testis

Summary

Summary: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Top Publications

  1. ncbi A novel class of small RNAs bind to MILI protein in mouse testes
    Alexei Aravin
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 442:203-7. 2006
  2. ncbi Essential role of Plzf in maintenance of spermatogonial stem cells
    Jose A Costoya
    Cancer Biology and Genetics Program, Department of Pathology Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Genet 36:653-9. 2004
  3. ncbi Plzf is required in adult male germ cells for stem cell self-renewal
    F William Buaas
    Department of Genome Sciences, Box 357730, 1705 NE Pacific Street, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nat Genet 36:647-52. 2004
  4. doi Cancer/testis (CT) antigens: potential targets for immunotherapy
    Otavia L Caballero
    Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan Kettering Cancer Center, New York City, USA
    Cancer Sci 100:2014-21. 2009
  5. ncbi An abundance of X-linked genes expressed in spermatogonia
    P J Wang
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Genet 27:422-6. 2001
  6. pmc DMRT1 prevents female reprogramming in the postnatal mammalian testis
    Clinton K Matson
    Developmental Biology Center and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nature 476:101-4. 2011
  7. ncbi The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis
    James E Shima
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
    Biol Reprod 71:319-30. 2004
  8. pmc Genome-wide analysis of cancer/testis gene expression
    Oliver Hofmann
    Department of Biostatistics, Harvard School of Public Health, 655 Huntington Avenue, SPH2, 4th Floor, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:20422-7. 2008
  9. doi The avian Z-linked gene DMRT1 is required for male sex determination in the chicken
    Craig A Smith
    Murdoch Children s Research Institute and Department of Paediatrics, The University of Melbourne, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    Nature 461:267-71. 2009
  10. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000

Research Grants

  1. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
  2. Yun Fai Chris Lau; Fiscal Year: 2014
  3. Ming Zhou; Fiscal Year: 2014
  4. John H Postlethwait; Fiscal Year: 2016
  5. Interdisciplinary Research Training in Reproduction for Indian Fellows
    John C Herr; Fiscal Year: 2010
  6. Regulation of insulins by the Rhox5 homeobox gene supports spermatogenesis
    James Maclean; Fiscal Year: 2009
  7. Lois B Travis; Fiscal Year: 2016
  8. Ameae M Walker; Fiscal Year: 2015
  9. Sienmi Du; Fiscal Year: 2014
  10. Erythropoietin in Non-Erythroid Cells: Function and Regulation
    Josef T Prchal; Fiscal Year: 2013

Detail Information

Publications774 found, 100 shown here

  1. ncbi A novel class of small RNAs bind to MILI protein in mouse testes
    Alexei Aravin
    Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, New York 10021, USA
    Nature 442:203-7. 2006
    ..The identification of this new class of small RNAs provides an important starting point to determine the molecular function of Piwi proteins in mammalian spermatogenesis...
  2. ncbi Essential role of Plzf in maintenance of spermatogonial stem cells
    Jose A Costoya
    Cancer Biology and Genetics Program, Department of Pathology Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nat Genet 36:653-9. 2004
    Little is known of the molecular mechanisms whereby spermatogonia, mitotic germ cells of the testis, self-renew and differentiate into sperm...
  3. ncbi Plzf is required in adult male germ cells for stem cell self-renewal
    F William Buaas
    Department of Genome Sciences, Box 357730, 1705 NE Pacific Street, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nat Genet 36:647-52. 2004
    ..This is the first gene shown to be required in germ cells for stem cell self-renewal in mammals...
  4. doi Cancer/testis (CT) antigens: potential targets for immunotherapy
    Otavia L Caballero
    Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan Kettering Cancer Center, New York City, USA
    Cancer Sci 100:2014-21. 2009
    Cancer/testis (CT) antigens are protein antigens with normal expression restricted to adult testicular germ cells, and yet are aberrantly activated and expressed in a proportion of various types of human cancer...
  5. ncbi An abundance of X-linked genes expressed in spermatogonia
    P J Wang
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Genet 27:422-6. 2001
    Spermatogonia are the self-renewing, mitotic germ cells of the testis from which sperm arise by means of the differentiation pathway known as spermatogenesis...
  6. pmc DMRT1 prevents female reprogramming in the postnatal mammalian testis
    Clinton K Matson
    Developmental Biology Center and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nature 476:101-4. 2011
    ..Here we show that sexual fate is also surprisingly labile in the testis: loss of the DMRT1 transcription factor in mouse Sertoli cells, even in adults, activates Foxl2 and reprograms ..
  7. ncbi The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermatogenesis
    James E Shima
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
    Biol Reprod 71:319-30. 2004
    ..The testis provides an excellent model to determine the ability of microarrays to effectively characterize the changes in ..
  8. pmc Genome-wide analysis of cancer/testis gene expression
    Oliver Hofmann
    Department of Biostatistics, Harvard School of Public Health, 655 Huntington Avenue, SPH2, 4th Floor, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:20422-7. 2008
    Cancer/Testis (CT) genes, normally expressed in germ line cells but also activated in a wide range of cancer types, often encode antigens that are immunogenic in cancer patients, and present potential for use as biomarkers and targets for ..
  9. doi The avian Z-linked gene DMRT1 is required for male sex determination in the chicken
    Craig A Smith
    Murdoch Children s Research Institute and Department of Paediatrics, The University of Melbourne, Royal Children s Hospital, Melbourne, Victoria 3052, Australia
    Nature 461:267-71. 2009
    ..The feminized left gonad shows female-like histology, disorganized testis cords and a decline in the testicular marker, SOX9. The ovarian marker, aromatase, is ectopically activated...
  10. ncbi The mouse Spo11 gene is required for meiotic chromosome synapsis
    P J Romanienko
    Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health 20892, Bethesda, MD, USA
    Mol Cell 6:975-87. 2000
    ..We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis...
  11. ncbi Retinoid signaling determines germ cell fate in mice
    Josephine Bowles
    Division of Genetics and Developmental Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Science 312:596-600. 2006
    ..Meiosis is retarded in the fetal testis by the action of the retinoid-degrading enzyme CYP26B1, ultimately leading to spermatogenesis...
  12. pmc MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, 411 8540, Japan
    Dev Cell 20:364-75. 2011
    ..Our results indicate a conserved role for MITOPLD/Zuc in the piRNA pathway and link mitochondrial membrane metabolism/signaling to small RNA biogenesis...
  13. ncbi Male development of chromosomally female mice transgenic for Sry
    P Koopman
    Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, Mill Hill, London, UK
    Nature 351:117-21. 1991
    ..termed SRY in humans and Sry in mouse, has many of the genetic and biological properties expected of a Y-located testis-determining gene...
  14. ncbi Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9
    T Wagner
    Institute of Human Genetics, University of Freiburg, Federal Republic of Germany
    Cell 79:1111-20. 1994
    ..SOX9 encodes a putative transcription factor structurally related to the testis-determining factor SRY and is expressed in many adult tissues, and in fetal testis and skeletal tissue...
  15. ncbi Regulation of cell fate decision of undifferentiated spermatogonia by GDNF
    X Meng
    Research Programs of Developmental Biology, Molecular Neurobiology, Electron Microscopy Unit, Institute of Biotechnology, Viikki Biocenter, Finland
    Science 287:1489-93. 2000
    ..Nonmetastatic testicular tumors are regularly formed in older GDNF-overexpressing mice. Thus, GDNF contributes to paracrine regulation of spermatogonial self-renewal and differentiation...
  16. doi Sex determination involves synergistic action of SRY and SF1 on a specific Sox9 enhancer
    Ryohei Sekido
    Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nature 453:930-4. 2008
    ..male determinant as a result of the action of a single gene, Sry, whose role in sex determination is to initiate testis rather than ovary development from early bipotential gonads...
  17. pmc Paucity of genes on the Drosophila X chromosome showing male-biased expression
    Michael Parisi
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892 8028, USA
    Science 299:697-700. 2003
    ..These data indicate that the X chromosome is a disfavored location for genes selectively expressed in males...
  18. pmc Evolution in the fast lane: rapidly evolving sex-related genes in Drosophila
    Wilfried Haerty
    Department of Biology, McMaster University, Hamilton, Ontario L8S 4K1, Canada
    Genetics 177:1321-35. 2007
    ..Several testis-specific genes, male seminal fluid proteins (SFPs), and spermatogenesis genes show lineage-specific bursts of ..
  19. ncbi A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
    A H Sinclair
    Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, Lincoln s Inn Fields, London, UK
    Nature 346:240-4. 1990
    ..This gene is conserved and Y-specific among a wide range of mammals, and encodes a testis-specific transcript...
  20. ncbi Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
    Satomi Kuramochi-Miyagawa
    Department of Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita Shi, Osaka 565 0871, Japan
    Development 131:839-49. 2004
    ..These data indicate that MILI is essential for the differentiation of spermatocytes...
  21. ncbi Differentiation of the adult Leydig cell population in the postnatal testis
    S M Mendis-Handagama
    Department of Comparative Medicine, The University of Tennessee College of Veterinary Medicine, Knoxville, TN 37996, USA
    Biol Reprod 65:660-71. 2001
    ..onset of Leydig cell differentiation in the prepubertal rat as well as in the adult rat during repopulation of the testis interstitium after ethane dimethane sulfonate (EDS) treatment...
  22. doi Cellular source and mechanisms of high transcriptome complexity in the mammalian testis
    Magali Soumillon
    Center for Integrative Genomics, University of Lausanne, Genopode, CH 1015, Lausanne, Switzerland
    Cell Rep 3:2179-90. 2013
    ..using extensive RNA-seq data, we show that transcription of the genome is substantially more widespread in the testis than in other organs across representative mammals...
  23. pmc Sex chromosome-specific regulation in the Drosophila male germline but little evidence for chromosomal dosage compensation or meiotic inactivation
    Colin D Meiklejohn
    Department of Biology, University of Rochester, Rochester, New York, United States of America
    PLoS Biol 9:e1001126. 2011
    ..These findings help to resolve several previously conflicting reports and have implications for patterns of genome evolution and speciation in Drosophila...
  24. pmc Newer insights into premeiotic development of germ cells in adult human testis using Oct-4 as a stem cell marker
    Deepa Bhartiya
    Dept of Stem Cell Biology, National Institute for Research in Reproductive Health, JM Street, Parel, Mumbai 400012, India
    J Histochem Cytochem 58:1093-106. 2010
    ..Oct-4A and Oct-4B, in adult human testis. A novel population of 5- to 10-μm PSCs with nuclear Oct-4A was identified by ISH and immunolocalization studies...
  25. ncbi Pluripotency of spermatogonial stem cells from adult mouse testis
    Kaomei Guan
    Department of Cardiology and Pneumology, Heart Center, Georg August University of Gottingen, Robert Koch Str 40, 37075 Gottingen, Germany
    Nature 440:1199-203. 2006
    Embryonic germ cells as well as germline stem cells from neonatal mouse testis are pluripotent and have differentiation potential similar to embryonic stem cells, suggesting that the germline lineage may retain the ability to generate ..
  26. pmc MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis
    Shane T Grivna
    Department of Cell Biology, Duke University Medical School, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:13415-20. 2006
    ..Together, our results indicate that MIWI, a PIWI subfamily protein, uses piRNA as the major, but not exclusive, binding partner, and it is associated with translational machinery...
  27. pmc A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets
    Nikolaus Schultz
    Cecil H and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 100:12201-6. 2003
    ..Here, total mouse testis transcript profiles were trimmed of transcripts found in cultures enriched in Sertoli or interstitial cells to ..
  28. ncbi Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline
    A A Aravin
    Department of Animal Molecular Genetics, Institute of Molecular Genetics, 123182, Moscow, Russia
    Curr Biol 11:1017-27. 2001
    ..The analysis of Caenorhabditis elegans mutants impaired with dsRNA-mediated silencing and studies in plants implicate a biological role of dsRNA-mediated silencing as a transposon-repression and antiviral mechanism...
  29. pmc DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes
    Satomi Kuramochi-Miyagawa
    Department of Pathology, Medical School, Graduate School of Frontier Biosciences, Research Institute for Microbial Diseases, Osaka University, Yamada oka 2 2 Suita, Osaka 565 0871, Japan
    Genes Dev 22:908-17. 2008
    ..Our data strongly suggest that MILI and MIWI2 play essential roles in establishing de novo DNA methylation of retrotransposons in fetal male germ cells...
  30. doi A mouse speciation gene encodes a meiotic histone H3 methyltransferase
    Ondrej Mihola
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
    Science 323:373-5. 2009
    ..These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network...
  31. pmc The germline stem cell niche unit in mammalian testes
    Jon M Oatley
    School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA
    Physiol Rev 92:577-95. 2012
    ..are influenced by a niche microenvironment composed of a growth factor milieu that is provided by several testis somatic support cell populations...
  32. ncbi Expression of a candidate sex-determining gene during mouse testis differentiation
    P Koopman
    Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, Mill Hill, London, UK
    Nature 348:450-2. 1990
    The development of a eutherian mammal as a male is a consequence of testis formation in the embryo, which is thought to be initiated by a gene on the Y chromosome...
  33. ncbi Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over
    S M Baker
    Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201 3098, USA
    Nat Genet 13:336-42. 1996
    ..We also show that Mlh1 appears to localize to sites of crossing over on meiotic chromosomes. Together these findings suggest that Mlh1 is involved in DNA mismatch repair and meiotic crossing over...
  34. doi Identification of the human testis protein phosphatase 1 interactome
    Margarida Fardilha
    Signal Transduction Laboratory, Centre for Cell Biology, Biology Department, University of Aveiro, 3810 193 Aveiro, Portugal
    Biochem Pharmacol 82:1403-15. 2011
    ..The importance of the testis/sperm-enriched variant, PP1γ2, in sperm motility and spermatogenesis has previously been shown...
  35. pmc Vascular-mesenchymal cross-talk through Vegf and Pdgf drives organ patterning
    Jonah Cool
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 108:167-72. 2011
    The initiation of de novo testis cord organization in the fetal gonad is poorly understood. Endothelial cell migration into XY gonads initiates testis morphogenesis...
  36. doi Sox10 gain-of-function causes XX sex reversal in mice: implications for human 22q-linked disorders of sex development
    Juan Carlos Polanco
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Hum Mol Genet 19:506-16. 2010
    ..by the Y-linked gene Sry, which activates expression of Sox9, leading to a cascade of gene activity required for testis formation...
  37. pmc Two distinct origins for Leydig cell progenitors in the fetal testis
    Tony DeFalco
    The Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Dev Biol 352:14-26. 2011
    During the differentiation of the mammalian embryonic testis, two compartments are defined: the testis cords and the interstitium...
  38. ncbi Functional analysis of Sox8 and Sox9 during sex determination in the mouse
    Marie Christine Chaboissier
    INSERM U470, Centre de Biochimie, Parc Valrose, 06108 Nice Cedex 2, France
    Development 131:1891-901. 2004
    Sex determination in mammals directs an initially bipotential gonad to differentiate into either a testis or an ovary...
  39. ncbi Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis
    Dolores D Mruk
    Population Council, Center for Biomedical Research, New York, New York 10021, USA
    Endocr Rev 25:747-806. 2004
    ..generally agreed upon that junction restructuring was involved, at that time the types of junctions present in the testis were not even discerned. Today, it is known that tight, anchoring, and gap junctions are found in the testis...
  40. pmc CTdatabase: a knowledge-base of high-throughput and curated data on cancer-testis antigens
    Luiz Gonzaga Almeida
    Laboratório Nacional de Computação Científica, Petropolis, RJ, Brazil
    Nucleic Acids Res 37:D816-9. 2009
    ..The targets, cancer-testis (CT) antigens, are immunogenic proteins preferentially expressed in normal gametogenic tissues and different ..
  41. ncbi Dax1 antagonizes Sry action in mammalian sex determination
    A Swain
    Division of Developmental Genetics, MRC National Institute for Medical Research, London, UK
    Nature 391:761-7. 1998
    ..XY mice carrying extra copies of mouse Dax1 as a transgene show delayed testis development when the gene is expressed at high levels, but do not normally show sex reversal...
  42. pmc Regulation of spermatogonial stem cell self-renewal in mammals
    Jon M Oatley
    Department of Animal Sciences, Center for Reproductive Biology and Health, College of Agricultural Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Annu Rev Cell Dev Biol 24:263-86. 2008
    ..Together, these molecules are part of a robust gene network controlling SSC fate decisions that may parallel the regulatory networks in other adult stem cell populations...
  43. ncbi The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes
    Helen Skaletsky
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 423:825-37. 2003
    ..9% identity, which is maintained by frequent gene conversion (non-reciprocal transfer). The most prominent features here are eight massive palindromes, at least six of which contain testis genes.
  44. pmc Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage
    Marco Barchi
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell Biol 25:7203-15. 2005
    ..Thus, despite equivalent stages of spermatocyte elimination, different recombination-defective mutants manifest distinct responses, providing insight into surveillance mechanisms in male meiosis...
  45. pmc Endonucleolytic processing of covalent protein-linked DNA double-strand breaks
    Matthew J Neale
    Molecular Biology Programs, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:1053-7. 2005
    ..SPO11-oligonucleotide complexes were identified in extracts of mouse testis, indicating that this mechanism is evolutionarily conserved...
  46. pmc Pluripotent stem cells derived from adult human testes
    Nady Golestaneh
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057, USA
    Stem Cells Dev 18:1115-26. 2009
    ..We obtained testes from organ donors and using 1 g pieces of tissue (biopsy size) we demonstrate that testis germ cells (putative SSCs and/or their progenitors) reprogram to pluripotency when removed from their stem cell ..
  47. pmc MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
    Robert J A Frost
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
    Proc Natl Acad Sci U S A 107:11847-52. 2010
    ..MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility...
  48. pmc Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad
    Kazumichi M Nishida
    Institute for Genome Research, University of Tokushima, Tokushima, Japan
    RNA 13:1911-22. 2007
    ..These results provide the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes...
  49. ncbi A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes
    J Gubbay
    Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, Mill Hill, London, UK
    Nature 346:245-50. 1990
    ..mutant for Tdy, and is expressed at a stage during male gonadal development consistent with its having a role in testis determination...
  50. pmc Spermatogenesis following male germ-cell transplantation
    R L Brinster
    Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104
    Proc Natl Acad Sci U S A 91:11298-302. 1994
    ..This methodology, besides opening new avenues of basic research into spermatogenesis and stem-cell self-renewal, may prove useful as a tool for biomedical science and biotechnology...
  51. ncbi Identification of a testicular odorant receptor mediating human sperm chemotaxis
    Marc Spehr
    Department of Cell Physiology, Ruhr University Bochum, 150 University Street, D 44780 Bochum, Germany
    Science 299:2054-8. 2003
    ..In contrast, undecanal was a potent OR antagonist to bourgeonal and related compounds. Taken together, these results indicate that hOR17-4 functions in human sperm chemotaxis and may be a critical component of the fertilization process...
  52. ncbi Expression of Mina53, a product of a Myc target gene in mouse testis
    Makoto Tsuneoka
    Division of Human Genetics, Department of Forensic Medicine, Kurume University School of Medicine, Kurume, Japan
    Int J Androl 29:323-30. 2006
    ..Here we report the expression of Mina53 in mouse testis, which contains proliferating cells and expresses many cancer-related genes...
  53. pmc MicroRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 3:e1738. 2008
    ..Although it has been clear that piRNAs play a role in repression of retrotransposons during spermatogenesis, the function of miRNA in mouse germ cells has been unclear...
  54. doi Effect of mono-(2-ethylhexyl) phthalate on human and mouse fetal testis: In vitro and in vivo approaches
    V Muczynski
    Unit of Stem Cells and Radiation, Laboratory of Development of the Gonads, Univ Paris Diderot, Sorbonne Paris Cité, BP 6, 92265 Fontenay aux Roses, France
    Toxicol Appl Pharmacol 261:97-104. 2012
    ..This study suggests that this 10⁻⁵ M exposure is sufficient to induce changes to the in vivo development of the human fetal male germ cells...
  55. pmc Gadd45γ and Map3k4 interactions regulate mouse testis determination via p38 MAPK-mediated control of Sry expression
    Nick Warr
    Mammalian Genetics Unit, Medical Research Council, Harwell, Oxfordshire OX11 0RD, UK
    Dev Cell 23:1020-31. 2012
    Loss of the kinase MAP3K4 causes mouse embryonic gonadal sex reversal due to reduced expression of the testis-determining gene, Sry...
  56. doi Paternal obesity initiates metabolic disturbances in two generations of mice with incomplete penetrance to the F2 generation and alters the transcriptional profile of testis and sperm microRNA content
    Tod Fullston
    1Level 3 Medical School South, University of Adelaide, Adelaide, SA, Australia 5005
    FASEB J 27:4226-43. 2013
    ..b>Testis expression of mRNA/microRNAs was analyzed by microarray and qPCR and sperm microRNA abundance by qPCR...
  57. ncbi miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis
    Wei Deng
    Department of Cell Biology, Duke University Medical Center, P O Box 3709, DUMC, Durham, NC 27710, USA
    Dev Cell 2:819-30. 2002
    ..Furthermore, mRNAs of ACT (activator of CREM in testis) and CREM target genes are downregulated in miwi(null) testes...
  58. ncbi Cancer/testis antigens: an expanding family of targets for cancer immunotherapy
    Matthew J Scanlan
    Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan Kettering Cancer Center, Weill Medical College of Cornell University, Department of Pathology, New York, NY 10021, USA
    Immunol Rev 188:22-32. 2002
    Cancer/testis (CT) antigens are a category of tumor antigens with normal expression restricted to male germ cells in the testis but not in adult somatic tissues. In some cases, CT antigens are also expressed in ovary and in trophoblast...
  59. pmc Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1beta and SMC3
    Maureen Eijpe
    Molecular Genetics Group, Botanical Center, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, Netherlands
    J Cell Biol 160:657-70. 2003
    ..We propose that REC8 then provides cohesion. RAD51 and/or DMC1 coimmunoprecipitates with REC8, suggesting that REC8 may also provide a basis for assembly of recombination complexes...
  60. ncbi Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
    Henri Bastos
    Laboratoire de Gametogenèse, Apoptose et Génotoxicité, DRR DSV CEA, U566 INSERM, Universite Paris 7, Fontenay aux Roses, France
    Cytometry A 65:40-9. 2005
    ..The cellular heterogeneity of testis renders complex the studies on molecular aspects of this differentiation process...
  61. ncbi Glial cell-line derived neurotrophic factor-mediated RET signaling regulates spermatogonial stem cell fate
    Cathy K Naughton
    Division of Urology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Biol Reprod 74:314-21. 2006
    ..We used the whole-testis transplantation technique to overcome the limitation of neonatal lethality of Gdnf-, Gfra1-, and Ret-deficient ..
  62. ncbi Cancer-testis genes are coordinately expressed and are markers of poor outcome in non-small cell lung cancer
    Ali O Gure
    Ludwig Institute for Cancer Research, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Clin Cancer Res 11:8055-62. 2005
    Cancer-testis genes mapping to the X chromosome have common expression patterns and show similar responses to modulators of epigenetic mechanisms...
  63. pmc Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro
    Hiroaki Tachiwana
    Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nucleic Acids Res 36:2208-18. 2008
    ..1, H3.2, H3.3, H3t and CENP-A, have been identified in mammals. H3t is robustly expressed in the testis, and thus was assigned as the testis-specific H3 variant...
  64. doi The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells
    Aiko Sada
    Department of Genetics, SOKENDAI, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Science 325:1394-8. 2009
    ..Thus, NANOS2 is a key stem cell regulator that is expressed in self-renewing spermatogonial stem cells and maintains the stem cell state during murine spermatogenesis...
  65. doi FGF9 suppresses meiosis and promotes male germ cell fate in mice
    Josephine Bowles
    Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Dev Cell 19:440-9. 2010
    ..acid (RA) triggers ovarian germ cells to enter meiosis and thereby commit to oogenesis, whereas in the developing testis, the enzyme CYP26B1 degrades RA and germ cells are not induced to enter meiosis...
  66. pmc MicroRNA and piRNA profiles in normal human testis detected by next generation sequencing
    Qingling Yang
    Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China
    PLoS ONE 8:e66809. 2013
    ..However, there are no published studies of miRNA profiles in human testis using next generation sequencing (NGS) technology.
  67. pmc Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells
    Nina Kossack
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Palo Alto, California 94304 5542, USA
    Stem Cells 27:138-49. 2009
    ..pluripotent cells (multipotent germline stem cells) from spermatogonial stem cells obtained from the adult mouse testis. These spermatogonia-derived stem cells express embryonic stem cell markers and differentiate to the three primary ..
  68. doi Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka
    Yasushi Shibata
    Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Gene Expr Patterns 10:283-9. 2010
    ..When the XY embryos were treated with estradiol-17beta, in order to reverse their phenotypic sex, a decline was observed in the expression of Gsdf in these embryos by real-time PCR...
  69. doi Hypermethylation of estrogen receptor promoter region in adult testis of rats exposed neonatally to bisphenol A
    Tanvi Doshi
    National Center for Preclinical Reproductive and Genetic Toxicology, National Institute for Research in Reproductive Health ICMR, J M Street, Parel, Mumbai 400012, Maharashtra, India
    Toxicology 289:74-82. 2011
    ..Further we also observed an altered gene expression of ERα and ERβ in adult testis upon BPA exposure...
  70. pmc Role of testis-specific gene expression in sex-chromosome evolution of Anopheles gambiae
    Dean A Baker
    Department of Genetics, University of Cambridge, Cambridge CB1 3QA, United Kingdom
    Genetics 189:1117-20. 2011
    Gene expression in Anopheles gambiae shows a deficiency of testis-expressed genes on the X chromosome associated with an excessive movement of retrogene duplication...
  71. doi Testicular dysgenesis syndrome: mechanistic insights and potential new downstream effects
    Richard M Sharpe
    MRC Human Reproductive Sciences Unit, Centre for Reproductive Biology, The Queen s Medical Research Institute, Edinburgh, United Kingdom
    Fertil Steril 89:e33-8. 2008
    ..in understanding from such studies have led to refinement of the TDS hypothesis, highlighting the central role that deficient androgen production/action during fetal testis development, may play in the origin of downstream disorders.
  72. pmc Stabilization of beta-catenin in XY gonads causes male-to-female sex-reversal
    Danielle M Maatouk
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Hum Mol Genet 17:2949-55. 2008
    ..is opposed by the expression of two signals, WNT4 and R-SPONDIN-1 (RSPO1), that promote the ovarian fate and block testis development. Both of these ligands can activate the canonical Wnt signaling pathway...
  73. ncbi The Drosophila ovarian and testis stem cell niches: similar somatic stem cells and signals
    Eva Decotto
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA
    Dev Cell 9:501-10. 2005
    ..While both contain germline stem cells, the testis niche also contains "cyst progenitor" stem cells, which divide to produce somatic cells that encase developing ..
  74. doi Sex determination and SRY: down to a wink and a nudge?
    Ryohei Sekido
    Division of Developmental Genetics, Medical Research Council, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Trends Genet 25:19-29. 2009
    ..review, we focus on the regulation of these genes and their interaction with other genes involved in promoting testis or ovary development...
  75. pmc Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis
    Jun Yan Jin
    State Key Laboratory of Freshwater Ecology and Biotechnology, Wuhan Center for Developmental Biology, Institute of Hydrobiology, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Wuhan, China
    PLoS ONE 5:e12883. 2010
    ..Interestingly, the β-defensin was shown to be dominantly expressed in pituitary and testis by RT-PCR and Western blot analysis, and its transcript level is significantly upregulated in reproduction organs ..
  76. ncbi Gene expression in rat leydig cells during development from the progenitor to adult stage: a cluster analysis
    Ren Shan Ge
    The Population Council, New York, New York 10021, USA
    Biol Reprod 72:1405-15. 2005
    ..three distinct stages: initially they exist as fibroblast-like progenitor Leydig cells (PLCs) appearing in the testis by Days 14-21; subsequently, by Day 35, they become immature Leydig cells (ILCs) acquiring steroidogenic organelle ..
  77. pmc The DNA sequence of the human X chromosome
    Mark T Ross
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
    Nature 434:325-37. 2005
    ..We found 1,098 genes in the sequence, of which 99 encode proteins expressed in testis and in various tumour types...
  78. pmc Generation of functional multipotent adult stem cells from GPR125+ germline progenitors
    Marco Seandel
    Howard Hughes Medical Institute, Department of Genetic Medicine, Weill Cornell Medical College, New York 10065, USA
    Nature 449:346-50. 2007
    Adult mammalian testis is a source of pluripotent stem cells. However, the lack of specific surface markers has hampered identification and tracking of the unrecognized subset of germ cells that gives rise to multipotent cells...
  79. ncbi Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility
    T R Kumar
    Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 15:201-4. 1997
    ..Our findings have important implications for male contraceptive development in humans...
  80. ncbi Soluble adenylyl cyclase as an evolutionarily conserved bicarbonate sensor
    Y Chen
    Department of Pharmacology, Joan and Sanford I Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Science 289:625-8. 2000
    ..sAC is also expressed in other bicarbonate-responsive tissues, which suggests that bicarbonate regulation of cAMP signaling plays a fundamental role in many biological systems...
  81. ncbi Primate spermatogonial stem cells colonize mouse testes
    M Nagano
    Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6009, USA
    Biol Reprod 64:1409-16. 2001
    ..Attempts to extend this approach by transplanting human testis cells to mice have led to conflicting claims that no donor germ cells persisted or that human spermatozoa were ..
  82. ncbi A transgenic insertion causing cryptorchidism in mice
    P A Overbeek
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
    Genesis 30:26-35. 2001
    ..A candidate gene encoding a novel G protein-coupled receptor (Great) with an expression pattern suggesting involvement in testicular descent has been identified...
  83. ncbi Androgen receptor function is required in Sertoli cells for the terminal differentiation of haploid spermatids
    Robert W Holdcraft
    University of Washington School of Medicine, Department of Genome Sciences, Seattle, WA 98195, USA
    Development 131:459-67. 2004
    ..their release from the seminiferous epithelium is AR dependent and maximally sensitive to AR depletion within the testis. Cell-specific disruption of Ar in Sertoli cells of hypomorphic animals further shows that progression of late-..
  84. pmc Identification of mouse SLC39A8 as the transporter responsible for cadmium-induced toxicity in the testis
    Timothy P Dalton
    Department of Environmental Health and the Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, OH 45267 0056, USA
    Proc Natl Acad Sci U S A 102:3401-6. 2005
    ..mice; by using situ hybridization, we found that ZIP8 mRNA is prominent in the vascular endothelial cells of the testis of the sensitive strains of mice but absent in these cells of resistant strains...
  85. doi Presumed pluripotency markers UTF-1 and REX-1 are expressed in human adult testes and germ cell neoplasms
    David M Kristensen
    University Department of Growth and Reproduction, Section GR5064, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark
    Hum Reprod 23:775-82. 2008
    ....
  86. pmc Profiling gene expression during the differentiation and development of the murine embryonic gonad
    Christopher L Small
    Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
    Biol Reprod 72:492-501. 2005
    ..As expected, the expression of two well-known sex determination genes, specifically Sry and Sox9, is unique to the testis. Beyond 12...
  87. ncbi Generation of pluripotent stem cells from neonatal mouse testis
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell 119:1001-12. 2004
    ..Here we report the successful establishment of ES-like cells from neonatal mouse testis. These ES-like cells were phenotypically similar to ES/EG cells except in their genomic imprinting pattern...
  88. pmc Characterization of E3Histone, a novel testis ubiquitin protein ligase which ubiquitinates histones
    Zhiqian Liu
    Polypeptide Hormone Laboratory, McGill University, Strathcona Anatomy and Dentistry Building, 3640 University St, Room W315, Montreal, Quebec, Canada, H3A 2B2
    Mol Cell Biol 25:2819-31. 2005
    ..This activation is dependent on the ubiquitin-conjugating enzyme (E2) UBC4, and a testis-particular isoform, UBC4-testis, is induced when histones are degraded...
  89. ncbi Male fertility in natural populations of red deer is determined by sperm velocity and the proportion of normal spermatozoa
    Aurelio F Malo
    Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales CSIC, 28006 Madrid, Spain
    Biol Reprod 72:822-9. 2005
    ..We conclude that male fertility varies substantially in natural populations of Iberian red deer and that, when sperm numbers are equal, it is determined mainly by sperm swimming velocity and sperm morphology...
  90. ncbi Functional coherence of the human Y chromosome
    B T Lahn
    Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 278:675-80. 1997
    ..The coherence of the NRY's gene content contrasts with the apparently haphazard content of most eukaryotic chromosomes...
  91. pmc Male germ cells express abundant endogenous siRNAs
    Rui Song
    Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA
    Proc Natl Acad Sci U S A 108:13159-64. 2011
    ..Overall, our work has unveiled another hidden layer of regulation imposed by small noncoding RNAs during male germ cell development...
  92. ncbi Conserved role of nanos proteins in germ cell development
    Masayuki Tsuda
    Division of Mammalian Development, National Institute of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Science 301:1239-41. 2003
    ..Hence, although mice and flies differ in their mechanisms for germ cell specification, there seems to be conserved function for nanos proteins among invertebrates and vertebrates...
  93. ncbi Nanog expression in mouse germ cell development
    Shinpei Yamaguchi
    Laboratory of Stem Cell Engineering, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo ku, Japan
    Gene Expr Patterns 5:639-46. 2005
    ..No germ cells positive for NANOG were detectable in testes and ovaries of adult mice. Thus, in germ cell development, NANOG is expressed in proliferating germ cells, in which nuclear reprogramming is progressing...
  94. ncbi Histological evaluation of the human testis--approaches to optimizing the clinical value of the assessment: mini review
    R I McLachlan
    Prince Henry s Institute, Monash University, Clayton, Victoria, Australia
    Hum Reprod 22:2-16. 2007
    ..The rising incidence of testis cancer and carcinoma in situ (CIS), especially in infertile populations, requires that every effort be made for ..
  95. pmc Foxl2 functions in sex determination and histogenesis throughout mouse ovary development
    Jose Elias Garcia-Ortiz
    Laboratory of Genetics, NIA NIH IRP, Baltimore, MD, USA
    BMC Dev Biol 9:36. 2009
    ..g., Rspo1. Here we report expression profiling of mouse ovaries that lack Foxl2 alone or in combination with Wnt4 or Kit/c-Kit...
  96. pmc Mutations in NR5A1 associated with ovarian insufficiency
    Diana Lourenco
    Human Developmental Genetics, Institut Pasteur, Paris, France
    N Engl J Med 360:1200-10. 2009
    ..Mutation of NR5A1 causes 46,XY disorders of sex development, with or without adrenal failure, but growing experimental evidence from studies in mice suggests a key role for this factor in ovarian development and function as well...
  97. ncbi A mutation in the gene encoding steroidogenic factor-1 causes XY sex reversal and adrenal failure in humans
    J C Achermann
    Nat Genet 22:125-6. 1999
  98. ncbi Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis
    Guenter Haemmerle
    Institute of Molecular Biology, Biochemistry, and Microbiology, University of Graz, Graz A 8010, Austria
    J Biol Chem 277:4806-15. 2002
    ..causes the accumulation of DG in white adipose tissue, brown adipose tissue, skeletal muscle, cardiac muscle, and testis. Second, when tissue extracts were used in an in vitro lipase assay, a reduced FFA release and the accumulation of ..
  99. ncbi Male-to-female sex reversal in mice lacking fibroblast growth factor 9
    J S Colvin
    Department of Molecular Biology and, Pharmacology, Washington University Medical School, Campus Box 8103, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Cell 104:875-89. 2001
    ..stimulate mesenchymal proliferation, mesonephric cell migration, and Sertoli cell differentiation in the embryonic testis. While Sry is found only in some mammals, Fgfs are highly conserved...
  100. ncbi MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline
    Michelle A Carmell
    Cold Spring Harbor Laboratory, Howard Hughes Medical Institute, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Dev Cell 12:503-14. 2007
    ..These phenotypes may be linked to an inappropriate activation of transposable elements detected in Miwi2 mutants. Our observations suggest a conserved function for Piwi-clade proteins in the control of transposons in the germline...
  101. pmc Generation of Cyp17iCre transgenic mice and their application to conditionally delete estrogen receptor alpha (Esr1) from the ovary and testis
    Phillip J Bridges
    Division of Clinical and Reproductive Sciences, Department of Clinical Sciences, University of Kentucky, Kentucky 40536, USA
    Genesis 46:499-505. 2008
    ..conditional deletion of genes specifically in the theca/interstitial cells of the ovary and Leydig cells of the testis. In this report, we describe the development of Cyp17iCre mice and the application of these mice for conditional ..

Research Grants101 found, 100 shown here

  1. Xenoestrogen regulation of Leydig cells
    BENSON TOKUNBO AKINGBEMI; Fiscal Year: 2010
    ..Also, soy isoflavones are known to regulate development of somatic cells in the testis, e.g. Sertoli cells. Therefore, cell-cell interactions, e.g...
  2. Yun Fai Chris Lau; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): The testis-specific protein Y-encoded (TSPY) gene is a tandemly repeated gene on the short arm of the human Y chromosome. Its 2...
  3. Ming Zhou; Fiscal Year: 2014
    ..mammals, urea transporters are expressed in a wide array of organs, such as kidney, brain, heart, liver, ear, and testis, suggesting that they play an important role in physiology...
  4. John H Postlethwait; Fiscal Year: 2016
    ..mammals, the Y-chromosome gene SRY initiates an incompletely understood network that directs the gonad to become a testis rather than an ovary...
  5. Interdisciplinary Research Training in Reproduction for Indian Fellows
    John C Herr; Fiscal Year: 2010
    ..of this research include the development of male contraceptives targeted to transcription factors acting in the testis and epididymis, spermicides, infertility diagnostics, and contraceptive vaccines based on sperm and egg antigens...
  6. Regulation of insulins by the Rhox5 homeobox gene supports spermatogenesis
    James Maclean; Fiscal Year: 2009
    ..Thus, the characterization of Rhox5-null mice may also be useful as a model system for understanding human fertility problems. ..
  7. Lois B Travis; Fiscal Year: 2016
    ..rectum, cervix, endometrium, bladder, stomach, head and neck, lung, esophagus, pancreas, osteosarcoma, ovary, and testis, for which first-line therapy can potentially include platinating agents...
  8. Ameae M Walker; Fiscal Year: 2015
    ..core genotype mouse model, to determine whether the sex effect is caused by sex chromosome complement or by the testis-determining gene, Sry...
  9. Sienmi Du; Fiscal Year: 2014
    ....
  10. Erythropoietin in Non-Erythroid Cells: Function and Regulation
    Josef T Prchal; Fiscal Year: 2013
    ..The possibility that Epo may play a beneficial as well as detrimental role in endothelial/cardiovascular, neurological abnormalities, and cancers is relevant to a significant portion of VA patients. ..
  11. Tracie R Baker; Fiscal Year: 2016
    ..The University of Wisconsin has numerous NIH-sponsored training programs, core facilities and funded researchers making it an ideal training environment for me to develop as an independent scientist. ..
  12. THE EFFECT OF DIOXINS ON GLUCOSE HOMEOSTASIS IN MURINE TESTES
    KENAN RIFAT OMURTAG; Fiscal Year: 2013
    ..Moreover we have established a Laser Microdissection protocol for testis sections and have successful validate our techniques...
  13. The Mechanism of Cryptorchidism in LH Receptor Knockout Animals
    Zhenmin Lei; Fiscal Year: 2012
    ..3 (INSL3) and androgen signaling pathways play critical roles in the control of the transabdominal phase of testis descent...
  14. C Yan Cheng; Fiscal Year: 2016
    The long-term objective of this proposal is to understand the biology and regulation of blood-testis barrier (BTB) dynamics during spermatogenesis using the adult rat testis as a study model...
  15. StAR expression: Integration of Transcription with regulation via the mRNA 3'UTR
    COLIN ROBERT JEFCOATE; Fiscal Year: 2012
    ..TIS11b regulation is present in other tissues that make steroids (testis)...
  16. Gender Bias in Lupus: Contribution of Sex Chromosomes
    Ram Raj Singh; Fiscal Year: 2012
    ..The results of the proposed study will not only aid our understanding of lupus, but will also potentially lead to identification of newer targets of treatment. ..
  17. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2012
    ..germ cells in humans is thought to be the cause of germinomas, tumors of the germ line that develop outside of the testis and ovaries...
  18. Model of the Human Testis for Reproductive Toxicology
    PAUL JACOB TUREK; Fiscal Year: 2011
    ..The overall goal of the research is to develop a functional in vitro human cell-based testis model of spermatogenesis as a clinically relevant product for reproductive toxicology...
  19. Regulation of Rhox5 Transcriptional Elongation
    Marcy E Richardson; Fiscal Year: 2013
    ..The control of these two phenomena will be analyzed in the developmental and tissue-specific program that allows the expression of Rhox5 Pp in the mouse testis.
  20. Clinton C MacDonald; Fiscal Year: 2015
    ..DCstF-64 (gene name: Cstf2t) is the testis-expressed variant of CstF-64, an RNA-binding protein that regulates polyadenylation in somatic cells...
  21. Integrative Pathway Analysis of Epigenomic/transcriptional Alteration in HNSCC
    Joseph A Califano; Fiscal Year: 2010
    ....
  22. AMANDA MARIE LARRACUENTE; Fiscal Year: 2014
    ..and second, to describe the distribution of Rsp repeat-associated short interfering RNAs (rasiRNAs) in the testis and test if they are disrupted in SD/SD+ heterozygotes...
  23. Russ B Hauser; Fiscal Year: 2015
    ..pubertal development (testosterone, inhibin- B, MIS, LH, FSH, IGF-1, IGF-BP3, TSH and free T4);4) altered adult testis function, specifically semen quality and hormonal markers of mature testis function and the reproductive axis (..
  24. Drug Transport at the Blood-Testis Barrier
    Nathan J Cherrington; Fiscal Year: 2013
    ..and possible development of a resistant phenotype: the blood- brain barrier (BBB) and the blood-testis barrier (BTB)...
  25. Molecular Analysis of GnRH Action
    Mark S Roberson; Fiscal Year: 2012
    ..Pituitary-derived gonadotropic hormones in turn regulate the function of the ovary and testis. Manipulation of this system using specific drugs to affect GnRH action can have a large impact on fertility...
  26. Ruth Lehmann; Fiscal Year: 2016
    ..germ cells in humans is thought to be the cause of germinomas, tumors of the germ line that develop outside of the testis and ovaries...
  27. Functions of the Human OST-alpha and OST-beta Proteins
    Patricia M Hinkle; Fiscal Year: 2013
    ..transporter that is expressed in nearly all tissues, but is most abundant in the small intestine, kidney, liver, testis, adrenal gland, as well as other steroidogenic tissues...
  28. Guo Zhang Zhu; Fiscal Year: 2014
    ..Furthermore, collected information may offer insights into unexplained (or idiopathic) infertility in men and may lead to new strategies for male contraception and assisted reproduction. ..
  29. Regulation and Function of the Rhox8 homeobox gene in granulosa cells
    JAMES ARTHUR MACLEAN; Fiscal Year: 2010
    ..While expression of the Rhox genes is restricted to reproductive tissues, ovary, testis, epididymis, and placenta, very few have been examined for function...
  30. Meiosis, SUMOylation and the ZIP3 Protein: Parallel Studies in Mouse and Yeast.
    Neil Hunter; Fiscal Year: 2012
    ..We will also utilize yeast 2-hybrid screening and co-immunoprecipitation from testis extracts to identify MmZIP3 partners and substrates...
  31. WNT and gonadotropin mediated regulation of ovarian steroidogenesis
    JENNIFER HERNANDEZ GIFFORD; Fiscal Year: 2010
    ....
  32. Targeting Glucose Metabolism in Cancer
    Sucheta Telang; Fiscal Year: 2013
    ..reportedly expressed in distinct tissues: liver/skeletal muscle (PFKFB1), heart (PFKFB2), placenta (PFKFB3) and testis (PFKFB4)...
  33. Listeria-Based Therapeutic Cancer Vaccine for Melanoma
    Dirk G Brockstedt; Fiscal Year: 2011
    ..select a clinical vaccine candidate strain based on photochemically-inactivated KBMA Lm that express the cancer-testis antigens NY-ESO-1 and MAGE-A3 as a therapeutic melanoma vaccine and ultimately evaluate the safety and efficacy in ..
  34. Catenins: A role in spertmatogenesis and sperm maturation
    MANJEET KUMAR RAO; Fiscal Year: 2010
    ..junctional complexes formed between Sertoli cells (basal ES) and between Sertoli and germ cells (apical ES) in the testis. The importance of this junctional complex is evidenced by the fact that the abnormal or disrupted ES contributes ..
  35. Role of nuclear receptors in origin and differentiation of spermatozoa
    ADAM MICHAEL REITZEL; Fiscal Year: 2011
    ....
  36. Eric Gustafson; Fiscal Year: 2015
    ..the role of TAF4b in the establishment and maintenance of the self-renewing lineage of SSCs in the mouse testis. TAF4b is a gonad-enriched general transcription factor varient and both male and female Taf4b-deficient mice ..
  37. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2011
    ..The human proteins, testis-specific protein, Y-encoded-like (TSPYL1) and fetal globin inducing factor (FGIF or ANKRD49), have been shown to ..
  38. Paul F Pilch; Fiscal Year: 2015
    ..By understanding the mechanism of Arx action, these consequences can be addressed by appropriate therapeutic strategies. ..
  39. Ina Dobrinski; Fiscal Year: 2016
    ..The testis stem cell is unique in that it is the only cell type in an adult individual that divides and contributes genes to ..
  40. Towards a Model of Fanconi Anemia
    BEVERLY JO TOROK STORB; Fiscal Year: 2010
    ..SSC are the cells residing in the testis that continue to generate sperm for the life of the animal...
  41. The XXII nd North American Testis Workshop
    WILLIAM WALLACE WRIGHT; Fiscal Year: 2013
    ..provided by applicant): This R13 conference grant application requests funds to support the XXIInd North American Testis Workshop that will be held from April 13 to April 16, 2013 in San Antonio, Texas...
  42. Biology of desmosome-gap junctions in the testis
    Dolores D Mruk; Fiscal Year: 2010
    ..In the testis, morphological studies in the 1970s reported the presence of desmosome-gap junctions (also known as desmosome-like ..
  43. Structural Targeting Testis Specific Kinases (TSSK) 1 &2 for Male Contraception
    John C Herr; Fiscal Year: 2013
    The testis specific serine threonine kinases 1 &2 [TSSK 1 &2] are closely related genes that have recently been validated as male contraceptive targets...
  44. The MRAD9 Radioresistance Gene
    Howard B Lieberman; Fiscal Year: 2013
    ..role in spermatogenesis and in the meiotic cell cycle by regulating genomic stability, apoptosis and repair in testis. These studies will examine Mrad9 function from molecular to cellular to whole animal levels...
  45. MOLECULAR BASIS OF MAMMALIAN SPERM MOTILITY
    Jerome F Strauss; Fiscal Year: 2010
    ..proteins, the 71 kDa PF20I, localized to the axoneme central apparatus, and a second protein transcribed from a testis-specific promoter, 35 kDa PF20s, which contains the C-terminus of PF20I, but is localized to the germ cell nucleus...
  46. Debra J Wolgemuth; Fiscal Year: 2016
    ..We showed that cyclin A1 is testis-specific and by targeted mutagenesis, that it is essential for spermatogenesis--spermatocytes arrest at the ..
  47. Margaret T Fuller; Fiscal Year: 2016
    ..We discovered that testis-specific TAFs, homologs of components of the general PolII transcription machinery, turn on expression of ..
  48. MOUSE MODELS TO STUDY GONADAL TUMOR DEVELOPMENT
    Martin M Matzuk; Fiscal Year: 2010
    ..Characterization of these mice will lead to important insights into the development of gonadal cancers in men and women and the roles of several proteins in reproductive physiology. ..
  49. BDADs for Male Contraception
    John K Amory; Fiscal Year: 2013
    ..The research described in this proposal may eventually allow for the development of a safe and effective oral approach to male contraception, which will serve to greatly decrease the risk of unintended pregnancy and population growth. ..
  50. ESTHER BETRAN; Fiscal Year: 2014
    ..e., selection operating already on the parental gene) driven by tissue antagonism (i.e., testis antagonism)...
  51. Regulation of Germ Cell Pluripotency Through The RNA-Binding Protein, DND1
    Blanche Capel; Fiscal Year: 2013
    ..specification/migration stages, (2) during the fetal stage of germ cell transition to pro- spermatogonia in the testis and (3) in postnatal animals...
  52. Wei Yan; Fiscal Year: 2014
    DESCRIPTION (provided by applicant): Leydig cells are interstitial cells of the testis and represent the major source of testosterone (T) production in men...
  53. PAMELA M STANLEY; Fiscal Year: 2016
    ..Importantly, the GnT1IP gene is robustly expressed in testis, and reduced expression correlates with infertility syndromes in humans...
  54. Monoallelic repair of expanded huntingtin by trans-splicing
    Hansjorg Rindt; Fiscal Year: 2011
    ..An inherited mutation causes the death of neurons in the brain. This project explores the possibility of repairing the mutation and to restore normal cellular functions. ..
  55. The epigenetic basis of cytoplasmic incompatibility
    KRISTIN KALIE JERNIGAN; Fiscal Year: 2012
    ..This research will determine, for the first time, if Wolbachia induces an infectious epigenetic alteration to cause CI. ..
  56. The molecular basis for a broad range of phenotypes related to hTAS2R38
    Sarah V Lipchock; Fiscal Year: 2012
    ..in the proposed studies will be useful for understanding other aspects of the taste system, including the other four basic taste qualities and expression of taste receptors in nontaste tissue such as pancreas and testis.
  57. Cyclic AMP Action During Sperm Function
    George L Gerton; Fiscal Year: 2012
    ..Although spermatogenesis in the testis appears to proceed undisturbed, the morphologically normal sperm are immotile...
  58. Epigenetic Regulation in Self-Renewing and Differentiating Male Germ Cells
    CHRISTOPHER JESS PAYNE; Fiscal Year: 2012
    ..This project is now poised to enter the ROO phase, extending the progress made during the K99 phase and achieving the major aspects of both specific aims. ..
  59. Characterization of male germline stem cells in a rhesus model of infertility
    Kyle E Orwig; Fiscal Year: 2012
    ..treatment regimen that causes long- term infertility in rhesus macaques to facilitate autologous and homologous testis cell transplantation...
  60. STUDIES ON OVARIAN RING CANALS IN DROSOPHILA
    Lynn Cooley; Fiscal Year: 2009
    ..A phenotype associated with loss of follicle cells ring canals will likely provide the first insight into their function in epithelial cells. ..
  61. Yingming Zhao; Fiscal Year: 2016
    ..following meiosis, histone Kcr, but not histone Kac, is enriched on sex chromosomes and specifically marks testis-specific genes...
  62. 3-Dimensional modeling of basal cell function in pseudostratified epithelia
    Sylvie Breton; Fiscal Year: 2013
    ..The epididymis, which connects the testis to the vas deferens, is involved in the maturation and storage of spermatozoa and, therefore, plays a crucial role ..
  63. Vicki N Meyers-Wallen; Fiscal Year: 2014
    ..In the testis pathway, SOX9 expression is critical to testis induction...
  64. AUDE CHAPUIS; Fiscal Year: 2016
    ..She then proposes to explore the feasibility of targeting the newly identified LSC and cancer testis antigen CCNA1 with T cell therapy by testing if non-gene modified T-cell products from leukemic donors can be ..
  65. Peter Sicinski; Fiscal Year: 2016
    ..Specific Aim 2. To determine the molecular function of cyclin A-Cdk1 in stem cells. Specific Aim 3. To test the requirement for cyclin A function in Myc-driven breast cancers. ..
  66. Monika A Ward; Fiscal Year: 2016
    ..We have established that only two Y chromosome genes, testis determinant gene Sry and spermatogonial proliferation factor Eif2s3y are required for production of male gametes ..
  67. Aaron P Rapoport; Fiscal Year: 2014
    ..These cancer/testis tumor antigens (CTAgs) are frequently and specifically expressed in myeloma cells...
  68. Barry R Zirkin; Fiscal Year: 2015
    ..balance using fixed tissue. ..
  69. STEVEN CARMEN MUNGER; Fiscal Year: 2014
    ..The gonad arises at mid-gestation competent to differentiate as a testis or ovary irrespective of sex chromosome constitution...
  70. DANA LEANNE JONES; Fiscal Year: 2016
    ..In the Drosophila testis, stem cells surround and contact the apical hub, a cluster of somatic cells that express the self-renewal factor ..
  71. Xiaozhong Yu; Fiscal Year: 2014
    A 3D mini-testis model for reproductive toxicity testing Project Summary Occupational exposure to reproductive toxicants occurs via inhalation, skin absorption, or ingestion...
  72. Eludicating the Molecular Mechanisms That Regulate Stem Cell Numbers in Vivo
    Erika A Bach; Fiscal Year: 2013
    ..system to address this issue, as its control of stem cell numbers is conserved in several tissues, including the testis and eye. Drosophila has only one jak and one stat (called stat92E) and offers facile in vivo analysis...
  73. RENEE GEORGE; Fiscal Year: 2015
    ..First, a large fraction of genes on these chromosomes are expressed in the testis and thus are likely to function in male specific processes like spermatogenesis...
  74. Kimberly A Paczolt; Fiscal Year: 2015
    ....
  75. Genetic and cellular sex differences in cerebellum
    EMILLIE F RISSMAN; Fiscal Year: 2013
    ..This program offers a novel perspective on sex differences in cerebellar function based on sex chromosome genes;this will lead to innovations in diagnostics and interventions for patients with neurological motor diseases. ..
  76. Helen E Heslop; Fiscal Year: 2016
    ..Rooney and Bollard will validate new target antigens in Hodgkin's lymphoma, particularly cancer testis antigens, before testing cytotoxic T lymphocytes (CTLs) specific for these targets in patients with relapsed ..
  77. Kodi S Ravichandran; Fiscal Year: 2016
    ..Importantly, data from human testis biopsies collected after testicular torsion will be correlated with data from the mouse model...
  78. Kodi S Ravichandran; Fiscal Year: 2016
    ..Since altered expression of ELMO1 and BAI1 are genetically linked to inflammatory disorders in humans, the results from the proposed studies could be relevant for therapeutic intervention in inflammatory disorders. ..
  79. Clonal competition in stem cells as a driver of paternal age effect diseases
    Marco Seandel; Fiscal Year: 2013
    ..g., autism and schizophrenia). Positive selection of mutant spermatogonial stem cells (SSCs) in the human testis may enable aberrant sperm to contribute such de novo mutant alleles to the next generation, resulting in the ..
  80. Elucidating the effectors of cell clearance in immunoprivileged organs
    Jon Iker Etchegaray; Fiscal Year: 2013
    ..These organs are termed immunoprivileged. Immunoprivileged organs include the eyes, the testis and the brain, among others...
  81. Philip W Jordan; Fiscal Year: 2015
    ..I am creating a germ cell-specific mutation of Smc5 and mutating the testis specific SMC5/6 gene Eid3...
  82. The maintenance of Drosophila testis stem cells during starvation
    HEIKO HUI YANG; Fiscal Year: 2013
    ..gain insight into the biological basis of this phenomenon, we explored the effects of starvation in the Drosophila testis, a model system in which GSCs can be clearly identified and distinguished from their spermatogonia (SG) daughters...
  83. Prenatal Bisphenol A and Lung Maturation
    Laura S Van Winkle; Fiscal Year: 2013
    ..early pre or neonatal exposure to low dose of BPA resulting in organizational changes in the prostate, breast, testis, mammary glands of laboratory animals...
  84. The Sertoli Cell Product, Doppel, and Male Fertility
    William Wright; Fiscal Year: 2009
    ..Second, the testis expresses substantially higher concentrations of Prnd mRNA than any other organ...
  85. Cisplatin and Mechanisms of Long-term Male Infertility
    JOHN RICHBURG; Fiscal Year: 2007
    ..This indicates damage to other somatic cells within the testis. Histopathology of testes from cisplatin-treated mice revealed large Sertoli cell vacuoles, apical sloughing, ..
  86. INCREASED TESTICULAR CD2+ & INFERTILITY WITH VARICOCELE
    Susan Benoff; Fiscal Year: 2004
    ..Controls will include fertile donors and fertile men with varicocele. ..
  87. Effect of chronic low-dose phthalate exposure on the immature primate testis
    Ina Dobrinski; Fiscal Year: 2009
    ..exploratory research project is to study the effects of chronic exposure to phthalate esters on the infant primate testis in a novel xenograft model system...
  88. INHIBIN GENE EXPRESSION IN TESTIS AND SERTOLI CELLS
    HUGH KEEPING; Fiscal Year: 1991
    Inhibin, a glycoprotein synthesized by the Sertoli cells of the seminiferous epithelium of the testis, is involved in regulating pituitary gonadotropin secretion, and possibly involved in intragonadal regulation as well...
  89. OPTIMIZING SPERMATOGONIAL TRANSPLANTATION FOR FERTILITY PRESERVATION
    Gunapala Shetty; Fiscal Year: 2010
    ..from the transplanted stem spermatogonia, especially considering the potential adverse effects of cytotoxicants on testis. Previously we demonstrated that in rats testicular irradiation damages the somatic environment resulting in a ..
  90. Arthur Louis Burnett; Fiscal Year: 2015
    ..The proposal fits with goals of the funding agency pertaining to reproductive biology and testis function.
  91. REGULATION AND FUNCTION OF SPARC IN MALE REPRODUCTION
    Robert Vernon; Fiscal Year: 1991
    Spermatogenesis in the mammalian testis is regulated in part by a complex set of interactions among the nongerminal Sertoli, Leydig, and peritubular cells that create a unique environment for the maturation of spermatozoa...
  92. REGULATION AND FUNCTION OF HEAT SHOCK FACTORS IN TESTIS
    KEVIN SARGE; Fiscal Year: 1999
    ..HSF2 expression is regulated in germ cells, that the HSF2 protein exhibits constitutive DNA-binding activity in testis, and that HSF2 interacts with sequences in the hsp70.2 gene promoter...
  93. EFFECTS OF AGING ON GERMLINE AND SOMATIC STEM CELLS IN DROSOPHILA MELANOGASTER
    MATTHEW WALLENFANG; Fiscal Year: 2007
    ..The research proposed examines the effect of aging on stem cells in the Drosophila testis, which presents 3 major advantages for this type of study...
  94. ANALYSIS OF THE ROLE OF GCNF IN SPERMATOGENESIS
    Austin Cooney; Fiscal Year: 1999
    ..Within the testis control involves many paracrine signaling pathways primarily emanating in the Leydig or Sertoli cells...
  95. PRO-OPIOMELANOCORTIN SYNTHESIS IN TESTES
    Nathaniel Mills; Fiscal Year: 1992
    ..Regulation of POMC synthesis in the gonad may be very important and may provide insights for overall control of spermatogenesis...
  96. CELL-CELL INTERACTIONS IN TESTIS DEVELOPMENT
    Michael Skinner; Fiscal Year: 2007
    The proposed research is designed to develop a better understanding of the mechanisms that control and maintain testis development and function...
  97. Reversible vitamin A-dependent arrest of spermatogonia
    JAMES TSURUTA; Fiscal Year: 2007
    ..of contraceptive strategies based on the reversible arrest of spermatogonia (Spg) in the vitamin A-deficient (VAD) testis. Our intent is to uncover specific mechanisms that reversibly arrest Spg in this model...
  98. SERTOLI CELL-GERM CELL SURFACE MEMBRANE INTERACTIONS
    ROBERT DEPHILIP; Fiscal Year: 1991
    ..here is the identification of mechanisms used by the Sertoli cell to regulate germ cell development in the testis. Of particular interest are processes and specific molecules that operate at the level of the plasma membrane ..
  99. MECHANISMS OF TOXICITY IN TESTES
    Michael Welsh; Fiscal Year: 1999
    ..Hsp27 also co- localizes with microfilaments in Sertoli cells (SC) of the testis. We hypothesize that hsp27 regulates SC microfilaments, and that toxicants disrupt spermatogenesis by altering ..