aerobiosis

Summary

Summary: Life or metabolic reactions occurring in an environment containing oxygen.

Top Publications

  1. ncbi Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
    G Unden
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Biochim Biophys Acta 1320:217-34. 1997
  2. pmc Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function
    Yisheng Kang
    Department of Genetics, 425 Henry Mall, University of Wisconsin, Madison, WI 53706, USA
    J Bacteriol 187:1135-60. 2005
  3. ncbi Cardiovascular risk factors emerge after artificial selection for low aerobic capacity
    Ulrik Wisløff
    Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Olav Kyrres gt 3, 7489 Trondheim, Norway
    Science 307:418-20. 2005
  4. ncbi Effect of hydrogen peroxide production and the Fenton reaction on membrane composition of Streptococcus pneumoniae
    Stella Pesakhov
    Pediatric Infectious Disease Unit, Soroka University Medical Center, Faculty of Health Sciences P O Box 151, Beer Sheva 84101, Israel
    Biochim Biophys Acta 1768:590-7. 2007
  5. pmc Homeostatic adjustment and metabolic remodeling in glucose-limited yeast cultures
    Matthew J Brauer
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94122, USA
    Mol Biol Cell 16:2503-17. 2005
  6. ncbi The NADH oxidase of Streptococcus pneumoniae: its involvement in competence and virulence
    I Auzat
    Laboratoire d Enzymologie et de Biochimie structurale du Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    Mol Microbiol 34:1018-28. 1999
  7. pmc Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli
    Svetlana Alexeeva
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Bacteriol 184:1402-6. 2002
  8. pmc Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations
    Matthew D Rolfe
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK
    Open Biol 2:120091. 2012
  9. pmc Nitric oxide-induced bacteriostasis and modification of iron-sulphur proteins in Escherichia coli
    Binbin Ren
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Microbiol 70:953-64. 2008
  10. pmc Heterotrophic archaea contribute to carbon cycling in low-pH, suboxic biofilm communities
    Nicholas B Justice
    University of California Berkeley, Berkeley, California, USA
    Appl Environ Microbiol 78:8321-30. 2012

Detail Information

Publications272 found, 100 shown here

  1. ncbi Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
    G Unden
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Biochim Biophys Acta 1320:217-34. 1997
    ..Reductive activation could be achieved by cellular reductants in the absence of O2. In addition, O2 may cause destruction and loss of the FeS cluster. It is not known whether this process is required for regulation of FNR function...
  2. pmc Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function
    Yisheng Kang
    Department of Genetics, 425 Henry Mall, University of Wisconsin, Madison, WI 53706, USA
    J Bacteriol 187:1135-60. 2005
    ..We suggest that E. coli MG1655 has a larger metabolic potential under anaerobic conditions than has been previously recognized...
  3. ncbi Cardiovascular risk factors emerge after artificial selection for low aerobic capacity
    Ulrik Wisløff
    Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Olav Kyrres gt 3, 7489 Trondheim, Norway
    Science 307:418-20. 2005
    ..Impairment of mitochondrial function may link reduced fitness to cardiovascular and metabolic disease...
  4. ncbi Effect of hydrogen peroxide production and the Fenton reaction on membrane composition of Streptococcus pneumoniae
    Stella Pesakhov
    Pediatric Infectious Disease Unit, Soroka University Medical Center, Faculty of Health Sciences P O Box 151, Beer Sheva 84101, Israel
    Biochim Biophys Acta 1768:590-7. 2007
    ..We suggest that endogenous ROS play an important regulatory role in membrane adaptation, allowing the survival of this anaerobic organism at aerobic environments of the host...
  5. pmc Homeostatic adjustment and metabolic remodeling in glucose-limited yeast cultures
    Matthew J Brauer
    Department of Genetics, Stanford University School of Medicine, Stanford, CA 94122, USA
    Mol Biol Cell 16:2503-17. 2005
    ..These results suggest that some aspect of actual starvation, possibly a component of the stress response, may be required for triggering the metabolic remodeling associated with the diauxic shift...
  6. ncbi The NADH oxidase of Streptococcus pneumoniae: its involvement in competence and virulence
    I Auzat
    Laboratoire d Enzymologie et de Biochimie structurale du Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    Mol Microbiol 34:1018-28. 1999
    ..This work establishes, for the first time, the role of O2 in the regulation of pneumococcal transformability and virulence...
  7. pmc Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli
    Svetlana Alexeeva
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Bacteriol 184:1402-6. 2002
    ..Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based on a calibration with reference to the minimal oxygen supply rate needed ..
  8. pmc Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations
    Matthew D Rolfe
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK
    Open Biol 2:120091. 2012
    ..Thus, this approach illustrates how systematic examination of regulatory responses in stable and dynamic environments yields new mechanistic insights into adaptive processes...
  9. pmc Nitric oxide-induced bacteriostasis and modification of iron-sulphur proteins in Escherichia coli
    Binbin Ren
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Mol Microbiol 70:953-64. 2008
    ..coli cells. The results suggest that cellular deficiency to repair the NO-modified iron-sulphur proteins may directly contribute to the NO-induced bacteriostasis under anaerobic conditions...
  10. pmc Heterotrophic archaea contribute to carbon cycling in low-pH, suboxic biofilm communities
    Nicholas B Justice
    University of California Berkeley, Berkeley, California, USA
    Appl Environ Microbiol 78:8321-30. 2012
    ..These findings indicate dominance of acidophilic archaea in degrading biofilms and suggest that they play roles in anaerobic nutrient cycling at low pH...
  11. ncbi Oxygen and the spatial structure of microbial communities
    Tom Fenchel
    Marine Biological Laboratory, University of Copenhagen, Strandpromenaden 5, DK 3000 Helsingør, Denmark
    Biol Rev Camb Philos Soc 83:553-69. 2008
    ..Thus the oxygenated sediments surrounding ventilated animal burrows provide a special habitat for metazoan meiofauna as well as unicellular organisms...
  12. ncbi Protein domain structure uncovers the origin of aerobic metabolism and the rise of planetary oxygen
    Kyung Mo Kim
    Evolutionary Bioinformatics Laboratory, Department of Crop Sciences, University of Illinois, Urbana, IL 61801, USA
    Structure 20:67-76. 2012
    ..The evolutionary patterns we uncover in molecules and chemistries provide strong support for the coevolution of biochemistry and geochemistry...
  13. pmc Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients
    Dieter Worlitzsch
    Institute of General and Environmental Hygiene, University of Tubingen, Tubingen, Germany
    J Clin Invest 109:317-25. 2002
    ..These studies indicate that novel therapies for CF include removal of hypoxic mucus plaques and antibiotics effective against P. aeruginosa adapted to anaerobic environments...
  14. ncbi Assessing diversity and biogeography of aerobic anoxygenic phototrophic bacteria in surface waters of the Atlantic and Pacific Oceans using the Global Ocean Sampling expedition metagenomes
    Natalya Yutin
    Biology Department, Technion Israel Institute of Technology, Haifa 32000, Israel
    Environ Microbiol 9:1464-75. 2007
    ..Our results support the notion that marine AAnP populations are complex and dynamic, and compose an important fraction of bacterioplankton assemblages in certain oceanic areas...
  15. ncbi Aerobic regulation of cytochrome d oxidase (cydAB) operon expression in Escherichia coli: roles of Fnr and ArcA in repression and activation
    P A Cotter
    Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles 90095 1489, USA
    Mol Microbiol 25:605-15. 1997
    ..Based on the results of the in vitro and in vivo studies, a working model for ArcA activation and Fnr repression of cydAB transcription is proposed...
  16. pmc Biophysical characterization of the iron in mitochondria from Atm1p-depleted Saccharomyces cerevisiae
    Ren Miao
    Department of Chemistry, Texas A and M University, College Station, Texas 77843 3255, USA
    Biochemistry 48:9556-68. 2009
    ..Reactive oxygen species generated under these conditions might degrade iron-sulfur clusters and lower heme levels in the organelle...
  17. pmc Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    J Bacteriol 189:1845-55. 2007
    ....
  18. pmc Aerobic growth at nanomolar oxygen concentrations
    Daniel A Stolper
    Nordic Center for Earth Evolution and Institute of Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
    Proc Natl Acad Sci U S A 107:18755-60. 2010
    ..Our study expands both the environmental range and temporal history of aerobic organisms...
  19. pmc Time-dependent proteome alterations under osmotic stress during aerobic and anaerobic growth in Escherichia coli
    Arnim Weber
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Bereich Mikrobiologie, Maria Ward Str 1a, D 80638 München, Germany
    J Bacteriol 188:7165-75. 2006
    ..Only a few proteins were found to be down-regulated upon osmotic stress...
  20. pmc Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli
    C P Tseng
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    J Bacteriol 178:1094-8. 1996
    ..These two transcriptional regulators coordinate the hierarchial control of respiratory pathway gene expression in E. coli to ensure the optimal use of oxygen in the cell environment...
  21. pmc Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin
    D R Sherman
    Department of Pathobiology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 98:7534-9. 2001
    ..When we interrupted expression of this operon by targeted disruption of the upstream gene Rv3134c, the hypoxic regulation of acr was eliminated. These results suggest a possible role for Rv3132c/3133c/3134c in mycobacterial latency...
  22. pmc Aerobic fermentation of D-glucose by an evolved cytochrome oxidase-deficient Escherichia coli strain
    Vasiliy A Portnoy
    Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0412, USA
    Appl Environ Microbiol 74:7561-9. 2008
    ..E. coli strains produced in this study showed the ability to produce lactate as a fermentation product from glucose and to undergo mixed-acid fermentation during aerobic growth...
  23. ncbi The Campylobacter jejuni PEB1a adhesin is an aspartate/glutamate-binding protein of an ABC transporter essential for microaerobic growth on dicarboxylic amino acids
    Maria del Rocio Leon-Kempis
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 60:1262-75. 2006
    ..It is concluded that in addition to the established role of PEB1a as an adhesin, the PEB1 transport system plays a key role in the utilization of aspartate and glutamate, which may be important in vivo carbon sources for this pathogen...
  24. pmc Isolation of an oxygen-sensitive FNR protein of Escherichia coli: interaction at activator and repressor sites of FNR-controlled genes
    S B Melville
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    Proc Natl Acad Sci U S A 93:1226-31. 1996
    ..A model whereby the FNR protein exists in the cell as a monomer that assembles on the DNA under anaerobic conditions to form a dimer is discussed...
  25. ncbi SAR11 marine bacteria require exogenous reduced sulphur for growth
    H James Tripp
    Department of Microbiology, 220 Nash Hall, Oregon State University, Corvallis, Oregon 97331, USA
    Nature 452:741-4. 2008
    ..P. ubique' relies exclusively on reduced sulphur compounds that originate from other plankton...
  26. ncbi Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate
    Henry Lin
    Department of Bioengineering, Rice University, Houston, TX 77005, USA
    Biotechnol Bioeng 89:148-56. 2005
    ..Nevertheless, this aerobic succinate production system provides the first platform for enhancing succinate production aerobically in E. coli based on the creation of a new aerobic central metabolic network...
  27. pmc Effects of oxygen on virulence traits of Streptococcus mutans
    Sang Joon Ahn
    Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL 32610, USA
    J Bacteriol 189:8519-27. 2007
    ..mutans to modulate virulence gene expression and exopolysaccharide production in response to changes in oxygen availability...
  28. ncbi Stepwise reduction of the culture redox potential allows the analysis of microaerobic metabolism and photosynthetic membrane synthesis in Rhodospirillum rubrum
    Lisa Carius
    Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstr 1, Magdeburg, Sachsen Anhalt 39106, Germany
    Biotechnol Bioeng 110:573-85. 2013
    ..Furthermore, the introduced microaerobic cultivation setup should be generally applicable for any microbial system of interest which can be cultivated in common stirred-tank bioreactors...
  29. pmc High-level production of the industrial product lycopene by the photosynthetic bacterium Rhodospirillum rubrum
    Guo shu Wang
    Department of Bioenergetics, Institute of Biology, University of Stuttgart, Stuttgart, Germany
    Appl Environ Microbiol 78:7205-15. 2012
    ..This study provides the first genetic proof that the R. rubrum CrtI produces lycopene exclusively as an end product...
  30. pmc Involvement of pyruvate oxidase activity and acetate production in the survival of Lactobacillus plantarum during the stationary phase of aerobic growth
    Philippe Goffin
    Unité de Génétique, Institut des Sciences de la Vie, Universite Catholique de Louvain, Croix du Sud 5, B 1348 Louvain la Neuve, Belgium
    Appl Environ Microbiol 72:7933-40. 2006
    ..Notably, survival during the stationary phase appeared to be dramatically improved in the poxB poxF double mutant...
  31. pmc Cytochrome c-mediated formation of S-nitrosothiol in cells
    Katarzyna A Broniowska
    Department of Biophysics and Redox Biology Program, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Biochem J 442:191-7. 2012
    ..Taken together, these data point to cytochrome c as a biological mediator of protein S-nitrosation in cells. This is the most efficient and concerted mechanism of S-nitrosothiol formation reported so far...
  32. ncbi Insufficient uracil supply in fully aerobic chemostat cultures of Saccharomyces cerevisiae leads to respiro-fermentative metabolism and double nutrient-limitation
    Thiago Olitta Basso
    Department of Chemical Engineering, University of Sao Paulo, C P 61548, 05424 970, Sao Paulo, SP, Brazil
    Biotechnol Lett 32:973-7. 2010
    ....
  33. ncbi Understanding and harnessing the microaerobic metabolism of glycerol in Escherichia coli
    Guyton Durnin
    Department of Chemical and Biomolecular Engineering, Rice University, 6100 Main Street, MS 362, P O Box 1892, Houston, Texas 77251 1892, USA
    Biotechnol Bioeng 103:148-61. 2009
    ..Engineered strains efficiently produced ethanol and hydrogen and ethanol and formate from glycerol in a minimal medium without rich supplements...
  34. pmc Metabolism of 2-chloro-4-nitroaniline via novel aerobic degradation pathway by Rhodococcus sp. strain MB-P1
    Fazlurrahman Khan
    Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh, India
    PLoS ONE 8:e62178. 2013
    ..This is the first report showing 2-C-4-NA degradation and elucidation of corresponding metabolic pathway by an aerobic bacterium...
  35. ncbi Identity, abundance and ecophysiology of filamentous Chloroflexi species present in activated sludge treatment plants
    Caroline Kragelund
    Section of Environmental Engineering, Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark
    FEMS Microbiol Ecol 59:671-82. 2007
    ..These results are generally supported by physiological studies of two new isolates. Based on the results obtained in this study, the potential role of filamentous Chloroflexi species in activated sludge is discussed...
  36. ncbi Microbial characterisation of polyhydroxyalkanoates storing populations selected under different operating conditions using a cell-sorting RT-PCR approach
    Paulo C Lemos
    REQUIMTE CQFB, Chemistry Department, FCT UNL, 2829 516, Caparica, Portugal
    Appl Microbiol Biotechnol 78:351-60. 2008
    ..Azoarcus cells were found in all the analysed systems (3.9 +/- 0.3, 23.3 +/- 1.5 and 45.9 +/- 1.5 for P, A and A1, respectively), while Paracoccus was scarcely present...
  37. ncbi Distribution and genetic diversity of functional microorganisms in different CANON reactors
    Tao Liu
    State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
    Bioresour Technol 123:574-80. 2012
    ..Meanwhile, this study has demonstrated the feasibility of starting up CANON by inoculating conventional activated sludge in low ammonium content at ambient temperature...
  38. ncbi Aerobic mineralization of nitroguanidine by Variovorax strain VC1 isolated from soil
    Nancy N Perreault
    Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2, Canada
    Environ Sci Technol 46:6035-40. 2012
    ..This first description of NQ mineralization by a bacterial isolate demonstrates the potential for efficient microbial remediation of NQ in soil...
  39. ncbi FNR and its role in oxygen-regulated gene expression in Escherichia coli
    S Spiro
    Department of Molecular Biology and Biotechnology, University of Sheffield, U K
    FEMS Microbiol Rev 6:399-428. 1990
    ..The relationships between FNR and other oxygen-related systems in E. coli are discussed, as well as parallel systems in other organisms.(ABSTRACT TRUNCATED AT 400 WORDS)..
  40. pmc Reprogramming of Escherichia coli K-12 metabolism during the initial phase of transition from an anaerobic to a micro-aerobic environment
    Eleanor W Trotter
    Department of Molecular Biology and Biotechnology, The Krebs Institute, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 6:e25501. 2011
    ..However, the dynamics of adaptation when bacteria experience changes in O₂ availability are understudied. The model bacterium and facultative anaerobe Escherichia coli K-12 provides an ideal system for exploring this process...
  41. ncbi Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: a study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile
    Henry Lin
    Department of Bioengineering, Rice University, Houston, Texas, USA
    Metab Eng 7:337-52. 2005
    ..Strain HL27659k was the only strain without pyruvate accumulation, and its acetate production was the least among all the mutant strains examined...
  42. pmc Pathway and evolutionary implications of diphenylamine biodegradation by Burkholderia sp. strain JS667
    Kwanghee A Shin
    School of Civil and Environmental Engineering, Georgia Institute of Technology, 311 Ferst Dr, Atlanta, GA 30332 0512, USA
    Appl Environ Microbiol 75:2694-704. 2009
    ..Both the sequence similarity and the gene organization suggest that the DPA degradation pathway evolved recently by the recruitment of two gene clusters that encode the DPA dioxygenase and aniline degradation pathway...
  43. ncbi Quinones as the redox signal for the arc two-component system of bacteria
    D Georgellis
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Science 292:2314-6. 2001
    ..of quinone electron carriers act as direct negative signals that inhibit autophosphorylation of ArcB during aerobiosis. Thus, the Arc signal transduction system provides a link between the electron transport chain and gene ..
  44. ncbi Characterization of E. coli MG1655 and frdA and sdhC mutants at various aerobiosis levels
    S Steinsiek
    MPI für Dynamik Komplexer Technischer Systeme, Germany
    J Biotechnol 154:35-45. 2011
    ..mutants lacking succinate dehydrogenase (SDH) and fumarate reductase (FRD) activities was analyzed at different aerobiosis levels. Growth rate and cell yield were very similar to the parent strain...
  45. ncbi Effect of oxygen limitation on the in vitro activity of levofloxacin and other antibiotics administered by the aerosol route against Pseudomonas aeruginosa from cystic fibrosis patients
    Paula King
    Mpex Pharmaceuticals, Inc, San Diego, CA 92121 1309, USA
    Diagn Microbiol Infect Dis 66:181-6. 2010
    ..In contrast, levofloxacin maintains activity against P. aeruginosa under anaerobic or hypoxic conditions similar to those found in CF microaerophilic environments...
  46. pmc Initial reactions in the biodegradation of 1-chloro-4-nitrobenzene by a newly isolated bacterium, strain LW1
    E Katsivela
    Division of Microbiology, GBF National Research Centre for Biotechnology, D 38124 Braunschweig, Germany
    Appl Environ Microbiol 65:1405-12. 1999
    ..In contrast, when NAD was added in the presence of oxygen, only minor amounts of 5-chloropicolinic acid were formed, and a new product, which exhibited an absorption maximum at 306 nm, accumulated...
  47. ncbi N-removal in a granular sludge sequencing batch airlift reactor
    J J Beun
    Kluyverlaboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Biotechnol Bioeng 75:82-92. 2001
    ..In particular, PHB is stored in bacteria situated in deeper layers of the granules below where the autotrophic activity occurs, serves as a C-source for denitrification...
  48. ncbi Oxygen requirement and tolerance of Campylobacter jejuni
    Nadeem O Kaakoush
    School of Medical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia
    Res Microbiol 158:644-50. 2007
    ..These differences could modulate the outcome of experiments, and may explain discrepancies in the results between strains...
  49. pmc Abundance and genetic diversity of aerobic anoxygenic phototrophic bacteria of coastal regions of the pacific ocean
    Anna E Ritchie
    Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona, USA
    Appl Environ Microbiol 78:2858-66. 2012
    ..Together, these results demonstrate that environmental variables regulate both the abundance and diversity of AAP bacteria but that endemism may also be a contributing factor in structuring these communities...
  50. pmc Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations
    Matthew D Rolfe
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 286:10147-54. 2011
    ..respond to oxygen itself or to metabolic and environmental signals that are sensitive to oxygen availability (aerobiosis)...
  51. pmc Biofilm formation by Campylobacter jejuni is increased under aerobic conditions
    Mark Reuter
    Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, United Kingdom
    Appl Environ Microbiol 76:2122-8. 2010
    ..The increased level of biofilm formation under aerobic conditions is likely to be an adaptation contributing to the zoonotic lifestyle of C. jejuni...
  52. ncbi Expression of the Escherichia coli yfiD gene responds to intracellular pH and reduces the accumulation of acidic metabolic end products
    Neil R Wyborn
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK
    Microbiology 148:1015-26. 2002
    ....
  53. pmc Transcriptome dynamics during the transition from anaerobic photosynthesis to aerobic respiration in Rhodobacter sphaeroides 2.4.1
    Hiroyuki Arai
    Department of Microbiology and Molecular Genetics, University of Texas, Health Science Center at Houston, Houston, TX 77030, USA
    J Bacteriol 190:286-99. 2008
    ..Analyses of these data make it clear that numerous regulatory activities come into play during the transition from one homeostatic state to another...
  54. pmc An iron-regulated alkyl hydroperoxide reductase (AhpC) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen Campylobacter jejuni
    M L Baillon
    School of Biological Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Bacteriol 181:4798-804. 1999
    ..The C. jejuni AhpC protein is an important determinant of the ability of this microaerophilic pathogen to survive oxidative and aerobic stress...
  55. ncbi Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions
    Sagit Shalel-Levanon
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005 1892, USA
    Biotechnol Bioeng 92:147-59. 2005
    ..The results indicate that lower conversion of pyruvate to acetyl-CoA is the main reason for high fluxes through lactate dehydrogenase (LDH) in cultures of the arcA, fnr double mutant strain...
  56. ncbi Physiological responses of Pseudomonas aeruginosa PAO1 to oxidative stress in controlled microaerobic and aerobic cultures
    Wael Sabra
    GBF Gesellschaft für Biotechnologische Forschung mbH, Microbial Systems, Mascheroder Weg 1, 38124 Braunschweig, Germany
    Microbiology 148:3195-202. 2002
    ..It is postulated that the blockage of oxygen transfer may play an important role in the defence of this pathogen against reactive oxygen intermediates...
  57. ncbi The iron-induced ferredoxin FdxA of Campylobacter jejuni is involved in aerotolerance
    A H van Vliet
    Department of Genetics, University of Leicester, UK
    FEMS Microbiol Lett 196:189-93. 2001
    ..jejuni, but significantly reduced aerotolerance of C. jejuni. The fdxA gene is the first reported iron-induced gene of C. jejuni, and encodes a novel component of its oxidative stress defense...
  58. pmc An assessment of the impacts of molecular oxygen on the evolution of proteomes
    Sara Vieira-Silva
    Atelier de BioInformatique, Universite Pierre et Marie Curie Paris 6, Paris, France
    Mol Biol Evol 25:1931-42. 2008
    ..First, we find that aerobiosis is a trait with important phylogenetic inertia but that oxygen content in proteins is not...
  59. ncbi Integrating high-throughput and computational data elucidates bacterial networks
    Markus W Covert
    Bioengineering Department, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0412, USA
    Nature 429:92-6. 2004
    ..We find that a systems biology approach that combines genome-scale experimentation and computation can systematically generate hypotheses on the basis of disparate data sources...
  60. pmc How Escherichia coli is equipped to oxidize hydrogen under different redox conditions
    Michael J Lukey
    Department of Inorganic Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QR, United Kingdom
    J Biol Chem 285:3928-38. 2010
    ..The results have direct relevance for physiological roles of Hyd-1 and Hyd-2, which are expressed in different phases of growth. The properties that we report suggest distinct technological applications of these contrasting enzymes...
  61. pmc Molecular and culture-based analyses of aerobic carbon monoxide oxidizer diversity
    Gary M King
    Darling Marine Center, University of Maine, Walpole, Maine 04573, USA
    Appl Environ Microbiol 69:7257-65. 2003
    ....
  62. pmc Glutathione and catalase provide overlapping defenses for protection against respiration-generated hydrogen peroxide in Haemophilus influenzae
    Bjorn Vergauwen
    Laboratory for Protein Biochemistry and Protein Engineering, Ghent University, 9000 Ghent, Belgium
    J Bacteriol 185:5555-62. 2003
    ....
  63. ncbi Oxygen depletion induced dormancy in Mycobacterium smegmatis
    T Dick
    Institute of Molecular and Cell Biology, Singapore
    FEMS Microbiol Lett 163:159-64. 1998
    ..These results suggest that M. smegmatis might be useful as a fast growing non-pathogenic model for comparative molecular analyses of mycobacterial dormancy...
  64. ncbi Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post-transcriptional regulation of key cellular processes
    Marco J L de Groot
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Microbiology 153:3864-78. 2007
    ....
  65. ncbi Optimization-based framework for inferring and testing hypothesized metabolic objective functions
    Anthony P Burgard
    Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biotechnol Bioeng 82:670-7. 2003
    ....
  66. ncbi State of the art of biogranulation technology for wastewater treatment
    Yu Liu
    Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
    Biotechnol Adv 22:533-63. 2004
    ..This review hopes to provide a platform for developing novel granules-based bioreactors and devising a unified interpretation of the formation of anaerobic and aerobic granules under various operation conditions...
  67. ncbi Fermentative lifestyle in yeasts belonging to the Saccharomyces complex
    Annamaria Merico
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milan, Italy
    FEBS J 274:976-89. 2007
    ..However, these traits have been combined and developed to perfection only in the lineage that underwent the whole-genome duplication and led to the modern Saccharomyces cerevisiae yeast...
  68. pmc Superoxide-mediated amplification of the oxygen-induced switch from [4Fe-4S] to [2Fe-2S] clusters in the transcriptional regulator FNR
    Jason C Crack
    Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Proc Natl Acad Sci U S A 104:2092-7. 2007
    ..This mechanism, a one electron activation of the cluster, coupled to catalytic recycling of the resulting superoxide ion back to oxygen, provides a means of amplifying the sensitivity of [4Fe-4S] FNR to its signal molecule...
  69. pmc The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion
    Adrian J Jervis
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 106:4659-64. 2009
    ..These data lead to a simple model of the FNR switch with physiological implications for the ability of FNR proteins to operate over different ranges of in vivo O(2) concentrations...
  70. ncbi Isolation and characterization of phenol-catabolizing bacteria from a coking plant
    Wael S El-Sayed
    Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
    Biosci Biotechnol Biochem 67:2026-9. 2003
    ..321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase...
  71. pmc The evolution of aerobic fermentation in Schizosaccharomyces pombe was associated with regulatory reprogramming but not nucleosome reorganization
    Zhenguo Lin
    Department of Ecology and Evolution, University of Chicago, USA
    Mol Biol Evol 28:1407-13. 2011
    ..However, we found no significant nucleosome organization change in the promoter of respiration-related gene in Sch. pombe...
  72. pmc ArcB1, a homolog of Escherichia coli ArcB, regulates dimethyl sulfoxide reduction in Shewanella oneidensis MR-1
    Namita P Shroff
    University of Wisconsin Milwaukee, Department of Biological Sciences, 3209 N Maryland Ave, Milwaukee, WI 53211, USA
    J Bacteriol 192:3227-30. 2010
    ..In combination with HptA, ArcB1(So) complemented an E. coli arcB(Ec) mutant. ArcA(So), ArcB1(So), and HptA appear to constitute a two-component signal transduction system that regulates DMSO reduction in S. oneidensis...
  73. ncbi A membrane biofilm reactor achieves aerobic methane oxidation coupled to denitrification (AME-D) with high efficiency
    O Modin
    Department of Urban Engineering, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8656, Japan
    Water Sci Technol 58:83-7. 2008
    ..This study showed that for practical application of the AME-D process, focus should be placed on development of a biofilm reactor...
  74. ncbi Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica
    Tigran V Yuzbashev
    Russian State Collection of Industrial Microorganisms, State Research Institute of Genetics and Selection of Industrial Microorganisms, 1 st Dorozhny pr, 1, Moscow, Russia
    Biotechnol Bioeng 107:673-82. 2010
    ..The possible effect of the mutations on the utilization of different substrates and perspectives of constructing an industrial producer is discussed...
  75. pmc Oxygen is required for the L-cysteine-mediated decomposition of protein-bound dinitrosyl-iron complexes
    Juanjuan Yang
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
    Free Radic Biol Med 49:268-74. 2010
    ....
  76. ncbi Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
    Pavel Bouchal
    Department of Biochemistry, Faculty of Science, Masaryk University, 61137 Brno, Czech Republic
    Biochim Biophys Acta 1804:1350-8. 2010
    ..3) Proteins whose expression was affected mainly by the growth condition. This group contains SSU ribosomal protein S305 / sigma(54) modulation protein, and two short-chain reductase-dehydrogenase proteins...
  77. ncbi Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene
    C L Wang
    Department of Chemical Engineering, University of California, Berkeley 94720-1462, USA
    Appl Microbiol Biotechnol 56:425-30. 2001
    ..Without supplemental cysteine, both the E. coli producing cysteine desulfhydrase and the control E. coli demonstrated minimal cadmium removal...
  78. ncbi Aerobic glucose metabolism of Saccharomyces kluyveri: growth, metabolite production, and quantification of metabolic fluxes
    Kasper Møller
    Center for Process Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, 2800 Lyngby, Denmark
    Biotechnol Bioeng 77:186-93. 2002
    ..cerevisiae at the same growth conditions, where the tricarboxylic acid cycle operates as two branches. This indicates that the respiratory system was not significantly repressed in S. kluyveri during batch cultivation on glucose...
  79. pmc Oxygen, iron, carbon, and superoxide control of the fumarase fumA and fumC genes of Escherichia coli: role of the arcA, fnr, and soxR gene products
    S J Park
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024, USA
    J Bacteriol 177:6255-62. 1995
    ....
  80. ncbi Effect of oxygen limitation on the in vitro antimicrobial susceptibility of clinical isolates of Pseudomonas aeruginosa grown planktonically and as biofilms
    T R Field
    Clinical and Practice Research Group, School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, UK
    Eur J Clin Microbiol Infect Dis 24:677-87. 2005
    ..These results show that oxygen limitation may reduce, in a strain-dependent manner, the susceptibility to tobramycin of P. aeruginosa grown planktonically and as biofilms...
  81. pmc Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol
    Cong T Trinh
    Department of Chemical Engineering and Materials Science and BioTechnology Institute, University of Minnesota, 240 Gortner Laboratory, 1479 Gortner Ave, St Paul, MN 55108, USA
    Appl Environ Microbiol 75:6696-705. 2009
    ..The performance of the designed strain is predicted by the property space of remaining elementary modes...
  82. ncbi Characteristics of the bioreactor landfill system using an anaerobic-aerobic process for nitrogen removal
    Ruo He
    Ministry of Education Key Lab of Environment Remediation and Ecological Health, Zhejiang University, Hangzhou 310029, China
    Bioresour Technol 98:2526-32. 2007
    ..The COD concentration was about 200 mg/l with less than 8 mg/l BOD in recycled leachate at the late stage. In addition, it was found that nitrate in recycled leachate had a negative effect on waste decomposition...
  83. ncbi Ammonium adsorption in aerobic granular sludge, activated sludge and anammox granules
    J P Bassin
    Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
    Water Res 45:5257-65. 2011
    ..The results indicate that adsorption or better ion exchange of ammonium should be incorporated into models for nitrification/denitrification, certainly when aerobic granular sludge is used...
  84. ncbi Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions
    Chandresh Thakker
    Department of Biochemistry and Cell Biology, Rice University, Houston, TX, USA
    Bioresour Technol 130:398-405. 2013
    ..This study demonstrated the use of soluble carbohydrates of the renewable feedstock, soybean as an inexpensive carbon source to produce succinate by fermentation...
  85. ncbi Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO-GAO competition at high temperatures
    M K H Winkler
    Department of Biotechnology, Technische Universiteit Delft, Julianalaan 67, 2628 BC Delft, The Netherlands
    Water Res 45:3291-9. 2011
    ..This study shows that biomass segregation in aerobic granular sludge systems offers an extra possibility to influence microbial competition in order to obtain a desired population...
  86. ncbi Zhongshania antarctica gen. nov., sp. nov. and Zhongshania guokunii sp. nov., gammaproteobacteria respectively isolated from coastal attached (fast) ice and surface seawater of the Antarctic
    Hui Juan Li
    The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China
    Int J Syst Evol Microbiol 61:2052-7. 2011
    ..nov. are proposed. The type strain of Zhongshania antarctica is ZS5-23(T) ( = KACC 14066(T)  = CCTCC AB 209246(T)) and that of Zhongshania guokunii is ZS6-22(T) ( = KACC 14532(T)  = CCTCC AB 209247(T))...
  87. pmc Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae
    G N Vemuri
    Center for Microbial Biotechnology, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Proc Natl Acad Sci U S A 104:2402-7. 2007
    ..Moreover, NADH oxidase restored the deficiency of cytosolic NADH dehydrogenases in S. cerevisiae. These results indicate that NADH oxidase localizes in the cytosol, whereas alternative oxidase is directed to the mitochondria...
  88. ncbi The biological treatment of landfill leachate using a simultaneous aerobic and anaerobic (SAA) bio-reactor system
    Zhiquan Yang
    School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006, PR China
    Chemosphere 72:1751-6. 2008
    ....
  89. ncbi Contribution of aerobic photoheterotrophic bacteria to the carbon cycle in the ocean
    Z S Kolber
    Environmental Biophysics and Molecular Ecology Program, Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ 08901 8521, USA
    Science 292:2492-5. 2001
    ..They are globally distributed in the euphotic zone and represent a hitherto unrecognized component of the marine microbial community that appears to be critical to the cycling of both organic and inorganic carbon in the ocean...
  90. ncbi Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor
    Michael Nielsen
    Department of Microbiology, University of Aarhus, Ny Munkegade, Bldg 540, 8000 Aarhus C, Denmark
    FEMS Microbiol Ecol 51:247-56. 2005
    ..Analysis with O2 and NO2- microsensors showed that the thickness of the activity zones varied as a function of bulk O2 and NO2- concentrations and flow rate...
  91. ncbi Aerobic activation of transcription of the anaerobically inducible Escherichia coli focA-pfl operon by fumarate nitrate regulator
    Francisca Reyes-Ramirez
    Department of Molecular Microbiology, John Innes Centre, Norwich, UK
    FEMS Microbiol Lett 255:262-7. 2006
    ....
  92. ncbi Selective release and function of one of the two FMN groups in the cytoplasmic NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha
    Eddy van der Linden
    Swammerdam Institute for Life Sciences, Biochemistry, University of Amsterdam, The Netherlands
    Eur J Biochem 271:801-8. 2004
    ..The possible function of FMN-a as a hydride acceptor in the hydrogenase reaction catalyzed by the HoxHY dimer is discussed...
  93. ncbi A novel promoter architecture for microaerobic activation by the anaerobic transcription factor FNR
    F A Marshall
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Mol Microbiol 39:747-53. 2001
    ..More or less oxygen reduced expression dramatically. This pattern of response was dependent on the combination of a high-affinity site at the activating class II position and a lower affinity site at the upstream position...
  94. ncbi Respiratory pathways of Rhodobacter sphaeroides 2.4.1(T): identification and characterization of genes encoding quinol oxidases
    N J Mouncey
    Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Medical School, Houston, TX 77030, USA
    FEMS Microbiol Lett 192:205-10. 2000
    ..No role was found for the Qox oxidase, nor was a qoxB::lacZ transcriptional fusion expressed under a variety of conditions. These are the first molecular studies to characterize the quinol oxidases of R. sphaeroides 2.4.1(T)...
  95. ncbi The effects of shear force on the formation, structure and metabolism of aerobic granules
    J H Tay
    Environmental Engineering Research Center, School of Civil and Structural Engineering, Nanyang Technological University, Singapore
    Appl Microbiol Biotechnol 57:227-33. 2001
    ..This research provides experimental evidence to show that shear force plays a crucial role in aerobic granulation and further influences the structure and metabolism of granules...
  96. ncbi Characterization of aerobic and anaerobic vegetative growth of the food-borne pathogen Bacillus cereus F4430/73 strain
    Eric Rosenfeld
    Laboraatoire de Biotechnologies et Chimie Bio organique FRE 2766 CNRS, Batiment Marie Curie, Pôle Sciences et Technologies, Universite de La Rochelle, 17042 La Rochelle Cedex 1, France
    Can J Microbiol 51:149-58. 2005
    ..Growth and metabolic features of B. cereus F4430/73 are discussed using biochemical and genomic data...
  97. ncbi Size-effect on the physical characteristics of the aerobic granule in a SBR
    S K Toh
    Environmental Engineering Research Centre, School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
    Appl Microbiol Biotechnol 60:687-95. 2003
    ..67. In view of biological viability and physical properties, the operational size-range suggested for optimal performance and economically effective aerobic SBR granular sludge is a diameter of 1.0-3.0 mm...
  98. ncbi Relationship between size and mass transfer resistance in aerobic granules
    Y Q Liu
    Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
    Lett Appl Microbiol 40:312-5. 2005
    ..To investigate the size effect of aerobic granules on mass transfer efficiency by introducing the effective factor and the modified Thiele modulus...
  99. ncbi Possible role of denitrification on aerobic granular sludge formation in sequencing batch reactor
    Junfeng Wan
    Universite de Toulouse, INSA, UPS, INP, LISBP, France
    Chemosphere 75:220-7. 2009
    ..The presence of nitrate (commonly provided by nitrification) should be considered as a possible factor which helps to maintain the granulation process...
  100. ncbi The effect of oxygen on biochemical networks and the evolution of complex life
    Jason Raymond
    Microbial Systems Division, Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA
    Science 311:1764-7. 2006
    ....
  101. pmc ErpA, an iron sulfur (Fe S) protein of the A-type essential for respiratory metabolism in Escherichia coli
    Laurent Loiseau
    Unité Propre de Recherche 9043, Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Proc Natl Acad Sci U S A 104:13626-31. 2007
    ..coli. Surprisingly, the erpA gene maps at a distance from any other Fe-S biogenesis-related gene. ErpA is an A-type Fe-S protein that is characterized by an essential role in cellular metabolism...

Research Grants46

  1. Genetic Control of Tubulin Function in Development
    ELIZABETH RAFF; Fiscal Year: 2005
    ..The primary aim is to determine how transient expression of beta3-tubulin during development modulates the microtubule cytoskeleton in the differentiated cell. ..
  2. Iron Trafficking to Ribonucleotide Reductases
    DANIEL KOSMAN; Fiscal Year: 2006
    ..This work should provide new insight into the assembly of a critical iron prosthetic group in vivo and evidence that this process can be a target for intervention in a viral infection. ..
  3. Managing Ionic Iron: Molecular Architecture and Mechanism of Cell Iron Metabolism
    Daniel J Kosman; Fiscal Year: 2010
    ..An understanding of how cells suppress this chemistry would make a significant contribution to our eventual elucidation of the molecular basis for the multitude of human pathologies often attributed in part to mismanaged ionic iron. ..
  4. B.pseudomallei bioterrorism and quorum sensing
    DANIEL HASSETT; Fiscal Year: 2003
    ..These studies will provide important information that will lead to countermeasures against a potential bioterrorist attack using the potentially lethal gram-negative bacterium, B. pseudomallei. ..
  5. Proteogenome of Anaerobic P. aeruginosa in CF Mucus
    DANIEL HASSETT; Fiscal Year: 2003
    ..Our LONG TERM GOAL is to identify cellular targets that inhibit or significantly compromise the denitrification process that is occurring by P. aeruginosa in the CF airways. ..
  6. COPPER METALLOBIOCHEMISTRY IN SACCHAROMYCES CEREVISAE
    DANIEL KOSMAN; Fiscal Year: 2002
    ..Thus, elucidation of the function and modulation of Mac1p in yeast will be broadly relevant to eukaryotic copper and iron metabolism in general. ..
  7. IRON, FUR AND ALGINATE PRODUCTION IN P AERUGINOSA
    DANIEL HASSETT; Fiscal Year: 2002
    ..Information derived from the studies proposed herein will advance the current knowledge about the role of these factors in the pathogenesis of P. aeruginosa infections in CF. ..
  8. Role of OxyR in P. aeruginosa Biofilm Resistance to H202
    DANIEL HASSETT; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  9. Production of Recombinant Eukaryotic Ferroxidases as Protein Therapeutics
    DANIEL KOSMAN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  10. Regulation of hemolysin genes in Bacillus
    Michiko Nakano; Fiscal Year: 2007
    ..subtilis. [unreadable] [unreadable] [unreadable]..
  11. ANAEROBIC EXPRESSION OF FUMARATE REDUCTASE IN E. COLI
    Robert Gunsalus; Fiscal Year: 2008
    ..The Nar and Mode gene families will be identified by microarray analysis. Finally, the physiology of nitrate control will be examined by use of continuous culture methods. [unreadable] [unreadable]..
  12. ROS-induced Nucleic Acid Damage
    Yinsheng Wang; Fiscal Year: 2006
    ..The outcome of the research proposed here will provide better understanding of the biological consequences of oxidative damage on nucleic acids by ROS and their implications in human health. ..
  13. Pd-Catalyzed Olefin Functionalization Reactions for Organic Synthesis
    MATTHEW SIGMAN; Fiscal Year: 2007
    ..The proposed research described herein will be approached using method discovery and development facilitated by elucidation of mechanistic details using physical organic chemistry techniques. ..
  14. Detection of Biofilm Bacteria using GloBac
    Robin Patel; Fiscal Year: 2008
    ..The goal of the proposed studies is to develop a rapid test for detection of bacteria on artificial joints that can be used in the operating room to diagnose infected artificial joints. [unreadable] [unreadable] [unreadable]..
  15. FASEB Summer Conference: Folic Acid, Vitamin B12 and One Carbon Metabolism
    Ruma Banerjee; Fiscal Year: 2008
    ..The poster sessions highlight the activities of the younger participants and the number of short oral presentations chosen from abstracts submitted by junior attendees has been increased. [unreadable] [unreadable] [unreadable]..
  16. Promoting Healthy Weight with "Stability First"
    Michaela Kiernan; Fiscal Year: 2010
    ..g., depressive symptoms), and weight stability;(2b) examine baseline moderators (e.g., gender);and (2c) investigate mediators (e.g., deprivation, effort and attention, and self-efficacy). ..
  17. Photoreceptor Cell Vulnerability and Glutathione Status
    Barry S Winkler; Fiscal Year: 2010
    ..These outcomes should help in the design of therapeutic treatments for photoreceptor degenerations that are linked to an inadequate antioxidative defense mechanism. ..
  18. Post-translational Modifications of High-mobility Group Proteins
    Yinsheng Wang; Fiscal Year: 2009
    ..In addition, the outcome of proposed studies may provide important molecular biomarkers for cancer diagnosis and prognosis. ..
  19. Vascular and Neuro-Inflammatory Effects of Endurance Exercise Training in African
    Bo Fernhall; Fiscal Year: 2010
    ..This project evaluates the effect of endurance exercise training as a preventive behavioral intervention to lower the risk of these diseases in young African-Americans. ..
  20. Pilot-scale Research of Novel Amendment Delivery for in-situ Sediment Remediation
    Upal Ghosh; Fiscal Year: 2009
    ..This field research will evaluate the effectiveness of a novel approach to alter the binding capacity of sediments to reduce human exposure to such contaminants. ..
  21. REGULATION OF E COLI CYTOCHROME OXIDASE AND TCA CYCLE
    Robert Gunsalus; Fiscal Year: 2001
    ..coli. These studies should provide a detailed understanding of how the cell coordinates pathways for electron and carbon flow within the cell during transition between oxygen rich and oxygen limiting conditions. ..
  22. High-force eccentric exercise for sarcopenia
    Stan Lindstedt; Fiscal Year: 2002
    ....
  23. METABOLIC AND HOMEOSTATIC MECHANISMS IN RETINA AND RPE
    Barry Winkler; Fiscal Year: 2003
    ..g., diamox) which have been reported to decrease retinal edema. A long-term goal of the second project is to understand better the nature of the interactions between the retina and RPE and factors regulating retinal detachment. ..
  24. Blood vessels from adult versus embryonic stem cells
    David Manka; Fiscal Year: 2004
    ..The objective is to provide novel insights into the potential benefits and limitations of each type of stem cell that may be relevant in treating human vascular diseases. ..
  25. NOVEL THERAPIES FOR ACETAMINOPHEN TOXICITY
    Laura James; Fiscal Year: 2004
    ..abstract_text> ..
  26. Characterization of the Coxiella Dot/lcm homologues
    JOSEPH VOGEL; Fiscal Year: 2006
    ..pneumophila Dot/Icm proteins. We propose to determine if the C. burnetii Dot/Icm homologues are needed for this pathogen to replicate inside host cells and to identify proteins secreted by this system. ..
  27. HUMAN RETROVIRUS-5 AND ARTHRITIS
    Robin Patel; Fiscal Year: 2002
    ..Identification of a specific infectious agent associated with these arthritides would potentially allo for the development of preventive strategies such as vaccination and novel therapeutic approaches. ..
  28. MICROBIAL METABOLISM OF REDUCED PHOSPHORUS COMPOUNDS
    WILLIAM METCALF; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  29. Enhancement of antimicrobics by electrical current
    Robin Patel; Fiscal Year: 2007
    ..This novel but simple resistance reversing strategy may generalize to a wide variety of antimicrobics and microorganisms and a wide variety of biofilm-associated human infections. [unreadable] [unreadable] [unreadable] [unreadable]..
  30. The Sulfur/Glutamate Circuitry in the Neuroimmune System
    Ruma Banerjee; Fiscal Year: 2009
    ..We propose to study the metabolic interdependence between astrocytes, neurons and T cells in performing these critical functions under normal and neurotoxic conditions. ..
  31. The molecular mechanism of Legionella type IV secretion
    Joseph P Vogel; Fiscal Year: 2010
    ..pneumophila secretion apparatus is likely to be applicable to understanding the molecular mechanisms of virulence used by other pathogens, and could serve as the basis to prevent or treat a number of different diseases. ..
  32. OXYGEN SENSING BY THE RESPIRATORY CHAIN
    KURT KWAST; Fiscal Year: 2005
    ....
  33. RNAi Mediated Gene Disruption in Caenorhabditis Elegans
    Wayne Van Voorhies; Fiscal Year: 2006
    ..Such worms could identify candidate genes affecting processes that act "downstream" from the metabolic rate and provide valuable insights into the causes of aging and mechanisms by which it can be attenuated. ..
  34. 2008 Thiol-based Redox Regulation and Signaling Gordon Research Conference
    Ruma Banerjee; Fiscal Year: 2008
    ..g. in biophysical methods, bioinformatics and animal model systems, with physicians, the meeting is expected to further stimulate collaborations and catalyze scientific progress. (End of Abstract) [unreadable] [unreadable] [unreadable]..
  35. Evaluation of a Biomarker for Acetaminophen Toxicity
    Laura James; Fiscal Year: 2005
    ..The data generated from this study will lead to enhanced recognition of APAP hepatotoxicity, thus allowing for better identification of "at risk" populations and the creation of prevention strategies. ..
  36. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  37. Physiological Signals to MCT Expression
    George Brooks; Fiscal Year: 2008
    ..Aim 3 studies will involve comparisons of the levels of muscle MCT pre-mRNAs, mRNAs and protein levels. We have the tools to achieve the stated aims. ..
  38. Anaerobic Regulatory Pathways in a Periodontopathogen
    David Kolodrubetz; Fiscal Year: 2006
    ..Characterizing undefined, potentially new transcriptional regulatory pathways is important because the proteins involved may serve as targets for future drug development. ..
  39. Role of iNOS and CAT-2 in defense against CP infection
    Jody Gookin; Fiscal Year: 2006
    ..parvum infection. The PI is presently in the 3rd year of a 5-year K08 from NIDDK. These studies are anticipated to generate key preliminary data for the PI's first R01 application ..
  40. SECRETION MECHANISM OF A NOVEL C. RECTUS S-LAYER PROTEIN
    David Kolodrubetz; Fiscal Year: 2002
    ..Finally, the transport mutants will be examined for the possible mis-localization of non-S-layer proteins since there are two reports of type I transport pathways transporting more than one virulence protein. ..
  41. NO& Epithelial Repair in Cryptosporidiosis
    Jody Gookin; Fiscal Year: 2006
    ..abstract_text> ..
  42. Exercise Substrate Utilization:The Crossover Concept
    George Brooks; Fiscal Year: 2006
    ..Further, we have the auxiliary personnel and facilities to conduct longitudinal training studies ..
  43. FUNCTION AND ASSEMBLY OF CO2 ASSIMILATORY ENZYMES
    F Tabita; Fiscal Year: 2005
    ..Of particular interest will be the relationship of protein folding and catalysis to cellular function. ..
  44. MOLECULAR BASIS AND ENZYMOLOGY OF MICROBIAL BIOSYNTHESIS
    F Tabita; Fiscal Year: 2003
    ..For each enzyme, recombinant systems will be used to facilitate efforts to learn how these proteins function specifically in biosynthesis and how control of their activity influences normal cellular metabolism. ..
  45. Single Muscle Fiber Contractile Properties With Aging
    Scott Trappe; Fiscal Year: 2005
    ..With this information we will be able to determine, for the first time, the interaction of aging, resistance training, and gender on single muscle fiber contractile function. ..
  46. Environmental Regulation of Staphylococcus epidermidis PIA Synthesis
    GREG SOMERVILLE; Fiscal Year: 2007
    ..The work contained in this proposal is a first step toward inhibiting the formation of this complex sugar, preventing the formation of a bacterial biofilm, and providing physicians a way to treat patients. [unreadable] [unreadable]..