extracellular fluid

Summary

Summary: The fluid of the body that is outside of CELLS. It is the external environment for the cells.

Top Publications

  1. ncbi High interstitial fluid pressure - an obstacle in cancer therapy
    Carl Henrik Heldin
    Ludwig Institute for Cancer Research, Box 595, SE 751 24 Uppsala, Sweden
    Nat Rev Cancer 4:806-13. 2004
  2. ncbi Evidence for bulk flow of brain interstitial fluid: significance for physiology and pathology
    N Joan Abbott
    Centre for Neuroscience Research, GKT School of Biomedical Sciences, King s College London, Guy s Campus, London SE1 1UL, UK
    Neurochem Int 45:545-52. 2004
  3. ncbi Synaptic activity regulates interstitial fluid amyloid-beta levels in vivo
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 48:913-22. 2005
  4. ncbi Structure-brain exposure relationships in rat and human using a novel data set of unbound drug concentrations in brain interstitial and cerebrospinal fluids
    Markus Fridén
    Discovery DMPK, AstraZeneca R and D Molndal, SE 431 83 Molndal, Sweden
    J Med Chem 52:6233-43. 2009
  5. ncbi CSF as a surrogate for assessing CNS exposure: an industrial perspective
    Jiunn H Lin
    Department of Preclinical Drug Metabolism, Merck Research Laboratories, West Point, Pennsylvania, USA
    Curr Drug Metab 9:46-59. 2008
  6. ncbi Extracellular fluid volume and glomerular filtration rate in 1878 healthy potential renal transplant donors: effects of age, gender, obesity and scaling
    A Michael Peters
    Department of Nuclear Medicine, Royal Sussex County Hospital, Brighton, UK
    Nephrol Dial Transplant 27:1429-37. 2012
  7. ncbi Interstitial flow and its effects in soft tissues
    Melody A Swartz
    Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne, Switzerland
    Annu Rev Biomed Eng 9:229-56. 2007
  8. ncbi Interstitial fluid pressure as a prognostic factor in cervical cancer following radiation therapy
    Seung Gu Yeo
    Department of Radiation Oncology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea
    Clin Cancer Res 15:6201-7. 2009
  9. ncbi Long-term performance of interstial fluid pressure and hypoxia as prognostic factors in cervix cancer
    Anthony Fyles
    Department of Radiation Oncology, Princess Margaret Hospital Ontario Cancer Institute, Canada
    Radiother Oncol 80:132-7. 2006
  10. ncbi Comparison of brain extracellular fluid, brain tissue, cerebrospinal fluid, and serum concentrations of antiepileptic drugs measured intraoperatively in patients with intractable epilepsy
    Bernhard Rambeck
    Biochemical Laboratory of the Epilepsy Research Foundation, University of Munster, Germany
    Epilepsia 47:681-94. 2006

Detail Information

Publications303 found, 100 shown here

  1. ncbi High interstitial fluid pressure - an obstacle in cancer therapy
    Carl Henrik Heldin
    Ludwig Institute for Cancer Research, Box 595, SE 751 24 Uppsala, Sweden
    Nat Rev Cancer 4:806-13. 2004
    ..Lowering the tumour IFP with specific signal-transduction antagonists might be a useful approach to improving anticancer drug efficacy...
  2. ncbi Evidence for bulk flow of brain interstitial fluid: significance for physiology and pathology
    N Joan Abbott
    Centre for Neuroscience Research, GKT School of Biomedical Sciences, King s College London, Guy s Campus, London SE1 1UL, UK
    Neurochem Int 45:545-52. 2004
    ....
  3. ncbi Synaptic activity regulates interstitial fluid amyloid-beta levels in vivo
    John R Cirrito
    Department of Neurology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Neuron 48:913-22. 2005
    ..The findings also have important implications for treatment development...
  4. ncbi Structure-brain exposure relationships in rat and human using a novel data set of unbound drug concentrations in brain interstitial and cerebrospinal fluids
    Markus Fridén
    Discovery DMPK, AstraZeneca R and D Molndal, SE 431 83 Molndal, Sweden
    J Med Chem 52:6233-43. 2009
    ..It was, however, also observed that K(p,uu,CSF) overpredicts K(p,uu,brain) for highly effluxed drugs, indicating lower efflux capacity of the blood-cerebrospinal fluid barrier compared to the blood-brain barrier...
  5. ncbi CSF as a surrogate for assessing CNS exposure: an industrial perspective
    Jiunn H Lin
    Department of Preclinical Drug Metabolism, Merck Research Laboratories, West Point, Pennsylvania, USA
    Curr Drug Metab 9:46-59. 2008
    ..Depending on the physicochemical properties of drugs and the site/timing of CSF sampling, the unbound drug concentration at the biophase within the brain could differ significantly from the corresponding CSF drug concentration...
  6. ncbi Extracellular fluid volume and glomerular filtration rate in 1878 healthy potential renal transplant donors: effects of age, gender, obesity and scaling
    A Michael Peters
    Department of Nuclear Medicine, Royal Sussex County Hospital, Brighton, UK
    Nephrol Dial Transplant 27:1429-37. 2012
    Aim. The aim of this study was to investigate the influence of age, gender, obesity and scaling on glomerular filtration rate (GFR) and extracellular fluid volume (ECV) in healthy subjects.
  7. ncbi Interstitial flow and its effects in soft tissues
    Melody A Swartz
    Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne, Switzerland
    Annu Rev Biomed Eng 9:229-56. 2007
    ..Finally, we end with some discussion of interstitial transport issues in drug delivery, cell mechanobiology, and cell homing toward draining lymphatics...
  8. ncbi Interstitial fluid pressure as a prognostic factor in cervical cancer following radiation therapy
    Seung Gu Yeo
    Department of Radiation Oncology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea
    Clin Cancer Res 15:6201-7. 2009
    ..To investigate tumor interstitial fluid pressure as a prognostic factor for recurrence-free survival in patients with cervical cancer following radiation therapy...
  9. ncbi Long-term performance of interstial fluid pressure and hypoxia as prognostic factors in cervix cancer
    Anthony Fyles
    Department of Radiation Oncology, Princess Margaret Hospital Ontario Cancer Institute, Canada
    Radiother Oncol 80:132-7. 2006
    ..Using data from our prospective trial, we updated a cohort of patients treated with definitive radiation alone without chemotherapy, to assess the long-term prognostic impact of these microenvironmental features...
  10. ncbi Comparison of brain extracellular fluid, brain tissue, cerebrospinal fluid, and serum concentrations of antiepileptic drugs measured intraoperatively in patients with intractable epilepsy
    Bernhard Rambeck
    Biochemical Laboratory of the Epilepsy Research Foundation, University of Munster, Germany
    Epilepsia 47:681-94. 2006
    ..For comparison, AED levels were determined in brain tissue, subarachnoid CSF, and serum...
  11. ncbi Releasing signals, secretory pathways, and immune function of endogenous extracellular heat shock protein 72
    John D Johnson
    Department of Integrative Physiology, University of Colorado, Boulder, CO 80309 0354, USA
    J Leukoc Biol 79:425-34. 2006
    ..In addition, we present data that emphasize the importance of caution when conducting in vitro immunological tests of Hsp72 function...
  12. ncbi The ketogenic diet changes metabolite levels in hippocampal extracellular fluid
    Ramakrishna Samala
    Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA
    Neurochem Int 58:5-8. 2011
    ..We hypothesized that the KD leads to metabolic changes in the brain, which are reflected in the hippocampal extracellular fluid (hECF). CD1 mice were fed control or KD for 2-3 weeks since weaning...
  13. ncbi Effect of heterogeneous vasculature on interstitial transport within a solid tumor
    Jianbing Zhao
    Department of Mechanical and Aerospace Engineering, 212 MAE A, University of Florida, Gainesville, FL 32611, USA
    Microvasc Res 73:224-36. 2007
    ..In sensitivity analysis, predicted tracer uptake was enhanced by increasing vascular leakiness. Increasing the interstitial hydraulic conductivity relative to the surrounding tissue reduced the overall drug uptake...
  14. pmc Mechanically induced osteogenic differentiation--the role of RhoA, ROCKII and cytoskeletal dynamics
    Emily J Arnsdorf
    Bone and Joint R and D Center, VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA
    J Cell Sci 122:546-53. 2009
    ..Finally, we show RhoA activation and actin tension are negative regulators of both adipogenic and chondrogenic differentiation. However, an intact, dynamic actin cytoskeleton under tension is necessary for flow-induced gene expression...
  15. ncbi Perfusion CT: noninvasive surrogate marker for stratification of pancreatic cancer response to concurrent chemo- and radiation therapy
    Mi Suk Park
    Department of Diagnostic Radiology, Institute of Gastroenterology, Severance Hospital, Yonsei University College of Medicine, Seodaemun Ku, Shinchon Dong 134, Seoul 120 752, South Korea
    Radiology 250:110-7. 2009
    ....
  16. ncbi Regulation of tumor invasion by interstitial fluid flow
    Adrian C Shieh
    Institute of Bioengineering and Swiss Institute for Experimental Cancer Research, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Phys Biol 8:015012. 2011
    ..Thus, interstitial fluid flow demonstrates how important biophysical factors are in cancer, both by modulating cell behavior and coupling biophysical and biochemical signals...
  17. ncbi Interstitial fluid pressure in tumors: therapeutic barrier and biomarker of angiogenesis
    Sarah Jane Lunt
    Division of Applied Molecular Oncology, Princess Margaret Hospital Ontario Cancer Institute, University Health Network, Department of Medical Biophysics, University of Toronto, Toronto, Canada
    Future Oncol 4:793-802. 2008
    ..Pretreatment interstitial fluid pressure and the change in pressure during treatment may provide important predictive information that in the future will be used to optimize therapy in individual patients...
  18. ncbi Directional cell migration in an extracellular pH gradient: a model study with an engineered cell line and primary microvascular endothelial cells
    Ranjani K Paradise
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Exp Cell Res 319:487-97. 2013
    ..These results provide the first demonstration of preferential cell migration toward acid in a pH(e) gradient, with intriguing implications for directed cell migration in the tumor and wound healing environments...
  19. ncbi Rise in extracellular fluid volume during high sodium depends on BMI in healthy men
    Folkert W Visser
    Department of Nephrology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands
    Obesity (Silver Spring) 17:1684-8. 2009
    ..Therefore, we studied the relation between BMI and sodium-induced changes in extracellular fluid volume (ECFV; distribution volume of (125)I-iothalamate) in 78 healthy men, not selected for BMI...
  20. ncbi Cardioprotection by ecto-5'-nucleotidase (CD73) and A2B adenosine receptors
    Tobias Eckle
    Department of Anesthesiology and Intensive Care Medicine, Tubingen University Hospital, Hoppe Seyler Str 3, D 72076 Tubingen, Germany
    Circulation 115:1581-90. 2007
    ..Once generated, adenosine can activate cell-surface adenosine receptors (A1 AR, A2A AR, A2B AR, A3 AR). In the present study, we define the contribution of adenosine to cardioprotection by ischemic preconditioning...
  21. ncbi Icodextrin improves metabolic and fluid management in high and high-average transport diabetic patients
    Ramon Paniagua
    Unidad de Investigacion Medica en Enfermedades Nefrologicas, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Mexico City, Mexico
    Perit Dial Int 29:422-32. 2009
    ....
  22. ncbi Exploring the relationship between serotonin and brain-derived neurotrophic factor: analysis of BDNF protein and extraneuronal 5-HT in mice with reduced serotonin transporter or BDNF expression
    Matthew E Szapacs
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802 4615, USA
    J Neurosci Methods 140:81-92. 2004
    ....
  23. pmc A qualitative and quantitative proteomic study of human microdialysate and the cutaneous response to injury
    Carolyn Gill
    Centre for Proteomic Research, School of Biological Sciences, University of Southampton, UK
    AAPS J 13:309-17. 2011
    The extracellular fluid space is the site of intercellular communication and represents an important source of mediators that can shed light on the parenchymal environment...
  24. ncbi Noninvasive magnetic resonance imaging of transport and interstitial fluid pressure in ectopic human lung tumors
    Yaron Hassid
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
    Cancer Res 66:4159-66. 2006
    ..This work presents a noninvasive method for assessing the spatial distribution of tumor IFP and mapping barriers to drug delivery and transport...
  25. pmc Microfluidic enhancement of intramedullary pressure increases interstitial fluid flow and inhibits bone loss in hindlimb suspended mice
    Ronald Y Kwon
    La Jolla Bioengineering Institute, La Jolla, CA, USA
    J Bone Miner Res 25:1798-807. 2010
    ....
  26. ncbi Principles and applicability of CSF sampling for the assessment of CNS drug delivery and pharmacodynamics
    Danny D Shen
    Department of Pharmacy, Box 357630, University of Washington, Seattle, WA 98195, USA
    Adv Drug Deliv Rev 56:1825-57. 2004
    ..The applicability and limitations of CSF sampling for assessing the CNS availability and concentration-effect relationship of drug candidates with varying physicochemical properties during drug discovery and development is discussed...
  27. pmc Combined anti-angiogenic therapy targeting PDGF and VEGF receptors lowers the interstitial fluid pressure in a murine experimental carcinoma
    Agnieszka Kłosowska-Wardega
    Biomedical Center, Ludwig Institute for Cancer Research, Uppsala University, Uppsala, Sweden
    PLoS ONE 4:e8149. 2009
    ..Simultaneous targeting of VEGFR and PDGFR kinase activity may be a useful strategy to decrease tumor IFP, but the timing of the inhibitors should be carefully determined...
  28. ncbi The extracellular fluid-to-intracellular fluid volume ratio is associated with large-artery structure and function in hemodialysis patients
    Yao Ping Lin
    Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, ROC
    Am J Kidney Dis 42:990-9. 2003
    ..It is not clear how body fluid distribution affects large-artery structure and function in patients with ESRD...
  29. ncbi Associations between radiocurability and interstitial fluid pressure in human tumor xenografts without hypoxic tissue
    Einar K Rofstad
    Department of Radiation Biology, Oslo University Hospital, Oslo, Norway
    Clin Cancer Res 16:936-45. 2010
    ..The aim of this preclinical study was to investigate whether tumor radiocurability may be associated with IFP through hypoxia-independent mechanisms...
  30. pmc Radiocurability is associated with interstitial fluid pressure in human tumor xenografts
    Einar K Rofstad
    Group of Radiation Biology and Tumor Physiology, Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
    Neoplasia 11:1243-51. 2009
    ..Therefore, the observed association between tumor radiocurability and IFP was most likely an indirect consequence of a strong relationship between IFP and the fraction of acutely hypoxic cells...
  31. ncbi Dynamic contrast-enhanced magnetic resonance imaging of tumor interstitial fluid pressure
    Kristine Gulliksrud
    Department of Radiation Biology, Institute for Cancer Research, Oslo, Norway
    Radiother Oncol 91:107-13. 2009
    ..The potential usefulness of gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA)-based dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for assessing tumor IFP noninvasively was investigated in the present study...
  32. ncbi Inhibition of TGF-beta modulates macrophages and vessel maturation in parallel to a lowering of interstitial fluid pressure in experimental carcinoma
    Alexei V Salnikov
    Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden
    Lab Invest 85:512-21. 2005
    ..Our data emphasize a potential role of tumor macrophages in carcinoma physiology and identify these cells as potential stromal targets for treatment aimed to improve efficacy of chemotherapy...
  33. pmc Effect of vascular normalization by antiangiogenic therapy on interstitial hypertension, peritumor edema, and lymphatic metastasis: insights from a mathematical model
    Rakesh K Jain
    E L Steele Lab for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Cancer Res 67:2729-35. 2007
    ..Finally, decreased fluid convection into the peritumor tissue would decrease peritumor edema associated with brain tumors and ascites accumulation in the peritoneal or pleural cavity, a major complication with a number of malignancies...
  34. ncbi Linked deficiencies in extracellular PP(i) and osteopontin mediate pathologic calcification associated with defective PC-1 and ANK expression
    Kristen Johnson
    Veterans Affairs Medical Center, University of California San Diego, La Jolla, California 92161, USA
    J Bone Miner Res 18:994-1004. 2003
    ..Thus, linked regulatory effects on extracellular PP(i) and OPN expression mediate the ability of PC-1 and ANK to regulate calcification...
  35. ncbi Regulation of extracellular fluid volume and blood pressure by pendrin
    Juliette Hadchouel
    INSERM UMRS 970 Paris Cardiovascular Research Center PARCC, Paris, France
    Cell Physiol Biochem 28:505-12. 2011
    ....
  36. ncbi Exploratory translational modeling approach in drug development to predict human brain pharmacokinetics and pharmacologically relevant clinical doses
    W Kielbasa
    Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA
    Drug Metab Dispos 40:877-83. 2012
    ..this translational approach using rat brain microdialysis and PK modeling techniques to predict human brain extracellular fluid PK of atomoxetine and duloxetine...
  37. pmc Prion protein on astrocytes or in extracellular fluid impedes neurodegeneration induced by truncated prion protein
    Brent Race
    Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840 USA
    Exp Neurol 217:347-52. 2009
    ..results indicated that rescue from neurodegeneration induced by Delta32-134 PrP might involve interactions between neurons expressing truncated PrP and nearby astrocytes expressing WT PrP or extracellular fluid containing soluble WT PrP.
  38. ncbi Cardiovascular remodelling and extracellular fluid excess in early stages of chronic kidney disease
    Marie Essig
    Nephrologie, AP HP, Hopital Bichat Claude Bernard, Paris, France
    Nephrol Dial Transplant 23:239-48. 2008
    ..However, early stages of CKD and the mechanisms involved in these modifications have not been studied...
  39. pmc Interstitial flow induces MMP-1 expression and vascular SMC migration in collagen I gels via an ERK1/2-dependent and c-Jun-mediated mechanism
    Zhong Dong Shi
    City College of New York, City University of New York, Department of Biomedical Engineering, New York, NY 10031, USA
    Am J Physiol Heart Circ Physiol 298:H127-35. 2010
    ....
  40. pmc Mechanisms of proximal tubule sodium transport regulation that link extracellular fluid volume and blood pressure
    Alicia A McDonough
    Department of Cell and Neurobiology, University of Southern California, Los Angeles, California 90089 9142, USA
    Am J Physiol Regul Integr Comp Physiol 298:R851-61. 2010
    ..the past 25 years, the molecular mechanisms responsible for the interdependence of blood pressure (BP), extracellular fluid volume (ECFV), the renin-angiotensin system (RAS), and sympathetic nervous system (SNS) have begun to be ..
  41. ncbi Interstitial concentration of serotonin is increased in myalgic human trapezius muscle during rest, repetitive work and mental stress - an in vivo microdialysis study
    Bijar Ghafouri
    Rehabilitation Medicine, Department of Clinical and Experimental Medicine, Linköping University and Pain and Rehabilitation Centre, University Hospital, Linkoping, Sweden
    Scand J Clin Lab Invest 70:478-86. 2010
    ..Serotonin (5-HT) is involved in modulation of nociception and hyperalgesia. Our aim was to compare the interstitial 5-HT levels of the trapezius muscle in women with chronic trapezius myalgia and in pain-free controls...
  42. ncbi Unbound drug concentration in brain homogenate and cerebral spinal fluid at steady state as a surrogate for unbound concentration in brain interstitial fluid
    Xingrong Liu
    Roche Palo Alto, 3431 Hillview Avenue S3 2, Palo Alto, CA 94304, USA
    Drug Metab Dispos 37:787-93. 2009
    ....
  43. ncbi Elevated physiologic tumor pressure promotes proliferation and chemosensitivity in human osteosarcoma
    Saminathan S Nathan
    Orthopaedic Surgery Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, 1275A York Avenue, New York, NY 10021, USA
    Clin Cancer Res 11:2389-97. 2005
    ..This study investigates the effect of constitutively raised interstitial fluid pressure on osteosarcoma physiology and chemosensitivity...
  44. ncbi Collagenase increases the transcapillary pressure gradient and improves the uptake and distribution of monoclonal antibodies in human osteosarcoma xenografts
    Live Eikenes
    Department of Physics and Department of Circulation and Medical Imaging, The Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, Norway
    Cancer Res 64:4768-73. 2004
    ....
  45. ncbi Onset of abnormal blood and lymphatic vessel function and interstitial hypertension in early stages of carcinogenesis
    Jeroen Hagendoorn
    Edwin L Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, 100 Blossom Street, Boston, MA 02114, USA
    Cancer Res 66:3360-4. 2006
    ..In addition, the lymphatic vessels in hyperplastic/dysplastic lesions were partly compressed and nonfunctional. These novel insights may aid early detection and treatment strategies for cancer...
  46. pmc Cartilage interstitial fluid load support in unconfined compression
    Seonghun Park
    Department of Mechanical Engineering, Columbia University, SW Mudd, Mail Code 4703, 500 West, 120th Street, New York, NY, USA
    J Biomech 36:1785-96. 2003
    ....
  47. ncbi Extracellular alveolar proteasome: possible role in lung injury and repair
    Stephan Urs Sixt
    Klinik für Anästhesiologie und Intensivmedizin, Universitatsklinikum Essen, Hufelandstr 55, D 45122 Essen, Germany
    Proc Am Thorac Soc 7:91-6. 2010
    ..This article summarizes available information on the extracellular alveolar proteasome and its possible role in alveolar maintainance, lung injury, and repair...
  48. ncbi Differential proteomic analysis of renal cell carcinoma tissue interstitial fluid
    Pang Ning Teng
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    J Proteome Res 10:1333-42. 2011
    ....
  49. ncbi Proteomic characterization of the interstitial fluid perfusing the breast tumor microenvironment: a novel resource for biomarker and therapeutic target discovery
    Julio E Celis
    Danish Centre for Translational Breast Cancer Research, and Department of Proteomics in Cancer, Institute of Cancer Biology, The Danish Cancer Society, DK 2100 Copenhagen, Denmark
    Mol Cell Proteomics 3:327-44. 2004
    ....
  50. ncbi The ratio of extracellular fluid to total body water and technique survival in peritoneal dialysis patients
    Colin H Jones
    Renal Unit, York Hospital, York, United Kingdom
    Perit Dial Int 24:353-8. 2004
    ..We examined whether an increased extracellular fluid volume (VECF) as a proportion of the total body water (VTBW) predicted technique survival (TS) in a ..
  51. ncbi The human tumor microenvironment: invasive (needle) measurement of oxygen and interstitial fluid pressure
    Michael Milosevic
    Department of Radiation Oncology, Ontario Cancer Institute Princess Margaret Hospital, Toronto, Canada
    Semin Radiat Oncol 14:249-58. 2004
    ..The pathophysiology of elevated IFP is discussed, along with possible explanations for the strong influence on patient outcome and directions for future research...
  52. pmc Sustained and generalized extracellular fluid expansion following heat acclimation
    Mark J Patterson
    Department of Biological Science, University of Wollongong, NSW, Australia
    J Physiol 559:327-34. 2004
    ..0 +/- 1.8; day 8: 48.8 +/- 1.7; day 22: 48.8 +/- 2.0 ml kg(-1); P < 0.05). The extracellular fluid compartment (ECF) was equivalently expanded from control values on days 8 (279.6 +/- 14.2 versus 318...
  53. ncbi Microdialysis evaluation of the brain distribution of stavudine following intranasal and intravenous administration to rats
    Zheng Yang
    Department of Pharmaceutics, University of Minnesota, 308 Harvard St SE, Minneapolis, MN 55455, USA
    J Pharm Sci 94:1577-88. 2005
    ....
  54. pmc Ascorbate in pharmacologic concentrations selectively generates ascorbate radical and hydrogen peroxide in extracellular fluid in vivo
    Qi Chen
    Molecular and Clinical Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:8749-54. 2007
    ..After i.v. injection, ascorbate baseline concentrations of 50-100 microM in blood and extracellular fluid increased to peaks of >8 mM. After i.p. injection, peaks approached 3 mM in both fluids...
  55. ncbi Novel microdialysis method to assess neuropeptides and large molecules in free-moving mouse
    S Takeda
    Department of Clinical Gene Therapy, Osaka University, Yamadaoka, Osaka 565 0871, Japan
    Neuroscience 186:110-9. 2011
    Microdialysis is a powerful in vivo technique for the continuous sampling of small molecules within the extracellular fluid space. However, efforts to collect larger molecules have met with little success...
  56. ncbi Can extracellular fluid volume expansion in hemodialysis patients be safely reduced using the hemocontrol biofeedback algorithm? A randomized trial
    Gihad E Nesrallah
    Department of Nephrology, Humber River Regional Hospital, Toronto, Canada
    ASAIO J 54:270-4. 2008
    b>Extracellular fluid volume (ECFV) expansion in hemodialysis patients is associated with increased mortality. Attempts to remove excess fluid often result in intradialytic hypotension (IDH)...
  57. pmc Measuring interstitial fluid pressure with fiberoptic pressure transducers
    Ugur Ozerdem
    La Jolla Bioengineering Institute, 505 Coast Boulevard, South Suite 406, La Jolla, California 92037, USA
    Microvasc Res 77:226-9. 2009
    ....
  58. ncbi Central nervous system pharmacokinetics of the Mdr1 P-glycoprotein substrate CP-615,003: intersite differences and implications for human receptor occupancy projections from cerebrospinal fluid exposures
    Karthik Venkatakrishnan
    Department of Clinical Pharmacology, Pfizer Global Research and Development, Groton, Connecticut 06340, USA
    Drug Metab Dispos 35:1341-9. 2007
    ..Intracerebral microdialysis was used to characterize rat brain extracellular fluid (ECF) distribution...
  59. ncbi Extracellular gelsolin binds lipoteichoic acid and modulates cellular response to proinflammatory bacterial wall components
    Robert Bucki
    University of Pennsylvania, Institute for Medicine and Engineering, Philadelphia, PA 19104, USA
    J Immunol 181:4936-44. 2008
    ..These data suggest that extracellular gelsolin is involved in the host immune recognition of LTA or LPS following release of these molecules from the bacterial outer membrane during cell division or attack by drugs and immune components...
  60. pmc Tumor interstitial fluid pressure as an early-response marker for anticancer therapeutics
    Stephane Ferretti
    Oncology Research, Novartis Institute of Biomedical Research, 4002 Basel, Switzerland
    Neoplasia 11:874-81. 2009
    ..005) correlated with late changes in tumor volume. Thus, drug-induced decreases in tumor IFP are an early marker of response to therapy, which could aid clinical development...
  61. ncbi Oral L-glutamine increases GABA levels in striatal tissue and extracellular fluid
    Lei Wang
    Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    FASEB J 21:1227-32. 2007
    ..affects gamma-aminobutyric acid (GABA) levels in rat striatal tissue and GABA concentrations in striatal extracellular fluid (ECF)...
  62. ncbi pH is an intracellular effector controlling differentiation of oligodendrocyte precursors in culture via activation of the ERK1/2 pathway
    Frederic Bernard
    INSERM U 575, Physiopathologie du Système Nerveux, IFR des Neurosciences, Strasbourg, France
    J Neurosci Res 84:1392-401. 2006
    ..These results indicate that pH(i), acting through the ERK1/2 pathway, is a key determinant for oligodendrocyte differentiation. We also show that this pH(i) pathway is involved in the process of retinoic acid-induced OPC differentiation...
  63. ncbi Tumour interstitial fluid pressure may regulate angiogenic factors in osteosarcoma
    Saminathan S Nathan
    Musculoskeletal Oncology Service, Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Ann Acad Med Singapore 38:1041-7. 2009
    ..In this study, we hypothesized that constitutively raised IFP in osteosarcomas regulates angiogenesis...
  64. ncbi Controlled microscale diffusion gradients in quiescent extracellular fluid
    Darren Cherng Wen Tan
    Division of Bioengineering, National University of Singapore, Block E1, 05 22, 9 Engineering Drive 2, Singapore, 117576, Singapore
    Biomed Microdevices 12:523-32. 2010
    ..to generate temporally and spatially stable concentration gradients of multiple solutes in quiescent extracellular fluid. The fluorophore Alexa Fluor 488 and a fluorescent glucose analog are used as probes to illustrate the ..
  65. pmc Multidrug resistance-associated protein 1 decreases the concentrations of antiepileptic drugs in cortical extracellular fluid in amygdale kindling rats
    Ying Hui Chen
    Department of Neurology, Jinshan Hospital, Fudan University, Shanghai 201508, China
    Acta Pharmacol Sin 34:473-9. 2013
    ..To investigate whether multidrug resistance-associated protein 1 (MRP1) was responsible for drug resistence in refractory epilepsy in amygdale kindling rats...
  66. ncbi Increased interstitial concentrations of pyruvate and lactate in the trapezius muscle of patients with fibromyalgia: a microdialysis study
    Björn Gerdle
    Rehabilitation Medicine, Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden
    J Rehabil Med 42:679-87. 2010
    ..The aim of this study was to compare the interstitial concentrations of pyruvate, lactate and glutamate in the trapezius muscle between patients with fibromyalgia syndrome and healthy controls...
  67. ncbi Pharmacokinetics of gemcitabine in tumor and non-tumor extracellular fluid of brain: an in vivo assessment in rats employing intracerebral microdialysis
    Sandhya Kiran Apparaju
    College of Pharmacy, University of Cincinnati Medical Center, Cincinnati, OH 45267 0004, USA
    Cancer Chemother Pharmacol 61:223-9. 2008
    ..In this study we evaluated the gemcitabine brain extracellular fluid (ECF) in normal rats and in ECF obtained from tumor- and tumor-free regions of glioma-bearing rats, to ..
  68. pmc A novel function of insulin in rat dermis
    Torbjørn Nedrebø
    Division for Physiology, Department of Biomedicine, University of Bergen, Jonas Lies vei 91, N 5009 Bergen, Norway
    J Physiol 559:583-91. 2004
    ..Insulin can attenuate a lowering of Pif possibly via PI3K, and it has an anti-inflammatory effect by inhibiting production of pro-inflammatory cytokines...
  69. ncbi Extracellular fluid concentrations of cisplatin, carboplatin, and oxaliplatin in brain, muscle, and blood measured using microdialysis in nonhuman primates
    Shana Jacobs
    Pediatric Oncology Branch, NCI, National Institutes of Health, Bldg 10 CRC Rm 1 5750, 10 Center Drive, Bethesda, MD 20892, USA
    Cancer Chemother Pharmacol 65:817-24. 2010
    ..Microdialysis is a minimally invasive in vivo method for sampling small molecules in the blood and tissue extracellular fluid (ECF). The purpose of this study was to estimate the penetration of platinum analogs into the brain ECF.
  70. ncbi Assessment of tumor hypoxia and interstitial fluid pressure by gadomelitol-based dynamic contrast-enhanced magnetic resonance imaging
    Kristine Gulliksrud
    Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Norway
    Radiother Oncol 101:217-22. 2011
    ..The potential of gadomelitol-based dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in assessing the extent of hypoxia and the level of interstitial hypertension in tumors was investigated in this preclinical study...
  71. ncbi Assessment of extracellular fluid volume and fluid status in hemodialysis patients: current status and technical advances
    Yanna Dou
    Renal Research Institute, New York, New York 10128, USA
    Semin Dial 25:377-87. 2012
    The assessment of extracellular fluid volume (ECV) and fluid status is both important and challenging in hemodialysis patients. Extracellular fluid is distributed in two major sub-compartments: interstitial fluid and plasma...
  72. ncbi Simplified methods for assessment of renal function as the ratio of glomerular filtration rate to extracellular fluid volume
    Lars Jødal
    Department of Nuclear Medicine, Aalborg University Hospital, Aalborg, Denmark
    Nucl Med Commun 33:1243-53. 2012
    ..73 m(2), it has been suggested to scale GFR to extracellular fluid volume (ECV)...
  73. ncbi The peptide AF-16 decreases high interstitial fluid pressure in solid tumors
    Mohamed Al-Olama
    Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Goteborg, Sweden
    Acta Oncol 50:1098-104. 2011
    ..The high interstitial fluid pressure (IFP) in solid tumors restricts the access to nutrients, oxygen and drugs...
  74. ncbi Microenvironment-associated lymph node metastasis of human cervical carcinoma xenografts
    Christine Ellingsen
    Group of Radiation Biology and Tumor Physiology, Department of Radiation Biology, Institute for Cancer Research, Oslo University Hospital, Norway
    Acta Oncol 51:465-72. 2012
    ..The impact of the physiological microenvironment of the primary tumor on lymph node metastasis was investigated in this preclinical study...
  75. ncbi A pharmacokinetic study of diphenhydramine transport across the blood-brain barrier in adult sheep: potential involvement of a carrier-mediated mechanism
    Sam C S Au-Yeung
    Division of Pharmaceutics and Biopharmaceutics, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Drug Metab Dispos 34:955-60. 2006
    ..In six adult sheep, we characterized the central nervous system (CNS) pharmacokinetics of DPHM in brain extracellular fluid (ECF) and cerebrospinal fluid (CSF) using microdialysis in two experiments...
  76. ncbi Improving the recovery of S100B protein in cerebral microdialysis: implications for multimodal monitoring in neurocritical care
    R Afinowi
    Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N3BG, UK
    J Neurosci Methods 181:95-9. 2009
    ..The correct interpretation of body fluid biomarker levels depends on the degree of recovery, i.e. relative recovery and the accuracy of the analytical technique...
  77. ncbi Matrix metalloproteinase-9 concentration in the cerebral extracellular fluid of patients during the acute phase of aneurysmal subarachnoid hemorrhage
    Asita Sarrafzadeh
    Faculty of Medicine, University of Geneva, Switzerland
    Neurol Res 34:455-61. 2012
    ..The authors investigated MMP-9 concentration in brain extracellular fluid of aSAH patients and assessed whether this enzyme could have a predictive value for the risk of DCI.
  78. ncbi Contribution of intestine, bone, kidney, and dialysis to extracellular fluid calcium content
    David A Bushinsky
    Departments of Medicine and of Pharmacology and Physiology, University of Rochester School of Medicine, Rochester, New York, USA
    Clin J Am Soc Nephrol 5:S12-22. 2010
    ..In this article, we consider movement of Ca with respect to the extracellular fluid (ECF) and develop a mathematical formulation for Ca homeostasis with respect to the ECF that includes input ..
  79. pmc Amyloid-beta dynamics are regulated by orexin and the sleep-wake cycle
    Jae Eun Kang
    Department of Neurology, Washington University, St Louis, MO 63110, USA
    Science 326:1005-7. 2009
    ..Thus, the sleep-wake cycle and orexin may play a role in the pathogenesis of Alzheimer's disease...
  80. pmc Mild elevation of body temperature reduces tumor interstitial fluid pressure and hypoxia and enhances efficacy of radiotherapy in murine tumor models
    Arindam Sen
    Department of Immunology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    Cancer Res 71:3872-80. 2011
    ..These data suggest, for the first time, that environmental manipulation of normal vasomotor function is capable of achieving therapeutically beneficial changes in IFP and microvascular function in the tumor microenvironment...
  81. ncbi Chronic electrographic seizure reduces glutamine and elevates glutamate in the extracellular fluid of rat brain
    Keiko Kanamori
    Huntington Medical Research Institutes, 660 S Fair Oaks Ave, Pasadena, CA 91105, USA
    Brain Res 1371:180-91. 2011
    ..b>Extracellular fluid from the CA1-CA3 regions of the hippocampus was collected with a 2-mm microdialysis probe every 2 min for ..
  82. ncbi Brain extracellular fluid protein changes in acute stroke patients
    Loï Dayon
    Biomedical Proteomics Group, Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland
    J Proteome Res 10:1043-51. 2011
    ..Significant increases of 8- (p = 0.0002), 20- (p = 0.0001), and 11-fold (p = 0.0093) were found, respectively. This study highlights the value of ECF as an efficient source to further discover blood stroke markers...
  83. ncbi Proteomics of brain extracellular fluid (ECF) and cerebrospinal fluid (CSF)
    Martin H Maurer
    Department of Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany
    Mass Spectrom Rev 29:17-28. 2010
    ..the available literature on proteomic experiments that analyze human cerebrospinal fluid (CSF) and brain extracellular fluid (ECF), mostly obtained by cerebral microdialysis...
  84. pmc Autologous morphogen gradients by subtle interstitial flow and matrix interactions
    Mark E Fleury
    Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne EFPL, Lausanne, Switzerland
    Biophys J 91:113-21. 2006
    ....
  85. ncbi Discovery and neurochemical screening of peptides in brain extracellular fluid by chemical analysis of in vivo microdialysis samples
    William E Haskins
    Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA
    Anal Chem 76:5523-33. 2004
    Endogenous peptides from brain extracellular fluid of live rats were analyzed using capillary liquid chromatography (LC)-tandem mass spectrometry (MS2). A 4-mm-long microdialysis probe perfused at 0...
  86. ncbi Intracerebral microdialysis in neurosurgical intensive care patients utilising catheters with different molecular cut-off (20 and 100 kD)
    J Hillman
    Department of Neurosurgery, University Hospital, Linkoping, Sweden
    Acta Neurochir (Wien) 148:319-24; discussion 324. 2006
    ..To compare the properties of a new intracerebral micro-dialysis catheter with a high cut-off membrane (molecular cut-off 100 kDalton) with a standard catheter (CMA70, molecular cut-off 20 kDalton)...
  87. pmc Increased plasma colloid osmotic pressure facilitates the uptake of therapeutic macromolecules in a xenograft tumor model
    Matthias Hofmann
    Department of Dermatology and Venerology, Goethe University, D 60590 Frankfurt Main, Germany
    Neoplasia 11:812-22. 2009
    ..This model represents a novel approach to facilitate the delivery of therapeutics into tumor tissue, particularly monoclonal antibodies...
  88. pmc Effect of pazopanib on tumor microenvironment and liposome delivery
    Tina D Tailor
    Duke University School of Medicine, Durham, North Carolina, USA
    Mol Cancer Ther 9:1798-808. 2010
    ....
  89. ncbi "Whither flows the fluid in bone?" An osteocyte's perspective
    Melissa L Knothe Tate
    Department of Biomedical Engineering, ND 20, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    J Biomech 36:1409-24. 2003
    ....
  90. ncbi Paclitaxel decreases the interstitial fluid pressure and improves oxygenation in breast cancers in patients treated with neoadjuvant chemotherapy: clinical implications
    Alphonse G Taghian
    Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Clin Oncol 23:1951-61. 2005
    ..Our aim is to evaluate the IFP and pO(2) before and after neoadjuvant CT using sequential paclitaxel and doxorubicin in patients with breast cancer tumors of >/= 3 cm...
  91. pmc Amyloid-beta dynamics correlate with neurological status in the injured human brain
    David L Brody
    Department of Neurology, Washington University, St Louis, MO 63110, USA
    Science 321:1221-4. 2008
    ..Such dynamics fit well with the hypothesis that neuronal activity regulates extracellular Abeta concentration...
  92. pmc Synergy between interstitial flow and VEGF directs capillary morphogenesis in vitro through a gradient amplification mechanism
    Cara Lynn E Helm
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA
    Proc Natl Acad Sci U S A 102:15779-84. 2005
    ..This work thus demonstrates a general mechanism of morphogen gradient generation and amplification by small ubiquitous mechanical forces that are known to exist in vivo...
  93. pmc Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion
    Ines M Basalo
    Department of Mechanical Engineering, Columbia University, New York, NY 10027, USA
    J Biomech Eng 126:779-86. 2004
    ..0001). The drop in interstitial fluid load support following enzymatic treatment is believed to result from a decrease in the ratio of tensile to compressive moduli of the solid matrix...
  94. ncbi Neuro-inflammation induced in the hippocampus of 'binge drinking' rats may be mediated by elevated extracellular glutamate content
    Roberta J Ward
    Dipartimento di Farmacologia Preclinica e Clinica M Aiazzi Mancini, Universita degli Studi di Firenze, Firenze, Italia
    J Neurochem 111:1119-28. 2009
    ....
  95. ncbi The pharmacokinetic inter-relationship of tiagabine in blood, cerebrospinal fluid and brain extracellular fluid (frontal cortex and hippocampus)
    Xiaolan Wang
    Pharmacology and Therapeutics Unit, Department of Clinical and Experimental Epilepsy, Institute of Neurology, Queen Square, London WC1N 3BG, UK
    Seizure 13:574-81. 2004
    ..We therefore sought to investigate serum, cerebrospinal fluid (CSF) and frontal cortex and hippocampal extracellular fluid (ECF) kinetic inter-relationship of tiagabine in a freely moving rat model.
  96. ncbi Effects of estrogen and progesterone administration on extracellular fluid
    Nina S Stachenfeld
    Department of Epidemiology, Yale University School of Medicine, New Haven, CT 06519, USA
    J Appl Physiol (1985) 96:1011-8. 2004
    To determine the effect of estrogen and progesterone on plasma volume (PV) and extracellular fluid volume (ECFV), we suppressed endogenous estrogen and progesterone by using the gonadotropin-releasing hormone (GnRH) antagonist ganirelix ..
  97. ncbi Supraphysiological growth hormone: less fat, more extracellular fluid but uncertain effects on muscles in healthy, active young adults
    Christer Ehrnborg
    Research Centre for Endocrinology and Metabolism, Department of Internal Medicine, Sahlgrenska University Hospital, S 413 45 Goteborg, Sweden
    Clin Endocrinol (Oxf) 62:449-57. 2005
    ..To study the effects on body composition after 1 month's administration of supraphysiological doses of growth hormone (GH) in healthy, active young adults with normal GH-IGF-I axis...
  98. ncbi Mechanistic aspects of the Fenton reaction under conditions approximated to the extracellular fluid
    Wolfhardt Freinbichler
    Institute for Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria
    J Inorg Biochem 103:28-34. 2009
    The Fenton reaction was investigated, in a medium approximating to that of the extracellular fluid (ECF), by rapid-mixing stopped flow experiments and HPLC analysis using sodium terephthalate (TA(2-))...
  99. ncbi Fluid pressure gradients, arising from oscillations in intramedullary pressure, is correlated with the formation of bone and inhibition of intracortical porosity
    Yi Xian Qin
    Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, NY 11794 2580, USA
    J Biomech 36:1427-37. 2003
    ..While ImP increases uniformly in the marrow cavity, the distinct parameters of fluid flow vary substantially due to the geometry and ultrastructure of bone, which ultimately defines the spatial non-uniformity of the adaptive process...
  100. ncbi Suppression of glial glutamine release to the extracellular fluid studied in vivo by NMR and microdialysis in hyperammonemic rat brain
    Keiko Kanamori
    Magnetic Resonance Spectroscopy Laboratory, Huntington Medical Research Institutes, Pasadena, CA 91105, USA
    J Neurochem 94:74-85. 2005
    Release of glial glutamine (GLN) to the extracellular fluid (ECF), mainly mediated by the bidirectional system N transporter SN1, was studied in vivo in hyperammonemic rat brain, using (15)N-nuclear magnetic resonance (NMR) to monitor ..
  101. ncbi High-dose, short-term, anti-inflammatory treatment with dexamethasone reduces growth and augments the effects of 5-fluorouracil on dimethyl-alpha-benzanthracene-induced mammary tumors in rats
    L E B Stuhr
    Department of Biomedicine, University of Bergen, Bergen, Norway
    Scand J Clin Lab Invest 66:477-86. 2006
    ..To evaluate the effects of dexamethasone (DXM) alone or in combination with 5-fluorouracil (5-FU) on dimethyl-alpha-benzanthracene (DMBA)-induced mammary tumors in rats...

Research Grants63

  1. Hydrodynamic forces modulate renal tubular function
    Rajeev Rohatgi; Fiscal Year: 2012
    ..of salt and water transport in the collecting duct (CD) of the distal nephron, is regulated, in part, by extracellular fluid volume...
  2. VOLUME REGULATION IN NORMAL AND CATARACTOUS LENSES
    Richard T Mathias; Fiscal Year: 2013
    ..The circulation is critical for homeostasis in central fiber cells, which rely on the inwardly directed extracellular fluid flow to carry essential nutrients, amino acids and antioxidants into the lens...
  3. Cell Surface Plasminogen Activation and Hypertension
    Robert J Parmer; Fiscal Year: 2013
    ..in the reabsorption of filtered sodium, hence playing a crucial role in the regulation of sodium balance, extracellular fluid volume, and blood pressure...
  4. TRAFFIC REGULATORY PROTEINS AND ENAC
    Raymond A Frizzell; Fiscal Year: 2013
    ..channel number is determined by membrane trafficking events in response to key hormonal regulators of the extracellular fluid volume and blood pressure...
  5. Commercialization of iSONEP, a Humanized Monoclonal Antibody Against the Bioactiv
    ROGER ALLEN SABBADINI; Fiscal Year: 2010
    ..iSONEP acts as a molecular sponge to selectively absorb S1P from the extracellular fluid, lowering the effective concentration of S1P...
  6. Physiologic role of the gamma epithelial sodium channel subunit in the kidney
    Donald E Kohan; Fiscal Year: 2010
    ..where they serve as the final sites of Na reabsorption, thereby playing a key role in the regulation of extracellular fluid volume and blood pressure (BP)...
  7. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2012
    ..The concentration of Ca2+ in the extracellular fluid ([Ca2+]e) plays a key role in growth plate cartilage development...
  8. Phosphorylation-dependent regulation of epithelial sodium channel (ENaC) traffick
    Xiubin Liang; Fiscal Year: 2012
    ..ENaC regulation is critical for the overall control of sodium balance and extracellular fluid volume, and thereby of blood pressure...
  9. Mechanisms of rapid paravascular fluid transport in the brain
    Jeffrey J Iliff; Fiscal Year: 2012
    ..The brain does not have a conventional lymphatic system, so metabolic waste is cleared from brain extracellular fluid by its transport along the basal lamina, a sheath that surrounds brain capillaries...
  10. PROCESSING AND DEGRADATION OF PROTEOLIPID PROTEIN
    Robert P Skoff; Fiscal Year: 2013
    ..These metabolic changes cause (1) In vitro and in vivo, acidification of media and extracellular fluid (ECF), (2) activation of microglia, and (3) up-regulation of cytokines...
  11. SECRETION FROM INDIVIDUAL VESICLES
    ROBERT MARK WIGHTMAN; Fiscal Year: 2012
    ..view of the regulation of concentrations of chemical messengers from exocytosis to their removal from the extracellular fluid. Three specific proteins, each positioned at a central regulatory location, will be targets;for each ..
  12. Gamma-hydroxybutyrate: Toxicokinetics, Toxicodynamics and Treatment Strategies
    MARILYN EMILY MORRIS; Fiscal Year: 2013
    ..of MCT inhibitors, such as L-lactate, can increase the renal and total clearances of GHB, decrease brain extracellular fluid concentrations of GHB, and decrease sleep time in rats...
  13. The Mechanism of the Outer Blood-Retina Barrier Breakdown
    Zsolt Ablonczy; Fiscal Year: 2013
    ..Initial studies concluded that the principle source of the extracellular fluid was leakage from inner retina vessels...
  14. ENaC Regulation by Cell Surface Associated SGK1
    Alan C Pao; Fiscal Year: 2013
    ..applicant): Epithelial sodium channel (ENaC) activity in the collecting duct is essential for maintaining extracellular fluid volume and blood pressure...
  15. ELEMENTARY EVENTS OF INTRACELLULAR CALCIUM SIGNALING
    Ian Parker; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The entry of Ca2+ ions into the cytosol from the extracellular fluid and from endoplasmic reticulum (ER) stores is used as a signaling mechanism by virtually all cell types to regulate functions as ..
  16. Dynamics of pH Regulation in the Brain
    Mitchell Chesler; Fiscal Year: 2013
    ..by applicant): The electrical activity of neurons is associated with rapid changes in the pH of the brain extracellular fluid. Within milliseconds, a rise in extracelluar pH can be detected that is caused by activation of the plasma ..
  17. Calcium entry pathway in hypoxic pulmonary hypertension
    Jian Wang; Fiscal Year: 2010
    ..The mechanisms of AHPV and CHPV remain unknown. Evidence suggests that calcium influx from extracellular fluid into pulmonary artery smooth muscle cells (PASMCs) plays a role...
  18. mPGES-1/EP1 in volume regulation
    Tianxin Yang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Summary: The kidney plays a major role in the regulation of extracellular fluid volume (ECFV) and blood + + pressure (BP) via adjustment of urinary Na excretion in alignment with Na intake...
  19. MicroRNA as mediators of angiogenesis &ischemic myocardial repair
    Meifeng Xu; Fiscal Year: 2013
    ..in repair of ischemic myocardium;(ii) explore the impact of transfer of specific molecules from MSC to the extracellular fluid space via MVs, and directly into CM via gap junctions or other intercellular channels...
  20. OCT Blockade to Restore Sociability in 5-HT Transporter Knock-Out Mice
    Georgianna G Gould; Fiscal Year: 2012
    ..tightly controls the strength and duration of 5-HT neurotransmission by high affinity uptake of 5-HT from extracellular fluid in brain, but a role for organic cation transporter 3 (OCT3) in 5-HT uptake is emerging as an important ..
  21. Glucose and age-Related Memory Impairments
    Ken Morris; Fiscal Year: 2013
    ..Several findings suggest that fluctuations in glucose levels in the extracellular fluid of the brain may modulate memory processes by altering the release of the neurotransmitter acetylcholine...
  22. Conformational rearrangements underlying ASIC1a gating
    MARCELO DANIEL CARATTINO; Fiscal Year: 2013
    ..channels is constituted of proteins that are implicated in mechanosensation, pain sensation, regulation of extracellular fluid volume and airway surface liquid volume...
  23. Dynamics of pH Regulation in the Brain
    Mitchell Chesler; Fiscal Year: 2012
    ..This influx simultaneously acts to acidify the extracellular fluid. By contrast, depolarization of neurons slowly acidifies the cytosol, a response associated with entry of ..
  24. ELEMENTARY EVENTS OF INTRACELLULAR CALCIUM SIGNALING
    Ian Parker; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The entry of Ca2+ ions into the cytosol from the extracellular fluid and from endoplasmic reticulum (ER) stores is used as a signaling mechanism by virtually all cell types to regulate functions as ..
  25. Biochemical Studies of 14-kDa Phospholipases A2
    Michael H Gelb; Fiscal Year: 2013
    ..We will also test the possibility that the M-type sPLA2 receptor functions to clear sPLA2s from the extracellular fluid once they have been secreted from cells.
  26. Mechanisms and Regulation of Brain Iron Uptake
    Ian A Simpson; Fiscal Year: 2013
    ..cells store iron that can be released into the brain in response to external factors that are found in the extracellular fluid;thus for the first time demonstrating a regulatory system for movement of iron into the brain...
  27. Non-vesicular GABA release via GABA transporter reversal
    George B Richerson; Fiscal Year: 2011
    ..they have a particularly low threshold for reversal, and when they reverse they release GABA into the extracellular fluid. Even when they don't reverse they play an important role in regulation of the amount of tonic ..
  28. Epithelial Na Channels: Structure-Function Studies
    Thomas R Kleyman; Fiscal Year: 2010
    ..These channels have a key role in the regulation of urinary Na+ reabsorption, extracellular fluid volume homeostasis and blood pressure...
  29. Bio-ionic Neural Interfaces
    LUKE SATISH KUMAR THEOGARAJAN; Fiscal Year: 2010
    ..interface that circumvents these issues by using potassium ions that are sequestered from the extracellular fluid to chemically stimulate neural systems...
  30. Mechanisms of Exercise-Induced Protection and Rescue in Models of Dopamine Loss
    Michael J Zigmond; Fiscal Year: 2013
    ..1c) Levels of DA and metabolites in striatal tissue and extracellular fluid will be measured in MPTP animals treated with exercise as dictated by Aim 1a...
  31. Rehabilitation of Stress-Induced Insomnia
    Pingfu Feng; Fiscal Year: 2009
    ..2) polysomnographic recording, a classic technique for sleep evaluation, (3) microdialysis for extracellular fluid collection and (4) use radioimmunoassay to quantify the levels of CRH and orexins...
  32. ROLE OF CHLORIDE IN SODIUM-DEPENDENT HYPERTENSION
    Theodore Kurtz; Fiscal Year: 1990
    ..The changes in body weight, extracellular fluid volume, plasma volume, external balances of minerals and electrolytes, and plasma concentrations of renin, ..
  33. Integrated Tubular and Vascular Structure and Function in Renal Inner Medulla
    THOMAS LLOYD PANNABECKER; Fiscal Year: 2013
    ..PUBLIC HEALTH RELEVANCE: Extracellular fluid and solute homeostasis is important for maintaining normal cell function throughout the body...
  34. PHYSICOCHEMICAL INVESTIGATION OF TASTE
    Vijay Lyall; Fiscal Year: 2010
    Organisms have evolved mechanisms for controlling the acidity of their intracellular and extracellular fluid compartments to within narrow physiological limits...
  35. P-glycoprotein and Alzheimer's Disease
    Jashvant D Unadkat; Fiscal Year: 2010
    ..Recently, P-gp has been shown to transport amyloid-2, a protein which accumulates in the brain extracellular fluid in patients with Alzheimer's disease (AD)...
  36. Refining a structural model of ENaC
    Ossama B Kashlan; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The epithelial Na+ channel (ENaC) has a key role in the regulation of extracellular fluid volume and blood pressure. ENaC belongs to a family of ion channels that sense the external environment...
  37. Sodium Channel Biogenesis
    Thomas R Kleyman; Fiscal Year: 2013
    ..where they serve as the final site of renal Na+ reabsorption and have a key role in the regulation of extracellular fluid volume and blood pressure...
  38. Electroporation and transcutaneous extraction of drugs
    SATHYANARAYANA MURTHY; Fiscal Year: 2006
    unreadable] DESCRIPTION (provided by applicant): The time course of unbound drug in the the extracellular fluid (ECF) is critical in predicting the efficacy and toxicity of drugs such as antibiotics, anticancerous, antivirals etc...
  39. Signals Regulating Vestibular Endolymph Homeostasis
    Mark Henkemeyer; Fiscal Year: 2005
    ..Direct measurement of the extracellular fluid in the utricle reveals a highly significant decrease in endolymph potassium concentration and endolymphatic ..
  40. CALCIUM REGULATION IN ARTERIAL MYOCYTES
    William Goldman; Fiscal Year: 1993
    ..Ca2+ can enter the cytosol from the extracellular fluid through voltage-gated and/or receptor-operated channels...
  41. Insulin, Renal Sodium Transport and Blood Pressure
    Carolyn Ecelbarger; Fiscal Year: 2006
    ..Inappropriate retention of sodium in the distal portion of the tubule could result in expanded extracellular fluid volume and hypertension...
  42. REGULATION OF RENAL VASCULAR CELLS IN HYPERTENSION
    CRAIG GELBAND; Fiscal Year: 1999
    ..decrease, in renal blood flow lowers glomerular filtration rate triggering water retention and increases in extracellular fluid volume and venous return...
  43. ASTROCYTE MEDIATED ETHANOL NEUROTOXICITY
    Michael Aschner; Fiscal Year: 2000
    ..Withdrawal from chronic EtOH dependence is associated with increased extracellular fluid concentration of glutamate, contributing to hyperactivity of excitatory neurotransmission...
  44. The role of ketones in the anticonvulsant mechanism of the ketogenic diet
    Karin Borges; Fiscal Year: 2009
    ..To establish the composition of the brain extracellular fluid in KD-vo control diet fed mice, we will use in vivo microdialysis to sample and then quantify the ..
  45. BLOOD PRESSURE AND PROXIMAL TUBULE TRANSPORT
    FRANKLYN KNOX; Fiscal Year: 2000
    ..The efflux and influx of the extracellular fluid marker, lanthanum, between the renal proximal tubule lumen and interstitium will be used as a measure of ..
  46. MECHANISMS OF PRESSURE NATRIURESIS IN PROXIMAL TUBULES
    Kay Pong Yip; Fiscal Year: 2000
    ..filtration rate, which enables the kidney to use arterial pressure as an input signal to regulate the extracellular fluid volume. However, pressure natriuresis is set to higher blood pressure levels in all form of hypertension...
  47. PEPTIDE REGULATION OF THE CHOROID PLEXUS-CSF SYSTEM
    Conrad Johanson; Fiscal Year: 2002
    Choroid plexus (CP) has a great impact on the neuronal extracellular fluid environment...
  48. IRON INDUCED APOPTOSIS IN NEURODEGENERATIVE DISEASE
    Rae Nishi; Fiscal Year: 2002
    ..diferric recombinant transferrin in inducing apoptosis was 5nM, well within the levels found in embryonic extracellular fluid and blood (30-40 uM)...
  49. CELLULAR AND MOLECULAR BASIS OF BRAIN VOLUME REGULATION
    Kevin Strange; Fiscal Year: 1999
    Maintenance of the solute composition and volume of intra- and extracellular fluid compartments in the CNS is crucial for normal brain functioning...
  50. Etiology of Age-Related Defects in Urine Concentration
    Ying Tian; Fiscal Year: 2001
    ..These include: 1) decreased extracellular fluid water (dehydration, Aim 1); 2) increased extracellular fluid water (water loading, Aim 2); and 3) altered ..
  51. SURFACTANT PROTEINS A AND D AND MYCOBACTERIA
    DENNIS VOELKER; Fiscal Year: 2004
    ..Surfactant protein A (SP-A) and surfactant protein D (SP-D) are two abundant proteins present in the extracellular fluid of the alveolus that directly interact with mycobacteria and the target cell for replication, the alveolar ..
  52. APPROACHES TO NATRIURETIC AND ANTIHYPERTENSIVE AGENTS
    William Wechter; Fiscal Year: 2001
    DESCRIPTION: The goal of our program is the mechanistic understanding of the pharmacologic control of extracellular fluid volume in humans...
  53. EPENDYMIN, AN EXTRACELLULAR PROTEIN, & NEURAL FUNCTION
    VICTOR SHASHOUA; Fiscal Year: 1990
    The brain extracellular fluid (ECF) has an important role in maintaining the brain microenvironment for mental health and function...
  54. REGULATION OF RENAL VASCULAR CELLS IN HYPERTENSION
    CRAIG GELBAND; Fiscal Year: 2001
    ..decrease in renal blood flow lowers glomerular filtration rate, triggering water retention and increases in extracellular fluid volume, cardiac output and arterial pressure...
  55. Analysis of endocytosis in Caenorhabditis elegans
    Hanna Fares; Fiscal Year: 2006
    Endocytosis, the internalization and processing of extracellular fluid and macromolecules, is a basic cellular process that is used by the cells of most organisms for nutrient uptake and for the recycling of membrane...
  56. Internal environment of the developing brain
    Norman Saunders; Fiscal Year: 2006
    ..The composition of the fluids (brain extracellular fluid, ECF and cerebrospinal fluid, CSF) that form the internal environment of brain is controlled by mechanisms ..
  57. Optical Measurement of Fast Drug Kinetics in Tumor
    Irving Bigio; Fiscal Year: 2006
    ..Further OP measurements over the subsequent tens of minutes will permit assessment of the leakage into the extracellular fluid space and evaluation of microvessel permeability (hence, assessing capillary integrity) in regions of ..
  58. PINOCYTIC REGULATION IN NORMAL AND TRANSFORMED CELLS
    Joel Swanson; Fiscal Year: 1990
    Nearly all eukaryotic cells exhibit fluid phase pinocytosis, the cellular internalization of extracellular fluid in plasma membrane derived vesicles...
  59. Neural Mechanisms in Congestive Heart Failure
    GLENN TONEY; Fiscal Year: 2007
    ..Second, chronic extracellular fluid accumulation reduces cardiopulmonary reflex function and thus inhibitory restraint of SNA...
  60. ANALYSIS OF DENDRIMER BASED MRI CONTRAST AGENTS
    Erik Wiener; Fiscal Year: 1999
    ..The third is to develop extracellular fluid space agents with higher relaxivities and slightly prolonged half-lives for improving dynamic contrast ..
  61. MECHANISM OF TREATMENT COMPLICATIONS IN PARKINSONS
    Robert Schwartzman; Fiscal Year: 1991
    ..This results in high peak brain extracellular fluid levels of L-dopa, which is disproportionately metabolized to 3-0-methyldopa instead of dopamine...
  62. Nucleotides/fluid flow effects on osteoblastic cells
    Jun You; Fiscal Year: 2004
    It is well known that extracellular fluid flow-induced by mechanical loading increases osteoblastic cell metabolism, differentiation, extracellular matrix protein production and gene expression...
  63. MECHANISM OF CALCIUM MINERALIZATION
    RANDALL HUDSON; Fiscal Year: 1992
    Biomineralization is the physiological process by which soluble constituents of the extracellular fluid, principally calcium, phosphate and carbonate, are brought together under conditions which favor the formation of a crystalline ..