vagina

Summary

Summary: The genital canal in the female, extending from the UTERUS to the VULVA. (Stedman, 25th ed)

Top Publications

  1. pmc Vaginal microbiome of reproductive-age women
    Jacques Ravel
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 108:4680-7. 2011
  2. pmc Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria
    Sujatha Srinivasan
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS ONE 7:e37818. 2012
  3. pmc Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis
    Zongxin Ling
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China
    BMC Genomics 11:488. 2010
  4. pmc Glycerol monolaurate prevents mucosal SIV transmission
    Qingsheng Li
    Department of Microbiology, Medical School, University of Minnesota, MMC 196, 420 Delaware Street S E, Minneapolis, Minnesota 55455, USA
    Nature 458:1034-8. 2009
  5. pmc CD8(+) T lymphocyte mobilization to virus-infected tissue requires CD4(+) T-cell help
    Yusuke Nakanishi
    Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 462:510-3. 2009
  6. ncbi Molecular identification of bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    N Engl J Med 353:1899-911. 2005
  7. pmc Deep sequencing of the vaginal microbiota of women with HIV
    Ruben Hummelen
    Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, London, Canada
    PLoS ONE 5:e12078. 2010
  8. ncbi Early events in sexual transmission of HIV and SIV and opportunities for interventions
    Ashley T Haase
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Annu Rev Med 62:127-39. 2011
  9. pmc Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:16. 2004
  10. pmc Molecular definition of vaginal microbiota in East African commercial sex workers
    John J Schellenberg
    Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Appl Environ Microbiol 77:4066-74. 2011

Detail Information

Publications310 found, 100 shown here

  1. pmc Vaginal microbiome of reproductive-age women
    Jacques Ravel
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 108:4680-7. 2011
    ....
  2. pmc Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria
    Sujatha Srinivasan
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS ONE 7:e37818. 2012
    ..We investigated the associations between the presence of individual bacterial species and clinical diagnostic characteristics of BV...
  3. pmc Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis
    Zongxin Ling
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China
    BMC Genomics 11:488. 2010
    ....
  4. pmc Glycerol monolaurate prevents mucosal SIV transmission
    Qingsheng Li
    Department of Microbiology, Medical School, University of Minnesota, MMC 196, 420 Delaware Street S E, Minneapolis, Minnesota 55455, USA
    Nature 458:1034-8. 2009
    ..by these cells, in combination with the innate immune and inflammatory responses to infection in both cervix and vagina, recruits CD4(+) T cells to fuel this obligate expansion...
  5. pmc CD8(+) T lymphocyte mobilization to virus-infected tissue requires CD4(+) T-cell help
    Yusuke Nakanishi
    Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 462:510-3. 2009
    ..Our results reveal a previously unappreciated role of CD4 help in mobilizing effector CTL to the peripheral sites of infection where they help to eliminate infected cells...
  6. ncbi Molecular identification of bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    N Engl J Med 353:1899-911. 2005
    ..The cause of bacterial vaginosis remains poorly understood despite numerous studies based on cultures. Bacteria in microbial communities can be identified without cultivation by characterizing their ribosomal DNA (rDNA) sequences...
  7. pmc Deep sequencing of the vaginal microbiota of women with HIV
    Ruben Hummelen
    Canadian Research and Development Centre for Probiotics, Lawson Health Research Institute, London, Canada
    PLoS ONE 5:e12078. 2010
    ..It is poorly understood which bacterial communities constitute BV or the normal vaginal microbiota among this population and how the microbiota associated with BV responds to antibiotic treatment...
  8. ncbi Early events in sexual transmission of HIV and SIV and opportunities for interventions
    Ashley T Haase
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Annu Rev Med 62:127-39. 2011
    ....
  9. pmc Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis
    Rita Verhelst
    Department Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium
    BMC Microbiol 4:16. 2004
    ..Here, we report on the results obtained by combining culture and PCR-based methods to characterize the normal and disturbed vaginal microflora...
  10. pmc Molecular definition of vaginal microbiota in East African commercial sex workers
    John J Schellenberg
    Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada
    Appl Environ Microbiol 77:4066-74. 2011
    ..We conclude that cpn60 UT is ideally suited to next-generation sequencing technologies for further investigation of microbial community dynamics and mucosal immunity underlying HIV resistance in this cohort...
  11. ncbi Female genital-tract HIV load correlates inversely with Lactobacillus species but positively with bacterial vaginosis and Mycoplasma hominis
    Beverly E Sha
    Department of Medicine, Section of Infectious Diseases, Rush University Medical Center, University of Illinois at Chicago, Illinois, USA
    J Infect Dis 191:25-32. 2005
    ..Bacterial vaginosis (BV) is associated with human immunodeficiency virus (HIV) acquisition. We examined the association between BV and BV-associated bacteria and expression of HIV in the female genital tract...
  12. pmc Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora
    Hans Verstraelen
    Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    BMC Microbiol 9:116. 2009
    ..their antimicrobial potential, vaginal lactobacilli often fail to retain dominance, resulting in overgrowth of the vagina by other bacteria, as observed with bacterial vaginosis...
  13. pmc Quantification of bacterial species of the vaginal microbiome in different groups of women, using nucleic acid amplification tests
    Vicky Jespers
    Department of Public Health, ITM HIV AIDS Centre, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium
    BMC Microbiol 12:83. 2012
    ..We also explored differences in qPCR data between these healthy women and an 'at risk' clinic population of Caucasian, African and Asian women with and without bacterial vaginosis (BV), as defined by the Nugent score...
  14. pmc Oral pre-exposure prophylaxis by anti-retrovirals raltegravir and maraviroc protects against HIV-1 vaginal transmission in a humanized mouse model
    C Preston Neff
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS ONE 5:e15257. 2010
    ..Based on these results both these drugs show great promise for further development as orally administered PrEPs...
  15. pmc Anti-HIV activity in cervical-vaginal secretions from HIV-positive and -negative women correlate with innate antimicrobial levels and IgG antibodies
    Mimi Ghosh
    Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire, United States of America
    PLoS ONE 5:e11366. 2010
    ..Since female reproductive tract (FRT) secretions contain a spectrum of antimicrobials, we hypothesized that CVL from healthy HIV(+) and (-) women inhibit HIV infection...
  16. pmc Comparison of the diversity of the vaginal microbiota in HIV-infected and HIV-uninfected women with or without bacterial vaginosis
    Gregory T Spear
    Department of Immunology Microbiology, Rush University Medical Center, Chicago, Illinois, USA
    J Infect Dis 198:1131-40. 2008
    ..Whether human immunodeficiency virus (HIV) infection is associated with a change in the diversity of genital microbiota in women was investigated...
  17. pmc Vaginal lactobacillus flora of healthy Swedish women
    Alejandra Vásquez
    Laboratory of Food Hygiene, Division of Food Technology, Lund University, Sweden
    J Clin Microbiol 40:2746-9. 2002
    ..L. iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.
  18. pmc Quantitative determination by real-time PCR of four vaginal Lactobacillus species, Gardnerella vaginalis and Atopobium vaginae indicates an inverse relationship between L. gasseri and L. iners
    Ellen De Backer
    Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, UGent, Ghent, Belgium
    BMC Microbiol 7:115. 2007
    ..iners and L. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years...
  19. ncbi Inhibiting sexual transmission of HIV-1 infection
    Robin J Shattock
    Department of Cellular and Molecular Medicine, Infectious Diseases, St George s Hospital Medical School, London, UK
    Nat Rev Microbiol 1:25-34. 2003
    ....
  20. ncbi A vaginal fluid simulant
    D H Owen
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Contraception 59:91-5. 1999
    A fluid medium was developed to simulate the fluid produced in the human vagina. The composition of the medium was based on an extensive review of the literature on constituents of human vaginal secretions...
  21. ncbi The molecular basis of nonoxynol-9-induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis St, Thorn 217, Boston, MA 02115, USA
    J Infect Dis 184:418-28. 2001
    ..Furthermore, this study identifies in vitro and in vivo model systems for monitoring undesirable proinflammatory effects of microbicides and other vaginal products...
  22. pmc Vaginal microbicides: detecting toxicities in vivo that paradoxically increase pathogen transmission
    Richard A Cone
    Mucosal Protection Laboratory, Dept of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA
    BMC Infect Dis 6:90. 2006
    ....
  23. pmc The vaginal microbiome: new information about genital tract flora using molecular based techniques
    R F Lamont
    Perinatology Research Branch, National Institute of Child Health and Human Development, NIH DHHS, Bethesda, MD 20892, USA
    BJOG 118:533-49. 2011
    ..This information may help to elucidate the link between BV and infection-related adverse outcomes of pregnancy...
  24. pmc Quantitative PCR assessments of bacterial species in women with and without bacterial vaginosis
    Marcela Zozaya-Hinchliffe
    The Research Institute for Children, Children s Hospital, Department of Pediatrics and Department of Internal Medicine, Louisiana State University Health Sciences Center, 200 Henry Clay Ave, New Orleans, LA 70118, USA
    J Clin Microbiol 48:1812-9. 2010
    ..qPCR assays can be a useful tool for describing the structure of vaginal communities and elucidating their role in health and disease...
  25. pmc Longitudinal qPCR study of the dynamics of L. crispatus, L. iners, A. vaginae, (sialidase positive) G. vaginalis, and P. bivia in the vagina
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 7:e45281. 2012
    ..To obtain more detailed understanding of the causes of disturbance of the vaginal microflora (VMF), a longitudinal study was carried out for 17 women during two menstrual cycles...
  26. pmc Novel vaginal microflora colonization model providing new insight into microbicide mechanism of action
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, Massachusetts, USA
    MBio 2:e00168-11. 2011
    ....
  27. pmc Longitudinal study of the dynamics of vaginal microflora during two consecutive menstrual cycles
    Guido Lopes Dos Santos Santiago
    Laboratory for Bacteriology Research, Department of Clinical Chemistry, Microbiology, and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    PLoS ONE 6:e28180. 2011
    ....
  28. ncbi High susceptibility to repeated, low-dose, vaginal SHIV exposure late in the luteal phase of the menstrual cycle of pigtail macaques
    Sundaram A Vishwanathan
    Division of HIV AIDS Prevention, CDC, Atlanta, GA 30329, USA
    J Acquir Immune Defic Syndr 57:261-4. 2011
    ..Our data support the findings of higher susceptibility to HIV in women during progesterone-dominated periods including pregnancy and contraceptive use...
  29. pmc Progesterone increases susceptibility and decreases immune responses to genital herpes infection
    Charu Kaushic
    Department of Pathology and Molecular Medicine, Center of Gene Therapeutics, McMaster University Health Sciences Center, Hamilton, Ontario, Canada
    J Virol 77:4558-65. 2003
    ..Animal models and vaccine strategies for sexually transmitted diseases need to consider the effect of hormone treatments on susceptibility and immune responses...
  30. ncbi Protection of macaques against vaginal SHIV challenge by systemic or mucosal and systemic vaccinations with HIV-envelope
    Susan W Barnett
    Novartis Vaccines and Diagnostics, Inc, 4560 Horton Street, Emeryville, California 94608, USA
    AIDS 22:339-48. 2008
    ..Worldwide, the majority of human immunodeficiency virus (HIV) infections occur by heterosexual transmission. Thus, the development of a vaccine that can prevent intravaginal HIV infection is an important goal of AIDS vaccine research...
  31. pmc Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis
    Brian B Oakley
    Program in Infectious Diseases, D3 100, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109 1024, USA
    Appl Environ Microbiol 74:4898-909. 2008
    ..Our data describe a previously unrecognized extent of bacterial diversity in the vaginal ecosystem. The human vagina hosts many bacteria that are only distantly related to known species, and subjects with BV harbor particularly ..
  32. pmc Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue, North, D3 100, Box 19024, Seattle, WA 98109 1024, USA
    J Clin Microbiol 45:3270-6. 2007
    ..We sought to determine the prevalence of each species in the vagina, its association with BV, and the utility of PCR for the microbiological diagnosis of BV...
  33. pmc At the crossroads of vaginal health and disease, the genome sequence of Lactobacillus iners AB-1
    Jean M Macklaim
    Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute, London, ON, Canada
    Proc Natl Acad Sci U S A 108:4688-95. 2011
    Lactobacilli have long been regarded as important constituents of the healthy human vagina. Lactobacillus iners is the most frequently detected bacterial species in the vagina, but little is known about its characteristics...
  34. pmc Dendritic cells and B cells maximize mucosal Th1 memory response to herpes simplex virus
    Norifumi Iijima
    Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 205:3041-52. 2008
    ..Thus, these results revealed a distinct mechanism by which memory Th1 cells mediate noncytolytic IFN-gamma-dependent antiviral protection after recognition of processed viral antigens by local DCs and B cells...
  35. ncbi Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Reprod 60:508-14. 1999
    We have recently generated human papillomavirus (HPV) 16/E6E7 immortalized epithelial cell lines from the human vagina, ectocervix, and endocervix to use in studies on the role of these cells in reproduction and immune defense...
  36. pmc HIV binding, penetration, and primary infection in human cervicovaginal tissue
    Diane Maher
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware Street Southeast, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 102:11504-9. 2005
    ..Collectively, these results highlight a continuum of complex interactions that occurs when natural sources of HIV infectivity are deposited onto mucosal surfaces in the female reproductive tract...
  37. ncbi PCR-based identification of eight Lactobacillus species and 18 hr-HPV genotypes in fixed cervical samples of South African women at risk of HIV and BV
    Joke A M Dols
    Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Diagn Cytopathol 40:472-7. 2012
    ..Because of the prominence of HPV35, HPV52, HPV33, and HPV66, vaccination for exclusively HPV16 and HPV18 might be insufficient in South African HIV+ women...
  38. ncbi Advances in development, scale-up and manufacturing of microbicide gels, films, and tablets
    Sanjay Garg
    School of Pharmacy, University of Auckland, 93 Grafton Road, Grafton, Auckland, New Zealand
    Antiviral Res 88:S19-29. 2010
    ..This article forms part of a special supplement covering presentations on gels, tablets, and films from the symposium on "Recent Trends in Microbicide Formulations" held on 25 and 26 January 2010, Arlington, VA...
  39. pmc Bacterial vaginosis (BV) candidate bacteria: associations with BV and behavioural practices in sexually-experienced and inexperienced women
    Katherine Fethers
    School of Population Health, University of Melbourne, Melbourne, Victoria, Australia
    PLoS ONE 7:e30633. 2012
    ....
  40. pmc Performance of swabs, lavage, and diluents to quantify biomarkers of female genital tract soluble mucosal mediators
    Charlene S Dezzutti
    Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e23136. 2011
    ..However, optimal methods for sample collection do not exist. This study compared collection methods...
  41. pmc Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres
    Tim J Dumonceaux
    National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, 1015 Arlington St, Winnipeg, MB, Canada
    J Clin Microbiol 47:4067-77. 2009
    ..Using the presence of A. vaginae or G. vaginalis, or both, as the defining criterion for BV, we found that the method was highly specific and sensitive for the diagnosis of BV using microscopy as a gold standard...
  42. pmc The vaginal bacterial communities of Japanese women resemble those of women in other racial groups
    Xia Zhou
    Department of Biological Sciences, University of Idaho, Moscow, ID, USA
    FEMS Immunol Med Microbiol 58:169-81. 2010
    ....
  43. pmc Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    C A Gaydos
    Johns Hopkins University, 855 North Wolfe St, 530 Rangos Building, Baltimore, MD 21205, USA
    J Clin Microbiol 48:3236-43. 2010
    ..trachomatis and N. gonorrhoeae dual detection with high sensitivity and specificity. The automated assay provides a useful alternative nucleic acid amplification assay for clinical laboratories and clinicians...
  44. ncbi The lower genital tract microbiota in relation to cytokine-, SLPI- and endotoxin levels: application of checkerboard DNA-DNA hybridization (CDH)
    Natalia Nikolaitchouk
    Department of Clinical Bacteriology, Gothenburg University, Gothenburg, Sweden
    APMIS 116:263-77. 2008
    ..CDH appears to be a suitable tool for future analyses of large numbers of clinical samples with an extended number of bacterial probes...
  45. pmc Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR
    David N Fredricks
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Clin Microbiol 47:721-6. 2009
    ..DNA from several fastidious BV-associated bacteria (BVAB) were present at high concentrations in the vagina prior to treatment...
  46. pmc Trichomonas vaginalis polyamine metabolism is linked to host cell adherence and cytotoxicity
    Ana F Garcia
    Department Microbiology, UTHSCSA, 7703 Floyd Curl Dr, San Antonio, TX 78229 3900, USA
    Infect Immun 73:2602-10. 2005
    ..Overall, these data suggest that polyamine metabolism and secreted putrescine are linked to host cell adherence and cytotoxicity...
  47. ncbi Inverse association of H2O2-producing lactobacilli and vaginal Escherichia coli colonization in women with recurrent urinary tract infections
    K Gupta
    Department of Medicine, University of Washington, Seattle 98195, USA
    J Infect Dis 178:446-50. 1998
    ..5; P = .02). Thus, absence of H2O2-positive lactobacilli may be important in the pathogenesis of recurrent UTI by facilitating E. coli introital colonization...
  48. ncbi High recurrence rates of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence
    Catriona S Bradshaw
    Melbourne Sexual Health Centre, The Alfred Hospital, Victoria, Australia
    J Infect Dis 193:1478-86. 2006
    ..We wished to determine recurrences of bacterial vaginosis (BV) after treatment over the course of 12 months and to establish factors associated with recurrence...
  49. pmc Human immunodeficiency virus type 1 is trapped by acidic but not by neutralized human cervicovaginal mucus
    Samuel K Lai
    Department of Chemical and Biomolecular Engineering, Institute for NanoBioTechnology, Johns Hopkins University, 3400 N Charles Street, Baltimore, Maryland 21218, USA
    J Virol 83:11196-200. 2009
    ..In contrast, in CVM neutralized to pH 6 to 7, as occurs when semen temporarily neutralizes the vagina, HIV maintained its native surface charge and diffused only 15-fold more slowly than it would in water...
  50. ncbi Prevalence and impact of vaginal symptoms among postmenopausal women
    Nanette Santoro
    Division of Reproductive Endocrinology, Albert Einstein College of Medicine, Ob Gyn and Women s Health, New York, NY 10461, USA
    J Sex Med 6:2133-42. 2009
    ..Vulvovaginal atrophy (VVA) is reported by one-quarter to one-half of postmenopausal women...
  51. ncbi Seminal plasma differentially regulates inflammatory cytokine gene expression in human cervical and vaginal epithelial cells
    David J Sharkey
    Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide SA 5005, Australia
    Mol Hum Reprod 13:491-501. 2007
    ..Seminal factor regulation of inflammatory cytokines in the cervical epithelium is implicated in controlling the immune response to seminal antigens, and defence against infectious agents introduced at intercourse...
  52. ncbi Women find it easy and prefer to collect their own vaginal swabs to diagnose Chlamydia trachomatis or Neisseria gonorrhoeae infections
    Max A Chernesky
    St Joseph s Healthcare, Hamilton, Ontario, Canada
    Sex Transm Dis 32:729-33. 2005
    ..We surveyed women's opinions on ease and preferences as to sampling after collecting their own vaginal swab and urine and a physician collection of vaginal swab and cervical swab...
  53. ncbi Failure of metronidazole to prevent preterm delivery among pregnant women with asymptomatic Trichomonas vaginalis infection
    M A Klebanoff
    National Institute of Child Health and Human Development, Bethesda, MD 20892 7510, USA
    N Engl J Med 345:487-93. 2001
    ..Infection with Trichomonas vaginalis during pregnancy has been associated with preterm delivery. It is uncertain whether treatment of asymptomatic trichomoniasis in pregnant women reduces the occurrence of preterm delivery...
  54. ncbi Diversity of cervicovaginal microbiota associated with female lower genital tract infections
    Zongxin Ling
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China
    Microb Ecol 61:704-14. 2011
    ..Previous studies have focused on bacterial diversity in the vagina, but little is known about the ectocervical microbiota associated with FGT infections...
  55. pmc Vaccination with the Chlamydia trachomatis major outer membrane protein can elicit an immune response as protective as that resulting from inoculation with live bacteria
    Sukumar Pal
    Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, CA 92697 4800, USA
    Infect Immun 73:8153-60. 2005
    ..05). In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice...
  56. pmc Pyrosequencing of the genital microbiotas of HIV-seropositive and -seronegative women reveals Lactobacillus iners as the predominant Lactobacillus Species
    Gregory T Spear
    Department of Immunology Microbiology, Rush University Medical Center, Chicago, IL 60612, USA
    Appl Environ Microbiol 77:378-81. 2011
    ..Lactobacillus iners sequences were the predominant lactobacillus sequences in 66% of HIV(+) women and 90% of HIV(-) women. This has implications for resistance of HIV(+) and HIV(-) women to genital colonization by pathogenic organisms...
  57. ncbi Initiation of antiretroviral therapy leads to a rapid decline in cervical and vaginal HIV-1 shedding
    Susan M Graham
    Department of Medicine, University of Washington, Seattle, Washington, USA
    AIDS 21:501-7. 2007
    ..Antiretroviral therapy (ART) may decrease HIV-1 infectivity in women by reducing genital HIV-1 shedding...
  58. pmc Heterogeneity of vaginal microbial communities within individuals
    Tae Kyung Kim
    Host Microbe Systems Theme, Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Clin Microbiol 47:1181-9. 2009
    ..Thus, this study illuminates the complex structure of the vaginal ecosystem and calls for the consideration of microenvironments when sampling vaginal microbiota as a clinical predictor of vaginal health...
  59. ncbi Disentangling contributions of reproductive tract infections to HIV acquisition in African Women
    Janneke H H M van de Wijgert
    Academic Medical Center, Center for Poverty related Communicable Diseases, Meibergdreef 9 T0 120, PO Box 22700, 1100 DE Amsterdam, The Netherlands
    Sex Transm Dis 36:357-64. 2009
    ..To estimate the effects of reproductive tract infections (RTIs) on HIV acquisition among Zimbabwean and Ugandan women...
  60. pmc Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Brigham and Women s Hospital, 221 Longwood Avenue, RF468, Boston, MA 02115, USA
    Infect Immun 74:5773-9. 2006
    ..These effects were dose dependent and sustained in the absence of cytotoxicity and IL-1beta release and utilized, at least in part, a signaling pathway independent from the Toll-like/IL-1 receptor adaptor protein MyD88...
  61. pmc Rapid fluctuation of the vaginal microbiota measured by Gram stain analysis
    Rebecca M Brotman
    Department of Epidemiology and Preventive Medicine, Institute for Genome Sciences, University of Maryland School of Medicine, BioPark Building II, 801 West Baltimore Street, Baltimore, MD 21201, USA
    Sex Transm Infect 86:297-302. 2010
    ..The aetiology of bacterial vaginosis (BV) remains unknown...
  62. pmc Comparison of self-collected and physician-collected vaginal swabs for microbiome analysis
    Larry J Forney
    Department fo Biological Sciences, University of Idaho, Moscow, ID 83844 3051, USA
    J Clin Microbiol 48:1741-8. 2010
    ..and then were examined by a physician, who collected an additional five swabs from the lateral wall of the mid-vagina. In this study, the self-collected and three physician-obtained swabs were analyzed and compared using terminal ..
  63. pmc Use of human tissue explants to study human infectious agents
    Jean Charles Grivel
    Section of Intercellular Interactions, Program on Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, 10 Centre Drive, Room 9D58, Bethesda, Maryland 20892, USA
    Nat Protoc 4:256-69. 2009
    ..Using this protocol, culture and infections can be set up in 3-6 h and be maintained for 2-3 weeks, depending on the tissue used...
  64. ncbi Dissimilarity in the occurrence of Bifidobacteriaceae in vaginal and perianal microbiota in women with bacterial vaginosis
    Alexander Swidsinski
    Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms, 10098 Berlin, Germany
    Anaerobe 16:478-82. 2010
    ..Only some species originating from the intestinal tract do display pronounced vaginotropism, like G. vaginalis, whereas many other species do not...
  65. pmc Vaginal practices diary: development of a pictorial data collection tool for sensitive behavioral data
    Suzanna C Francis
    London School of Hygiene and Tropical Medicine, London, United Kingdom
    Sex Transm Dis 39:614-21. 2012
    ..Daily self-administered diaries may help to decrease bias associated with FTFI. IVP data from a diary and FTFI were compared during a multisite microbicide feasibility study in Tanzania and Uganda...
  66. ncbi Characterisation of the oral, vaginal and rectal Lactobacillus flora in healthy pregnant and postmenopausal women
    Ljubomir Petricevic
    Department of Obstetrics and Fetomaternal Medicine, Medical University of Vienna, Waehringer Guertel 18 20, A 1090 Vienna, Austria
    Eur J Obstet Gynecol Reprod Biol 160:93-9. 2012
    ..To investigate the hypothesis that the rectum may be an important reservoir for vaginal colonisation by Lactobacillus species...
  67. pmc Intravaginal practices, vaginal infections and HIV acquisition: systematic review and meta-analysis
    Adriane Martin Hilber
    Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 5:e9119. 2010
    ..The objectives of this study were to examine evidence for associations between: intravaginal practices and acquisition of HIV infection; intravaginal practices and vaginal infections; and vaginal infections and HIV acquisition...
  68. ncbi Infection of macaques after vaginal exposure to cell-associated simian immunodeficiency virus
    Bettina Salle
    Commissariat a l Energie Atomique CEA, Division of Immuno Virology, Institute of Emerging Diseases and Innovative Therapies IMETI, Fontenay aux Roses, France
    J Infect Dis 202:337-44. 2010
    ..The contribution of infected semen cells to sexual transmission of human immunodeficiency virus (HIV) is still debated. We addressed this issue in the model of experimental infection of macaques with simian immunodeficiency virus (SIV)...
  69. pmc Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis
    Charles P Cartwright
    ViroMed Laboratories Inc, Minnetonka, Minnesota, USA
    J Clin Microbiol 50:2321-9. 2012
    ....
  70. pmc Reformulated tenofovir gel for use as a dual compartment microbicide
    Charlene S Dezzutti
    University of Pittsburgh, Pittsburgh, PA 15213, USA
    J Antimicrob Chemother 67:2139-42. 2012
    ..This study evaluated the pre-clinical safety and efficacy of a reformulated (reduced osmolality) tenofovir gel product...
  71. ncbi Remodeling of vaginal connective tissue in patients with prolapse
    Pamela A Moalli
    Magee Womens Research Institute and the Department of Obstetrics and Gynecology at Magee Womens Hospital, 300 Halket Street, Pittsburgh, PA 15213, USA
    Obstet Gynecol 106:953-63. 2005
    As pelvic organ prolapse progresses, the morphology of the vagina dramatically changes...
  72. pmc Prevalence and abundance of uncultivated Megasphaera-like bacteria in the human vaginal environment
    Marcela Zozaya-Hinchliffe
    The Research Institute for Children, 1 New Orleans, Louisiana, USA
    Appl Environ Microbiol 74:1656-9. 2008
    ..Quantitative PCR suggests that both phylotypes are present in higher concentrations in women with bacterial vaginosis...
  73. pmc Pelvic organ prolapse in fibulin-5 knockout mice: pregnancy-induced changes in elastic fiber homeostasis in mouse vagina
    Peter G Drewes
    Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9032, USA
    Am J Pathol 170:578-89. 2007
    ..pelvic organ support was characterized in Fbln5-/- mice, and changes in elastic fiber homeostasis in the mouse vagina during pregnancy and parturition were determined...
  74. ncbi Pharmacokinetic assessment of dapivirine vaginal microbicide gel in healthy, HIV-negative women
    Annalene M Nel
    International Partnership for Microbicides, Silver Spring, Maryland 20910, USA
    AIDS Res Hum Retroviruses 26:1181-90. 2010
    ..6-8.3 × 10(6) pg/ml on Day 1 and from 2.3-20.7 × 10(6) pg/ml on Day 10 across dose groups. Dapivirine was detectable in all tissue biopsies on Day 10 at concentrations of 1.0-356 × 10(3) pg/mg...
  75. pmc The vaginal microbiome in health and disease
    Bryan A White
    Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA
    Trends Endocrinol Metab 22:389-93. 2011
    ....
  76. pmc Novel toxic shock syndrome toxin-1 amino acids required for biological activity
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 47:12995-3003. 2008
    ..Toxic shock syndrome toxin-1 residue D130 may contribute to binding an epithelial receptor, which allows it to penetrate the vaginal mucosa, induce interleukin-8, and cause toxic shock syndrome...
  77. ncbi Influence of lactic acid on endogenous and viral RNA-induced immune mediator production by vaginal epithelial cells
    Hannah Mossop
    Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA
    Obstet Gynecol 118:840-6. 2011
    ..To evaluate the influence of lactic acid on immune mediator release from vaginal epithelial cells...
  78. pmc Composition of the vaginal microbiota in women of reproductive age--sensitive and specific molecular diagnosis of bacterial vaginosis is possible?
    Elena Shipitsyna
    Laboratory of Microbiology, D O Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia
    PLoS ONE 8:e60670. 2013
    ..This study aimed to investigate the composition of the vaginal microbiota in women of reproductive age (healthy women and women with BV), with the view of developing molecular criteria for BV diagnosis...
  79. ncbi Recurrent aberrations identified by array-CGH in patients with Mayer-Rokitansky-Küster-Hauser syndrome
    Susanne Ledig
    Institut fur Humangenetik, Westfalische Wilhelms Universitat, Munster, Germany
    Fertil Steril 95:1589-94. 2011
    ..To identify genetic causes of Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome...
  80. ncbi Biomechanical properties of prolapsed or non-prolapsed vaginal tissue: impact on genital prolapse surgery
    Clay Jean-Charles
    Clinique de chirurgie gynécologique, Hopital Jeanne de Flandre, CHRU de Lille, Lille, France
    Int Urogynecol J 21:1535-8. 2010
    ..Our aim is to characterize prolapsed and non-prolapsed vaginal tissue, and thus offer a better understanding of the genital prolapse physiopathology and an improvement of surgical treatments...
  81. ncbi Identification and characterization of the immunogenic cytotoxic TvCP39 proteinase gene of Trichomonas vaginalis
    Lucero de Los Ángeles Ramón-Luing
    Departamento de Infectómica y Patogénesis Molecular, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av IPN 2508, Col San Pedro Zacatenco, Delg Gustavo A Madero, CP 07360 Mexico, DF, Mexico
    Int J Biochem Cell Biol 43:1500-11. 2011
    ..In conclusion, our data support TvCP39 as one of the surface proteinases that is glycosylated and is involved in trichomonal cytotoxicity. Thus, TvCP39 is the first glycosylated cysteine proteinase detected in T. vaginalis...
  82. ncbi Preoperative work-up for patients with deeply infiltrating endometriosis: transvaginal ultrasonography must definitely be the first-line imaging examination
    Mathilde Piketty
    Department of Gynecology, Obstetrics II and Reproductive Medicine, Universite Paris Descartes, Paris, France
    Hum Reprod 24:602-7. 2009
    ..We compared the accuracy of TVUS and TRUS for diagnosing rectal wall involvement in patients presenting with histologically proved deeply infiltrating endometriosis (DIE)...
  83. pmc Pharmacokinetics of tenofovir following intravaginal and intrarectal administration of tenofovir gel to rhesus macaques
    Jeremy Nuttall
    International Partnership for Microbicides, Silver Spring, Maryland, USA
    Antimicrob Agents Chemother 56:103-9. 2012
    ..These data suggest that a tenofovir-based microbicide may have the potential to protect when exposure is via vaginal or anal intercourse, regardless of whether the microbicide is applied vaginally or rectally...
  84. ncbi Response of Gardnerella vaginalis biofilm to 5 days of moxifloxacin treatment
    Alexander Swidsinski
    Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms, Charite Universitatsmedizin Berlin, Berlin, Germany
    FEMS Immunol Med Microbiol 61:41-6. 2011
    ..Despite clear clinical efficacy, and initially definite suppression of the biofilm, moxifloxacin was, similar to metronidazole, not able to eradicate the Gardnerella vaginalis biofilm in all patients...
  85. pmc Looking ahead: a case for human papillomavirus testing of self-sampled vaginal specimens as a cervical cancer screening strategy
    Patti E Gravitt
    Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Int J Cancer 129:517-27. 2011
    ....
  86. ncbi Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
    Guido Lopes Dos Santos Santiago
    Department of Clinical Chemistry, Microbiology, and Immunology, Laboratory for Bacteriology Research, Faculty of Medicine and Health Sciences, Ghent University, Belgium
    Am J Obstet Gynecol 204:450.e1-7. 2011
    ..Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase...
  87. ncbi A randomized controlled safety trial of the diaphragm and cellulose sulfate microbicide gel in sexually active women in Zimbabwe
    Ariane van der Straten
    Department of Ob Gyn, University of California San Francisco, Women s Global Health Imperative, San Francisco, CA 94105, USA
    Contraception 76:389-99. 2007
    ..Cellulose sulfate (CS) is an antimicrobial and contraceptive agent. We assessed its safety when used alone or with the diaphragm in Harare, Zimbabwe...
  88. ncbi Comparison of BD GeneOhm real-time polymerase chain reaction with chromogenic and conventional culture methods for detection of group B Streptococcus in clinical samples
    Dawn Smith
    Microbiology Department, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK
    Diagn Microbiol Infect Dis 61:369-72. 2008
    ..5% versus 57% respectively, P < 0.02). After selective enrichment, 92.8% of GBS were isolated on chromID Strepto B compared with 89.2% isolated on sheep blood agar...
  89. pmc Extracellular matrix proteases contribute to progression of pelvic organ prolapse in mice and humans
    Madhusudhan Budatha
    Department of Molecular Biology, University of Texas, Southwestern Medical Center, Dallas, Texas, USA
    J Clin Invest 121:2048-59. 2011
    ..These results suggest that POP is an acquired disorder of extracellular matrix and that therapies targeting matrix proteases may be successful for preventing or ameliorating POP in women...
  90. ncbi Porcine vagina ex vivo as a model for studying permeability and pathogenesis in mucosa
    Christopher A Squier
    Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 54222, USA
    J Pharm Sci 97:9-21. 2008
    ..about the factors that should be considered when selecting the best model to study the permeability of the human vagina. The characteristics of an ex vivo porcine model are explored and the validity of this model is demonstrated in ..
  91. pmc The role of acidification in the inhibition of Neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth
    Michelle A Graver
    Medical Microbiology, King s College Hospital, Denmark Hill, London, UK
    Ann Clin Microbiol Antimicrob 10:8. 2011
    ..By co-cultivating N. gonorrhoeae in the presence of lactobacilli we sought to identify the relative contributions of acidification and hydrogen peroxide production to any growth inhibition of N. gonorrhoeae...
  92. pmc A live-attenuated HSV-2 ICP0 virus elicits 10 to 100 times greater protection against genital herpes than a glycoprotein D subunit vaccine
    William P Halford
    Department of Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield, Illinois, United States of America
    PLoS ONE 6:e17748. 2011
    ..significant resistance to HSV-2, only 3 of 45 gD-2-immunized mice survived an overwhelming challenge of the vagina or eyes with wild-type HSV-2 (MS strain)...
  93. pmc Th17 cells and IL-17 in protective immunity to vaginal candidiasis
    Donatella Pietrella
    Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Perugia, Italy
    PLoS ONE 6:e22770. 2011
    ..Th17 cells play a major role in coordinating the host defence in oropharyngeal candidiasis. In this study we investigated the involvement of the Th17 response in an animal model of vulvovaginal candidiasis (VVC)...
  94. ncbi Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathog
    Fabrice Atassi
    INSERM, UMR 756, Chatenay Malabry, France
    FEMS Microbiol Lett 304:29-38. 2010
    ..contributions of hydrogen peroxide and lactic acid in killing bacterial pathogens associated with the human vagina, urinary tract or intestine by two hydrogen peroxide-producing strains...
  95. ncbi The genital econiche: focus on microbiota and bacterial vaginosis
    Dan Danielsson
    Department of Clinical Microbiology and Immunology, Division of Laboratory Medicine, University Hospital, Orebro, Sweden
    Ann N Y Acad Sci 1230:48-58. 2011
    ..Gardnerella and Atopobium organisms attached to these clue cells can be demonstrated in the male genital econiche, likely reflecting a heterosexual transmission of the disorder...
  96. ncbi Placental histological inflammation and reproductive tract infections in a low risk pregnant population in Latvia
    Dace Rezeberga
    Department of Obstetrics and Gynecology, Riga Stradins University, Latvia, Belgium
    Acta Obstet Gynecol Scand 87:360-5. 2008
    ..To investigate the correlation of reproductive tract infections (RTI) and endogenous vaginal flora at first antenatal consultation with placental histological inflammation...
  97. pmc Mouse model of cervicovaginal toxicity and inflammation for preclinical evaluation of topical vaginal microbicides
    Bradley J Catalone
    Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    Antimicrob Agents Chemother 48:1837-47. 2004
    ..Collectively, these studies highlight the murine model of toxicity as a valuable tool for the preclinical assessment of toxicity and inflammation associated with exposure to candidate topical microbicides...
  98. pmc Mucosal transmission of R5 and X4 tropic HIV-1 via vaginal and rectal routes in humanized Rag2-/- gammac -/- (RAG-hu) mice
    Bradford K Berges
    Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA
    Virology 373:342-51. 2008
    ..in the rectal and vaginal mucosa and are susceptible to HIV-1 infection when exposed to cell-free HIV-1 either via vagina or rectum. Infection could be established without any prior hormonal conditioning or mucosal abrasion...
  99. ncbi Improved cure of bacterial vaginosis with single dose of tinidazole (2 g), Lactobacillus rhamnosus GR-1, and Lactobacillus reuteri RC-14: a randomized, double-blind, placebo-controlled trial
    Rafael C R Martinez
    Departamento de Analises Clinicas, Universidade de Sao Paulo, 14040 903 Ribeirao Preto, Brazil
    Can J Microbiol 55:133-8. 2009
    ..0% vs. 34.4% in the placebo group; p = 0.011). This study shows that probiotic lactobacilli can provide benefits to women being treated with antibiotics for an infectious condition...
  100. ncbi Transvaginal laparoscopically assisted endoscopic cholecystectomy: a hybrid approach to natural orifice surgery
    Marc Bessler
    Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Gastrointest Endosc 66:1243-5. 2007
  101. pmc Hydrogen peroxide-producing lactobacilli inhibit gonococci in vitro but not during experimental genital tract infection
    Dawn F Muench
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Infect Dis 199:1369-78. 2009
    ..gonorrhoeae balances the detrimental effects of H(2)O(2) during infection. We conclude that the association between lactobacilli and gonococci is complex and may be subject to factors that have not been reproduced in vitro...

Research Grants75

  1. Natural antimicrobials against bacterial vaginosis
    Michael Chikindas; Fiscal Year: 2006
    ..is a complex multi-microbial infection associated with the depletion of lactobacilli, the major flora of a healthy vagina and the overgrowth of Gardnerella vaginalis, Peptostreptococcus spp. and Prevotella bivia...
  2. Developing Physics-Based Virtual Simulation Technology for Natural Orifice Transl
    DANIEL BOUGERE JONES; Fiscal Year: 2013
    ..of laparoscopic surgery, where internal organs are accessed by perforating the viscera (stomach, colon or vagina) using a flexible endoscope inserted through natural orifices such as the mouth, anus or vagina;without making any ..
  3. The role of novel, uncultivable bacteria in incident PID
    CATHERINE L contact HAGGERTY; Fiscal Year: 2011
    ..are: to determine whether the presence and quantity of BV-associated bacteria and mycoplasmal bacteria in the vagina is predictive of incident PID;to determine the agreement between the presence of BV-associated bacteria and ..
  4. Development of transformed lactobacilli as a microbicide
    Richard B Markham; Fiscal Year: 2010
    ..5) Evaluate colonization of the mouse vagina and secretion of CD18-specific VHH by transformed S. gordonii in progesterone-treated Hu-PBL-SCID mice and by L...
  5. The Vaginal Microbiome: Disease, Genetics and the Environment
    GREGORY ALLEN BUCK; Fiscal Year: 2012
    DESCRIPTION: The vagina is an interactive interface with the environment, and as such is covered by a protective epithelial surface...
  6. Phenotype MicroArray Analysis of Fastidious Pathogens
    Barry Bochner; Fiscal Year: 2009
    ..pathogens (Helicobacter, Campylobacter, Arcobacter, Wolinella), and important colonizers of the colon and vagina (Bacteroides, Clostridium, Lactobacillus, Escherichia)...
  7. Molecular Epidemiology of the Vaginal Microbiome
    Rebecca M Brotman; Fiscal Year: 2013
    ..Disruption of the mutualistic relationship that exists between bacterial communities in the vagina and their hosts can lead to bacterial vaginosis (BV), a condition in which lactic acid-producing bacteria are ..
  8. The Role of Uncultivated Bacteria in Bacterial Vaginosis
    DAVID NEAL FREDRICKS; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The microbiota of the human vagina can profoundly affect the health of women and neonates...
  9. Functional Characterization of CXCL14, CXCL17, and CCL28; three mucosal chemokine
    Albert Zlotnik; Fiscal Year: 2010
    ..CCL28) that are highly expressed in mucosal tissues including those in various human body cavities (oral cavity, vagina, etc)...
  10. Vaginal microbiota, immune responses and vulvovaginal symptoms during menopause
    Rebecca M Brotman; Fiscal Year: 2013
    ..As women approach menopause, decreased estrogen levels are accompanied by changes in the vagina including decreased glycogen accumulation in the epithelium, increased pH, and deterioration of vaginal tissues, ..
  11. Novel Vaginal Microbicides Based On Stable AAV-Neutralizing Antibody Gene Transfe
    Wayne A Marasco; Fiscal Year: 2010
    ..We propose to develop a genetic microbicide that when delivered to the mucosal surface of the cervix and vagina will allow the lining cells to stably produce a neutralizing human anti-HIV antibody that blocks HIV-1 attachment ..
  12. Therapeutic vaccination against genital HPV infection
    Yung Nien Chang; Fiscal Year: 2013
    ..of HPV recombinant vaccinia triggers immune clearance using a new model of persistent HPV16 infection in the mouse vagina. These preclinical studies will drive future Papivax-driven clinical trials of therapeutic vaccines to eliminate ..
  13. Ex Vivo Female Reproductive Tract Integration In a 3D Microphysiologic System
    Teresa K Woodruff; Fiscal Year: 2013
    ..There are five main organs in the female reproductive tract-the ovary, fallopian tubes, uterus, cervix and vagina. Each organ is responsible for unique aspects of reproductive function, but act integrally to support overall ..
  14. Uterine-specific genetic modification and lymphangioleiomyomatosis
    JOSE M TEIXEIRA; Fiscal Year: 2012
    ..duct-derived internal female reproductive tract organs (uterus, oviduct, cervix, and cranial portion of the vagina) are the only structures from the bipotential mammalian embryo not found in males, suggesting that the hormonally ..
  15. Immune-pathophysiology of lymphocytic foci in Sjogren's syndrome
    Cuong Q Nguyen; Fiscal Year: 2010
    ..g., the Gl tract, skin, the lungs, the vasculature and muscular systems, kidneys, bladder and vagina), of particular interest are the various sensory, peripheral, cranial and myelopathic neuropathies that develop in ..
  16. The Microbial Ecology of Bacterial Vaginosis: A Fine Scale Resolution Metagenomic
    Jacques Ravel; Fiscal Year: 2011
    ..Disruption of the mutualistic relationship that exists between bacterial communities in the vagina and their hosts can lead to bacterial vaginosis (BV), a condition in which lactic acid producing bacteria are ..
  17. Mucosal immune response of the anus in women to HPV, intercourse, smoking and OCs
    ANNA BARBARA NONE MOSCICKI; Fiscal Year: 2010
    ..Over the past 15 years, we have attempted to address many of the immunologic questions in the cervix and vagina by incorporating immune assays in our ongoing longitudinal cohort: the natural history of HPV in teens (R37 ..
  18. Vaginal Ring for Preventing Bacterial Vaginosis
    THOMAS RAY MOENCH; Fiscal Year: 2010
    ..Vaginosis (BV) is an exceptionally common vaginal condition in which robust lactobacillus microflora in the vagina is replaced by a dense polymicrobial overgrowth. At any given time, about 1 in 3 women in the U.S...
  19. Pittsburgh Pelvic Floor Research Program
    Halina M Zyczynski; Fiscal Year: 2013
    ..in genomics, proteomics, tissue regenerative techniques, biochemical and biomechanical impact of meshes on the vagina and central neuronal control of bladder function...
  20. Comparative and Cost-Effectiveness of Preventing HPV-Related Diseases
    JANE JOOYUN KIM; Fiscal Year: 2013
    ..States;nearly 50% are cervical cancer, while the rest involve the oral cavity/oropharynx, anus, vulva, penis, and vagina. Collectively, these account for substantial morbidity and mortality, contribute to health disparities, and are ..
  21. Human Papillomavirus Infection in Perinatally HIV-infected Adolescents in Asia
    Annette H Sohn; Fiscal Year: 2013
    ..Detecting HPV infection in the cervix, vagina, anus, oral cavity, and male genitalia soon after sexual debut and monitoring virus clearance and cervical ..
  22. Primate Model of Mycoplasma genitalium
    Patricia A Totten; Fiscal Year: 2010
    ..genitalium to cause disease and persist in the urethra, vagina, cervix, and oviducts of these animals. Although other primate species have been less extensively studied, M...
  23. Duet cervical barrier device for maximizing protective efficacy of microbicides
    Richard Cone; Fiscal Year: 2009
    ..cervical barrier device that, unlike a diaphragm, delivers gel on both its upper and lower surfaces to protect the vagina as well as the cervix...
  24. Molecular etiology of cervicovaginal adenosis by in utero hormone exposure
    Takeshi Kurita; Fiscal Year: 2013
    ..as the presence of columnar (tall) glandular cells in normally squamous (flat) epithelium of ectocervix and vagina. Cervical/vaginal adenosis has been studied in the context of cervical/vaginal clear cell adenocaricinoma (CCAC) ..
  25. MOLECULAR AND CELLULAR TRANSPORT IN MUCUS
    W Mark Saltzman; Fiscal Year: 2012
    ..in the mouse;orally-administered polymer microparticles loaded with Vg can stimulate mucosal immunity in the vagina;surface modification of particles can influence local transport and antigen processing in dendritic cells;DNA ..
  26. Development of a Live Combinational Microbicide for Women
    Qiang Xu; Fiscal Year: 2010
    ..BV are associated with a depletion of hydrogen peroxide (H2O2)-producing lactobacilli that normally protect the vagina from infection by opportunistic pathogens...
  27. Co-factors in HIV Mucosal Infection
    Gregory A Viglianti; Fiscal Year: 2012
    ..of target cells and inflammation in vaginal and cervical tissue explants and in an organotypic model of the human vagina. PUBLIC HEALTH RELEVANCE: World-wide, most new infections with HIV-1, the virus that causes AIDS, occur in women ..
  28. Immuno-pathophysiology of Lymphocytic Foci in Sjogren?s Syndrome
    Cuong Q Nguyen; Fiscal Year: 2013
    ..can be systemic by affecting other organs including the Gl tract, skin, lungs, vasculature, kidneys, bladder and vagina. Involvement of the musculature can lead to flbromyalgla-llke symptoms and chronic fatigue...
  29. Effect of Lactobacillus on genital HIV-1 RNA and DNA shedding in women
    Jane E Hitti; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: The amount of HIV in the vagina and cervix is a major predictor of HIV transmission between sex partners and from mother to child...
  30. Development of Mental Health Treatment for Obstetric Fistula Patients
    Melissa H Watt; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Obstetric fistula is a hole between the bladder or rectum and the vagina, which develops when obstructed labor is not relieved by cesarean section and results in uncontrollable leaking of urine and/or ..
  31. Genetic Epidemiology of Pelvic Organ Prolapse
    Jennifer Marie Wu; Fiscal Year: 2010
    ..End-stage disease is characterized by complete protrusion of the uterus or vagina external to the body, resulting in a marked reduction in quality of life due to pelvic discomfort and bowel and ..
  32. Menstrual Cycle Control of HIV Infection in the Reproductive Tract
    CHARLES ROBERT WIRA; Fiscal Year: 2013
    ..and susceptibility to HIV infection of target cells (CD4+ T cells, macrophages and dendritic cells) in the vagina, cervix and uterus during the menstrual cycle;2) Examine the direct and indirect effects of sex hormones/..
  33. Symptoms of Lower Urinary Tract Dysfunction Research Network (LURN)
    Karl J Kreder; Fiscal Year: 2013
    ..g. bladder, urethra, vagina, prostate and/or pelvic floor muscle dysfunction), we hypothesize that many patients have central or systemic ..
  34. Vitamin D and the prevalence, incidence, and persistence of bacterial vaginosis
    Abigail Norris Turner; Fiscal Year: 2012
    ..vaginosis (BV) is a vaginal condition that develops when the concentration of healthy Lactobacillus species in the vagina declines and is replaced by other (largely anaerobic) bacterial species...
  35. Novel Determination of Microbicide PK in Women's Reproductive Health
    DAVID FRANK KATZ; Fiscal Year: 2013
    ..The instrument simultaneously measures local epithelial thickness distribution throughout the vagina - using customized, co-registered spectral domain optical coherence tomography, SD-OCT...
  36. Estrogenic Modulation of Genitourinary Epithelial-Probiotic Cross-Talk
    MICHAEL HARRISON HSIEH; Fiscal Year: 2013
    ..Gut-derived or sexually or vertically transmitted pathogens travel through the vagina to infect the female genitourinary tract...
  37. NMR studies of the effects of mode of birth on vaginal elastin
    Gregory Boutis; Fiscal Year: 2013
    ..of a number of human degenerative and genetic disorders, and is believed to play a critical role in the vagina and in the pathophysiology of pelvic organ prolapse...
  38. Advancing research on the sexually transmitted female 'nuisance' pathogen Trichom
    Jane Carlton; Fiscal Year: 2010
    ..these isolates to develop a standardized and accessible in vitro model system for the study of colonization of the vagina by the parasite...
  39. FEMALE REPRODUCTIVE TRACT DEVELOPMENT IN THE MOUSE
    Richard R Behringer; Fiscal Year: 2013
    Project Summary The mammalian female reproductive tract, including the oviducts, uterus, and vagina, is essential for the continuation of the species and a frequent site of human disease, including infertility and cancer...
  40. Targeting Dendritic Cell Subsets in Human Vagina to Elicit Local Immunity
    SangKon Oh; Fiscal Year: 2010
    ..Therefore, vaccines that induce potent mucosal immunity in the vagina have a high potential for the prevention and treatment of STDs...
  41. Development of Bacterial Therapeutics Targeting Bacterial Vaginosis-Associated Bi
    Qiang Xu; Fiscal Year: 2010
    ..is a complex polymicrobial syndrome resulting from the depletion of the normal H2O2-producing lactobacilli in the vagina, overgrowth of BV-associated microorganisms, and the establishment of an adherent Gardnerella/Atopobium-specific ..
  42. Elastic Fiber Homeostasis in the Vaginal Wall
    Hiromi Yanagisawa; Fiscal Year: 2013
    ..most prevalent pelvic floor disorders is pelvic organ prolapse, a condition in which the pelvic organs (bladder, vagina, cervix and uterus) herniate through the vaginal opening...
  43. POTENTIATING AN HIV DNA VACCINE WITH FLAGELLIN AT THE CERVICAL/VAGINAL MUCOSA
    Dinesh Singh; Fiscal Year: 2011
    ..flagellin enhances both innate and adaptive immunity, it can potentiate immunogenicity of this DNA at cervix and vagina. The approach is to use this non-infectious HIV-DNA along with flagellin at cervix and vagina of B ALB/c mice...
  44. Development of a Novel Semen-Activated Prodrug as an Anti-HIV Microbicide
    ROBERT WALTER BUCKHEIT; Fiscal Year: 2010
    ..prodrug form will be evaluated in specially designed in vitro assays to mimic the events which must occur in the vagina and to quantify the kinetics of drug activation and virus inactivation in the presence of semen and other ..
  45. Physical Activity and Pelvic Floor Disorders
    Ingrid E Nygaard; Fiscal Year: 2012
    ..urinary leakage and pelvic organ prolapse (dropping of pelvic organs such as the uterus into or out of the vagina) are very common...
  46. Novel Cultivation Methods for the Domestication of Vaginal Bacteria
    DAVID NEAL FREDRICKS; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The microbiota of the human vagina can profoundly affect the health of women and neonates...
  47. Vaginal Tactile Imager for Pelvic Floor Biomechanical Assessment
    Vladimir Egorov; Fiscal Year: 2013
    ..Clinical results demonstrated capability of the proposed approach for 3-D imaging of the vagina and pelvic floor support structures...
  48. Macrophage Phenotype as a Determinant of Outcome in Pelvic Organ Prolapse Repair
    BRYAN NICKLAUS BROWN; Fiscal Year: 2013
    ..There is a lack of rigorous scientific studies characterizing the effects of this host response in the vagina and the design of mesh materials largely relies on data generated in abdominal hernia repair...
  49. A Novel Approach for the Routine Screening for Ovarian Cancer
    Lewis K Pannell; Fiscal Year: 2013
    ..tubes and the uterus, there is a constant flow of mucus which has only one exit through the cervix and out the vagina. Proteins that are generated within the entire female reproductive system are trapped into this viscous fluid and ..
  50. Defensins in STI-Mediated Enhanced HIV Infectivity
    Theresa L Chang; Fiscal Year: 2013
    ..peptides in intestine, are constitutively expressed in Paneth cells but also found in the epithelium of the vagina and ectocervix. Induction of HD5 has been reported in the male urethra during C. trachomatis and N...
  51. Genomic Tools For Studying The Ecology Of The Human Vaginal Microflora
    Jacques Ravel; Fiscal Year: 2010
    ..Our long-term goal is to develop an accurate understanding of the composition and ecology of the vagina microbial ecosystem in normal, healthy women as an essential prerequisite for comprehending how the normal ..
  52. Methods to determine HIV Transport and Inactivation by Microbicide coating Layers
    DAVID FRANK KATZ; Fiscal Year: 2010
    ..This configuration simulates in vivo conditions in the vagina that, we hypothesize, play a critical role in microbicide efficacy;the configuration is not achieved in the few ..
  53. MR-Compatible X-Ray Tube
    Rebecca Fahrig; Fiscal Year: 2010
    ..5T GE Signa-SP open magnet (SP-XMR) for guiding a range of challenging procedures including creation of neo-vagina, and sclerotherapy for arteriovenous malformations...
  54. Mechanistic analysis of transport through the mucus barrier
    Katharina Ribbeck; Fiscal Year: 2013
    ..surface charge, hydrophobicity, and specific peptide sequences influences mucus transport in vivo, using the mouse vagina as a model...
  55. Eco-Pathogenomics of Chlamydial Reproductive Tract Infection (EPCRTI)
    Jacques Ravel; Fiscal Year: 2013
    The microbiota associated with the human vagina exists in a mutualistic relationship with the human host and is believed to play an important role in women's reproductive health...
  56. Glucan Binding to Azole Drugs: A Novel Resistance Mechanism in Candida albicans
    THEODORE WHITE; Fiscal Year: 2009
    ..Narrative The pathogenic yeast Candida albicans causes significant human disease in the mouth, vagina and blood stream. This proposal will analyze the interaction between the antifungal drug fluconazole and C...
  57. The Early Events Determining SIV Rectal Transmission
    Qingsheng Li; Fiscal Year: 2013
    ..Because the anatomical/histological features and the physiological functions of vagina and cervix differ from the rectum, we hypothesize that the underlying mechanisms and timing of HIV-1 rectal ..
  58. HIV-1 subtype B transmission in a heterosexual incident cohort
    Jean Carr; Fiscal Year: 2009
    ..Part of this fitness relates to matching inoculated viral trophisms to the HIV target cell in the vagina, rectum, or penis through established receptors such as DC-SIGN, CD4 and CCR-5...
  59. Development and Evaluation of Dual Compartment Combination Microbicides
    ROBERT WALTER BUCKHEIT; Fiscal Year: 2013
    ..between formulated microbicide products, virus, biological matrices, and target fluids and tissue in the vagina and rectum...
  60. Transcytosis of IgG in Genital Infections
    Xiaoping Zhu; Fiscal Year: 2010
    ..in epithelial cells derived from the human female genital tract, 3) FcRn binds IgG only at acidic pH;whereas, the vagina exhibits acidic pH, 4) the levels of IgG in the female genital tract can be changed over the course of the estrous ..
  61. Mechanisms of mucosal protection by HPV-SIV and gp96-lg-SIV vaccines
    Eckhard R Podack; Fiscal Year: 2011
    ..and with gp96-lg-SIV and determine the immune response, transcriptional profiles and signaling pathways in the vagina, draining lymph nodes and systemically. 2...
  62. Salpingeal infection model of Mycoplasma genitalium
    Patricia A Totten; Fiscal Year: 2010
    ..genitalium to cause disease and persist in the urethra, vagina, cervix, and oviducts of these animals. Although other primate species have been less extensively studied, M...
  63. Impact of suppressive antimicrobial therapy on the vaginal microbiota and BV
    RAYMOND SCOTT MCCLELLAND; Fiscal Year: 2013
    ..pyrosequencing to assess the impact of PPT on overall bacterial community composition and diversity in the vagina. Interventions that promote vaginal health by increasing the prevalence and quantity of Lactobacillus species and ..
  64. Comprehensive Evaluation of Prolapse Meshes by an Interdisciplinary Research Team
    Pamela A Moalli; Fiscal Year: 2013
    ..In the first phase of the study, we determine how biochemical and structural changes in the prolapsed vagina impact passive and active mechanical behavior so as to develop a mesh in which these deficiencies are repaired or ..
  65. ADOLESCENT MEDICINE HIV/AIDS RESEARCH NETWORK
    Anna Barbara Moscicki; Fiscal Year: 2001
    ..e. cervix, vagina, vulva,anus, penis, and scrotum) in adolescents infected with HIV and to compare the prevalence and severity of ..
  66. The Vaginal Microbiome: Disease, Genetics and the Environment
    Gregory Buck; Fiscal Year: 2009
    DESCRIPTION: The vagina is an interactive interface with the environment, and as such is covered by a protective epithelial surface...
  67. Mucus Penetrating Nanoparticles for Vaginal Drug Delivery
    Justin Hanes; Fiscal Year: 2009
    ..our mucus-penetrating, and cell-adherent, particles are retained more efficiently and for longer times in the vagina than conventional mucophilic nanoparticles...
  68. Mucus-penetrating nanoparticles for sustained microbicide delivery
    Justin Hanes; Fiscal Year: 2009
    ..particles (CP) are mucoadhesive and stick to the outer layers of mucus that are shed most rapidly out of the vagina. By densely coating MPP with low molecular weight polyethylene glycol (PEG) we found that unexpectedly large MPP ..
  69. INTRAVAGINAL PRODUCTS EFFECT ON VAGINAL PHYSIOLOGY
    David Eschenbach; Fiscal Year: 1999
    The vagina is the staging site for many genital infections. The long-term goal of this project is to understand how use of vaginal products could increase or decrease susceptibility to infections acquired during sexual intercourse...
  70. CHLORHEXIDINE IRRIGATION TO PREVENT INFECTION
    Dwight Rouse; Fiscal Year: 2001
    ..and endometritis is ascending infection of endogenous cervico-vaginal bacteria, intrapartum irrigation of the vagina and cervix with an anti- bacterial as a logical approach to prevention of peripartal infection...
  71. F.nucleatum in Amniotic Fluid Infection
    Yiping Han; Fiscal Year: 2004
    ..One hypothesis to explain this is that the organisms originate from the vagina and ascend into the uterus. However, some organisms from AF appear to be of oral origin...
  72. ACCEPTABILITY AND SAFETY STUDY OF JANESWAY FEMALE CONDOM
    Ron Frezieres; Fiscal Year: 2000
    There is a compelling public health interest in perfecting female condoms that line the vagina and protect the female genital tract against exposure to semen and potentially infected genital secretions...
  73. Structure/Function of Human Papillomavirus Oncoproteins
    Ronen Marmorstein; Fiscal Year: 2006
    ..HPV infections are also implicated in other malignancies including cancer of the vulva, vagina, penis, anus, skin, esophagus, and oropharyngeal region...
  74. PARTURITION INDUCED PELVIC FLOOR NEUROPATHY
    KARL THOR; Fiscal Year: 2003
    Pelvic organ prolapse (descent of the bladder, vagina, and rectum) and associated bladder and bowel dysfunction are problems for many women...
  75. Identification of Relaxin Receptor Antagonists
    Sheau Yu Hsu; Fiscal Year: 2005
    ..the induction of collagen remodeling and the consequent softening of the tissues of the birth canal (cervix and vagina), growth and differentiation of the mammary gland, and dilation of the blood vessels...