epidermis

Summary

Summary: The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).

Top Publications

  1. ncbi Wound healing--aiming for perfect skin regeneration
    P Martin
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 276:75-81. 1997
  2. ncbi p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
    A Yang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 398:714-8. 1999
  3. ncbi p63 is a p53 homologue required for limb and epidermal morphogenesis
    A A Mills
    Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 398:708-13. 1999
  4. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
  5. ncbi The cornified envelope: a model of cell death in the skin
    Eleonora Candi
    Biochemistry Laboratory, IDI IRCCS, c o Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
    Nat Rev Mol Cell Biol 6:328-40. 2005
  6. ncbi Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche
    Cedric Blanpain
    Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 118:635-48. 2004
  7. ncbi Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis
    Mayumi Ito
    Department of Dermatology, Kligman Laboratories, University of Pennsylvania School of Medicine, M8 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Nat Med 11:1351-4. 2005
  8. pmc p63 identifies keratinocyte stem cells
    G Pellegrini
    Laboratory of Tissue Engineering IDI, Istituto Dermopatico dell Immacolata, 00040 Rome, Italy
    Proc Natl Acad Sci U S A 98:3156-61. 2001
  9. ncbi De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin
    U Gat
    Howard Hughes Medical Institute, The University of Chicago, Illinois 60637, USA
    Cell 95:605-14. 1998
  10. pmc Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice
    Mikio Furuse
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 156:1099-111. 2002

Research Grants

Detail Information

Publications296 found, 100 shown here

  1. ncbi Wound healing--aiming for perfect skin regeneration
    P Martin
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Science 276:75-81. 1997
    ....
  2. ncbi p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
    A Yang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 398:714-8. 1999
    ..The embryonic epidermis of p63-/- mice undergoes an unusual process of non-regenerative differentiation, culminating in a striking ..
  3. ncbi p63 is a p53 homologue required for limb and epidermal morphogenesis
    A A Mills
    Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 398:708-13. 1999
    ..Thus, in contrast to p53, p63 is essential for several aspects of ectodermal differentiation during embryogenesis...
  4. pmc Defining the epithelial stem cell niche in skin
    Tudorita Tumbar
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, Rockefeller University, New York, NY 10021, USA
    Science 303:359-63. 2004
    ..Many of the >100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment...
  5. ncbi The cornified envelope: a model of cell death in the skin
    Eleonora Candi
    Biochemistry Laboratory, IDI IRCCS, c o Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
    Nat Rev Mol Cell Biol 6:328-40. 2005
    The epidermis functions as a barrier against the environment by means of several layers of terminally differentiated, dead keratinocytes - the cornified layer, which forms the endpoint of epidermal differentiation and death...
  6. ncbi Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche
    Cedric Blanpain
    Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 118:635-48. 2004
    ..the bulge), which can be mobilized to regenerate the new follicle with each hair cycle and to reepithelialize epidermis during wound repair...
  7. ncbi Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis
    Mayumi Ito
    Department of Dermatology, Kligman Laboratories, University of Pennsylvania School of Medicine, M8 Stellar Chance Building, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA
    Nat Med 11:1351-4. 2005
    ..We show that ablation leads to complete loss of hair follicles but survival of the epidermis. Through fate-mapping experiments, we find that stem cells in the hair follicle bulge do not normally contribute ..
  8. pmc p63 identifies keratinocyte stem cells
    G Pellegrini
    Laboratory of Tissue Engineering IDI, Istituto Dermopatico dell Immacolata, 00040 Rome, Italy
    Proc Natl Acad Sci U S A 98:3156-61. 2001
    ..The identification of p63 as a keratinocyte stem cell marker will be of practical importance for the clinical application of epithelial cultures in cell therapy as well as for studies on epithelial tumorigenesis...
  9. ncbi De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin
    U Gat
    Howard Hughes Medical Institute, The University of Chicago, Illinois 60637, USA
    Cell 95:605-14. 1998
    ..Our findings suggest that transient beta-catenin stabilization may be a key player in the long-sought epidermal signal leading to hair development and implicate aberrant beta-catenin activation in hair tumors...
  10. pmc Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice
    Mikio Furuse
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 156:1099-111. 2002
    ..been thought to play an important role in the barrier function of mammalian stratified epithelia, including the epidermis. Here we generated claudin-1-deficient mice and found that the animals died within 1 d of birth with wrinkled ..
  11. pmc Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis
    Kim B Jensen
    Laboratory for Epidermal Stem Cell Biology, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Cell Stem Cell 4:427-39. 2009
    ..We show that, in mouse epidermis, Lrig1 defines the hair follicle junctional zone adjacent to the sebaceous glands and infundibulum...
  12. ncbi The skin: an indispensable barrier
    Ehrhardt Proksch
    Department of Dermatology, University Hospitals of Schleswig Holstein, Kiel, Germany
    Exp Dermatol 17:1063-72. 2008
    ..The nucleated epidermis also contributes to the barrier through tight, gap and adherens junctions, as well as through desmosomes and ..
  13. pmc Asymmetric cell divisions promote Notch-dependent epidermal differentiation
    Scott E Williams
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Nature 470:353-8. 2011
    ....
  14. pmc Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein
    P J Koch
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Cell Biol 151:389-400. 2000
    ....
  15. pmc The magical touch: genome targeting in epidermal stem cells induced by tamoxifen application to mouse skin
    V Vasioukhin
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 96:8551-6. 1999
    ..This promoter is strongly active in dividing cells of epidermis and some other stratified squamous epithelia...
  16. ncbi WNT signals are required for the initiation of hair follicle development
    Thomas Andl
    Department of Dermatology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Cell 2:643-53. 2002
    ..This phenotype indicates that activation of WNT signaling in the skin precedes, and is required for, localized expression of regulatory genes and initiation of hair follicle placode formation...
  17. pmc Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila
    D P Kiehart
    Department of Cell Biology, Cell and Molecular Biology Program, University Program in Genetics and the Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
    J Cell Biol 149:471-90. 2000
    ..E., A.M. Richman, A.S. Ketchum, and D.P. Kiehart. 1993. Genes Dev. 7:29-41) that the leading edge of the lateral epidermis is a contractile purse-string that provides force for dorsal closure...
  18. ncbi Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila
    A Wodarz
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Federal Republic of Germany
    Cell 82:67-76. 1995
    ..Our results suggest that crumbs plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells of Drosophila...
  19. pmc p63 regulates proliferation and differentiation of developmentally mature keratinocytes
    Amy B Truong
    VA Palo Alto Health Care System, Palo Alto, California 94304, USA
    Genes Dev 20:3185-97. 2006
    ..context, we used siRNAs directed against p63 to down-regulate p63 expression in regenerating human epidermis. Loss of p63 resulted in severe tissue hypoplasia and inhibited both stratification and differentiation in a ..
  20. ncbi Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells
    Miriam Merad
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nat Rev Immunol 8:935-47. 2008
    ..new mouse models have led to the identification of other langerin(+) DC subsets that are not present in the epidermis, including a subset of DCs that is found in most non-lymphoid tissues...
  21. pmc p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis
    Michael Y Fessing
    Centre for Skin Sciences, University of Bradford, Bradford BD7 1DP, England, UK
    J Cell Biol 194:825-39. 2011
    ..region of the Satb1 gene, and p63 ablation results in marked reduction in the Satb1 expression levels in the epidermis. Satb1(-/-) mice show impaired epidermal morphology...
  22. pmc Epidermal homeostasis: a balancing act of stem cells in the skin
    Cedric Blanpain
    Universite Libre de Bruxelles, Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire, 808, Route de Lennik, 1070 Bruxelles, Belgium
    Nat Rev Mol Cell Biol 10:207-17. 2009
    The skin epidermis and its array of appendages undergo ongoing renewal by a process called homeostasis...
  23. pmc IL-23 stimulates epidermal hyperplasia via TNF and IL-20R2-dependent mechanisms with implications for psoriasis pathogenesis
    Jason R Chan
    Discovery Research, Schering Plough Biopharma Formerly DNAX Research, Inc, Palo Alto, CA 94304, USA
    J Exp Med 203:2577-87. 2006
    ..IL-23-dependent epidermal hyperplasia was observed in IL-19-/- and IL-24-/- mice, but was inhibited in IL-20R2-/- mice. These data implicate IL-23 in the pathogenesis of psoriasis and support IL-20R2 as a novel therapeutic target...
  24. pmc E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctions
    Judith A Tunggal
    Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
    EMBO J 24:1146-56. 2005
    ..To determine the role of E-cadherin in stratified epithelium of the epidermis, we have conditionally inactivated its gene in mice...
  25. ncbi Topographically distinct epidermal nociceptive circuits revealed by axonal tracers targeted to Mrgprd
    Mark J Zylka
    Division of Biology, 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Neuron 45:17-25. 2005
    ..Surprisingly, Mrgprd(+) innervation is restricted to the epidermis and absent from specialized sensory structures...
  26. pmc Deconstructing the skin: cytoarchitectural determinants of epidermal morphogenesis
    Cory L Simpson
    Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611, USA
    Nat Rev Mol Cell Biol 12:565-80. 2011
    To provide a stable environmental barrier, the epidermis requires an integrated network of cytoskeletal elements and cellular junctions...
  27. ncbi Klf4 is a transcription factor required for establishing the barrier function of the skin
    J A Segre
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Illinois 60637, USA
    Nat Genet 22:356-60. 1999
    Located at the interface between body and environment, the epidermis must protect the body against toxic agents and dehydration, and protect itself against physical and mechanical stresses...
  28. pmc Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells
    Elena Ezhkova
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA
    Cell 136:1122-35. 2009
    ..They maintain their proliferative potential and globally repressing undesirable differentiation programs while selectively establishing a specific terminal differentiation program in a stepwise fashion...
  29. ncbi Zebrafish early macrophages colonize cephalic mesenchyme and developing brain, retina, and epidermis through a M-CSF receptor-dependent invasive process
    P Herbomel
    Unité de Génétique des Déficits Sensoriels, URA 1968 du CNRS, Departement des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, Paris Cedex 15, 75724, France
    Dev Biol 238:274-88. 2001
    ..that these early macrophages spread in the whole cephalic mesenchyme, and from there invade epithelial tissues: epidermis, retina, and brain--especially the optic tectum...
  30. pmc Transdifferentiation of corneal epithelium into epidermis occurs by means of a multistep process triggered by dermal developmental signals
    David J Pearton
    Equipe Biologie de la Différenciation Epithéliale, UMR CNRS 5538, Laboratoire d Etude de la Différentiation et de l Adhérence Cellulaire, Institut Albert Bonniot, Universite Joseph Fourier, BP 53 38041 Grenoble cedex 9, France
    Proc Natl Acad Sci U S A 102:3714-9. 2005
    ..of the corneal epithelium are converted to hair, along with their associated stem cells, then interfollicular epidermis, by means of a multistep process triggered by dermal developmental signals...
  31. pmc Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells in skin
    B J Merrill
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
    Genes Dev 15:1688-705. 2001
    In skin, multipotent stem cells generate the keratinocytes of the epidermis, sebaceous gland, and hair follicles. In this paper, we show that Tcf3 and Lef1 control these differentiation lineages...
  32. pmc Role of integrins in regulating epidermal adhesion, growth and differentiation
    Fiona M Watt
    Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 21:3919-26. 2002
    Mammalian epidermis is renewed throughout life by proliferation of a multipotential stem cell population and terminal differentiation of stem cell progeny...
  33. pmc Epidermal stem cell diversity and quiescence
    Fiona M Watt
    Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge, UK
    EMBO Mol Med 1:260-7. 2009
    Mammalian epidermis is maintained by self-renewal of stem cells and terminal differentiation of their progeny. New data reveal a diversity amongst stem cells that was previously unrecognized...
  34. pmc Genome-wide expression profiling of five mouse models identifies similarities and differences with human psoriasis
    William R Swindell
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e18266. 2011
    ....
  35. pmc Dual role of COUP-TF-interacting protein 2 in epidermal homeostasis and permeability barrier formation
    Olga Golonzhka
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    J Invest Dermatol 129:1459-70. 2009
    ..CTIP2 is also highly expressed in the developing epidermis, and at lower levels in the dermis and in adult skin...
  36. ncbi Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis
    James Legg
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Development 130:6049-63. 2003
    Human interfollicular epidermis is renewed by stem cells that are clustered in the basal layer in a patterned, non-random distribution...
  37. pmc EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair
    Elena Ezhkova
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Genes Dev 25:485-98. 2011
    ..hair follicles (HFs) arrest morphogenesis and degenerate due to defective proliferation and increased apoptosis, epidermis hyperproliferates and survives engraftment...
  38. ncbi Unique and redundant functions of integrins in the epidermis
    Coert Margadant
    Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    FASEB J 24:4133-52. 2010
    ..The epithelium of the skin, the epidermis, is in a continuous equilibrium of growth and differentiation and has the remarkable capacity to self-renew ..
  39. ncbi Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis
    Kristin M Braun
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 130:5241-55. 2003
    Mammalian epidermis is maintained by stem cells that have the ability to self-renew and generate daughter cells that differentiate along the lineages of the hair follicles, interfollicular epidermis and sebaceous gland...
  40. ncbi Transient activation of beta-catenin signalling in adult mouse epidermis is sufficient to induce new hair follicles but continuous activation is required to maintain hair follicle tumours
    Cristina Lo Celso
    Keratinocyte Laboratory, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Development 131:1787-99. 2004
    ..signalling is disturbed from mid-gestation onwards lineage commitment is profoundly altered in postnatal mouse epidermis. We have investigated whether adult epidermis has the capacity for beta-catenin-induced lineage conversion ..
  41. pmc Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation
    T M Svitkina
    Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Cell Biol 139:397-415. 1997
    ..We propose that the forward translocation of the cell body and retrograde flow in the lamellipodia are both driven by contraction of an actin-myosin network in the lamellipodial/cell body transition zone...
  42. ncbi A skin microRNA promotes differentiation by repressing 'stemness'
    Rui Yi
    Howard Hughes Medical Institute, The Rockefeller University, New York City, New York 10065, USA
    Nature 452:225-9. 2008
    ..Our findings suggest that miR-203 defines a molecular boundary between proliferative basal progenitors and terminally differentiating suprabasal cells, ensuring proper identity of neighbouring layers...
  43. ncbi Epidermal viral immunity induced by CD8alpha+ dendritic cells but not by Langerhans cells
    Rhys S Allan
    Department of Microbiology and Immunology, The University of Melbourne, Melbourne, Victoria 3010, Australia
    Science 301:1925-8. 2003
    ..paradigm for dendritic cell function derives from the study of Langerhans cells, which predominate within skin epidermis. After an encounter with foreign agents, Langerhans cells are thought to migrate to draining lymph nodes, where ..
  44. pmc Cellular and genetic analysis of wound healing in Drosophila larvae
    Michael J Galko
    Howard Hughes Medical Institute and Department of BiochemistryStanford University School of Medicine, Stanford, California, USA
    PLoS Biol 2:E239. 2004
    ..Cell biological and molecular parallels to vertebrate wound healing lead us to speculate that wound healing is an ancient response that has diversified during evolution...
  45. ncbi Barrier function of the skin: "la raison d'être" of the epidermis
    Kathi C Madison
    Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa, Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa, USA
    J Invest Dermatol 121:231-41. 2003
    The primary function of the epidermis is to produce the protective, semi-permeable stratum corneum that permits terrestrial life...
  46. ncbi TGF-beta is required to maintain the pool of immature Langerhans cells in the epidermis
    Junda M Kel
    Department of Immunology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands
    J Immunol 185:3248-55. 2010
    ..While initial LC seeding occurred in DC-TbetaR1(del) mice, the cells disappeared from the epidermis during the first week of life...
  47. pmc Robust control of mitotic spindle orientation in the developing epidermis
    Nicholas D Poulson
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 191:915-22. 2010
    ..In the epidermis, progenitors divide symmetrically to increase surface area and asymmetrically to promote stratification...
  48. pmc Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis
    Ryan R Driskell
    Wellcome Trust Centre for Stem Cell Research, Cambridge, UK
    Development 136:2815-23. 2009
    ..We have thus demonstrated a previously unrecognised heterogeneity in dermal papilla cells and shown that Sox2-positive cells specify particular hair follicle types...
  49. pmc Neuroendocrine activity of the melanocyte
    Andrzej Slominski
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, TN, USA
    Exp Dermatol 18:760-3. 2009
    ..Such capabilities have defined melanocytes as neuroendocrine cells that not only coordinate cutaneous but also can affect a global homeostasis...
  50. pmc Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3
    George L Sen
    VA Palo Alto Health Care System, Palo Alto, California 94305, USA
    Genes Dev 22:1865-70. 2008
    ..In the epidermis, a tissue that balances stem cell self-renewal with differentiation, H3K27me3, occupies the promoters of many ..
  51. ncbi Epithelial stem cells: a folliculocentric view
    George Cotsarelis
    Department of Dermatology, Kligman Laboratories, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Invest Dermatol 126:1459-68. 2006
    ..Bulge cells only contribute to the epidermis during wound healing, but after isolation, when combined with neonatal dermal cells, they regenerate new hair ..
  52. pmc Jarid2 regulates mouse epidermal stem cell activation and differentiation
    Stefania Mejetta
    Department of Differentiation and Cancer, Center for Genomic Regulation and UPF, Barcelona, Spain
    EMBO J 30:3635-46. 2011
    ..Here, we show that deletion of Jarid2 in mouse epidermis reduces the proliferation and potentiates the differentiation of postnatal epidermal progenitors, without ..
  53. pmc The human keratins: biology and pathology
    Roland Moll
    Institute of Pathology, Philipps University, 35033, Marburg, Germany
    Histochem Cell Biol 129:705-33. 2008
    ..Future research might open further fields of clinical application for this remarkable protein family...
  54. ncbi Regulation of cutaneous malignancy by gammadelta T cells
    M Girardi
    Department of Dermatology and Yale Skin Diseases Research Core Center, King s College, London SE1 9RT, UK
    Science 294:605-9. 2001
    ..Thus, local T cells may use evolutionarily conserved proteins to negatively regulate malignancy...
  55. pmc Skin and hair follicle integrity is crucially dependent on beta 1 integrin expression on keratinocytes
    C Brakebusch
    Department of Experimental Pathology, Lund University, S 221 85 Lund, Sweden
    EMBO J 19:3990-4003. 2000
    ..Eventually, the malformed hair follicles were removed by infiltrating macrophages. The epidermis of the back skin became hyperthickened, the basal keratinocytes showed reduced expression of alpha 6 beta 4 ..
  56. ncbi Sonic hedgehog signaling is essential for hair development
    B St-Jacques
    Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Curr Biol 8:1058-68. 1998
    ..and bone morphogenetic protein families (Wnt10b, Sonic hedgehog (Shh) and Bmp2/Bmp4, respectively) in the epidermis correlates with the initiation of hair follicle formation...
  57. pmc Asymmetric cell divisions promote stratification and differentiation of mammalian skin
    Terry Lechler
    Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA
    Nature 437:275-80. 2005
    The epidermis is a stratified squamous epithelium forming the barrier that excludes harmful microbes and retains body fluids...
  58. pmc Activation of TLR2 by a small molecule produced by Staphylococcus epidermidis increases antimicrobial defense against bacterial skin infections
    Yuping Lai
    Division of Dermatology, Department of Medicine, University of California, San Diego and VA San Diego Healthcare System, San Diego, California 92161, USA
    J Invest Dermatol 130:2211-21. 2010
    ..epidermidis to activate TLR2 signaling and induce antimicrobial peptide expression, thus enabling the skin to mount an enhanced response to pathogens...
  59. ncbi Keratinocyte growth factor stimulates migration and hyaluronan synthesis in the epidermis by activation of keratinocyte hyaluronan synthases 2 and 3
    Susanna Karvinen
    Department of Anatomy, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland
    J Biol Chem 278:49495-504. 2003
    ..Hyaluronan, an extracellular matrix glycosaminoglycan that accumulates in wounded epidermis, is known to promote cell migration, suggesting that increased synthesis of hyaluronan might be associated with ..
  60. ncbi The skin barrier as an innate immune element
    Peter M Elias
    Department of Dermatology, University of California San Francisco Medical Center, San Francisco, CA, USA
    Semin Immunopathol 29:3-14. 2007
    ..Yet, the epidermis mediates a broad set of protective 'barrier' functions that includes defense against pathogen challenges...
  61. pmc Epidermal stem cells are defined by global histone modifications that are altered by Myc-induced differentiation
    Michaela Frye
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 2:e763. 2007
    ..of Myc induces epidermal stem cells to exit their niche and differentiate into sebocytes and interfollicular epidermis, a process that is associated with widespread changes in gene transcription...
  62. ncbi Biochemical, structural, and transglutaminase substrate properties of human loricrin, the major epidermal cornified cell envelope protein
    E Candi
    Skin Biology Branch, NIAMS, National Institutes of Health, Bethesda, Maryland 20892 2775, USA
    J Biol Chem 270:26382-90. 1995
    ..We have also isolated loricrin from newborn mouse epidermis. By circular dichroism and fluorescence spectroscopy, the in vivo mouse and bacterially expressed human ..
  63. ncbi The emerging roles of serine protease cascades in the epidermis
    Petra Ovaere
    Department of Biomedical Molecular Biology, Ghent University, Belgium
    Trends Biochem Sci 34:453-63. 2009
    ..Therefore, a deeper knowledge of the regulation of these serine protease cascades could form the basis for development of appropriate treatments for skin disorders such as Netherton syndrome...
  64. ncbi Degradation of corneodesmosome proteins by two serine proteases of the kallikrein family, SCTE/KLK5/hK5 and SCCE/KLK7/hK7
    Cecile Caubet
    UMR5165 Epidermis Differentiation and Rheumatoid Autoimmunity, CNRS P Sabatier University Institut Fédératif de Recherche 30 and INSERM CNRS Université P Sabatier Centre Hospitalier Universitaire, Toulouse, France
    J Invest Dermatol 122:1235-44. 2004
    ..The degradation of these proteins at the epidermis surface is necessary for desquamation...
  65. pmc Conditional ablation of beta1 integrin in skin. Severe defects in epidermal proliferation, basement membrane formation, and hair follicle invagination
    S Raghavan
    Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
    J Cell Biol 150:1149-60. 2000
    ..This, in turn, is required for hemidesmosome stability, epidermal proliferation, and hair follicle morphogenesis. However, beta1 downregulation does not provide the trigger to terminally differentiate...
  66. ncbi Identification of the cell lineage at the origin of basal cell carcinoma
    Khalil Kass Youssef
    Universite Libre de Bruxelles ULB, IRIBHM, Brussels B 1070, Belgium
    Nat Cell Biol 12:299-305. 2010
    ..Smoothened mutant (SmoM2), we activated Hedgehog signalling in different cellular compartments of the skin epidermis and determined in which compartments Hedgehog activation induces BCC formation...
  67. ncbi New perspectives on epidermal barrier dysfunction in atopic dermatitis: gene-environment interactions
    Michael J Cork
    Pediatric Dermatology Clinic, Children s Hospital, Sheffield S10 2RX, UK
    J Allergy Clin Immunol 118:3-21; quiz 22-3. 2006
    ..One or more of these factors in combination might lead to a defective barrier, thereby increasing the risk of allergen penetration and succeeding inflammatory reaction, thus contributing to exacerbations of this disease...
  68. pmc Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment
    Shadmehr Demehri
    Department of Developmental Biology, Division of Dermatology, Washington University School of Medicine, Saint Louis, MO 63110 1095, USA
    Cancer Cell 16:55-66. 2009
    ..paralogues that impaired the skin barrier also predisposed Notch1-expressing skin to tumorigenesis, demonstrating that the tumor-promoting effect of Notch1 loss involves a crosstalk between barrier-defective epidermis and its stroma.
  69. ncbi IL-22 and IL-20 are key mediators of the epidermal alterations in psoriasis while IL-17 and IFN-gamma are not
    Kerstin Wolk
    Interdisciplinary Group of Molecular Immunopathology, Dermatology Medical Immunology, University Hospital Charite, Chariteplatz 1, 10117, Berlin, Germany
    J Mol Med (Berl) 87:523-36. 2009
    ..IL-22 and, with lower potency, IL-20 caused psoriasis-like morphological changes in a three-dimensional human epidermis model...
  70. ncbi Absence of integrin alpha 6 leads to epidermolysis bullosa and neonatal death in mice
    E Georges-Labouesse
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Illkirch, C U de Strasbourg, France
    Nat Genet 13:370-3. 1996
    ..Our study provides evidence that some forms of epidermolysis bullosa may originate from defects of the alpha 6 gene...
  71. ncbi Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin
    Hugo J Snippert
    Hubrecht Institute KNAW Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, Netherlands
    Science 327:1385-9. 2010
    Mammalian epidermis consists of three self-renewing compartments: the hair follicle, the sebaceous gland, and the interfollicular epidermis. We generated knock-in alleles of murine Lgr6, a close relative of the Lgr5 stem cell gene...
  72. pmc Epidermal Notch signalling: differentiation, cancer and adhesion
    Fiona M Watt
    Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Curr Opin Cell Biol 20:171-9. 2008
    ..The effects on differentiation can be cell autonomous and non-autonomous, and Notch contributes to stem cell clustering via modulation of cell adhesion...
  73. ncbi Distinct contribution of stem and progenitor cells to epidermal maintenance
    Guilhem Mascre
    Universite Libre de Bruxelles, IRIBHM, Brussels B 1070, Belgium
    Nature 489:257-62. 2012
    The skin interfollicular epidermis (IFE) is the first barrier against the external environment and its maintenance is critical for survival. Two seemingly opposite theories have been proposed to explain IFE homeostasis...
  74. ncbi A homolog of Drosophila grainy head is essential for epidermal integrity in mice
    Stephen B Ting
    Rotary Bone Marrow Research Laboratories, c o Royal Melbourne Hospital Post Office, Grattan Street, Parkville, Victoria, Australia 3050
    Science 308:411-3. 2005
    ..of transglutaminase 1, the key enzyme involved in cross-linking the structural components of the superficial epidermis. These findings suggest that the functional mechanisms involving protein cross-linking that maintain the ..
  75. pmc HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells
    Christopher Schuster
    Department of Dermatology, DIAID, 1090 Vienna, Austria
    J Exp Med 206:169-81. 2009
    ..We also found that CD45(+)HLA-DR(high)CD1c(+) dendritic cells (DCs) are already present in the epidermis and dermis at 9 wk estimated gestational age (EGA) and that transforming growth factor beta1 production precedes ..
  76. ncbi Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: evidence for a role in pain
    Peng Zhao
    Department of Neurology and Center for Neuroscience and Regeneration Research, Yale University School of Medicine, New Haven, CT 06510, USA
    Pain 139:90-105. 2008
    ..Na(v)1.1, Na(v)1.6, and Na(v)1.8 were localized within keratinocytes in rat epidermis. In addition, sodium channels contribute to the release of ATP from rat keratinocytes in response to increased [..
  77. ncbi p63 Is essential for the proliferative potential of stem cells in stratified epithelia
    Makoto Senoo
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:523-36. 2007
    ..Together, these data identify p63 as a key, lineage-specific determinant of the proliferative capacity in stem cells of stratified epithelia...
  78. ncbi The epidermis as an adjuvant
    Danuta Gutowska-Owsiak
    MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
    J Invest Dermatol 132:940-8. 2012
    It is now clear that the epidermis has an active role in local immune responses in the skin...
  79. pmc E6 and E7 from beta HPV38 cooperate with ultraviolet light in the development of actinic keratosis-like lesions and squamous cell carcinoma in mice
    Daniele Viarisio
    DKFZ, Heidelberg, Germany
    PLoS Pathog 7:e1002125. 2011
    ..Viral oncogene expression led to increased cellular proliferation in the epidermis of the Tg animals in comparison to the wild-type littermates...
  80. pmc External antigen uptake by Langerhans cells with reorganization of epidermal tight junction barriers
    Akiharu Kubo
    Department of Dermatology, School of Medicine, Keio University, Tokyo 160 8582, Japan
    J Exp Med 206:2937-46. 2009
    ....
  81. ncbi Keratinocyte dysfunction in vitiligo epidermis: cytokine microenvironment and correlation to keratinocyte apoptosis
    Silvia Moretti
    Department of Dermatological Sciences, University of Florence, Florence, Italy
    Histol Histopathol 24:849-57. 2009
    ..melanogenic mediators and the level of functioning keratinocytes may affect melanocyte dysfunction in vitiligo epidermis. Keratinocyte-derived mediators involved in pigmentation, analysed by in situ hybridization, and epidermal ..
  82. ncbi Assaying proliferation and differentiation capacity of stem cells using disaggregated adult mouse epidermis
    Kim B Jensen
    Laboratory for Epidermal Stem Cell Biology, Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge, UK
    Nat Protoc 5:898-911. 2010
    In this protocol, we describe how to isolate keratinocytes from adult mouse epidermis, fractionate them into different sub-populations on the basis of cell surface markers and examine their function in an in vivo skin reconstitution ..
  83. ncbi Transglutaminases in skin epidermis
    Kiyotaka Hitomi
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464 8601, Japan
    Eur J Dermatol 15:313-9. 2005
    ..The cornified cell envelope (CE) is a critical structure for barrier function at the outermost layer of the skin epidermis. For CE formation in terminal keratinocyte differentiation, covalent cross-linking of constituting proteins such ..
  84. ncbi Tiling of the Drosophila epidermis by multidendritic sensory neurons
    Wesley B Grueber
    Howard Hughes Medical Institute, Department of Physiology, University of California, San Francisco, San Francisco, CA 94143 0725, USA
    Development 129:2867-78. 2002
    ..We show that each of the 15 neurons per abdominal hemisegment spread dendrites to characteristic regions of the epidermis. We place these neurons into four distinct morphological classes distinguished primarily by their dendrite ..
  85. ncbi The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds
    Wei Li
    Department of Protein Engineering, Genentech, Inc, South San Francisco, California 94080, USA
    J Biol Chem 284:218-28. 2009
    ..In the keratinizing stratified squamous epidermis of skin, however, its expression was induced only during epidermal hyperproliferation, such as in psoriasis and ..
  86. pmc Hdac1 and Hdac2 act redundantly to control p63 and p53 functions in epidermal progenitor cells
    Matthew LeBoeuf
    Department of Dermatology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
    Dev Cell 19:807-18. 2010
    ..Our data identify critical requirements for HDAC1/2 in epidermal development and indicate that HDAC1/2 directly mediate repressive functions of p63 and suppress p53 activity...
  87. ncbi Interleukin 20: discovery, receptor identification, and role in epidermal function
    H Blumberg
    Department of Genetics, ZymoGenetics, Inc, 1201 Eastlake Avenue E, Seattle, WA 98102, USA
    Cell 104:9-19. 2001
    ..Taken together, these results demonstrate a role in epidermal function and psoriasis for IL-20, a novel cytokine identified solely by bioinformatics analysis...
  88. pmc Autocrine/paracrine TGFbeta1 is required for the development of epidermal Langerhans cells
    Daniel H Kaplan
    Department of Dermatology, 2Department of Laboratory Medicine, 3Section of Immunobiology, and 4Section of Medical Oncology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 204:2545-52. 2007
    ..Thus, TGFbeta1 derived from LCs acts directly on LCs through an autocrine/paracrine loop, and it is required for LC development and/or survival...
  89. pmc DNMT1 maintains progenitor function in self-renewing somatic tissue
    George L Sen
    Programs in Epithelial Biology and Cancer Biology and the Stanford Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California 94305, USA
    Nature 463:563-7. 2010
    ..role for DNMT1 in maintaining the progenitor state in constantly replenished somatic tissues, such as mammalian epidermis, is unclear. Here we show that DNMT1 is essential for epidermal progenitor cell function...
  90. pmc Mice deficient in involucrin, envoplakin, and periplakin have a defective epidermal barrier
    Lisa M Sevilla
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, England, UK
    J Cell Biol 179:1599-612. 2007
    ..Thus, combined loss of the cornified envelope proteins not only impairs the epidermal barrier, but also changes the composition of T cell subpopulations in the skin...
  91. ncbi Desmoplakin is essential in epidermal sheet formation
    V Vasioukhin
    Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois 60637, USA
    Nat Cell Biol 3:1076-85. 2001
    We have generated an epidermis-specific desmoplakin (DP) mouse knockout, and show that epidermal integrity requires DP; mechanical stresses to DP-null skin cause intercellular separations...
  92. ncbi An epidermal barrier wound repair pathway in Drosophila is mediated by grainy head
    Kimberly A Mace
    Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Science 308:381-5. 2005
    ..The conservation of this Grh-dependent pathway suggests that the repair of insect cuticle and mammal skin is controlled by an ancient, shared control system for constructing and healing the animal body surface barrier...
  93. ncbi S100 proteins in the epidermis
    Richard L Eckert
    Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Invest Dermatol 123:23-33. 2004
    ..S100A8, S100A9, S100A10, S100A11, S100A12, S100A15, S100B, and S100P) are expressed in normal and/or diseased epidermis. S100 proteins exist in cells as anti-parallel hetero- and homodimers and upon calcium binding interact with ..
  94. pmc Fibroblast growth factor receptors 1 and 2 in keratinocytes control the epidermal barrier and cutaneous homeostasis
    Jingxuan Yang
    Department of Biology, Institute of Cell Biology, Eidgenossische Technische Hochschule Zurich, 8093 Zurich, Switzerland
    J Cell Biol 188:935-52. 2010
    ..Our results identify essential roles for FGFs in the regulation of the epidermal barrier and in the prevention of cutaneous inflammation, and highlight the importance of stromal-epithelial interactions in skin homeostasis and disease...
  95. ncbi Skin barrier function, epidermal proliferation and differentiation in eczema
    Ehrhardt Proksch
    Department of Dermatology, University of Kiel, Schittenhelmstr 7, 24105 Kiel, Germany
    J Dermatol Sci 43:159-69. 2006
    ..Common treatment strategies for eczema include the application of lipid-based creams and ointments, which aim toward the restoration of the defective permeability barrier, thus helping to normalize proliferation and differentiation...
  96. pmc Identification of a novel population of Langerin+ dendritic cells
    Laura S Bursch
    Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA
    J Exp Med 204:3147-56. 2007
    Langerhans cells (LCs) are antigen-presenting cells that reside in the epidermis of the skin and traffic to lymph nodes (LNs)...
  97. pmc Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles
    Danelle Devenport
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA
    Nat Cell Biol 10:1257-68. 2008
    ..Finally, we provide in vitro evidence that homotypic intracellular interactions of Celsr1 are required to recruit Vangl2 and Fzd6 to sites of cell-cell contact...
  98. pmc Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera
    Qiang Fu
    School of Life Sciences, The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong University, Jinan, 250100, PR China
    BMC Genomics 10:600. 2009
    Metamorphosis is an important process in the life cycle of holometabolous insects and is regulated by insect hormones. During metamorphosis, the epidermis goes through a significant transformation at the biochemical and molecular levels.
  99. pmc Activators of PPARs and LXR decrease the adverse effects of exogenous glucocorticoids on the epidermis
    Marianne Demerjian
    Department of Dermatology, UCSF and VA Medical Center, San Francisco, CA 94121, USA
    Exp Dermatol 18:643-9. 2009
    While glucocorticoids (GC) exert beneficial effects (anti-inflammatory), they also have adverse effects on the epidermis including decreased epidermal differentiation, decreased keratinocyte proliferation, and decreased cutaneous ..
  100. ncbi Epidermal abnormalities and increased malignancy of skin tumors in human epidermal keratin 8-expressing transgenic mice
    M Llanos Casanova
    Epithelial Damage, Repair and Tissue Engineering, CIEMAT, Avenida Complutense 22, 28040 Madrid, Spain
    FASEB J 18:1556-8. 2004
    ..Transgenic mice expressing human K8 in the epidermis showed severe epidermal and hair follicle dysplasia with concomitant alteration in epidermal differentiation ..
  101. pmc More than one way to skin . .
    Elaine Fuchs
    Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA
    Genes Dev 22:976-85. 2008
    ..by epithelial-mesenchymal interactions to differentially adopt the cell fates that enable them to form the epidermis, hair follicle, and sebaceous gland...

Research Grants73

  1. Role and Regulation of INrf2
    ANIL KUMAR JAISWAL; Fiscal Year: 2013
    ..will continue with generation of INrf2 conditional knockout mice that do not express INrf2 protein in liver and epidermis of skin. The Cre-Lox system will be used to generate these mice...
  2. Nanopolymersomes for Transcutaneous Co-Delivery of Vaccine Antigens and Adjuvants
    PAUL CAMILLE TUMEH; Fiscal Year: 2013
    ..poly(ethylene oxide) (PEO) and polycaprolactone (PCL), will penetrate the stratum corneum to reach the vital epidermis and induce an antigen specific immune response...
  3. Prevention of UV-induced carcinogenesis by cyanidin-3-glucoside
    Xianglin Shi; Fiscal Year: 2013
    ..nick end labeling, and apoptotic cells together with a change in p53 and p21/cip1- positive cell population in epidermis. These studies will provide a mechanistic rationale for an early on C3G efficacy in skin cancer prevention...
  4. Targeting p53-Dependent Repigmentation in Vitiligo
    TAMARA G TERZIAN; Fiscal Year: 2013
    ..in a hair follicle niche can produce differentiated melanocytes that will repopulate the interfollicular epidermis and restore normal pigmentation. To accomplish the proposed aims, Dr...
  5. Epithelial differentiation in cornea
    Bogi Andersen; Fiscal Year: 2012
    ..After a period of epithelial thinning, the corneal epithelium undergoes differentiation to an epidermis-like structure...
  6. Somatic Stem Cells in Engineered Human Skin
    Steven Boyce; Fiscal Year: 2009
    ..and elective surgery and adnexal structures will be enabled by selection for the somatic stem cells (SSC) of the epidermis, and preservation of the SSC phenotype during keratinocyte culture;and, b) keratinocytes in CSS can be induced ..
  7. Testing the Therapeutic Potential of iPS Cells for Inherited Skin Diseases
    Jakub Tolar; Fiscal Year: 2013
    ..Since the epidermis is continuously renewed by stem cells in the proliferative basal layer, a permanent corrective therapy for EB ..
  8. A Cis-regulatory Model for Neural Border Induction
    Daniel Meulemans Medeiros; Fiscal Year: 2013
    ..population of multipotent progenitor cells that forms at the border of the developing central nervous system and epidermis. During early development, neural crest cells migrate from this neural border domain to form diverse derivatives ..
  9. Regulation/Role of AcylCer in Normal Epidermis and Atopic Dermatitis
    Peter M Elias; Fiscal Year: 2013
    ..to the w-hydroxy terminal of amide-linked very long-chain fatty acids (>C28), is not only unique to the epidermis, but also critical for normal permeability barrier homeostasis...
  10. Circadian Clock Regulation in Skin
    Bogi Andersen; Fiscal Year: 2013
    ..this central hypothesis: The circadian clock coordinates the timing of metabolism and cell proliferation in the epidermis, imposing separation between the times of maximum ROS generation and S-phase...
  11. Skin Regeneration with Stem Cells and Scaffolds
    SUSAN RENEE OPALENIK; Fiscal Year: 2013
    ..by scarring results from the faulty, exuberant reconstruction of the dermal architecture and the formation of an epidermis lacking appendages...
  12. Plexin signalling in melanocyte biology and melanoma progression
    Glynis A Scott; Fiscal Year: 2013
    ..While they are a minority of the population of the epidermis, they are responsible for the majority of photoprotection of the skin from ultraviolet radiation (UVR) through ..
  13. The consequences of loricrin deficiency on epidermal barrier function
    DENNIS ROOP; Fiscal Year: 2013
    ..Additionally, treated LKO mice developed an acanthotic epidermis with hyperkeratotic foci...
  14. Delivery of biologically active nucleic acids to epidermal cells
    ROGER L contact KASPAR; Fiscal Year: 2010
    ..that can be used in a validated and transferable algorithm for testing delivery of nucleic acids to cells in the epidermis. Because our models are activated by successful delivery, changes at the single cell level will be detectable...
  15. 3D human skin models
    Meenhard Herlyn; Fiscal Year: 2010
    ..The murine epidermis is very thin and largely devoid of melanocytes, whereas human epidermis is thick and contains pigment-producing ..
  16. Protein Therapy for Recessive Dystrophic Epidermolysis Bullosa
    David T Woodley; Fiscal Year: 2010
    ..the human gene encoding type VII collagen (C7), the major component of anchoring fibrils (AFs) that anchor the epidermis to the dermis. AFs are attenuated, diminutive, or absent in RDEB...
  17. Role of exogenous melatonin in skin biology
    Andrzej T Slominski; Fiscal Year: 2013
    ..Therefore, we are proposing: 1. To define the protective role of melatonin in human epidermis using epidermal keratinocytes and melanocytes cultured in vitro and human skin explants histocultured ex-vivo;2...
  18. Autophagy in epidermal melanocyte: a protective or a destructive role?
    Vijayasaradhi Setaluri; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Melanin in the skin protects the epidermis and the underlying dermis from the deleterious effects of ultraviolet radiation in the sun light...
  19. Antigen presentation by epithelial stem cells to promote life long immunity
    MARIE CLAIRE ELISABETH GAUDUIN; Fiscal Year: 2013
    ..promoter has extensively been studied and is restricted to terminally differentiated epithelial cells in epidermis and mucosa...
  20. The Molecular Genetics of Early Neurogenesis
    Ethan Bier; Fiscal Year: 2013
    ..In Drosophila and vertebrates alike, dorsal cells of the CNS form along the border of the BMP producing epidermis and express the Msx1 transcription factor (msh in flies), while other transcription factors Pax6 and Gsh (ind in ..
  21. Calcium-sensing Receptor and Keratinocyte Differentiation
    Chia Ling Tu; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Epidermis consists of multiple layers of keratinocytes, which differentiate and produce a permeability barrier that provides protection against environmental insults...
  22. Identification and Characterization of Adult Salivary Gland Stem Cells
    Soosan Ghazizadeh; Fiscal Year: 2013
    ..first to develop a method for in situ lineage tracing that we used to demonstrate the presence of stem cells in epidermis and sebaceous glands...
  23. Stat3 in Epitherlial Carcinogenesis
    John DiGiovanni; Fiscal Year: 2012
    ..During the previous funding period, through use of epidermis specific Stat3 knockout mice we discovered that Stat3 was required for de novo skin tumor development during two-..
  24. Role of Protein Kinase D in Skin Epithelia
    Soosan Ghazizadeh; Fiscal Year: 2012
    ..conditional PKD1 knockout mice to determine the contribution of PKD1 to the hyperplastic/adaptive responses of epidermis. Defining a critical role for PKD enzymes in epidermal adaptive responses will provide an opportunity to develop ..
  25. Role of vitamin D receptor in DNA repair
    Dennis H Oh; Fiscal Year: 2013
    ..In support of the hypothesis, we have observed that VDR is a UV- inducible gene, and that VDR knockout mouse epidermis is significantly impaired in the repair of the major UV photoproducts...
  26. Cellular Determinants of Ah Receptor Signaling
    Thomas R Sutter; Fiscal Year: 2013
    ..We propose to test the hypothesis that in the epidermis, TCDD-activated AHR disrupts the epidermal barrier by increasing the transcription of genes involved in the ..
  27. Characterization of Enhancers in the Epidermal Differentiation Complex
    CRISTINA DE GUZMAN STRONG; Fiscal Year: 2012
    ..6Mb and contains a majority of genes that are specifically expressed upon terminal differentiation of the epidermis and subsequent skin barrier formation...
  28. Apigenin restores TSP-1 expression in UVB-irradiated keratinocytes
    Jill C Pelling; Fiscal Year: 2013
    ..first protein inhibitor of angiogenesis to be identified (2), and its expression is markedly downregulated in epidermis following UVB irradiation and throughout distinct steps of skin carcinogenesis (3-6)...
  29. Akt/mTOR Signaling in Energy Balance Modulation of Epithelial Carcinogenesis
    Stephen D Hursting; Fiscal Year: 2013
    ..of the IGF-1 receptor (IGF-1R) and epidermal growth factor (EGF)-R, in several tissues including the epidermis;and ii) liver-specific IGF-1 deficiency (LID, resulting in reduced circulating IGF-1 levels) mimics CR by ..
  30. Stomate-based innate immunity against bacterial infection in Arabidopsis
    Maeli Melotto; Fiscal Year: 2012
    ..Recent studies provide evidence that plant stomata, pores in the epidermis of plants, function in innate immunity against the entry of bacteria into the plant...
  31. The Role of Protein Kinase D in Epidermal Tumorigenesis
    Wendy B Bollag; Fiscal Year: 2013
    ..BCCs, which make up approximately 80% of the NMSCs, arise from basal keratinocytes in the epidermis of the skin...
  32. Epidermal stem cell properties in mice with altered polyamines
    Lisa M Shantz; Fiscal Year: 2010
    Although it is known that the mammalian epidermis is maintained by self-renewal of stem cells, the underlying mechanisms, and the changes that occur in skin cancer, are not well-defined...
  33. Invasive Behavior of Tumor Cells Producing Collagenase-1
    Constance E Brinckerhoff; Fiscal Year: 2013
    ..histologically, and early stage melanoma is defined as radial growth phase (RGP), which is confined to the epidermis. In contrast, later stage vertical growth phase (VGP) is characterized by invasion of melanoma cells into the ..
  34. Mechanisms of Adult Taste Bud Regeneration
    Linda A Barlow; Fiscal Year: 2013
    ..The current model of taste bud regeneration has been adopted primarily from studies of epidermis: presumed taste bud stem cells self-renew and generate transit amplifying cells, which together comprise the ..
  35. Cell Adhesion and Cytoskeletal Dynamics in Skin
    Elaine Fuchs; Fiscal Year: 2013
    ..Elucidating how microtubules (MTs) link to AJs is important for understanding how the stratified epidermis maintains a single layer of dividing cells, and how spindle polarity changes when stem cells are activated to ..
  36. microRNA Control of Wound Healing
    Chandan K Sen; Fiscal Year: 2013
    ..conditional ablation of Dicer in mice (K14CreERtam Dicer-/-) results in depletion of mature miRs in the skin epidermis and compromises re-epithelialization of excisional wounds. Hypothesis 1...
  37. Skin Stem Cells: Purification and Characterization
    Elaine Fuchs; Fiscal Year: 2013
    ..Adult epithelial SCs of the skin reside within the innermost (basal) layer of the interfollicular epidermis (IFE) as well as the bulge, a niche located within the hair follicle (HF)...
  38. Fanconi Anemia and HPV Transformation
    Susanne I Wells; Fiscal Year: 2013
    ..Of the solid tumors, most are squamous cell carcinomas, which arise from the main cell type within the epidermis, the human keratinocyte...
  39. Therapeutic Intervention of Lewisite-Mediated Cutaneous Blistering-Inflammation
    Mohammad Athar; Fiscal Year: 2013
    ..in epidermis. In addition, proteins involved in the regulation of water/glycerin transport, the aquaporins, will be studied...
  40. Mechanisms of Genetic Reversion in Ichthyosis With Confetti
    Keith A Choate; Fiscal Year: 2013
    ..varying proportions of revertant or normal keratinocytes are mixed with mutant IWC or EI cells and resulting epidermis is assayed for composition by each cell type over time. 3...
  41. Role of vitamin D and calcium signaling in wound healing
    DANIEL DAVID BIKLE; Fiscal Year: 2013
    ..follicle provide keratinocytes not only for hair follicle formation but also for the reepithelialization of the epidermis following wounding. The function of these bulge stem cells is regulated by VDR...
  42. Characterization of persistent hyperemic foci and their role in photocarcinogenes
    Raymond L Konger; Fiscal Year: 2013
    ..In the second aim, we will examine whether the epidermis overlying early hyperemic areas exhibit increased mutations of the key DNA damage regulator, p53, which is known ..
  43. Aging Stratum Corneum pH and Barrier Function
    Theodora M Mauro; Fiscal Year: 2012
    ..previously found that NHE1 protein levels and full- thickness SC acidity are decreased in moderately aged mouse epidermis. We now propose to define the relative roles of NHE1 and sPLA2 in acidifying specific regions of the SC in young ..
  44. GR Impairment in Carconogenesis: Tumor Suppressor Role
    THOMAS SLAGA; Fiscal Year: 2009
    ..The constitutively nuclear overexpression and activation of GR in the epidermis dramatically inhibited skin tumor development in K5...
  45. Role of Mitochondria in Estrogen Action and Cellular Senescence
    Michael C Velarde; Fiscal Year: 2013
    ..e. cellular senescence) in mammalian skin epidermis by using a transgenic mouse model deficient in superoxide dismutase 2 (sod2);(b) address the molecular mechanism ..
  46. Protein Kinase D and Epidermal Structure and Function
    Wendy B Bollag; Fiscal Year: 2010
    ..BCCs, which make up approximately 80% of the NMSCs, arise from basal keratinocytes in the epidermis of the skin...
  47. Development of the renin-expressing cell
    Maria L Sequeira Lopez; Fiscal Year: 2010
    ..mice expressing ere recombinase in precursors of neuroectoderm (nestin-cre), neural crest cells (wnt1-cre) and epidermis (K14-cre)...
  48. Tissue Oxygenation and Wound Healing
    Chandan K Sen; Fiscal Year: 2013
    ..2 Lactate drives miR-210 expression. Aim 3: Elevated miR-210 in the epidermis of ischemic human wound edge tissue is associated with poor healing outcome in a setting of standard clinical ..
  49. Role of Beta 1 containing Integrins in Cutaneous Basement Membrane Organization
    Srikala Raghavan; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): In skin epidermis, cell migration and basement membrane assembly and organization are central to many biological and pathological processes, including hair follicle morphogenesis, wound healing and ..
  50. Trim32 Regulation of Piasy in Skin Homeostasis
    Molly F Kulesz-Martin; Fiscal Year: 2013
    ..Trim32 is elevated in psoriasis tissue samples compared to non-lesional control epidermis. Trim32 negatively regulates the pro-apoptotic Piasy protein, a repressor of NF-kB, STAT, and SMAD transcription ..
  51. Regulation of human skin homeostasis by histone arginine methylation regulators
    Xiaomin Bao; Fiscal Year: 2013
    ..We will therefore define the role of PRMT1 in epidermis undergoing neoplastic transformation...
  52. Role of DeltaNp63alpha in Vitamin D Mediated Cell Survival in Skin Cancer
    Madhavi P Kadakia; Fiscal Year: 2013
    ..Both [unreadable]Np63a and VDR are present in the basal layer of the epidermis and are critical to the maintenance of a stratified epidermis...
  53. Integration of Immunochemical, Genomic & Transcriptomic Analyses of Human Skin
    FRANK LAMBERT contact RICE; Fiscal Year: 2010
    ..5 to assess the density of sensory endings strictly in the epidermis. This phase I STTR submission proposes to vastly expand the utility of skin biopsies by integrating multi-..
  54. Role of FATP4 in Skin Development
    Jeffrey H Miner; Fiscal Year: 2012
    ..wrfr mice are born with taut, shiny skin, a thickened epidermis, and a defective skin barrier...
  55. Matrix Metalloproteinase Regulation of Leukocyte Infiltration during Wound Repair
    LISA JOY MCCAWLEY; Fiscal Year: 2013
    ..MMP3 is upregulated in the epidermis during processes undergoing tissue remodeling...
  56. Epha/Ephrin - A Signaling in Epidermal Differentiation and Disease
    Spiro Getsios; Fiscal Year: 2013
    ..Species differences in the character and molecular composition of EphA receptors in the epidermis necessitate the application of experimental approaches that take advantage of human cells...
  57. Functional Analysis of the Hairless Protein
    Angela M Christiano; Fiscal Year: 2012
    ..into a transcriptional regulatory pathway, we performed a microarray analysis of rh/rh versus wild-type mouse epidermis to identify genes that are regulated by hairless...
  58. Function and Targeting of CCR6/CCL20 in Autoimmune Psoriasiform Skin Disease
    Sam T Hwang; Fiscal Year: 2013
    ..GDL) T cells, distinct from resident dendritic epidermal ?[unreadable] T cells (DETC), accumulates in murine epidermis following exposure to IL23 and is a major producer of IL22 and IL17 in murine epidermis...
  59. Mechanism of Cancer Prevention by Weight Control
    WEIQUN GEORGE WANG; Fiscal Year: 2011
    ..and PI3K-Akt-antiapoptosis, suggesting a crosstalk mechanism in diminishing TPA-promoted signaling in the epidermis of mice...
  60. Extrinsic Regulation of Epidermal Homeostatasis
    Valerie Horsley; Fiscal Year: 2013
    ..Epithelial stem cells are responsible for the maintenance and regeneration of self-renewing tissues such as epidermis, hair follicle, and mammary gland...
  61. TRP Ca2+ Channels in the Skin
    Haoxing Xu; Fiscal Year: 2013
    ..Overall, the close interaction of TRPV3 and EGFR signaling on normal epidermis and the well-demonstrated role of EGFR signaling in cancer suggest a role of TRPV3 in epithelial tumorigenesis...
  62. Topical DNA immunization by modifying the hair follicle cycle
    Zhengrong Cui; Fiscal Year: 2011
    ..encoded by the plasmid was largely confined in the hair follicles and only occasionally in other cells in the epidermis. Moreover, the finding that the presence of normal hair follicles in the skin was required for a topical DNA ..
  63. SEGMENTATION AND PATTERNING IN DROSOPHILA
    Eric Wieschaus; Fiscal Year: 2000
    ..pattern of segmentation genes at the blastoderm stage and the final pattern of cell differentiation in the epidermis. Much of the work will concentrate on armadillo, the Drosophila homolog of vertebrate beta- catenin, which ..
  64. Stratum Corneum Acidification in the Neonate
    Kenneth Feingold; Fiscal Year: 2007
    ..homeostasis and SC integrity and cohesion, and that a neutral SC pH increases the levels of cytokines in the epidermis. An acidic surface pH is achieved in the absence of exogenous mechanisms, such as surface lipids, eccrine gland ..
  65. PHOSPHOLIPASE C/PROTEIN KINASE C IN PSORIATIC EPIDERMIS
    Gary Fisher; Fiscal Year: 1993
    ..of this proposal are to understand the mechanisms that regulate growth and differentiation of adult human epidermis and thereby provide a rationale for the design of effective therapeutic modalities...
  66. PROGRAM PROJECT GRANT
    Jouni Uitto; Fiscal Year: 2006
    ..molecular basis of various forms of epidermolysis bullosa (EB) and other selected genodermatoses affecting the epidermis. The proposed studies are designed to test the hypothesis that genetic lesions in structural genes expressed in ..
  67. Desmoglein 2 Function in Cell Adhesion and Pemphigus
    MY MAHONEY; Fiscal Year: 2003
    ..Desmogleins (Dsg) are the transmembrane adhesive components of the desmosomes. In the epidermis, Dsg2 and Dsg3 are expressed in the proliferative basal keratinocytes while Dsgl is expressed in the upper ..
  68. REGULATION OF EPIDERMAL DIFFERENTATION BY PTHRP
    John Foley; Fiscal Year: 2004
    DESCRIPTION: (Adapted from the applicant's abstract) - The skin is made up of two distinct layers: the epidermis and dermis...
  69. Communication Between Laminin 5 and Gap Junctions
    William Carter; Fiscal Year: 2005
    Epithelial cells assemble adhesive and communicating cell junctions at substrate and cell-cell contacts. In epidermis, cell junctions integrate individual keratinocytes into tissue with barrier and communicating functions...
  70. ADHESION MOLECULES AND EPIDERMAL STRATIFICATION
    ROBERT LAVKER; Fiscal Year: 2001
    The protective function of the epidermis depends critically on the normal differentiation of the keratinocytes. Cell to cell adhesion is essential in this process...
  71. PATHOGENESIS OF MYCOSIS FUNGOIDES
    Irwin Braverman; Fiscal Year: 1992
    ..accepted that the epidermal microenvironment in CTCL is responsible for the accumulation of T cells within the epidermis. However EM and light microscopic studies from this laboratory have shown that a subset of epidermal dendritic ..
  72. SECRETORY IMMUNE SYSTEM AND THE SKIN
    J Huff; Fiscal Year: 1990
    ..in IgA dermatoses and in the immunopathology of the skin, we have developed a hypothesis that the normal human epidermis may interact with IgA by a receptor mediated mechanism...
  73. DERMATOREMEDIATION OF IRON OVERLOAD
    Leonard M Milstone; Fiscal Year: 2010
    ..Normally, 20% of absorbed iron is eliminated through epidermal desquamation. The central hypothesis is that if epidermis can be made to act as a sink for internal iron, then epidermal shedding might reduce the body burden of iron...