plasma

Summary

Summary: The residual portion of BLOOD that is left after removal of BLOOD CELLS by CENTRIFUGATION without prior BLOOD COAGULATION.

Top Publications

  1. pmc Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR)
    Evan M Kroh
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Methods 50:298-301. 2010
  2. pmc Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21025, USA
    Retrovirology 9:5. 2012
  3. pmc Potent and broad neutralization of HIV-1 subtype C by plasma antibodies targeting a quaternary epitope including residues in the V2 loop
    Penny L Moore
    National Institute for Communicable Diseases of the NHLS, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 85:3128-41. 2011
  4. doi Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
    Muhammed Murtaza
    Cancer Research UK Cambridge Institute and University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nature 497:108-12. 2013
  5. pmc Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma
    Jason D Arroyo
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Proc Natl Acad Sci U S A 108:5003-8. 2011
  6. doi Detection and characterization of placental microRNAs in maternal plasma
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
    Clin Chem 54:482-90. 2008
  7. ncbi Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions
    P Carmeliet
    The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Leuven, Belgium
    Nat Med 7:575-83. 2001
  8. pmc Kinetics of plasma viremia and soluble nonstructural protein 1 concentrations in dengue: differential effects according to serotype and immune status
    Huynh T L Duyen
    Oxford University Clinical Research Unit, Churchill Hospital, Oxford, UK
    J Infect Dis 203:1292-300. 2011
  9. pmc Comparing the MicroRNA spectrum between serum and plasma
    Kai Wang
    Institute for Systems Biology, Seattle, Washington, United States of America
    PLoS ONE 7:e41561. 2012
  10. pmc Plasma processing conditions substantially influence circulating microRNA biomarker levels
    Heather H Cheng
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS ONE 8:e64795. 2013

Research Grants

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
  2. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
  3. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
  4. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
  5. Ian C Michelow; Fiscal Year: 2016
  6. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
  7. Jill M Wecht; Fiscal Year: 2014
  8. Paolo Fanti; Fiscal Year: 2014
  9. Iron-mediated vascular disease in sickle cell disease.
    John C Wood; Fiscal Year: 2010
  10. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013

Detail Information

Publications387 found, 100 shown here

  1. pmc Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR)
    Evan M Kroh
    Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Methods 50:298-301. 2010
    ..that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases...
  2. pmc Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients
    Kenneth W Witwer
    Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21025, USA
    Retrovirology 9:5. 2012
    ..Using samples from a well-characterized ES cohort, untreated viremic patients, and uninfected controls, we explored the PBMC miRNA profile and probed the relationships of miRNA expression, CD4+ T-cell counts, and viral load...
  3. pmc Potent and broad neutralization of HIV-1 subtype C by plasma antibodies targeting a quaternary epitope including residues in the V2 loop
    Penny L Moore
    National Institute for Communicable Diseases of the NHLS, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 85:3128-41. 2011
    ....
  4. doi Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
    Muhammed Murtaza
    Cancer Research UK Cambridge Institute and University of Cambridge, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nature 497:108-12. 2013
    ..characterized by massively parallel sequencing of circulating cell-free tumour DNA released from cancer cells into plasma, representing a non-invasive liquid biopsy...
  5. pmc Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma
    Jason D Arroyo
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Proc Natl Acad Sci U S A 108:5003-8. 2011
    ..and size-exclusion chromatography as orthogonal approaches to characterize circulating miRNA complexes in human plasma and serum...
  6. doi Detection and characterization of placental microRNAs in maternal plasma
    Stephen S C Chim
    Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, China
    Clin Chem 54:482-90. 2008
    The discovery of circulating fetal nucleic acids in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis...
  7. ncbi Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions
    P Carmeliet
    The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Leuven, Belgium
    Nat Med 7:575-83. 2001
    ..However, loss of PlGF impaired angiogenesis, plasma extravasation and collateral growth during ischemia, inflammation, wound healing and cancer...
  8. pmc Kinetics of plasma viremia and soluble nonstructural protein 1 concentrations in dengue: differential effects according to serotype and immune status
    Huynh T L Duyen
    Oxford University Clinical Research Unit, Churchill Hospital, Oxford, UK
    J Infect Dis 203:1292-300. 2011
    We describe the magnitude and kinetics of plasma viremia and nonstructural protein 1 (sNS1) levels in sequential samples from 167 children with acute dengue, enrolled early in a community study in Vietnam...
  9. pmc Comparing the MicroRNA spectrum between serum and plasma
    Kai Wang
    Institute for Systems Biology, Seattle, Washington, United States of America
    PLoS ONE 7:e41561. 2012
    ..Using serum and corresponding plasma samples from the same individuals, we observed higher miRNA concentrations in serum samples compared to the ..
  10. pmc Plasma processing conditions substantially influence circulating microRNA biomarker levels
    Heather H Cheng
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS ONE 8:e64795. 2013
    ..Our previous work showed that the majority of plasma miRNAs are likely blood cell-derived...
  11. pmc Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages
    Cecilia Lässer
    Krefting Research Centre, Sahlgrenska Academy, University of Gothenburg, Box 424, 405 30 Gothenburg, Sweden
    J Transl Med 9:9. 2011
    ..Our aim was therefore to determine the presence of RNA in exosomes from human saliva, plasma and breast milk and whether these exosomes can be taken up by macrophages.
  12. doi Analysis of the adult human plasma metabolome
    Kay A Lawton
    Metabolon, Inc, 800 Capitola Dr Suite 1, Durham, NC 27713, USA
    Pharmacogenomics 9:383-97. 2008
    ....
  13. ncbi The human plasma proteome: history, character, and diagnostic prospects
    N Leigh Anderson
    Plasma Proteome Institute, Washington, DC 20009 3450, USA
    Mol Cell Proteomics 1:845-67. 2002
    The human plasma proteome holds the promise of a revolution in disease diagnosis and therapeutic monitoring provided that major challenges in proteomics and related disciplines can be addressed...
  14. doi Plasma microRNA-122 as a biomarker for viral-, alcohol-, and chemical-related hepatic diseases
    Yi Zhang
    Department of Medical Genetics, Second Military Medical University, Shanghai, China
    Clin Chem 56:1830-8. 2010
    ..Recently, circulating microRNAs (miRNAs) have been reported as potential biomarkers for the noninvasive diagnosis of cancer. In this study, we investigated whether plasma miRNAs have diagnostic utility in identifying liver disease.
  15. doi Assessing sample and miRNA profile quality in serum and plasma or other biofluids
    Thorarinn Blondal
    Exiqon A S, Skelstedet 16, 2950 Vedbæk, Denmark
    Methods 59:S1-6. 2013
    ..The high relative stability of miRNA in common clinical tissues and biofluids (e.g. plasma, serum, urine, saliva, etc...
  16. pmc A high-confidence human plasma proteome reference set with estimated concentrations in PeptideAtlas
    Terry Farrah
    Institute for Systems Biology, Seattle, WA 98109, USA
    Mol Cell Proteomics 10:M110.006353. 2011
    Human blood plasma can be obtained relatively noninvasively and contains proteins from most, if not all, tissues of the body...
  17. pmc Differences between human plasma and serum metabolite profiles
    Zhonghao Yu
    Research Unit of Molecular Epidemiology, Helmholtz Zentrum Munchen, Neuherberg, Germany
    PLoS ONE 6:e21230. 2011
    Human plasma and serum are widely used matrices in clinical and biological studies. However, different collecting procedures and the coagulation cascade influence concentrations of both proteins and metabolites in these matrices...
  18. ncbi The ratio of blood products transfused affects mortality in patients receiving massive transfusions at a combat support hospital
    Matthew A Borgman
    Brooke Army Medical Center, Fort Sam Houston, TX, USA
    J Trauma 63:805-13. 2007
    ..The risk of death from hemorrhagic shock increases in this population. To treat the coagulopathy of trauma, some have suggested early, aggressive correction using a 1:1 ratio of plasma to red blood cell (RBC) units.
  19. ncbi Calibrated automated thrombin generation measurement in clotting plasma
    H Coenraad Hemker
    Synapse BV, Cardiovascular Research Institute, University of Maastricht, Maastricht, The Netherlands
    Pathophysiol Haemost Thromb 33:4-15. 2003
    Calibrated automated thrombography displays the concentration of thrombin in clotting plasma with or without platelets (platelet-rich plasma/platelet-poor plasma, PRP/PPP) in up to 48 samples by monitoring the splitting of a fluorogenic ..
  20. ncbi Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC(FL))) of plasma and other biological and food samples
    Ronald L Prior
    U S Department of Agriculture, Agriculture Research Service, Arkansas, Children s Nutrition Center, 1120 Marshall Street, Little Rock, Arkansas 72202, USA
    J Agric Food Chem 51:3273-9. 2003
    ..provide, for the first time, the ability to obtain a measure of "total antioxidant capacity" in the protein free plasma, using the same peroxyl radical generator for both lipophilic and hydrophilic antioxidants...
  21. doi iTRAQ experimental design for plasma biomarker discovery
    Xiaomin Song
    Australian Proteome Analysis Facility Ltd, Macquarie University, Sydney, Australia
    J Proteome Res 7:2952-8. 2008
    There is considerable interest in using mass spectrometry for biomarker discovery in human blood plasma. We investigated aspects of experimental design for large studies that require analysis of multiple sample sets using iTRAQ reagents ..
  22. doi Improved detection of CXCR4-using HIV by V3 genotyping: application of population-based and "deep" sequencing to plasma RNA and proviral DNA
    Luke C Swenson
    British Columbia Centre for Excellence in HIV AIDS, Vancouver, Canada
    J Acquir Immune Defic Syndr 54:506-10. 2010
    ..Tropism testing should rule out CXCR4-using HIV before treatment with CCR5 antagonists. Currently, the recombinant phenotypic Trofile assay (Monogram) is most widely utilized; however, genotypic tests may represent alternative methods...
  23. pmc Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma
    Andrey Turchinovich
    Molecular Epidemiology C080, German Cancer Research Center, Heidelberg, Germany
    RNA Biol 9:1066-75. 2012
    ..Additionally, we show that circulating miRNA in human blood plasma can be immunoprecipitated with both AGO1 and AGO2 antibody...
  24. ncbi Plasma antioxidants are similarly depleted in mild cognitive impairment and in Alzheimer's disease
    P Rinaldi
    Department of Gerontology and Geriatrics, University of Perugia, Policlinico Monteluce Pad E, Via Brunamonti 51, 06126 Perugia, Italy
    Neurobiol Aging 24:915-9. 2003
    ..and enzymatic antioxidants in elderly subjects with mild cognitive impairment (MCI) and Alzheimer's disease (AD), plasma levels of water-soluble (Vitamin C and uric acid) and of lipophilic (Vitamin A, Vitamin E and carotenoids ..
  25. pmc The complex exogenous RNA spectra in human plasma: an interface with human gut biota?
    Kai Wang
    Institute for Systems Biology, Seattle, Washington, United States of America
    PLoS ONE 7:e51009. 2012
    Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers...
  26. ncbi Stability of endogenous and added RNA in blood specimens, serum, and plasma
    Nancy B Y Tsui
    Department of Chemical Pathology, Sir Y K Pao Cancer Center, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region
    Clin Chem 48:1647-53. 2002
    Circulating RNA in plasma/serum is an emerging field for noninvasive molecular diagnosis...
  27. doi Discordance between cerebral spinal fluid and plasma HIV replication in patients with neurological symptoms who are receiving suppressive antiretroviral therapy
    Ana Canestri
    Hopital Pitie Salpetriere, Paris, France
    Clin Infect Dis 50:773-8. 2010
    We report data on 11 patients with neurological symptoms and human immunodeficiency virus (HIV) cerebrospinal fluid (CSF) viremia contrasting with suppressed plasma HIV RNA during receipt of combined antiretroviral therapy.
  28. ncbi Treatment with convalescent plasma for influenza A (H5N1) infection
    Boping Zhou
    N Engl J Med 357:1450-1. 2007
  29. ncbi Plasma terminal half-life
    P L Toutain
    UMR 181 Physiopathologie et Toxicologie Experimentales INRA ENVT, Ecole Nationale Veterinaire de Toulouse, Toulouse cedex 03, France
    J Vet Pharmacol Ther 27:427-39. 2004
    Terminal plasma half-life is the time required to divide the plasma concentration by two after reaching pseudo-equilibrium, and not the time required to eliminate half the administered dose...
  30. doi A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma
    Ryan Grant
    Cellular and Molecular Immunology Research Centre, Faculty of Life Sciences, London Metropolitan University, London, UK
    J Immunol Methods 371:143-51. 2011
    The methods of Plasma Membrane-derived Vesicle (PMV) isolation and quantification vary considerably in the literature and a new standard needs to be defined...
  31. doi The procoagulant and proinflammatory plasma contact system
    Thomas Renne
    Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital Solna, Stockholm, Sweden
    Semin Immunopathol 34:31-41. 2012
    The contact system is a plasma protease cascade that is initiated by coagulation factor XII activation on cardiovascular cells...
  32. pmc Characterization of human plasma-derived exosomal RNAs by deep sequencing
    Xiaoyi Huang
    Department of Pathology and Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 14:319. 2013
    ..To characterize exosomal RNA profiles systemically, we performed RNA sequencing analysis using three human plasma samples and evaluated the efficacies of small RNA library preparation protocols from three manufacturers...
  33. pmc The diagnostic accuracy of pleural effusion and plasma samples versus tumour tissue for detection of EGFR mutation in patients with advanced non-small cell lung cancer: comparison of methodologies
    Xiaoqing Liu
    Affiliated Hospital of Academy of Military Medical Science, Beijing, China
    J Clin Pathol 66:1065-9. 2013
    To evaluate the suitability of malignant pleural effusion (MPE) and plasma as surrogate samples for epidermal growth factor receptor (EGFR) mutation detection, and compare three different detection methods.
  34. ncbi Sphingosine 1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum
    Y Yatomi
    Department of Laboratory Medicine, Yamanashi Medical University, Nakakoma gun
    J Biochem 121:969-73. 1997
    ..In this study, we identified Sph-1-P as a normal constituent of human plasma and serum...
  35. pmc A coordinated change in chemokine responsiveness guides plasma cell movements
    D C Hargreaves
    Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA
    J Exp Med 194:45-56. 2001
    Antibody-secreting plasma cells are nonrecirculatory and lodge in splenic red pulp, lymph node medullary cords, and bone marrow. The factors that regulate plasma cell localization are poorly defined...
  36. doi Proteomic analysis of parasitized Plutella xylostella larvae plasma
    Kyung Han Song
    School of Life Science and Biotechnology, Korea University, Seoul 136 701, Republic of Korea
    J Insect Physiol 54:1270-80. 2008
    ..Using 2D polyacrylamide gel electrophoresis, proteins in the host plasma were assessed every 48 h after parasitism by C. plutellae...
  37. pmc Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model
    Marguerite R Kelher
    Bonfils Blood Center, Denver, CO 80230, USA
    Blood 113:2079-87. 2009
    ..1) the clinical condition of the patient and (2) the infusion of antibodies against MHC class I antigens or the plasma from stored blood...
  38. doi Cell-free tumor DNA in blood plasma as a marker for circulating tumor cells in prostate cancer
    Heidi Schwarzenbach
    Institute of Tumor Biology, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Clin Cancer Res 15:1032-8. 2009
    ..Circulating cell-free DNA in the blood of cancer patients harbors tumor-specific aberrations. Here, we investigated whether this DNA might also reflect the presence of circulating tumor cells (CTC)...
  39. doi Plasma leakage in dengue haemorrhagic fever
    Anon Srikiatkhachorn
    University of Massachusetts Medical School, Center for Infectious Diseases and Vaccine Research, Worcester, MA 01655 0002, USA
    Thromb Haemost 102:1042-9. 2009
    ..disease including dengue haemorrhagic fever (DHF), a viral haemorrhagic disease characterised by bleeding and plasma leakage. The characteristic feature of DHF is the transient period of plasma leakage and a haemorrhagic tendency...
  40. doi The effect of heat- or ultra violet ozone-treatment of titanium on complement deposition from human blood plasma
    Paula Linderbäck
    Division of Applied Physics, Department of Physics and Measurement Technology, Linkoping University, SE 581 83 Linkoping, Sweden
    Biomaterials 31:4795-801. 2010
    ..It integrates well to bone and soft tissues, and is shown upon blood plasma contact to activate the intrinsic pathway of coagulation and bind complement factor 3b...
  41. doi Payment, compensation and replacement--the ethics and motivation of blood and plasma donation
    A Farrugia
    Plasma Protein Therapeutics Association, Annapolis, MD 21401, USA
    Vox Sang 99:202-11. 2010
    The supply of blood and plasma to produce haemotherapies varies around the world, but all environments need donors to furnish the raw material...
  42. doi Plasma Plasmodium falciparum histidine-rich protein-2 concentrations do not reflect severity of malaria in Papua new guinean children
    Laurens Manning
    School of Medicine and Pharmacology, University of Western Australia, Fremantle Hospital, Western Australia
    Clin Infect Dis 52:440-6. 2011
    In areas of unstable malaria transmission, plasma Plasmodium falciparum histidine-rich protein 2 (PfHRP-2) concentrations parallel total parasite biomass and thus infection severity...
  43. pmc HIV-specific functional antibody responses in breast milk mirror those in plasma and are primarily mediated by IgG antibodies
    Genevieve G Fouda
    Duke Human Vaccine Institute, Duke University Medical Center, Box 103020, Durham, NC 27710, USA
    J Virol 85:9555-67. 2011
    ..virus neutralization, and antibody-dependent cell cytotoxicity (ADCC) responses were measured in the milk and plasma of 41 HIV-infected lactating women...
  44. pmc Plasma components affect accuracy of circulating cancer-related microRNA quantitation
    Dong Ja Kim
    Department of Pathology, Chicago Medical School, North Chicago, Illinois 60064, USA
    J Mol Diagn 14:71-80. 2012
    ..Overall, the present study outlines key factors that prevent accurate miRNA quantitation in body fluids and provides approaches that enable faithful quantitation of miRNA abundance in body fluids...
  45. pmc Clinical review: Fresh frozen plasma in massive bleedings - more questions than answers
    Bartolomeu Nascimento
    Transfusion Medicine, Sunnybrook Health Sciences Centre, University of Toronto, 2075 Bayview Ave, C160, Toronto, ON M4N 3M5, Canada
    Crit Care 14:202. 2010
    Fresh frozen plasma (FFP) is indicated for the management of massive bleedings. Recent audits suggest physician knowledge of FFP is inadequate and half of the FFP transfused in critical care is inappropriate...
  46. doi Cell-free plasma DNA as a predictor of outcome in severe sepsis and septic shock
    Katri Saukkonen
    Departments of Medicine and Emergency Care, Helsinki University Central Hospital, Helsinki, Finland
    Clin Chem 54:1000-7. 2008
    Increased concentrations of cell-free DNA have been found in plasma of septic and critically ill patients...
  47. ncbi Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration
    G Weibrich
    Department of Prosthetics, J Gutenberg University, Augustusplatz 2, Mainz 55131, Germany
    Bone 34:665-71. 2004
    This study analyzed the effect of the platelet count in platelet-rich plasma (PRP) on bone regeneration in vivo. Twenty male New Zealand white rabbits were used...
  48. ncbi Incorporation and clearance of omega-3 fatty acids in erythrocyte membranes and plasma phospholipids
    Jing Cao
    University of Minnesota, Minneapolis, MN 55455, USA
    Clin Chem 52:2265-72. 2006
    ..To study the effects of fatty acid supplementation, we investigated the rate of incorporation and clearance of these fatty acids in erythrocyte membranes and plasma after intake of supplements.
  49. doi Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines
    Irundika H K Dias
    School of Life and Health Sciences, Aston University, Birmingham, UK
    J Clin Periodontol 38:1-7. 2011
    To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines.
  50. doi Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry
    Warwick B Dunn
    Manchester Centre for Integrative Systems Biology, Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, UK
    Nat Protoc 6:1060-83. 2011
    ..Protocols for serum- and plasma-based metabolic profiling applying gas chromatography-MS (GC-MS) and ultraperformance liquid chromatography-MS (..
  51. ncbi Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis--a preliminary result of three cases
    Hiroshi Kitoh
    Department of Orthopaedic Surgery, Nagoya University School of Medicine, Showa Ku, Nagoya, Aichi 466 8550, Japan
    Bone 35:892-8. 2004
    ..of distraction osteogenesis with transplantation of marrow-derived mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) were reviewed in three femora and two tibiae of the two patients with achondroplasia and one patient with ..
  52. pmc Scarcity or absence of humoral immune responses in the plasma and cervicovaginal lavage fluids of heavily HIV-1-exposed but persistently seronegative women
    Jiri Mestecky
    Departments of Microbiology and Medicine, University of Alabama at Birmingham, USA
    AIDS Res Hum Retroviruses 27:469-86. 2011
    ..the female genital tract secretions of highly-exposed but persistently seronegative (HEPSN) women, 41 samples of plasma and cervicovaginal lavage (CVL) fluid were distributed to six laboratories for their blinded evaluation using ..
  53. pmc Transfusion in trauma: thromboelastometry-guided coagulation factor concentrate-based therapy versus standard fresh frozen plasma-based therapy
    Herbert Schöchl
    Ludwig Boltzmann Institute of Experimental and Clinical Traumatology, Donaueschingenstrasse 13, A 1200 Vienna, Austria
    Crit Care 15:R83. 2011
    ..fibrinogen concentrate and prothrombin complex concentrate (PCC) in trauma patients may reduce the need for transfusion of red blood cells (RBC) or platelet concentrate, compared with fresh frozen plasma (FFP)-based haemostatic therapy.
  54. ncbi Size distributions of maternal and fetal DNA in maternal plasma
    K C Allen Chan
    Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China
    Clin Chem 50:88-92. 2004
    The discovery of fetal DNA in maternal plasma has opened up an approach for noninvasive prenatal diagnosis. Despite the rapid expansion in clinical applications, the molecular characteristics of plasma DNA in pregnant women remain unclear.
  55. ncbi Effect of repeated freeze-thaw cycles on routine plasma biochemical constituents in canine plasma
    Brice Reynolds
    UMR 181 de Physiopathologie et Département des Sciences Cliniques, Ecole Nationale Veterinaire de Toulouse, Toulouse, France
    Vet Clin Pathol 35:339-40. 2006
    The effect of repeated freeze-thaw cycles on plasma constituents has not been assessed in dogs, although such a procedure is not uncommon to use in routine laboratory practice.
  56. ncbi Circulating (cell-free) nucleic acids--a promising, non-invasive tool for early detection of several human diseases
    Vishnu Swarup
    Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India
    FEBS Lett 581:795-9. 2007
    Circulating nucleic acids (CNA) are present in small amounts in the plasma of healthy individuals...
  57. ncbi The effect of positively charged plasma polymerization on initial osteoblastic focal adhesion on titanium surfaces
    Birgit Finke
    Institute of Low Temperature Plasma Physics INP, Felix Hausdorff Strasse 2, 17489 Greifswald, Germany
    Biomaterials 28:4521-34. 2007
    ..Here, titanium was coated with a very thin, adherent, cross-linked, pinhole- and additive-free allylamine plasma polymer layer (PPAAm) resistant to hydrolysis and delamination and equipped with a high density of positively ..
  58. doi Differential scanning calorimetry of blood plasma for clinical diagnosis and monitoring
    Nichola C Garbett
    James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
    Exp Mol Pathol 86:186-91. 2009
    Differential scanning calorimetry (DSC) provides a useful method to study the unfractionated plasma proteome...
  59. doi Plasma total nitric oxide and endothelial constitutive nitric oxide synthase (ecNOS) gene polymorphism: a study in a South Indian population
    Priya Gururajan
    Department of Biochemistry, International Centre for Cardiothoracic and Vascular Diseases, Dr K M Cherian Heart Foundation A Unit of Frontier LifeLine Pvt Ltd, R 30 C Ambattur Industrial Estate Road, Mogaippair, Chennai, India
    Biochem Genet 49:96-103. 2011
    ..of the possible association of endothelial constitutive nitric oxide synthase (ecNOS) gene polymorphism and plasma nitric oxide levels in patients with acute coronary syndrome, we investigated 106 patients with the syndrome and ..
  60. doi Plasma versus serum levels of osteopontin and mesothelin in patients with malignant mesothelioma--which is best?
    Jenette Creaney
    National Research Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Sir Charles Gairdner Hospital, 4th Floor, G Block, Verdun St, Nedlands, WA 6009, Australia
    Lung Cancer 74:55-60. 2011
    ..As there is some concern about the sensitivity of osteopontin in serum versus plasma, we compared them in the same patient population to mesothelin.
  61. pmc Genotypic prediction of human immunodeficiency virus type 1 tropism by use of plasma and peripheral blood mononuclear cells in the routine clinical laboratory
    Christian Paar
    Institute of Laboratory Medicine1 and Department of Dermatology, 2 General Hospital Linz, Krankenhausstrasse 9, A 4020 Linz, Austria
    J Clin Microbiol 49:2697-9. 2011
    ..The assay allows examination of HIV RNA from plasma and HIV DNA from peripheral blood mononuclear cells (PBMC), including PBMC samples from patients with undetectable ..
  62. pmc Validation for clinical use of a novel HIV-2 plasma RNA viral load assay using the Abbott m2000 platform
    Ming Chang
    Department of Laboratory Medicine, Division of Virology, University of Washington, Seattle, United States
    J Clin Virol 55:128-33. 2012
    Optimal care of persons infected with human immunodeficiency virus type 2 (HIV-2) requires an accurate assessment of HIV-2 plasma viral load (VL), but no clinically approved quantitative HIV-2 RNA VL assay exists.
  63. doi Calorimetry-based profiling of blood plasma from colorectal cancer patients
    Svetla Todinova
    Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria
    Biochim Biophys Acta 1820:1879-85. 2012
    ..To further elaborate this approach we have applied DSC in a study of blood plasma from patients with colorectal cancer (CRC) at different stages of tumor development and localization.
  64. pmc A single-nucleotide polymorphism in CYP2B6 leads to >3-fold increases in efavirenz concentrations in plasma and hair among HIV-infected women
    Monica Gandhi
    Department of Medicine, University of California, San Francisco, CA, USA
    J Infect Dis 206:1453-61. 2012
    Efavirenz exhibits marked interindividual variability in plasma levels and toxicities...
  65. pmc Measurement of thyroglobulin by liquid chromatography-tandem mass spectrometry in serum and plasma in the presence of antithyroglobulin autoantibodies
    Mark M Kushnir
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 59:982-90. 2013
    ..Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immunoassays (IAs) and cause false-negative results...
  66. pmc Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep
    Sabine B Glor
    Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 266a, Zurich CH 8057, Switzerland
    Parasit Vectors 6:85. 2013
    ..Among food animals, sheep along with goats and pigs possess the highest incidence of T. gondii cysts in meat, and play a major role as a source of human infection...
  67. ncbi Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin
    Sara G Llames
    Centro Comunitario de Sangre y Tejidos del Principado de Asturias, Emilio Rodriguez Vigil s n, Oviedo, Spain
    Transplantation 77:350-5. 2004
    ..on (1) keratinocytes and fibroblasts obtained from a single skin biopsy and (2) a dermal matrix based on human plasma. A high expansion capacity achieved by keratinocytes grown on this plasma-based matrix is reported...
  68. ncbi Comparison between plasma and erythrocyte fatty acid content as biomarkers of fatty acid intake in US women
    Qi Sun
    Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA
    Am J Clin Nutr 86:74-81. 2007
    Erythrocyte fatty acids may be superior to plasma fatty acids for reflecting long-term fatty acid intake because of less sensitivity to recent intake and a slower turnover rate.
  69. ncbi Interrogation of the plasma proteome with differential scanning calorimetry
    Nichola C Garbett
    James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40292, USA
    Clin Chem 53:2012-4. 2007
  70. doi Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser light
    Shangyuan Feng
    Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China
    Biosens Bioelectron 26:3167-74. 2011
    We have recently applied surface-enhanced Raman spectroscopy (SERS) for blood plasma analysis for non-invasive nasopharyngeal cancer detection and obtained good preliminary results...
  71. ncbi Saliva parameters as potential indices of hydration status during acute dehydration
    Neil P Walsh
    School of Sport, Health and Exercise Sciences, University of Wales, Bangor, United Kingdom
    Med Sci Sports Exerc 36:1535-42. 2004
    ..and total protein are potential markers of hydration, we compared changes in these parameters with changes in plasma osmolality during progressive dehydration...
  72. ncbi Autologous plasma activates Akt/protein kinase B and enhances basal survival and resistance to DNA damage-induced apoptosis in B-chronic lymphocytic leukaemia cells
    R G Wickremasinghe
    Department of Haematology, Royal Free and University College School of Medicine, London, UK
    Br J Haematol 114:608-15. 2001
    We have studied the actions of autologous plasma on both basal and DNA damage-induced apoptosis in B-chronic lymphocytic leukaemia (B-CLL) cells...
  73. ncbi Chemotactic response of Helicobacter pylori to human plasma and bile
    Mulugeta L Worku
    Department of Medical Microbiology, Imperial College School of Medicine at St Mary s, Norfolk Place, London W2 1NY, UK
    J Med Microbiol 53:807-11. 2004
    ..further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined...
  74. pmc Peroxidase activity of hemoglobin-haptoglobin complexes: covalent aggregation and oxidative stress in plasma and macrophages
    Alexandr Kapralov
    Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
    J Biol Chem 284:30395-407. 2009
    ..vi) Hb-Hp aggregates cause cytotoxicity to macrophages; and (vii) Hb-Hp aggregates are present in septic plasma. Overall, our data suggest that under conditions of severe inflammation and oxidative stress, peroxidase activity ..
  75. doi Potential clinical significance of a plasma-based KRAS mutation analysis in patients with advanced non-small cell lung cancer
    Shuhang Wang
    Key Laboratory of Carcinogenesis and Translational Research Ministry of Education, Department of Thoracic Medical Oncology, Peking University School of Oncology, Beijing Cancer Hospital and Institute, Beijing, China
    Clin Cancer Res 16:1324-30. 2010
    ..This study aims to evaluate a plasma-based KRAS mutation analysis and the clinical significance of plasma KRAS mutation as a predictive marker for ..
  76. doi Plasma membrane microdomains in aging and disease
    Yoshiko Ohno-Iwashita
    Tokyo Metropolitan Institute of Gerontology, Itabashi ku, Tokyo, Japan
    Geriatr Gerontol Int 10:S41-52. 2010
    The plasma membrane of eukaryotic cells participates in signal transduction and many other cellular events to maintain the physiological state of cells...
  77. doi Stand-alone self-powered integrated microfluidic blood analysis system (SIMBAS)
    Ivan K Dimov
    Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland
    Lab Chip 11:845-50. 2011
    ..white cells, without external valving or pumping mechanisms, followed by analyte detection in platelet-containing plasma. Five complete biotin-streptavidin sample-to-answer assays are performed in 10 min; the limit of detection is 1...
  78. doi Measurement of human plasma proteome dynamics with (2)H(2)O and liquid chromatography tandem mass spectrometry
    John C Price
    KineMed, Emeryville, CA 94608, USA
    Anal Biochem 420:73-83. 2012
    ..4 to 40 days were measured using 8 μl of plasma. The measured rates were consistent with literature values...
  79. doi A lectin-coupled, targeted proteomic mass spectrometry (MRM MS) platform for identification of multiple liver cancer biomarkers in human plasma
    Yeong Hee Ahn
    Division of Mass Spectrometry, Korea Basic Science Institute, Cheongwon Gun 363 883, Republic of Korea
    J Proteomics 75:5507-15. 2012
    ..proteins related to liver cancer progression were captured with specific lectin and identified from human plasma by multiple reaction monitoring (MRM) mass spectrometry as multiple biomarkers for hepatocellular carcinoma (HCC)...
  80. ncbi Higher amount of free circulating DNA in serum than in plasma is not mainly caused by contaminated extraneous DNA during separation
    Naoyuki Umetani
    Department of Molecular Oncology, John Wayne Cancer Institute, 2200 Santa Monica Blvd, Santa Monica, CA 90404, USA
    Ann N Y Acad Sci 1075:299-307. 2006
    Circulating DNA isolated from serum and plasma has been shown to be a useful biomarker in various diseases including cancer. Serum reportedly contains a higher amount of free circulating DNA than it does in plasma...
  81. doi Differences in HIV type 1 RNA plasma load profile of closely related cocirculating Ethiopian subtype C strains: C and C'
    Workenesh Ayele
    Ethiopian Health and Nutrition Research Institute EHNRI, Addis Ababa, Ethiopia
    AIDS Res Hum Retroviruses 26:805-13. 2010
    ..infection with subcluster C viruses leads to a more rapid onset of disease, despite the initial lower HIV-1 RNA plasma loads...
  82. pmc Pharmacokinetics of artemether and dihydroartemisinin in healthy Pakistani male volunteers treated with artemether-lumefantrine
    Shabana Ali
    Department of Pharmacology and Therapeutics, Army Medical College, National University of Sciences and Technology NUST, Islamabad, Pakistan
    Malar J 9:275. 2010
    ..The aim of this study was to evaluate the pharmacokinetics of artemether and its active metabolite, dihydroartemisinin, in healthy Pakistani volunteers...
  83. pmc Streptococcus pyogenes in human plasma: adaptive mechanisms analyzed by mass spectrometry-based proteomics
    Johan Malmstrom
    Department of Immunotechnology, Lund University, SE 22100 Lund, Sweden
    J Biol Chem 287:1415-25. 2012
    Streptococcus pyogenes is a major bacterial pathogen and a potent inducer of inflammation causing plasma leakage at the site of infection...
  84. ncbi Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms
    Ying Li
    University Women s Hospital Department of Research, University of Basel, Basel, Switzerland
    Clin Chem 50:1002-11. 2004
    Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g...
  85. ncbi Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma
    Lynn A Echan
    The Wistar Institute, Philadelphia, PA 19104, USA
    Proteomics 5:3292-303. 2005
    ..Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently commercialized HPLC column containing antibodies to six of the most abundant blood ..
  86. ncbi Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, the Sanquin Blood Bank South East, the Department of Health Risk Analysis and Toxicology NUTRIM, Maastricht University, Maastricht, The Netherlands
    Transfusion 46:1018-28. 2006
    ..In vivo, this is antagonized by ectonucleotidases on the surface of endothelial cells and white blood cells (WBCs). The receptor desensitization of ATP- and ADP-induced responses of PLTs stored in plasma without WBCs was investigated.
  87. ncbi A data base for partition of volatile organic compounds and drugs from blood/plasma/serum to brain, and an LFER analysis of the data
    Michael H Abraham
    Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK
    J Pharm Sci 95:2091-100. 2006
    Literature values of the in vivo distribution (BB) of drugs from blood, plasma, or serum to rat brain have been assembled for 207 compounds (233 data points)...
  88. pmc Relationship between residual plasma viremia and the size of HIV proviral DNA reservoirs in infected individuals receiving effective antiretroviral therapy
    Tae Wook Chun
    Laboratory of Immunoregulation, National Institutes of Health, Bethesda, MD, USA
    J Infect Dis 204:135-8. 2011
    Residual plasma viremia (<50 copies/mL) persists in certain human immunodeficiency virus (HIV)-infected individuals receiving antiretroviral therapy (ART); however, the relationship between the degree of residual plasma viremia, the ..
  89. ncbi Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing
    Barry L Eppley
    Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis 46202, USA
    Plast Reconstr Surg 114:1502-8. 2004
    ..recent strategy to promote the wound-healing cascade is to prepare an autologous platelet concentrate suspended in plasma, also known as platelet-rich plasma, that contains growth factors and administer it to wound sites...
  90. ncbi Platelet-rich plasma stimulates porcine articular chondrocyte proliferation and matrix biosynthesis
    K Akeda
    Department of Orthopedic Surgery, Rush Medical College at Rush University Medical Center, Chicago, IL 60612, USA
    Osteoarthritis Cartilage 14:1272-80. 2006
    Platelet-rich plasma (PRP) is a fraction of plasma that contains high levels of multiple growth factors...
  91. ncbi Serum and plasma proteomics
    Haleem J Issaq
    Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC Frederick, Inc, NCI Frederick, P O Box B, Frederick, Maryland 21702, USA
    Chem Rev 107:3601-20. 2007
  92. ncbi Oxidative stress parameters in plasma of Huntington's disease patients, asymptomatic Huntington's disease gene carriers and healthy subjects : a cross-sectional study
    N Klepac
    Dept of Neurology, University Clinical Hospital Center Zagreb, Zagreb University School of Medicine, Kispaticeva, Zagreb, Croatia
    J Neurol 254:1676-83. 2007
    ..Animal data and postmortem studies suggest a role of oxidative stress in the Huntington's disease (HD), but in vivo human studies have been scarce...
  93. doi icoshift: A versatile tool for the rapid alignment of 1D NMR spectra
    F Savorani
    Quality and Technology, Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, 1958 Frederiksberg C, Denmark
    J Magn Reson 202:190-202. 2010
    ..The algorithm is made open source and the Matlab code including documentation can be downloaded from www.models.life.ku.dk...
  94. pmc Genetic diversity of simian immunodeficiency virus encoding HIV-1 reverse transcriptase persists in macaques despite antiretroviral therapy
    Mary Kearney
    HIV Drug Resistance Program, National Cancer Institute at Frederick, 1050 Boyles Street, Building 535, Room 109, Frederick, MD 21702 1201, USA
    J Virol 85:1067-76. 2011
    ..All macaques received 20 weeks of tenofovir, emtricitabine, and EFV. Plasma virus populations were analyzed by single-genome sequencing...
  95. doi Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona: a comprehensive quantitative proteomic analysis
    Stefan Tenzer
    Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany
    ACS Nano 5:7155-67. 2011
    ..We studied the long-lived ("hard") blood plasma derived corona on monodispersed amorphous silica nanoparticles differing in size (20, 30, and 100 nm)...
  96. pmc Increased plasma soluble endoglin levels as an indicator of cardiovascular alterations in hypertensive and diabetic patients
    Ana M Blázquez-Medela
    Unidad de Fisiopatología Renal y Cardiovascular, Instituto Reina Sofia de Investigacion Nefrologica, Universidad de Salamanca, Salamanca, Spain
    BMC Med 8:86. 2010
    ..Thus, we studied the relationship between plasma levels of soluble endoglin and cardiovascular alterations associated with hypertension and diabetes.
  97. ncbi Relevance of apple polyphenols as antioxidants in human plasma: contrasting in vitro and in vivo effects
    Silvina B Lotito
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA
    Free Radic Biol Med 36:201-11. 2004
    ..contribution to the total antioxidant capacity of apple extracts; (2) the effects of adding apple extract to human plasma in vitro on oxidation of endogenous antioxidants and lipids; and (3) the effects of apple consumption by humans on ..
  98. ncbi Age-associated analysis of oxidative stress parameters in human plasma and erythrocytes
    Lizette Gil
    Hospital of the Institute Pedro Kourí, Department of Clinical Pharmacology, Havana, Cuba
    Free Radic Res 40:495-505. 2006
    ..The purpose of this study was to examine the redox status in plasma and erythrocytes of healthy individuals and determine correlations between these parameters and the aging process...
  99. doi Angiotensin-converting enzyme 2 catalytic activity in human plasma is masked by an endogenous inhibitor
    Rebecca A Lew
    Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Clayton, Victoria 3800, Australia
    Exp Physiol 93:685-93. 2008
    ..Both ACE and ACE2 are membrane-bound ectoenzymes and may circulate in plasma as a consequence of a proteolytic shedding event...
  100. doi Ten years of hemovigilance reports of transfusion-related acute lung injury in the United Kingdom and the impact of preferential use of male donor plasma
    Catherine E Chapman
    NHS Blood and Transplant, Newcastle upon Tyne, UK
    Transfusion 49:440-52. 2009
    ..From late 2003, the National Blood Service provided 80 to 90 percent of fresh-frozen plasma (FFP) and plasma for platelet (PLT) pools from male donors.
  101. doi Hemostatic effects of fresh frozen plasma may be maximal at red cell ratios of 1:2
    Ross Davenport
    Trauma Clinical Academic Unit, Blizard Institute of Cell and Molecular Science, The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom
    J Trauma 70:90-5; discussion 95-6. 2011
    ..control resuscitation targets acute traumatic coagulopathy with the early administration of high-dose fresh frozen plasma (FFP). FFP is administered empirically and as a ratio with the number of packed red blood cells (PRBC)...

Research Grants81

  1. Discovery metabolite profiling of the prolyl peptidases
    Alan Saghatelian; Fiscal Year: 2009
    ..Furthermore, the application of DMP to peptidases will demonstrate the generality of this approach for the future characterization of medically relevant enzymes and signaling pathways. ..
  2. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  3. CFI Pathogen Inactivation Technology
    Trevor P Castor; Fiscal Year: 2010
    ..in the healthcare community -- the need for effective pathogen inactivation and removal techniques for human blood plasma and plasma-derived products...
  4. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..microscopy techniques and lipidomics to show that IgE receptors localize to distinct microdomains in the plasma membrane during signaling and receptor internalization...
  5. Ian C Michelow; Fiscal Year: 2016
    ..Dr. Michelow's team will capitalize on plasma samples and epidemiological data that they already collected from a large well-characterized longitudinal cohort ..
  6. Cardiotrophin-Like Cytokine 1, a Candidate Molecule for the FSGS Factor
    Virginia J Savin; Fiscal Year: 2010
    ..We have documented that serum or plasma from FSGS patients increases glomerular albumin permeability (Palb) and that the specific activity of certain ..
  7. Jill M Wecht; Fiscal Year: 2014
    ..in urinary markers of catecholamine release (vanillyl mandelic acid, metanephrines and normetanephrine), and in plasma levels of atrial natriuretic factor, a marker of atrial stretch...
  8. Paolo Fanti; Fiscal Year: 2014
    ..e. markers of renal inflammation. In plasma and in peripheral blood monocytes from the same patients, we will analyze GSH content and activity of GSH ..
  9. Iron-mediated vascular disease in sickle cell disease.
    John C Wood; Fiscal Year: 2010
    ..Iron-mediated oxidative stress and increased levels of labile plasma iron (LPI), may worsen intravascular hemolysis and impair endothelial function, as clearly seen in the thalassemia ..
  10. Role of Chromogranin A in Metabolic Syndrome
    Sushil K Mahata; Fiscal Year: 2013
    ..of Chga in metabolic disorder, we have generated Chga knockout mice (Chga-KO), which display hypertension, high plasma catecholamines, increased hepatic sensitivity to insulin and muscle insulin resistance...
  11. The gastric X/A-like (ghrelin) cell: A peripheral regulator of food intake
    NILS WG LAMBRECHT; Fiscal Year: 2012
    ..and re-feeding on (a) nesfatin-1/NUCB2 concentration and ghrelin/desacyl ghrelin (DAG) ratios in rat blood plasma and on (b) mRNA and protein expression of ghrelin, GOAT, and nesfatin-1/NUCB2 in enriched rat oxyntic mucosal ..
  12. Noreen F Rossi; Fiscal Year: 2016
    ..as well as directly to the kidney;and (3) We will determine the effect of exercise trainin on renin release, plasma and renal tissue Ang II and renal sodium excretion via the distal sodium chloride co-transporter (NCC) and ..
  13. VINCENT SCOTT TAGLIABRACCI; Fiscal Year: 2014
    ..x is any amino acid and Glu/pSer can be Glu or phospho-Ser) and this motif is phosphorylated in some 75% of human plasma and cerebrospinal fluid phosphoproteins...
  14. Comprehensive biomarker study to capitalize on existing GWAS in 10K South Asians
    Danish Saleheen; Fiscal Year: 2010
    ..We seek support to add a comprehensive biochemical dimension to PROMIS by assaying plasma biomarkers potentially relevant to atherogenesis, plaque rupture and/or thrombosis...
  15. Importance of antigen specific immunoglobulin responses in controlling SIV/SHIV i
    Bapi Pahar; Fiscal Year: 2010
    ..We will also compare quantitative and qualitative binding properties of all longitudinal plasma/serum samples and/or mucosal secretions from macaques with different levels of immunity to trimeric recombinant ..
  16. Effective treatment of sleep apnea in prediabetes to reduce cardiometabolic risk
    Esra Tasali; Fiscal Year: 2010
    ..Secondary outcomes will be plasma levels of catecholamines, free fatty acids, leptin and CRP...
  17. Prostate, Lung, Colorectal and Ovarian (PLCO) Screening Trial
    BARBARA O'BRIEN; Fiscal Year: 2013
    ..Whole blood, sequential serum samples and one plasma sample were collected from the intervention arm...
  18. Prostate, Lung, Colorectal and Ovarian (PLCO) Screening Trial
    Mona Fouad; Fiscal Year: 2013
    ..Whole blood, sequential serum samples and one plasma sample were collected from the intervention arm...
  19. Peroxynitrite Decomposition Catalyst and Nitric Oxide Donor for Endotoxemia
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..Tissue levels of R-100 and metabolites from heart, lung, kidney, and liver will be measured, in order to relate plasma and organ drug uptake...
  20. Ashraf Gorgey; Fiscal Year: 2016
    ..The favorable adaptations in body composition were associated with decrease in plasma insulin area under the curve and plasma triglycerides...
  21. Merrill D Benson; Fiscal Year: 2014
    ..SAA, an apolipoprotein of plasma HDL, is the precursor of the amyloid fibril subunit AA...
  22. Priyabrata Mukherjee; Fiscal Year: 2016
    ..HHcy, an elevation of plasma Hcy level, is an independent risk factor for cardiovascular (CVD) and neurovascular diseases (NVDs)...
  23. VINCENT COLLINS OFUKA NJAR; Fiscal Year: 2014
    ..prospective study of 208 postmenopausal women operated on for breast cancer, it was recently reported that low plasma retinol strongly predicts poorer prognosis in patients...
  24. Jonathan Savitz; Fiscal Year: 2016
    ..My aim for the proposed K01 is to examine the association between the plasma concentrations of IL6, IL10, TRP/KYN metabolites, and three neurophysiological correlates of MDD: (a) the abnormal ..
  25. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
    B cells respond to environmental antigens by assembling signalosomes at the plasma membrane for signal transduction...
  26. REGULATION AND FUNCTION OF STE20-RELATED PROTEIN KINASES
    Melanie H Cobb; Fiscal Year: 2012
    ..vesicles and increases the amount of transforming growth factor (TGF) 2 receptors and other proteins on the plasma membrane...
  27. A Soft Topical Antiandrogenic Drug
    Wei Chu Xu; Fiscal Year: 2013
    ..expected to be active topically in androgen-dependent maladies of the skin and scalp, but to be hydrolyzed by plasma or tissue enzymes, thus terminating its systemic action...
  28. Alan Frazer; Fiscal Year: 2016
    ..the hormonal effects: (1) biotinylation studies on synaptosomal membranes will be carried out to examine the plasma membrane distribution of the SERT after hormone treatment;(2) using chronoamperometry, two approaches will be used,..
  29. CYNTHIA T MCEVOY; Fiscal Year: 2016
    ..and medication compliance, including urine cotinines levels, smoking questionnaires, pill counts and fasting plasma ascorbic acid levels...
  30. Alpha B crystallin as a novel therapeutic for SIRS and Sepsis
    Jonathan B Rothbard; Fiscal Year: 2011
    ..The last aim is to assay the levels of Cryab in murine plasma at various times after injection with LPS to determine whether the protein is a marker of the severity of the ..
  31. Charlotte Cunningham-Rundles; Fiscal Year: 2015
    ..normal somatic hyper-mutation and isotype switch, secrete immune globulins poorly, and fail to differentiate into plasma cells. The genetic causes of B cell dysfunction in CVID are largely unknown...
  32. FREDERIC TREMAINE BILLINGS; Fiscal Year: 2016
    ..The candidate has recently shown that intraoperative concentrations of plasma and urine markers of oxidant injury (F2-isoprostanes and isofurans) predict the development of postoperative AKI...
  33. Chronic Sleep Restriction Increases Immunity to Autoantigen: Role of the SNS
    Arnold E Postlethwaite; Fiscal Year: 2010
    ..increased serum levels of anti-CII antibodies, decreased CD8+ T cells, increased CD11b+ cells and changes in plasma levels of inflammatory cytokines and levels of SNS derived neuropeptide Y in spleen...
  34. Role for Na,K-ATPase in Nucleoplasmic Calcium Homeostasis
    Craig Gatto; Fiscal Year: 2010
    ..of these alternative physiological roles of the Na,K-ATPase mandate that the enzyme be delivered to specific sub-plasma membrane pools, which raises as yet unaddressed questions pertaining to Na,K-ATP maturation and membrane targeting...
  35. Vascular dysfunction and oxidative stress in primary amyloidosis
    RAYMOND QUEZON MIGRINO; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Primary systemic amyloidosis is a plasma cell dyscrasia that is often fatal especially if there is cardiac involvement...
  36. JAMES FRANCIS MCGINNIS; Fiscal Year: 2015
    ..Inductively coupled plasma mass spectrometry will quantitate cerium at the parts per billion levels...
  37. Arthur Brody; Fiscal Year: 2016
    ..throughout the study using participant reports, exhaled carbon monoxide (CO) levels, urine cotinine levels, and plasma nicotine, cotinine, and 3'-hydroxycotinine levels...
  38. Novel Peptide MPO Inhibitors for Treating Atherosclerosis
    Kirkwood A Pritchard; Fiscal Year: 2012
    ..These studies will be performed in C57BL/6J mice. Mice will be injected intraperitoneally (ip) and plasma levels of inhibitor will be determined by HPLC with respect to dose and time...
  39. A Thioredoxin Mimetic for Radiocontrast Nephropathy
    GARRY JOHN SOUTHAN; Fiscal Year: 2012
    ..At 24 hours plasma will be analyzed for renal function and biochemical, histologic, and immunohistochemical parameters of renal ..
  40. William M Nauseef; Fiscal Year: 2016
    ..In addition to PMN, a specific Group IIA phospholipase A2 (GpIIA-PLA2), which is present in plasma of infected animals, tears, and inflammatory fluid, exhibits potent activity to kill and degrade SA...
  41. Diane Lebesgue; Fiscal Year: 2014
    ..brain that can be observed within seconds to minutes, suggesting that estrogen binding molecules expressed at the plasma membrane participate in mediating cellular responses to estradiol...
  42. Osteocalcin and Metabolic Risk Factors. The Framingham Study
    Yi Hsiang Hsu; Fiscal Year: 2011
    ..including waist circumference, systolic blood pressure, fasting triglycerides, fasting HDL cholesterol, fasting plasma glucose, fasting plasma insulin, adiponectin concentrations and insulin resistance indices such as HOMA-IR and ..
  43. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..the membrane trafficking pathway occupied by the transferrin receptor, allowing them to rapidly cycle between the plasma membrane and early endosomes...
  44. Dengue Infected Endothelial Cells Enhance Immune Cell Activation
    Erich R Mackow; Fiscal Year: 2013
    ..Importantly, high levels of C3a are present in severely ill dengue patients and associated with severe disease, plasma leakage and shock...
  45. Cellular Immunity to Category A-C Viruses in Humans
    ROBERT WILLIAM FINBERG; Fiscal Year: 2013
    ..adaptive immune responses to virus infection and their effects on endothelial cell function, with a focus on the plasma leakage associated with viral hemorrhagic fevers caused by flaviviruses and hantaviruses...
  46. W Timothy Garvey; Fiscal Year: 2016
    ..The second clue was provided by metabolomic studies of plasma obtained from normal controls and insulin-resistant patients with T2DM...
  47. Twenty-second Annual Fanconi Anemia Research Fund Scientific Symposium
    GROVER CARLTON BAGBY; Fiscal Year: 2010
    ..abnormalities of FA genes and FA gene expression have been reported in patients with sporadic malignancies of plasma cells, leukemia, head and neck cancer, lung cancer, and ovarian cancer...
  48. Christie P Thomas; Fiscal Year: 2014
    ..We will also study the effect of plasma obtained from patients with toxemia and the effect of novel sFlt1 isoforms on GEC preproendothelin-1 expression ..
  49. PHILIPPE ANDRE GALLAY; Fiscal Year: 2014
    ..We demonstrated that C5A disrupts the HIV membrane, but preserves the integrity of the cellular plasma membrane...
  50. Donald B Jump; Fiscal Year: 2015
    ..MetS) or non-alcoholic fatty liver disease (NAFLD), however, have low a low ratio of 20:4w6 relative to 18:2w6 in plasma and liver when compared to healthy individuals. This outcome implicates a problem with PUFA metabolism...
  51. Molecular Mechanism of Protein Kinase Cbeta-Mediated Cholesterol Homeostasis
    Kamal D Mehta; Fiscal Year: 2010
    ..The dominant underlying factor for this disease includes elevated plasma low density lipoprotein (LDL)-cholesterol levels in association with hypertriglyceridemia, abdominal obesity, or ..
  52. THOMAS STERLING SCANLAN; Fiscal Year: 2014
    ..TR1 and TR2;instead, T1AM is a potent agonist of an orphan GPCR called TAAR1 and is an inhibitor of catecholamine plasma membrane vesicular packaging transporters...
  53. BRIAN JAMES MICKEY; Fiscal Year: 2015
    ..Specific Aim 1: NPY genotyping, plasma NPY, and behavioral characterization...
  54. 24th Annual Fanconi Anemia Research Fund Scientific Symposium
    GROVER CARLTON BAGBY; Fiscal Year: 2012
    ..Acquired abnormalities of FA genes and FA gene expression have been reported in patients with sporadic malignancies of plasma cells, leukemia, head and neck cancer, lung cancer, and ovarian cancer.
  55. Alzheimer's Disease Therapeutic
    Trevor P Castor; Fiscal Year: 2012
    ..Pharmacologic efficacy will also be measured, particularly in the brain and plasma compartments by total PKC activity, membrane/cytosolic PKC activity ratios, and phosphorylation of ERK 1/2 MAP ..
  56. Small Molecule targeting of NADPH oxidase in neutrophils
    Marie Dominique Filippi; Fiscal Year: 2010
    ..The NADPH superoxide complex consists of a plasma membrane-bound flavocytochrome b558 (cyt b) and a regulatory complex, termed p47phox-p67phox-p40phox, taht is ..
  57. Nitric Oxide in Hemodialysis AV Fistula Maturation
    Richard A Cohen; Fiscal Year: 2010
    ..Our focus on tissue and cellular gene expression rather than plasma concentrations of these NO mediators is based on our expectation that local expression is more directly relevant ..
  58. Predictors of Pregnancy Outcome in SLE and APS
    Jane E Salmon; Fiscal Year: 2012
    ..patients in 7 centers, obtained detailed medical and obstetrical information monthly, and serially collected plasma, serum, DNA, RNA, and urine...
  59. Estrogen Modulation of Auditory Processing
    Raphael Pinaud; Fiscal Year: 2012
    ..Auditory event-related potentials correlate with the plasma E2 levels in humans during the menstrual cycle and auditory processing is more efficient in younger and older ..
  60. Nitric Oxide Restoration &Heme Detoxification as Adjunctive Therapies for Cerebr
    LEONARDO JOSE DE MOURA CARVALHO; Fiscal Year: 2012
    ..On the other hand, plasma free heme has been incriminated in the genesis of CM...
  61. Sex Hormones in Postmenopausal Women in the Diabetes Prevention Program
    Catherine Kim; Fiscal Year: 2012
    ..We propose an ancillary study to the DPP, the Sex Hormones in Postmenopausal Women or SHIP study. Using plasma stored from the baseline data collection and 2 years after randomization, we propose to examine ESH (testosterone, ..
  62. Aldosterone Action In The Brain, Hypertension and Inflammation
    ELISE PEERY GOMEZ-SANCHEZ; Fiscal Year: 2012
    ..MR) is implicated in clinical hypertension and target organ damage even in patients who have normal or low plasma aldosterone levels...
  63. CFI Inactivation of Human Plasma
    TREVOR CASTOR; Fiscal Year: 2009
    ..concern in the health- care community -- the need for effective sterilization techniques for human blood plasma and plasma-derived products...
  64. KALLIKREIN KININ SYSTEM IN INFLAMMATORY BOWEL DISEASE
    Robert Colman; Fiscal Year: 2001
    ..Activation of the contact system (CS) in plasma produces plasma kallikrein which activates neutrophils, cleaves high molecular weight kininogen (HK) and releases ..
  65. Apoptosis and Trophoblast Fusion
    NEAL STEWART ROTE; Fiscal Year: 2010
    ..Syncytialization involves several processes, particularly cessation of cellular proliferation, redistribution of plasma membrane phospholipids to form a phosphatidylserine (PS)-rich exofacial surface, expression and insertion of ..
  66. Site-directed Chemotherapy for Breast Cancer using Novel Angiogenesis Inhibitor
    Shaker A Mousa; Fiscal Year: 2010
    ..The goal is to determine whether the anti-angiogenic and anti-proliferative actions of tetrac are initiated at the plasma membrane and not at the genomic level...
  67. EFFECT OF AGING ON INTERORGAN GLUTATHIONE HOMEOSTASIS
    Murad Ookhtens; Fiscal Year: 1999
    ..3.Identify-quantify key age-related changes in kinetics of turnover of plasma and extra-plasma thiol-disulfides. We will inject 35S-labeled GSH, cysteine (CYSH) or cystine (CYSS) i.v...
  68. Development of a High Efficiency Plasma Sterilizer
    BRIAN BROSDAHL; Fiscal Year: 2007
    ..applicant): This research supports the development and commercialization of a more effective low temperature gas plasma sterilizer (GPS)...
  69. Fatty Acids, Angiotensin and Oxidative Stress
    Brent Egan; Fiscal Year: 2004
    BACKGROUND: Obese hypertensives have elevated plasma non-esterified fatty acids (NEFAs) including oleic acid. Oleic acid induces a PKC-dependent increase of reactive oxygen species in vascular smooth muscle cells...
  70. Impact of Seasonal Variation in Hematocrit, NOx & ET-1 on Vascular Reactivity
    Deidre Wipke Tevis; Fiscal Year: 2009
    ..Preliminary data in healthy men suggest that hematocrit (Hct, a determinant of blood viscosity) peaks while plasma nitrite/nitrate (NOx, a stable metabolite of the vasodilator molecule, nitric oxide) troughs in late fall/early ..
  71. Dosimetry of Lipid-Derived Amino Acid Adducts by LC/MS
    Ian Blair; Fiscal Year: 2006
    ..this process is much lower than we observed with 4-oxo-2-nonenal The ability to quantify covalent modifications to plasma amino acids, plasma proteins, and arginine-rich histones will provide a dosimeter of exposure to lipid ..
  72. Molecular Regulation of Alpha-Synuclein Misfolding and Toxicity in Two Yeast Mode
    SHUBHIK DEBBURMAN; Fiscal Year: 2009
    ..Instead, a-synuclein localizes primarily to the yeast plasma membrane even when toxic...
  73. Plasma iNOS IVD Prognosticates Sepsis Prior to Organ Damage and Dysfunction
    Robert J Webber; Fiscal Year: 2010
    ..A clinical study which can be completed in 36 months or less will be conducted on our IVD test for plasma inducible nitric oxide synthase (iNOS) as an accurate and reliable plasma biomarker for detecting early the onset ..
  74. ASSEMBLY OF NASCENT PLASMA LIPOPROTEINS BY HEPATOCYTES
    Robert Hamilton; Fiscal Year: 1991
    ..Hepatocytes secrete most of the plasma lipoproteins, and these same cells are the principal site of their uptake and catabolism...
  75. BIOENERGETICS AND PROTON PUMPS IN MALARIA PARASITES
    Akhil Vaidya; Fiscal Year: 2007
    Transport across the plasma membrane is a critical feature of all cellular physiology. Many gatekeepers at the plasma membrane are assisted by ionic and electric gradients across the membrane...
  76. CORONARY ARTERY DISEASE AND POSTPRANDIAL LIPOPROTEINS
    Henry Ginsberg; Fiscal Year: 1992
    Epidemiologic evidence for an association between risk for developing CAD and fasting levels of plasma lipids, lipoproteins and apolipoproteins is derived from international cross-sectional data, prospective within-country population ..
  77. Identification of Causal Variants at Novel GWAS Loci Associated with Lipid Traits
    DANIEL JAMES RADER; Fiscal Year: 2010
    ..b>Plasma lipids including low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and ..
  78. A Microfluidic Device for Label-free HIV Virion Count at Point of Care Settings
    Xuanhong Cheng; Fiscal Year: 2010
    ..Measurement of the level of circulating virus in the blood plasma, or "viral load," is a critical parameter used to monitor disease progression and make treatment decisions...
  79. ASSEMBLY OF A NEURAL PLASMA MEMBRANE PROTEOLIPID
    Victor Sapirstein; Fiscal Year: 1990
    The focus of this grant is the assembly of the plasma membrane proteolipid protein (PMPLP) in the synaptic plasma membrane and in myelin...
  80. ROLE OF PLASMA MEMBRANE IN STIMULUS-SECRETION COUPLING
    Margarethe Hoenig; Fiscal Year: 1991
    The long-range objective of this research proposal is to determine the role of the plasma membrane of a glucose- responsive insulinoma in stimulus-secretion coupling by analyzing the mechanisms by which it controls calcium fluxes...
  81. REGULATION OF APOB SECRETION IN INBRED MOUSE STRAINS
    Li Shin Huang; Fiscal Year: 2009
    Elevated plasma levels of apolipoprotein B (apo B) and low density lipoprotein (LDL) are associated with a higher risk for atherosclerotic coronary heart disease (CHD), a leading cause of mortality in the industrialized world...