amniotic fluid

Summary

Summary: Amniotic cavity fluid which is produced by the AMNION and fetal lungs and kidneys.

Top Publications

  1. ncbi Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation
    Pieternella S In 't Anker
    Blood 102:1548-9. 2003
  2. ncbi Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol
    Ming Song Tsai
    Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
    Hum Reprod 19:1450-6. 2004
  3. ncbi Secretory profiles and wound healing effects of human amniotic fluid-derived mesenchymal stem cells
    Byung Sun Yoon
    Laboratory of Cell Function Regulation, College of Life Sciences and Biotechnology, Korea University, Anam Dong, Seongbuk gu, Seoul, Republic of Korea
    Stem Cells Dev 19:887-902. 2010
  4. ncbi Clinical applications of prenatal and postnatal therapy using stem cells retrieved from amniotic fluid
    Sheng Wen S Shaw
    Surgery Unit, Institute of Child Health, University College London, London, UK
    Curr Opin Obstet Gynecol 23:109-16. 2011
  5. ncbi Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: comparison to bone marrow mesenchymal stem cells
    Maria G Roubelakis
    Cell and Gene Therapy Laboratory, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens BRF, Athens, Greece
    Stem Cells Dev 16:931-52. 2007
  6. ncbi Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?
    Andrea Romana Prusa
    Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Hum Reprod 18:1489-93. 2003
  7. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
  8. ncbi In vitro and in vivo properties of distinct populations of amniotic fluid mesenchymal progenitor cells
    Maria G Roubelakis
    Cell and Gene Therapy Laboratory, Centre of Basic Research, Biomedical Research Foundation, Academy of Athens BRFAA, Athens, Greece
    J Cell Mol Med 15:1896-913. 2011
  9. ncbi Therapeutic potential of a distinct population of human amniotic fluid mesenchymal stem cells and their secreted molecules in mice with acute hepatic failure
    Dimitra S Zagoura
    Cell and Gene Therapy Laboratory, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens BRFAA, Athens, Greece
    Gut 61:894-906. 2012
  10. ncbi Amniotic fluid: not just fetal urine anymore
    Mark A Underwood
    Department of Pediatrics, University of California, Davis School of Medicine, Davis, CA 95616, USA
    J Perinatol 25:341-8. 2005

Detail Information

Publications332 found, 100 shown here

  1. ncbi Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation
    Pieternella S In 't Anker
    Blood 102:1548-9. 2003
  2. ncbi Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol
    Ming Song Tsai
    Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
    Hum Reprod 19:1450-6. 2004
    The aim of this study was to isolate mesenchymal stem cells (MSCs) from amniotic fluid obtained by second-trimester amniocentesis.
  3. ncbi Secretory profiles and wound healing effects of human amniotic fluid-derived mesenchymal stem cells
    Byung Sun Yoon
    Laboratory of Cell Function Regulation, College of Life Sciences and Biotechnology, Korea University, Anam Dong, Seongbuk gu, Seoul, Republic of Korea
    Stem Cells Dev 19:887-902. 2010
    Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy...
  4. ncbi Clinical applications of prenatal and postnatal therapy using stem cells retrieved from amniotic fluid
    Sheng Wen S Shaw
    Surgery Unit, Institute of Child Health, University College London, London, UK
    Curr Opin Obstet Gynecol 23:109-16. 2011
    To review the potential of stem cells derived from amniotic fluid and applications in prenatal and postnatal therapy.
  5. ncbi Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: comparison to bone marrow mesenchymal stem cells
    Maria G Roubelakis
    Cell and Gene Therapy Laboratory, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens BRF, Athens, Greece
    Stem Cells Dev 16:931-52. 2007
    ..In the present study, we isolated and expanded fetal MSCs from second-trimester amniotic fluid (AF)...
  6. ncbi Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?
    Andrea Romana Prusa
    Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Wahringer Gurtel 18 20, 1090 Vienna, Austria
    Hum Reprod 18:1489-93. 2003
    ..Oct-4 is a marker for pluripotent human stem cells so far known to be expressed in embryonal carcinoma cells, embryonic stem cells and embryonic germ cells...
  7. ncbi The role of inflammation and infection in preterm birth
    Roberto Romero
    Perinatology Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA
    Semin Reprod Med 25:21-39. 2007
    ..The isolation of bacteria in the amniotic fluid, known as microbial invasion of the amniotic cavity, is a pathological finding; the frequency of which is ..
  8. ncbi In vitro and in vivo properties of distinct populations of amniotic fluid mesenchymal progenitor cells
    Maria G Roubelakis
    Cell and Gene Therapy Laboratory, Centre of Basic Research, Biomedical Research Foundation, Academy of Athens BRFAA, Athens, Greece
    J Cell Mol Med 15:1896-913. 2011
    ..Recently, we identified and characterized human second trimester amniotic fluid (AF) as a novel source of MPCs...
  9. ncbi Therapeutic potential of a distinct population of human amniotic fluid mesenchymal stem cells and their secreted molecules in mice with acute hepatic failure
    Dimitra S Zagoura
    Cell and Gene Therapy Laboratory, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens BRFAA, Athens, Greece
    Gut 61:894-906. 2012
    ..In previous studies, a distinct population termed human spindle-shaped MSCs were isolated and expanded from second trimester amniotic fluid (AF-MSCs) and characterised based on their phenotype, pluripotency and differentiation potential.
  10. ncbi Amniotic fluid: not just fetal urine anymore
    Mark A Underwood
    Department of Pediatrics, University of California, Davis School of Medicine, Davis, CA 95616, USA
    J Perinatol 25:341-8. 2005
    b>Amniotic fluid (AF) is a complex substance essential to fetal well-being. This article reviews recent discoveries and the current understanding of the origin and circulation of AF and its nutritive, protective, and diagnostic functions...
  11. ncbi Functional network analysis of the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow
    Ming Song Tsai
    Prenatal Diagnosis Center, Cathay General Hospital, Taipei, Taiwan
    Stem Cells 25:2511-23. 2007
    ..Our results suggest that amniotic fluid MSCs may initiate interactions with the uterus by upregulating oxytocin and thrombin receptors...
  12. ncbi Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential
    Patrizia Bossolasco
    Fondazione Matarelli, 20121 Milan, Italy
    Cell Res 16:329-36. 2006
    ..b>Amniotic fluid cells (AFCs) are routinely obtained for prenatal diagnosis and can be expanded in vitro; nevertheless current ..
  13. ncbi Amniotic membrane and amniotic fluid-derived cells: potential tools for regenerative medicine?
    Ornella Parolini
    Centro di Ricerca E Menni, Fondazione Poliambulanza Istituto Ospedaliero, Via Bissolati, 57, 25124 Brescia, Italy
    Regen Med 4:275-91. 2009
    Human amniotic membranes and amniotic fluid have attracted increasing attention in recent years as a possible reserve of stem cells that may be useful for clinical application in regenerative medicine...
  14. pmc The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum
    Christine L Knox
    Institute of Health and Biomedical Innovation, and School of Life Sciences, Queensland University of Technology, Brisbane, Queensland, Australia
    Biol Reprod 83:415-26. 2010
    Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid in asymptomatic pregnancies and placental infections. Ureaplasma parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women...
  15. ncbi Renal differentiation of amniotic fluid stem cells
    L Perin
    Childrens Hospital Los Angeles, Saban Research Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA 90027, USA
    Cell Prolif 40:936-48. 2007
    ..the application, for kidney regeneration, of a novel non-genetically modified stem cell, derived from human amniotic fluid. We show that these pluripotent cells can develop and differentiate into de novo kidney structures during ..
  16. ncbi Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells
    J Kim
    Department of Biotechnology, College of Natural Science, Seoul Women s University, Nowon Gu, Seoul, Korea
    Cell Prolif 40:75-90. 2007
    To characterize mesenchymal stem cell-like cells isolated from human amniotic fluid for a new source of therapeutic cells.
  17. ncbi Human and murine amniotic fluid c-Kit+Lin- cells display hematopoietic activity
    Andrea Ditadi
    Inserm U768, Paris, France
    Blood 113:3953-60. 2009
    We have isolated c-Kit(+)Lin(-) cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential...
  18. ncbi Antimicrobial polypeptides of human vernix caseosa and amniotic fluid: implications for newborn innate defense
    Hiroyuki Yoshio
    Department of Woman and Child Health, Karolinska Institutet, Stockholm, Sweden
    Pediatr Res 53:211-6. 2003
    ..of newborns at birth, we have characterized antimicrobial polypeptides in vernix caseosa (vernix) and amniotic fluid (AF)...
  19. ncbi Amniotic fluid derived stem cells ameliorate focal cerebral ischaemia-reperfusion injury induced behavioural deficits in mice
    Ashish K Rehni
    Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, Punjab, India
    Behav Brain Res 183:95-100. 2007
    The present study has been designed to investigate the effect of amniotic fluid derived stem cells on focal cerebral ischaemia-reperfusion injury induced behavioural deficits in mice...
  20. ncbi High transduction efficiency of human amniotic fluid stem cells mediated by adenovirus vectors
    Davide Grisafi
    Gene Therapy Laboratory and Centre for Rare Diseases, Department of Pediatrics, University of Padova, Padova, Italy
    Stem Cells Dev 17:953-62. 2008
    ..last few years some studies have shown the possibility of deriving progenitors with various potential from the amniotic fluid. Amniocentesis is a widely accepted method for prenatal diagnosis; it is associated with low risk both for the ..
  21. pmc Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium
    Misty Good
    Division of Newborn Medicine, Children s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA
    Proc Natl Acad Sci U S A 109:11330-5. 2012
    ..To define endogenous strategies that could reduce TLR4 signaling, we hypothesized that amniotic fluid can inhibit TLR4 signaling within the fetal intestine and attenuate experimental NEC, and we sought to ..
  22. ncbi Human amniotic fluid-derived stem cells expressing cytosine deaminase and thymidine kinase inhibits the growth of breast cancer cells in cellular and xenograft mouse models
    N H Kang
    Laboratory of Veterinary Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea
    Cancer Gene Ther 19:412-9. 2012
    As human amniotic fluid-derived stem cells (hAFSCs) are capable of multiple lineage differentiation, extensive self-renewal and tumor targeting, they may be valuable for clinical anticancer therapies...
  23. ncbi Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix
    Tullia Maraldi
    Department of Laboratories, Pathological Anatomy and Forensic Medicine, University of Modena and Reggio Emilia, Modena, Italy
    Tissue Eng Part A 17:2833-43. 2011
    This study investigated the potential of amniotic fluid stem cells (AFSCs) to synthesize mineralized extracellular matrix (ECM) within different porous scaffolds of collagen, poly-D,L-lactic acid (PDLLA), and silk fibroin...
  24. pmc Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses
    Donna K Slonim
    Department of Computer Science, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 106:9425-9. 2009
    ..syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples...
  25. ncbi Racial disparity in meconium-stained amniotic fluid and meconium aspiration syndrome in the United States, 1989-2000
    Sudhir Sriram
    Neonatology Section and Center for Perinatal Epidemiology, University of Chicago Children s Hospital, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Obstet Gynecol 102:1262-8. 2003
    To estimate the prevalence of meconium-stained amniotic fluid and meconium aspiration syndrome, as well as the differences in case fatality from meconium aspiration syndrome, between non-Hispanic black and non-Hispanic white infants.
  26. ncbi Prediction of microbial invasion of the amniotic cavity in women with preterm labour: analysis of multiple proteins in amniotic and cervical fluids
    R M Holst
    Perinatal Centre, Department of Obstetrics and Gynaecology, Institute for Clinical Sciences, Sahlgrenska University Hospital East, Goteborg, Sweden
    BJOG 118:240-9. 2011
    ..The objective was to determine whether specific proteins in amniotic and cervical fluids alone, or in combination, could identify bacterial invasion...
  27. ncbi The bacterial load of Ureaplasma parvum in amniotic fluid is correlated with an increased intrauterine inflammatory response
    David C Kasper
    Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, A 1090 Vienna, Austria
    Diagn Microbiol Infect Dis 67:117-21. 2010
    ..We analyzed 118 samples from amniotic fluid of preterm infants before 34 weeks of gestation by quantitative polymerase chain reaction (qPCR)...
  28. ncbi The potential of amniotic fluid stem cells for cellular therapy and tissue engineering
    Petra A B Klemmt
    Georg Speyer Haus, Institute for Biomedical Research, Paul Ehrlich Str 42 44, 60596 Frankfurt, Germany
    Expert Opin Biol Ther 11:1297-314. 2011
    Foetal cells present in amniotic fluid (AF) have been used for many years to perform prenatal genetic screening. Recent reports suggested that these cells might have additional benefits...
  29. ncbi Multiplex ligation-dependent probe amplification (MLPA) as a stand-alone test for rapid aneuploidy detection in amniotic fluid cells
    Angelique J A Kooper
    Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Prenat Diagn 28:1004-10. 2008
    ..e. 13, 18, 21, X and Y) in amniotic fluid cells in routine prenatal clinical practice.
  30. pmc Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats
    Naoki Tajiri
    Department of Neurosurgery and Brain Repair, Center of Excellence for Aging and Brain Repair, University of South Florida Morsani College of Medicine, Tampa, Florida, United States of America
    PLoS ONE 7:e43779. 2012
    We recently reported isolation of viable rat amniotic fluid-derived stem (AFS) cells [1]. Here, we tested the therapeutic benefits of AFS cells in a rodent model of ischemic stroke...
  31. ncbi Probing stemness and neural commitment in human amniotic fluid cells
    Anna Jezierski
    Neurogenesis and Brain Repair, Neurobiology Program, Institute for Biological Sciences, National Research Council Canada, 1200 Montreal Road, Ottawa, Canada
    Stem Cell Rev 6:199-214. 2010
    Recently, human amniotic fluid (AF) cells have attracted a great deal of attention as an alternative cell source for transplantation and tissue engineering...
  32. pmc Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth
    Yiping W Han
    Department of Periodontics, School of Dental Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4905, USA
    Infect Immun 72:2272-9. 2004
    ..It is also associated with preterm birth and has been isolated from the amniotic fluid, placenta, and chorioamnionic membranes of women delivering prematurely...
  33. pmc Valproic acid confers functional pluripotency to human amniotic fluid stem cells in a transgene-free approach
    Dafni Moschidou
    Imperial College London, London, UK
    Mol Ther 20:1953-67. 2012
    ..Here, we show that c-KIT+ human first-trimester amniotic fluid stem cells (AFSCs) can be fully reprogrammed to pluripotency without ectopic factors, by culture on Matrigel ..
  34. ncbi Selection of alkaline phosphatase-positive induced pluripotent stem cells from human amniotic fluid-derived cells by feeder-free system
    Huai En Lu
    Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC
    Exp Cell Res 317:1895-903. 2011
    ..In this study, we co-expressed through lenti-viral delivery the Yamanaka factors in amniotic fluid-derived (AF) cells...
  35. ncbi Early oxidative stress in amniotic fluid of pregnancies with Down syndrome
    S Perrone
    Department of Pediatrics, Obstetrics and Reproductive Medicine, University of Siena, Policlinico Le Scotte, V le Bracci 36, 53100 Siena, Italy
    Clin Biochem 40:177-80. 2007
    ..Some evidence suggests that oxidative stress, due to an imbalance between oxidants and antioxidants, occurs in babies with Down syndrome (DS). This study tests the hypothesis that oxidative stress occurs early in DS pregnancies...
  36. ncbi Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem-cell differentiation
    Christiane Fuchs
    Institute of Medical Genetics, Medical University of Vienna, Währinger Strasse 10, Vienna 1090, Austria
    Hum Mol Genet 21:1049-61. 2012
    ..Human amniotic fluid stem (AFS) cells harbor high proliferation potential, do not raise the ethical issues of embryonic stem cells, ..
  37. pmc Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes
    Daniel B Digiulio
    Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA
    Am J Reprod Immunol 64:38-57. 2010
    ..The role played by microbial invasion of the amniotic cavity (MIAC) in preterm pre-labor rupture of membranes (pPROM) is inadequately characterized, in part because of reliance on cultivation-based methods...
  38. ncbi Contribution of human amniotic fluid stem cells to renal tissue formation depends on mTOR
    Nicol Siegel
    Institute of Medical Genetics, Medical University of Vienna, Währinger Strasse 10, 1090 Vienna, Austria
    Hum Mol Genet 19:3320-31. 2010
    Human amniotic fluid stem cells (hAFSCs) can be grown in large quantities, have a low risk for tumour development and harbour a high differentiation potential...
  39. ncbi Isolation of amniotic stem cell lines with potential for therapy
    Paolo De Coppi
    Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1094, USA
    Nat Biotechnol 25:100-6. 2007
    ..We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers...
  40. ncbi Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells
    Chunliang Li
    Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Shanghai JiaoTong University School of Medicine, Shanghai, China
    Hum Mol Genet 18:4340-9. 2009
    ..Here, we report for the first time rapid and efficient generation of iPS cells from human amniotic fluid-derived cells (hAFDCs) via ectopic expression of four human factors: OCT4/SOX2/KLF4/C-MYC...
  41. ncbi Association between prenatal exposure to phthalates and the health of newborns
    Po Chin Huang
    Department of Environmental and Occupational Health, Medical College, National Cheng Kung University, Tainan, Taiwan
    Environ Int 35:14-20. 2009
    ..were to evaluate the association between maternal urine excretion, the exposure of fetus to phthalates in amniotic fluid, and the health of newborns...
  42. ncbi Chondrogenic differentiation of amniotic fluid-derived stem cells
    Yash M Kolambkar
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
    J Mol Histol 38:405-13. 2007
    ..b>Amniotic fluid-derived stem (AFS) cells have recently been isolated from human and rodent amniotic fluid and shown to be ..
  43. ncbi Diagnosis of intra-amniotic infection by proteomic profiling and identification of novel biomarkers
    Michael G Gravett
    Division of Reproductive Sciences, Oregon National Primate Research Center, Beaverton, Ore, USA
    JAMA 292:462-9. 2004
    ..Intra-amniotic infection (IAI) is commonly associated with preterm birth and adverse neonatal sequelae. Early diagnosis of IAI, however, has been hindered by insensitive or nonspecific tests...
  44. pmc Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery
    Peter V Hauser
    Renal and Vascular Physiopathology Laboratory, Department of Internal Medicine, Molecular Biotechnology Centre, University of Torino, Torino, Italy
    Am J Pathol 177:2011-21. 2010
    Stem cells isolated from human amniotic fluid are gaining attention with regard to their therapeutic potential. In this work, we investigated whether these cells contribute to tubular regeneration after experimental acute kidney injury...
  45. ncbi The amniotic fluid as a source of cells for fetal tissue engineering
    A Kaviani
    Children's Hospital, 300 Longwood Ave, Fegan 3, Boston, MA 02115, USA
    J Pediatr Surg 36:1662-5. 2001
    ..was aimed at determining whether fetal tissue constructs can be engineered from cells normally found in the amniotic fluid. METHODS: A subpopulation of morphologically distinct cells was isolated mechanically from the amniotic fluid ..
  46. ncbi Characterization and hepatogenic differentiation of mesenchymal stem cells from human amniotic fluid and human bone marrow: a comparative study
    Yu Bao Zheng
    Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat Sen University, Guangzhou 510630, Guangdong Province, PR China
    Cell Biol Int 32:1439-48. 2008
    ..was to investigate the in vitro biological characterization and hepatic differentiation potential of human amniotic fluid-derived mesenchymal stem cells (AF-hMSCs) and human bone marrow-derived mesenchymal stem cells (BM-hMSCs)...
  47. ncbi Amniotic fluid cells and human stem cell research: a new connection
    Andrea Romana Prusa
    Department of Prenatal Diagnosis and Therapy, Obstetrics and Gynecology, University of Vienna, Vienna, Austria
    Med Sci Monit 8:RA253-7. 2002
    ..Despite the widespread and well-established use of amniotic fluid cells in routine prenatal genetic testing, current knowledge about the origin and properties of these cells is ..
  48. pmc Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes
    Joseph R Biggio
    Department of Obstetrics and Gynecology, University of Alabama at Birmingham, USA
    Am J Obstet Gynecol 192:109-13. 2005
    We sought to determine whether midtrimester amniotic fluid levels of matrix metalloproteinase-8 were associated with subsequent preterm premature rupture of membranes.
  49. ncbi Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide
    Ji Min Seo
    Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea
    Cryobiology 62:167-73. 2011
    b>Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use...
  50. ncbi Choriodecidual infection and preterm birth
    Robert L Goldenberg
    University of Alabama at Birmingham, Department of Obstetrics and Gynecology, 35249 7333, USA
    Nutr Rev 60:S19-25. 2002
    ..Results of treatment trials to eradicate the infection and reduce preterm birth have been mixed...
  51. ncbi Amniotic fluid stem cells are cardioprotective following acute myocardial infarction
    Sveva Bollini
    Surgery Unit, Institute of Child Health and Great Ormond Street Hospital, University College London, London, UK
    Stem Cells Dev 20:1985-94. 2011
    ..We have previously described the isolation of broadly multipotent cells from amniotic fluid, defined as amniotic fluid stem (AFS) cells...
  52. ncbi The heterogeneity of cell subtypes from a primary culture of human amniotic fluid
    Shengli Zhang
    Department of Urology, Shanghai Children s Hospital, Shanghai Jiao Tong University School of Medicine, China
    Cell Mol Biol Lett 15:424-39. 2010
    Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture...
  53. ncbi Antimicrobial peptides in amniotic fluid: defensins, calprotectin and bacterial/permeability-increasing protein in patients with microbial invasion of the amniotic cavity, intra-amniotic inflammation, preterm labor and premature rupture of membranes
    J Espinoza
    Perinatology Research Branch, National Institute of Child Health and Human Development NIH DHSS, Bethesda, Maryland, USA
    J Matern Fetal Neonatal Med 13:2-21. 2003
    ..this study was to determine whether parturition, premature rupture of the membranes (PROM) and microbial invasion of the amniotic cavity (MIAC) are associated with changes in amniotic fluid concentrations of these antimicrobial peptides.
  54. ncbi Soluble TLR2 is present in human amniotic fluid and modulates the intraamniotic inflammatory response to infection
    Antonette T Dulay
    Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA
    J Immunol 182:7244-53. 2009
    ..functional role of sTLR2 in modulating the intraamniotic inflammatory response to infection, we studied 109 amniotic fluid samples of women with normal pregnancy outcomes (n = 28) and women with (n = 39) and without (n = 42) ..
  55. ncbi Amniotic fluid interleukin-6 and tumor necrosis factor-alpha at mid-trimester genetic amniocentesis: relationship to intra-amniotic microbial invasion and preterm delivery
    Nikolaos Thomakos
    1st Department of Obstetrics and Gynecology, Alexandra Maternity Hospital, Athens University, Athens, Greece
    Eur J Obstet Gynecol Reprod Biol 148:147-51. 2010
    To determine the value of amniotic fluid interleukin-6 (AF IL-6) and tumor necrosis factor-alpha (AF TNF-alpha) in the diagnosis of microbial invasion of the amniotic cavity and in the prediction of preterm delivery (PTD).
  56. ncbi Microarray comparative genomic hybridization (CGH)-based prenatal diagnosis for chromosome abnormalities using cell-free fetal DNA in amniotic fluid
    Shoko Miura
    Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1 7 1, 852 8501, Nagasaki, Japan
    J Hum Genet 51:412-7. 2006
    Cell-free fetal DNA (cffDNA) in the supernatant of amniotic fluid, which is usually discarded, can be used as a sample for prenatal diagnosis...
  57. pmc The involvement of human amnion in histologic chorioamnionitis is an indicator that a fetal and an intra-amniotic inflammatory response is more likely and severe: clinical implications
    Chan Wook Park
    Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 28 Yongon Dong, Chongno Gu, Seoul 110 744, Republic of Korea
    Placenta 30:56-61. 2009
    ....
  58. pmc Amniotic fluid stem cells produce robust mineral deposits on biodegradable scaffolds
    Alexandra Peister
    Morehouse College, Department of Biology, Atlanta, Georgia 30317, USA
    Tissue Eng Part A 15:3129-38. 2009
    ..This study investigated the potential of amniotic fluid-derived stem (AFS) cells to synthesize mineralized extracellular matrix within porous medical-grade poly-..
  59. ncbi Amniotic fluid stem cells: a promising therapeutic resource for cell-based regenerative therapy
    Ivana Antonucci
    Department of Oral Sciences, Nano and Biotechnologies, G D Annunzio University, Chieti, Italy
    Curr Pharm Des 18:1846-63. 2012
    ..b>Amniotic fluid -derived Stem cells (AFS) are considered a novel resource for cell transplantation therapy, due to their high ..
  60. ncbi Amniotic fluid chemokines and autism spectrum disorders: an exploratory study utilizing a Danish Historic Birth Cohort
    Morsi W Abdallah
    Department of Epidemiology, Aarhus University School of Public Health, Aarhus, Denmark
    Brain Behav Immun 26:170-6. 2012
    ..The aim of this study was to examine chemokine levels in amniotic fluid (AF) samples of individuals diagnosed with ASD and their controls.
  61. ncbi Intraamniotic infection with genital mycoplasmas exhibits a more intense inflammatory response than intraamniotic infection with other microorganisms in patients with preterm premature rupture of membranes
    Kyung Joon Oh
    Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea
    Am J Obstet Gynecol 203:211.e1-8. 2010
    ..The objective of the study was to compare the intensity of inflammatory responses between intraamniotic infection with genital mycoplasmas and intraamniotic infection with other microorganisms...
  62. pmc In vitro cardiomyogenic potential of human amniotic fluid stem cells
    Xuan Guan
    Wake Forest Institute for Regenerative Medicine, Wake Forest University of Health Sciences, Winston Salem, NC 27157, USA
    J Tissue Eng Regen Med 5:220-8. 2011
    ..In this study, we investigated whether human amniotic fluid-derived stem (hAFS) cells could be a potential source of cells for cardiac cell therapy, by testing the in ..
  63. ncbi Amniotic fluid as a rich source of mesenchymal stromal cells for transplantation therapy
    Ivana Antonucci
    Chieti University and Stem TeCh Group, Aging Research Center CeSI, Chieti, Italy
    Cell Transplant 20:789-95. 2011
    Stem cells isolated from amniotic fluid are known to be able to differentiate into different cells types, thus being considered as a powerful tool for cellular therapy of different human diseases...
  64. pmc Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues
    Hassan Abdulrazzak
    Institute of Reproductive and Developmental Biology, Imperial College London, London W12 0NN, UK
    J R Soc Interface 7:S689-706. 2010
    ..tissues such as blood, liver and bone marrow as well as from a variety of extraembryonic tissues such as amniotic fluid and placenta...
  65. ncbi Comparative analysis of the retinal potential of embryonic stem cells and amniotic fluid-derived stem cells
    Sarah Decembrini
    UCL Institute of Child Health, Great Ormond Street Hospital, London, United Kingdom
    Stem Cells Dev 20:851-63. 2011
    ..Human and mouse amniotic fluid-derived stem cells (AFCs) have been reported to be multipotent and express embryonic and adult stem cell ..
  66. pmc Experimental amniotic fluid infection in sheep: effects of Ureaplasma parvum serovars 3 and 6 on preterm or term fetal sheep
    Timothy J M Moss
    School of Women s and Infants Health, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Perth, Western Australia, Australia
    Am J Obstet Gynecol 198:122.e1-8. 2008
    ..The objective of the study was to determine the effects in late gestation of Ureaplasma parvum serovar 3 colonization and the effects, preterm, of U. parvum serovar 6...
  67. ncbi Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes
    Soon Sup Shim
    Department of Obstetrics and Gynecology, Laboratory of Fetal Medicine Research, College of Medicine, Seoul National University Seoul 110 744, Korea
    Am J Obstet Gynecol 191:1339-45. 2004
    ..This study was conducted to determine the frequency and clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of the membranes...
  68. ncbi Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery
    Chan Kyung J Cho
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    J Proteome Res 9:3574-82. 2010
    ..Since amniotic fluid (AF) surrounds the developing fetus, understanding the changes in AF composition in the presence of DS may ..
  69. ncbi Neurogenic differentiation of amniotic fluid stem cells
    M Rosner
    Institute of Medical Genetics, Medical University of Vienna, Währinger Strasse 10, 1090, Vienna, Austria
    Amino Acids 42:1591-6. 2012
    In 2003, human amniotic fluid has been shown to contain stem cells expressing Oct-4, a marker for pluripotency. This finding initiated a rapidly growing and very promising new stem cell research field...
  70. ncbi Neurogenic cells in human amniotic fluid
    Andrea Romana Prusa
    Department of Obstetrics and Gynecology, University of Vienna, Prenatal Diagnosis and Therapy, Vienna, Austria
    Am J Obstet Gynecol 191:309-14. 2004
    The purpose of this study was to determine whether human amniotic fluid contains cells that harbor the potential to differentiate into neurogenic cells.
  71. ncbi Maternally administered melatonin differentially regulates lipopolysaccharide-induced proinflammatory and anti-inflammatory cytokines in maternal serum, amniotic fluid, fetal liver, and fetal brain
    De Xiang Xu
    Department of Toxicology, Anhui Medical University, Hefei, China
    J Pineal Res 43:74-9. 2007
    ..administered melatonin on LPS-induced proinflammatory and anti-inflammatory cytokines in maternal serum, amniotic fluid, fetal liver and fetal brain. The time pregnant mice were injected with melatonin [5...
  72. ncbi Racial disparity in amniotic fluid concentrations of tumor necrosis factor (TNF)-alpha and soluble TNF receptors in spontaneous preterm birth
    Ramkumar Menon
    Perinatal Research Center, Nashville, TN 37203, USA
    Am J Obstet Gynecol 198:533.e1-10. 2008
    ..The objective of this study is to examine the inflammatory cytokine, tumor necrosis factor (TNF)-alpha and soluble TNF receptor concentrations (sTNFR1 and sTNFR2) in the amniotic fluid of black and white women at delivery.
  73. ncbi Endotoxin-neutralizing antimicrobial proteins of the human placenta
    Hun Sik Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, Taejon 305 701, Korea
    J Immunol 168:2356-64. 2002
    ..to the constitutive release of histone H2B, and histones H2A and H2B contribute to bactericidal activity of amniotic fluid. Our studies suggest that histones H2A and H2B may endow the epithelium of the placenta with an antimicrobial ..
  74. ncbi Purification and antimicrobial activity studies of the N-terminal fragment of ubiquitin from human amniotic fluid
    Jin Young Kim
    Research Center for Proteineous Materials, Chosun University, Kwangju 501 759, Korea
    Biochim Biophys Acta 1774:1221-6. 2007
    A 4.3-kDa antimicrobial peptide was isolated from human amniotic fluid by dialysis, ultrafiltration, and C18 reversed-phase high performance liquid chromatography...
  75. ncbi Amniotic fluid stem cells increase embryo survival following injury
    Weerapong Prasongchean
    Developmental Biology Unit, UCL Institute of Child Health, London, UK
    Stem Cells Dev 21:675-88. 2012
    Although amniotic fluid cells can differentiate into several mesenchymal lineages and have been proposed as a valuable therapeutic cell source, their ability to undergo terminal neuronal differentiation remains a cause of controversy...
  76. ncbi hCG in trophoblast tumour cells of the cell line Jeg3 and hCG isolated from amniotic fluid and serum of pregnant women carry oligosaccharides of the sialyl Lewis X and sialyl Lewis a type
    Udo Jeschke
    University of Rostock, Department of Obstetrics and Gynaecology, Faculty of Medicine, Doberaner Str 142, D 18055 Rostock, Germany
    Anticancer Res 23:1087-92. 2003
    ..For this study hCG was purified from pooled human mid-trimester amniotic fluid, serum and urine of pregnant women and from supernatants of trophoblast tumour cell cultures Jeg3 and BeWo by ..
  77. ncbi Monocyte chemotactic protein-1 is increased in the amniotic fluid of women who deliver preterm in the presence or absence of intra-amniotic infection
    M Sean Esplin
    Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, Utah, USA
    J Matern Fetal Neonatal Med 17:365-73. 2005
    ..This study was conducted to determine if preterm labor leading to preterm delivery and intra-amniotic infection (IAI) are associated with changes in the amniotic fluid concentrations of MCP-1.
  78. ncbi BMP15 gene is activated during human amniotic fluid stem cell differentiation into oocyte-like cells
    Xiang Cheng
    Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A and F University, Yangling, Shaanxi, China
    DNA Cell Biol 31:1198-204. 2012
    ..investigate the activation of bone morphogenetic protein 15 gene (BMP15) during the differentiation of human amniotic fluid stem cells (hAFSCs) into OLCs...
  79. ncbi Oxidative stress occurs early in Down syndrome pregnancy: A redox proteomics analysis of amniotic fluid
    Marzia Perluigi
    Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
    Proteomics Clin Appl 5:167-78. 2011
    The present study aims to evaluate a set of oxidative stress biomarkers in the amniotic fluid (AF) of women carrying Down syndrome (DS) fetuses that could prove in vivo the early occurrence of oxidative damage in DS.
  80. ncbi Amniotic fluid interleukin-6 and preterm delivery: a review
    A Y El-Bastawissi
    Center for Perinatal Studies, Swedish Medical Center, Seattle, Washington, USA
    Obstet Gynecol 95:1056-64. 2000
    To evaluate the potential role of amniotic fluid (AF) interleukin (IL)-6 as a predictor of preterm delivery and to consider possible explanations for the proportion of women with elevated AF IL-6 who deliver preterm yet lack ..
  81. pmc Measurement of pesticides and other toxicants in amniotic fluid as a potential biomarker of prenatal exposure: a validation study
    Asa Bradman
    Center for Children s Environmental Health Research, School of Public Health, University of California, Berkeley, California 94720 7380, USA
    Environ Health Perspect 111:1779-82. 2003
    ..No studies have been published that report measurements of commonly used nonpersistent pesticides in human amniotic fluid, although recent studies of pesticides in urine from pregnant women and in meconium indicate that fetuses are ..
  82. pmc The LARGE principle of cellular reprogramming: lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells
    Katharina Wolfrum
    Molecular Embryology and Aging Group, Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin, Germany
    PLoS ONE 5:e13703. 2010
    Human amniotic fluid cells (AFCs) are routinely obtained for prenatal diagnostics procedures...
  83. ncbi Proteins in leaked amniotic fluid as biomarkers diagnostic for prelabor rupture of membranes
    Tao Wang
    Laboratory of Molecular and Translational Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, P R China
    Proteomics Clin Appl 5:415-21. 2011
    ..Early diagnosis of prelabor rupture of membranes (PROM) is essential to protect mother and fetus from intra-uterus infection and preterm birth. A simple and rapid bedside test would help clinicians confirm the diagnosis for early treatment...
  84. ncbi MMP/TIMP imbalance in amniotic fluid during PROM: an indirect support for endogenous pathway to membrane rupture
    S J Fortunato
    Perinatal Research Center, Women s Health Research and Education Foundation, Nashville, TN, USA
    J Perinat Med 27:362-8. 1999
    ..This study examines the levels of the gelatinases and their natural inhibitors (tissue inhibitor of matrix metalloproteinases-TIMPs) in the amniotic fluid during PROM, preterm labor (PTL) and at term.
  85. ncbi Potential role of culture mediums for successful isolation and neuronal differentiation of amniotic fluid stem cells
    M Orciani
    Department of Molecular Pathology and Innovative Therapies, Histology Section, Marche Polytechnic University, Ancona, Italy
    Int J Immunopathol Pharmacol 21:595-602. 2008
    ..Recently, a number of reports suggests that another suitable source of multipotent stem cells may be the amniotic fluid. Amniotic fluid mesenchymal stem cells (AFMSCs) are capable of extensive self-renewal, able to differentiate ..
  86. pmc Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis
    Laura Perin
    Developmental Biology and Regenerative Medicine Program, Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 5:e9357. 2010
    ..We have previously shown that clonal c-kit(pos) stem cells, derived from human amniotic fluid (hAFSC) can be induced to a renal fate in an ex-vivo system...
  87. ncbi Proteomic analysis of amniotic fluid in pregnancies with Klinefelter syndrome foetuses
    Athanasios K Anagnostopoulos
    Medical Genetics, Athens University School of Medicine, Athens, Greece
    J Proteomics 73:943-50. 2010
    ..In the present study proteomic analysis was performed in twelve 2nd trimester amniotic fluid samples, eight coming from pregnancies with normal males and four with Klinefelter syndrome foetuses, as shown ..
  88. ncbi Amniotic fluid insulin at 14-20 weeks' gestation: association with later maternal glucose intolerance and birth macrosomia
    M W Carpenter
    Department of Obstetrics and Gynecology, Brown University School of Medicine, Providence, Rhode Island, USA
    Diabetes Care 24:1259-63. 2001
    To examine the hypothesis that early second trimester amniotic fluid (AF) insulin concentration is elevated and later fetal growth is augmented in gravidas demonstrating later oral glucose intolerance.
  89. ncbi Fetal nucleic acids in maternal body fluids: an update
    Diana W Bianchi
    Tufts New England Medical Center, Box 394, 750 Washington Street, Boston, MA 02111, USA
    Ann N Y Acad Sci 1075:63-73. 2006
    ..to be actively involved in the development of new biomarkers for prenatal diagnosis using maternal blood and amniotic fluid. We have also developed a mouse model that demonstrates that cell-free fetal (cff) DNA is detectable in the ..
  90. ncbi Evaluation of amniotic fluid cytokines in preterm labor and intact membranes
    Reinaldo Figueroa
    Department of Obstetrics, Cell Biology and Anatomy, New York Medical College, Valhalla, NY 11501, USA
    J Matern Fetal Neonatal Med 18:241-7. 2005
    To compare the amniotic fluid (AF) concentration of pro-inflammatory cytokines between women with preterm labor and intact membranes that delivered within 7 days, with those that delivered after 7 days of the amniocentesis according to ..
  91. ncbi Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat
    Angela Chiavegato
    Deparment of Biomedical Sciences, University of Padua, Viale G Colombo, 3 35121 Padova, Italy
    J Mol Cell Cardiol 42:746-59. 2007
    Human amniotic fluid-derived stem (AFS) cells, similarly to embryonic stem cells, could possess privileged immunological characteristics suitable for a successful transplantation even in a discordant xenograft system...
  92. ncbi Elevated second trimester amniotic fluid interferon gamma-inducible T-cell alpha chemoattractant concentrations as a possible predictor of preterm birth
    Ariadne Malamitsi-Puchner
    University of Athens, Medical School, Athens, Greece
    J Soc Gynecol Investig 13:25-9. 2006
    To determine and correlate midtrimester amniotic fluid concentrations of interferon gamma-inducible T-cell alpha chemoattractant (ITAC, a chemokine directing the migration of activated T lymphocytes toward inflammation sites) and C-..
  93. ncbi Proteomic analysis of amniotic fluid in pregnancies with Down syndrome
    George Th Tsangaris
    Division of Biotechnology, Centre of Basic Research, Foundation for Biomedical Research of the Academy of Athens, Athens, Greece
    Proteomics 6:4410-9. 2006
    Proteomic analysis is widely used for the detection of diagnostic markers. In the present study amniotic fluid supernatants (AFS) from pregnancies with Down syndrome (DS) fetuses and from chromosomally normal fetuses in the 17th week of ..
  94. ncbi Relationships between sex hormones assessed in amniotic fluid, and maternal and umbilical cord serum: what is the best source of information to investigate the effects of fetal hormonal exposure?
    Cornelieke van de Beek
    Department of Child and Adolescent Psychiatry, University Medical Centre Utrecht, and the Rudolf Magnus Institute for Neurosciences, Utrecht, The Netherlands
    Horm Behav 46:663-9. 2004
    ..Samples were obtained from amniotic fluid, 2nd and 3rd trimester maternal serum, and umbilical cord serum at birth...
  95. ncbi In vitro differentiation of human amniotic fluid-derived cells: augmentation towards a neuronal dopaminergic phenotype
    Shona Pfeiffer
    University College Dublin, School of Biomolecular and Biomedical Research, Laboratory of Developmental Neurobiology, Conway Institute, Belfield, Dublin 4, Ireland
    Cell Biol Int 34:959-67. 2010
    b>Amniotic fluid is known to yield a number of cell types which are multipotent, ethically derived, genetically stable, easily grown, expanded and possess favourable immunogenicity, which has resulted in an increasing interest for use in ..
  96. ncbi Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells
    Paolo De Coppi
    Department of Pediatrics Pediatric Oncoheamatology, University of Padua, Padua, Italy
    J Urol 177:369-76. 2007
    ..The repair of bladder injury using adult-type stem cells would be useful for adult urological patients but unsuited for neonatal patients, in whom major benefits are likely to derive from fetal-type stem cells...
  97. ncbi Amniotic fluid and cord plasma erythropoietin levels in pregnancies complicated by preeclampsia, pregnancy-induced hypertension and chronic hypertension
    Kari A Teramo
    Department of Obstetrics and Gynecology, University Central Hospital, Helsinki, Finland
    J Perinat Med 32:240-7. 2004
    The purpose of the present study was to compare fetal and neonatal outcomes with amniotic fluid erythropoietin (EPO) levels obtained in the antepartum period in pregnancies complicated by preeclampsia, pregnancy-induced hypertension or ..
  98. ncbi Lysosomal storage diseases in non-immune hydrops fetalis pregnancies
    Angelique J A Kooper
    Department of Human Genetics, Division Cytogenetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Clin Chim Acta 371:176-82. 2006
    ..At least 20 inborn errors of metabolism may cause hydrops fetalis. Most of these are lysosomal storage diseases. The study proposes a diagnostic flowchart for prenatal diagnosis of non-immune hydrops fetalis...
  99. ncbi Midtrimester amniotic fluid leptin and insulin levels and subsequent gestational diabetes
    Rosario D'Anna
    Department of Obstetrics and Gynecology, University of Messina, Messina, Italy
    Gynecol Obstet Invest 64:65-8. 2007
    To evaluate midtrimester amniotic fluid leptin levels in pregnancies subsequently complicated by gestational diabetes.
  100. ncbi Intramembranous absorption rate is unaffected by changes in amniotic fluid composition
    D Anderson
    Department of Physiology and Pharmacology, Oregon Health and Sciences University, Portland, Oregon 97291, USA
    Am J Physiol Renal Physiol 288:F964-8. 2005
    Experiments were performed to determine the effect of amniotic fluid dilution on the rate of intramembranous absorption...
  101. ncbi Neurogenic potential of mesenchymal-like stem cells from human amniotic fluid: the influence of extracellular growth factors
    M Orciani
    Department of Molecular Pathology and Innovative Therapies Histology, Polytechnic University of Marche, Ancona, Italy
    J Biol Regul Homeost Agents 25:115-30. 2011
    ..Immunophenotypical and molecular characterization showed that the 31 amniotic fluid-derived cultures expressed mesenchymal markers as well as some stemness properties...

Research Grants71

  1. Flame Retardant and Adverse Perinatal Outcome (FRAPO)
    Morgan R Peltier; Fiscal Year: 2013
    ..They have also been isolated from the placenta, umbilical chord, amniotic fluid and fetal membranes but their effects on pregnancy outcome are unclear...
  2. Feto-maternal DNA/RNA Trafficking: Biology and Application
    Diana W Bianchi; Fiscal Year: 2013
    ..has expanded exponentially, encompassing evaluation of fetal and placental DNA and mRNA in maternal blood, amniotic fluid, cerebrospinal fluid, and urine...
  3. Human Exposure to Bisphenol A, Phthalates and Fertility, Pregnancy Outcomes
    Russ B Hauser; Fiscal Year: 2013
    ..In addition, ovarian follicular fluid and amniotic fluid samples are collected to assess exposure in relevant target organs or in the fetal compartment, respectively...
  4. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): A normal volume of amniotic fluid (AF) is essential for normal fetal development with favorable perinatal outcome...
  5. Identification of Novel Genes for Congenital Diaphragmatic Hernia
    Wendy K Chung; Fiscal Year: 2012
    ..will accomplish this through the following specific aims: 1) Obtain diaphragm, skin, blood, and when possible amniotic fluid samples from 250 neonates with CDH, 100 children with previously repaired CDH, and amniotic fluid on 250 cases ..
  6. Cesarean Section Optimal Antibiotic Prophylaxis (C/SOAP) Trial
    Alan Thevenet N Tita; Fiscal Year: 2013
    ..Specific aim 3: To collect and store biological specimens (umbilical cord blood and amniotic fluid) for future studies, primarily to assess the association between prenatal exposure to ureaplasmas and adverse ..
  7. 3D human lung model to study lung disease and formation of fibrosis
    Joan E Nichols; Fiscal Year: 2013
    ..Primary and progenitor cells such as HUVEC (primary cells), human amniotic fluid cells, and somatic lung progenitor cells may be used in a supporting role or to populate the matrix...
  8. Duration of Intrapartum Antibiotic Prophylaxis for Group B Streptococcus
    Jessica L Illuzzi; Fiscal Year: 2010
    ..have been found to rapidly reach and maintain minimum inhibitory concentrations in the fetal bloodstream and amniotic fluid. Current protocols from the US Centers for Disease Control and Prevention recommend that intrapartum ..
  9. Diversity &racial disparity in fetal membrane cytokine signature during infectio
    Ramkumar Menon; Fiscal Year: 2012
    ..Racial differences in biomarker concentrations are expected to contribute to racial disparity. Furthermore, amniotic fluid consists of multiple antimicrobial factors and the concentration and regulatory mechanisms of these factors ..
  10. The Effects of Ethanol on Amniotic Fluid-derived Stem Cells
    JENNIFER HIPP; Fiscal Year: 2010
    ..For the current proposal, we plan to use a particular type of stem cells, amniotic fluid-derived stem cells (AFSC), as a model system...
  11. Human Amnion Cell Model for Investigation of Amniotic Fluid Volume
    Cecilia Y Cheung; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Abnormalities in amniotic fluid (AF) volume occur in 5-10% of pregnancies in the US and are often associated with increased maternal and fetal morbidity that compromises pregnancy outcome with ..
  12. Maternal temperament, stress, and inflammation in preterm birth
    LOUIS JOSEPH MUGLIA; Fiscal Year: 2013
    ..stressors plays a key role in the induction of preterm birth;and (3) maternal peripheral blood or amniotic fluid hormones and inflammatory responses will differ prior to and following IL-1 [unreadable] administration during ..
  13. Co-Operative Multicenter Trials in Obstetrics &Maternal Fetal Medicine
    Yoram Sorokin; Fiscal Year: 2013
    ..mid-trimester genetic amniocentesis for clinical indications, and have Ureaplasma urealyticum detected in amniotic fluid, by polymerase chain reaction (PCR)...
  14. REGIONAL DIFFERENTIATION DURING FOREBRAIN DEVELOPMENT
    Anthony S LaMantia; Fiscal Year: 2010
    ..source of signaling molecules available to the forebrain during early development: soluble proteins in the amniotic fluid (AF) when the anterior neural tube is open, and cerebrospinal fluid (CSF) once the anterior neural tube has ..
  15. ASC101 Enhancement of Regenerative Capacity of Amniotic Fluid-derived Stem Cells
    Shay Soker; Fiscal Year: 2012
    ..We will examine amniotic fluid-derived (AFS) stem cells in combination with ASC101 in this small animal CS model...
  16. Development of Pluripotent Amniotic Stem Cell Banks for Disease Modeling
    JAMES FREDERICK ELIASON; Fiscal Year: 2011
    ..Human amniotic fluid (AF) cells have been used for prenatal diagnosis of chromosomal and Mendelian disorders for decades...
  17. Primate model of mid-gestation Ureaplasma in utero infection: Prevention of neuro
    PETA LOUISE GRIGSBY; Fiscal Year: 2013
    ..Biochemical markers characteristic of the fetal inflammatory response, i.e., amniotic fluid levels of PGE2, PGF2[unreadable], pro-inflammatory cytokines (IL-6, TNF-[unreadable], IL-1[unreadable]), total ..
  18. Immunotoxicology of Chronic Exposure to Estrogenic Bisphenol-A
    Norbert E Kaminski; Fiscal Year: 2013
    ..BPA has also been detected in human breast milk, amniotic fluid and cord blood...
  19. Engineered Delivery of Adult Versus Fetal Stem Cells for Bone Regeneration
    Robert E Guldberg; Fiscal Year: 2010
    ..customized gene array analysis to elucidate differential gene regulation pathways of stem cells derived from amniotic fluid and adult bone marrow at baseline and during osteogenic differentiation...
  20. Assessing maternal and fetal exposure to chemicals during the second trimester
    Tracey J Woodruff; Fiscal Year: 2012
    ..elective pregnancy terminations between 14 and 23 weeks gestation, including maternal serum and urine, amniotic fluid, umbilical cord blood, and placenta...
  21. Innovative Molecular Platform for Prenatal Diagnostics
    David Scott Johnson; Fiscal Year: 2011
    ..Finally, we will validate the molecular technology on archival genomic DNA, amniotic fluid, and fetal nucleated red blood cells (FNRBCs)...
  22. Developmentally Programmed Hyperphagia and Obesity via BPA enhanced Neurogenesis
    MICHAEL GLENN ROSS; Fiscal Year: 2013
    ..and paper industries, and significant levels are consistently observed in pregnant women and fetal plasma and amniotic fluid. BPA acts an endocrine disrupter (EDC), and epidemiological studies confirm that EDC exposure during ..
  23. Osteopathic Manipulative Medicine in Pregnancy: Physiologic and Clinical Effects
    KENDI LEE HENSEL; Fiscal Year: 2010
    ..in the pre and post-partum period Aim: To determine if OMM can decrease the incidence of meconium-stained amniotic fluid in labor and delivery as an index of fetal stress Study Two Hypothesis Physiological changes related to ..
  24. Pathways to shortened gestation among Black women
    SHANNON L GILLESPIE; Fiscal Year: 2013
    ..IL-1? elevations have been noted in the periphery and amniotic fluid of Black women with PTB compared to Black term controls. These findings do not extend to White populations...
  25. MATERNAL DEHYDRATION--FETAL/AMNIOTIC FLUID HOMEOSTASIS
    Michael Ross; Fiscal Year: 2002
    b>Amniotic fluid (AF) is an essential accompaniment of normal pregnancy, necessary for fetal movement, growth and development. Oligohydramnios, or reduced AF volume, occurs in 8 to 38 percent of all pregnancies...
  26. ROLE OF UREAPLASMA INTRA-AMNIOTIC INFECTION IN IUGR
    Gail Cassell; Fiscal Year: 1991
    ..This study will determine the association of intra-amniotic ureaplasmal infection with IUGR. Amniotic fluid and placenta will be quantitatively cultured for U...
  27. REGULATION OF VEGF EXPRESSION IN PLACENTA AND MEMBRANES
    CECILIA CHEUNG; Fiscal Year: 2004
    A comprehensive understanding of the control of amniotic fluid volume is of major clinical importance...
  28. Early Gestation as a Sensitive Period to Stress in Sex-Dependent Neurodevelopment
    Tracy L Bale; Fiscal Year: 2013
    ..and pathway focused PCR Arrays to analyze placental and embryonic brain tissues and proteomic analysis of amniotic fluid across gestation to identify potential translatable biomarkers and genes important in the programming of ..
  29. EXPERIMENTAL MODEL FOR CHORIOAMNIONITIS AND PREMATURITY
    Michael Gravett; Fiscal Year: 2005
    ..After postoperative stabilization in a tether, we will; (1) inoculate Group B Streptococci (GBS) into the amniotic fluid to establish intrauterine infection and preterm labor...
  30. MENTORED PATIENT ORIENTED RESEARCH CAREER DEVELOPMENT AW
    MARK STEEN; Fiscal Year: 2001
    ..Although they found lowered median cobalamin in the amniotic fluid from NTD pregnancies compared with controls, only ratios of MS-related metabolites provided biochemical ..
  31. COMPARISON OF CHORION VILLUS SAMPLING AND AMNIOCENTESIS
    ROBERT DESNICK; Fiscal Year: 1990
    ..Phases IIIb and IV) will include the confirmation of all CVS results by appropriate studies of cultured amniotic fluid cells obtained by amniocentesis, specimens from induced abortion material or specimens obtained from newborns ..
  32. Mouse Model For Amniotic Fluid Volume Regulation
    CECILIA CHEUNG; Fiscal Year: 2002
    A comprehensive understanding of the control of amniotic fluid volume is of major clinical importance...
  33. ARACHIDONATE LIPOXYGENASE ACTIVITIES IN HUMAN PREGNANCY
    Murray Mitchell; Fiscal Year: 1992
    ..The effects of hormones, bacterial products, monokines and other substances in amniotic fluid from control, and complicated (e.g. PIH, infection etc...
  34. Advanced Reproductive Age and Environmental Health
    Stephanie Engel; Fiscal Year: 2007
    ..This unique population will allow us to assess fetal exposure to EA chemicals in the amniotic fluid. The study aims are to: 1) Assemble a repository of biological specimens collected in the antenatal period; 2) ..
  35. Fetal Membrane AQP Expression/Amniotic Fluid Regulation
    Michael Ross; Fiscal Year: 2004
    b>Amniotic fluid (AF) is an essential accompaniment of normal pregnancy, necessary for fetal movement, growth and development...
  36. Pluripotency of Amniotic Fluid-Derived Stem Cells
    COLIN EDWARD BISHOP; Fiscal Year: 2010
    ..Other classes of hPSCs may overcome these limitations. In the agency-wide Program Announcement, amniotic fluid is noted specifically as a potential non-embryonic source for hPSCs...
  37. Placental Pathology: Digital Assessment and Validation
    Carolyn Salafia; Fiscal Year: 2009
    ..against measures other than "group consensus", a poor substitute for biologically valid endpoints such as amniotic fluid or cord blood proteomics...
  38. Role of the Fetus in the Initiation of Parturition
    CAROLE MENDELSON; Fiscal Year: 2004
    ..lectin involved in innate immune response, that is developmentally regulated in fetal lung and secreted into amniotic fluid near term, signals the initiation of labor...
  39. DIAGNOSTIC VIABILITY OF UMBILICAL CORD SPECIMENS
    Charles Plate; Fiscal Year: 2007
    ..Meconium is difficult to collect and in 8-20% of births in the U.S. the meconium is released into the amniotic fluid pre-birth due to some type of fetal distress...
  40. CHARACTERIZATION OF PLACENTAL OPIOID-ENHANCING FACTOR
    MARK KRISTAL; Fiscal Year: 1991
    Recent research has demonstrated, in nonpregnant rats, that consumption of placenta or amniotic fluid significantly enhances opioid-mediated analgesia, although it does not, by itself, produce analgesia...
  41. ETIOLOGY, HORMONAL CRITERIA OF 3 BETA HSD DEFICIENCY
    Songya Pang; Fiscal Year: 2002
    ..at risk by a) type II 3b-HSD gene analysis from amniotic and the proband's cells, b) hormonal analysis of amniotic fluid, c) fetal outcome verification; 4) association between the hormonal marker of mildly decreased Ad 3b-HSD ..
  42. ERYTHROPOIETIC AND HEMODYNAMIC RESPONSE TO HYPOXEMIA
    John Widness; Fiscal Year: 1990
    ..objective of this proposal is to examine developmentally related factors which may influence fetal plasma and amniotic fluid levels of erythropoietin (Ep), erythropoiesis, and regional blood flow in chronically hypoxemic fetal sheep...
  43. Imprint Osmoregulation/Maternally-Dehydrated Offspring
    Mina Desai; Fiscal Year: 2007
    ..fetal plasma osmolality, which stimulates fetal arginine vasopressin (AVP) secretion, causing reduced amniotic fluid (AF) volume...
  44. ULTRASOUND SCREENING AND RISK ASSESSMENT OF PREGNANCY
    Fredric Frigoletto; Fiscal Year: 1993
    ..in later pregnancy (32-36 weeks) to detect abnormalities of fetal growth, fetal presentation, and placental or amniotic fluid abnormalities...
  45. RADIOASSAY OF ERYTHROPOIETIN
    GISELA CLEMONS; Fiscal Year: 1991
    ..Fetal hypoxemia in high risk pregnancies can be diagnosed by measuring the hormone level in the amniotic fluid. In addition to human erythropoietin a variety of animal erythropoietins are measurable with this assay...
  46. COMPARISON OF CHORION VILLUS SAMPLING AND AMNIOCENTESIS
    Eugene Pergament; Fiscal Year: 1990
    ..be tested are whether the genetic analyses performed on chorionic villi are as accurate as those performed on amniotic fluid cells, particularly in the identification of chromosomally abnormal conceptions; and, whether CVS is ..
  47. CYTOMATION MOFLO MLS FLOW CYTOMETER
    Michael Axthelm; Fiscal Year: 1999
    ..and assess the functions of cell subpopulations in peripheral blood, lymph nodes, thymus, bone marrow, amniotic fluid, chorion and decidua, corpus luteum, and human, monkey and mouse brain...
  48. MOLECULAR CYTOGENETICS IN PRE AND NEONATAL DIAGNOSIS
    Daniel Pinkel; Fiscal Year: 1999
    ..from the hundreds to thousands of cells that can be obtained without cell culture from a few milliliters of amniotic fluid. This will require: a) Further development of CGHx procedures and analysis software; b) Development/..
  49. F. nucleatum in periodontal disease and preterm birth
    Yiping W Han; Fiscal Year: 2010
    ..nudeatum-hosl cell interactions;and (iii) to determine if women with amniotic fluid infections of F. nucleatum harbor the same species in the vaginal track, blood, or the oral cavity.
  50. INTERLEUKIN-1 IN HUMAN PREGNANCY
    Roberto Romero; Fiscal Year: 1991
    ..We will: 1) physically characterize IL-1-like activity in amniotic fluid and decidua; 2) describe the kinetics and cellular sources of IL-1-like activity in decidua; 3) determine the ..
  51. Renal Tissue Derived From Amniotic Fluid Stem Cells
    ROGER EDWARD DE FILIPPO; Fiscal Year: 2010
    ..We have discovered that a unique population of pluripotential cells exist in amniotic fluid that have similar totipotent properties as more traditional lines of embryonic stem cells but avoid embryo ..
  52. FETAL CIRCULATORY RESPONSE TO CEREBRAL COMPRESSION
    Raymond Koehler; Fiscal Year: 1991
    ..Because of the compliant fetal skull, these forces can cause intracranial pressure (ICP) to greatly exceed amniotic fluid pressure, thereby decreasing cerebral perfusion pressure. Thus, cerebral blood flow (CBF) could decrease...
  53. PATHOGENESIS OF PULMONARY HYPOPLASIA IN CHONDRODYSTROPHY
    Robert Seegmiller; Fiscal Year: 1990
    ..experimentally induced by paralysis of the fetus, obstruction of the trachea and urinary tract, and removal of amniotic fluid. These experiments, while instructive, have their limitations...
  54. RANDOMIZED TRIAL OF 11-14 WEEK AMNIOCENTESIS AND TA CVS
    Elizabeth Thom; Fiscal Year: 1999
    ..a combined endpoint of fetal loss or preterm delivery before 196 days gestation and also by total fetal loss, amniotic fluid, gestational age at delivery, perinatal morbidity, neonatal morbidity and congenital abnormalities, including ..
  55. Role of Intrauterine Ureaplasma-Infection in BPD Pathogenesis
    ROSE MARIE VISCARDI; Fiscal Year: 2010
    ..Ureaplasma parvum and U. urealyticum are the most common organisms isolated from infected amniotic fluid and infant tracheal aspirates (3-5)...
  56. REGULATION OF AMNIOTIC FLUID VOLUME
    ROBERT BRACE; Fiscal Year: 1992
    ..objective of the proposed studies is to understand the detail mechanisms involved in the regulation of amniotic fluid volume under normal as well as abnormal conditions...
  57. BIOMOLECULAR BASIS OF INFECTION-INDUCED PRETERM LABOR
    Susan Cox; Fiscal Year: 1990
    ..necrosis factor/cachectin (TNF-alpha), interleukin-1 (IL-1), and colony stimulating factor(s) (CSF(s)) in amniotic fluid and (ii) to ascertain if bioactive agents of infection (LPS or selected cytokines or both), which may ..
  58. Development Psychopharmacology
    DONALD NEWPORT; Fiscal Year: 2005
    ..exposure via collection of breast milk and serum of nursing infants, and prenatal exposure via collection of amniotic fluid in the event of amniocentesis...
  59. ROLE OF CATECHOLAMINES IN THE INITIATION OF LABOR
    CHARLES DUCSAY; Fiscal Year: 1990
    ..Studies will include measurements of epinephrine, and dopamine in maternal and fetal plasma and amniotic fluid. In addition, PG concentrations (PGEM-II, PGFM, and 6-keto-PGF1alpha) will be quantitated...
  60. PRENATAL INFECTION, BRAIN DEVELOPMENT AND SCHIZOPHRENIA
    John Gilmore; Fiscal Year: 2003
    ..b>Amniotic fluid and fetal cord blood levels of cytokines are increased in human pregnancies complicated by infection...
  61. Cell therapy of diabetes using broad spectrum multipotent stem cells
    Shay Soker; Fiscal Year: 2007
    ..A promising new source of stem cells has been identified, namely, amniotic fluid collected for prenatal genetic testing...
  62. PLACENTAL PROTEIN AND POLYPEPTIDE HORMONES
    Robert Shiu; Fiscal Year: 1993
    ..To determine where these proteins circulate - maternal serum, fetal serum, amniotic fluid - and at what levels, radioimmunoassays will be established...
  63. MECHANISMS OF EGF ACTION IN THE FETUS AND NEONATE
    Dennis Styne; Fiscal Year: 1993
    ..To elucidate mechanisms of action of EGF in fetal monkeys and to differentiate the responses to amniotic fluid (oral) and systemic (parenteral) administration of EGF in fetal Rhesus monkeys. 2...
  64. FEASIBILITY OF PRENATAL SCREENING FOR SLO SYNDROME
    George Knight; Fiscal Year: 2003
    ..the diagnosis biochemically by measuring cholesterol precursors in the serum of affected individuals and in amniotic fluid. Secondly, the array of maternal serum analytes currently measured routinely to screen for Down syndrome in 2,..
  65. FETAL IMMUNOPROPHYLAXIS AGAINST A PRIMATE LENTIVIRUS
    Ruth Ruprecht; Fiscal Year: 2001
    ..Neutralizing mAbs levels will be measured in maternal blood, amniotic fluid, cord blood and in neonatal mucosal secretions after passive therapy of pregnant macaque dams and newborns. 3...
  66. Tissue-Specific Extracellular Matrix for Culturing Stem and Progenitor Cells
    Mark Van Dyke; Fiscal Year: 2006
    ..dermis ECM), 2) muscle satellite cells differentiated into skeletal myoblasts (skeletal muscle ECM), and 3) amniotic fluid-derived stem cells differentiated into hepatocytes (liver ECM)...
  67. DIFFERENTIATION IN HUMAN AMNIOTIC FLUID CELLS
    ROBERT PRIEST; Fiscal Year: 1980
    This proposal is to characterize further the different types of cells that can be cultured from human amniotic fluid. Collagen synthesized by fibroblastic (F type) and a more frequently found type of cell (AF type) will be characterized ..
  68. Diagnostic proteomic profiles for aneuploidy
    Ashok Reddy; Fiscal Year: 2004
    ..5%-1.0% risk of miscarriage. Furthermore, chromosomal analysis of amniotic fluid cells is a labor-intensive and time-consuming procedure, taking up to two weeks...
  69. PATHOPHYSIOLOGY OF PULMONARY SURFACTANT
    Sikandar Katyal; Fiscal Year: 1991
    ..maturity and the risk of respiratory distress syndrome (RDS) can be predicted by the assay of SAP-A in the amniotic fluid samples...
  70. HLA G TEST FOR RUPTURE OF MEMBRANES AND PRETERM DELIVERY
    Simon Shorter; Fiscal Year: 2000
    ..During Phase I of this project the investigators found that HLA-G is easily detectable, by immunoblotting, in amniotic fluid. This suggests that HLA-G measurements could be used in diagnostic assays that detect amniotic fluid analytes...
  71. IL-1 RESPIRATORY ADAPTATION IN THE PERINATAL PERIOD
    Kristina Bry; Fiscal Year: 2001
    ..Increased concentration of the proinflammatory cytokine interleukin-l (IL-1) in amniotic fluid has been shown to be a risk factor for the development of BPD in the newborn infant...