ion transport pathways and mitochondrial proteins contribute to maladaptive responses in a pressure-overload model of ..
  59. Parenteral Nutrition: Intestinal Metabolism/Adaptation
    Denise Ney; Fiscal Year: 2009
    ..Intestinal adaptive growth will be assessed by changes in cellularity, structure and ion transport function...
  60. Role of the Na K ATPase Beta 1 subunit in alveolar epithelial integrity
    Olga Vagin; Fiscal Year: 2013
    ..The active transepithelial Na+ ion transport performed in part by the alveolar epithelial Na,K-ATPase is critical for lung edema clearance...
  61. RESEARCH TRAINING IN NEPHROLOGY
    Martin R Pollak; Fiscal Year: 2013
    ..Current research areas include acute renal failure, mechanisms of cell injury and cell metabolism, ion transport and water excretion, cellular transduction mechanisms, mechanisms of muscle wasting and intracellular protein ..
  62. Integrative Membrane Physiology in the Post-Genome Era
    Jerod S Denton; Fiscal Year: 2012
    ..A highly ambitious program comprising a veritable "who's who" in the ion transport field has been carefully developed for the SGP 2012 symposium...
  63. CYTOSOLIC REGULATION OF INNER EAR ION TRANSPORT
    A PHILINE WANGEMANN; Fiscal Year: 2007
    Cytosolic Regulation of Inner Ear Ion Transport Defects in potassium cycling, gap junction-mediated intercellular communication and cochlear metabolism are re- sponsible for the ovelwhelming majority of hearing impairments This proposal ..
  64. EFFECTS OF ANTICONVULSANTS ON GLIAL ION TRANSPORT
    Steve White; Fiscal Year: 1991
    ..Thus, through an increased understanding of the mechanisms of the currently available anticonvulsant drugs and the processes underlying seizure disorders, drugs with greater selectively and fewer adverse effects can be developed...
  65. CALMODULIN KINASE II AND ION TRANSPORT IN THE KIDNEY
    Rochelle Hanley; Fiscal Year: 1992
    ..the effects of PKA and PKC, should provide new information on the regulatory role of CaM-KII in the control of ion transport in the renal proximal tubule...
  66. THE GASTRIC ACID PUMP AS A TARGET FOR ULCER TREATMENT
    George Sachs; Fiscal Year: 2009
    ..has a very similar overall structure and also uses carboxylic acid clusters in the membrane domain as the ion transport- binding and export-import sites as does the Na, K+ ATPase that is 75% homologous to the H+,K+ ATPase...
  67. CF TS TRANSFORMANTS--BIOCHEMICAL AND GENETIC ANALYSIS
    Dieter Gruenert; Fiscal Year: 1992
    ..will initially be assayed for their epithelial origin, transformation- specific antigens, and defective Cl ion transport. Individual colonies will be analyzed at the permissive (33 degrees C) and nonpermissive (40 degrees C) ..
  68. ESTROGEN AND THE MALE REPRODUCTIVE TRACT
    Rex Hess; Fiscal Year: 2000
    ..that estrogen regulates fluid reabsorption in efferent ductule through direct or indirect alterations in ion transport. This hypothesis will be tested using the following specific aims: 1...
  69. REGULATION OF VOCAL FOLD HYDRATION AND PHONATION
    Kimberly Fisher; Fiscal Year: 2003
    ..The hypothesis is based on our novel observations about mechanisms of epithelial water/ion transport in the vocal folds. Kimberly Fisher, Ph.D...
  70. CALCIUM SIGNALING IN AIRWAY EPITHELIA AND SMOOTH MUSCLE
    Michael Sanderson; Fiscal Year: 2002
    ..These cells perform muco-ciliary clearance and ion transport to expel inhaled contaminants and prevent surface dehydration...
  71. CL-/HCO3- EXCHANGE IN CYSTIC FIBROSIS SWEAT GLANDS
    Paul Quinton; Fiscal Year: 2001
    ..Defining HCO3 ion-transport and its relevance to CFTR function should lend much to explaining, and hopefully, ameliorating these problems. ..
  72. CORTICOSTEROID CONTROL OF COLONIC CATION TRANSPORT
    CHRISTINE BASTL; Fiscal Year: 1990
    Glucocorticoids potently regulate ion transport in intestinal epithelia...
  73. INHIBITION OF ION TRANSPORT IN DEOXYGENATED SICKLE CELLS
    Clinton Joiner; Fiscal Year: 1999
    ..containing Hemoglobin S(SS RBC) increases membrane permeability to Na, K, and Ca, which perturbs a number of ion transport mechanisms and leads to cellular dehydration...
  74. MECHANISM OF ACTIVE ION TRANSPORT--STRUCTURAL STUDIES
    J Blasie; Fiscal Year: 1999
    DESCRIPTION: The goal of this project is to determine the actual mechanism of active (energy dependent) ion transport across biological membranes to at least the submolecular level...
  75. ADENOSINE RECEPTORS AND ION TRANSPORT IN EPITHELIA
    John Forrest; Fiscal Year: 2003
    The long term goal of this project is to understand the mechanisms by which adenosine receptors regulate ion transport. In ion transporting epithelia, adenosine plays a central role is a regulatory link between cellular energy demand and ..
  76. MECHANISMS OF CL TRANSPORT IN EPITHELIAL CELLS
    Thomas Biber; Fiscal Year: 1992
    The overall goal of this project is to gain a better understanding of the processes that underly and control ion transport in epithelial cells of Na transporting tissues...
  77. CHLORIDE CHANNELS WITH PHOTOAFFINITY PROBES
    Bruce Branchini; Fiscal Year: 1990
    ..goals of the proposed research are to develop reagents: (1) to identify a component(s) essential to chloride ion transport in airway epithelium, which is defective in cystic fibrosis (CF); and (2) to contribute to the basic ..