bronchoalveolar lavage fluid

Summary

Summary: Fluid obtained by washout of the alveolar compartment of the lung. It is used to assess biochemical and inflammatory changes in and effects of therapy on the interstitial lung tissue.

Top Publications

  1. ncbi Interleukin-13: central mediator of allergic asthma
    M Wills-Karp
    Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA
    Science 282:2258-61. 1998
  2. pmc Analysis of the lung microbiome in the "healthy" smoker and in COPD
    John R Erb-Downward
    University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS ONE 6:e16384. 2011
  3. pmc Topographical continuity of bacterial populations in the healthy human respiratory tract
    Emily S Charlson
    University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Am J Respir Crit Care Med 184:957-63. 2011
  4. pmc Disordered microbial communities in asthmatic airways
    Markus Hilty
    National Heart and Lung Institute, Imperial College London, London, England
    PLoS ONE 5:e8578. 2010
  5. pmc Requirement for IL-13 independently of IL-4 in experimental asthma
    G Grunig
    Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, CA 94143, USA
    Science 282:2261-3. 1998
  6. ncbi Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes
    Dale W Porter
    National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Toxicology 269:136-47. 2010
  7. pmc Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis
    J Kirk Harris
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 104:20529-33. 2007
  8. ncbi Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice
    Anna A Shvedova
    Health Effects Laboratory Div, NIOSH, Morgantown, WV, USA
    Am J Physiol Lung Cell Mol Physiol 289:L698-708. 2005
  9. ncbi Respiratory toxicity of multi-wall carbon nanotubes
    Julie Muller
    Industrial Toxicology and Occupational Medicine Unit, Universite Catholique de Louvain, Clos Chapelle aux Champs, 30 54 1200 Brussels, Belgium
    Toxicol Appl Pharmacol 207:221-31. 2005
  10. ncbi Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital and Lung Research Laboratory, Hanson Institute, Adelaide, South Australia
    Am J Respir Cell Mol Biol 37:748-55. 2007

Detail Information

Publications366 found, 100 shown here

  1. ncbi Interleukin-13: central mediator of allergic asthma
    M Wills-Karp
    Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205, USA
    Science 282:2258-61. 1998
    ..Thus, IL-13 is critical to allergen-induced asthma but operates through mechanisms other than those that are classically implicated in allergic responses...
  2. pmc Analysis of the lung microbiome in the "healthy" smoker and in COPD
    John R Erb-Downward
    University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS ONE 6:e16384. 2011
    ..These studies are further demonstration of the pulmonary microbiome and highlight global and micro-anatomic changes in these bacterial communities in severe COPD patients...
  3. pmc Topographical continuity of bacterial populations in the healthy human respiratory tract
    Emily S Charlson
    University of Pennsylvania School of Medicine, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Am J Respir Crit Care Med 184:957-63. 2011
    ..However, studies of the lung are challenging due to difficulties in working with low biomass samples...
  4. pmc Disordered microbial communities in asthmatic airways
    Markus Hilty
    National Heart and Lung Institute, Imperial College London, London, England
    PLoS ONE 5:e8578. 2010
    ..We compared the airway microbiota at three levels in adult patients with asthma, the related condition of COPD, and controls. We also studied bronchial lavage from asthmatic children and controls...
  5. pmc Requirement for IL-13 independently of IL-4 in experimental asthma
    G Grunig
    Department of Medicine, San Francisco General Hospital, University of California San Francisco, San Francisco, CA 94143, USA
    Science 282:2261-3. 1998
    ..This pathway may underlie the genetic associations of asthma with both the human 5q31 locus and the IL-4 receptor...
  6. ncbi Mouse pulmonary dose- and time course-responses induced by exposure to multi-walled carbon nanotubes
    Dale W Porter
    National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
    Toxicology 269:136-47. 2010
    ..Furthermore, the observation that MWCNT reach the pleura after aspiration exposure indicates that more extensive investigations are needed to fully assess if pleural penetration results in any adverse health outcomes...
  7. pmc Molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis
    J Kirk Harris
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Proc Natl Acad Sci U S A 104:20529-33. 2007
    Culture of bronchoalveolar lavage fluid (BALF) is the gold standard for detection of pathogens in the lower airways in cystic fibrosis (CF)...
  8. ncbi Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice
    Anna A Shvedova
    Health Effects Laboratory Div, NIOSH, Morgantown, WV, USA
    Am J Physiol Lung Cell Mol Physiol 289:L698-708. 2005
    ..Equal doses of ultrafine carbon black particles or fine crystalline silica (SiO2) did not induce granulomas or alveolar wall thickening and caused a significantly weaker pulmonary inflammation and damage...
  9. ncbi Respiratory toxicity of multi-wall carbon nanotubes
    Julie Muller
    Industrial Toxicology and Occupational Medicine Unit, Universite Catholique de Louvain, Clos Chapelle aux Champs, 30 54 1200 Brussels, Belgium
    Toxicol Appl Pharmacol 207:221-31. 2005
    ..These results suggest that carbon nanotubes are potentially toxic to humans and that strict industrial hygiene measures should to be taken to limit exposure during their manipulation...
  10. ncbi Smoking alters alveolar macrophage recognition and phagocytic ability: implications in chronic obstructive pulmonary disease
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital and Lung Research Laboratory, Hanson Institute, Adelaide, South Australia
    Am J Respir Cell Mol Biol 37:748-55. 2007
    ..Our data indicates that a smoking-related reduction in AM phagocytic ability and expression of several important recognition molecules may be at least partially normalized in those subjects with COPD who have ceased smoking...
  11. pmc Inflammasome-regulated cytokines are critical mediators of acute lung injury
    Tamas Dolinay
    Division of Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Am J Respir Crit Care Med 185:1225-34. 2012
    ..The inflammasome/caspase-1 pathway regulates the maturation and secretion of proinflammatory cytokines (e.g., IL-18). IL-18 is associated with injury in animal models of systemic inflammation...
  12. ncbi Proinflammatory exosomes in bronchoalveolar lavage fluid of patients with sarcoidosis
    Khaleda R Qazi
    Department of Medicine, Clinical Allergy Research Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden
    Thorax 65:1016-24. 2010
    ..Exosomes are nano-sized immunomodulatory vesicles of endosomal origin released from a diverse range of cells and are also found in physiological fluids including bronchoalveolar lavage fluid (BALF) from healthy individuals.
  13. pmc Oxidative stress and airway inflammation in severe exacerbations of COPD
    E M Drost
    ELEGI Laboratory, MRC Centre for Inflammation Research, University of Edinburgh, Medical School, Teviot Place, Edinburgh EH8 9AG, UK
    Thorax 60:293-300. 2005
    ..Two sites within the lungs were compared: the large airways (in sputum) and the peripheral airways (by bronchoalveolar lavage (BAL))...
  14. ncbi Increased airway epithelial and T-cell apoptosis in COPD remains despite smoking cessation
    S Hodge
    Dept of Thoracic Medicine, Royal Adelaide Hospital and Hanson Institute, Adelaide, South Australia 5001, Australia
    Eur Respir J 25:447-54. 2005
    ....
  15. ncbi Soluble triggering receptor expressed on myeloid cells and the diagnosis of pneumonia
    Sebastien Gibot
    Service de Reanimation Medicale, Hopital Central, Nancy, France
    N Engl J Med 350:451-8. 2004
    ..The presence of soluble TREM-1 (sTREM-1) in bronchoalveolar-lavage fluid from patients receiving mechanical ventilation may be an indicator of pneumonia...
  16. pmc Metal oxide nanoparticles induce unique inflammatory footprints in the lung: important implications for nanoparticle testing
    Wan Seob Cho
    ELEGI The Edinburgh Lung and the Environment Group Initiative, Centre for Inflammation Research, University of Edinburgh, Edinburgh, United Kingdom
    Environ Health Perspect 118:1699-706. 2010
    ..Metal oxide nanoparticles (NPs) have been widely used in industry, cosmetics, and biomedicine...
  17. pmc A persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations
    Yvonne J Huang
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of California San Francisco, San Francisco, California 94143 0538, USA
    OMICS 14:9-59. 2010
    ....
  18. pmc Smoking-dependent reprogramming of alveolar macrophage polarization: implication for pathogenesis of chronic obstructive pulmonary disease
    Renat Shaykhiev
    Department of Genetic Medicine, Weill Cornel Medical College, New York, NY 10065, USA
    J Immunol 183:2867-83. 2009
    ..In summary, the data provide transcriptome-based evidence that AM likely contribute to COPD pathogenesis in a noninflammatory manner due to their smoking-induced reprogramming toward M1-deactivated, partially M2-polarized macrophages...
  19. ncbi Role of p38 mitogen-activated protein kinase in a murine model of pulmonary inflammation
    J A Nick
    Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Immunol 164:2151-9. 2000
    ....
  20. pmc Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus disease
    Hye Youn Cho
    Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, 27709, USA
    Am J Respir Crit Care Med 179:138-50. 2009
    ..The transcription factor Nrf2 protects lungs from oxidative injury and inflammation via antioxidant response element (ARE)-mediated gene induction...
  21. ncbi Subchronic 13-week inhalation exposure of rats to multiwalled carbon nanotubes: toxic effects are determined by density of agglomerate structures, not fibrillar structures
    Jürgen Pauluhn
    Department of Inhalation Toxicology, Institute of Toxicology, Bayer Schering Pharma, Building Number 514, 42096 Wuppertal, Germany
    Toxicol Sci 113:226-42. 2010
    ..Thus, the hypothesis of study is verified, namely, common denominators between carbon black and MWCNT do exist...
  22. ncbi A vicious circle of alveolar macrophages and fibroblasts perpetuates pulmonary fibrosis via CCL18
    Antje Prasse
    Department of Pneumology, University Hospital, Killianstr 5, 79106 Freiburg, Germany
    Am J Respir Crit Care Med 173:781-92. 2006
    ..Recently, models of macrophage activation have been revised. Macrophages stimulated with Th2 cytokines have been classified as alternatively activated...
  23. pmc Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense
    P Ye
    Louisiana State University Health Sciences Gene Therapy Program, Section of Pulmonary Critical Care Medicine, New Orleans, LA 70112, USA
    J Exp Med 194:519-27. 2001
    ..pneumoniae infection. These data support impaired IL-17R signaling as a potential mechanism by which deficiency of CD4 lymphocytes predisposes to bacterial pneumonia...
  24. ncbi Cytokine balance in the lungs of patients with acute respiratory distress syndrome
    W Y Park
    Section of Pulmonary/Critical Care Medicine, Harborview Medical Center, Medical Research Service of the VA Puget Sound Health Care System, Seattle, Washington, USA
    Am J Respir Crit Care Med 164:1896-903. 2001
    ..inflammatory response in the lungs, with accumulation of both pro- and antiinflammatory cytokines in bronchoalveolar lavage fluid (BALF)...
  25. ncbi Airway dendritic cell phenotypes in inflammatory diseases of the human lung
    M Lommatzsch
    Abteilung fur Pneumologie, Klinik und Poliklinik für Innere Medizin, Universitat Rostock, Ernst Heydemann Str 6, 18057, Rostock, Germany
    Eur Respir J 30:878-86. 2007
    ..Plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were analysed using four-colour flow cytometry in bronchoalveolar lavage fluid (BALF) from nonsmoking controls and patients with sarcoidosis, idiopathic pulmonary fibrosis (IPF) and ..
  26. pmc Transient expression of IL-1beta induces acute lung injury and chronic repair leading to pulmonary fibrosis
    M Kolb
    Department of Pathology and Molecular Medicine and Centre for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada
    J Clin Invest 107:1529-36. 2001
    ..Although PDGF returned to baseline in the third week, TGF-beta1 showed increased concentrations in bronchoalveolar lavage fluid for up to 60 days...
  27. ncbi Novel murine model of chronic granulomatous lung inflammation elicited by carbon nanotubes
    Isham Huizar
    Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Brody School of Medicine, East Carolina University, 3E 149 Brody Medical Sciences Building, Greenville, NC 27834, USA
    Am J Respir Cell Mol Biol 45:858-66. 2011
    ..The establishment of such a model provides an important platform for mechanistic studies on the persistence of granuloma...
  28. ncbi Differential protease, innate immunity, and NF-kappaB induction profiles during lung inflammation induced by subchronic cigarette smoke exposure in mice
    R Vlahos
    Department of Medicine, University of Melbourne, Royal Melbourne Hospital, Parkville, VIC 3050, Australia
    Am J Physiol Lung Cell Mol Physiol 290:L931-45. 2006
    ..The response pattern we observed suggests that subchronic cigarette smoke exposure may be useful to understand pathogenic mechanisms triggered by cigarette smoke in the lungs including inflammation and alteration of host defense...
  29. ncbi Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles
    Christie M Sayes
    DuPont Haskell Laboratory for Health and Environmental Sciences, Newark, Delaware 19714, USA
    Toxicol Sci 97:163-80. 2007
    ..It seems clear that in vitro cellular systems will need to be further developed, standardized, and validated (relative to in vivo effects) in order to provide useful screening data on the relative toxicity of inhaled particle types...
  30. pmc Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers
    Murali Shyamsundar
    The Queen s University of Belfast, Grosvenor Road, Belfast, UK
    Am J Respir Crit Care Med 179:1107-14. 2009
    ..As simvastatin modulates a number of the underlying processes described in acute lung injury (ALI), it may be a potential therapeutic option...
  31. pmc Receptor for advanced glycation end-products is a marker of type I cell injury in acute lung injury
    Tokujiro Uchida
    Department of Anesthesiology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Am J Respir Crit Care Med 173:1008-15. 2006
    ..Receptor for advanced glycation end-products (RAGE) is one of the alveolar type I cell-associated proteins in the lung...
  32. ncbi Pulmonary and systemic immune response to inhaled multiwalled carbon nanotubes
    Leah A Mitchell
    College of Pharmacy, University of New Mexico, Albuquerque, New Mexico 87131 0001, USA
    Toxicol Sci 100:203-14. 2007
    ..No changes in gene expression were observed in lung; however, interleukin-10 (IL-10) and NAD(P)H oxidoreductase 1 mRNA levels were increased in spleen...
  33. ncbi Regulation of lung injury and repair by Toll-like receptors and hyaluronan
    Dianhua Jiang
    Department of Medicine, Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nat Med 11:1173-9. 2005
    ..Hyaluronan-TLR2 and hyaluronan-TLR4 interactions provide signals that initiate inflammatory responses, maintain epithelial cell integrity and promote recovery from acute lung injury...
  34. ncbi Ventilator-associated pneumonia
    Jean Chastre
    Service de Reanimation Medicale, Groupe Hospitalier Pitie Salpetriere, France
    Am J Respir Crit Care Med 165:867-903. 2002
    ..Further trials will be needed to clarify the optimal duration of treatment and the circumstances in which monotherapy can be safely used...
  35. ncbi Curcumin attenuates allergic airway inflammation and hyper-responsiveness in mice through NF-κB inhibition
    Se Woong Oh
    Central Research Institute, Choongwae Pharma Corp, Hwaseong, Republic of Korea
    J Ethnopharmacol 136:414-21. 2011
    ..has been used for centuries as an anti-inflammatory remedy including asthma. Curcumin has been reported to exert an anti-inflammatory effect, in part, through inhibition of the NF-κB pathway...
  36. pmc Mechanisms for how inhaled multiwalled carbon nanotubes suppress systemic immune function in mice
    L A Mitchell
    Lovelace Respiratory Research Institute, Albuquerque, New Mexico, USA
    Nat Nanotechnol 4:451-6. 2009
    ..Our findings suggest that signals from the lung can activate signals in the spleen to suppress the immune function of exposed mice...
  37. pmc De novo assembly of the Pneumocystis jirovecii genome from a single bronchoalveolar lavage fluid specimen from a patient
    Ousmane H Cissé
    Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland
    MBio 4:e00428-12. 2012
    ..We report here the genome of P. jirovecii that was obtained from a single bronchoalveolar lavage fluid specimen from a patient...
  38. pmc Repertoire of intensive care unit pneumonia microbiota
    Sabri Bousbia
    URMITE, Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Faculte de Medecine, CNRS IRD UMR 6236, Marseille, France
    PLoS ONE 7:e32486. 2012
    ..Differences in the microbiota of different forms of pneumonia were documented...
  39. pmc Interleukin 5 deficiency abolishes eosinophilia, airways hyperreactivity, and lung damage in a mouse asthma model
    P S Foster
    Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australia
    J Exp Med 183:195-201. 1996
    ..These results indicate that IL-5 and eosinophils are central mediators in the pathogenesis of allergic lung disease...
  40. ncbi Comparative pulmonary toxicity study of nano-TiO(2) particles of different sizes and agglomerations in rats: different short- and long-term post-instillation results
    Norihiro Kobayashi
    Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8569, Japan
    Toxicology 264:110-8. 2009
    ..Consequently, our findings indicate that both short- and long-term effects should be evaluated when assessing the toxicity of nanoparticles based on the results of intratracheal instillation studies...
  41. ncbi Cigarette smoke-mediated inflammatory and oxidative responses are strain-dependent in mice
    Hongwei Yao
    Lung Biology and Disease Program, Deptartment of Environmental Medicine, University of Rochester Medical Center, 601 Elmwood Ave, Box 850, Rochester, New York 14642, USA
    Am J Physiol Lung Cell Mol Physiol 294:L1174-86. 2008
    ..Identification of sensitive and resistant mouse strains could be useful for studying the molecular mechanisms of effects of CS on inflammation and pharmacological interventional studies in CS-exposure mouse models...
  42. pmc Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand
    Susanne Herold
    University of Giessen Lung Center, Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine and Infectious Diseases, Justus Liebig University, Giessen, Germany
    J Exp Med 205:3065-77. 2008
    ..Epithelial cell apoptosis induced by TRAIL-expressing macrophages is identified as a major underlying mechanism...
  43. ncbi Transforming growth factor-beta(1) in bronchoalveolar lavage fluid from children with cystic fibrosis
    William T Harris
    Division of Pulmonology, Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Pediatr Pulmonol 44:1057-64. 2009
    ..However, few data link TGF-beta(1) protein levels and clinical markers of CF lung disease severity...
  44. ncbi Comparative acute lung inflammation induced by atmospheric PM and size-fractionated tire particles
    Paride Mantecca
    Department of Environmental Science, POLARIS Research Center, University of Milano Bicocca, 1 piazza della Scienza, Milan 20126, Italy
    Toxicol Lett 198:244-54. 2010
    ..These results provide evidence of variable inflammatory mechanisms in mouse lungs in response to both urban PM and tire particles...
  45. ncbi Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital, North Terrace, Adelaide, South Australia 5001, Australia
    Immunol Cell Biol 81:289-96. 2003
    ....
  46. pmc Inhalation exposure study of titanium dioxide nanoparticles with a primary particle size of 2 to 5 nm
    Vicki H Grassian
    Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Environ Health Perspect 115:397-402. 2007
    ..Nanotechnology offers great promise in many industrial applications. However, little is known about the health effects of manufactured nanoparticles, the building blocks of nanomaterials...
  47. ncbi Oxidative stress, inflammation, and DNA damage in rats after intratracheal instillation or oral exposure to ambient air and wood smoke particulate matter
    Pernille Høgh Danielsen
    Section of Environmental Health, Department of Public Health, University of Copenhagen, DK 1014 Copenhagen, Denmark
    Toxicol Sci 118:574-85. 2010
    ..These results indicate that WSPM and CB exert the strongest effect in terms of oxidative stress-induced response, inflammation, and genotoxicity in the organ closest to the port of entry...
  48. ncbi Impact of a Mycobacterium tuberculosis-specific interferon-γ release assay in bronchoalveolar lavage fluid for a rapid diagnosis of tuberculosis
    C Jafari
    Division of Clinical Infectious Diseases, Research Center Borstel, Borstel, Germany
    J Intern Med 270:254-62. 2011
    ..Evaluation of different methods for an initial treatment decision in individuals with suspected pulmonary tuberculosis...
  49. pmc Inhibition of airway remodeling in IL-5-deficient mice
    Jae Youn Cho
    Department of Medicine, University of California San Diego, La Jolla, California 92093 0635, USA
    J Clin Invest 113:551-60. 2004
    ..Increased airway epithelial expression of alphaVbeta6 integrin may contribute to the increased activation of latent TGF-beta. These results suggest an important role for IL-5, eosinophils, alphaVbeta6, and TGF-beta in airway remodeling...
  50. ncbi Carbon fullerenes (C60s) can induce inflammatory responses in the lung of mice
    Eun Jung Park
    College of Pharmacy, Dongduk Women s University, 23 1, Wolgok dong, Seongbuk gu, Seoul 136 714, Korea
    Toxicol Appl Pharmacol 244:226-33. 2010
    ..Furthermore, cell infiltration and expression of tissue damage related genes in lung tissue were constantly observed during the experiment period. Based on this, C60s may induce inflammatory responses in the lung of mice...
  51. ncbi Single-walled and multi-walled carbon nanotubes promote allergic immune responses in mice
    Unni C Nygaard
    Division of Environmental Medicine, Norwegian Institute of Public Health, NO 0403 Oslo, Norway
    Toxicol Sci 109:113-23. 2009
    ..IgE, IgG1, and IgG2a antibodies in serum and the numbers of inflammatory cells and cytokine levels in bronchoalveolar lavage fluid (BALF) were determined...
  52. pmc Dendritic cell subsets in human bronchoalveolar lavage fluid after segmental allergen challenge
    Kai Bratke
    Department of Pneumology, University of Rostock, Rostock, Germany
    Thorax 62:168-75. 2007
    Dendritic cells control pulmonary immune reactions. Characteristics of dendritic cells in human bronchoalveolar lavage fluid (BALF) after allergen challenge are unknown.
  53. pmc Endothelium-derived Toll-like receptor-4 is the key molecule in LPS-induced neutrophil sequestration into lungs
    Graciela Andonegui
    Immunology Research Group, Department of Physiology and Biophysics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Clin Invest 111:1011-20. 2003
    ..In conclusion, our data challenge the view that LPS directly activates neutrophils to trap in lungs and suggest a far more important role than previously appreciated for the endothelial cells...
  54. pmc Prolonged allergen challenge in mice leads to persistent airway remodelling
    S J McMillan
    Leukocyte Biology Section, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London, UK
    Clin Exp Allergy 34:497-507. 2004
    ..Current animal models of allergen-induced airway inflammation generally concentrate on the acute inflammation following allergen exposure and fail to mimic all of these features...
  55. ncbi Azithromycin improves macrophage phagocytic function and expression of mannose receptor in chronic obstructive pulmonary disease
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital and Lung Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia
    Am J Respir Crit Care Med 178:139-48. 2008
    ..We have previously reported that low-dose azithromycin improved alveolar macrophage (AM) phagocytic function in vitro...
  56. ncbi Involvement of distal airways in a chronic model of experimental asthma
    M Wegmann
    Department of Clinical Chemistry and Molecular Diagnostics, Hospital of the Philipps University, Marburg, Germany
    Clin Exp Allergy 35:1263-71. 2005
    ..Bronchial asthma is characterized by chronic airway inflammation and airway remodelling which occurs in both proximal and distal airways. These changes are associated with development of airway hyper-responsiveness and airflow limitation...
  57. pmc Macrophages directly contribute to the exaggerated inflammatory response in cystic fibrosis transmembrane conductance regulator-/- mice
    Emanuela M Bruscia
    Yale University School of Medicine, Department of Pediatrics, 333 Cedar Street, PO Box 208064, New Haven, CT 06520 8035, USA
    Am J Respir Cell Mol Biol 40:295-304. 2009
    ..WT mice, CF mice had increased numbers of neutrophils and increased proinflammatory cytokines in their bronchoalveolar lavage fluid after LPS exposure...
  58. pmc Airway inflammation and bronchial bacterial colonization in chronic obstructive pulmonary disease
    Sanjay Sethi
    Division of Pulmonary Critical Care and Sleep Medicine, and Division of Infectious Diseases, Department of Medicine, University at Buffalo, Buffalo, NY, USA
    Am J Respir Crit Care Med 173:991-8. 2006
    ..This "colonization" of the tracheobronchial tree, currently believed to be innocuous, could serve as an inflammatory stimulus, independent of current tobacco smoke exposure...
  59. ncbi Use of bronchoalveolar lavage to detect respiratory tract toxicity of inhaled material
    Rogene F Henderson
    Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr SE, Albuquerque, NM 87108, USA
    Exp Toxicol Pathol 57:155-9. 2005
    ....
  60. ncbi Alveolar macrophages have a protective antiinflammatory role during murine pneumococcal pneumonia
    Sylvia Knapp
    Laboratory of Experimental Internal Medicine, Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands
    Am J Respir Crit Care Med 167:171-9. 2003
    ..These data suggest that AMs are indispensable in the host response to pneumococcal pneumonia by means of their capacity to modulate inflammation, possibly via elimination of apoptotic PMNs...
  61. ncbi Oxygen radicals in influenza-induced pathogenesis and treatment with pyran polymer-conjugated SOD
    T Oda
    Department of Microbiology, Kumamoto University Medical School, Japan
    Science 244:974-6. 1989
    ....
  62. ncbi Differences in interleukin-8 and tumor necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma
    V M Keatings
    Department of Thoracic Medicine, National Heart and Lung Institute, London, United Kingdom
    Am J Respir Crit Care Med 153:530-4. 1996
    ..Interleukin-8, but not TNF alpha, was significantly higher in the COPD group than in the asthmatic group. We conclude that the cytokines TNF alpha and IL-8 may be involved in the inflammation in COPD...
  63. pmc Interferon gamma induction of pulmonary emphysema in the adult murine lung
    Z Wang
    Department of Internal Medicine, Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, New Haven, CT 06520 8057, USA
    J Exp Med 192:1587-600. 2000
    ..IFN-gamma causes emphysema and alterations in pulmonary protease/antiprotease balance when expressed in pulmonary tissues...
  64. ncbi Particulate matter induces translocation of IL-6 from the lung to the systemic circulation
    Takashi Kido
    James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, St Paul s Hospital, Child and Family Research Institute, University of British Columbia, Vancouver, Canada
    Am J Respir Cell Mol Biol 44:197-204. 2011
    ..Concentrations of IL-6 were measured in bronchoalveolar lavage fluid (BALF) and serum samples...
  65. pmc Effects of prenatal exposure to surface-coated nanosized titanium dioxide (UV-Titan). A study in mice
    Karin S Hougaard
    National Research Centre for the Working Environment, Copenhagen Ø, Denmark
    Part Fibre Toxicol 7:16. 2010
    ..Consequently, also toxicological properties may change compared to the parent compound. We examined developmental and neurobehavioral effects following maternal exposure to a nanoparticulate UV-filter (UV-titan L181)...
  66. pmc Detection and quantitation of eosinophils in the murine respiratory tract by flow cytometry
    Whitney W Stevens
    The Carter Immunology Center, UVa Health Sciences Center, Charlottesville, VA 22908, United States
    J Immunol Methods 327:63-74. 2007
    ..In this study, we describe a new flow cytometry-based assay to enumerate eosinophils present in murine bronchoalveolar lavage fluid (BAL) and lung parenchyma obtained from the normal/non-inflamed respiratory tract, following ..
  67. ncbi Development of a short-term inhalation test in the rat using nano-titanium dioxide as a model substance
    Lan Ma-Hock
    Department of Product Safety, BASF SE, Ludwigshafen, Germany
    Inhal Toxicol 21:102-18. 2009
    ..Lung inflammation was associated with dose-dependent increases in bronchoalveolar lavage fluid (BALF) total cell and neutrophil counts, total protein content, enzyme activities and levels of a ..
  68. pmc Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
    Prasanna D Khot
    Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    BMC Infect Dis 8:73. 2008
    ..It is important to adopt quality control measures in PCR assays to address false positives and negatives which can hinder accurate evaluation of diagnostic performance...
  69. ncbi Inhibition of phosphodiesterase 4 attenuates airway hyperresponsiveness and airway inflammation in a model of secondary allergen challenge
    A Kanehiro
    Division of Basic Sciences, Department of Pediatrics and Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    Am J Respir Crit Care Med 163:173-84. 2001
    ..RL significantly increased as did the number of lung inflammatory cells and IL-4 and IL-5 production in bronchoalveolar lavage fluid (BALF)...
  70. ncbi Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways
    Masahide Miyamoto
    Lung Pharmacology Group, Department of Respiratory Medicine and Allergology, Institute of Internal Medicine, Goteborg University, Gothenburg, Sweden
    J Immunol 170:4665-72. 2003
    ..These IL-17-related mechanisms constitute potential targets for pharmacotherapy against exaggerated neutrophil recruitment in airway disease...
  71. ncbi Inhibition of chronic airway inflammation and remodeling by galectin-3 gene therapy in a murine model
    Esther Lopez
    Immunology Department, Fundacion Jimenez Diaz Capio, Madrid, Spain
    J Immunol 176:1943-50. 2006
    ..Eosinophils were counted in blood and bronchoalveolar lavage fluid. Real-time PCR was used to measure Gal-3 and cytokine mRNA expression in lung...
  72. ncbi Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer
    Joanna Domagała-Kulawik
    Department of Pneumonology, Warsaw Medical Academy, Banacha 1a, 02 097, Warsaw, Poland
    Arch Immunol Ther Exp (Warsz) 54:143-7. 2006
    ..The aim of this study was to evaluate TGF-beta1 levels and lymphocyte subsets in the broncholaveolar lavage fluid (BALF) of patients with primary lung cancer and to analyze the interdependence of these parameters...
  73. pmc Schistosoma mansoni antigens modulate experimental allergic asthma in a murine model: a major role for CD4+ CD25+ Foxp3+ T cells independent of interleukin-10
    Lucila G G Pacifico
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Pampulha, Belo Horizonte, MG, Brazil 31270 901
    Infect Immun 77:98-107. 2009
    ..The number of eosinophils in bronchoalveolar lavage fluid was higher in the asthmatic group than in asthmatic mice infected with S...
  74. ncbi Therapeutic role for mannose-binding lectin in cigarette smoke-induced lung inflammation? Evidence from a murine model
    Sandra Hodge
    Department of Thoracic Medicine, Royal Adelaide Hospital and Lung Research Laboratory, Hanson Institute, Adelaide, South Australia, Australia
    Am J Respir Cell Mol Biol 42:235-42. 2010
    ..We provide evidence for Rac1/2/3 involvement in the MBL-mediated improvement in efferocytosis, and a rationale for investigating MBL as a supplement to existing therapies in smoking-related lung inflammation...
  75. ncbi Key role for respiratory CD103(+) dendritic cells, IFN-γ, and IL-17 in protection against Streptococcus pneumoniae infection in response to α-galactosylceramide
    Stoyan Ivanov
    Institut Pasteur de Lille, Centre d Infection et d Immunité de Lille, France
    J Infect Dis 206:723-34. 2012
    ..We undertook the present study to characterize mechanisms leading to α-GalCer-mediated protection against lethal infection with Streptococcus pneumoniae serotype 1, a major respiratory pathogen in humans...
  76. ncbi Pulmonary alveolar proteinosis
    Bruce C Trapnell
    Division of Pulmonary Biology, Children s Hospital Medical Center, Cincinnati, OH 45229, USA
    N Engl J Med 349:2527-39. 2003
  77. pmc Nicotine exerts an anti-inflammatory effect in a murine model of acute lung injury
    Jon Mabley
    School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK
    Inflammation 34:231-7. 2011
    ..2 or 0.4 mg/kg, sc). After 24 h, bronchoalveolar lavage fluid (BALF) was obtained to measure leukocyte infiltration, lung edema, and pro-inflammatory chemokine (MIP-..
  78. ncbi Prevention of influenza-induced lung injury in mice overexpressing extracellular superoxide dismutase
    H B Suliman
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Physiol Lung Cell Mol Physiol 280:L69-78. 2001
    ..measured by significant blunting of interferon-gamma induction, reduced cell count and total protein in bronchoalveolar lavage fluid, reduced levels of lung nitrite/nitrate nitrotyrosine, and markedly reduced lung pathology...
  79. ncbi The acute toxic effects of particulate matter in mouse lung are related to size and season of collection
    Francesca Farina
    Department of Experimental Medicine, POLARIS Research Center, University of Milano Bicocca, 48 via Cadore, Monza 20052, Italy
    Toxicol Lett 202:209-17. 2011
    ..The bronchoalveolar lavage fluid (BALf) and the lung parenchyma were screened for different markers of inflammation and cytotoxicity...
  80. ncbi Microbiology of bronchoalveolar lavage fluid in children with acute nonresponding or recurrent community-acquired pneumonia: identification of nontypeable Haemophilus influenzae as a major pathogen
    Iris De Schutter
    Department of Pediatric Pulmonology, CF Clinic and Pediatric Infectious Diseases, niversitair Ziekenhuis Brussel UZ Brussel, Brussels
    Clin Infect Dis 52:1437-44. 2011
    ..Precise etiologic diagnosis in pediatric community-acquired pneumonia (CAP) remains challenging...
  81. ncbi Allergen provocation increases TH2-cytokines and FOXP3 expression in the asthmatic lung
    S Thunberg
    Clinical Immunology and Allergy Unit, Department of Medicine, Karolinska Institute, University Hospital, Stockholm, Sweden
    Allergy 65:311-8. 2010
    ..The objective of this study was to investigate local and systemic regulatory mechanisms in the early asthmatic response to bronchial allergen provocation...
  82. pmc Severe pneumonia with Leptotrichia sp. detected predominantly in bronchoalveolar lavage fluid by use of 16S rRNA gene sequencing analysis
    Toshinori Kawanami
    Department of Respiratory Disease, University of Occupational and Environmental Health, Fukuoka, Japan
    J Clin Microbiol 47:496-8. 2009
    ..This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis...
  83. pmc Alveolar macrophages stimulate enhanced cytokine production by pulmonary CD4+ T-lymphocytes in an exacerbation of murine chronic asthma
    Cristan Herbert
    Inflammation and Infection Research Centre, Schoolof Medical Sciences, University of New South Wales, Sydney, Australia
    Am J Pathol 177:1657-64. 2010
    ..We conclude that in an acute exacerbation of asthma, enhanced cytokine expression by AM may play a critical role in triggering increased expression of cytokines by pulmonary CD4(+) T-lymphocytes...
  84. pmc Respiratory reovirus 1/L induction of intraluminal fibrosis, a model of bronchiolitis obliterans organizing pneumonia, is dependent on T lymphocytes
    Elizabeth I Majeski
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Am J Pathol 163:1467-79. 2003
    ....
  85. pmc Eosinophil and T cell markers predict functional decline in COPD patients
    JEANINE M D'ARMIENTO
    Department of Medicine, Columbia University, New York, NY, USA
    Respir Res 10:113. 2009
    ..We therefore measured cytokine levels in the lung lavage fluid and plasma of COPD patients in order to determine if the levels of T cell or eosinophil related cytokines were predictive of the future course of the disease...
  86. ncbi False-positive serum and bronchoalveolar lavage Aspergillus galactomannan assays caused by different antibiotics
    Viboon Boonsarngsuk
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
    Scand J Infect Dis 42:461-8. 2010
    ..In summary, this study demonstrates the false-positive GM levels in serum and BAL caused by beta-lactam antibiotics that are commonly used in general practice. Physicians should be aware of this possible interference...
  87. pmc Toll-like receptor 2 regulates organic dust-induced airway inflammation
    Jill A Poole
    Omaha Veterans Affairs Medical Center, Omaha, Nebraska, USA
    Am J Respir Cell Mol Biol 45:711-9. 2011
    ..Next, using an established mouse model of intranasal inhalation challenge, we analyzed bronchoalveolar lavage fluid and lung tissue in TLR2-deficient and wild-type (WT) mice after single and repetitive DE challenge...
  88. ncbi Proteomic analysis of inflammatory biomarkers in bronchoalveolar lavage
    Carlos de Torre
    Critical Care Medicine Department, Clinical Center, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA
    Proteomics 6:3949-57. 2006
    ..This pattern was also found in ARDS BAL after immunoblot analysis. These approaches will be useful to improve current methods of monitoring lung inflammation and to identify new therapeutic targets...
  89. ncbi CCL18 as an indicator of pulmonary fibrotic activity in idiopathic interstitial pneumonias and systemic sclerosis
    Antje Prasse
    University Hospital Freiburg, Freiburg, Germany
    Arthritis Rheum 56:1685-93. 2007
    ..In this study the role of CCL18 as a biomarker of disease activity in idiopathic interstitial pneumonias (IIPs) and systemic sclerosis (SSc) with lung involvement was evaluated...
  90. ncbi TGF-beta and Smad3 signaling link inflammation to chronic fibrogenesis
    Philippe Bonniaud
    Department of Pathology and Molecular Medicine, Center for Gene Therapeutics, McMaster University, Hamilton, Ontario, Canada
    J Immunol 175:5390-5. 2005
    ..KO and WT animals demonstrated a similar inflammatory response to overexpression of IL-1beta indicating that inflammation must link to the Smad3 pathway, likely through TGF-beta, to induce progressive fibrosis...
  91. pmc Blocking the leukotriene B4 receptor 1 inhibits late-phase airway responses in established disease
    Koichi Waseda
    Department of Hematology, Oncology, Allergy and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
    Am J Respir Cell Mol Biol 45:851-7. 2011
    ..These data identify the importance of the LTB4-BLT1 pathway in the development of late-phase, allergen-induced airway responsiveness after secondary airway challenge in mice with established airway disease...
  92. ncbi Levels of interferon-gamma and interleukin-2 receptor-alpha for bronchoalveolar lavage fluid and serum were correlated with clinical grade and treatment of pulmonary tuberculosis
    T C Y Tsao
    Division of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan
    Int J Tuberc Lung Dis 6:720-7. 2002
    ..for interferon-gamma (IFN-gamma) and soluble interleukin-2 receptor-alpha (sIL-2R-alpha) levels in bronchoalveolar lavage fluid (BALF), and clinical grade of pulmonary tuberculosis (TB), which is determined by factors such as ..
  93. ncbi Thickness of multiwalled carbon nanotubes affects their lung toxicity
    Ivana Fenoglio
    Dipartimento di Chimica, Universita degli Studi di Torino, Via P Giuria 7, Torino 10125, Italy
    Chem Res Toxicol 25:74-82. 2012
    ..The data reported herein suggest that the nanotube diameter is an important parameter to be considered in the toxicological assessment of CNTs...
  94. ncbi Zinc uptake by Streptococcus pneumoniae depends on both AdcA and AdcAII and is essential for normal bacterial morphology and virulence
    Lucie Bayle
    CEA, Institut de Biologie Structurale Jean Pierre Ebel, Grenoble, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
    Mol Microbiol 82:904-16. 2011
    ..Dual deletion of adcA and adcAII strongly impaired growth of the pneumococcus in bronchoalveolar lavage fluid and human serum, and prevented S...
  95. ncbi Bronchoscopic evaluation of severe asthma. Persistent inflammation associated with high dose glucocorticoids
    S E Wenzel
    Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado, USA
    Am J Respir Crit Care Med 156:737-43. 1997
    ..b>Bronchoalveolar lavage fluid was analyzed for cytology and inflammatory mediators...
  96. ncbi Evaluation of inhaled carbon monoxide as an anti-inflammatory therapy in a nonhuman primate model of lung inflammation
    Leah A Mitchell
    Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA
    Am J Physiol Lung Cell Mol Physiol 299:L891-7. 2010
    ..CO exposure (500 ppm, 6 h) following LPS inhalation decreased TNF-α release in bronchoalveolar lavage fluid but did not affect IL-6 or IL-8 release...
  97. ncbi CCL18 production is decreased in alveolar macrophages from cigarette smokers
    Florian Kollert
    Department of Pneumology, Medical Center, Albert Ludwigs University, Freiburg, Germany
    Inflammation 32:163-8. 2009
    ..Thus this article gives further evidence that chronic cigarette smoking alters the phenotype of AM and that the M2 marker CCL18 is down-regulated in smokers macrophages...
  98. pmc Comparative proteomics and pulmonary toxicity of instilled single-walled carbon nanotubes, crocidolite asbestos, and ultrafine carbon black in mice
    Justin G Teeguarden
    Pacific Northwest National Laboratory, Richland, Washington 99352, USA
    Toxicol Sci 120:123-35. 2011
    ..Two high-sensitivity markers of inflammation, one (S100a9) observed in humans exposed to AB, were found and may be promising biomarkers of human response to SWCNT exposure...
  99. ncbi In vivo and in vitro effects of salbutamol on alveolar epithelial repair in acute lung injury
    G D Perkins
    Warwick Medical School, University of Warwick, Coventry, UK
    Thorax 63:215-20. 2008
    ..One possible explanation for the delay in change in extravascular lung water is the time required for salbutamol to stimulate alveolar epithelial repair...
  100. ncbi Evaluation of bronchoalveolar lavage fluid from ARDS patients with regard to apoptosis
    Keu Sung Lee
    Department of Pulmonary and Critical Care Medicine, Ajou University School of Medicine, Suwon, South Korea
    Respir Med 102:464-9. 2008
    ..Apoptosis is thought to play an important role in the development of acute respiratory distress syndrome (ARDS). We evaluated the bronchoalveolar lavage (BAL) fluid from ARDS patients focusing on apoptosis...
  101. ncbi Pepsin in bronchoalveolar lavage fluid: a specific and sensitive method of diagnosing gastro-oesophageal reflux-related pulmonary aspiration
    Stephen Farrell
    Department of Child Health, Queen s University Belfast, Royal Victoria Hospital, Institute of Clinical Science, Belfast, BT12 6BJ Northern Ireland, UK
    J Pediatr Surg 41:289-93. 2006
    ..A specific marker of GOR-related aspiration should originate in the stomach, but not the lung. An assay to detect gastric pepsin in the bronchoalveolar lavage (BAL) of children was developed and validated...

Research Grants63

  1. Effect of Macrolide Treatment on a Novel Pathway of Neutrophilic Inflammation in
    J Edwin Blalock; Fiscal Year: 2010
    ..We have also found that PGP is present in bronchoalveolar lavage fluid (BALF) and/or sputum from most COPD patients but not controls or asthmatics...
  2. Characterizing TLR Function of Human Dendritic Cells in the Elderly
    Alexander Panda; Fiscal Year: 2012
    ..his independent research program, a pilot study of TLR function in lung-derived alveolar macrophages in bronchoalveolar lavage fluid from young and older individuals will be carried out to obtain pilot data...
  3. Mechanisms of adaptive immunity to P. aeruginosa in the lung
    Gregory P Priebe; Fiscal Year: 2013
    ..the cytokine IL- 17 is produced by a greater number of lung T cells, can be found at higher levels in bronchoalveolar lavage fluid compared with control vaccines, and is associated with more rapid recruitment of neutrophils to the ..
  4. Periostin Regulation of Lung Fibrosis
    Bethany B Moore; Fiscal Year: 2013
    ..via which periostin and TGFb regulate each other and the function of lung mesenchymal cells Aim 4) To determine whether periostin levels in plasma or bronchoalveolar lavage fluid of IPF patients correlate with disease progression
  5. Lung carcinogenesis: Chemoprevention by Indole-3-carbinol
    Fekadu Kassie; Fiscal Year: 2013
    ..using antibody-based protein microarray approach, differential proteomic patterns in the serum and bronchoalveolar lavage fluid (BALF) of mice treated with NNK, I3C, or NNK and I3C and assess functional consequences of alterations ..
  6. Dysregulated Pathways in Pulmonary Fibrosis
    Keith C Olsen; Fiscal Year: 2012
    ..In Aim 1, the levels of PPAR? and TG2 protein and mRNA in human IPF lung samples and bronchoalveolar lavage fluid will be examined...
  7. SP-D as an immunomodulatory protein in acute rejection in lung transplantation
    KAREN D SIMS; Fiscal Year: 2010
    ..SNPs with alterations in the biochemical characteristics of SP-D in lung transplant recipient-derived bronchoalveolar lavage fluid (BALF)...
  8. Role of T Regulatory Cells in Preventing Human BOS
    Aric L Gregson; Fiscal Year: 2013
    ..We have recently found that lung transplant recipients with increased frequencies of Treg within the bronchoalveolar lavage fluid do not develop BOS...
  9. Allogeneic Human Mesenchymal Stem Cells for the Treatment of Acute Lung Injury
    Michael A Matthay; Fiscal Year: 2013
    ..We are also proposing two ancillary studies, both of which will use samples of plasma and bronchoalveolar lavage fluid (BALF) from patients in the clinical trial to provide mechanistic insights into how human MSC reduce ..
  10. Viral Pathogenesis of Early Cystic Fibrosis Lung Disease
    SARATH CHANDRA RANGANATHAN; Fiscal Year: 2013
    ..b>Bronchoalveolar lavage fluid and pharyngeal secretions will be collected at predetermined times during infancy, and highly sensitive ..
  11. INNATE IMMUNE REGULATION OF PRIMARY GRAFT DYSFUNCTION
    Howard J Huang; Fiscal Year: 2013
    ..molecular patterns released by sterile tissue injury, which have been reported in serum and bronchoalveolar lavage fluid of human lung transplant recipients...
  12. Immunophilins regulate SOC entry channels in pulmonary endothelial cells
    Donna L Cioffi; Fiscal Year: 2013
    ..in vitro;and the isolated, perfused lung model measuring filtration coefficient (Kf), wet/dry lung weight ratio, alveolar fluid volume fraction and albumin permeability (Evan's Blue dye in bronchoalveolar lavage fluid) in situ.
  13. IL-13 and IL-17 dynamics in the asthmatic airway
    Dean Sheppard; Fiscal Year: 2013
    ..basic characterization of airway physiology, evidence for IL-13 and IL-17 bioactivity, and samples of bronchoalveolar lavage fluid, epithelial brushings and airway biopsies for use in each of the 3 projects...
  14. Impact of CCR5 inhibition on Sarcoid Immunophenotypes
    KEVIN FITZGERALD GIBSON; Fiscal Year: 2013
    ..specimens (mediastinoscopy and endobronchial ultrasound guided fine needle aspirations [EBUS-FNA]), bronchoalveolar lavage fluid (BALF), skin biopsies, and peripheral blood of sarcoidosis subjects, along with detailed subject ..
  15. Obesity and Environmental Lung Disease: The Role of Chemerin
    RICHARD ALAN JOHNSTON; Fiscal Year: 2013
    ..Our preliminary data demonstrate that (1) O3-induced increases in the levels of chemerin in the bronchoalveolar lavage fluid are greater in obese as compared to lean, wild-type mice and (2) O3-induced pulmonary inflammation is ..
  16. The role of common genetic variation in the Fas pathway in acute lung injury
    BRADFORD JOSEPH GLAVAN; Fiscal Year: 2010
    ..studies have shown that adverse outcomes in patients with ALI are associated with increased plasma and bronchoalveolar lavage fluid levels of soluble FasL...
  17. Aging related susceptibility to lung injury - remodeling and oxidative stress
    Jesse Roman; Fiscal Year: 2012
    ..lungs, by determining the impact of nutritional interventions as preventive strategies, and by testing for evidence of this pro-fibrotic state in the bronchoalveolar lavage fluid of young versus elderly humans who are otherwise healthy.
  18. Resolution of ozone induced lung injury requires gamma/delta T cells
    Joel A Mathews; Fiscal Year: 2013
    ..will be exposed to subacute O3 and a time course for the recruitment of innate immune cells to both the bronchoalveolar lavage fluid and the lung tissue will assessed and compared to Wt mice...
  19. Role of Regulatory CD8 T Cells in Murine Asthma
    Roger S Thrall; Fiscal Year: 2013
    ..AAD to LIT and that this response is localized to cells in respiratory (hilar lymph node, lung tissue, bronchoalveolar lavage fluid) as compared to systemic (inguinal lymph node, spleen) tissue compartments: a...
  20. Obesity and Allergic Airway Hyperresponsiveness
    RICHARD ALAN JOHNSTON; Fiscal Year: 2013
    ..lean, wild-type mice exhibit increased airway responsiveness to methacholine but decreased numbers of bronchoalveolar lavage fluid eosinophils...
  21. Virus Detection Using Microarrays and Characterization of a Novel Cardiovirus
    CHARLES YEN CHIU; Fiscal Year: 2010
    ..For the proposed research, the candidate will use the Virochip to retrospectively analyze samples of bronchoalveolar lavage fluid from 33 patients with acute respiratory distress syndrome (Aim 1a) and prospectively analyze ..
  22. Extracellular Matrix Synthesis and Turnover in Asthma
    Mark A Aronica; Fiscal Year: 2010
    ..HA levels are increased in the bronchoalveolar lavage fluid (BALF) from asthmatics, and their level is associated with the severity of disease...
  23. Genomic Analysis of Immunity and Lung Inflammation in HIV Infection
    George M Weinstock; Fiscal Year: 2013
    ..and virome in patients on longterm HAART by measuring acellular HIV and cellular HIV RNA and HIV DNA in bronchoalveolar lavage fluid and blood...
  24. Molecular biomarkers of airway and lung linking COPD and lung cancer
    AVRUM E SPIRA; Fiscal Year: 2013
    ..Similarly, the degree of lung function impairment, extent of emphysema, and expression of proteins in bronchoalveolar lavage fluid differ in women with COPD compared to men with this disease...
  25. Targeting epithelial cells to treat pulmonary fibrosis
    Dean Sheppard; Fiscal Year: 2013
    ..in human lung, and developing read-outs of drug effectiveness that can be detected in blood, bronchoalveolar lavage fluid or alveolar macrophages, the work performed should lay the groundwork for clinical trials of the most ..
  26. Th17 inflammation is responsible for mucous metaplasia in COPD
    Victor Kim; Fiscal Year: 2013
    ..will obtain bronchoscopic mucosal biopsies and epithelial brushings to quantitate mucous metaplasia and bronchoalveolar lavage fluid to measure Th17 cytokines...
  27. Sytems Analysis of Nanoparticle Biocompatibility
    Brian D Thrall; Fiscal Year: 2011
    ..In addition, the approach and biomarkers developed from this work will provide a screening platform that can be deployed to a variety of nanomaterials in the future. ..
  28. Genotypic and functional properties of HIV-1 Nef clinical isolates in PAH-HIV
    Priscilla Y Hsue; Fiscal Year: 2013
    ..Our research tem, with a combination of basic and clinician scientists is well poised to address how the nef viral protein and immune dysregulation are contributing factors to this lung complication of HIV. ..
  29. Lipoxins in Severe Asthma (LIPSA)
    Bruce D Levy; Fiscal Year: 2012
    ..In CADET I, we propose to utilize samples of blood and bronchoalveolar lavage fluid from individuals with severe asthma for two specific aims to: }} Validate soluble epoxide hydrolase ..
  30. MECHANISMS OF AIRFLOW OBSTRUCTION
    Julian Solway; Fiscal Year: 1992
    ..endings) will be assessed at various airway sites by: (a) measuring arterio-venous differences and bronchoalveolar lavage fluid contents of histamine, leukotriene D4, prostaglandin D2, and substance P, before and during HIB in the ..
  31. Metabolic profiles of COPD phenotypes
    RUSSELL PAUL BOWLER; Fiscal Year: 2013
    ..The first step (Aim 1) of the project will be to identify novel metabolic pathways in plasma and bronchoalveolar lavage fluid that are dysregulated in COPD and emphysema...
  32. Impaired AMPK activity contributes to vascular pathogenesis associated with PH
    Judy R Creighton; Fiscal Year: 2013
    ..2] Changes in AMPK ????? expression patterns correlate with segment specific vascular disease in PH. ..
  33. Virus Electrodes for Fungal Pathogen Detection
    Gary Fujii; Fiscal Year: 2009
    ..aimed at demonstrating the ability of the viratrode to rapidly test a sample of blood, serum, urine or bronchoalveolar lavage fluid for the presence of an Aspergillus specific marker...
  34. Dissecting the Pathways Leading to Injury in Lung Transplantation
    David Neujahr; Fiscal Year: 2013
    ..In this proposal we will determine the events which precipitate lung injury in hopes of highlighting new therapeutic strategies to help patients with transplanted lungs live longer. ..
  35. Modulation of innate immunity in lung transplantation
    Sadis Matalon; Fiscal Year: 2005
    ..Herein, we are proposing to isolate SP-A and AMs from the bronchoalveolar lavage fluid (BALF) of patients participating in this clinical trial to identify differences in the ability of AMs ..
  36. Matrix Metalloproteinases and PPAR Gamma in Sarcoidosis
    Daniel Culver; Fiscal Year: 2009
    ..12 and their endogenous inhibitors (tissue inhibitors of metalloproteinases- TIMPs) in granulomas and bronchoalveolar lavage fluid. Samples will all be from patients with well-demarcated clinical phenotypes (progressed versus improved)..
  37. Targeted DNA extraction for Tuberculosis PCR from sputum
    Eric Houpt; Fiscal Year: 2007
    ..inadequacy of existing diagnostic methods for Tb (smear and culture), shows the utility of Tb PCR on bronchoalveolar lavage fluid, and demonstrates increased PCR sensitivity with DNA capture...
  38. PNEUMOCYSTIS CARINII IN CONTINOUS AXENIC CULTURE
    ALLEN CLARKSON JR; Fiscal Year: 2001
    ..A library of 50 isolates of human-derived P. carinii will be obtained using bronchoalveolar lavage fluid from patients with P. carinii pneumonia...
  39. Ventilator-Induced Lung Injury in Children
    Sabrina Heidemann; Fiscal Year: 2004
    ..The results of this study may serve as the basis for a multicenter clinical trial. ..
  40. ASTHMA--ALLERGY AND AUTOIMMUNITY
    ENG TAN; Fiscal Year: 1990
    ..Project I examines the role of tissue kallikrein in asthma and is targeted at studies on bronchoalveolar lavage fluid. The identity and source of glandular kallikrein will be determined including its possible role in ..
  41. Intrapulmonary Gene Transfer Using Compacted DNA
    Mark Cooper; Fiscal Year: 2001
    ..SECR-targeted PLASmin complexes for intrapulmonary gene delivery; and iii) determine plasma and bronchoalveolar lavage fluid levels of human factor IX and alpha(1)-antitrypsin after intrapulmonary administration of non-targeted ..
  42. BIOLOGY OF IL-16 IN IL-1680 AND IL-1614 TRANSGENIC MICE
    Geoffrey Chupp; Fiscal Year: 2003
    ..by bronchial epithelium which induces motile responses in CD bearing cells, and is detectable in bronchoalveolar lavage fluid of asthmatics...
  43. TOXICOLOGY OF COMBUSTION GENERATED ACID AEROSOLS
    MARY AMDUR; Fiscal Year: 1991
    ..Special emphasis is placed on determination of H2S04, both as free H2S04 and as a layer on ultrafine aerosols, and on how H2S04 formation relates to composition of coal and to combustion conditions...
  44. BASIC MECHANISMS OF RE-EXPANSION PULMONARY EDEMA
    Robert Jackson; Fiscal Year: 1991
    ..It will serve as a model for testing experimental interventions in acute lung injury, since the internal control lung will greatly minimize consumption of experimental animals and no exogenous toxicant need be administered...
  45. PHYSIOLOGICAL MECHANISMS OF OXYGEN TOXICITY
    John Newman; Fiscal Year: 1992
    ..of multiple variables of lung vascular and airway function as well as sampling of blood, lung lymph, bronchoalveolar lavage fluid and lung biopsy tissue for biochemical markers of toxicity...
  46. BioRad Galactomannan EIA for Diagnosis of Aspergillosis
    Kieren Marr; Fiscal Year: 2007
    ..that impact levels of circulating GM, and to determine the role of GM EIA applied to non-blood fluids (bronchoalveolar lavage fluid and urine)...
  47. Viral-induced T Cell Responses in COPD Exacerbation
    Farrah Kheradmand; Fiscal Year: 2009
    ..We have also detected significant levels of IL-4, the canonical Th2 cytokine, in bronchoalveolar lavage fluid (BAL) of subjects with COPD exacerbation that required mechanical ventilation while very few subjects ..
  48. Vaccine Induced Mucosal Protection Against TB Infection
    DANIEL HOFT; Fiscal Year: 2005
    ..1 month after the final booster vaccination, lung lymphocytes, spleen cells and bronchoalveolar lavage fluid will be harvested and studied for antigen specific IFN(, CTL and slgA/IgG responses...
  49. EOSINOPHIL INFLUX INTO THE LUNG IN NOCTURNAL ASTHMA
    Monica Kraft; Fiscal Year: 2000
    ..granulocyte-macrophage colony stimulating factor (GMCSF) MRNA expression and product in lung tissue and bronchoalveolar lavage fluid at four time points will be evaluated...
  50. Immune response to Pneumocystis in simian model of AIDS
    Karen Norris; Fiscal Year: 2009
    ..We will measure cellular composition and inflammatory mediators in bronchoalveolar lavage fluid and correlate changes in local immune responses with alterations in lung function...
  51. IMMUNE AND THERAPEUTIC SUPPRESSION OF HIV IN THE LUNG
    RICHARD KORNBLUTH; Fiscal Year: 2000
    ..4) Finally, the cellular factors (e.g., from CD8 + T cell) and soluble factors present in bronchoalveolar lavage fluid (e.g...
  52. FUNCTIONS OF PULMONARY ALVEOLAR TYPE II CELLS IN VITRO
    Robert Mason; Fiscal Year: 2000
    ..of growth factors that stimulate proliferation in vitro and are potentially important in vivo are bronchoalveolar lavage fluid and media conditioned by macrophages...
  53. Novel Therapies in Idiopathic Pulmonary Fibrosis
    Kevin Brown; Fiscal Year: 2009
    ..A secondary objective will be to determine if the ability of bronchoalveolar lavage fluid to convert fibroblasts to myofibroblasts can be used as a bioassay to monitor biologic disease activity...
  54. ROLE OF EICOSANOIDS IN OZONE-INDUCED LUNG INJURY
    Michael Madden; Fiscal Year: 1993
    ..Additionally bronchoalveolar lavage fluid of humans exposed in vivo to O3 will be analyzed by HPLC in order to determine if peak 1 is released ..
  55. TH2 CELL INDUCTION AND EFFECTOR FUNCTION IN THE AIRWAY
    Lauren Cohn; Fiscal Year: 1999
    ..The models of CD4 T cell subset induction that are proposed include unique transgenic systems in which to study the role of Th2 cells in the development of AHR and bronchial inflammation. ..
  56. SCCOR in Translational Research in Acute Lung Injury
    Michael Matthay; Fiscal Year: 2007
    ..The proteomics core (Core C) will provide innovative methods for identifying novel proteins in the bronchoalveolar lavage fluid from patients and animals with acute lung injury, both before and after several treatment strategies...
  57. NFI AND ALVEOLAR TYPE II CELL FUNCTION
    CINDY BACHURSKI; Fiscal Year: 1999
    ..TNF levels are increased and surfactant proteins are decreased in the bronchoalveolar lavage fluid of ARDS patients...
  58. Chemokine Dynamics in the HIV-1/SIV Infected Lung
    Denise Kirschner; Fiscal Year: 2006
    ..complex networks of cells, cytokines, chemokines, virus, and other pathogens within interstitium and bronchoalveolar lavage fluid (BALF)...
  59. EOSINOPHIL, EICOSANOID, BIOSYNTHESIS AND METABOLISM
    Robert Murphy; Fiscal Year: 2001
    ..have identified for the first time the formation of 5-oxo-ETE in the lung through its appearance in bronchoalveolar lavage fluid of mice...
  60. Modulation of Airway Inflammation By Surfactant Proteins
    Lee Maddox; Fiscal Year: 2002
    ..b>Bronchoalveolar lavage fluid will be sampled for cytokines and lung tissue will be harvested for RNA extraction and histological ..
  61. Innate Immunity and Environmental Airway Injury
    John Hollingsworth; Fiscal Year: 2003
    ..Airway resistance will be measured by Penh and confirmed with APTI. Bronchoalveolar lavage fluid will be sampled for cellular inflammation, protein concentration, and cytokine profile...
  62. ROLE OF THE ALPHAE INTEGRIN IN IMMUNITY TO TUBERCULOSIS
    Samuel Behar; Fiscal Year: 2004
    ..receiving anti-alphaE mAb or mice genetically deficient in alphaE have decreased cellularity of the bronchoalveolar lavage fluid, lower levels of TH2 cytokines, and less airway hyperresponsiveness...
  63. A proteomics approach to study the mechanisms of Fas-induced lung fibrosis
    Dong Chang; Fiscal Year: 2007
    ..In addition, we will perform proteomic analysis on the bronchoalveolar lavage fluid of the chimeric mice to identify the proteins that are expressed in the airspaces when Fas-activates ..