seminiferous tubules

Summary

Summary: The convoluted tubules in the TESTIS where sperm are produced (SPERMATOGENESIS) and conveyed to the RETE TESTIS. Spermatogenic tubules are composed of developing germ cells and the supporting SERTOLI CELLS.

Top Publications

  1. ncbi PKCtheta II, a new isoform of protein kinase C specifically expressed in the seminiferous tubules of mouse testis
    Y S Niino
    Institute of Molecular Oncology, Department of Pathology, Faculty of Dentistry and Center for Biotechnology, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Biol Chem 276:36711-7. 2001
  2. pmc Sertoli cell behaviors in developing testis cords and postnatal seminiferous tubules of the mouse
    Liesl Nel-Themaat
    Department of Genetics, University of Texas M D Anderson Cancer Center, Houston, Texas, 77005, USA
    Biol Reprod 84:342-50. 2011
  3. ncbi Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules
    S Yang
    Department of Urology, Sperm Development and Genetics Laboratory, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Cell Prolif 45:91-100. 2012
  4. ncbi Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis
    Noora Kotaja
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM, Universite Louis Pasteur, B P 163, 67404 Illkirch, Strasbourg, France
    Nat Methods 1:249-54. 2004
  5. ncbi Mouse germ line stem cells undergo rapid and stochastic turnover
    Allon M Klein
    Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Stem Cell 7:214-24. 2010
  6. ncbi Ultrastructural study on cytotoxic effects of cyclosporine A in spermiogenesis in rats
    Hiroshi Masuda
    Department of Urology, Osaka Medical College, Takatsuki, Japan
    Med Electron Microsc 36:183-91. 2003
  7. ncbi De novo morphogenesis of seminiferous tubules from dissociated immature rat testicular cells in xenografts
    Kathrin Gassei
    University of Pittsburgh School of Medicine, Center for Research in Reproductive Physiology, Department of Cell Biology and Physiology, 3500 Terrace St, S362 Biomedical Science Towers, Pittsburgh PA 15261, USA
    J Androl 27:611-8. 2006
  8. ncbi Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules
    Kaoru Kita
    Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Biol Reprod 76:211-7. 2007
  9. ncbi Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:985-91. 2005
  10. ncbi Estradiol acts as a germ cell survival factor in the human testis in vitro
    V Pentikäinen
    Hospital for Children and Adolescents, University of Helsinki, Finland
    J Clin Endocrinol Metab 85:2057-67. 2000

Research Grants

  1. Male germline stem cell culture and genetic modification
    RALPH BRINSTER; Fiscal Year: 2007
  2. Drug Transport at the Blood-Testis Barrier
    Nathan J Cherrington; Fiscal Year: 2013
  3. BDADs for Male Contraception
    John K Amory; Fiscal Year: 2013
  4. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
  5. Epigenetic Reprogramming in Germline by Phthalates
    Kwan Hee Kim; Fiscal Year: 2013
  6. Nongenomic Androgen Signaling in Sertolli Cells
    William H Walker; Fiscal Year: 2010
  7. Genetic analysis of male gonadal development
    Mary C Mullins; Fiscal Year: 2013
  8. SERTOLI CELL-GERM CELL SURFACE MEMBRANE INTERACTIONS
    ROBERT DEPHILIP; Fiscal Year: 1991
  9. MECHANISMS OF TOXICITY IN TESTES
    Michael Welsh; Fiscal Year: 1999
  10. Regulation of Gene Expression During Spermatogenesis
    Peter Petrusz; Fiscal Year: 2004

Detail Information

Publications272 found, 100 shown here

  1. ncbi PKCtheta II, a new isoform of protein kinase C specifically expressed in the seminiferous tubules of mouse testis
    Y S Niino
    Institute of Molecular Oncology, Department of Pathology, Faculty of Dentistry and Center for Biotechnology, Showa University, 1 5 8 Hatanodai, Shinagawa ku, Tokyo 142 8555, Japan
    J Biol Chem 276:36711-7. 2001
    ..chain reaction of microdissected tissues clearly demonstrated that PKCthetaII is expressed in the seminiferous tubules of the mouse testis...
  2. pmc Sertoli cell behaviors in developing testis cords and postnatal seminiferous tubules of the mouse
    Liesl Nel-Themaat
    Department of Genetics, University of Texas M D Anderson Cancer Center, Houston, Texas, 77005, USA
    Biol Reprod 84:342-50. 2011
    Sertoli cells are the primary structural component of the fetal testis cords and postnatal seminiferous tubules. Live imaging technologies facilitate the visualization of cell morphologies and behaviors through developmental processes...
  3. ncbi Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules
    S Yang
    Department of Urology, Sperm Development and Genetics Laboratory, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    Cell Prolif 45:91-100. 2012
    ..However, up to now it is not known whether iPS cells would be able to differentiate into male germ cells in vivo. The aim of this study was to explore differentiation potential of iPS cells to male germ cells in vitro and in vivo...
  4. ncbi Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis
    Noora Kotaja
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM, Universite Louis Pasteur, B P 163, 67404 Illkirch, Strasbourg, France
    Nat Methods 1:249-54. 2004
  5. ncbi Mouse germ line stem cells undergo rapid and stochastic turnover
    Allon M Klein
    Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Stem Cell 7:214-24. 2010
    ..These results suggest that short-lived stem cells may be a common feature of mammalian stem cell systems and reveal a natural mechanism for matching the rates of cell proliferation and loss in tissue...
  6. ncbi Ultrastructural study on cytotoxic effects of cyclosporine A in spermiogenesis in rats
    Hiroshi Masuda
    Department of Urology, Osaka Medical College, Takatsuki, Japan
    Med Electron Microsc 36:183-91. 2003
    ..A prominent change in the seminiferous tubules was an occurrence of numerous residual bodies containing one or more flagella...
  7. ncbi De novo morphogenesis of seminiferous tubules from dissociated immature rat testicular cells in xenografts
    Kathrin Gassei
    University of Pittsburgh School of Medicine, Center for Research in Reproductive Physiology, Department of Cell Biology and Physiology, 3500 Terrace St, S362 Biomedical Science Towers, Pittsburgh PA 15261, USA
    J Androl 27:611-8. 2006
    ..The grafts were vascularized and contained elongated seminiferous tubules. Histologic analysis revealed the presence of a basement membrane, a histologically normal interstitium ..
  8. ncbi Production of functional spermatids from mouse germline stem cells in ectopically reconstituted seminiferous tubules
    Kaoru Kita
    Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Biol Reprod 76:211-7. 2007
    Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells...
  9. ncbi Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:985-91. 2005
    ..This culture system will provide a new opportunity for understanding the regulatory mechanism that governs spermatogonial stem cells...
  10. ncbi Estradiol acts as a germ cell survival factor in the human testis in vitro
    V Pentikäinen
    Hospital for Children and Adolescents, University of Helsinki, Finland
    J Clin Endocrinol Metab 85:2057-67. 2000
    ..effectively inhibited male germ cell apoptosis, which was induced in vitro by incubating segments of human seminiferous tubules without survival factors (i.e. serum and hormones)...
  11. pmc Spermatogenesis following male germ-cell transplantation
    R L Brinster
    Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104
    Proc Natl Acad Sci U S A 91:11298-302. 1994
    ..that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules. Donor cell spermatogenesis in recipient testes showed normal morpholigical characteristics and produced ..
  12. ncbi Seminiferous tubule degeneration and infertility in mice with sustained activation of WNT/CTNNB1 signaling in sertoli cells
    Alexandre Boyer
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Biol Reprod 79:475-85. 2008
    ..mice were sterile because of testicular atrophy starting at 5 wk of age, associated with degeneration of seminiferous tubules and the progressive loss of germ cells...
  13. ncbi Extracellular purines from cells of seminiferous tubules
    Daniel Pens Gelain
    Laboratório de Transdução de Sinal em Células Testiculares, Departamento de Bioquimica, Instituto de Ciencias Basicas da Saude, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
    Mol Cell Biochem 245:1-9. 2003
    ..communication mediated by purines were proposed; however, the cellular source(s) of these molecules in seminiferous tubules remains unknown...
  14. ncbi Desert hedgehog (Dhh) gene is required in the mouse testis for formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules
    A M Clark
    Curis, Inc, Cambridge, Massachusetts 02138, USA
    Biol Reprod 63:1825-38. 2000
    ..contributed to other characteristics, such as extracordal gonocytes, apolar Sertoli cells, and anastomotic seminiferous tubules. The two DHH:-null phenotypes described have common peritubular cell defects that may be indicative of the ..
  15. ncbi Non-random distribution of spermatogonia in rats: evidence of niches in the seminiferous tubules
    H Chiarini-Garcia
    Laboratory of Cellular Biology, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, MG 31270 901, Brazil
    Reproduction 126:669-80. 2003
    ..the results of this study strongly indicate that the most primitive type A spermatogonia (A(s), A(paired) and A(al)) in rats are present in niches located in those areas of the seminiferous tubules that border the interstitial tissue.
  16. ncbi Germline stem cell transplantation and transgenesis
    Ralph L Brinster
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3850 Baltimore Avenue, Philadelphia, PA 19104, USA
    Science 296:2174-6. 2002
    ..The technique also is being used to examine spermatogenic defects, correct male infertility, and generate transgenic animals...
  17. pmc Toxicant-induced leakage of germ cell-specific proteins from seminiferous tubules in the rat: relationship to blood-testis barrier integrity and prospects for biomonitoring
    Naomi D Elkin
    Medical Research Council Human Reproductive Sciences Unit, Centre for Reproductive Biology, The Queen s Medical Research Institute, Edinburgh EH16 4TJ, UK
    Toxicol Sci 117:439-48. 2010
    ..Previous studies suggested that disruption of spermatogenesis induced leakage of germ cell proteins from seminiferous tubules (STs) into interstitial fluid (IF); such proteins have potential for use as biomarkers...
  18. ncbi Immunofluorescence distribution of actin-associated proteins in human seminiferous tubules of adolescent testes, normal and pathologic
    G Santoro
    Department of Biomorphology, University of Messina, Italy
    J Endocrinol Invest 23:369-75. 2000
    The aim of our study on human seminiferous tubules of adolescent testes was to study the localization of two actin-associated proteins of the adherens junctions, such as vinculin and talin, and to verify if there were modifications in ..
  19. ncbi The effects of an electromagnetic field on the boundary tissue of the seminiferous tubules of the rat: A light and transmission electron microscope study
    A A Khaki
    Department of Anatomy, Tabriz University of Medical Sciences, Tabriz, Iran
    Folia Morphol (Warsz) 65:188-94. 2006
    ..of EMF in order to observe recovery, if any, from the changes induced by EMF on the boundary tissue of the seminiferous tubules. The materials were processed and observed under a light and a transmission electron microscope...
  20. ncbi CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules
    Shuang Shi
    Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, 38 Xueyuan Road, Beijing 100083, China
    Int J Biochem Cell Biol 37:1633-40. 2005
    ..The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis...
  21. ncbi Perturbation of spermatogenesis by androgen antagonists directly injected into seminiferous tubules of live mice
    Kaz Nagaosa
    Graduate School of Natural Science and Technology and Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920 1192, Japan
    Reproduction 133:21-7. 2007
    ..We injected the androgen antagonists, flutamide and vinclozolin, directly into seminiferous tubules of live mice, which had been treated with busulfan for a temporal arrest of spermatogenesis, and the testes ..
  22. ncbi Stages and duration of spermatogenesis in the domestic ferret (Mustela putorius furo)
    M Nakai
    Department of Animal Sciences, University of Illinois at Urbana Champaign, 1207 West Gregory Drive, Urbana, IL 61801, USA
    Tissue Cell 36:439-46. 2004
    Classification of seminiferous tubules is the basis for understanding normal and abnormal spermatogenesis...
  23. ncbi Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer
    A K Faure
    Unite INSERM U309, Universite Joseph Fourier, Institut Albert Bonniot, Faculte de Medecine de Grenoble, Domaine de la Merci, 38706 La Tronche Cedex, France
    Mol Hum Reprod 9:757-63. 2003
    ..A similar observation was made in the peritumoral seminiferous tubules of testicular tumour tissues, whenever they were lacking germinal cells, with carcinoma in situ (CIS) cells ..
  24. ncbi Connexin gene expression in seminiferous tubules of the Sprague-Dawley rat
    M S Risley
    Department of Biological Sciences, Fordham University, Bronx, NY 10458, USA
    Biol Reprod 62:748-54. 2000
    ..chain reaction (RT-PCR) analyses showed that 11 Cx mRNAs were present in total RNA from seminiferous tubules and that 10 of the Cx mRNAs were present in polysomes and presumably translated...
  25. ncbi Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus)
    Tânia M Segatelli
    Department of Morphofisiological Science, DCM Bloco H 79, University of State of Paraná UEM, Av Colombo, 5790, Maringa, Parana, Brazil
    J Androl 25:872-80. 2004
    ..Based on the volume occupied by seminiferous tubules in the testis and the tubular diameter, about 9 and 18 m of seminiferous tubules were found per testis and ..
  26. ncbi Impaired expression and distribution of adherens and gap junction proteins in the seminiferous tubules of rats undergoing autoimmune orchitis
    C Perez
    Instituto de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
    Int J Androl 34:e566-77. 2011
    ..EAO) is characterized by an interstitial lymphomononuclear cell infiltration and a severe lesion of seminiferous tubules (ST) with germ cells that undergo apoptosis and sloughing...
  27. pmc Excess type I interferon signaling in the mouse seminiferous tubules leads to germ cell loss and sterility
    Anne Pascale Satie
    INSERM, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F 35042 Rennes, France
    J Biol Chem 286:23280-95. 2011
    ..Type I interferon signaling may lead to idiopathic infertilities by affecting the interplay between germ cells and Sertoli cells...
  28. ncbi Effect of formaldehyde inhalation on Hsp70 in seminiferous tubules of rat testes: an immunohistochemical study
    Oguz Aslan Ozen
    Department of Anatomy, Faculty of Medicine, Afyon Kocatepe University, Turkey
    Toxicol Ind Health 21:249-54. 2005
    ..Diameters of seminiferous tubules and serum testosterone levels in animals inhaling FA were significantly decreased...
  29. ncbi Characterisation of the biological effects of neurohypophysial peptides on seminiferous tubules
    G C Harris
    Department of Anatomy, School of Medical Sciences, University of Bristol, UK
    J Endocrinol 156:35-42. 1998
    ..A small section of tubule was placed in a microslide chamber and perifused with medium. Seminiferous tubules were treated with OT (2 nM), [Arg8]-vasopressin (AVP, 0.2 nM) or [Thr4,Gly7]-OT (TGOT, 2 nM, 8 nM and 0...
  30. ncbi 17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules
    Camille Bois
    Universite de Caen Basse Normandie, EA 2608, Laboratoire Estrogènes et Reproduction, Esplanade de la Paix, F 14032 Caen Cedex, France
    J Mol Endocrinol 48:89-97. 2012
    ..In order to enlighten the potential role of estrogen in rat spermatogenesis, seminiferous tubules (ST) from two groups of seminiferous epithelium stages (II-VIII and IX-I) were treated with either 17β-..
  31. ncbi Morphogenesis of the bovine rete testis: the intratesticular rete and its connection to the seminiferous tubules
    K H Wrobel
    Institute of Anatomy, University of Regensburg, Germany
    Anat Embryol (Berl) 202:475-90. 2000
    ..Only the thick outer cords transform into the permanent seminiferous tubules, whereas the thinner cords in the central zone are transitory structures that disappear between 45 and 110 ..
  32. pmc Trypanosoma cruzi and myoid cells from seminiferous tubules: interaction and relation with fibrous components of extracellular matrix in experimental Chagas' disease
    Luiz Otávio Pereira Carvalho
    Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
    Int J Exp Pathol 90:52-7. 2009
    ..histopathological examination showed alterations in the extracellular matrix of the lamina propria of the seminiferous tubules. Furthermore, amastigotes were present in seminiferous tubules, within myoid cells, and in the adjacencies ..
  33. ncbi Three-dimensional analysis of connexin 43 gap junction in the ex vivo rat seminiferous tubules: short-term effects of hormonal effectors
    Jerome Gilleron
    INSERM U 895, Team 5 Physiopathologic control of germ cell proliferation genomic and non genomic mechanisms, Paris, France
    Microsc Res Tech 72:845-55. 2009
    ..few and indirect observations have been reported on the distribution of Cx43, the predominant Cx within the seminiferous tubules. In the present study, we developed an innovative method that allows visualization of the three- ..
  34. ncbi Histological and ultrastructural analysis of the seminiferous tubule wall in ageing testis
    O T Pop
    Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, Romania
    Rom J Morphol Embryol 52:241-8. 2011
    ..Our results showed that BM thickness is apparently decreasing with ageing whereas LP presents extremely variable degenerative changes, with a "mosaic", focal distribution and no tendency to advance with ageing...
  35. ncbi Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules
    A G Abdou
    Pathology Department, Menofiya University, Shebein Elkom, Egypt
    Andrologia 43:57-64. 2011
    ..on cyclin A expression in various human spermatogenic disorders and its relationship to the morphology of seminiferous tubules are not well clarified...
  36. ncbi The microtubule plus end-binding protein EB1 is involved in Sertoli cell plasticity in testicular seminiferous tubules
    Fubin Wang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People s Republic of China
    Exp Cell Res 314:213-26. 2008
    ..Seminiferous tubule circularity and germ cell number were also reduced. These data indicate a requirement of proper microtubule arrays for Sertoli cell plasticity and function in testis...
  37. ncbi Human 'testicular dysgenesis syndrome': a possible model using in-utero exposure of the rat to dibutyl phthalate
    Jane S Fisher
    MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, The Chancellor s Building, University of Edinburgh, 49 Little France Crescent, Edinburgh EH16 4SB, UK
    Hum Reprod 18:1383-94. 2003
    ..The disorders comprising human 'testicular dysgenesis syndrome' (TDS) may be increasing in incidence. TDS originates in fetal life but the mechanisms are not known, and discerning them requires an animal model...
  38. ncbi Diethylstilbestrol induces rat spermatogenic cell apoptosis in vivo through increased expression of spermatogenic cell Fas/FasL system
    Radhika Nair
    National Institute of Immunology, Aruna Asaf Ali Road, New Delhi, India 110067
    J Biol Chem 278:6470-81. 2003
    ....
  39. pmc Dose-dependent effects on cell proliferation, seminiferous tubules, and male germ cells in the fetal rat testis following exposure to di(n-butyl) phthalate
    Kim Boekelheide
    Brown University, Providence, Rhode Island 02912, USA
    Microsc Res Tech 72:629-38. 2009
    ..Cell proliferation and apoptosis are key processes regulating development of the testis, and alterations in these processes may underlie testicular dysgenesis...
  40. ncbi H3.5 is a novel hominid-specific histone H3 variant that is specifically expressed in the seminiferous tubules of human testes
    Raphael Schenk
    HELIOS Children s Hospital, HELIOS Klinikum Wuppertal, Research Laboratory, Witten Herdecke University, Germany
    Chromosoma 120:275-85. 2011
    ..H3.5 mRNA is specifically expressed in seminiferous tubules of human testis. Interestingly, H3...
  41. ncbi Evaluation of candidate markers for the peritubular myoid cell lineage in the developing mouse testis
    Angela Jeanes
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Reproduction 130:509-16. 2005
    ..Although not strictly specific for PM cells, these markers are likely to be useful in studying the biology of early PM cells in the fetal testis...
  42. ncbi Peritubular cell-Sertoli cell interactions: factors involved in PmodS activity
    G Verhoeven
    Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Catholic University of Leuven, Belgium
    Andrologia 32:42-5. 2000
    ..It is concluded that, until PModS has been identified unambiguously, the hypothesis that it acts as an essential andromedin in the testis should be regarded with caution...
  43. ncbi Sertoli cells initiate testicular innate immune responses through TLR activation
    Anna Riccioli
    Department of Histology and Medical Embryology, Istituto Pasteur Fondazione Cenci Bolognetti, University of Rome La Sapienza, Rome, Italy
    J Immunol 177:7122-30. 2006
    ..Collectively, this study demonstrates the novel ability of Sertoli cells to potentially respond to a wide variety of bacteria through TLR stimulation...
  44. ncbi Premeiotic germ cell defect in seminiferous tubules of Atm-null testis
    Keiyo Takubo
    Department of Cell Differentiation, The Sakaguchi Laboratory of Developmental Biology, Keio University School of Medicine, 35 Shinano machi, Shinjuku ku, Tokyo 160 8582, Japan
    Biochem Biophys Res Commun 351:993-8. 2006
    ..It has been shown that ataxia telangiectasia-mutated (ATM) is required for meiotic division of the seminiferous tubules. Here, we show that, in addition to its role in meiosis, ATM has a pivotal role in premeiotic germ cell ..
  45. ncbi Ultrastructural findings in murine seminiferous tubules as a consequence of subchronic vanadium pentoxide inhalation
    Teresa Imelda Fortoul
    Cellular and Tisular Biology Department, School of Medicine, National University of Mexico, CP 04510 Mexico City, Mexico
    Reprod Toxicol 23:588-92. 2007
    ..The relevance of this report concerns the impact that metal air pollution could have on male fertility in dense cities with vehicular traffic problems...
  46. pmc The multi-copy mouse gene Sycp3-like Y-linked (Sly) encodes an abundant spermatid protein that interacts with a histone acetyltransferase and an acrosomal protein
    Louise N Reynard
    Division of Developmental Genetics and Stem Cell Biology, Medical Research Council National Institute for Medical Research, London, United Kingdom
    Biol Reprod 81:250-7. 2009
    ..Together, these data suggest SLY1 may be involved in multiple processes during spermiogenesis, including the control of gene expression and the development or function of the acrosome...
  47. pmc Regulation of Sertoli-germ cell adhesion and sperm release by FSH and nonclassical testosterone signaling
    John Shupe
    Center for Research in Reproductive Physiology, Department of Cell Biology and Molecular Physiology, Magee Women s Research Institute, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Mol Endocrinol 25:238-52. 2011
    ..In contrast, FSH acts to limit testosterone-mediated ERK kinase activity and germ cell attachment...
  48. ncbi Stereological and morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human chorionic gonadotrophin
    Luciano A Favorito
    Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, Brazil
    Int Braz J Urol 31:562-6; discussion 567-8. 2005
    Quantify the distribution of collagen and analyze the seminiferous tubules diameter in the testis of patients with cryptorchidism, to verify if the previous use of human chorionic gonadotrophin (hCG) affects these structures.
  49. ncbi Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis
    Baris Altay
    Urology Department, Ege University Medical School, Bornova, Izmir, Turkey
    Fertil Steril 80:828-31. 2003
    ..To examine the histopathological effects of diabetes on spermatogenesis and to detect the proliferation of germ cells with proliferative cell nuclear antigen (PCNA)...
  50. ncbi Altered expression of connexins 26 and 43 in Sertoli cells in seminiferous tubules infiltrated with carcinoma-in-situ or seminoma
    Ralph Brehm
    Institute of Veterinary Anatomy, University of Giessen, Germany
    J Pathol 197:647-53. 2002
    ....
  51. ncbi Potency of testicular somatic environment to support spermatogenesis in XX/Sry transgenic male mice
    Mayuko Ishii
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1 1 1, Tokyo, Japan
    Development 134:449-54. 2007
    ..Microarray analysis of seminiferous tubules isolated from XX/Sry testes confirmed the missing expression of several Y-linked genes and the alterations ..
  52. ncbi Estrogenic induction of spermatogenesis in the hypogonadal mouse
    F J Ebling
    School of Biomedical Sciences, University of Nottingham Medical School, Queen s Medical Centre, United Kingdom
    Endocrinology 141:2861-9. 2000
    ..The identification of effects of estradiol on testicular function provides a conceptual basis to reexamine the speculative link between increased exposure to environmental estrogens and reduced fertility in man...
  53. ncbi 27 kDa extracellular matrix protein revealed by a monoclonal antibody raised against rat testis
    F Yazama
    Department of Anatomy, Yokohama City University, School of Medicine, Yokohama, Kanagawa 236 0004, Japan
    Anat Rec 260:402-9. 2000
    ..From these data, the existence of a testis specific 27 kDa basement membrane protein, which associate with type IV collagen and laminin, was suggested...
  54. ncbi Effects of 2-bromopropane on spermatogenesis in the Sprague-Dawley rat
    H Y Son
    Screening and Toxicology Research Center, Korea Research Institute of Chemical Technology, Taejon, Republic of Korea
    Reprod Toxicol 13:179-87. 1999
    ..Degeneration of spermatogonia was observed during Stages I-IV in seminiferous tubules on Day 1 after treatment...
  55. ncbi Transplantation of male germ line stem cells restores fertility in infertile mice
    T Ogawa
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3850 Baltimore Ave, Philadelphia, Pennsylvania 19104, USA
    Nat Med 6:29-34. 2000
    ..Thus, transplantation of spermatogonial stem cells from an infertile donor to a permissive testicular environment can restore fertility and result in progeny with the genetic makeup of the infertile donor male...
  56. ncbi The contractile phenotype of peritubular smooth muscle cells is locally controlled: possible implications in male fertility
    Francesca Romano
    Department of Histology and Medical Embryology, University of Rome La Sapienza, 00161 Rome, Italy
    Contraception 72:294-7. 2005
    ..activity of peritubular tissue is responsible for the propulsion of spermatozoa along the lumen of seminiferous tubules toward the hilum of the testis...
  57. ncbi Qualitative and quantitative decline in spermatogenesis of the follicle-stimulating hormone receptor knockout (FORKO) mouse
    H Krishnamurthy
    The Molecular Reproduction Research Laboratory, Clinical Research Institute of Montreal, Montreal, Quebec, Canada H2W 1R7
    Biol Reprod 62:1146-59. 2000
    ..These data allow us to conclude that genetic disruption of FSH receptor signaling in the rodent induces major changes that might contribute to reduced fertility...
  58. ncbi Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
    Taiza Stumpp
    Laboratory of Embryology, Department of Morphology, Federal University of Sao Paulo, Sao Paulo, Brazil
    Anat Rec A Discov Mol Cell Evol Biol 279:611-22. 2004
    ..Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. 2004...
  59. ncbi Histochemical analysis of rat testicular glycoconjugates. 1. Subsets of N-linked saccharides in seminiferous tubules
    C J Jones
    Department of Pathological Sciences, University of Manchester, UK
    Histochem J 24:319-26. 1992
    ..Sertoli cell surfaces and cytoplasmic granules showed a high level of N-glycan expression...
  60. ncbi Pituitary adenylate cyclase-activating polypeptide gene expression regulated by a testis-specific promoter in germ cells during spermatogenesis
    P B Daniel
    Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Howard Hughes Medical Institute, and Harvard Medical School, Boston 02114, USA
    Endocrinology 141:1218-27. 2000
    ..of PACAP gene expression during the spermatogenic cycle, accomplished by RT-PCR of segments of isolated seminiferous tubules, identified intense expression in the postmeiotic round spermatids during developmental stages I-VIII...
  61. ncbi Murine germ cells do not require functional androgen receptors to complete spermatogenesis following spermatogonial stem cell transplantation
    D S Johnston
    School of Molecular Biosciences and Center for Reproductive Biology, Washington State University, Pullman 99164, USA
    Endocrinology 142:2405-8. 2001
    ..Testicular cells from testicular feminized mice were injected into the seminiferous tubules of azoospermic mice expressing functional androgen receptors...
  62. ncbi Cellular and regional distributions of ubiquitin-proteasome and endocytotic pathway components in the epithelium of rat efferent ductules and initial segment of the epididymis
    Cleida A Oliveira
    Department of Morphology, Federal University of Minas Gerais, MG, Brazil
    J Androl 30:590-601. 2009
    ..Localization of ubiquitin-proteasome and endocytotic pathway components suggests that differential regulation is required for protein degradation and turnover in efferent ductules and head of the epididymis...
  63. pmc Do seminiferous tubules secrete a fluid rich in KHCO3?
    P Y D Wong
    Department of Physiology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
    J Physiol 542:335. 2002
  64. ncbi Inhibition of sperm production in mice by annexin V microinjected into seminiferous tubules: possible etiology of phagocytic clearance of apoptotic spermatogenic cells and male infertility
    Y Maeda
    Department of Urology, School of Medicine, Kanazawa University, Takara machi, Kanazawa, Ishikawa 920 0934, Japan
    Cell Death Differ 9:742-9. 2002
    ..For this purpose, the PS-binding protein annexin V was microinjected into the seminiferous tubules of normal live mice, and their testes were examined...
  65. ncbi Immunolocalization of aromatase in stallion Leydig cells and seminiferous tubules
    Herbert Sipahutar
    EA2608, Laboratoire de Biochimie et de Biologie Moléculaire Université de Caen, Caen, France
    J Histochem Cytochem 51:311-8. 2003
    ..The present study in stallions indicates that in addition to Leydig cells, Sertoli cells also appear to participate in estrogen synthesis, and this could play a paracrine role in the regulation of spermatogenesis...
  66. ncbi Cell death induced by zinc and cadmium is mediated by clusterin in cultured mouse seminiferous tubules
    Tehila Kaisman-Elbaz
    Department of Morphology, Zlotowski Center for Neuroscience, Ben Gurion University of the Negev, Beer Sheva, Israel
    J Cell Physiol 220:222-9. 2009
    Sertoli cells, lining the walls of the seminiferous tubules, are in close contact with and regulate all aspects of the development of the germ cells...
  67. ncbi Two distinct localization patterns of testis-specific serine protease 1 (TESSP1) in the seminiferous tubules of the mouse testis
    Naoharu Takano
    Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan
    Zoolog Sci 26:294-300. 2009
    ..the wild-type mouse testis, dot-like signals for TESSP1 were observed in the adluminal compartment of the seminiferous tubules, while diffused signals were found in the basal compartment...
  68. pmc Impaired male fertility and atrophy of seminiferous tubules caused by haploinsufficiency for Foxa3
    Rüdiger Behr
    Department of Genetics, University of Pennsylvania Medical School, 415 Curie Boulevard, Philadelphia, PA 19104 6145, USA
    Dev Biol 306:636-45. 2007
    ..In summary, we have identified Foxa3 as a transcriptional regulator with a dominant phenotype in germ cell maintenance and suggest FOXA3 as a potential candidate gene for subfertility in man...
  69. ncbi Involution of seminiferous tubules in aged hamsters: an ultrastructural, immunohistochemical and quantitative morphological study
    E Morales
    Department of Cellular Biology, Medical School, University of Murcia, Spain
    Histol Histopathol 19:445-55. 2004
    In this study, we examined the age-related changes on morphometric parameters and ultrastructure of seminiferous tubules, and on the expression of extracellular matrix proteins in lamina propria of Syrian hamsters...
  70. pmc Morphological evidences indicate that the interference of cimetidine on the peritubular components is responsible for detachment and apoptosis of Sertoli cells
    Estela Sasso-Cerri
    Laboratory of Histology and Embryology Department of Morphology, Dental School of São Paulo State University UNESP, Araraquara, Sao Paulo, Brazil
    Reprod Biol Endocrinol 6:18. 2008
    Cimetidine, referred as antiandrogenic agent, has caused alterations in the seminiferous tubules, including alterations in the peritubular tissue and death of myoid cells by apoptosis...
  71. pmc Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene
    K M Robertson
    Prince Henry s Institute of Medical Research, Monash Medical Centre, Clayton, Victoria 3168, Australia
    Proc Natl Acad Sci U S A 96:7986-91. 1999
    ..Our findings indicate that local expression of aromatase is essential for spermatogenesis and provide evidence for a direct action of estrogen on male germ cell development and thus fertility...
  72. ncbi Delay in sexual maturity of the follicle-stimulating hormone receptor knockout male mouse
    H Krishnamurthy
    The Molecular Reproduction Research Laboratory, Clinical Research Institute of Montreal, Montreal, Quebec, Canada H2W 1R7
    Biol Reprod 65:522-31. 2001
    ..The drastic reduction in testicular weight and shrinkage of seminiferous tubules that occurred at this early age persisted into the adult stage in the FORKOs, suggesting inhibition of the ..
  73. ncbi Detection of germ cell-derived proteins in testicular interstitial fluid: potential for monitoring spermatogenesis in vivo
    K J Turner
    MRC Reproductive Biology Unit, Edinburgh, Scotland
    J Androl 17:127-36. 1996
    The aim of the present study was to assess whether proteins secreted by the seminiferous tubules (ST) can be detected in testicular interstitial fluid (IF) and testicular (TV), spermatic (SV), and peripheral venous (PV) plasma from adult ..
  74. pmc Apoptosis during the seasonal spermatogenic cycle of Rana catesbeiana
    Estela Sasso-Cerri
    Department of Morphology, Dental School, Sao Paulo State University UNESP, Araraquara, Brazil
    J Anat 209:21-9. 2006
    ..Thus, apoptosis may participate not only in the maintenance of spermatogenic homeostasis, but also in the cyclical control of the different spermatogenic processes according to seasonal changes of the testicular compartments as a whole...
  75. ncbi Morphological alterations and intratubular lipid inclusions as indicative of spermatogenic damage in cimetidine-treated rats
    E Sasso-Cerri
    Department of Morphology, Federal University of Sao Paulo, Brazil
    Arch Androl 46:5-13. 2001
    ..Besides plasma testosterone levels, morphological and morphometric aspects of the seminiferous tubules as well as histochemical analysis of the lipid content by oil red O were emphasized...
  76. ncbi The effects of subacute inhalation of di (2-ethylhexyl) phthalate (DEHP) on the testes of prepubertal Wistar rats
    Norie Kurahashi
    Department of Public Health, Hokkaido University Graduate School of Medicine, Japan
    J Occup Health 47:437-44. 2005
    ..Our study showed that inhaled DEHP increased plasma testosterone concentrations in prepubertal rats and suggested that their effects were more sensitive to inhalation of DEHP than oral dosing...
  77. ncbi Localization of dynamin 2 in rat seminiferous tubules during the spermatogenic cycle
    Hiroki Iguchi
    Department of Urology, Okayama University Graduate School of Medicine and Dentistry, Japan
    Acta Med Okayama 56:205-9. 2002
    ..Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats...
  78. ncbi Study of spermatogenesis and thickening of lamina propria in the human seminiferous tubules
    Yoko Sato
    Department of Urology, St Marianna University School of Medicine, Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Sugao Miyamae, Kawasaki, Japan
    Fertil Steril 90:1310-2. 2008
    The negative correlation between the thickness of the lamina propria and Johnsen's score count in individual seminiferous tubules was observed and the tubules with thin lamina propria indicated almost complete spermatogenesis...
  79. pmc GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules
    Yong Wang
    State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China
    BMC Cell Biol 10:25. 2009
    ..GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids ..
  80. ncbi In situ demonstration of both TUNEL-labeled germ cell and Sertoli cell in the cimetidine-treated rats
    E Sasso-Cerri
    Department of Morphology, Federal University of Sao Paulo, Sao Paulo, Brazil
    Histol Histopathol 17:411-7. 2002
    ..Besides germ cells, TUNEL-positive Sertoli cells were also found in the injured seminiferous tubules. Thus, a relationship between dying germ cells and Sertoli cell damage and/or death must be considered in ..
  81. ncbi Long-term proliferation in culture and germline transmission of mouse male germline stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Japan
    Biol Reprod 69:612-6. 2003
    ..and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has ..
  82. ncbi Somatostatin inhibits stem cell factor messenger RNA expression by Sertoli cells and stem cell factor-induced DNA synthesis in isolated seminiferous tubules
    I Goddard
    , , F-69921 Oullins, France
    Biol Reprod 65:1732-42. 2001
    ..data is supported by an SRIF-mediated decrease in SCF-induced incorporation of [(3)H]thymidine in isolated seminiferous tubules. In situ hybridization and confocal microscopy show that, in seminiferous tubules only, spermatogonia ..
  83. ncbi Cell-cycle inhibitors p27Kip1 and p21Cip1 regulate murine Sertoli cell proliferation
    Denise R Holsberger
    Department of Veterinary Biosciences, University of Illinois, Urbana 61802, USA
    Biol Reprod 72:1429-36. 2005
    ..Furthermore, loss of both CDKIs causes additive effects on DSP and testis weight, suggesting a central role for these CDKIs in testis development...
  84. ncbi Spermiogenesis and chromatin condensation in the common tree shrew, Tupaia glis
    Worawit Suphamungmee
    Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand
    Cell Tissue Res 331:687-99. 2008
    ..Cross-immunoreactivity of T. glis H3 and protamine to those of primates suggests the evolutionary resemblance of these nuclear basic proteins in primate germ cells...
  85. ncbi Immunohistochemical study of flotillin-1 in the rat testis during postnatal development
    Heechul Kim
    Department of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju 690 756, South Korea
    Acta Histochem 110:224-31. 2008
    ..These results suggest that flotillin-1 participates in the developmental process of Sertoli cells and is involved in the regulation of spermatogenesis...
  86. ncbi Abnormal sperm morphology caused by defects in Sertoli cells of Cnot7 knockout mice
    Takehiko Ogawa
    Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama, Japan
    Arch Histol Cytol 67:307-14. 2004
    ..These results support our previous report that Sertoli cell defects in the Cnot7-/- testes could induce oligo-astheno-teratozoospermia...
  87. ncbi Hepatocyte growth factor modulates Sertoli-Sertoli tight junction dynamics
    A Catizone
    Department of Histology and Medical Embryology, School of Medicine, University of Rome Sapienza, Rome, Italy
    J Cell Physiol 216:253-60. 2008
    ..In conclusion we report that in the testis HGF regulates Sertoli-Sertoli tight junctions inducing a reduction and redistribution of occludin possibly modulating the levels of uPA and active TGFbeta...
  88. ncbi Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis
    Yanlong Zhang
    Department of Molecular Genetics, Kochi University Medical School, Kochi 783 8505, Japan
    Glycobiology 15:649-54. 2005
    ..analysis, in which wild-type spermatogonia expressing the green fluorescent protein were injected into the seminiferous tubules of CST-null testis...
  89. ncbi Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis
    Shinichiro Chuma
    Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Development 132:117-22. 2005
    ..show that epiblast cells and PGCs can establish colonies of spermatogenesis after transfer into postnatal seminiferous tubules of surrogate infertile mice...
  90. ncbi Lonidamine affects testicular steroid hormones in immature mice
    Maria Elsa Traina
    Department of Drug Research and Evaluation, Istituto Superiore di Sanita, Viale Regina Elena, 299 00161 Rome, Italy
    Toxicol Appl Pharmacol 221:95-101. 2007
    ....
  91. ncbi Effect of divergent selection for testosterone production on testicular morphology and daily sperm production in boars
    S Walker
    Department of Animal Science, North Carolina State University, Raleigh 27695 7621, USA
    J Anim Sci 82:2259-63. 2004
    ..Volume densities for Leydig cells, seminiferous tubules, and Sertoli cells were estimated along with sperm production...
  92. ncbi Effect of methotrexate (MTX) administration on spermatogenesis: an experimental on animal model
    S Shrestha
    Department of Anatomy, B P Koirala Institute of Health Sciences, Dharan, Nepal
    Nepal Med Coll J 9:230-3. 2007
    ..Further microscopic study of seminiferous tubules, interstitial spaces, primary spermatocytes and spermatids were carriedout...
  93. ncbi 2,5-hexanedione and carbendazim coexposure synergistically disrupts rat spermatogenesis despite opposing molecular effects on microtubules
    Robert J Markelewicz
    Department of Pathology and Laboratory Medicine, Brown University, 175 Meeting Street, Providence, Rhode Island 02912, USA
    Toxicol Sci 80:92-100. 2004
    ....
  94. ncbi Impaired reproduction in transgenic mice overexpressing Gamma-aminobutyric acid transporter I (GAT1)
    Jia Hua Hu
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Cell Res 14:54-9. 2004
    ..Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment...
  95. ncbi Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere
    Adelina Ferreira
    Departamento de Biologia Celular, Universidade Estadual de Campinas, SP, Brazil
    Biocell 27:353-62. 2003
    ..Comparisons between Tropiduridae and other lizard families are made...
  96. ncbi Morphological study of the spermatogenesis in the teleost Piaractus mesopotamicus
    Carminda da Cruz-Landim
    Departamento de Biologia, Instituto de Biociencias, Universidade Estadual Paulista, Rio Claro, SP, Brazil
    Biocell 27:319-28. 2003
    ..The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type in fishes...
  97. ncbi Spermatogenesis and plasma testosterone levels in Western Australian burrowing desert frogs, Cyclorana platycephala, Cyclorana maini, and Neobatrachus sutor, during aestivation
    A G Shalan
    School of Animal Biology and Centre for Native Animal Research, The University of Western Australia, Perth, WA 6009, Australia
    Gen Comp Endocrinol 136:90-100. 2004
    ..Changes in plasma testosterone levels correlated significantly with variations in the diameter of the seminiferous tubules and the GSI, suggesting that this hormone plays a major role in controlling testicular recrudescence in ..
  98. ncbi Effects of vasectomy on spermatogenesis and fertility outcome after testicular sperm extraction combined with ICSI
    C M McVicar
    Obstetrics and Gynaecology, School of Medicine, Queen s University Belfast, Institute of Clinical Science, UK
    Hum Reprod 20:2795-800. 2005
    ..The study aims were to compare sperm yields of men post-vasectomy or with obstructive azoospermia (OA) of unknown aetiology with yields of fertile men and to assess any alteration in the clinical pregnancy rates after ICSI...
  99. ncbi Morphological and morphometric study on the effect of simulated microgravity on rat testis
    M A H Motabagani
    Department of Anatomy, College of Medicine, King Faisal University, Dammam, Kingdom of Saudi Arabia
    Chin J Physiol 50:199-209. 2007
    ..Histologically, few abnormalities were observed in some seminiferous tubules in one-week hindlimb-suspended group...
  100. ncbi Maternal vitamin B12 deficiency affects spermatogenesis at the embryonic and immature stages in rats
    Toshiaki Watanabe
    Department of Environment for Life and Living, School of Human Science and Environment, Himeji Institute of Technology, University of Hyogo, Shinzaike Honcho 1 1 12, Himeji, Japan
    Congenit Anom (Kyoto) 47:9-15. 2007
    ..The incidence of seminiferous tubules having apoptotic cells was 51.5% in the VB12-D group...
  101. ncbi Expression of the adaptor protein m-Numb in mouse male germ cells
    Serena Corallini
    Dipartimento di Istologia ed Embriologia Medica, , Via Antonio Scarpa 14, 00161 Rome, Italy
    Reproduction 132:887-97. 2006
    ..Finally, we employed confocal immunofluorescence microscopy of whole mount seminiferous tubules and isolated germ cells to gain more insight into the subcellular localization of m-Numb...

Research Grants62

  1. Male germline stem cell culture and genetic modification
    RALPH BRINSTER; Fiscal Year: 2007
    ..The ability of these cells to colonize the seminiferous tubules of recipient animals and generate spermatogenesis as well as their ability to differentiate in vitro will ..
  2. Drug Transport at the Blood-Testis Barrier
    Nathan J Cherrington; Fiscal Year: 2013
    ..As the major component of the blood-testis barrier, Sertoli cells separate the adluminal compartment of the seminiferous tubules (STs) from the rest of the body by the formation of tight junctions below developing germ cells...
  3. BDADs for Male Contraception
    John K Amory; Fiscal Year: 2013
    ..Within the seminiferous tubules of the testes, a testes-specific aldehyde dehydrogenase called ALDH1a2 biosynthesizes retinoic acid, a ..
  4. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
    ..Because of evolutionary distance between rhesus and mouse, rhesus SSCs will engraft and persist in mouse seminiferous tubules, but will not produce complete spermatogenesis...
  5. Epigenetic Reprogramming in Germline by Phthalates
    Kwan Hee Kim; Fiscal Year: 2013
    ..di(2- ethylhexyl) phthalate (DEHP), the most abundant phthalate, caused disorganization of germ cells in seminiferous tubules, changes in the distribution of spermatogenic stages, increased apoptosis, increased number of ..
  6. Nongenomic Androgen Signaling in Sertolli Cells
    William H Walker; Fiscal Year: 2010
    ..is to determine whether non-classical testosterone actions are required to release mature spermatozoa from seminiferous tubules. We will determine whether testosterone activates Src and ERK kinases in cultured seminiferous tubules...
  7. Genetic analysis of male gonadal development
    Mary C Mullins; Fiscal Year: 2013
    ..acting in the morphogenesis of the somatic and germ cell tissue into testis cords and differentiated seminiferous tubules, or the mechanisms regulating the distinctive spermatogonial cell division program and early ..
  8. SERTOLI CELL-GERM CELL SURFACE MEMBRANE INTERACTIONS
    ROBERT DEPHILIP; Fiscal Year: 1991
    ..the temporal and spatial expression of Sertoli cell surface antigens in developing and in mature, cycling seminiferous tubules; and (4) the identification of Sertoli cell surface antigens that are involved in either the binding of ..
  9. MECHANISMS OF TOXICITY IN TESTES
    Michael Welsh; Fiscal Year: 1999
    ..each phosphorylated isoform of hsp27 and localize each isoform in frozen sections of testis and in seminiferous tubules, using confocal microscopy...
  10. Regulation of Gene Expression During Spermatogenesis
    Peter Petrusz; Fiscal Year: 2004
    ..is the reduction in the concentration of bioavailable androgens in the peritubular compartment and the seminiferous tubules. DNA microarray technology offers a novel and powerful means to analyze gene expression in a comprehensive, ..
  11. A New Approach to Generate Transgenic Animals
    Martin Dym; Fiscal Year: 2004
    ..it has very high potential to produce mature functional sperm after transplantation into recipient sterile seminiferous tubules and then the transfer of a modified genome to offspring...
  12. REGULATION OF SPERMATOGENESIS IN MAMMALS
    Barry Zirkin; Fiscal Year: 1991
    ..response of specific spermatogenic cells to defined changes in testosterone concentration within the seminiferous tubules of the adult rat. Two specific aims are proposed...
  13. ROLE OF PROTEIN-TYROSINE KINASES IN SPERMATOGENESIS
    Steven Hanks; Fiscal Year: 1993
    ..Interactions between germ cells and Sertoli cells in the seminiferous tubules are crucial for sperm development, but little has been learned to date about how these interactions are ..
  14. CHARACTERIZATION OF A GHRH-LIKE SUBSTANCE IN RAT TESTIS
    ORA PESCOVITZ; Fiscal Year: 1992
    ..a blood-testis barrier that restricts the movement of many dyes, steroids and peptides into and out of the seminiferous tubules. Several neuropeptides that were originally considered to be restricted to the hypothalamus or the ..
  15. ABP AND THE REGULATION OF GERM CELL APOPTOSIS IN TESTIS
    Peter Petrusz; Fiscal Year: 2000
    ..proliferation and degeneration (apoptosis) of germ cells by changing the steroid hormone milieu within the seminiferous tubules. This may involve not only testosterone, but also is biologically active intratesticular metabolites ..
  16. Constitutively active LH receptors in transgenic mice
    Prema Narayan; Fiscal Year: 2007
    ..increased prepubertal levels of testosterone, accompanied by decreased testicular size and diameter of the seminiferous tubules. Female transgenic mice exhibit increased folliculogenesis at early ages, and at three months there is ..
  17. REGULATION OF TESTICULAR BLOOD FLOW
    Claude Desjardins; Fiscal Year: 1993
    ..demand coincide with mitotic and/or meiotic events that occur within well-defined segments (2-6 mm) of seminiferous tubules. We will test the proposition that vascular regulatory processes intrinsic to the testis directly control ..
  18. RETINOL-MEDICATED REGULATION OF SPERMATOGENESIS
    Kwan Kim; Fiscal Year: 1993
    ..The retinol-induced testes reinitiate the spermatogenic process in a synchronized manner all along the seminiferous tubules. It is Presumed that sPecific gene Products and mRNAs are synthesized in direct response to retinol in ..
  19. SPERMATOGONIAL STEM CELL CULTURE AND TRANSPLANTATION
    RALPH BRINSTER; Fiscal Year: 2002
    We have developed a method that allows transfer of testicular cells from a fertile male to the seminiferous tubules of an infertile male...
  20. Optimizing Testis Transplantation to Study RET Signaling
    Cathy Naughton; Fiscal Year: 2005
    ..However, transplant efficiency of the donor testicle is not perfect as not all immature germ cells in the seminiferous tubules display complete maturation to spermatozoa...
  21. Role of VEGF in Testis Development and Function
    ANDREA CUPP; Fiscal Year: 2004
    Formation of the seminiferous cords, the precursor seminiferous tubules, is the first morphological event that distinguishes the testis from the ovary. The cords contain Sertoli and germ cells surrounded by peritubular cells...
  22. PRIMORDIAL GERM CELL MIGRATION IN THE EMBRYO
    Christopher Wylie; Fiscal Year: 2003
    ..These are the forerunners of the seminiferous tubules in males, and the ovarian follicles in females...
  23. FATE OF SPERMATOGONIAL STEM CELLS
    Martin Dym; Fiscal Year: 2007
    ..In the third aim, we will examine gene expression in isolated type A spermatogonia and in seminiferous tubules in culture using serial analysis of gene expression (SAGE) and DNA array procedures in response to ligands...
  24. REGULATION OF TESTIS GENE EXPRESSION BY CAMP AND CREB
    William Walker; Fiscal Year: 2002
    ..tested is that alterations in the relative concentrations of activator and repressor CREB isoforms in the seminiferous tubules regulate the expression of cAMP-inducible genes required for the expansion and differentiation of male germ ..
  25. Stem Cell Function in the Aging Testis
    Stefan Schlatt; Fiscal Year: 2006
    ..current project is to determine an age-dependent (in) ability of testicular stem cells to recolonize the seminiferous tubules and to restore spermatogenesis after germ cell depletion in mice and monkeys...
  26. BASEMENT MEMBRANE REGULATION OF SERTOLI CELLS
    Martin Dym; Fiscal Year: 2000
    ..from the Investigator's Abstract): This application proposes that basement membrane components of the seminiferous tubules play a determining role in Sertoli cell function and in the formation of a tubular lumen by a selective ..
  27. REGULATION OF TESTIS GENE EXPRESSION BY CAMP AND CREB
    William Walker; Fiscal Year: 2001
    ..tested is that alterations in the relative concentrations of activator and repressor CREB isoforms in the seminiferous tubules regulate the expression of cAMP-inducible genes required for the expansion and differentiation of male germ ..
  28. NOVEL CONTRACEPTIVES WITH ANTI-HIV ACTIVITY
    OSMOND D CRUZ; Fiscal Year: 2001
    ..The preclinical data on in vivo efficacies of WHI-05 and WHI-07 will be essential to further explore the utility of these novel drugs for clinical studies in human patients. ..
  29. Vanadocenes as a New Class of Spermicidal Drugs
    OSMOND D CRUZ; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  30. NUCLEOPROTEINS IN MAMMALIAN SPERMATOGENESIS
    Marvin Meistrich; Fiscal Year: 2006
    ..Thus they provide a well-defined model system for study of human infertility. ..
  31. Stampidine: A Novel Broad-Spectrum Anti-HIV Microbicide
    OSMOND D CRUZ; Fiscal Year: 2005
    ..Specific Aims 1 and 2 may provide the foundation for the clinical development of STAMP in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
  32. Multidisciplinary Program in Female and Male Reproduction
    EUGENE ALBRECHT; Fiscal Year: 2008
    ..The discoveries obtained in the baboon primate model are expected to translate to the human to advance knowledge of human reproductive biology. [unreadable] [unreadable]..
  33. Phase II SBIR: WHI-07: A Novel Dual-Function Microbicide
    OSMOND D CRUZ; Fiscal Year: 2005
    ....
  34. PHI-443: Novel Non-Spermicidal Anti-HIV Microbicide
    OSMOND D CRUZ; Fiscal Year: 2003
    ..Specific Aims 1 and 2 may provide the foundation for the clinical development of PHI-443 in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
  35. PHI 346: A Novel Dual-Function Microbicide
    OSMOND D CRUZ; Fiscal Year: 2003
    ..Following successful completion of the Phase I in vivo efficacy studies, gel formulation of PHI-346 will be further explored as a vaginal dual-function microbicide in Phase II. ..
  36. FLP-102: Rationally Engineered Antiviral Protein
    OSMOND D CRUZ; Fiscal Year: 2003
    ..The preclinical data on FLP-102 will be essential to further explore the utility of this novel recombinant PAP mutant for Phase II. ..
  37. METVAN: A Novel Anticancer Agent
    OSMOND D CRUZ; Fiscal Year: 2003
    ..The knowledge gained from these studies described under Specific Aims 1-2 is expected to facilitate the design of innovative treatment regimens employing METVAN for metastatic breast cancer and brain tumor patients. ..
  38. TOXICOLOGY FOR TEACHERS
    Ann de Peyster; Fiscal Year: 2003
    ..These workshops are an innovative way for scientists and teachers to work together to improve science education. ..
  39. ENVIRONMENTAL/INDUSTRIAL TOXICANTS AND TESTICULAR INJURY
    Kim Boekelheide; Fiscal Year: 2003
    ..Together, these complimentary approaches will elucidate the key molecular mechanisms responsible for irreversible testicular injury. ..
  40. NEURAL REGULATION OF CIRCADIAN RHYTHMS AND PHOTOPERIODIS
    ERIC BITTMAN; Fiscal Year: 2003
    ..Our results will have applications to human pathologies including jet lag, sleep disorders, seasonal depression, and infertility. ..
  41. Sertoli Cell-Treated Umbilical Cord Blood for Stroke
    Don Cameron; Fiscal Year: 2003
    ..The success of directing and protecting stem cells obtained from umbilical cord blood into a neural phenotype (i.e. SCT-441) has important implications for the treatment of stroke in humans. ..
  42. An in vivo model to study blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  43. Germ Cell Aging
    CHRISTI WALTER; Fiscal Year: 2007
    ..This study will advance our understanding of the fundamental mechanisms involved in male germline integrity. ..
  44. Calorie Restriction Effects on Chromosomal Aberrations
    CHARLEEN MOORE; Fiscal Year: 2003
    ..The results will be essential in identifying potential mechanisms by which CR acts in cancer prevention and delay of the aging process in long-lived species such as the rhesus monkey and humans. ..
  45. Sertoli Cell Mediated Engulfment of Apoptotic Germ Cells
    Kodi S Ravichandran; Fiscal Year: 2010
    ..abstract_text> ..
  46. Base Excision Repair, Genetic Integrity & Health Span
    CHRISTI WALTER; Fiscal Year: 2008
    ..Genetic integrity, apoptosis, and pathology will be examined using a variety of molecular, biochemical and cell biological approaches. ..
  47. GERM CELLS--DNA REPAIR, MUTATION, & ENVIRONMENTAL AGENTS
    CHRISTI WALTER; Fiscal Year: 2001
    ..This study will advance an understanding of the fundamental mechanisms involved in mutagenesis in germ cells. ..
  48. Junction Dynamics and Male Fertility Regulation
    C Yan Cheng; Fiscal Year: 2008
    ..In summary, these studies not only will define the regulation of AJ dynamics, they will identify new leads and innovative approaches for male contraceptive development. [unreadable] [unreadable]..
  49. Metal Toxicant Effects-Small Heat-Shock Protein Function
    Michael Welsh; Fiscal Year: 2005
    ..It is expected that these studies will contribute significantly to our understanding of sublethal effects of toxic metals (either singly or in combinations) and the function of sHSPs in muscle and kidney. ..
  50. Annual SMRU Traveling Scholar Conference
    Susan Benoff; Fiscal Year: 2005
    ..Without this exposure, many of them would have likely pursued different career paths. ..
  51. Autoimmune Oophoritis: Consequences of Gamete Vaccines
    Kenneth Tung; Fiscal Year: 2006
    ..Specifically, we will determine the immunogenetic regulation of the immune response to CP2, and the influence of the B cell epitope specificity of the ZP Ab on the induction of progenic AOD. ..
  52. INTEGRATING ENVIRONMENTAL HEALTH SCIENCE IN RURAL SCHOOL
    Larry Johnson; Fiscal Year: 2006
    ..abstract_text> ..
  53. Testicular Torsion: Mechanisms and Treatment
    Terry Turner; Fiscal Year: 2005
    ..It is likely that these investigations will be relevant to other forms of oxidative stress inducing testicular dysfunction. ..
  54. SERTOLI CELL TOXICITY IN VIVO--MICROTUBULE DISRUPTION
    Kim Boekelheide; Fiscal Year: 2001
    ..Together, these complimentary approaches will critically examine key steps in the pathogenetic sequence by which microtubule disrupters alter the supportive capacity of Sertoli cells resulting in loss of germ cells and infertility. ..
  55. PHTHALATE-INDUCED MURINE TESTICULAR DYSGENESIS AND P53
    Kim Boekelheide; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  56. Regulation of spermatogonial stem cells by vitamin A
    Derek McLean; Fiscal Year: 2007
    ..The current proposal is novel because this is the first time that the effect of a factor, such as vitamin A, on SSC differentiation has been evaluated. [unreadable] [unreadable] [unreadable]..
  57. NUTRITIONAL AND METABOLIC SIGNIFICANCE OF SELENIUM
    Raymond Burk; Fiscal Year: 2003
    ..These and other studies are proposed to evaluate the role of Se-P in protecting endothelial cells (and other cells) against oxidant substances in the extracellular space. ..
  58. Annual SMRU Traveling Scholars Conference
    Susan Benoff; Fiscal Year: 2006
    ..Without this exposure, many of them would have likely pursued different career paths. [unreadable] [unreadable] [unreadable] [unreadable]..
  59. Insulin and Oocyte Chromatin Segregation
    Gary Smith; Fiscal Year: 2009
    ..abstract_text> ..