rod outer segments

Summary

Summary: The portion of the retinal rod cell between the inner segment and the pigment epithelium layer of the retina.

Top Publications

  1. pmc Rhodopsin signaling and organization in heterozygote rhodopsin knockout mice
    Yan Liang
    Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:48189-96. 2004
  2. ncbi Reduced G protein-coupled signaling efficiency in retinal rod outer segments in response to n-3 fatty acid deficiency
    Shui Lin Niu
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852, USA
    J Biol Chem 279:31098-104. 2004
  3. pmc Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography
    Stephan Nickell
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 177:917-25. 2007
  4. pmc G protein-coupled receptor rhodopsin
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Biochem 75:743-67. 2006
  5. ncbi Phospholipase D from photoreceptor rod outer segments is a downstream effector of RhoA: evidence of a light-dependent mechanism
    Gabriela A Salvador
    Instituto de Investigaciones Bioquimicas de Bahia Blanca, Universidad Nacional del Sur and Consejo Nacional de Investigaciones Cientificas y Tecnicas, C C 857, B8000FWB Bahia Blanca, Argentina
    Exp Eye Res 83:202-11. 2006
  6. ncbi Dark adaptation and the retinoid cycle of vision
    T D Lamb
    Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra ACT 2601, Australia
    Prog Retin Eye Res 23:307-80. 2004
  7. ncbi Low docosahexaenoic acid levels in rod outer segments of rats with P23H and S334ter rhodopsin mutations
    Robert E Anderson
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Mol Vis 8:351-8. 2002
  8. ncbi Differential modification of phosducin protein in degenerating rd1 retina is associated with constitutively active Ca2+/calmodulin kinase II in rod outer segments
    Stefanie M Hauck
    GSF National Research Centre for Environment and Health, Institute of Human Genetics, Neuherberg 85764, Germany
    Mol Cell Proteomics 5:324-36. 2006
  9. pmc Mathematical model of the spatio-temporal dynamics of second messengers in visual transduction
    D Andreucci
    Dipartimento di Metodi e Modelli Matematici, Universita di Roma La Sapienza, 00161 Rome, Italy
    Biophys J 85:1358-76. 2003
  10. ncbi Light-induced changes in protein nitration in photoreceptor rod outer segments
    Vikram Palamalai
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA
    Mol Vis 12:1543-51. 2006

Detail Information

Publications214 found, 100 shown here

  1. pmc Rhodopsin signaling and organization in heterozygote rhodopsin knockout mice
    Yan Liang
    Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:48189-96. 2004
    Rhodopsin (Rho) resides within internal membrane structures called disc membranes that are found in the rod outer segments (ROS) of photoreceptors in the retina...
  2. ncbi Reduced G protein-coupled signaling efficiency in retinal rod outer segments in response to n-3 fatty acid deficiency
    Shui Lin Niu
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Rockville, Maryland 20852, USA
    J Biol Chem 279:31098-104. 2004
    ....
  3. pmc Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography
    Stephan Nickell
    Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, D 82152 Martinsried, Germany
    J Cell Biol 177:917-25. 2007
    ..Spacers were found distributed throughout the discs, including regions that are distant from the rim region of discs...
  4. pmc G protein-coupled receptor rhodopsin
    Krzysztof Palczewski
    Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4965, USA
    Annu Rev Biochem 75:743-67. 2006
    ..in the retina, its specific localization in the internal disks of the photoreceptor structures [termed rod outer segments (ROS)], and the lack of other highly abundant membrane proteins allow rhodopsin to be examined in the native ..
  5. ncbi Phospholipase D from photoreceptor rod outer segments is a downstream effector of RhoA: evidence of a light-dependent mechanism
    Gabriela A Salvador
    Instituto de Investigaciones Bioquimicas de Bahia Blanca, Universidad Nacional del Sur and Consejo Nacional de Investigaciones Cientificas y Tecnicas, C C 857, B8000FWB Bahia Blanca, Argentina
    Exp Eye Res 83:202-11. 2006
    Photoreceptor cells contain rod outer segments (ROS) which are specialized light-sensitive organelles. The biological function of ROS is to generate a photoresponse, which occurs via the classic transducin-mediated pathway...
  6. ncbi Dark adaptation and the retinoid cycle of vision
    T D Lamb
    Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra ACT 2601, Australia
    Prog Retin Eye Res 23:307-80. 2004
    ..bleach the recovery is limited by the rate at which 11-cis retinal is delivered to opsin in the bleached rod outer segments. We present a mathematical model that successfully describes a wide range of results in human and other ..
  7. ncbi Low docosahexaenoic acid levels in rod outer segments of rats with P23H and S334ter rhodopsin mutations
    Robert E Anderson
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Mol Vis 8:351-8. 2002
    ..In the present study, we analyzed the ROS fatty composition of several lines of transgenic rats with P23H and S334ter rhodopsin mutations. Lines were chosen that have different rates of retinal degeneration...
  8. ncbi Differential modification of phosducin protein in degenerating rd1 retina is associated with constitutively active Ca2+/calmodulin kinase II in rod outer segments
    Stefanie M Hauck
    GSF National Research Centre for Environment and Health, Institute of Human Genetics, Neuherberg 85764, Germany
    Mol Cell Proteomics 5:324-36. 2006
    ..Given the increased rod calcium levels present in the rd1 mutation, calcium-evoked overactivation of this kinase may be an early and long sought for step in events leading to photoreceptor degeneration in the rd1 mouse...
  9. pmc Mathematical model of the spatio-temporal dynamics of second messengers in visual transduction
    D Andreucci
    Dipartimento di Metodi e Modelli Matematici, Universita di Roma La Sapienza, 00161 Rome, Italy
    Biophys J 85:1358-76. 2003
    ..This approach represents a tool to analyze point-wise signaling dynamics without requiring averaging over the entire cell by global Michaelis-Menten kinetics...
  10. ncbi Light-induced changes in protein nitration in photoreceptor rod outer segments
    Vikram Palamalai
    Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND, USA
    Mol Vis 12:1543-51. 2006
    ..To better understand the role of protein nitration in photoreceptor cell death induced by intense light, we examined retinal protein nitration and identified target proteins in rod outer segments (ROS).
  11. ncbi Detecting molecular interactions that stabilize native bovine rhodopsin
    K Tanuj Sapra
    Center for Biotechnology, University of Technology, 01307 Dresden, Germany
    J Mol Biol 358:255-69. 2006
    ..These changes highlight the structural importance of this disulfide bond and may form the basis of dysfunctions associated with its absence...
  12. ncbi Transducin subunit stoichiometry and cellular distribution in rod outer segments
    James W Clack
    Department of Biology, Indiana University, Purdue University at Indianapolis, 4601 Central Ave, Columbus, IN 47203, USA
    Cell Biol Int 30:829-35. 2006
    ..elute G(alphat) and that there is an overall three to fourfold molar excess of G(betagamma) to G(alphat) in rod outer segments. These results suggest that the production and/or turnover of G(alphat), G(beta), and G(gamma) in the rod ..
  13. ncbi Cholesterol-dependent association of caveolin-1 with the transducin alpha subunit in bovine photoreceptor rod outer segments: disruption by cyclodextrin and guanosine 5'-O-(3-thiotriphosphate)
    Michael H Elliott
    Dean A McGee Eye Institute, Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Biochemistry 42:7892-903. 2003
    ..Detergent-resistant membranes isolated from rod outer segments (ROS) have been previously shown to contain the photoreceptor G-protein, transducin...
  14. ncbi Alterations in retinal rod outer segment fatty acids and light-damage susceptibility in P23H rats
    Ina Rea Bicknell
    Petticrew Research Laboratory, Department of Biochemistry and Molecular Biology, School of Medicine, Wright State University, Cox Institute, Dayton, OH 45429, USA
    Mol Vis 8:333-40. 2002
    To determine whether dietary-induced alterations in the long-chain polyunsaturated fatty acid content of retinal rod outer segments (ROS) of P23H rats, a transgenic model of retinitis pigmentosa (RP), prolongs photoreceptor cell life.
  15. ncbi In vivo regulation of phosphoinositide 3-kinase in retina through light-induced tyrosine phosphorylation of the insulin receptor beta-subunit
    Raju V S Rajala
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:43319-26. 2002
    ..We suggest that the physiological role of this process may be to provide neuroprotection of the retina against light damage by activating proteins that protect against stress-induced apoptosis...
  16. ncbi Lumi I --> Lumi II: the last detergent independent process in rhodopsin photoexcitationt
    Jacqueline Epps
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, Santa Cruz, California 95064, USA
    Photochem Photobiol 82:1436-41. 2006
    ..The blue shift is similar to one induced by chloride ion in the E181Q rhodopsin mutant and may indicate that the ionization state of Glu181 in rhodopsin is affected by detergent...
  17. ncbi Mutation R238E in transducin-alpha yields a GTPase and effector-deficient, but not dominant-negative, G-protein alpha-subunit
    Brandy Barren
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    Mol Vis 12:492-8. 2006
    ..We thought to further investigate a novel mutant of the transducin-alpha subunit, R238E, that was recently reported to be a dominant-negative inhibitor of the rhodopsin/transducin/PDE visual system...
  18. ncbi Structure of rhodopsin in monolayers at the air-water interface: a PM-IRRAS and X-ray reflectivity study
    Hugo Lavoie
    Departement de Chimie Biologie, Universite du Quebec a Trois Rivieres, Quebec, Canada
    Biochemistry 41:13424-34. 2002
    ..In addition, the PM-IRRAS spectra of rod outer segment disk membranes in monolayers suggest that rhodopsin also retained its secondary structure in these films...
  19. ncbi Expression of metallothionein isoforms in human chorioretinal complex
    David J Tate
    Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, Louisiana 70112, USA
    Curr Eye Res 24:12-25. 2002
    ..To determine the relative expression of metallothionein isoforms and their differential induction by oxidative stress in cultured RPE cells and to localize the isoforms in the human chorioretinal complex...
  20. ncbi Species-specific subcellular localization of RPGR and RPGRIP isoforms: implications for the phenotypic variability of congenital retinopathies among species
    Timur A Mavlyutov
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Hum Mol Genet 11:1899-907. 2002
    ....
  21. pmc Suramin affects coupling of rhodopsin to transducin
    Nicole Lehmann
    Institut für Molekulare Medizin und Zellforschung, Albert Ludwigs Universitat, D 79104 Freiburg, Germany
    Biophys J 82:793-802. 2002
    ..Disruption of membrane anchoring may contribute to some of the effects of suramin exerted on other G-proteins...
  22. ncbi Signal-dependent translocation of transducin, RGS9-1-Gbeta5L complex, and arrestin to detergent-resistant membrane rafts in photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    Curr Biol 12:421-5. 2002
    ..These findings could reveal a novel mechanism utilized by the cells for regulation of G protein-mediated signal transduction...
  23. pmc Activation of rhodopsin kinase
    Nina E M McCarthy
    Department of Biochemistry, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, Hants, U K
    Biochem J 363:359-64. 2002
    ..These results led to the conclusion that all the derivatives of opsin, which contain the Schiff base linkage with the all-trans-retinylidene moiety, retain the ability to activate RK...
  24. ncbi Reversal of functional loss in the P23H-3 rat retina by management of ambient light
    Camilla Jozwick
    CNS Stability and Degeneration Group and ARC Centre of Excellence in Vision Science, Research School of Biological Sciences, Australian National University, PO Box 475, Canberra, ACT 2601, Australia
    Exp Eye Res 83:1074-80. 2006
    ..Restriction of ambient light may benefit comparable human forms of retinal degeneration in two ways, by reducing the rate of photoreceptor death and by inducing functional recovery in surviving photoreceptors...
  25. ncbi Electrostatic properties of membrane lipids coupled to metarhodopsin II formation in visual transduction
    Yin Wang
    Department of Physics, University of Arizona, Tucson, AZ 85721, USA
    J Am Chem Soc 124:7690-701. 2002
    ..Moreover, they yield knowledge of how membrane lipid-protein interactions involving acidic phospholipids such as PS and neutral polyunsaturated DHA chains are implicated in key biological functions such as vision...
  26. ncbi Relative orientation between the beta-ionone ring and the polyene chain for the chromophore of rhodopsin in native membranes
    Paul J R Spooner
    The Biomembrane Structure Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Biochemistry 41:7549-55. 2002
    ..The more precise distance measurement made here between C8 and C18 (2.95 A) predicts that the chain is twisted out-of-plane with respect to the ring by a modest amount (C5-C6-C7-C8 torsion angle = -28 +/- 7 degrees )...
  27. ncbi Effect of light and protein phosphorylation on photoreceptor rod outer segment acyltransferase activity
    P I Castagnet
    Institute de Investigaciones Bioquímicas, Universidad Nacional del Sur y Consejo Nacional de Investigaciones Científicas y Técnicas, B8000FWB Bahia Blanca, Argentina
    Arch Biochem Biophys 403:83-91. 2002
    b>Rod outer segments (ROS) exhibit high acyltransferase (AT) activity, the preferred substrate of which being lysophosphatidylcholine...
  28. ncbi Light-induced changes in the structure and accessibility of the cytoplasmic loops of rhodopsin in the activated MII state
    Thorsten Mielke
    Biophysics Group, Department of Physics, Freie Universitat Berlin, Arnimallee 14, D 14195 Berlin, Germany
    Biochemistry 41:7875-84. 2002
    ....
  29. ncbi Distribution and translocation of photoreceptor Gbetagamma-phosducin system in medaka retina
    Shintaro Yamamoto
    Department of Earth and Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
    Photochem Photobiol 83:35-9. 2007
    ..In rod outer segments (ROS), PD-R reactivity was stronger in light-adapted retina than in dark-adapted retina...
  30. pmc Functional and structural characterization of rhodopsin oligomers
    Beata Jastrzebska
    Department of Pharmacology, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 281:11917-22. 2006
    ..Our studies provide direct comparisons between signaling properties of Meta II in different quaternary complexes...
  31. ncbi Agonists and partial agonists of rhodopsin: retinal polyene methylation affects receptor activation
    Reiner Vogel
    Biophysics Group, Institut für Molekulare Medizin und Zellforschung, Albert Ludwigs Universitat Freiburg, Hermann Herder Strasse 9, D 79104 Freiburg, Germany
    Biochemistry 45:1640-52. 2006
    ..The 9-methyl group appears therefore essential for the correct positioning of retinal to link protonation of the cytoplasmic motif with protonation of Glu 113 during receptor activation...
  32. pmc The supramolecular structure of the GPCR rhodopsin in solution and native disc membranes
    Kitaru Suda
    ME Muller Institute for Microscopy, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Membr Biol 21:435-46. 2004
    Rhodopsin, the prototypical G-protein-coupled receptor, which is densely packed in the disc membranes of rod outer segments, was proposed to function as a monomer...
  33. ncbi From candelas to photoisomerizations in the mouse eye by rhodopsin bleaching in situ and the light-rearing dependence of the major components of the mouse ERG
    Arkady L Lyubarsky
    Department of Ophthalmology, University of Pennsylvania, F M Kirby Center for Molecular Ophthalmology, Stellar Chance Building, Room 309B, 422 Curie Boulevard, Philadelphia, PA 19104 6069, USA
    Vision Res 44:3235-51. 2004
    ..The intensity that produces a half-saturating rod b-wave response is 0.3-0.6 photoisomerizations rod(-1), and the amplification constant of the rod a-wave is 5-6 s(-2) photoisomerization(-1), with little dependence on the strain...
  34. pmc Evaluation of the 17-kDa prenyl-binding protein as a regulatory protein for phototransduction in retinal photoreceptors
    Angela W Norton
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824 2617, USA
    J Biol Chem 280:1248-56. 2005
    ....
  35. pmc Constraints on the conformation of the cytoplasmic face of dark-adapted and light-excited rhodopsin inferred from antirhodopsin antibody imprints
    Brian W Bailey
    Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717 3520, USA
    Protein Sci 12:2453-75. 2003
    ....
  36. ncbi Chemical modification of transducin with dansyl chloride hinders its binding to light-activated rhodopsin
    Ana Kosoy
    Departamento de Quimica, Universidad Simon Bolivar, Caracas 1081 A, Venezuela
    J Biochem Mol Biol 37:260-7. 2004
    Transducin (T), the heterotrimeric guanine nucleotide binding protein in rod outer segments, serves as an intermediary between the receptor protein, rhodopsin, and the effector protein, cGMP phosphodiesterase...
  37. ncbi Probing rhodopsin-transducin interactions by surface modification and mass spectrometry
    Xin Wang
    Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 43:11153-62. 2004
    ..In addition, we show evidence of interaction between the end of the C-terminal tail of rhodopsin and G(t)alpha in the unactivated state of rhodopsin...
  38. ncbi Analysis of the molecular interaction of the farnesyl moiety of transducin through the use of a photoreactive farnesyl analogue
    Ken ichi Hagiwara
    Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0032, Japan
    Biochemistry 43:300-9. 2004
    ..These results illustrate that, rather than being a static membrane anchor, the farnesyl moiety plays an active role in the dynamics of protein-protein and protein-membrane interactions at defined steps in the signal transduction process...
  39. ncbi Changes in retinal pigment epithelial gene expression induced by rod outer segment uptake
    Itay Chowers
    Guerrieri Center for Genetic Engineering and Molecular Ophthalmology, Wilmer Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Invest Ophthalmol Vis Sci 45:2098-106. 2004
    ..The goal of this study was to find additional genes involved in ROS uptake and degradation by identifying ROS-induced gene expression changes...
  40. ncbi Direct binding of visual arrestin to microtubules determines the differential subcellular localization of its splice variants in rod photoreceptors
    K Saidas Nair
    Department of Molecular and Cellular Pharmacology and Neuroscience Program, University of Miami, Miami, Florida 33136, USA
    J Biol Chem 279:41240-8. 2004
    ....
  41. ncbi Immobilization of native membrane-bound rhodopsin on biosensor surfaces
    Jasmina Minic
    INRA, Neurobiologie de l Olfaction et de la Prise Alimentaire, Equipe Récepteurs et Communication Chimique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Biochim Biophys Acta 1724:324-32. 2005
    ..Therefore, such receptor immobilization method is attractive for a range of applications, especially in the field of GPCR biosensors...
  42. ncbi Enhancement of G protein-coupled signaling by DHA phospholipids
    Drake C Mitchell
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892 8115, USA
    Lipids 38:437-43. 2003
    ..Our results indicate that efficient and rapid propagation of G protein-coupled signaling is optimized by DHA phospholipid acyl chains...
  43. ncbi Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities
    Thierry J Horner
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, Carolina 27599 7090, USA
    J Biol Chem 280:28241-50. 2005
    ..b>Rod outer segments isolated from bovine retina phosphorylated the FLAG-tagged GRKs in the presence of dibutyryl-cAMP, ..
  44. ncbi Retarded outer segment development in TrkB knockout mouse retina organ culture
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 9:18-23. 2003
    ..To determine the effects of trkB deficiency in the mouse retina on photoreceptor development and retinal organization, in the absence of confounding systemic effects...
  45. ncbi A morphometric study of light-induced damage in transgenic rat models of retinitis pigmentosa
    Dana K Vaughan
    University of Wisconsin Oshkosh, Department of Biology, Oshkosh, Wisconsin 54901, USA
    Invest Ophthalmol Vis Sci 44:848-55. 2003
    ..To determine relative susceptibility to, and regional variation of, light-induced retinal damage in two rhodopsin-mutant rat models of retinitis pigmentosa, using slow- and fast-degenerating lines...
  46. ncbi Arrestin translocation is induced at a critical threshold of visual signaling and is superstoichiometric to bleached rhodopsin
    Katherine J Strissel
    Department of Ophthalmology, Harvard Medical School, The Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    J Neurosci 26:1146-53. 2006
    ..Second, the amount of arrestin entering rod outer segments under these conditions is superstoichiometric to the amount of photoactivated rhodopsin, exceeding it by at ..
  47. ncbi Retinal dysfunction in patients with chronic Chagas' disease is associated to anti-Trypanosoma cruzi antibodies that cross-react with rhodopsin
    Silvia C Matsumoto
    Department of Neurology, Hospital Dr Teodoro Alvarez, Buenos Aires, Argentina
    FASEB J 20:550-2. 2006
    ....
  48. pmc The glutamic acid-rich protein-2 (GARP2) is a high affinity rod photoreceptor phosphodiesterase (PDE6)-binding protein that modulates its catalytic properties
    Dana C Pentia
    Department of Biochemistry and Molecular Biology, University of New Hampshire, 46 College Road, Durham, NH 03824 2617, USA
    J Biol Chem 281:5500-5. 2006
    ....
  49. ncbi Acceleration of key reactions as a strategy to elucidate the rate-limiting chemistry underlying phototransduction inactivation
    Matthew J Kennedy
    Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA
    Invest Ophthalmol Vis Sci 44:1016-22. 2003
    ..A reconstituted system was used to establish a strategy to determine the rate-limiting chemistry responsible for recovery of the dim-flash response in rod photoreceptors...
  50. ncbi Retinal regional differences in photoreceptor cell death and regeneration in light-lesioned albino zebrafish
    Thomas S Vihtelic
    Center for Zebrafish Research, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Exp Eye Res 82:558-75. 2006
    ..This suggests that the levels of photoreceptor damage may directly influence cell responses in the underlying retinal layers...
  51. ncbi [Peculiarities of rhodopsin photoconversion at the early stages of photolysis]
    T B Fel'dman
    Institute of Biochemical Physics, Russian Acad Sci, 119991, Moscow, 4 Kosygin St, Russia
    Ross Fiziol Zh Im I M Sechenova 89:113-22. 2003
    ..The data suggest that appearance of more than one intermediate at each stage of the rhodopsin photolysis reflects existence of multiple conformation states of the rhodopsin molecule in the course of its photoconversion...
  52. ncbi Light-dependent translocation of arrestin in the absence of rhodopsin phosphorylation and transducin signaling
    Ana Mendez
    Zilkha Neurogenetic Institute, The Mary D Allen Laboratory for Vision Research, Beckman Macular Research Center, Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089 2821, USA
    J Neurosci 23:3124-9. 2003
    ..region of dark-adapted rod photoreceptors, whereas light induces redistribution of arrestin to the rod outer segments. The mechanism by which arrestin redistributes in response to light is not known, but it is thought to be ..
  53. pmc Effect of packing density on rhodopsin stability and function in polyunsaturated membranes
    Shui Lin Niu
    Section of Fluorescence Studies, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biophys J 89:1833-40. 2005
    ..These observations imply that densely packed rhodopsin may impede the diffusion and conformational changes of rhodopsin, which could reduce the speed of visual transduction...
  54. ncbi G-protein alpha and beta-gamma subunits interact with conformationally distinct signaling states of rhodopsin
    Maureen A Downs
    Department of Ophthalmology, Saint Louis University School of Medicine, St Louis, MO 63104, USA
    Vision Res 46:4442-8. 2006
    ..Analysis of the rates of Meta II decay confirms that the conformational states of Meta II when bound to Gtalpha and Gtbetagamma represent distinct signaling states of rhodopsin...
  55. ncbi Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in bovine rod outer segments
    Isabella Panfoli
    Department of Biology, University of Genoa, Genova, Italy
    Invest Ophthalmol Vis Sci 48:978-84. 2007
    ..In this study, the presence and functional role of the adenosine diphosphoribosyl (ADPR)-cyclase-cyclic ADP-ribose (cADPR) system in bovine retinal rod outer segments (ROS) was investigated.
  56. ncbi A methyl group at C7 of 11-cis-retinal allows chromophore formation but affects rhodopsin activation
    Laia Bosch
    Departament d Enginyeria Quimica, Universitat Politecnica de Catalunya, 08222 Terrassa, Catalonia, Spain
    Vision Res 46:4472-81. 2006
    ..Molecular dynamics simulations suggest coupling of the C7 methyl group and the beta-ionone ring with Met207 in transmembrane helix 5 in agreement with recent experimental results...
  57. ncbi Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination
    Hongman Song
    Department of Ophthalmology, West Virginia University School of Medicine and West Virginia University Eye Institute, Morgantown, West Virginia 26506, USA
    J Biol Chem 282:23613-21. 2007
    ..These results support the role of phosducin in regulating signaling in the rod outer segment compartment and suggest distinct functions for phosphorylation sites 54 and 71...
  58. ncbi Tyrosinase biosynthesis and trafficking in adult human retinal pigment epithelial cells
    Sylvie Julien
    Section for Experimental Vitreoretinal Surgery, University Eye Hospital Tuebingen, Tuebingen, Germany
    Graefes Arch Clin Exp Ophthalmol 245:1495-505. 2007
    ..In this study, we investigated if phagocytosis of rod outer segments (ROS) can increase tyrosinase expression in vitro.
  59. pmc Functional characterization of rhodopsin monomers and dimers in detergents
    Beata Jastrzebska
    Department of Ophthalmology, University of Washington, Seattle, Washington 98195, USA
    J Biol Chem 279:54663-75. 2004
    ..High expression levels of Rho in the disc membranes of rod outer segments and the propensity of Rho to form higher oligomeric structures are evident from atomic force microscopy, ..
  60. ncbi Mesoscopic Monte Carlo simulations of stochastic encounters between photoactivated rhodopsin and transducin in disc membranes
    Daniele Dell'Orco
    Department of Chemistry, University of Modena and Reggio Emilia and Dulbecco Telethon Institute, via Campi 183 41100 Modena, Italy
    J Phys Chem B 112:4419-26. 2008
    The issue of how the molecular organization of rod outer segments (ROS) discs affects the initial timing of the photoresponse in vertebrates has been recently raised by novel structural findings that raise doubts about the classical ..
  61. ncbi Defective cone photoreceptor cytoskeleton, alignment, feedback, and energetics can lead to energy depletion in macular degeneration
    M S Marion S Eckmiller
    C and O Vogt Institute for Brain Research, University Clinic, Heinrich Heine University of Düsseldorf Medical School, Postfach 101007, Dusseldorf 40001, Germany
    Prog Retin Eye Res 23:495-522. 2004
    ..cytoskeletal systems at the connecting cilium of rods and cones and at the multiple incisures of rod outer segments (OSs)...
  62. ncbi Anatomy and histology of the brain and sense organs of the Antarctic eel cod Muraenolepis microps (Gadiformes; Muraenolepididae)
    J T Eastman
    Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701 2979, USA
    J Morphol 250:34-50. 2001
    ..Muraenolepis has a pure rod retina like many deep-sea species but lacks the superimposed layers of rod outer segments. The histology of the nonvisual sense organs, especially the olfactory and external taste systems, are well-..
  63. ncbi Immunocytochemical localization of the NMDA-R2A receptor subunit in the cat retina
    D J Goebel
    Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, 540 E Canfield, Detroit, MI 48201, USA
    Brain Res 808:141-54. 1998
    ..the R2B subunits were found to be present in the outer segments of cone photoreceptors and to the tips of rod outer segments. Although the function of these receptor subunits in rod and cone photoreceptors remains to be determined, ..
  64. ncbi Retinal vascular changes induced by the oxidative stress of alpha-tocopherol deficiency contrasted with diabetic microangiopathy
    W G Robison
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892 2735, USA
    J Ocul Pharmacol Ther 16:109-20. 2000
    ..At 6 and 8 months, the deficient rats exhibited extensive shortening and disarray of rod outer segments (ROS), marked loss of photoreceptor cells, and pronounced increases in the numbers of granules with ..
  65. ncbi Visual optics in toads (Bufo americanus)
    U Mathis
    Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853
    J Comp Physiol A 163:201-13. 1988
    ..increased light sensitivity could partly be explained with an increase in aperture and an increase in red rod outer segments. To fully explain the higher light sensitivity in adult toads, changes in neuronal parameters had to be ..
  66. ncbi Rod photoreceptor neurite sprouting in retinitis pigmentosa
    Z Y Li
    Department of Ophthalmology, University of Washington, Seattle 98195, USA
    J Neurosci 15:5429-38. 1995
    ..Rhodopsin immunolabeling in the normal retinas was restricted to the rod outer segments. In the RP retinas, rhodopsin was present in shortened rod outer segments and in the surface membranes of ..
  67. ncbi Activation of arrestin: requirement of phosphorylation as the negative charge on residues in synthetic peptides from the carboxyl-terminal region of rhodopsin
    J H McDowell
    Departments of Ophthalmology, University of Florida, Gainesville 32610, USA
    Invest Ophthalmol Vis Sci 42:1439-43. 2001
    ..To determine whether substitution of the potential phosphorylation sites of bovine rhodopsin's carboxyl-terminal region with the acidic residues aspartic acid, glutamic acid, or cysteic acid promotes the activation of arrestin...
  68. ncbi Regulation of rhodopsin dephosphorylation by arrestin
    K Palczewski
    Department of Ophthalmology, University of Florida, Gainesville 32610 0284
    J Biol Chem 264:15770-3. 1989
    We have characterized the opsin phosphatase activities in extracts of rod outer segments and determined their relationship to known protein phosphatases...
  69. pmc Distinctive subtypes of bovine phospholipase C that have preferential expression in the retina and high homology to the norpA gene product of Drosophila
    P A Ferreira
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907
    Proc Natl Acad Sci U S A 90:6042-6. 1993
    ..vertebrate photoreceptors, although light-activated phospholipase C activity has been reported in vertebrate rod outer segments. Conserved regions of norpA cDNA were used to isolate bovine cDNAs that would encode four alternative forms ..
  70. pmc Onset of feedback reactions underlying vertebrate rod photoreceptor light adaptation
    P D Calvert
    Howe Laboratory of Ophthalmology, Harvard Medical School and the Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
    J Gen Physiol 111:39-51. 1998
    ..in Ca2+ and cGMP are followed by the changes in the rates of corresponding enzymatic reactions in frog rod outer segments. Our data indicate that there is a temporal hierarchy among reactions that underlie light adaptation...
  71. ncbi Iris pigment epithelial cell transplantation for degenerative retinal diseases
    Toshiaki Abe
    Division of Clinical Cell Therapy, Center for Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Sendai, Japan
    Prog Retin Eye Res 26:302-21. 2007
    ..Autologous IPE cells have been found in the transplanted subretinal space and were able to phagocytose rod outer segments even 6 months after transplantation...
  72. ncbi Lactose promotes organized photoreceptor outer segment assembly and preserves expression of photoreceptor proteins in retinal degeneration
    M M Jablonski
    Retinal Degeneration Research Center, Department of Ophthalmology, The University of Tennessee Memphis, Memphis, TN 38163, USA
    Mol Vis 5:16. 1999
    ..The purpose of this study was to determine if the difference between organized and disorganized membranes was a variation in the amounts of two structural proteins, opsin and rds/peripherin...
  73. ncbi Identification of the sodium-calcium exchanger as the major ricin-binding glycoprotein of bovine rod outer segments and its localization to the plasma membrane
    D M Reid
    Department of Biochemistry, University of British Columbia, Vancouver, Canada
    Biochemistry 29:1601-7. 1990
    After neuraminidase treatment the Na+/Ca2+ exchanger of bovine rod outer segments was found to specifically bind Ricinus communis agglutinin...
  74. ncbi Association of kinesin with microtubules in diverse cytoskeletal systems in the outer segments of rods and cones
    M S Eckmiller
    C and O Vogt Brain Research Institute, Heinrich Heine University of Düsseldorf School of Medicine, Dusseldorf, Germany
    Acta Anat (Basel) 162:133-41. 1998
    ..occur as the ciliary axoneme in rods and cones, and as a separate cytoskeletal system at the incisures of rod outer segments. We performed an immunocytochemical study of the cytoskeleton in photoreceptors isolated from amphibian ..
  75. ncbi Ca2+ binding capacity of cytoplasmic proteins from rod photoreceptors is mainly due to arrestin
    B Huppertz
    Institut für Biologische Informationsverarbeitung, Forschungszentrum Julich GmbH, Federal Republic of Germany
    J Biol Chem 265:9470-5. 1990
    ..The Ca2+ binding capacity of arresting-containing protein extracts from bovine rod outer segments is about twice as high as that of arrestin-depleted extracts...
  76. ncbi Retinal abnormalities associated with the G90D mutation in opsin
    Muna I Naash
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Comp Neurol 478:149-63. 2004
    ..5) appeared normal for a period of about 3-4 months, but at later ages there were disorganized, shortened rod outer segments (ROS), and a loss of photoreceptor nuclei...
  77. ncbi Signaling properties of a short-wave cone visual pigment and its role in phototransduction
    Guang Shi
    Zilkha Neurogenetic Institute and Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Neurosci 27:10084-93. 2007
    ..The transgenic S-opsin was targeted to rod outer segments, and formed a pigment with peak absorption at 360 nm...
  78. ncbi Trafficking of molecules and metabolic signals in the retina
    M Tsacopoulos
    Department of Physiology, University of Geneva Medical School, Switzerland
    Prog Retin Eye Res 17:429-42. 1998
    ..In rod outer segments (ROS), all-trans-retinal is transformed to all-trans-retinol by retinol dehydrogenase using NADPH...
  79. ncbi Proof of oligomeric state of frog rhodopsin: visualization of dimer and oligomers on gels after BN- and HRCN-PAGE using antibodies to rhodopsin and by retinylopsin fluorescence
    S A Shukolyukov
    Sechenov Institute for Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223, Russia
    Biochemistry (Mosc) 75:1045-51. 2010
    ..Formation of RO in rod outer segments (ROS) in the presence of 0.7 M NaBH(3)CN at pH 5...
  80. ncbi Retinal and brain accretion of long-chain polyunsaturated fatty acids in developing felines: the effects of corn oil-based maternal diets
    R J Pawlosky
    Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcoholism and Alcohol Abuse, Rockville, MD 20852, USA
    Am J Clin Nutr 65:465-72. 1997
    ..In the rod outer segments and brains of these animals, there were lower amounts of 22:6n-3 and higher amounts of long-chain n-6 ..
  81. ncbi Phagosome number and distribution in retinal pigment epithelial cells of vitiligo mutant mice
    B Kosaras
    New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    Exp Eye Res 63:151-8. 1996
    ..Failure of RPE microvilli to intertwine with rod outer segments (ROS) at any age, the accumulation of ROS membranous fragments in the subretinal space, and a relatively ..
  82. ncbi Molecular, enzymatic and functional properties of rhodopsin kinase from rat pineal gland
    K Palczewski
    Department of Ophthalmology, University of Florida, Gainesville 32610 0284
    Vision Res 30:1129-37. 1990
    ..Rhodopsin kinase activity from pineal binds in a light-dependent manner to rhodopsin in rod outer segments as does the enzyme from retina...
  83. ncbi Abnormal cone synapses in human cone-rod dystrophy
    K Gregory Evans
    Department of Ophthalmology, University of Washington, Seattle, USA
    Ophthalmology 105:2306-12. 1998
    ..were decreased in the case of CRD, particularly in the central and far peripheral retina, and both cone and rod outer segments were slightly shortened...
  84. ncbi Constitutive "light" adaptation in rods from G90D rhodopsin: a mechanism for human congenital nightblindness without rod cell loss
    P A Sieving
    Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, Michigan 48105, USA
    J Neurosci 21:5449-60. 2001
    ..G+/+, R-/-) retinas that express only the mutant rhodopsin develop normal rod outer segments and show minimal rod cell loss even at 1 year of age...
  85. ncbi Tyrosine phosphorylation of the alpha subunit of transducin and its association with Src in photoreceptor rod outer segments
    M W Bell
    Department of Biochemistry, Baylor College of Medicine, Houston, Texas, USA
    J Neurochem 75:2006-19. 2000
    Recent evidence indicates that tyrosine phosphorylation may play important roles in retinal photoreceptor rod outer segments (ROS). We investigated the tyrosine phosphorylation of endogenous proteins in isolated bovine ROS...
  86. pmc Evidence for two functionally different membrane fractions in bovine retinal rod outer segments
    P J Bauer
    Institut für Biologische Informationsverarbeitung, Julich, F R G
    J Physiol 401:309-27. 1988
    ..Bovine photoreceptor membrane vesicles were prepared by osmotic lysis and hypotonic wash of purified rod outer segments. 2...
  87. pmc Syntaxin 3 and SNAP-25 pairing, regulated by omega-3 docosahexaenoic acid, controls the delivery of rhodopsin for the biogenesis of cilia-derived sensory organelles, the rod outer segments
    Jana Mazelova
    Department of Surgery, Division of Ophthalmology, University of New Mexico, Albuquerque, New Mexico 87131, USA
    J Cell Sci 122:2003-13. 2009
    The biogenesis of cilia-derived sensory organelles, the photoreceptor rod outer segments (ROS), is mediated by rhodopsin transport carriers (RTCs)...
  88. ncbi Functional abnormalities in transgenic mice expressing a mutant rhodopsin gene
    Y Goto
    Hines Veterans Administration Hospital
    Invest Ophthalmol Vis Sci 36:62-71. 1995
    ..Experimental conditions were chosen to provide a basis of comparison to the results reported for patients with autosomal dominant retinitis pigmentosa (ADRP) in whom the proline at position 23 has been replaced by a histidine (P23H)...
  89. ncbi Delayed rhodopsin regeneration and altered distribution of interphotoreceptor retinoid binding protein (IRBP) in the mi(vit)/mi(vit) (vitiligo) mouse
    S B Smith
    Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, USA
    J Neurocytol 26:605-13. 1997
    Rhodopsin regeneration requires attachment between the retinal pigment epithelium (RPE) and rod outer segments; however, in experimentally induced retinal detachment, rhodopsin regeneration can be restored partially upon addition of IRBP (..
  90. ncbi Recoverin and rhodopsin kinase activity in detergent-resistant membrane rafts from rod outer segments
    Ivan I Senin
    A N Belozersky Institute of Physico Chemical Biology, M V Lomonosov Moscow State University, 119992 Moscow, Russia
    J Biol Chem 279:48647-53. 2004
    Cholesterol-rich membranes or detergent-resistant membranes (DRMs) have recently been isolated from bovine rod outer segments and were shown to contain several signaling proteins such as, for example, transducin and its effector, cGMP-..
  91. ncbi [The alteration of cyclic adenosine monophosphate and protein kinase C in the phagocytic process of human pigment epithelial cells]
    Yu Zhao Sun
    Department of Ophthalmology, the First Clinical College of China Medical University, Shenyang 110001, China
    Zhonghua Yan Ke Za Zhi 40:178-82. 2004
    ....
  92. ncbi Disk membrane initiation and insertion are not required for axial disk displacement in Xenopus laevis rod outer segments
    M W Kaplan
    R S Dow Neurological Sciences Institute, Portland, OR 97209 1595, USA
    Curr Eye Res 17:73-8. 1998
    ..This study was designed to determine whether the axial displacement of disk membranes requires the assembly and insertion of new disk lamellae...
  93. ncbi Melanin protects choroidal blood vessels against light toxicity
    Swaantje Peters
    Center of Ophthalmology, University of Tuebingen, Schleichstr 12, D 72076 Tuebingen, Germany
    Z Naturforsch C 61:427-33. 2006
    ..In albino rats severe retinal damage was observed after light exposure, rod outer segments (ROS) were shortened and the thickness of the outer nuclear layer (ONL) was significantly diminished...
  94. ncbi Rod phototransduction in transgenic mice expressing a mutant opsin gene
    Y Goto
    Hines Veterans Administration Hospital, Illinois 60141, USA
    J Opt Soc Am A Opt Image Sci Vis 13:577-85. 1996
    ..This decrease was consistent with reductions in the number of rod photoreceptors and in the length of rod outer segments observed in previous histological studies of the VPP retina...
  95. pmc Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin
    A E Otto Bruc
    Department of Ophthalmology, University of Washington, Seattle, WA 98195 6485, USA
    Proc Natl Acad Sci U S A 95:15014-9. 1998
    ..Pieces of bovine retina with intact rod outer segments were treated with pore-forming staphylococcal alpha-toxin, including an alpha-toxin mutant that forms pores ..
  96. ncbi Mass spectrometric analysis of rhodopsin from light damaged rats
    Z Ablonczy
    Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 6:109-15. 2000
    ..Rhodopsin has been proposed to be involved in this process. We therefore undertook to examine whether rhodopsin isolated from light damaged animals is structurally altered at the molecular level...
  97. ncbi Activation of the rod G-protein Gt by the thrombin receptor (PAR1) expressed in Sf9 cells
    C Seibert
    Institut fur Medizinische Physik und Biophysik, Medizinische Fakultät Charité, Humboldt Universitat, Berlin, Germany
    Eur J Biochem 266:911-6. 1999
    ..system of membranes from Sf9 cells expressing the thrombin receptor and purified Gt from bovine rod outer segments. TRAP6-agonist-activated PAR1 interacts productively with the distant G-protein...
  98. ncbi Transducin activation state controls its light-dependent translocation in rod photoreceptors
    Vasily Kerov
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Biol Chem 280:41069-76. 2005
    Light-dependent redistribution of transducin between the rod outer segments (OS) and other photoreceptor compartments including the inner segments (IS) and synaptic terminals (ST) is recognized as a critical contributing factor to light ..
  99. pmc Physiological properties of rod photoreceptor cells in green-sensitive cone pigment knock-in mice
    Keisuke Sakurai
    Department of Biophysics, Graduate School of Science, Kyoto University and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kyoto 606 8502, Japan
    J Gen Physiol 130:21-40. 2007
    ..The mouse green was successfully transported to the rod outer segments, though the expression of mouse green in homozygous retina was approximately 11% of rhodopsin in wild-type ..
  100. ncbi Pigment epithelial and retinal phenotypes in the vitiligo mivit, mutant mouse
    R L Sidman
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    Invest Ophthalmol Vis Sci 37:1097-115. 1996
    ..To describe the abnormal phenotype in retinal pigment epithelium (RPE) and neural retina of vitiligo mutant mice from embryonic stages to old age...
  101. ncbi Delayed expression of the Crx gene and photoreceptor development in the Chx10-deficient retina
    Adam D Rutherford
    Developmental Biology Unit, Institute of Child Health, University College London, London, United Kingdom
    Invest Ophthalmol Vis Sci 45:375-84. 2004
    ..The purpose of this study was to examine how neuronal development is affected by absence of the Chx10 transcription factor in the mouse retina...

Research Grants62

  1. SECOND MESSENGERS IN THE RETINA
    RAJU VS RAJALA; Fiscal Year: 2012
    ..Studies from our laboratory over the past two decades have shown that the retina and rod outer segments (ROS) have an active PI metabolism...
  2. MEMBRANE FUSION IN RETINAL ROD OUTER SEGMENTS
    KATHLEEN BOESZEBATTAGLIA; Fiscal Year: 2012
    ..Our studies should reveal novel insights into the pathogenesis of peripherin/rds mediated retinal dystrophies and form the underpinnings for the development of therapeutic strategies to treat these diseases. ..
  3. Illuminating the process of rod outer segment morphogenesis
    Yoshikazu Imanishi; Fiscal Year: 2013
    ..is essential for phototransduction signaling, as well as the morphogenesis and maintenance of the rod outer segments. Since photoreceptor outer segments do not have machinery for protein synthesis, proteins need to be ..
  4. Assembly and Trafficking of Heterotrimeric G Proteins in Vertebrate Photoreceptor
    Maxim Sokolov; Fiscal Year: 2013
    ..we will determine in vivo the significance of each site in regulating trafficking of transducin to the rod outer segments. The proposed studies will reveal the function of the eukaryotic chaperonin, CCT, in vertebrate ..
  5. Genetic Mechanisms of Retinal Degenerative Disease
    JANIS LEM GEE; Fiscal Year: 2010
    ..retinal degeneration associated with elevated cGMP in the "rd" mouse retina is caused by excessive Ca2+ in rod outer segments and can be ameliorated by reducing intracellular Ca2+...
  6. RHODOPSIN TRAFFICKING AND PHOTORECEPTOR MEMBRANE RENEWAL
    DUSANKA DANIELLE DERETIC; Fiscal Year: 2013
    ..ciliary membrane proteins, some of which bind Arf4, this leads us to propose that membrane targeting to the rod outer segments (ROS) is equivalent to ciliary targeting, and that the complex we identified is a ciliary targeting complex...
  7. Trasnducin Interactions with Photoreceptor Membranes
    Theodore G Wensel; Fiscal Year: 2013
    ..most informative model systems for developing this understanding is the phototransduction pathway of retinal rod outer segments, in which a light signal, photon capture by the G-protein coupled receptor (GPCR), rhodopsin, is converted ..
  8. Photoreceptor Cell Vulnerability and Glutathione Status
    Barry S Winkler; Fiscal Year: 2010
    ..We will evaluate this hypothesis by using whole rat retinas, serial sections of rat retinas, and bovine rod outer segments in electrophysiological and metabolic experiments designed to assess the linkage between photoreceptor cell ..
  9. Use of Xenopus a system to study the trafficking,function and structure of IRBP
    Federico Gonzalez-Fernandez; Fiscal Year: 2013
    ..As the major soluble component of the interphotoreceptor matrix (IPM), has access to the cone and rod outer segments, apical RPE surface, and M[unreadable]ller cell villi, The mechanism for IRBP's complex function in ..
  10. Conferene on the basic cell and molecular biology of cilia and flagella as relate
    Joel Rosenbaum; Fiscal Year: 2007
    ..example is Retinitis Pigmentosa; although it has been known for decades that a cilium gives rise to the rod outer segments of the retina, it has only been within the past few years that the transport processes occurring within this ..
  11. Adaptive optics imaging of geographic atrophy progression in macular degeneration
    MINA MILLICENT CHUNG; Fiscal Year: 2013
    ..Test the hypothesis that loss of rod photoreceptors in GA is preceded by visual cycle disruption in the rod outer segments. Expected outcomes of the proposed study include determination of the primary cell type affected in the GA ..
  12. DIETARY N-3 FATTY ACIDS: TRANSPORT AND UPTAKE INTO BRAIN
    Gregory Anderson; Fiscal Year: 1993
    ..The brain and retina, and in particular the synaptic membranes and rod outer segments, are especially rich in the n-3 family of polyunsaturated fatty s...
  13. PROTEIN KINASE C AND PHOTORECEPTORS
    ALEXANDRA NEWTON; Fiscal Year: 2002
    The long term goal of the proposed research if to elucidate the role of protein kinase C in rod outer segments of photoreceptors...
  14. CONFORMATIONAL ASPECTS OF VISUAL EXCITATION
    LUBERT STRYER; Fiscal Year: 1993
    ..electrophysiological studies of rod outer segment proteins: (1) The light-triggered amplification cycle in rod outer segments involving photoexcited rhodopsin, transducin, and the cyclic GMP phosphodiesterase (PDE) will be ..
  15. ROLE OF SUGARS IN PHAGOCYTOSIS OF ROD OUTER SEGMENTS
    EDWARD KEAN; Fiscal Year: 1991
    ..cells of the embryonic chick maintained in cell culture as the biological systems, and rhodopsin-liposomes, rod outer segments (ROS), and disc membranes prepared from the ROS as the target rhodopsin- containing membranes...
  16. PHOTORECEPTOR GENE EXPRESSION DURING DEVELOPMENT
    William Hauswirth; Fiscal Year: 1999
    ..induced simultaneously at about 6 months (2/3) gestation, corresponding to the initial emergence of rod outer segments, (2) the rod opsin gene experiences specific transcriptional enhancement not seen for other ..
  17. STUDIES ON RHODOPSIN & AGE-RELATED MACULAR DEGENERATION
    Koji Nakanishi; Fiscal Year: 2006
    ..of A2E) to damage DNA by binding covalently to nucleic acids; vi) chemical and cellular studies of the all-trans-retinal dimer, a fluorophore newly isolated from rod outer segments, the same source as the A2E pigments.
  18. GUANYLYL CYCLASE ACTIVATING PROTEIN AND OLFACTION
    Cheil Moon; Fiscal Year: 2000
    ..In low Ca/2+, GCAP is activated to enhance GC and restore cGMP levels in rod outer segments. Our preliminary studies demonstrate that GCAP is expressed in olfactory receptor neurons (ORNs) and ..
  19. MOLECULAR MECHANISMS OF RPE PHAGOCYTOSIS--ROLE OF CD36
    Roy Silverstein; Fiscal Year: 2002
    ..studies to be expressed on RPE in a developmentally regulated manner, and to function as a receptor for rod outer segments (ROS)...
  20. PHOTORECEPTOR-AND RETINAL SYNAPSE PHYSIOLOGY
    PETER MACLEISH; Fiscal Year: 1993
    ..For rods, the effect on the light response of injecting antibodies directed against rod outer segments will be examined to generate specific reagents that can be used in analyzing the biochemical steps in the ..
  21. TRANSDUCTION IN VERTEBRATE PHOTORECEPTORS
    Shaul Hestrin; Fiscal Year: 1991
    ..3. Determination of the specific effects of cytoplasmic calcium on guanylate cyclase in detached rod outer segments. Experimental manipulations that change the concentration of cytoplasmic calcium in rods affect the ..
  22. MOLECULAR PROPERTIES OF A CGMP-ACTIVATED CHANNEL
    STANLEY GOLDIN; Fiscal Year: 1991
    ..Our laboratory has developed a procedure for purification of discs from bovine rod outer segments, and demonstrated that such a channel is associated with discs...
  23. DEVELOPMENT AND MAINTENANCE OF RETINAL CELLS
    Joe Hollyfield; Fiscal Year: 1991
    ..phosphorylation reactions, and calcium fluxes in establishing the burst of membrane assembly at the base of rod outer segments which follows the onset of the light cycle...
  24. PROTEIN MODIFICATION IN AGE-RELATED RETINAL DEGENERATION
    DEBORAH FERRINGTON; Fiscal Year: 2001
    ..Proteasome function and the extent of oxidative damage to proteins will be investigated in rod outer segments (ROS) and the retinal pigment epithelium (RPE) of the retinas from Fisher 344 rats (10, 23, 26 months)...
  25. BIOPHYSICAL STUDY OF ORIENTED PHOTORECEPTOR MEMBRANE
    Kenneth Rothschild; Fiscal Year: 1980
    ..based on liquid-crystal physics are being developed to orient photoreceptor membrane isolated from retinal rod outer segments into liquid-crystal films...
  26. DETERMINANTS OF RETINAL PHOTORECEPTOR MORPHOLOGY
    JOSEPH CORLESS; Fiscal Year: 1991
    ..from or remain connected to the plasmalemma? Our recent morphological studies of the disk margin of frog rod outer segments (ROS) have revealed a clustering of structures we term the terminal loop (TL)-complex, to emphasize their ..
  27. BIOCHEMICAL STUDIES OF ROD OUTER SEGMENT PLASMA MEMBRANE
    Arthur S Polans; Fiscal Year: 1991
    ..The first involves probing the extracellular surface of intact rod outer segments with antibodies and lectins already available...
  28. Protein Nitration in Retinal Light Damage
    Masaru Miyagi; Fiscal Year: 2004
    ..in protein expression levels and proteins which are nitrated by night exposure in whole rat retina and rod outer segments, and 3) Characterize structural changes in LEDGF induced by light exposure...
  29. Retinal Degeneration Conference
    Robert Anderson; Fiscal Year: 2008
    ..In this proposal, we request funds to provide: 1) full travel awards to 20 young investigators and 3 keynote speakers and 2) some administrative expenses. [unreadable] [unreadable] [unreadable]..
  30. Conference--Retinal Degeneration
    Robert Anderson; Fiscal Year: 2002
    ..This is a key feature that distinguishes the International Symposium on Retinal Degenerations from other scientific meetings. ..
  31. Retinal Degeneration Conference
    Robert Anderson; Fiscal Year: 2006
    ..In this competitive renewal, we request funds to provide: 1) full travel awards to 20 young investigators and 3 keynote speakers and 2) some administrative expenses. [unreadable] [unreadable] [unreadable] [unreadable]..
  32. Gas6/Mertk mediated phagocytosis of OS by RPE cells
    Michael Hall; Fiscal Year: 2005
    ..abstract_text> ..
  33. Retinal Degeneration Conference
    Robert Anderson; Fiscal Year: 2004
    ..In this competitive renewal, we request funds to provide: 1) full travel awards to 25 young investigators and 1 keynote speakers, 2) some administrative expenses, and 3) modest support for all scientists who participate. ..
  34. Light Adaptation in Vertebrate Rod Photoreceptors
    CLINT MAKINO; Fiscal Year: 2005
    ..Aberrant responses occur with higher frequency in some human retinal diseases and severely limit the intensity range of scotopic vision. ..
  35. ENVIRONMENTAL LIGHT AND RETINAL MEMBRANE DEVELOPMENT
    Daniel Organisciak; Fiscal Year: 2003
    ..In addition, the time course of the appearance of cytoplasmic cytochrome C and the activation of cellular caspases will be determined by antibody-based techniques, immunocytochemistry and enzymatic analysis. ..
  36. UW Oshkosh Animal Facility Improvement
    DANA VAUGHAN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  37. Sustained, Non-viral Ocular Therapy Using Nanoparticles
    Muna Naash; Fiscal Year: 2006
    ..When taken together, these studies will provide valuable insight into non-viral gene therapy as a prospective method for the treatment of inherited blinding diseases. [unreadable] [unreadable] [unreadable]..
  38. Prevention of Inherited Retinal Diseases by Therapeutic Rare Earth Nanoparticles
    JAMES FRANCIS MCGINNIS; Fiscal Year: 2010
    ..The nanoceria are expected to function in humans as they do in other mammals and therefore should preserve vision and prevent blindness in humans. ..
  39. STRUCTURAL STUDIES OF G PROTEIN-COUPLED RECEPTORS
    KRZYSZTOF NMI PALCZEWSKI; Fiscal Year: 2010
    ..The importance of the protein family for human health makes this effort worthwhile. ..
  40. NEURONAL DEGENERATION HEREDITARY CEROID-LIPOFUSCINOSIS
    Martin Katz; Fiscal Year: 2003
    ....
  41. Modes of Neurotoxicity in the Mammalian Retina
    DENNIS GOEBEL; Fiscal Year: 2006
    ..abstract_text> ..
  42. ROD NEURODEGENERATIVE AND PROTECTIVE GENES
    Baerbel Rohrer; Fiscal Year: 2005
    ..We expect to isolate common downstream target genes that are activated both during degeneration and neuroprotection. ..
  43. Conference--The Biology and Chemistry of Vision
    Wolfgang Baehr; Fiscal Year: 2003
    ..Proposed sessions include topics on the biology and physiology of photoreceptors, human genetics and retinal disease, as well as gene therapy of recessive and dominant dystrophies. ..
  44. p27Kip1 and RPE Cell Cycle
    DENNIS DEFOE; Fiscal Year: 2003
    ..The alternative hypothesis that the thickening of the RPE layer is due to an increase in cell size will be examined by morphometric analysis of images obtained by confocal microscopy. ..
  45. Acquistion of a 600MHz NMR Spectrometer
    ARLENE ALBERT; Fiscal Year: 2003
    ..These instruments lack the resolution, sensitivity and available time to run the projects described above. These researchers currently travel a minimum of an hour to acquire the data needed on several funded projects. ..
  46. OLYMPUS FLUOVIEW CONFOCAL LASER SCANNING MICROSCOPE
    Steven Fisher; Fiscal Year: 2003
    ..The enhanced multi-labeling capabilities and capacity to support living cell studies will allow investigators to explore novel research avenues that are unapproachable with our current instrumentation. ..
  47. ANTIBODY-MEDIATED DEATH OF MAMMALIAN PHOTORECEPTOR CELLS
    James McGinnis; Fiscal Year: 2003
    ..The data from these studies may have direct applicability to the prevention of blindness in CAR as well as to the rescue of neurons in other paraneoplastic neuropathies. ..
  48. Alpha v beta 5 integrin in retinal pigment epithelium
    SILVIA FINNEMANN; Fiscal Year: 2004
    ..The mechanisms of these interactions and the unique localization of v5 integrin in RPE are conserved among species. Exploring the 5 null mouse is thus important to understand human disease. ..
  49. Mechanism of photoreceptor cell degeneration in animal models of retinal diseases
    Muna Naash; Fiscal Year: 2008
    ..cone OSs morphogenesis and maintenance. Experiments put forth in this application will provide biochemical and physiological evidence for the role of Rds and its associated proteins during genesis of rod and cone OSs. ..
  50. B-CLL Proteins Quantified by Mass Spectrometry
    DAVID BARNIDGE; Fiscal Year: 2004
    ..As part of this proposal the candidate will take courses offered in the Mayo Graduate School on Techniques in Molecular Biology, Human Cytogenetics, and Immunology. ..
  51. The Integrin Ligand MFG-E8 and Photoreceptor Outer Segment Renewal.
    SILVIA FINNEMANN; Fiscal Year: 2007
    ....
  52. TYROSINE PHOSPHORYLATION AND MAMMALIAN RETINA
    ABBOUD GHALAYINI; Fiscal Year: 2001
    ..in vivo show that: 1) light stimulates tyrosine phosphorylation of photoreceptor out segment proteins, 2) rod outer segments (ROS) from light-adapted retinas are enriched with the tyrosine kinase c-Src,PLCgamma/1, and arresting, 3) ..
  53. Experimental Retinal Detachment and Reattachment
    Steven Fisher; Fiscal Year: 2008
    ..5) To develop a mouse model of detachment and to study mice deficient in the two intermediate filament proteins, GFAP and vimentin to determine their roles in glial cell responsiveness to detachment. [unreadable] [unreadable]..
  54. IMAGING OF ROD OUTER SEGMENT BY CRYO-ELECTRON TOMOGRAPHY
    Krzysztof Palczewski; Fiscal Year: 2008
    ..In this study, we propose to develop cryo-electron tomography (cryo-ET) to study the structure of rod outer segments (ROS)...
  55. p27(Kip1) and Retinal Attachment
    DENNIS DEFOE; Fiscal Year: 2008
    ..To test this, the Cre-lox system for conditional gene targeting will be implemented to ablate the p27(Kip1) gene selectively in RPE cells. [unreadable] [unreadable] [unreadable]..
  56. Structural Analysis of the Rhodopsin-Transducin Complex
    KEVIN RIDGE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  57. CYCLIC GMP SIGNALING PATHWAYS IN RETINA
    SITARAMAYYA ARI; Fiscal Year: 2003
    ..Successful completion of the proposed studies will, for the first time, establish the biochemical mechanism of cyclic GMP action in light adaptation, and identify the proteins that mediate its effects. ..
  58. TRANSMISSION ELECTRON MICROSCOPE
    Martin Katz; Fiscal Year: 2006
    ..Broadening the user base will help make the cost per user of maintaining the instrument as low as possible. [unreadable] [unreadable]..
  59. STRUCTURAL STUDIES OF G-PROTEIN COUPLED RECEPTORS
    KEVIN RIDGE; Fiscal Year: 2004
    ....