oocytes

Summary

Summary: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Top Publications

  1. ncbi The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes
    Tian Peng Gu
    Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Nature 477:606-10. 2011
  2. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
  3. ncbi Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice
    Katsuhiko Hayashi
    Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Science 338:971-5. 2012
  4. pmc Dynamic CpG island methylation landscape in oocytes and preimplantation embryos
    Sebastien A Smallwood
    Epigenetics Programme, The Babraham Institute, Cambridge, UK
    Nat Genet 43:811-4. 2011
  5. ncbi TMEM16A confers receptor-activated calcium-dependent chloride conductance
    Young Duk Yang
    Sensory Research Center, CRI, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    Nature 455:1210-5. 2008
  6. ncbi The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
    R Rohatgi
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 97:221-31. 1999
  7. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
  8. pmc Site specific mutation of the Zic2 locus by microinjection of TALEN mRNA in mouse CD1, C3H and C57BL/6J oocytes
    Benjamin Davies
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 8:e60216. 2013
  9. ncbi The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes
    J E Ferrell
    Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305 5332, USA
    Science 280:895-8. 1998
  10. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002

Detail Information

Publications337 found, 100 shown here

  1. ncbi The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes
    Tian Peng Gu
    Group of DNA Metabolism, The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Nature 477:606-10. 2011
    ..b>Oocytes lacking Tet3 also seem to have a reduced ability to reprogram the injected nuclei from somatic cells...
  2. ncbi CRM1 is an export receptor for leucine-rich nuclear export signals
    M Fornerod
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1051-60. 1997
    ..Overexpression of CRM1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus...
  3. ncbi Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice
    Katsuhiko Hayashi
    Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida konoe cho, Sakyo ku, Kyoto 606 8501, Japan
    Science 338:971-5. 2012
    ..transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization...
  4. pmc Dynamic CpG island methylation landscape in oocytes and preimplantation embryos
    Sebastien A Smallwood
    Epigenetics Programme, The Babraham Institute, Cambridge, UK
    Nat Genet 43:811-4. 2011
    ..Here we present, to our knowledge, the first integrated epigenomic analysis of mammalian oocytes, identifying over a thousand CGIs methylated in mature oocytes...
  5. ncbi TMEM16A confers receptor-activated calcium-dependent chloride conductance
    Young Duk Yang
    Sensory Research Center, CRI, College of Pharmacy, Seoul National University, Seoul 151 742, Korea
    Nature 455:1210-5. 2008
    ..We conclude that ANO1 is a candidate Ca(2+)-activated chloride channel that mediates receptor-activated chloride currents in diverse physiological processes...
  6. ncbi The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
    R Rohatgi
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 97:221-31. 1999
    ..Therefore, N-WASP and the Arp2/3 complex comprise a core mechanism that directly connects signal transduction pathways to the stimulation of actin polymerization...
  7. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
    ..The simultaneous absence of the reticulons and Yop1p in S. cerevisiae results in disrupted tubular ER. We propose that these "morphogenic" proteins partition into and stabilize highly curved ER membrane tubules...
  8. pmc Site specific mutation of the Zic2 locus by microinjection of TALEN mRNA in mouse CD1, C3H and C57BL/6J oocytes
    Benjamin Davies
    Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
    PLoS ONE 8:e60216. 2013
    ....
  9. ncbi The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes
    J E Ferrell
    Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305 5332, USA
    Science 280:895-8. 1998
    Xenopus oocytes convert a continuously variable stimulus, the concentration of the maturation-inducing hormone progesterone, into an all-or-none biological response-oocyte maturation...
  10. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
    ..Paternal demethylation in fertilised oocytes deficient for MBD2, the only candidate demethylase, occurred normally...
  11. pmc Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes
    Shao Chen Sun
    Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea
    PLoS ONE 6:e18392. 2011
    ..Our data suggest that the Arp2/3 complex probably regulates oocyte polarization through its effect on spindle migration, asymmetric division and cytokinesis during mouse oocyte meiotic maturation...
  12. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    ..We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor...
  13. pmc The pannexin 1 channel activates the inflammasome in neurons and astrocytes
    William R Silverman
    Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 284:18143-51. 2009
    ..adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD) blocked ATP-induced cell death in oocytes co-expressing P2X7 receptor and pannexin 1...
  14. ncbi A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel
    M C Sanguinetti
    Eccles Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City 84112, USA
    Cell 81:299-307. 1995
    ..To define the function of HERG, we expressed the protein in Xenopus oocytes. The biophysical properties of expressed HERG are nearly identical to the rapidly activating delayed rectifier K+ ..
  15. ncbi A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes
    Markus T Bohnsack
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, INF 282, D 69120 Heidelberg, Germany
    Nat Cell Biol 8:257-63. 2006
    ..Indeed, Exp6 is undetectable in oocytes, but is synthesized from meiotic maturation onwards, which explains how actin export resumes later in ..
  16. ncbi Insect olfactory receptors are heteromeric ligand-gated ion channels
    Koji Sato
    Department of Integrated Biosciences, The University of Tokyo, Chiba 277 8562, Japan
    Nature 452:1002-6. 2008
    ..The ligand-gated ion channel formed by an insect OR complex seems to be the basis for a unique strategy that insects have acquired to respond to the olfactory environment...
  17. ncbi TGF-beta superfamily members and ovarian follicle development
    Phil G Knight
    School of Biological Sciences, The University of Reading, Whiteknights, Reading RG6 6AJ, UK
    Reproduction 132:191-206. 2006
    ..to the transforming growth factor-beta (TGF-beta ) superfamily, are expressed by ovarian somatic cells and oocytes in a developmental, stage-related manner and function as intraovarian regulators of folliculogenesis...
  18. ncbi Mitochondrial DNA content affects the fertilizability of human oocytes
    P Reynier
    INSERM EMI U 00 18, Laboratoire de Biochimie et Biologie Moleculaire, CHU d Angers, F 49033 Angers, pavillon de la mère et de l enfant, CHU de Nantes, B P 1005, F 44093 Nantes cedex 1, France
    Mol Hum Reprod 7:425-9. 2001
    Mitochondrial DNA content varies considerably in oocytes, even when collected from the same patient...
  19. ncbi Derivation of oocytes from mouse embryonic stem cells
    Karin Hübner
    Germline Development Group, Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center, 382 West Street Road, Kennett Square, PA 19348, USA
    Science 300:1251-6. 2003
    ..Oogenesis in culture should contribute to various areas, including nuclear transfer and manipulation of the germ line, and advance studies on fertility treatment and germ and somatic cell interaction and differentiation...
  20. ncbi Symmetry breaking in mouse oocytes requires transient F-actin meshwork destabilization
    Jessica Azoury
    UMR7622, CNRS UMPC, College de France, 9 quai Saint Bernard, Paris, France
    Development 138:2903-8. 2011
    ..This asymmetry is achieved via off-center positioning of the division spindle. Mouse oocytes have developed a formin-2-dependent actin-based spindle positioning mechanism that allows the meiotic spindle to ..
  21. pmc Dynamic stage-specific changes in imprinted differentially methylated regions during early mammalian development and prevalence of non-CpG methylation in oocytes
    Shin Ichi Tomizawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Japan
    Development 138:811-20. 2011
    ..methylation, we determined and compared methylation patterns of the corresponding regions in sperm and oocytes. We found that the extent of the gametic DMRs differs significantly from that of the embryonic DMRs, especially ..
  22. pmc Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes
    Toshiaki Watanabe
    Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
    Genes Dev 20:1732-43. 2006
    ..One class consists of approximately 20- to 24-nt small interfering RNAs (siRNAs) from mouse oocytes, which are derived from retroelements including LINE, SINE, and LTR retrotransposons...
  23. ncbi Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer
    Satoshi Kishigami
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, 2 2 3 Minatojima minamimachi, Kobe 650 0047, Japan
    Biochem Biophys Res Commun 340:183-9. 2006
    ..Thus, our data indicate that TSA-treatment after SCNT in mice can dramatically improve the practical application of current cloning techniques...
  24. pmc The human VASA gene is specifically expressed in the germ cell lineage
    D H Castrillon
    Women s and Perinatal Pathology Division, Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:9585-90. 2000
    ..in fetal and adult gonadal germ cells in both males and females and is most abundant in spermatocytes and mature oocytes. The gene we have isolated is thus a highly specific marker of germ cells and should be useful for studies of ..
  25. pmc Transcription is required for establishment of germline methylation marks at imprinted genes
    Mita Chotalia
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB22 3AT, United Kingdom
    Genes Dev 23:105-17. 2009
    ..Transcription through DMRs in oocytes is not restricted to this locus but occurs across the prospective DMRs at many other maternally marked imprinted ..
  26. ncbi Pannexin membrane channels are mechanosensitive conduits for ATP
    Li Bao
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 572:65-8. 2004
    ..They have a wide expression spectrum, they are of large conductance and permeant for ATP, and they are mechanosensitive. Hence, pannexins are candidates for the release of ATP to the extracellular space upon mechanical stress...
  27. pmc Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development
    Ryutaro Hirasawa
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Genes Dev 22:1607-16. 2008
    ..Consistent with these results we find that zygotic Dnmt1 is expressed in the preimplantation embryo. Thus, contrary to the previous reports, Dnmt1 alone is sufficient to maintain the methylation marks of the imprinted genes...
  28. ncbi KCNQ2 and KCNQ3 potassium channel subunits: molecular correlates of the M-channel
    H S Wang
    Institute of Molecular Cardiology, Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    Science 282:1890-3. 1998
    ..The biophysical properties, sensitivity to pharmacological blockade, and expression pattern of the KCNQ2 and KCNQ3 potassium channels were determined. It is concluded that both these subunits contribute to the native M-current...
  29. ncbi Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice
    Kenichiro Hata
    Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
    Development 129:1983-93. 2002
    ..Together, our findings suggest that Dnmt3L may cooperate with Dnmt3 family methyltransferases to carry out de novo methylation of maternally imprinted genes in oocytes.
  30. ncbi Subunit arrangement and function in NMDA receptors
    Hiroyasu Furukawa
    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, New York, New York 10032, USA
    Nature 438:185-92. 2005
    ....
  31. ncbi Silencing of unsynapsed meiotic chromosomes in the mouse
    James M A Turner
    Division of Stem Cell Research and Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nat Genet 37:41-7. 2005
    ..These findings impact on the interpretation of the relationship between synaptic errors and sterility in mammals and extend our understanding of the biology of Brca1...
  32. pmc Proteome of mouse oocytes at different developmental stages
    Shufang Wang
    National Institute of Biological Sciences, Beijing 102206, People s Republic of China
    Proc Natl Acad Sci U S A 107:17639-44. 2010
    ..transcripts may enhance the generation of induced pluripotent stem cells, the reprogramming factors in oocytes are undetermined, and even the identified proteins composition of oocytes is very limited...
  33. ncbi Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes
    Melina Schuh
    Gene Expression Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 130:484-98. 2007
    Chromosome segregation in mammalian oocytes is driven by a microtubule spindle lacking centrosomes. Here, we analyze centrosome-independent spindle assembly by quantitative high-resolution confocal imaging in live maturing mouse oocytes...
  34. pmc Maternal control of early mouse development
    Lei Li
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA
    Development 137:859-70. 2010
    ..Nevertheless, significant gaps remain in our mechanistic understanding of the networks that regulate early mammalian embryogenesis, which provide an impetus and opportunities for future investigations...
  35. pmc Detection, characterization, and spontaneous differentiation in vitro of very small embryonic-like putative stem cells in adult mammalian ovary
    Seema Parte
    Department of Stem Cell Biology, National Institute for Research in Reproductive Health, Mumbai, India
    Stem Cells Dev 20:1451-64. 2011
    ..In conclusion, as opposed to the existing view of OSE being a bipotent source of oocytes and granulosa cells, mammalian ovaries harbor distinct very small embryonic-like PSCs and tissue committed ..
  36. pmc Pannexin 1 in erythrocytes: function without a gap
    Silviu Locovei
    Department of Physiology and Biophysics, University of Miami Miller School of Medicine, P O Box 016430, Miami, FL 33101, USA
    Proc Natl Acad Sci U S A 103:7655-9. 2006
    ..This protein, in addition to forming gap junction channels in paired oocytes, can also form a mechanosensitive and ATP-permeable channel in the nonjunctional plasma membrane...
  37. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
    ..Mouse oocytes lacking Dicer arrest in meiosis I with multiple disorganized spindles and severe chromosome congression defects...
  38. pmc Novel signaling mechanisms in the ovary during oocyte maturation and ovulation
    Marco Conti
    Center for Reproductive Sciences, Department of Obstetrics and Gynecology and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, United States
    Mol Cell Endocrinol 356:65-73. 2012
    ..This review will provide a summary of the current knowledge of the molecular events triggered by LH focusing mostly on the signaling pathways required for oocyte maturation...
  39. pmc Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes
    Liming Gui
    Cell and Developmental Biology, University College London, London WC1E 6BT, UK
    Development 139:1941-6. 2012
    ..Paradoxically, although an SAC operates in mammalian oocytes, meiosis I (MI) is notoriously error prone and polar-displaced chromosomes do not prevent anaphase onset...
  40. pmc Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process?
    Jerome Jullien
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nat Rev Mol Cell Biol 12:453-9. 2011
    ..These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes...
  41. ncbi Germline stem cells and follicular renewal in the postnatal mammalian ovary
    Joshua Johnson
    Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital, and Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 428:145-50. 2004
    ..females lose the capacity for germ-cell renewal during fetal life, such that a fixed reserve of germ cells (oocytes) enclosed within follicles is endowed at birth...
  42. ncbi p63 protects the female germ line during meiotic arrest
    Eun Kyung Suh
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 444:624-8. 2006
    ..How DNA damage is detected in these arrested oocytes is poorly understood, but it is variably thought to involve p53, a central tumour suppressor in mammals...
  43. ncbi Complete kinetochore tracking reveals error-prone homologous chromosome biorientation in mammalian oocytes
    Tomoya S Kitajima
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, Heidelberg D 69117, Germany
    Cell 146:568-81. 2011
    ..3D kinetochore-tracking datasets throughout cell division by high-resolution imaging of meiosis I in live mouse oocytes. We show that in acentrosomal oocytes, chromosome congression forms an intermediate chromosome configuration, the ..
  44. ncbi Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells
    Oliver J Gruss
    EMBL, Meyerhofstrasse 1, 69 117 Heidelberg, Germany
    Nat Cell Biol 4:871-9. 2002
    ..This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells...
  45. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
    ....
  46. pmc Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women
    Yvonne A R White
    Vincent Center for Reproductive Biology, Massachusetts General Hospital Vincent Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, Massachusetts, USA
    Nat Med 18:413-21. 2012
    Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries...
  47. ncbi Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog
    D L Pittman
    Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Mol Cell 1:697-705. 1998
    ..The strong similarity of phenotypes in Dmc1-deficient mice and yeast suggests that meiotic mechanisms have been highly conserved through evolution...
  48. pmc The low-affinity monocarboxylate transporter MCT4 is adapted to the export of lactate in highly glycolytic cells
    K S Dimmer
    Physiologisches Institut der Universitat, Gmelinstr 5, D 72076 Tubingen, Germany
    Biochem J 350:219-27. 2000
    ..To analyse the functional properties of this transporter, rat MCT4 was expressed in Xenopus laevis oocytes and transport activity was monitored by flux measurements with radioactive tracers and by changes of the ..
  49. ncbi Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner
    S M Galloway
    AgResearch Molecular Biology Unit, and Centre for Gene Research, Department of Biochemistry, University of Otago, Dunedin, New Zealand
    Nat Genet 25:279-83. 2000
    ..BMP15 is a member of the transforming growth factor beta (TGFbeta) superfamily and is specifically expressed in oocytes, its function is unknown. We show that independent germline point mutations exist in FecXI and FecXH carriers...
  50. pmc Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes
    Kei Miyamoto
    Wellcome Trust, Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Genes Dev 25:946-58. 2011
    Amphibian oocytes can rapidly and efficiently reprogram the transcription of transplanted somatic nuclei...
  51. ncbi Controls of germline stem cells, entry into meiosis, and the sperm/oocyte decision in Caenorhabditis elegans
    Judith Kimble
    Department of Biochemistry and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706 1544, USA
    Annu Rev Cell Dev Biol 23:405-33. 2007
    ..Parallels with controls in other organisms include the use of PUF proteins for stem cell maintenance and the prominence of mRNA regulation for the control of germline development...
  52. ncbi Inhibition of the c-Abl-TAp63 pathway protects mouse oocytes from chemotherapy-induced death
    Stefania Gonfloni
    Department of Biology, University of Rome Tor Vergata, Rome, Italy
    Nat Med 15:1179-85. 2009
    ..the c-Abl-TAp63 pathway activated by chemotherapeutic DNA-damaging drugs in model human cell lines and in mouse oocytes and its role in cell death...
  53. ncbi Molecular and functional heterogeneity of hyperpolarization-activated pacemaker channels in the mouse CNS
    B Santoro
    Center for Neurobiology and Behavior, Departments of Pharmacology and Anesthesiology, and Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    J Neurosci 20:5264-75. 2000
    ..Moreover, in some neurons, specific functional roles can be proposed for I(h) channels with defined subunit composition...
  54. ncbi Putative stem cells with an embryonic character isolated from the ovarian surface epithelium of women with no naturally present follicles and oocytes
    Irma Virant-Klun
    Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, Ljubljana, Slovenia
    Differentiation 76:843-56. 2008
    ..study was to isolate the putative ovarian stem cells (OSCs) from the OSE layer in women with no naturally present oocytes and follicles--20 postmenopausal women and five women with premature ovarian failure...
  55. ncbi Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
    Robert B Gilchrist
    Research Centre for Reproductive Health, School of Paediatrics and Reproductive Health, Discipline of Obstetrics and Gynaecology, Medical School, University of Adelaide, Adelaide 5005, Australia
    Hum Reprod Update 14:159-77. 2008
    ..Hence, oocytes appear to tightly control their neighboring somatic cells, directing them to perform functions required for ..
  56. pmc In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization
    Vitaly L Zimyanin
    The Gurdon Institute and the Department of Genetics, University of Cambridge, Tennis Court Road, CB2 1QN Cambridge, UK
    Cell 134:843-53. 2008
    ..By following oskar mRNA particles in living oocytes, we show that the mRNA is actively transported along microtubules in all directions, with a slight bias toward ..
  57. ncbi Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation
    Penghui Ai
    State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China
    Plant J 57:798-809. 2009
    ..Xenopus oocytes injected with OsPT2 mRNA showed increased Pi accumulation and a Pi-elicited depolarization of the cell membrane ..
  58. pmc A gene expression signature shared by human mature oocytes and embryonic stem cells
    Said Assou
    CHU Montpellier, Institute for Research in Biotherapy, Hopital Saint Eloi, Montpellier, F 34000 France
    BMC Genomics 10:10. 2009
    ..Human mature MII oocytes and embryonic stem (ES) cells are both able to achieve the feat of cell reprogramming towards pluripotency, ..
  59. ncbi Pharmacological properties of homomeric and heteromeric pannexin hemichannels expressed in Xenopus oocytes
    Roberto Bruzzone
    Department of Neuroscience, Institut Pasteur, Paris, France
    J Neurochem 92:1033-43. 2005
    ..The opposite was true in the case of connexin46 (Cx46), thus indicating that gap junction blockers are able to selectively modulate pannexin and connexin channels...
  60. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
    ..Here we show that a subset of pseudogenes generates endogenous small interfering RNAs (endo-siRNAs) in mouse oocytes. These endo-siRNAs are often processed from double-stranded RNAs formed by hybridization of spliced transcripts ..
  61. ncbi HERG, a human inward rectifier in the voltage-gated potassium channel family
    M C Trudeau
    Department of Physiology, University of Wisconsin Medical School, Madison 53706, USA
    Science 269:92-5. 1995
    ..Because mutations in HERG cause a form of long-QT syndrome, these properties of HERG channel function may be critical to the maintenance of normal cardiac rhythmicity...
  62. ncbi The molecular control of meiotic chromosomal behavior: events in early meiotic prophase in Drosophila oocytes
    Cathleen M Lake
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Annu Rev Physiol 74:425-51. 2012
    We review the critical events in early meiotic prophase in Drosophila melanogaster oocytes. We focus on four aspects of this process: the formation of the synaptonemal complex (SC) and its role in maintaining homologous chromosome ..
  63. pmc Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes
    J Miller
    EMBO J 4:1609-14. 1985
    The 7S particle of Xenopus laevis oocytes contains 5S RNA and a 40-K protein which is required for 5S RNA transcription in vitro...
  64. ncbi A new model for asymmetric spindle positioning in mouse oocytes
    Melina Schuh
    European Molecular Biology Laboratory EMBL, Gene Expression Unit, Heidelberg, Germany
    Curr Biol 18:1986-92. 2008
    ..To divide asymmetrically, mammalian oocytes relocate the spindle from their center to the cortex...
  65. ncbi Small-conductance, calcium-activated potassium channels from mammalian brain
    M Kohler
    Vollum Institute, L 474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Road, Portland, OR 97201, USA J Maylie, Department of Obstetrics and Gyne
    Science 273:1709-14. 1996
    ..Expression of the messenger RNAs in Xenopus oocytes resulted in calcium-activated, voltage-independent potassium channels...
  66. pmc Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex
    Silviu Locovei
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 581:483-8. 2007
    ..Expression of P2X(7)R in Xenopus oocytes results exclusively in formation of a non-selective cation channel...
  67. pmc The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites
    Priscila V Castilho
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 20:4777-89. 2009
    ..These findings support the hypothesis that M phase requires not only high levels of MPF function, but also the suppression, through a Gwl-dependent mechanism, of phosphatase(s) that would otherwise remove MPF-driven phosphorylations...
  68. ncbi Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes
    Hisataka Iwata
    Tokyo University of Agriculture, Funako 1737, Atugi City 243 0034, Japan
    Reprod Fertil Dev 23:424-32. 2011
    ..of maternal age (in months) on mitochondrial DNA copy number (Mt number), ATP content and IVF outcome of bovine oocytes. We also compared the Mt number of oocytes with fertilisation outcome and ATP content...
  69. pmc New insights into the genetic regulation of homologue disjunction in mammalian oocytes
    H Homer
    Mammalian Oocyte and Embryo Research Laboratory, Department of Cell and Developmental Biology, Division of Biosciences and Institute for Women s Health, University College London, London, UK
    Cytogenet Genome Res 133:209-22. 2011
    Mammalian oocytes execute a unique meiotic programme involving 2 arrest stages and an unusually protracted preamble to chromosome segregation during the first meiotic division (meiosis I)...
  70. ncbi Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
    Toshiaki Watanabe
    Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima 411 8540, Japan
    Nature 453:539-43. 2008
    ..Here we show, using mouse oocytes, that endogenous siRNAs are derived from naturally occurring dsRNAs and have roles in the regulation of gene ..
  71. ncbi Crystal structure and functional analysis of the HERG potassium channel N terminus: a eukaryotic PAS domain
    J H Morais Cabral
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, New York, New York 10021, USA
    Cell 95:649-55. 1998
    ..The presence of the domain attached to the channel slows the rate of deactivation. Given the roles of PAS domains in biology, we propose that the HERG N-terminal domain has a regulatory function...
  72. pmc Posttranscriptional activation of gene expression in Xenopus laevis oocytes by microRNA-protein complexes (microRNPs)
    Richard D Mortensen
    Cancer Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 108:8281-6. 2011
    ..e., G0) mammalian cell lines. Induced quiescent cells, like folliculated immature oocytes, have high levels of cAMP that activate protein kinase AII (PKAII) to maintain G0 and immature states...
  73. ncbi Synaptonemal complex-dependent centromeric clustering and the initiation of synapsis in Drosophila oocytes
    Satomi Takeo
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Curr Biol 21:1845-51. 2011
    ..Our data support a view in which the SC-mediated clustering at the centromeres is the initiating event for meiotic synapsis...
  74. ncbi Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials
    Ana Cobo
    Instituto Valenciano de Infertilidad, Valencia, Spain
    Fertil Steril 96:277-85. 2011
    ..To perform a systematic review of the literature to identify randomized controlled trials assessing the efficacy of oocyte vitrification in terms of oocyte survival, fertilization, embryo development, and pregnancy rates...
  75. pmc Two separate interfaces between the voltage sensor and pore are required for the function of voltage-dependent K(+) channels
    Seok Yong Lee
    Howard Hughes Medical Institute, Rockefeller University, New York, NY, USA
    PLoS Biol 7:e47. 2009
    ..This second interface is well positioned to act as a second anchor point between the voltage sensor and the pore, thus allowing efficient transmission of conformational changes to the pore's gate...
  76. ncbi Production of offspring from a germline stem cell line derived from neonatal ovaries
    Kang Zou
    School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
    Nat Cell Biol 11:631-6. 2009
    ..These findings contribute to basic research into oogenesis and stem cell self-renewal and open up new possibilities for use of FGSCs in biotechnology and medicine...
  77. ncbi Parthenogenetic embryo-like structures in the human ovarian surface epithelium cell culture in postmenopausal women with no naturally present follicles and oocytes
    Irma Virant-Klun
    Department of Obstetrics and Gynecology, University Medical Centre, Ljubljana, Slovenia
    Stem Cells Dev 18:137-49. 2009
    ..possible formation of parthenogenetic embryos in 21 postmenopausal women with no naturally present follicles and oocytes. Small round cells with a bubble-like structure and with a diameter from 2 to 4 microm were isolated from the ..
  78. pmc Properties of hyperpolarization-activated pacemaker current defined by coassembly of HCN1 and HCN2 subunits and basal modulation by cyclic nucleotide
    S Chen
    Department of Pharmacology, ColumbiaUniversity, New York, New York 10032, USA
    J Gen Physiol 117:491-504. 2001
    ..When expressed in Xenopus oocytes, HCN1 channels activate 5-10-fold more rapidly than HCN2 channels...
  79. ncbi Intercellular communication in the mammalian ovary: oocytes carry the conversation
    Martin M Matzuk
    Department of Pathology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Science 296:2178-80. 2002
    ..The growth of oocytes within ovarian follicles and their development to mature eggs have fascinated biologists for centuries, and ..
  80. ncbi Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities
    L Liang
    Department of Biology, McGill University, Montreal, Quebec, Canada
    Development 120:1201-11. 1994
    ..Initial recruitment of vasa to the pole plasm must consequently depend upon protein-protein interactions but, once localized, vasa must bind to RNA to mediate germ cell formation...
  81. ncbi Identification of receptors of main sex-pheromone components of three Lepidopteran species
    Hidefumi Mitsuno
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
    Eur J Neurosci 28:893-902. 2008
    ..By two-electrode voltage-clamp recording, we tested the ligand specificity of Xenopus oocytes co-expressing PxOR1, MsOR1 or DiOR1 with an OR83b family protein...
  82. pmc dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells
    Jana Nejepinska
    Institute of Molecular Genetics AS CR, Videnska 1083, 14220 Prague 4, Czech Republic
    Nucleic Acids Res 40:399-413. 2012
    ..The dsRNA was poorly processed into siRNAs in somatic cells, whereas, robust RNAi effects were found in oocytes, suggesting that somatic cells lack some factor(s) facilitating siRNA biogenesis...
  83. pmc Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 A resolution
    Xiao Tao
    Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA
    Science 326:1668-74. 2009
    ..These same surface features also suggest a possible approach to the development of inhibitory agents specific to each member of the inward-rectifier K+ channel family...
  84. pmc Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes
    Kexi Yi
    Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA
    Nat Cell Biol 13:1252-8. 2011
    Mature mammalian oocytes are poised for completing meiosis II (MII) on fertilization by positioning the spindle close to an actomyosin-rich cortical cap...
  85. pmc The S4-S5 linker couples voltage sensing and activation of pacemaker channels
    J Chen
    Department of Medicine, Division of Cardiology, Eccles Program in Human Molecular Biology and Genetics, University of Utah, Eccles Institute of Human Genetics, 15 N 2030 E, Room 4220, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 98:11277-82. 2001
    ..Our findings also suggest that opening of HCN and related channels corresponds to activation of a gate located near the inner pore, rather than recovery of channels from a C-type inactivated state...
  86. ncbi Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method
    Masashige Kuwayama
    Kato Ladies Clinic, 7 20 3 Nishishinjuku, Shinjuku, Tokyo, Japan
    Theriogenology 67:73-80. 2007
    ....
  87. pmc Maternal BRG1 regulates zygotic genome activation in the mouse
    Scott J Bultman
    Department of Genetics and the Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Genes Dev 20:1744-54. 2006
    ..In conditional-mutant females, BRG1-depleted oocytes completed meiosis and were fertilized...
  88. ncbi Ovarian aging: mechanisms and clinical consequences
    F J Broekmans
    Department of Reproductive Medicine and Gynecology, University Medical Center, Utrecht, The Netherlands
    Endocr Rev 30:465-93. 2009
    ..Improved knowledge of the ovarian ageing mechanisms may ultimately provide tools for prediction of menopause and manipulation of the early steps of folliculogenesis for the purpose of contraception and fertility lifespan extension...
  89. ncbi Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB
    W N Zagotta
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, CA 94305
    Science 250:568-71. 1990
    ..20 residues of the ShB alternatively spliced variant, to noninactivating mutant channels expressed in Xenopus oocytes. The peptide restored inactivation in a concentration-dependent manner...
  90. ncbi Activation of pannexin 1 channels by ATP through P2Y receptors and by cytoplasmic calcium
    Silviu Locovei
    Department of Physiology and Biophysics, University of Miami, School of Medicine, P O Box 016430, 1600 NW 10th Avenue, Miami, FL 33136, USA
    FEBS Lett 580:239-44. 2006
    ..Based on its properties, including ATP permeability, pannexin 1 may be involved in both initiation and propagation of calcium waves...
  91. pmc A bivalent tarantula toxin activates the capsaicin receptor, TRPV1, by targeting the outer pore domain
    Christopher J Bohlen
    Department of Physiology, University of California, San Francisco, San Francisco, CA 94143 2140, USA
    Cell 141:834-45. 2010
    ....
  92. ncbi Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a pregnancy
    R A Anderson
    Division of Reproduction and Developmental Science, Queen s Medical Research Institute, Centre for Reproductive Biology, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK
    Reproduction 138:629-37. 2009
    ..Cumulus was isolated from 674 oocytes from 75 women undergoing ICSI and gene expression analysed by quantitative RT-PCR...
  93. pmc MicroRNA activity is suppressed in mouse oocytes
    Jun Ma
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
    Curr Biol 20:265-70. 2010
    ..small RNAs in the miRNA and RNA interference (RNAi) pathways, is essential for meiotic maturation of mouse oocytes. We found that 3'UTRs of transcripts upregulated in Dicer1(-/-) oocytes are not enriched in miRNA binding sites, ..
  94. ncbi Association between human oocyte developmental competence and expression levels of some cumulus genes
    Fabiana Cillo
    Laboratory of Biomedical Embryology, Centre for Stem Cells Research, University of Milan, Via Celoria 10, 20133 Milan, Italy
    Reproduction 134:645-50. 2007
    ..A retrospective study was performed on cumulus cells isolated from 90 oocytes retrieved from 45 patients...
  95. ncbi SYCP2 and SYCP3 are required for cohesin core integrity at diplotene but not for centromere cohesion at the first meiotic division
    Anna Kouznetsova
    Department of Cell and Molecular Biology and Center for Genomics and Bioinformatics, Karolinska Institutet, Stockholm, 171 77, Sweden
    J Cell Sci 118:2271-8. 2005
    ..This finding supports a model in which the removal of bulk cohesin from paired sister chromatids at late prophase in both meiotic and mitotic cells ensures proper chromosome compaction and segregation...
  96. ncbi Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis
    Akira Nakamura
    Laboratory for Germline Development, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Dev Cell 6:69-78. 2004
    ..These results suggest that translational repression of osk RNA is achieved through a 5'/3' interaction mediated by an eIF4E-Cup-Bru complex...
  97. ncbi R-spondin1 plays an essential role in ovarian development through positively regulating Wnt-4 signaling
    Kazuma Tomizuka
    Discovery Research Laboratories, Research Division, Kirin Pharma Co, Ltd, Takasaki shi, Gunma, 370 1295, Japan
    Hum Mol Genet 17:1278-91. 2008
    ..XX mice displayed masculinized features including pseudohermaphroditism in genital ducts, depletion of fetal oocytes, male-specific coelomic vessel formation and ectopic testosterone production in the ovaries...
  98. ncbi Differentiation potential of germ line stem cells derived from the postnatal mouse ovary
    Jason Pacchiarotti
    PrimeGen Biotech, Irvine, CA 92618, USA
    Differentiation 79:159-70. 2010
    ..belief in reproductive biology is that in most mammals female germ line stem cells are differentiated to primary oocytes during fetal development and oogenesis starts from a pool of primordial follicles after birth...
  99. pmc Mutations in the K+ channel signature sequence
    L Heginbotham
    Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115
    Biophys J 66:1061-7. 1994
    ..Furthermore, the results illustrate that the hydroxyl groups at the third and fourth positions, and the aromatic group at position seven, are not essential in determining K+ selectivity...
  100. ncbi Regulation of APC/C activity in oocytes by a Bub1-dependent spindle assembly checkpoint
    Barry E McGuinness
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, United Kingdom
    Curr Biol 19:369-80. 2009
    ..assembly checkpoint (SAC) that controls the anaphase-promoting complex or cyclosome (APC/C) operates effectively in oocytes. Experimental approaches hitherto used to inactivate the SAC in oocytes suffer from a number of drawbacks.
  101. ncbi Marine fish egg hydration is aquaporin-mediated
    Mercedes Fabra
    Center of Aquaculture Institut de Recerca i Tecnologia Agroalimentaries, Tarragona, Spain, and Reference Center in Aquaculture, Barcelona, Spain
    Science 307:545. 2005
    ....

Research Grants74

  1. Genome-wide recombination as a risk factor for nondisjunction
    NATASHA D HOLLIS; Fiscal Year: 2010
    ..In this application, the candidate proposes to examine genome-wide recombination among oocytes with non-disjoined chromosome 21...
  2. Structure and function of urea transporters
    Ming Zhou; Fiscal Year: 2013
    ..We have successfully expressed dvUT in Xenopus laevis oocytes, and found that it mediates urea flux through oocyte membrane...
  3. Mechanisms of Sex Determination in Zebrafish
    JOHN HARVEY POSTLETHWAIT; Fiscal Year: 2013
    ..develops as a bipotential organ, with a few primordial germ cells in all juveniles initially developing as oocytes. In some individuals, oocytes die and the gonad becomes a testis;in others, oocytes survive and the gonad becomes ..
  4. Intracellular Phase Diagram Creation Using a Micro-Differential Scanning Calorime
    GARY L SOLBREKKEN; Fiscal Year: 2010
    ..demonstrate the capabilities of the new measurement tool by measuring the intracellular phase diagram of mouse oocytes, mouse and rat preimplantation embryos, and zebrafish embryos...
  5. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2011
    ..FKBP6, NEK1 and cohesin proteins directly in mouse spermatocytes, and compare this directly with the action in oocytes, to determine any sexual dimorphism in the meiotic phenotype and (2) to assess the serine/threonine and tyrosine ..
  6. Efficacy of GABAA a5 receptor inverse agonists in learning impaired rats
    EARL MICHAEL GIBBS; Fiscal Year: 2010
    ..Recent in vitro studies in oocytes have shown that PWZ-029 has up to 60-fold more functional selectivity for the 15 subunit compared to 11, 12 and ..
  7. Analysis of the role of the VRK1 protein kinase in mammalian fertility
    Paula Traktman; Fiscal Year: 2011
    ..We therefore hypothesize that, within oocytes, VRK1 may be most critical for the resumption and completion of meiosis...
  8. Improving Epigenetic-based Cell Reprogramming with Proteasome Inhibition
    Jaroslaw Staszkiewicz; Fiscal Year: 2011
    ..highly efficient clinically- and commercially-relevant reprogrammed pluripotent stem cells (RePSCTM) for autologous cell replacement therapies without the use of exogenous genes, viral vectors, nuclear transfer, oocytes, or embryos.
  9. Role of murine induced pluripotent stem cells on the correction of cardiac and sk
    Diego Fraidenraich; Fiscal Year: 2010
    ..use of ES cells to treat human disease is clouded by ethical concerns surrounding the destruction of human oocytes or fertilized embryos...
  10. Design and Synthesis of Anxioselective Anxiolytics
    James M Cook; Fiscal Year: 2010
    ..poor affinity at the a1 receptor subtype of the BzR/GABAergic system and has a near perfect efficacy profile in oocytes [alpha1beta3gamma2 (no efficacy), alpha2beta3gamma2 (full agonist), alpha3beta3gamma2 (50% agonist), ..
  11. Cooperative Multicenter Reproductive Medicine Network (U10)
    MARCELLE IVONNE CEDARS; Fiscal Year: 2013
    ..Older infertility patients appear to have abnormal mitochondrial activity in oocytes and reduced production of ATP, which limits normal oocyte chromosomal disjunction and embryo development...
  12. Transposons in Female Reproductive Health and Disease
    Zev Williams; Fiscal Year: 2013
    ..assay design and computational biology with the overarching goal of elucidating the effect of transposons on oocytes and fertility...
  13. Functional Analysis of the Dual Specificity Kinase NEK1 in Mammalian Meiosis
    JOANNA KIM HOLLOWAY; Fiscal Year: 2013
    ..FKBP6, NEK1 and cohesin proteins directly in mouse spermatocytes, and compare this directly with the action in oocytes, to determine any sexual dimorphism in the meiotic phenotype and (2) to assess the serine/threonine and tyrosine ..
  14. Regulation Of Inorganic Phosphorus (Pi) Metabolism By Estrogen
    Hassane Amlal; Fiscal Year: 2013
    ..will examine the expression and transport activity of NaPi-IIa using proximal tubule suspensions, Xenopus Laevis oocytes and MDCK cells...
  15. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..The first two approaches are limited by extremely low efficiency and the requirement for fresh human oocytes or formation of somatic/embryonic stem (ES) hybrid cells, thereby limiting their further clinical applications...
  16. Aquaporin-2 (AQP2) Regulation by AMP-activated kinase(AMPK) in the Kidney Collec
    Mohammad Al-Bataineh; Fiscal Year: 2012
    ..spectrometry and the role of those residues on the subcellular localization and activity of this water channel in oocytes and mpkCCDc14 cells...
  17. Mechanisms of ENaC Inhibition by Replicating Influenza Virus: Role of M2 Protein
    Sadis Matalon; Fiscal Year: 2012
    ..acids of the influenza strain A/Udorn/72 M2 proton (H+) channel responsible for ENaC down-regulation in Xenopus oocytes microinjected with 1-,2-, and 3-ENaC...
  18. The Role of Cks Proteins in Mammalian Meiosis
    Charles H Spruck; Fiscal Year: 2010
    ..CKS2-/- male and female mice are sterile due to an arrest of spermatocytes and oocytes in metaphase of meiosis I (Ml)...
  19. CELL CYCLE CONTROL IN EARLY DROSOPHILA DEVELOPMENT
    Terry L Orr-Weaver; Fiscal Year: 2013
    ..in the developmental control of meiosis, and a genomics screen will identify mRNAs translationally activated as oocytes progress into meiosis and complete meiosis...
  20. Molecular Analysis of Juvenile Hormone Action
    Subba R Palli; Fiscal Year: 2013
    ..from ecdysteroids regulates vitellogenin gene expression in the fat body and vitellogenin uptake by the oocytes. These two systems provide a unique opportunity to study both genomic (fat body) and non-genomic (ovary) actions ..
  21. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    ..Recent studies have shown an inhibitory effect of WNK4 on NCC activity and surface expression of NCC in Xenopus oocytes, further demonstrating that WNK4 kinase regulates NCC function through direct or indirect mechanisms...
  22. WNT and gonadotropin mediated regulation of ovarian steroidogenesis
    JENNIFER HERNANDEZ GIFFORD; Fiscal Year: 2010
    ..Wnt4 have sex-reversed ovaries, express genes associated with testes development and exhibit a reduced number of oocytes at birth...
  23. Leukemia Inhibitory Factor As a Mediator of Primate Ovulation &Oocyte Maturation
    Jon D Hennebold; Fiscal Year: 2013
    ..encoding both cell surface LIF binding proteins (LIF receptor, or LIFR;gp130) were further localized to isolated oocytes and granulosa cells...
  24. Oocyte-derived R-spondin2 as a Follicle Stimulating Hormone
    AARON JW HSUEH; Fiscal Year: 2013
    ..on bioinformatic and in situ hybridization analyses, we demonstrated the exclusive expression of R-spondin2 in oocytes of primary and more advanced follicles but not in oocytes of dormant primordial follicles...
  25. Heme trafficking and its impact on systemic iron homeostasis
    Janis L Abkowitz; Fiscal Year: 2010
    ..it exports heme to heme-binding proteins, such as hemopexin and albumin, using mutagenesis analyses, Xenopus oocytes studies, and electron crystallography...
  26. Physiology and Biophysics of Cardiac Slo2.1 Channels
    Alexey V Zaitsev; Fiscal Year: 2012
    ..In Aim1 we will use site-directed mutagenesis, heterologous expression in Xenopus oocytes and voltage clamp techniques to substantiate these initial findings and definitively describe the molecular basis ..
  27. CONTROL OF MEIOSIS AND GERMLINE PROLIFERATION
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2012
    ..many species, intercellular signaling plays a pivotal role in coordinating meiosis and fertilization: developing oocytes arrest at diakinesis for prolonged periods and resume meiosis (meiotic maturation) in response to hormones...
  28. Heme trafficking and its impact on systemic iron homeostasis
    Janis L Abkowitz; Fiscal Year: 2013
    ..it exports heme to heme-binding proteins, such as hemopexin and albumin, using mutagenesis analyses, Xenopus oocytes studies, and electron crystallography...
  29. Role of nuclear receptors in origin and differentiation of spermatozoa
    ADAM MICHAEL REITZEL; Fiscal Year: 2011
    ..where undifferentiated spermatogonia are differentiated into mature spermatozoa that are then able to fertilize oocytes. In mammals, this process involves hundreds of genes that are differentially regulated in spermatogenic tissue at ..
  30. Role of Fetal Steroids in Formation and Activation of Bovine Ovarian Follicles
    Joanne E Fortune; Fiscal Year: 2010
    ..life and since this stockpile of follicles is used through the female's reproductive life as a source of oocytes for ovulation, it is critical for reproductive success and also a potential target for fertility enhancement or ..
  31. Mitochondrial Gene Therapy
    Shoukhrat M Mitalipov; Fiscal Year: 2013
    ..e., spindle transfer (ST) in mature metaphase II (MII) oocytes without interfering with subsequent nucleo-mtDNA compatibility and developmental competence...
  32. Targeting of alpha7 nAChR for therapeutic effects
    Roger L Papke; Fiscal Year: 2013
    ..Wild-type and mutant receptors will be expressed in either Xenopus oocytes or transfected mammalian cells, and we will study ion channel properties by measuring both whole-cell and single-..
  33. Regulation and Function of Germline RNP Granules
    JENNIFER SCHISA; Fiscal Year: 2010
    ..Caenorhabditis as an in vivo, multicellular model system, with the hypothesis that large RNP granules form in oocytes in order to maintain RNA stability, repress translation, and maintain overall oocyte viability/ fertility...
  34. Genetics of Meiosis and Recombination in Mice
    John C Schimenti; Fiscal Year: 2013
    ..found that both programmed and induced DSBs trigger CHK2-dependent phosphorylation of p63 (TRP63) in diplotene oocytes. These and other data establish CHK2 as essential for DNA damage surveillance in female meiosis, and we propose ..
  35. DEVELOPMENT OF MAMMALIAN OOCYTE-GRANULOSA CELL COMPLEX
    John J Eppig; Fiscal Year: 2012
    The coordination of oocyte and follicular development are essential for the development of oocytes, oocyte maturation, and timely ovulation in a condition optimal for fertilization subsequent embryogenesis...
  36. Structural Rearrangements in GABA-A Receptors
    CYNTHIA M CZAJKOWSKI; Fiscal Year: 2012
    ..We will use fluorescence recording of site-specific labels in GABAARs expressed in Xenopus oocytes to study the structural rearrangements underlying activation, desensitization, and drug modulation as they occur ..
  37. Meiotic spindle formation in Drosophila females
    Kim S McKim; Fiscal Year: 2010
    ..Drosophila melanogaster oocytes are an excellent system to elucidate the mechanisms of acentrosomal spindle formation...
  38. Development of Superactive Analogs of FSH for Human Infertility
    BRUCE DALE WEINTRAUB; Fiscal Year: 2013
    ..in vitro and in vivo rodent bioassay models including the classic ovarian weight response as well as those of oocytes, blastocysts and newborn pups resulting from embryo transfer...
  39. Divalent metal-ion transporter DMT1 and its role in intestinal metal-ion uptake
    Bryan Mackenzie; Fiscal Year: 2012
    ..through DMT1 using the voltage clamp, radiotracer (55Fe) assays, and fluorescence approaches in Xenopus oocytes expressing wildtype DMT1 or mutant proteins...
  40. GENETIC STUDIES OF ODDD
    Ethylin Wang Jabs; Fiscal Year: 2010
    ..Cellular transfection studies, utilizing mammalian cells and Xenopus oocytes, and creation of mutant GJA1 transgenic mice with alterations analogous to the mutations found in patients will ..
  41. Structure-Function Studies of the Proton-Gated Urea Channel from H. Pylori
    Hartmut Luecke; Fiscal Year: 2013
    ..hexamer to disrupt protomer association, followed by activity measurements of the monomeric state using Xenopus oocytes and proteoliposomes. 4...
  42. Development of a5* nAChR positive allosteric modulators for tobacco dependence
    JON MARTIN LINDSTROM; Fiscal Year: 2013
    ..a secondary screening strategy, we will assess the effects of novel PAMs on voltage-clamp recordings in Xenopus oocytes expressing a5* nAChRs. Oocyte will provide important insights into the mode of action of PAMs...
  43. Control of Oocyte Maturation in C. elegans
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2013
    ..elegans. In sexually reproducing animals, oocytes arrest at diplotene or diakinesis and resume meiosis (meiotic maturation) in response to hormones...
  44. EPITHELIAL OXALATE AND CITRATE TRANSPORT
    DAVID BRUCE MOUNT; Fiscal Year: 2011
    ..Direct measurement of oxalate transport in Xenopus oocytes has revealed that several other SLC26 and SLC4 exchangers are capable of oxalate transport, indicating that there ..
  45. Novel Ca receptor signaling pathways for control of renal ion transport
    Richard Tyler Miller; Fiscal Year: 2012
    ..yeast two-hybrid assays and HEK-293 cells for the basic biochemical and electrophysiologic analysis, and Xenopus oocytes for ion-specific measurement of the ClC-Kb and Kir4.1 channel activity measurements. Aim 3...
  46. Mechanism of action of novel GluN2C NMDA receptor potentiators
    Alpa Khatri; Fiscal Year: 2013
    ..loss of function chimeras will be constructed between the GluN2A and Glun2C subunits expressed in Xenopus laevis oocytes. This technique was successfully used in the lab to identify the structural determinates for a GluN2C/D ..
  47. Use of a Fragile X premutation knock-in mouse to study FXPOI
    Joshua Johnson; Fiscal Year: 2013
    ..This was associated with a smaller than normal number of granulosa cells (GCs). In addition, PM oocytes showed abnormal nuclear morphology, reduced levels of gap junction proteins, abnormal staining of their zona ..
  48. Homolog orientation and segregation in acentrosomal meiosis
    Kim S McKim; Fiscal Year: 2013
    ..proposal, we will investigate the mechanisms of homolog orientation in the acentrosomal spindle of Drosophila oocytes. The CPC is recruited to chromosomes even in the absence of microtubules but unlike mitotic cells, CPC proteins ..
  49. Ovarian-specific transcription networks regulated by the TFIID subunit TAF4b
    RICHARD NEIL FREIMAN; Fiscal Year: 2013
    ..b>Oocytes are recruited from a finite pool of primordial follicles that are usually exhausted from the ovary during mid-..
  50. Derivation of Mature Oocytes from Human Primordial Follicles
    AARON JW HSUEH; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Derivation of mature oocytes from human primordial follicles The majority of ovarian follicles stay dormant at the primordial stage for decades in women and the depletion of this dormant pool leads ..
  51. Ex Vivo Female Reproductive Tract Integration In a 3D Microphysiologic System
    Teresa K Woodruff; Fiscal Year: 2013
    ..female reproductive tract is responsible for producing endocrine hormones, developing mature, healthy gametes (oocytes) and providing the site for fertilization and an environment that supports fetal development...
  52. Modulation of the Epithelial Sodium Channel by Proteases and Peptide Inhibitors
    CHRISTOPHER JOHN PASSERO; Fiscal Year: 2010
    ..Deletions that result in enhanced ENaC activity in Xenopus oocytes will identify putative inhibitory regions...
  53. Epigenetic Regulation of Imprinting in Mouse Embryo
    Marisa S Bartolomei; Fiscal Year: 2012
    ..new information regarding the mechanism by which genomic imprinting is established and maintained in mouse oocytes and embryos...
  54. A Novel Function of the Oocyte in Estrogen-Regulated FSH Action
    Shunichi Shimasaki; Fiscal Year: 2009
    ..We have also found that the synergistic action of estrogen and oocytes occurs at a site downstream of the FSH receptor and upstream of adenylate cyclase...
  55. ANALYSIS OF REPRODUCTIVE FUNCTION USING TRANSGENIC MICE
    Martin M Matzuk; Fiscal Year: 2013
    ..Whereas mammalian oocytes were initially postulated to be passengers rather than drivers in ovarian folliculogenesis, we demonstrated that ..
  56. STUDIES ON OVARIAN RING CANALS IN DROSOPHILA
    Lynn Cooley; Fiscal Year: 2009
    ..best characterized so far in Drosophila females where ring canals allow the flow of cytoplasm from nurse cells to oocytes throughout oogenesis...
  57. Volatile Anesthetic Regulation of TASK Tandem Pore Potassium Channels
    Joseph F Cotten; Fiscal Year: 2012
    ..To test our hypothesis we will use two-electrode voltage clamp of Xenopus oocytes or Ussing Chamber studies of Fischer Rat Thyroid cells heterologously expressing TASK-3 channels and ml or m3 ..
  58. Proton-coupled folate/antifolate transport
    ISRAEL DAVID GOLDMAN; Fiscal Year: 2013
    ..transport kinetics in model human cell lines and current induction and acidification in voltage-clamped Xenopus oocytes. Results are considered with the context of an evolving structural model for PCFT in which the substrate ..
  59. Oocyte polarity and mRNA localization in Zebrafish
    FLORENCE LOUISE MARLOW; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Asymmetry in oocytes is a well-documented and conserved feature among animals including vertebrates and humans...
  60. Age and molecular mechanisms contributing to aneuploidy in oocytes
    Richard M Schultz; Fiscal Year: 2013
    ..Using mouse as a model system and imaging of live individual oocytes, Specific Aim 1 will test the hypothesis that the robustness of the SAC in oocytes decreases with age ...
  61. Regulation of meiotic recombination and chromosome segregation in mammals
    KAREN MICHELE BERKOWITZ; Fiscal Year: 2013
    ..females are subfertile, homologues separately prematurely during meiosis I, and progression of Chtf18-null oocytes through metaphase II is impaired...
  62. Antigen Identification in a Novel Model of Spontaneous Autoimmune Ovarian Disease
    Mickie H Cheng; Fiscal Year: 2012
    Autoimmune ovarian disease (AOD) is a poorly understood clinical entity wherein immune-mediated destruction of oocytes leads to premature ovarian failure and infertility...
  63. MONONUCLEAR LEUKOCYTES AND IMMUNITY
    Samuel Silverstein; Fiscal Year: 2003
    ..The project has three aims: #1. To identify by expression cloning in Xenopus oocytes the plasma membrane receptors on mononuclear phagocytes for UTP and for ATP...
  64. Sperm DNA damage in fertilization
    MONIKA WARD; Fiscal Year: 2006
    ..subjected to mechanical insults (freeze drying and freezing without cryoprotection), and then injected into the oocytes, the paternal chromosomes in the zygotes are broken...
  65. CHROMOSOME PAIRING AND NONDISJUNCTION IN HUMAN MEIOSIS
    Edith Cheng; Fiscal Year: 2000
    ..the entire germ cell developmental period, will be used to establish normative data for chromosomal anomalies in oocytes throughout reproductive life and has the potential to distinguish among the various hypotheses that have been ..
  66. Basonuclin and Ribosome Biogenesis in Mouse Oocyte and Embryo
    Richard M Schultz; Fiscal Year: 2010
    ..Based on these results, we hypothesize that oocytes transcribe several types of rRNA, some of which are used for oogenesis, whereas others for translating maternal ..
  67. Comprehensive aneuploidy diagnosis in single cells
    Nury Steuerwald; Fiscal Year: 2005
    ..It is now known that >30% of human oocytes and >50% of human preimplantation embryos are aneuploid...
  68. Novel Microcapillary Technology for the Cryopreservation of Human Oocytes
    Mehmet Toner; Fiscal Year: 2010
    ..robust, simple, easy to use, microfluidics quartz capillary freezing device, for the handling and manipulation of oocytes for ultrarapid vitrification...
  69. SAGE Analysis of Murine Oocyte Maturation
    CHARLOTTE FARIN; Fiscal Year: 2004
    ..Identification of mechanisms for the resumption of meiosis in oocytes will facilitate the development of strategies to improve the effectiveness of assisted reproduction techniques by ..
  70. APOPTOSIS AND REPRODUCTIVE AGING OF THE FEMALE
    Jonathan Tilly; Fiscal Year: 2003
    ..It is now known that apoptosis in both oocytes and GC is regulated by a genetic program conserved through evolution, with the pro-apoptotic protein, Bax, ..
  71. FAS AND BC1-2 AND GRANULOSA CELL APOPTOSIS
    Nicholas Cataldo; Fiscal Year: 2000
    ..Research plan. During embryonic and fetal development, the number of oocytes in the human ovary increases until mid-gestation, after which it steadily declines until the menopause...
  72. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2002
    ..and develop manipulation strategies which ultimately will result in efficient in vitro maturation (IVM) of human oocytes with the production of developmentally competent embryos...
  73. Factors affecting ice formation in cells and their relevance to cryopreservation
    Peter Mazur; Fiscal Year: 2011
    ..The main concern of the first four years of this grant was to determine details of IIF in oocytes of mice and Xenopus and in yeast...
  74. REGULATION OF MAMMALIAN OOCYTE DEVELOPMENT
    John Eppig; Fiscal Year: 1993
    ..this proposal focus on specific molecules that could regulate levels of cAMP and the responses to cAMP in mouse oocytes. Hypoxanthine is the principal low molecular weight substance found in porcine and murine ovarian follicular ..