Summary: The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.

Top Publications

  1. ncbi Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
    Jeongwu Lee
    Neuro Oncology Branch, National Cancer Institute, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Cell 9:391-403. 2006
  2. pmc Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR)
    Evan M Kroh
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Methods 50:298-301. 2010
  3. pmc Genome-wide association study identifies multiple loci influencing human serum metabolite levels
    Johannes Kettunen
    Institute for Molecular Medicine Finland, University of Helsinki, Finland
    Nat Genet 44:269-76. 2012
  4. doi HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software
    Louise Royle
    Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
    Anal Biochem 376:1-12. 2008
  5. pmc Identification of serum metabolites associated with risk of type 2 diabetes using a targeted metabolomic approach
    Anna Floegel
    Department of Epidemiology, German Institute of Human Nutrition Potsdam Rehbruecke, Nuthetal, Germany
    Diabetes 62:639-48. 2013
  6. pmc Comparing the MicroRNA spectrum between serum and plasma
    Kai Wang
    Institute for Systems Biology, Seattle, Washington, United States of America
    PLoS ONE 7:e41561. 2012
  7. pmc A serum circulating miRNA diagnostic test to identify asymptomatic high-risk individuals with early stage lung cancer
    Fabrizio Bianchi
    IFOM, the FIRC Institute for Molecular Oncology Foundation, at the IFOM IEO Campus, Milan, Italy
    EMBO Mol Med 3:495-503. 2011
  8. doi Assessing sample and miRNA profile quality in serum and plasma or other biofluids
    Thorarinn Blondal
    Exiqon A S, Skelstedet 16, 2950 Vedbæk, Denmark
    Methods 59:S1-6. 2013
  9. doi Quantitative assessment of human serum high-abundance protein depletion
    Rene Stempfer
    Molecular Diagnostics, Life Sciences, Austrian Research Centers GmbH ARC, Seibersdorf, Austria
    Electrophoresis 29:4316-23. 2008
  10. doi Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Heidelberg, Germany
    Stem Cells 27:2331-41. 2009

Detail Information

Publications421 found, 100 shown here

  1. ncbi Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines
    Jeongwu Lee
    Neuro Oncology Branch, National Cancer Institute, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cancer Cell 9:391-403. 2006
    ..These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors...
  2. pmc Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR)
    Evan M Kroh
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Methods 50:298-301. 2010
    ..circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases...
  3. pmc Genome-wide association study identifies multiple loci influencing human serum metabolite levels
    Johannes Kettunen
    Institute for Molecular Medicine Finland, University of Helsinki, Finland
    Nat Genet 44:269-76. 2012
    ..7 million SNPs for a range of 216 serum metabolic phenotypes assessed by NMR of serum samples. We identified significant associations (P < 2...
  4. doi HPLC-based analysis of serum N-glycans on a 96-well plate platform with dedicated database software
    Louise Royle
    Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK
    Anal Biochem 376:1-12. 2008
    ..that accesses our database (GlycoBase) of more than 350 N-glycan structures, including 117 present in the human serum glycome; and (iv) software (autoGU) that progressively analyzes data from exoglycosidase digestions to produce a ..
  5. pmc Identification of serum metabolites associated with risk of type 2 diabetes using a targeted metabolomic approach
    Anna Floegel
    Department of Epidemiology, German Institute of Human Nutrition Potsdam Rehbruecke, Nuthetal, Germany
    Diabetes 62:639-48. 2013
    ..We prospectively investigated the association between serum metabolites measured by targeted metabolomics and risk of T2D in the European Prospective Investigation into ..
  6. pmc Comparing the MicroRNA spectrum between serum and plasma
    Kai Wang
    Institute for Systems Biology, Seattle, Washington, United States of America
    PLoS ONE 7:e41561. 2012
    ..In this study we focused on different sample types' effects on the spectrum of circulating miRNA in blood. Using serum and corresponding plasma samples from the same individuals, we observed higher miRNA concentrations in serum ..
  7. pmc A serum circulating miRNA diagnostic test to identify asymptomatic high-risk individuals with early stage lung cancer
    Fabrizio Bianchi
    IFOM, the FIRC Institute for Molecular Oncology Foundation, at the IFOM IEO Campus, Milan, Italy
    EMBO Mol Med 3:495-503. 2011
    ..We developed a test, based on the detection of 34 microRNAs (miRNAs) from serum, that could identify patients with early stage non-small cell lung carcinomas (NSCLCs) in a population of ..
  8. doi Assessing sample and miRNA profile quality in serum and plasma or other biofluids
    Thorarinn Blondal
    Exiqon A S, Skelstedet 16, 2950 Vedbæk, Denmark
    Methods 59:S1-6. 2013
    ..The high relative stability of miRNA in common clinical tissues and biofluids (e.g. plasma, serum, urine, saliva, etc...
  9. doi Quantitative assessment of human serum high-abundance protein depletion
    Rene Stempfer
    Molecular Diagnostics, Life Sciences, Austrian Research Centers GmbH ARC, Seibersdorf, Austria
    Electrophoresis 29:4316-23. 2008
    The aim of this study is to quantify the effectivity of the depletion of human high-abundance serum and plasma proteins for improved protein identification and disease marker candidate discovery and to assess the risk of concomitant ..
  10. doi Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Heidelberg, Germany
    Stem Cells 27:2331-41. 2009
    ..For clinical scale manufacturing, human factors from serum or platelets have been suggested as alternatives to fetal bovine serum (FBS)...
  11. doi High-throughput serum NMR metabonomics for cost-effective holistic studies on systemic metabolism
    Pasi Soininen
    NMR Metabonomics Laboratory, Laboratory of Chemistry, Department of Biosciences, University of Kuopio, Kuopio, Finland
    Analyst 134:1781-5. 2009
    ..two molecular windows that contain the majority of the metabolic information available by 1H NMR from native serum, e.g. serum lipids, lipoprotein subclasses as well as various low-molecular-weight metabolites...
  12. doi Mechanism of Trypanosoma brucei gambiense resistance to human serum
    Pierrick Uzureau
    Laboratory of Molecular Parasitology, IBMM, Universite Libre de Bruxelles, 12 rue des Prof Jeener et Brachet, B 6041 Gosselies, Belgium
    Nature 501:430-4. 2013
    ..Human immunity to some African trypanosomes is due to two serum complexes designated trypanolytic factors (TLF-1 and -2), which both contain haptoglobin-related protein (HPR) and ..
  13. pmc Value of soluble TREM-1, procalcitonin, and C-reactive protein serum levels as biomarkers for detecting bacteremia among sepsis patients with new fever in intensive care units: a prospective cohort study
    Longxiang Su
    Department of Respiratory Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya, Hainan Province 572013, China
    BMC Infect Dis 12:157. 2012
    ..triggering receptor expressed on myeloid cells 1 (sTREM-1), procalcitonin (PCT), and C-reactive protein (CRP) serum levels for differentiating sepsis from SIRS, identifying new fever caused by bacteremia, and assessing prognosis ..
  14. pmc Haptoglobin-hemoglobin receptor independent killing of African trypanosomes by human serum and trypanosome lytic factors
    Whitney Bullard
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA
    Virulence 3:72-6. 2012
    ..We now show that loss of the TbbHpHbR reduces the susceptibility of T. b. brucei to human serum and TLF-1 by 100- and 10,000-fold, respectively...
  15. doi Elevated serum levels of high mobility group box 1 (HMGB1) protein in dengue-infected patients are associated with disease symptoms and secondary infection
    Diego Allonso
    Laboratory of Structural Genomics, Biophysics Institute Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil
    J Clin Virol 55:214-9. 2012
    ..Recently, it was observed that both DENV-infected epithelial cells undergoing necrosis and dendritic cells (DCs) are able to release a non-classical pro-inflammatory cytokine called high mobility group box 1 (HMGB1)...
  16. doi Expression profile of microRNAs in serum: a fingerprint for esophageal squamous cell carcinoma
    Chunni Zhang
    Department of Clinical Laboratory, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing, China
    Clin Chem 56:1871-9. 2010
    ..The discovery of serum microRNAs (miRNAs) and their unique concentration profiles in patients with various diseases makes them attractive,..
  17. pmc Antigen microarrays identify unique serum autoantibody signatures in clinical and pathologic subtypes of multiple sclerosis
    Francisco J Quintana
    Center for Neurologic Diseases, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:18889-94. 2008
    ..We performed antigen microarray analysis to characterize patterns of antibody reactivity in MS serum against a panel of CNS protein and lipid autoantigens and heat shock proteins...
  18. ncbi Standardized approach to proteome profiling of human serum based on magnetic bead separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Sven Baumann
    Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Leipzig, Germany
    Clin Chem 51:973-80. 2005
    ..The aims of our study were to establish a proteome fractionation technique and to validate a standardized blood sampling, processing, and storage procedure for proteomic pattern analysis...
  19. pmc Serum metabolomics reveals higher levels of polyunsaturated fatty acids in lepromatous leprosy: potential markers for susceptibility and pathogenesis
    Reem Al-Mubarak
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America
    PLoS Negl Trop Dis 5:e1303. 2011
    ..Various studies have examined lipid metabolism in leprosy, but there has been limited work using whole metabolite profiles to distinguish the clinical forms of leprosy...
  20. doi Class 2 IGF-1 isoforms are dispensable for viability, growth and maintenance of IGF-1 serum levels
    L Temmerman
    European Molecular Biology Laboratory Mouse Biology Unit, Via Ramarini 32, I 00015 Monterotondo Scalo, Roma, Italy
    Growth Horm IGF Res 20:255-63. 2010
    ..not necessary for systemic circulation of IGF-1, revealing an internal compensation system for maintaining IGF-1 serum concentrations...
  21. doi Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods
    J van der Valk
    Fac Veterinary Medicine, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands
    Toxicol In Vitro 24:1053-63. 2010
    ..This will decrease the dependence on animal serum, a supplement with an undefined and variable composition...
  22. pmc Differences between human plasma and serum metabolite profiles
    Zhonghao Yu
    Research Unit of Molecular Epidemiology, Helmholtz Zentrum Munchen, Neuherberg, Germany
    PLoS ONE 6:e21230. 2011
    Human plasma and serum are widely used matrices in clinical and biological studies. However, different collecting procedures and the coagulation cascade influence concentrations of both proteins and metabolites in these matrices...
  23. pmc Hepatitis C virus replication in transfected and serum-infected cultured human fetal hepatocytes
    Catherine A Lazaro
    Department of Pathology, University of Washington School of Medicine, K078 Health Sciences Building, Box 357705, Seattle, WA 98195 7705, USA
    Am J Pathol 170:478-89. 2007
    ..This culture system may be used to study the responses of nontransformed human hepatocytes to HCV infection, to analyze serum infectivity, and to clone novel HCVs from infected patients.
  24. doi The utility of serum C-reactive protein in differentiating bacterial from nonbacterial pneumonia in children: a meta-analysis of 1230 children
    Robert G Flood
    Department of Pediatrics, Temple University School of Medicine, Philadelphia, PA 19140, USA
    Pediatr Infect Dis J 27:95-9. 2008
    ..Although several studies have evaluated serum concentrations of C-reactive protein (CRP) as a predictor of bacterial pneumonia in this patient population, the ..
  25. ncbi Expressing the Modification of Diet in Renal Disease Study equation for estimating glomerular filtration rate with standardized serum creatinine values
    Andrew S Levey
    Division of Nephrology, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 53:766-72. 2007
    We sought to reexpress the 4-variable Modification of Diet in Renal Disease (MDRD) Study equation for estimation of glomerular filtration rate (GFR) using serum creatinine (S(cr)) standardized to reference methods.
  26. pmc Preclinical prophylactic efficacy testing of Sm-p80-based vaccine in a nonhuman primate model of Schistosoma mansoni infection and immunoglobulin G and E responses to Sm-p80 in human serum samples from an area where schistosomiasis is endemic
    Gul Ahmad
    Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA
    J Infect Dis 204:1437-49. 2011
    ..Human correlate studies revealed Sm-p80 reactivity with immunoglobulin G in human serum samples from schistosome-infected individuals...
  27. doi A new quantitative RT-PCR method for sensitive detection of dengue virus in serum samples
    Nadine Sadon
    GlaxoSmithKline Biologicals, Research and Development, Viral Vaccines, Rixensart, Belgium
    J Virol Methods 153:1-6. 2008
    In order to detect and identify dengue serotypes in serum samples, we developed a single-step quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay (referred to as Q-PCR)...
  28. pmc Uremic serum and solutes increase post-vascular interventional thrombotic risk through altered stability of smooth muscle cell tissue factor
    Vipul C Chitalia
    Harvard MIT Division of Science and Technology, Bldg E25 449, Massachusetts Institute of Technology, 77 Mass Ave, Cambridge, MA 02139, USA
    Circulation 127:365-76. 2013
    ..We posit that uremia modulates TF in the local vessel wall to induce postinterventional thrombosis in patients with end-stage renal disease...
  29. ncbi Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue
    Asli Kocaoemer
    Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden Württemberg Hessen, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany
    Stem Cells 25:1270-8. 2007
    ..However, most isolation and expansion protocols for clinical-scale production of MSCs use fetal calf serum (FCS) as a supplement, which poses a potential risk for infections as well as immunological reactions...
  30. doi Serum metabolomics as a novel diagnostic approach for disease: a systematic review
    Aihua Zhang
    National TCM Key Lab of Serum Pharmacochemistry, Key Pharmacometabolomics Platform of Chinese Medicines, and Heilongjiang University of Chinese Medicine, Harbin, China
    Anal Bioanal Chem 404:1239-45. 2012
    ..Monitoring specific metabolite levels in serum, the most commonly used biofluid in metabolomics, has become an important way of detecting the early stages of a ..
  31. pmc Strong association between non alcoholic fatty liver disease (NAFLD) and low 25(OH) vitamin D levels in an adult population with normal serum liver enzymes
    Ilaria Barchetta
    Department of Internal Medicine and Medical Specialties, Sapienza University of Rome, Italy
    BMC Med 9:85. 2011
    ..Aim of our study was to test the hypothesis of a direct association between hypovitaminosis D and the presence of NAFLD in subjects with various degree of insulin-resistance and related metabolic disorders...
  32. doi Low serum iron levels are associated with elevated plasma levels of coagulation factor VIII and pulmonary emboli/deep venous thromboses in replicate cohorts of patients with hereditary haemorrhagic telangiectasia
    John A Livesey
    Imperial College School of Medicine, Imperial College, London, UK
    Thorax 67:328-33. 2012
    ..Elevated plasma levels of coagulation factor VIII are a strong risk factor for pulmonary emboli and deep venous thromboses...
  33. ncbi Serum derived from multiple trauma patients promotes the differentiation of endothelial progenitor cells in vitro: possible role of transforming growth factor-beta1 and vascular endothelial growth factor165
    Dirk Henrich
    Department of Trauma Surgery, Johann Wolfgang Goethe University, Frankfurt Main, Germany
    Shock 21:13-6. 2004
    ..Here, we report that serum derived from patients with MT stimulates differentiation of EPCs in vitro from peripheral blood mononuclear cells (..
  34. pmc Genome-wide mapping of Myc binding and gene regulation in serum-stimulated fibroblasts
    D Perna
    Department of Experimental Oncology, European Institute of Oncology, IFOM IEO Campus, Milan, Italy
    Oncogene 31:1695-709. 2012
    The transition from quiescence to proliferation is a key regulatory step that can be induced by serum stimulation in cultured fibroblasts...
  35. doi Strategies for extended serum half-life of protein therapeutics
    Roland E Kontermann
    Institut fur Zellbiologie und Immunologie, Universitat Stuttgart, Allmandring 31, 70569 Stuttgart, Germany
    Curr Opin Biotechnol 22:868-76. 2011
  36. doi Toxicity of amorphous silica nanoparticles on eukaryotic cell model is determined by particle agglomeration and serum protein adsorption effects
    Daniela Drescher
    BAM Federal Institute for Materials Research and Testing, Richard Willstätter Str 11, 12489 Berlin, Germany
    Anal Bioanal Chem 400:1367-73. 2011
    ..on XTT viability assay, cytotoxicity is shown to be a function of (1) particle concentration and (2) of fetal calf serum (FCS) content in the cell culture medium...
  37. doi Serum bilirubin and genes controlling bilirubin concentrations as biomarkers for cardiovascular disease
    Jing Ping Lin
    Office of Biostatistics Research, Division of Cardiovascular Science, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
    Clin Chem 56:1535-43. 2010
    b>Serum bilirubin has been consistently shown to be inversely related to cardiovascular disease (CVD)...
  38. ncbi LC-MS/MS quantification of Zn-alpha2 glycoprotein: a potential serum biomarker for prostate cancer
    Olga P Bondar
    Department of Laboratory Medicine and Pathology, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA
    Clin Chem 53:673-8. 2007
    ..We present a high-flow liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring serum ZAG concentrations by proteolytic cleavage of the protein and quantification of a unique peptide.
  39. doi Neuroprotective effects of salidroside in the PC12 cell model exposed to hypoglycemia and serum limitation
    Shu Yu
    Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, JS 226001, People s Republic of China
    Cell Mol Neurobiol 28:1067-78. 2008
    The hypoglycemia and serum limitation-induced cell death in cultured PC12 cells represents a useful in vitro model for the study of brain ischemia and neurodegenerative disorders...
  40. doi High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans
    Andrew M Goetze
    Department of Process and Product Development, Amgen Inc, Thousand Oaks, CA 91320, USA
    Glycobiology 21:949-59. 2011
    ..The therapeutic IgGs were affinity purified from serum samples from human pharmacokinetic studies, and changes to the glycan profile over time were determined by peptide ..
  41. pmc Effects of chemical modification on the potency, serum stability, and immunostimulatory properties of short shRNAs
    Qing Ge
    SomaGenics, Inc, Santa Cruz, California 95060, USA
    RNA 16:118-30. 2010
    ..In this study, the effects of chemical modifications on the potency, serum stability, and innate immune response of sshRNAs were investigated...
  42. pmc Factor H-binding protein is important for meningococcal survival in human whole blood and serum and in the presence of the antimicrobial peptide LL-37
    K L Seib
    Molecular Genetics Unit, Novartis Vaccines, Via Fiorentina, 1, 53100 Siena, Italy
    Infect Immun 77:292-9. 2009
    ..All fHBP null mutant strains analyzed were sensitive to killing in ex vivo human whole blood and serum models of meningococcal bacteremia with respect to the isogenic wild-type strains...
  43. doi Differential effect of platelet-rich plasma and fetal calf serum on bone marrow-derived human mesenchymal stromal cells expanded in vitro
    Anja Goedecke
    University Hospital Carl Gustav Carus, Department of Haematology, Dresden, Germany
    J Tissue Eng Regen Med 5:648-54. 2011
    ..Human platelet-rich plasma has been introduced as an alternative serum source but functional differences have so far not been described...
  44. ncbi Cytokine levels in bronchoalveolar lavage and serum in 3 patients with 2009 Influenza A(H1N1)v severe pneumonia
    Angel Estella
    Intensive Care Unit, Hospital de Jerez, Spain
    J Infect Dev Ctries 5:540-3. 2011
    ..In this study, we assess the inflammatory response mediated by cytokines at the local and systemic levels in three cases of severe pneumonia caused by Influenza A (H1N1) virus...
  45. doi Association of increased serum IL-33 levels with clinical and laboratory characteristics of systemic lupus erythematosus in Chinese population
    Zaixing Yang
    Department of Laboratory Diagnostics, Changzheng Hospital, Second Military Medical University, Shanghai, China
    Clin Exp Med 11:75-80. 2011
    ..The aim of this study was to examine whether the serum IL-33 level is associated with SLE. A total of 70 patients with SLE were recruited...
  46. doi Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess
    Pei Fang Hsieh
    Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan
    J Infect Dis 197:1717-27. 2008
    ..Klebsiella pneumoniae has become the predominant pathogen causing primary pyogenic liver abscess (PLA)...
  47. doi Free thyroid hormones in serum by direct equilibrium dialysis and online solid-phase extraction--liquid chromatography/tandem mass spectrometry
    Bingfang Yue
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108 1221, USA
    Clin Chem 54:642-51. 2008
    ..We describe a direct equilibrium dialysis (ED)-liquid chromatography (LC)/tandem mass spectrometry (MS/MS) method for FT4 and FT3...
  48. pmc Microfluidic isolation and transcriptome analysis of serum microvesicles
    Chihchen Chen
    BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Lab Chip 10:505-11. 2010
    ..present an easy and rapid microfluidic immunoaffinity method to isolate microvesicles from small volumes of both serum from blood samples and conditioned medium from cells in culture...
  49. ncbi Determination of folate vitamers in human serum by stable-isotope-dilution tandem mass spectrometry and comparison with radioassay and microbiologic assay
    Christine M Pfeiffer
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
    Clin Chem 50:423-32. 2004
    ..tandem mass spectrometric method coupled to liquid chromatography (LC/MS/MS) as a candidate reference method for 5-methyltetrahydrofolic acid (5MeTHF), 5-formyltetrahydrofolic acid (5FoTHF), and folic acid (FA) in human serum.
  50. pmc Ultradian oscillations of Stat, Smad, and Hes1 expression in response to serum
    Shigeki Yoshiura
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 104:11292-7. 2007
    b>Serum response has been used as a model for studying signaling transduction for many biological events such as cell proliferation and survival...
  51. ncbi High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell culture
    Chia Chyi Liu
    Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
    Vaccine 25:19-24. 2007
    ..We have previously reported that large-scale preparation of a low immunogenic EV71 strain can be achieved using serum free microcarrier Vero cell culture in a 2-l bioreactor [Wu SC, Liu CC, Lian WC...
  52. pmc miRNAs as serum biomarkers for Duchenne muscular dystrophy
    Davide Cacchiarelli
    Department of Biology and Biotechnology, Institute Pasteur Cenci Bolognetti and IBPM, Sapienza University of Rome, Italy
    EMBO Mol Med 3:258-65. 2011
    ..Despite many different DMD therapeutic approaches are now entering clinical trials, a unifying method for assessing the benefit of different treatments is still lacking...
  53. doi Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry
    Warwick B Dunn
    Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK
    Nat Protoc 6:1060-83. 2011
    ..Protocols for serum- and plasma-based metabolic profiling applying gas chromatography-MS (GC-MS) and ultraperformance liquid ..
  54. doi Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum
    Eva Zelena
    Bioanalytical Sciences Group, School of Chemistry, Manchester Interdisciplinary Biocentre, University of Manchester, M1 7DN, UK
    Anal Chem 81:1357-64. 2009
    A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance Liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS)...
  55. pmc Phosphoethanolamine substitution of lipid A and resistance of Neisseria gonorrhoeae to cationic antimicrobial peptides and complement-mediated killing by normal human serum
    Lisa A Lewis
    Department of Medicine, University of Massachusetts School of Medicine, Worcester, Massachusetts, USA
    Infect Immun 77:1112-20. 2009
    ..cause disseminated gonococcal infection requires that such strains resist the bactericidal action of normal human serum. The bactericidal action of normal human serum against N...
  56. pmc A blood-based screening tool for Alzheimer's disease that spans serum and plasma: findings from TARC and ADNI
    Sid E O'Bryant
    Department of Neurology, F Marie Hall Institute for Rural and Community Health, Texas Tech University Health Sciences Center, Lubbock, Texas, United States of America
    PLoS ONE 6:e28092. 2011
    ..There is no rapid and cost effective tool that can be implemented as a front-line screening tool for Alzheimer's disease (AD) at the population level...
  57. pmc Polymorphism of rs1044925 in the acyl-CoA:cholesterol acyltransferase-1 gene and serum lipid levels in the Guangxi Bai Ku Yao and Han populations
    Dong Feng Wu
    Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China
    Lipids Health Dis 9:139. 2010
    The association of rs1044925 polymorphism in the acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) gene and serum lipid profiles is not well known in different ethnic groups. Bai Ku Yao is a special subgroup of the Yao minority in China...
  58. doi Development of a high-throughput assay for measuring serum neutralizing antibody against enterovirus 71
    Mei Liang Huang
    Vaccine Research and Development Center, National Health Research Institutes, Taiwan
    J Virol Methods 165:42-5. 2010
    ..The serum neutralizing antibody response is the major indicator of EV71 infection and protective immunity...
  59. doi High-abundance proteins depletion for serum proteomic analysis: concomitant removal of non-targeted proteins
    Elisa Bellei
    Department of Laboratory Medicine, Medical Faculty, University Hospital of Modena and Reggio Emilia, Via del Pozzo 71, 41100, Modena, Italy
    Amino Acids 40:145-56. 2011
    In clinical and pharmaceutical proteomics, serum and plasma are frequently used for detection of early diagnostic biomarkers for therapeutic targets...
  60. pmc Strong correlation between liver and serum levels of hepatitis C virus core antigen and RNA in chronically infected patients
    V Descamps
    Virology Department, Amiens University Hospital, South Hospital, Amiens, France
    J Clin Microbiol 50:465-8. 2012
    ..The data showed a strong correlation between liver and serum compartments of HCV Ag levels (r = 0.80) and HCV RNA levels (r = 0.87)...
  61. pmc Diagnostic accuracy of serum 1,3-β-D-glucan for pneumocystis jiroveci pneumonia, invasive candidiasis, and invasive aspergillosis: systematic review and meta-analysis
    Akira Onishi
    Department of Evidence Based Laboratory Medicine and Clinical Pathology and Immunology, Kobe, Japan
    J Clin Microbiol 50:7-15. 2012
    b>Serum 1,3-β-d-glucan (BG) assay may be helpful as a marker for the diagnosis of Pneumocystis jiroveci pneumonia (PJP) and invasive fungal infection (IFI). We conducted a systematic review to assess the diagnostic accuracy of this assay...
  62. pmc Serum from patients with SLE instructs monocytes to promote IgG and IgA plasmablast differentiation
    Hyemee Joo
    Baylor Institute for Immunology Research, Dallas, TX 75204, USA
    J Exp Med 209:1335-48. 2012
    The development of autoantibodies is a hallmark of systemic lupus erythematosus (SLE). SLE serum can induce monocyte differentiation into dendritic cells (DCs) in a type I IFN-dependent manner...
  63. doi Identification of transaldolase as a novel serum biomarker for hepatocellular carcinoma metastasis using xenografted mouse model and clinic samples
    Cun Wang
    Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China
    Cancer Lett 313:154-66. 2011
    ..The goal of this study was to screen and identify potential serum proteins indicating HCC metastasis...
  64. pmc Rho-actin signaling to the MRTF coactivators dominates the immediate transcriptional response to serum in fibroblasts
    Cyril Esnault
    Transcription Group
    Genes Dev 28:943-58. 2014
    The transcription factor SRF (serum response factor) recruits two families of coactivators, the MRTFs (myocardin-related transcription factors) and the TCFs (ternary complex factors), to couple gene transcription to growth factor ..
  65. pmc Human serum promotes Candida albicans biofilm growth and virulence gene expression on silicone biomaterial
    Yuthika Hemamala Samaranayake
    Oral Bio Sciences, Faculty of Dentistry, University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 8:e62902. 2013
    ..We therefore evaluated the effect of human serum on C. albicans biofilm morphology, growth, and the expression of virulence-related genes on SB in vitro.
  66. doi Relationships between disease activity and serum and fecal biomarkers in patients with Crohn's disease
    Jennifer Jones
    Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Clin Gastroenterol Hepatol 6:1218-24. 2008
    ..between the Crohn's Disease Activity Index (CDAI), the Simple Endoscopic Score for Crohn's Disease (SES-CD), serum high-sensitivity C-reactive protein (hsCRP) (both phenotype and genotype) and interleukin-6 (IL-6), and fecal ..
  67. pmc MALDI-TOF mass spectral analysis of siRNA degradation in serum confirms an RNAse A-like activity
    John J Turner
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, UKCB2 2QH
    Mol Biosyst 3:43-50. 2007
    ..b>Serum stability is a major concern if siRNA is to be used for therapeutic delivery within blood circulation...
  68. pmc Early serum galactomannan trend as a predictor of outcome of invasive aspergillosis
    Louis Y A Chai
    Department of Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Clin Microbiol 50:2330-6. 2012
    ..We determined whether serum galactomannan index (GMI) trends early in the course of disease may be useful in predicting eventual clinical ..
  69. pmc Serum levels of alanine aminotransferase decrease with age in longitudinal analysis
    Mamie H Dong
    Division of Gastroenterology, Department of Medicine, University of California San Diego, La Jolla, California 92093, USA
    Clin Gastroenterol Hepatol 10:285-90.e1. 2012
    ..The mean ALT level has been reported to decrease with age; we performed a longitudinal analysis to determine whether serum levels of ALT changes with age among community-dwelling, older adults in the US.
  70. pmc Characterization of the acute and persistent pain state present in K/BxN serum transfer arthritis
    Christina A Christianson
    Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA
    Pain 151:394-403. 2010
    ..We characterized the K/BxN serum transfer arthritis model as a model of joint inflammation-induced pain and examined pharmacologic responsiveness ..
  71. doi Plasma versus serum levels of osteopontin and mesothelin in patients with malignant mesothelioma--which is best?
    Jenette Creaney
    National Research Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, 4th Floor, G Block, Verdun St, Nedlands, WA 6009, Australia
    Lung Cancer 74:55-60. 2011
    ..As there is some concern about the sensitivity of osteopontin in serum versus plasma, we compared them in the same patient population to mesothelin.
  72. doi Effects of SMYD3 overexpression on transformation, serum dependence, and apoptosis sensitivity in NIH3T3 cells
    Xue Gang Luo
    Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China
    IUBMB Life 61:679-84. 2009
    ..g., STAT1, MAP3K11, and PIK3CB). To determine the effects of SMYD3 overexpression on cell transformation, serum dependence and apoptosis sensitivity, we expressed SMYD3 in NIH3T3 cells, and these cells showed several ..
  73. doi Replicative aging and differentiation potential of human adipose tissue-derived mesenchymal stromal cells expanded in pooled human or fetal bovine serum
    Karen Bieback
    Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service Baden Württemberg Hessen, Germany
    Cytotherapy 14:570-83. 2012
    ..manufacturing, different supplements have been evaluated as alternatives for the commonly used fetal bovine serum (FBS)...
  74. ncbi Gradient generation by an osmotic pump and the behavior of human mesenchymal stem cells under the fetal bovine serum concentration gradient
    Joong Yull Park
    Department of Biomedical Engineering, College of Medicine, Korea University, Anam Dong, Seongbuk gu, Seoul 136 705, Republic of Korea
    Lab Chip 7:1673-80. 2007
    ..pump was evaluated by culturing human mesenchymal stem cells within the concentration gradient of fetal bovine serum. The effects of the gradient on attachment, viability and morphology of the cells were analyzed and quantified...
  75. pmc Mesenchymal stem cells are injured by complement after their contact with serum
    Yan Li
    Department of Pathology, Case Western Reserve University, Cleveland, OH, USA
    Blood 120:3436-43. 2012
    ..this report, we demonstrated that human MSCs activated complement of the innate immunity after their contact with serum. Although all 3 known intrinsic cell-surface complement regulators were present on MSCs, activated complement ..
  76. pmc Effects of smoking and smoking cessation on human serum metabolite profile: results from the KORA cohort study
    Tao Xu
    Research Unit of Molecular Epidemiology, Helmholtz Zentrum Munchen, Ingolstädter Landstraße 1, Neuherberg 85764, Germany
    BMC Med 11:60. 2013
    ..Here, we extend our metabolomics study with a large prospective study including female smokers and quitters...
  77. doi Serum insulin-like growth factor-1 levels predict outcomes of patients with advanced hepatocellular carcinoma receiving antiangiogenic therapy
    Yu Yun Shao
    Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan
    Clin Cancer Res 18:3992-7. 2012
    Patients with liver cirrhosis or hepatocellular carcinoma (HCC) have decreased serum insulin-like growth factor (IGF)-1 levels...
  78. pmc Bordetella pertussis autotransporter Vag8 binds human C1 esterase inhibitor and confers serum resistance
    Nico Marr
    Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
    PLoS ONE 6:e20585. 2011
    ..pertussis factor was not identified. Here we set out to identify the B. pertussis C1inh-binding factor. Using a serum overlay assay, we found that this factor migrates at approximately 100 kDa on an SDS-PAGE gel...
  79. pmc First time molecular detection and phylogenetic relationships of torque teno sus virus 1 and 2 in domestic pigs in Uganda: further evidence for a global distribution
    Matilda Brink
    Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Virol J 9:39. 2012
    ..Therefore, the aim of this study was to investigate the possible presence of the two viruses in domestic pigs in Uganda, and describe the phylogenetic relationships to those in the rest of the world...
  80. doi Enrichment of peptides in serum by C(8)-functionalized magnetic nanoparticles for direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis
    Ning Yao
    Department of Chemistry, Fudan University, Shanghai 200433, China
    J Chromatogr A 1185:93-101. 2008
    Human serum contains a complex array of proteolytically derived peptides (serum peptidome), which contain biomarkers of preclinical screening and disease diagnosis...
  81. ncbi Increased oxidative DNA damage, as assessed by urinary 8-hydroxy-2'-deoxyguanosine concentrations, and serum redox status in persons exposed to mercury
    Chunying Chen
    Key Laboratory of Nuclear Analytical Techniques and Laboratory for Nanoscale Materials and Related Bio Environmental Sciences, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, Peoples Republic of China
    Clin Chem 51:759-67. 2005
    ..In this study, we evaluated the relationship between mercury exposure and oxidative stress, serum and urinary mercury concentrations, oxidative DNA damage, and serum redox status in chronically mercury-exposed ..
  82. ncbi Serum BDNF levels and weight gain in schizophrenic patients on long-term treatment with antipsychotics
    Xiang Yang Zhang
    Menninger Department of Psychiatry and Behavioral Sciences, Baylor College of Medicine, VA Medical Center, Houston, TX 77030, USA
    J Psychiatr Res 41:997-1004. 2007
    ..Reports have also shown that serum BDNF is altered in schizophrenic patients treated with antipsychotics...
  83. doi A multi-metabolite analysis of serum by 1H NMR spectroscopy: early systemic signs of Alzheimer's disease
    Taru Tukiainen
    Computational Medicine Research Group, Department of Biomedical Engineering and Computational Science, Helsinki University of Technology, P O Box 9203, FI 02015 HUT, Finland
    Biochem Biophys Res Commun 375:356-61. 2008
    A three-molecular-window approach for (1)H NMR spectroscopy of serum is presented to obtain specific molecular data on lipoproteins, various low-molecular-weight metabolites, and individual lipid molecules together with their degree of (..
  84. pmc Differences between Trypanosoma brucei gambiense groups 1 and 2 in their resistance to killing by trypanolytic factor 1
    Paul Capewell
    College of Medical, Veterinary and Biological Sciences, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom
    PLoS Negl Trop Dis 5:e1287. 2011
    ..b. brucei is unable to infect humans due to sensitivity to trypanosome lytic factors (TLF) 1 and 2 found in human serum. T. b. rhodesiense and T. b. gambiense are able to resist lysis by TLF. There are two distinct sub-groups of T. b...
  85. doi Serum starvation induces H2AX phosphorylation to regulate apoptosis via p38 MAPK pathway
    Chengrong Lu
    The Aviation Medicine Research Lab, General Hospital of Air Force, Fucheng Road 30, Beijing 100036, China
    FEBS Lett 582:2703-8. 2008
    ..Here we show that serum starvation induces H2AX phosphorylation and apoptosis independent of the JNK pathway...
  86. doi Serum concentrations of ribavirin and pegylated interferon and viral responses in patients infected with HIV and HCV
    Florence Nicot
    INSERM, U 563, Toulouse, France
    J Med Virol 80:1523-9. 2008
    ..This study investigated the relationship between the serum pegylated interferon and ribavirin concentrations 3 and 6 months after treatment initiation, and treatment outcome ..
  87. ncbi Inhibition of vascular smooth muscle cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation
    Shijun Wang
    Department of Cardiovascular Disease, The First Affiliated Hospital, Medical School of Zhejiang University, Hangzhou 310003, China
    J Ethnopharmacol 114:458-62. 2007
    This study was to investigate the effect of serum from rats treated orally with GUD on vascular smooth muscle cells (VSMCs) proliferation in vitro.
  88. doi Monoclonal antibodies raised against the ORF3 protein of hepatitis E virus (HEV) can capture HEV particles in culture supernatant and serum but not those in feces
    Masaharu Takahashi
    Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311 1 Yakushiji, Shimotsuke shi, Tochigi Ken 329 0498, Japan
    Arch Virol 153:1703-13. 2008
    ..The anti-ORF3 MAbs could capture HEV particles in culture medium and serum at variable efficiency of up to 61 and 49%, respectively, but not those in feces...
  89. ncbi Characterization of a uricase from Bacillus fastidious A.T.C.C. 26904 and its application to serum uric acid assay by a patented kinetic uricase method
    Yunsheng Zhao
    Laboratory of Bioinformatics and Molecular Engineering, Chongqing University of Medical Sciences, Chongqing 400016, People s Republic of China
    Biotechnol Appl Biochem 45:75-80. 2006
    An intracellular uricase from Bacillus fastidious A.T.C.C. 26904 was characterized and evaluated for serum uric acid assay by a patented kinetic uricase method. The active uricase was 151 kDa by gel filtration through Sephadex G-200...
  90. doi A population of serum deprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells
    Steven Sauerzweig
    Leibniz Institute for Neurobiology IfN, Projectgroup Neuropharmacology, Brenneckestr 6, 39118 Magdeburg, Germany
    Exp Cell Res 315:50-66. 2009
    ..The complete removal of serum might offer a simple way to specifically enrich this fraction of pluripotent embryonic like stem cells in BMSC ..
  91. doi The effects of serum on the toxicity of manufactured nanoparticles
    Martin J D Clift
    Centre for Nano Safety, School of Life Sciences, Edinburgh Napier University, Merchiston Campus, 10 Colinton Road, Edinburgh EH10 5DT, United Kingdom
    Toxicol Lett 198:358-65. 2010
    The aim of the present study was to assess the effects of the presence and absence of serum in NP suspension media in relation to their cytotoxicity, as well as their potential to cause oxidative stress and stimulate pro-inflammatory ..
  92. ncbi Growth stimulation leads to cellular senescence when the cell cycle is blocked
    Zoya N Demidenko
    Oncotarget, Albany, New York, USA
    Cell Cycle 7:3355-61. 2008
    ..In the presence of serum, induction of p21 caused senescence, characterized by beta-Galactosidase staining, cell hypertrophy, increased ..
  93. doi The CO-releasing molecule CORM-3 protects against articular degradation in the K/BxN serum transfer arthritis model
    Nuria Maicas
    Department of Pharmacology, University of Valencia, Av Vicent Andres Estelles s n, 46100 Burjasot, Valencia, Spain
    Eur J Pharmacol 634:184-91. 2010
    ..Arthritis was induced in C57Black-6 mice by an intraperitoneal injection of serum from arthritic K/BxN mice...
  94. pmc Serum antibody response to five Streptococcus pneumoniae proteins during acute otitis media in otitis-prone and non-otitis-prone children
    Ravinder Kaur
    Rochester General Hospital Research Institute, Center for Infectious Diseases and Immunology, Rochester, NY 14621, USA
    Pediatr Infect Dis J 30:645-50. 2011
    ..Streptococcus pneumoniae (Spn) is one of the common bacteria responsible for episodic acute otitis media (AOM; non-otitis-prone), recurrent AOM (otitis-prone), and AOM treatment failure (AOMTF) in children...
  95. ncbi Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use
    Norbert Stute
    Bone Marrow Transplant Center, University Hospital Hamburg Eppendorf, Hamburg, Germany
    Exp Hematol 32:1212-25. 2004
    ..One major obstacle for their clinical use is the biosafety of fetal calf serum (FCS), which is a crucial part of all media currently used for the culture of MSC.
  96. ncbi Comparison of human serum with fetal bovine serum for expansion and differentiation of human synovial MSC: potential feasibility for clinical applications
    K Tateishi
    Department of Orthopedics, National Hospital Organization Kure Medical Center, Hiroshima, Japan
    Cell Transplant 17:549-57. 2008
    The aim of this study was to evaluate the effect of human serum (HS) on growth and differentiation capacity of human synovium-derived mesenchymal stem cells (MSC) in comparison to cells grown in fetal bovine serum (FBS)...
  97. ncbi Higher amount of free circulating DNA in serum than in plasma is not mainly caused by contaminated extraneous DNA during separation
    Naoyuki Umetani
    Department of Molecular Oncology, John Wayne Cancer Institute, 2200 Santa Monica Blvd, Santa Monica, CA 90404, USA
    Ann N Y Acad Sci 1075:299-307. 2006
    Circulating DNA isolated from serum and plasma has been shown to be a useful biomarker in various diseases including cancer. Serum reportedly contains a higher amount of free circulating DNA than it does in plasma...
  98. pmc Activation and repression of cellular immediate early genes by serum response factor cofactors
    Seung Min Lee
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 285:22036-49. 2010
    The induction of expression of many cellular immediate early genes (IEG) involves the transcription factor serum response factor (SRF)...
  99. pmc Detection of dengue virus in saliva and urine by real time RT-PCR
    Telma R Poloni
    Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de Ciencias Farmaceuticas, Universidade de Sao Paulo, Ribeirão Preto14040 903, Sao Paulo, Brasil
    Virol J 7:22. 2010
    ..b>Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and ..
  100. pmc Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors
    Reza Ardehali
    Department of Internal Medicine, Division of Cardiovascular Medicine, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 108:3282-7. 2011
    ..that overexpression of the prosurvival gene BCL2 in mouse embryonic stem cells overrode the requirement of serum factors and feeder cells to maintain mESCs in culture...
  101. doi A well-characterised peak identification list of MALDI MS profile peaks for human blood serum
    Ali Tiss
    The BioCentre and Department of Chemistry, University of Reading, Reading, UK
    Proteomics 10:3388-92. 2010
    MALDI MS profiling, using easily available body fluids such as blood serum, has attracted considerable interest for its potential in clinical applications...

Research Grants82

  1. Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays
    Ryan Bailey; Fiscal Year: 2009
    ..This technology promises to broadly impact the landscape of the biomedical sciences, both meeting the clinical diagnostic challenges of today and pioneering the paradigm-shifting discoveries of tomorrow. ..
  2. Xenoestrogen regulation of Leydig cells
    ..The P.I. hypothesizes that disparities in serum androgen levels and Leydig cell steroidogenic capacity are linked to differences in the numbers of Leydig cells...
    Charles Knott; Fiscal Year: 2010
    ..Contrary to earlier investigations, however, the risk of breast cancer was not associated with serum levels of DDT among women in Mexico City...
    Charles Lynch; Fiscal Year: 2010
    ..Contrary to earlier investigations, however, the risk of breast cancer was not associated with serum levels of DDT among women in Mexico City...
    Thomas A Platts Mills; Fiscal Year: 2010
    ..markers such as eosinophilia, exhaled NO (eNO) and sinus CT score;as well as measurements of cytokines in serum and leukotrienes in urine...
  6. The Primate Corpus Luteum: Functional Regression and Cardiovascular Impacts
    Randy L Bogan; Fiscal Year: 2012
    ..b>Serum levels of estradiol (E2) and P4, as well as the first day of menstruation, will be used as markers of luteal ..
  7. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..We have documented that serum or plasma from FSGS patients increases glomerular albumin permeability (Palb) and that the specific activity of ..
  8. Cardiovascular Responses to Sleep Apnea in Tetraplegia: A Pilot Study
    Jill M Wecht; Fiscal Year: 2010
    ..A secondary objective of this pilot investigation will be to investigate whether nocturnal plasma renin and serum aldosterone concentrations differ among individuals with tetraplegia and OSA compared to their counterparts ..
  9. Iron-mediated vascular disease in sickle cell disease.
    John C Wood; Fiscal Year: 2010
    ..Pancreatic R2* will be measured as a surrogate for chronic labile iron exposure. Serum ferritin, transferrin saturation, and labile plasma iron (LPI) will be measured as acute markers of iron status...
  10. Pilot and definitive studies to evaluate Insertional Mutagenesis
    LU TAYLOR; Fiscal Year: 2012
    ..glutathione, clinical pathology, hepatic RNA isolation and subsequent microarray profiling, liver proteomics and serum multiple analyte profiles (MAPs) will be determined in this study...
  11. Studies to Evaluate the Toxicological Potential of Test Articles
    LU TAYLOR; Fiscal Year: 2010
    ..glutathione, clinical pathology, hepatic RNA isolation and subsequent microarray profiling, liver proteomics and serum multiple analyte profiles (MAPs) will be determined in this study...
  12. Importance of antigen specific immunoglobulin responses in controlling SIV/SHIV i
    Bapi Pahar; Fiscal Year: 2010
    ..We will also compare quantitative and qualitative binding properties of all longitudinal plasma/serum samples and/or mucosal secretions from macaques with different levels of immunity to trimeric recombinant SIV/..
  13. Comprehensive biomarker study to capitalize on existing GWAS in 10K South Asians
    Danish Saleheen; Fiscal Year: 2010
    ..D, parathyroid hormone);and (4) inflammation and hemostasis (hsCRP, MCP-1, IL-6, sTNFRII, sVCAM-1, sICAM-1, serum amyloid A, MMP-9, E-selectin, P-selectin, CD40 ligand, fibrinogen, von Willebrand factor, and D-dimer)...
  14. Peroxynitrite Decomposition Catalyst and Nitric Oxide Donor for Endotoxemia
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..b>Serum and tissue will be examined 48 h post LPS dosing in each of the above Aims for determination of morphologic and ..
  15. Prostate, Lung, Colorectal and Ovarian (PLCO) Screening Trial
    BARBARA O'BRIEN; Fiscal Year: 2013
    ..Whole blood, sequential serum samples and one plasma sample were collected from the intervention arm...
  16. Prostate, Lung, Colorectal and Ovarian (PLCO) Screening Trial
    Mona Fouad; Fiscal Year: 2013
    ..Whole blood, sequential serum samples and one plasma sample were collected from the intervention arm...
  17. Vitamin D3, Interference with Androgen Signal and Inhibition of Prostate Cancer
    Bandana Chatterjee; Fiscal Year: 2013
    ..Vitamin D deficiency is a common problem among the elderly. Population based research has shown that low serum vitamin D levels in men correlate with increased prostate cancer risk...
  18. Effects of Evoked Resistance Training and Testosterone after Spinal Cord Injury
    Ashraf Gorgey; Fiscal Year: 2012
    ..the changes in metabolic milieu (resting energy expenditure, glucose homeostasis, lipid profile, free fatty acids, serum total and free testosterone and IGF-1), and cytokines (c-reactive protein, tumor necrosis factor alpha and IL-6 as ..
  19. Retinoid and Carotenoid Depletion in Patients at High-Risk for Liver Cancer
    Peter H Gann; Fiscal Year: 2010
    ..of oxidative stress that can serve as surrogate endpoints in early trials, and c) determine how biomarkers in serum and urine relate to antioxidant levels in the liver itself and to tissue markers of pre-neoplastic change...
  20. Reactive (AA)Amyloidosis
    Merrill D Benson; Fiscal Year: 2013
    ..or genetic, cause recurrent inflammation resulting in elevated expression of the acute phase proteins of the serum amyloid A (SAA) family. SAA, an apolipoprotein of plasma HDL, is the precursor of the amyloid fibril subunit AA...
  21. Pituitary dysfunction, behavioral symptoms, and quality of life after blast mTBI
    ..changes in body composition, and poorer QoL;and 4) to determine whether a combination of basal hormone measures, serum lipid profiles, body composition indices, and additional demographic, behavioral, and symptom values can be used ..
  22. Role of SCD1 and Dietary Methionine Restriction on Energy Homeostasis
    ERIC PAUL PLAISANCE; Fiscal Year: 2011 mice results in an increase in food intake accompanied by elevations in energy expenditure and reductions in serum triglycerides and adiposity...
  23. Cellular &Inflammatory Pathways in Ashtma &Allergic Diseases: From IgE to Cells
    LAWRENCE BARRY SCHWARTZ; Fiscal Year: 2012
    ..containing eosinophilic leukocytes, lymphocytes, and mast cells, increased mucus secretion, and elevated serum IgE levels. Type 2 helper T lymphocytes (Th2) play a critical role in orchestrating allergic airway inflammation...
    Melanie H Cobb; Fiscal Year: 2012
    ..We will also elucidate mechanisms of regulation of the epithelial sodium channel (ENaC) by WNK1. The serum- and glucocorticoid-inducible protein kinase SGK1 phosphorylates the E3 ubiquitin ligase neural precursor cell ..
    Michael J LaMonte; Fiscal Year: 2013
    ..examiners, the well-defined sample of postmenopausal women, DXA measures of body composition, and measured serum biomarkers...
  26. Iron, Metabolic Regulation and Type 2 Diabetes Risk
    Donald A McClain; Fiscal Year: 2013
    ..conditions of iron overload such as hereditary hemochromatosis (HH) but even for dietary iron excess resulting in serum ferritin concentrations within the higher ranges of "normal...
  27. Mechanisms of hepcidin effects in the anemia of chronic disease
    Robert T Means; Fiscal Year: 2013
    ..This disorder typically manifests itself as a hypoproliferative anemia accompanied by a low serum iron concentration despite adequate reticuloendothelial iron stores...
  28. DNA methylation of extracellular superoxide dismutase in pulmonary hypertension
    EVA NOZIK GRAYCK; Fiscal Year: 2012
    ..Aim 1 will utilize lung and pulmonary artery tissue and serum procured at the time of organ transplantation in patients with IPAH, disease-specific controls with PAH due to ..
  29. Retinoid Metabolism and Alcohol Induced Disease
    William S Blaner; Fiscal Year: 2010
    ..Hepatic retinoid storage and mobilization are normal in healthy MCK-LpL0 mice since serum and hepatic retinoid levels are not different for these mice compared to matched chow fed wild type (WT) mice...
  30. Hepcidin-based screening for infantile iron deficiency
    Mark D Fleming; Fiscal Year: 2010
    ..This proposal will evaluate a new test, the serum hepcidin, as a screen for ID in infants...
  31. Molecular Markers for Progression of Pulmonary Fibrosis in Systemic Sclerosis
    Shervin Assassi; Fiscal Year: 2013
    ..The objective of the current proposal is to build multivariate models with gene expression profiling and serum chemokine data that will have greater predictive value for the course of ILD over the currently known parameters...
  32. Alpha B crystallin as a novel therapeutic for SIRS and Sepsis
    Jonathan B Rothbard; Fiscal Year: 2011
    ..reduce in a dose dependent manner serological levels of IL-6, serum amyloid A, and C reactive protein in mice injected with LPS and (ii...
  33. Pathobiology of Cystic Fibrosis-Related Diabetes in a Ferret Model
    Zoe Stewart; Fiscal Year: 2013
    ..We will assess the effectiveness of pancreatic CFTR gene replacement in the CFTR-/- ferrets by evaluating serum glucose, insulin, glucagon, endocrine pancreatic function, and pancreatic duct cell physiology...
  34. Chronic Sleep Restriction Increases Immunity to Autoantigen: Role of the SNS
    Arnold E Postlethwaite; Fiscal Year: 2010
    ..the immune response to CII is enhanced with increased production of interferon3 and other Th1 cytokines, increased serum levels of anti-CII antibodies, decreased CD8+ T cells, increased CD11b+ cells and changes in plasma levels of ..
  35. Ethanol Regulation of Serum Glucocorticoid Kinase I
    BLAIR N COSTIN; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Previous microarray studies in the Miles'laboratory have shown that serum and glucocorticoid- regulated kinase 1, Sgk1, is prominently up regulated by acute ethanol (2 g/kg) in the prefrontal ..
  36. Steroid Responsive Mechanisms in the Ear
    DENNIS ROYAL TRUNE; Fiscal Year: 2012
    ..morphology will be assessed by light and electron microscopy;3) systemic autoimmune disease will be assessed by serum immune complexes, hematocrits, and antinuclear antibodies;4) cochlearspecificautoantibodies ..
    Lisa M Salati; Fiscal Year: 2012
    ..In addition, the amount and type of fat in the diet can regulate the levels of serum cholesterol and triglycerides, risk factors for atherosclerosis...
  38. Nicotinic Acetylcholine Receptor Density and Veteran Cigarette Smokers
    Arthur Brody; Fiscal Year: 2013
    ..demonstrate that menthol itself inhibits nicotine metabolism, and that menthol cigarette smoking leads to elevated serum levels of nicotine and cotinine, and greater exhaled carbon monoxide levels...
  39. New insights in mechanisms of renal injury
    Balakuntalam S Kasinath; Fiscal Year: 2013
    ..A. Upstream regulators of GSK 3beta. We will explore the role of serum and glucocorticoid-regulated kinase1 (SGK1), p70S6 kinase1 (p70S6k1), and p90rsk as upstream regulators of GSK ..
  40. Ubiquitous pollutant acrolein inhibits IFN?? antiviral signaling: relevance to HC
    Swati Joshi-Barve; Fiscal Year: 2013
    ..establish a clinical/translational aspect of our hypothesis by conducting a retrospective analysis using banked serum, urine and/or liver biopsy samples from healthy, uninfected individuals and HCV-infected patients (responders and ..
  41. Hepatic Steatosis Modulation by Apo B Gene Transcription
    JANET DEHOFF SPARKS; Fiscal Year: 2010
    ..Understanding the mechanism responsible for the action of betaine on apo B and serum lipoproteins could improve therapy by specific intervention by enhancing TG export from the liver...
  42. Targeting iNOS to inhibit myeloid-derived suppressor cells (MDSC) in melanoma
    ANDREW GREGORY SIKORA; Fiscal Year: 2013
    ..b>Serum VEGF levels were also suppressed, intratumoral MDSC infiltration reduced, and T cell numbers normalized in tumor-..
  43. Immune Mechanism of Rejection in Human Lung Allografts
    Thalachallour Mohanakumar; Fiscal Year: 2013 ISHLT criterion), 3) reducing the levels of pro-inflammatory cytokines (IFN-?, IL-6 and IL-17) in the serum (determined by Luminex), 4) decreasing the frequency of cells secreting pro-inflammatory cytokines (IFN-? and IL-..
  44. Bisphosphonates in the treatment of chronic kidney disease-mineral bone disease
    Sharon M Moe; Fiscal Year: 2013 treating CKD and normal rats with zoledronic acid at early and late time points and determine the effect of serum measures of mineral homeostasis, bone turnover, architecture and biomechanics, and arterial calcification, aorta ..
  45. Osteocalcin and Metabolic Risk Factors. The Framingham Study
    Yi Hsiang Hsu; Fiscal Year: 2011
    ..Thus the underlying hypothesis of this proposal is that there are shared genetic variants that regulate both serum OC and metabolic risk factors (metabolic syndrome risk factor clustering: a combination of risk factors including ..
  46. Analyzer for Monitoring Personal Environmental Exposure to Fluids and Materials
    MATTHEW CUSACK; Fiscal Year: 2010
    ..The presence of metals is well-known in most neurodegenerative pathologies, such as excess serum copper or brain iron accumulation, which are associated with Wilson's, Alzheimer's, and Parkinson's ..
  47. A Thioredoxin Mimetic for Radiocontrast Nephropathy
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..peroxidation, neutrophil infiltration, apoptosis, necrosis, peroxynitrite and poly(ADP- ribose) formation, and serum concentrations of neutrophil gelatinase-associated lipocalin...
  48. Global regulatory interactions in bacterial pathogenesis
    BRIAN AKERLEY; Fiscal Year: 2009
    ..ArcA regulates several virulence phenotypes including LOS outer core modification, serum resistance, and resistance to oxidative stress...
  49. Novel diagnostic and safety biomarkers of liver injury and hepatotoxicity
    Stanislav I Svetlov; Fiscal Year: 2010
    ..Enzymatic assays of serum ALT and AST, widely used as part of a `hepatic function'panel, are neither sensitive nor particularity ..
  50. Expression of sFlt1 and its function in the glomerular endothelium
    Christie P Thomas; Fiscal Year: 2013
    ..b>Serum from toxemic women stimulates the glomerular endothelial cell (GEC) to release soluble factors such as endothelin-..
  51. Studying the effects of vitamin D on FOXP3 and IL-17 expression in human CD4+ T c
    Insoo Kang; Fiscal Year: 2010
    ..Of interest, decreased serum levels of 25(OH)VD3 have been reported in patients with autoimmune diseases including rheumatoid arthritis (RA), ..
  52. Regulation of Bile Acid Synthesis by Nuclear Receptors
    John Y L Chiang; Fiscal Year: 2013
    ..Bile acids are known to reduce serum triglyceride levels and play critical role in maintaining glucose and lipid homeostasis, however, the underlying ..
  53. Parathyroid Hormone & Vitamin D Effects on Salt Sensitivity and Vascular Function
    ANNA LIZA BELEN VALINA TOTH; Fiscal Year: 2010
    ..However, maintenance of serum calcium concentrations by chronic PTH elevations has known deleterious physiological effects within the ..
  54. Weight Loss &Exercise Effects on Telomere Length in Postmenopausal Women
    ANNE M MCTIERNAN; Fiscal Year: 2012 the trial: DNA damage repair (COMET), sex hormones, insulin &glucose, inflammation (C-reactive protein, serum amyloid A, interleukin-6), insulin like growth factor 1 and binding protein 3, and adipokines (adiponectin, leptin)..
  55. Thyroid Hormone Action
    THOMAS STERLING SCANLAN; Fiscal Year: 2013
    ..Circulating T1AM is tightly bound to a unique serum binding protein which we have recently identified as apoB-100, the major protein component of low density ..
    Jarmo Virtamo; Fiscal Year: 2010
    ..dietary, smoking, and occupational data were obtained, along with physical measurements, a chest x-ray, and serum and toenail samples...
  57. MR Monitoring of PTC124 Treatment in DMD
    KRISTA H VANDENBORNE; Fiscal Year: 2010
    ..and outcome measures for clinical trials have been limited to measures of muscle function and quality of life, serum biomarkers of muscle breakdown and invasive muscle biopsies...
  58. Nitric Oxide in Hemodialysis AV Fistula Maturation
    Richard A Cohen; Fiscal Year: 2010
    ..for neointimal growth in newly created fistulas, we will evaluate proliferation and migration in response to serum growth factors as well as their inhibition by NO, and compare the responses between cells derived from veins that ..
  59. Regulation of Hepatic SphK2 by Bile Acids: Effects on Lipid Metabolism
    HUIPING ROSE ZHOU; Fiscal Year: 2013
    ..sphingosine kinase 2 (SphK2) expression (at both mRNA and protein levels) and enzyme activity and increase serum sphingosine-1-phosphate (S1P) levels...
  60. Bioassay for Breast Cancer Prevention
    JoEllen Welsh; Fiscal Year: 2010
    ..the diets of mice prior to removal of the glands for culture and thus correlation can be made between dietary/serum levels of nutrients and effectiveness in the bioassay...
  61. Predictors of Pregnancy Outcome in SLE and APS
    Jane E Salmon; Fiscal Year: 2012 7 centers, obtained detailed medical and obstetrical information monthly, and serially collected plasma, serum, DNA, RNA, and urine...
  62. Early Life Conditions, Survival, and Health: A Pedigree-Based Population Study
    Ken R Smith; Fiscal Year: 2013
    ..Biomarker measures studied include C-reactive protein, white blood count, albumin, uric acid, creatinine, serum cholesterol, glycosylated hemoglobin, dehydroepiandrosterone sulfate (DHEAS &DHEA), fasting glucose and ..
  63. Predicting Bilirubin-induced Auditory Toxicity in Infants with Severe Jaundice
    Sanjiv B Amin; Fiscal Year: 2010
    ..AAP) guidelines for treatment to prevent bilirubin-induced neurotoxicity are based on measures of total serum bilirubin, but the utility of this indicator lacks evidence: it has high sensitivity but low specificity for ..
  64. CFI Inactivation of Human Plasma
    TREVOR CASTOR; Fiscal Year: 2009
    ..inactivation) process inactivates enveloped viruses such as MuLV, VSV, TGE, BVD, Sindbis and HIV in fetal bovine serum by a lipid solubilization mechanism, similar to the solvent detergent method...
  65. Biomarker for liver ischemia/reperfusion-induced injury
    MONIKA OLI; Fiscal Year: 2006
    ..Current enzymatic assays of serum ALT or LDH are either non-specific or not sensitive enough to detect early stages of liver damage, assess ..
  66. Point-of Care System for Determination of Bilirubin Capacity in Neonates
    GLEN D RAMSAY; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Human serum albumin is capable of binding bilirubin with high affinity, thus sequestering it and mitigat- ing its harmful neurological effects in jaundiced newborns...
  67. Saliva and Serum Inflammatory Biomarkers in Periodontitis: A Study in Older Women
    Jean Wactawski Wende; Fiscal Year: 2010
    ..The goal of this proposed study is to measure a series of inflammatory biomarkers in saliva and serum to examine to what extent non-invasive salivary biomarkers can be used to characterize those same biomarkers ..
  68. Virus Electrodes for Fungal Pathogen Detection
    Gary Fujii; Fiscal Year: 2009
    ..I SBIR AT application are aimed at demonstrating the ability of the viratrode to rapidly test a sample of blood, serum, urine or bronchoalveolar lavage fluid for the presence of an Aspergillus specific marker...
  69. Proteomic signatures of an early life asthma-protective exposure
    Serrine S Lau; Fiscal Year: 2010
    ..Our specific aim is to identify and characterize proteomic signatures of early life dog exposure in the plasma or serum of children who have or have not been exposed to dogs in early life, and who were or were not diagnosed with ..
  70. Risk factors for encephalopathy in newborns with severe hyperbilirubinemia
    The American Academy of Pediatrics guidelines for intervention in patients with hyperbilirubinemia (total serum bilirubin concentration [TSB] modified by clinical factors) are based on sparse published clinical evidence and do not ..
  71. Long Term Outcomes in ICU Patients: Delirium & Apolipoprotein E
    Sheila Alexander; Fiscal Year: 2009
    ..A secondary goal of the proposed study is to explore the potential relationship between APOE 4 allele presence, serum apolipoprotein E protein levels, serum cytokine levels (IL-1, IL-6, IL-8 and IL-10) and development and duration ..
  72. Neuropsychological & Thyroid Effects of Perfluorinated Compounds in Older Adults
    Edward Fitzgerald; Fiscal Year: 2009
    ..Remaining serum was archived and analyzed in 2005-06 for PBDEs, DLCs, and thyroid hormones...
  73. Serum IGFs--Effects of a Soy Intervention in Humans
    Johanna Lampe; Fiscal Year: 2002
    ..This reduces IGF-I activity, but also increases its half-life in blood. In observational studies, high serum IGF-I, low serum IGFBP-3, or a high molar ratio of IGF-I relative to IGFBP-3 are associated with increased risk of ..
  74. Supplemental Vitamin D and Functional Outcomes in Early Adolescence
    Richard D Lewis; Fiscal Year: 2010
    ..that a significant number of children, particularly those with darker skin pigmentation, have inadequate levels of serum 25-hydroxyvitamin D [25(OH)D]...
  75. Fatty Acid Synthase Inhibitors: Ovarian Cancer Therapy
    ALBERT OWENS; Fiscal Year: 2007
    ..enroll women with recurrent disease that have failed initial therapy and overexpresses FAS, as detected by the serum FAS assay. Serum CA-125 and serum FAS will be added to the usual disease monitors and measures of progression...
    Glenn Gourley; Fiscal Year: 2005
    ..central hypothesis of this proposal is that proteomic techniques can be used to establish a relationship between serum proteins and hepatic fibrosis that will allow determination of hepatic fibrosis without a liver biopsy in patients ..
  77. Anti-GM1b IgM for Detection of Localized Prostate Cancer
    Mepur Ravindranath; Fiscal Year: 2005
    ..Prostate-specific antigen (PSA) is widely used for diagnosing and monitoring CaP. However, serum PSA level does not increase significantly until tumor volume exceeds 1cc, and it is inversely related to a tumor's ..
    David Harpole; Fiscal Year: 2000
    ..Thr purpose of this project is to define tissue and serum tumor markers in patients with stage I NSCLC which predict for early cancer recurrence...
  79. The New England Family Study: Fifty Year Post-Perinatal Follow-Up for Life Course
    ERIC contact LOUCKS; Fiscal Year: 2010
    ..g. intrauterine growth restriction, maternal serum cortisol levels, maternal smoking and socioeconomic adversity) with epigenetic processes (often measured as DNA ..
  80. Gene Delivery of P53 in a Tumor-bearing Mouse Model
    ARCHIBALD MIXSON; Fiscal Year: 2007
    ..polymers that in combination with liposomes significantly increased transfection in the presence or absence of serum. To test the overall hypothesis that further understanding of HK enhancement of transfection will facilitate the ..
  81. Mouse Models to Characterize the Role of Lrp6 in Metabolic Syndrome
    Bart O Williams; Fiscal Year: 2010
    ..developed CVD at a very early age due to metabolic syndrome (a constellation of symptoms which includes high serum levels of LDL and triglycerides, hypertension, and diabetes)...
  82. Testosterone Replacement Therapy in Advanced Chronic Kidney Disease
    Ralph Rabkin; Fiscal Year: 2009
    ..In 2/3 of males with advanced CKD, serum testosterone (TT) levels are reduced and likely contribute to the wasting...