endoderm

Summary

Summary: The inner of the three germ layers of the embryo.

Top Publications

  1. ncbi Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo
    Evert Kroon
    Novocell, Inc, 3550 General Atomics Ct, San Diego, California 92121, USA
    Nat Biotechnol 26:443-52. 2008
  2. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
  3. ncbi Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart
    Chen Leng Cai
    Institute of Molecular Medicine, Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Dev Cell 5:877-89. 2003
  4. pmc Generation and regeneration of cells of the liver and pancreas
    Kenneth S Zaret
    Epigenetics and Progenitor Cells Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    Science 322:1490-4. 2008
  5. ncbi Efficient differentiation of human embryonic stem cells to definitive endoderm
    Kevin A D'Amour
    CyThera Inc, 3550 General Atomics Ct, San Diego, California 92121, USA
    Nat Biotechnol 23:1534-41. 2005
  6. ncbi Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells
    Christine Mummery
    Hubrecht Laboratory, University Medical Center Utrecht, Utrecht, The Netherlands
    Circulation 107:2733-40. 2003
  7. ncbi Development of definitive endoderm from embryonic stem cells in culture
    Atsushi Kubo
    The Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 131:1651-62. 2004
  8. pmc FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse
    Minjung Kang
    Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Development 140:267-79. 2013
  9. ncbi Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling
    Stephen Frankenberg
    GReD INSERM U931 CNRS UMR6247 Clermont University, 28 Place Dunant, 63001 Clermont Ferrand, France
    Dev Cell 21:1005-13. 2011
  10. ncbi Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs
    Si Wan Kim
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Dev Biol 357:492-504. 2011

Detail Information

Publications337 found, 100 shown here

  1. ncbi Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo
    Evert Kroon
    Novocell, Inc, 3550 General Atomics Ct, San Diego, California 92121, USA
    Nat Biotechnol 26:443-52. 2008
    ..Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after ..
  2. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..population and that cell position in the ICM leads to the formation of two lineages, epiblast (EPI) and primitive endoderm (PE), by E4.5. Here, however, we show that the ICM at E3.5 is already heterogeneous...
  3. ncbi Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart
    Chen Leng Cai
    Institute of Molecular Medicine, Department of Medicine, University of California San Diego, La Jolla, CA 92093, USA
    Dev Cell 5:877-89. 2003
    ..Our results have implications for the development of specific cardiac lineages, left-right asymmetry, cardiac evolution, and isolation of cardiac progenitor cells...
  4. pmc Generation and regeneration of cells of the liver and pancreas
    Kenneth S Zaret
    Epigenetics and Progenitor Cells Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    Science 322:1490-4. 2008
    Liver and pancreas progenitors develop from endoderm cells in the embryonic foregut. Shortly after their specification, liver and pancreas progenitors rapidly acquire markedly different cellular functions and regenerative capacities...
  5. ncbi Efficient differentiation of human embryonic stem cells to definitive endoderm
    Kevin A D'Amour
    CyThera Inc, 3550 General Atomics Ct, San Diego, California 92121, USA
    Nat Biotechnol 23:1534-41. 2005
    ..Of great interest are organs derived from definitive endoderm, such as the pancreas...
  6. ncbi Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells
    Christine Mummery
    Hubrecht Laboratory, University Medical Center Utrecht, Utrecht, The Netherlands
    Circulation 107:2733-40. 2003
    ..Here, we induced cardiomyocyte differentiation of hES cells by a novel method and compared their electrophysiological properties and coupling with those of primary human fetal cardiomyocytes...
  7. ncbi Development of definitive endoderm from embryonic stem cells in culture
    Atsushi Kubo
    The Carl C Icahn Center for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Development 131:1651-62. 2004
    The cellular and molecular events regulating the induction and tissue-specific differentiation of endoderm are central to our understanding of the development and function of many organ systems...
  8. pmc FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse
    Minjung Kang
    Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Development 140:267-79. 2013
    The emergence of pluripotent epiblast (EPI) and primitive endoderm (PrE) lineages within the inner cell mass (ICM) of the mouse blastocyst involves initial co-expression of lineage-associated markers followed by mutual exclusion and salt-..
  9. ncbi Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling
    Stephen Frankenberg
    GReD INSERM U931 CNRS UMR6247 Clermont University, 28 Place Dunant, 63001 Clermont Ferrand, France
    Dev Cell 21:1005-13. 2011
    ..development, the inner cell mass (ICM) differentiates into two cell lineages--the epiblast and the primitive endoderm (PrE)--whose precursors are identifiable by reciprocal expression of Nanog and Gata6, respectively...
  10. ncbi Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs
    Si Wan Kim
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Dev Biol 357:492-504. 2011
    ..b>Endoderm commitment is controlled by the TGF-β superfamily member, Nodal, which utilizes the transcription factors, SMAD2/..
  11. ncbi Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
    Janet Rossant
    Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
    Development 136:701-13. 2009
    ..of the three lineages of the blastocyst, to the determination of the origin and the fates of the visceral endoderm in the peri-implantation mouse embryo, and to the generation of cellular and molecular activities that accompany ..
  12. pmc Pluripotency factors regulate definitive endoderm specification through eomesodermin
    Adrian Kee Keong Teo
    Laboratory for Regenerative Medicine, University of Cambridge, Cambridge, UK
    Genes Dev 25:238-50. 2011
    ..Here, we used human embryonic stem cells and mouse epiblast stem cells to study specification of definitive endoderm in vitro...
  13. ncbi FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Development 137:715-24. 2010
    Primitive endoderm (PE) and epiblast (EPI) are two lineages derived from the inner cell mass (ICM) of the E3.5 blastocyst...
  14. ncbi Nervous systems of the sea anemone Nematostella vectensis are generated by ectoderm and endoderm and shaped by distinct mechanisms
    Nagayasu Nakanishi
    Sars Centre for Marine Molecular Biology, University of Bergen, Thormoehlensgt 55, 5008 Bergen, Norway
    Development 139:347-57. 2012
    ..In contrast to bilaterians, neurons are also generated throughout the endoderm during planula stages...
  15. pmc Differential plasticity of epiblast and primitive endoderm precursors within the ICM of the early mouse embryo
    Joanna B Grabarek
    Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
    Development 139:129-39. 2012
    ..mammalian development involves the formation of two extra-embryonic lineages: trophoblast and primitive endoderm (PrE)...
  16. pmc Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm
    Irène Aksoy
    Stem Cell and Developmental Biology, Genome Institute of Singapore, Singapore
    EMBO J 32:938-53. 2013
    ..This work provides insights in understanding cell fate transcriptional regulation by highlighting the direct link between the DNA sequence of an enhancer and a developmental outcome...
  17. pmc Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation
    N Smyth
    Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool L69 3GE, United Kingdom
    J Cell Biol 144:151-60. 1999
    ..5 post coitum. These embryos lacked basement membranes and although the blastocysts had expanded, primitive endoderm cells remained in the inner cell mass, and the parietal yolk sac did not develop...
  18. pmc Oct4 cell-autonomously promotes primitive endoderm development in the mouse blastocyst
    Tristan Frum
    Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Dev Cell 25:610-22. 2013
    ..Rather, Oct4 is first required for development of the primitive endoderm (PE), an extraembryonic lineage...
  19. ncbi Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed
    Amanda B McLean
    Department of Biochemistry and Molecular Biology and Paul D Coverdell Center for Biomedical and Health Sciences, University of Georgia, Athens, Georgia 30602, USA
    Stem Cells 25:29-38. 2007
    ..Inhibition of PI3K signaling efficiently promotes differentiation of hESCs into mesendoderm and then definitive endoderm (DE) by allowing them to be specified by Activin/Nodal signals present in hESC cultures...
  20. pmc Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
    Berenika Plusa
    Developmental Biology Program, Sloan Kettering Institute, New York, USA
    Development 135:3081-91. 2008
    ..cell population during mammalian development are the extraembryonic trophectoderm (TE) and the primitive endoderm (PrE)...
  21. pmc Myc represses primitive endoderm differentiation in pluripotent stem cells
    Keriayn N Smith
    Department of Biochemistry and Molecular Biology, Paul D Coverdell Center for Biomedical and Health Sciences, The University of Georgia, 500 D W Brooks Drive, Athens, GA 30602, USA
    Cell Stem Cell 7:343-54. 2010
    ..that codeletion of c- and N-MYC in iPSCs and ESCs results in their spontaneous differentiation to primitive endoderm. We show that Myc sustains pluripotency through repression of the primitive endoderm master regulator GATA6, ..
  22. ncbi Efficient differentiation of functional hepatocytes from human embryonic stem cells
    Sadhana Agarwal
    Advanced Cell Technology, 381 Plantation Street, Worcester, Massachusetts 01605, USA
    Stem Cells 26:1117-27. 2008
    ..Highly enriched populations of definitive endoderm were generated from hESCs and then induced to differentiate along the hepatic lineage by the sequential addition ..
  23. pmc A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3
    Heather K Bone
    Centre for Regenerative Medicine and Department of Pharmacy and Pharmacology, University of Bath, Bath BA27AY, UK
    J Cell Sci 124:1992-2000. 2011
    ..Here, we demonstrate a novel route for chemically directed differentiation of human ESCs (hESCs) into definitive endoderm (DE) exploiting a selective small-molecule inhibitor of glycogen synthase kinase 3 (GSK-3)...
  24. pmc GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo
    E E Morrisey
    Department of Medicine, University of Chicago, Chicago, Illinois 60637 USA
    Genes Dev 12:3579-90. 1998
    ..Differentiated embryoid bodies derived from GATA6(-/-) ES cells lack a covering layer of visceral endoderm and severely attenuate, or fail to express, genes encoding early and late endodermal markers, including HNF4, ..
  25. pmc Histone H3K27me3 demethylases KDM6A and KDM6B modulate definitive endoderm differentiation from human ESCs by regulating WNT signaling pathway
    Wei Jiang
    Howard Hughes Medical Institute, Chevy Chase, Maryland 20815 6789, USA
    Cell Res 23:122-30. 2013
    Definitive endoderm differentiation is crucial for generating respiratory and gastrointestinal organs including pancreas and liver. However, whether epigenetic regulation contributes to this process is unknown...
  26. ncbi Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation
    Takamasa Mizoguchi
    Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1 3 1, Higashi Hiroshima, Hiroshima, Japan
    Development 135:2521-9. 2008
    ..We further found, using the endoderm-specific transgenic line Tg(sox17:EGFP), that Sdf1/Cxcr4 signaling regulates both the formation and orientation ..
  27. pmc A gene regulatory network controlling the embryonic specification of endoderm
    Isabelle S Peter
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Nature 474:635-9. 2011
    Specification of endoderm is the prerequisite for gut formation in the embryogenesis of bilaterian organisms...
  28. pmc Generation of functional human hepatic endoderm from human induced pluripotent stem cells
    Gareth J Sullivan
    Medical Research Council MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, UK
    Hepatology 51:329-35. 2010
    ..When coupled with direct differentiation to a defined lineage, such as hepatic endoderm (HE), iPSCs would revolutionize the way we study human liver biology and generate efficient "off the shelf" ..
  29. ncbi Wnt signaling in the intestinal epithelium: from endoderm to cancer
    Alex Gregorieff
    Netherlands Institute for Developmental Biology, Hubrecht Laboratory, 3584 CT Utrecht, The Netherlands
    Genes Dev 19:877-90. 2005
    ..In this review, we outline current understanding of the physiological role of Wnt signaling in intestinal biology. From this perspective, we then describe how mutational subversion of the Wnt cascade leads to colorectal cancer...
  30. ncbi casanova plays an early and essential role in endoderm formation in zebrafish
    J Alexander
    Programs in Developmental Biology and Human Genetics, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94143 0448, USA
    Dev Biol 215:343-57. 1999
    The cellular and molecular mechanisms that regulate endoderm development in vertebrates have only recently begun to be explored. Here we show that the zebrafish locus casanova plays an early and essential role in this process...
  31. pmc Differentiation of embryonic stem cells is induced by GATA factors
    Junji Fujikura
    Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Osaka 565 0871, Japan
    Genes Dev 16:784-9. 2002
    Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis...
  32. ncbi Molecular genetics of axis formation in zebrafish
    Alexander F Schier
    Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016 6497, USA
    Annu Rev Genet 39:561-613. 2005
    ..Noncanonical Wnt signaling is required for the morphogenetic movements during gastrulation. We review how the coordinated interplay of these molecules determines the fate and movement of embryonic cells...
  33. pmc Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut
    Ashley M Goss
    Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Cell 17:290-8. 2009
    ..However, the molecular pathways specifying foregut endoderm progenitors are poorly understood...
  34. ncbi Deletion of beta 1 integrins in mice results in inner cell mass failure and peri-implantation lethality
    L E Stephens
    Department of Stomatology, University of California, San Francisco 94143 0512, USA
    Genes Dev 9:1883-95. 1995
    ..Laminin-positive endoderm cells were detected in the inner cell mass area, but endoderm morphogenesis and migration were defective. By E5...
  35. pmc Vertebrate endoderm development and organ formation
    Aaron M Zorn
    Division of Developmental Biology, Cincinnati Children s Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Annu Rev Cell Dev Biol 25:221-51. 2009
    The endoderm germ layer contributes to the respiratory and gastrointestinal tracts and to all of their associated organs...
  36. ncbi Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells
    Cheryle A Séguin
    The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON M5G 1L7, Canada
    Cell Stem Cell 3:182-95. 2008
    In this study, we explore endoderm cell fate regulation through the expression of lineage-determining transcription factors...
  37. ncbi Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo
    H Sasaki
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232
    Development 118:47-59. 1993
    ..beta is expressed in the node, notochord, floor plate and gut, while HNF-3 alpha is mainly in the definitive endoderm and gut, but also in the floor plate of the midbrain...
  38. ncbi Mammalian Grb2 regulates multiple steps in embryonic development and malignant transformation
    A M Cheng
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Cell 95:793-803. 1998
    ..These data provide genetic evidence for a mammalian Grb2-Ras signaling pathway, mediated by SH2/SH3 domain interactions, that has multiple functions in embryogenesis and cancer...
  39. ncbi Differentiation of human embryonic stem cells to neural lineages in adherent culture by blocking bone morphogenetic protein signaling
    Lesley Gerrard
    Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian, EH25 9PS, UK
    Stem Cells 23:1234-41. 2005
    ..Our findings will provide a platform for studying the molecular mechanism controlling neural differentiation...
  40. pmc Nanog-like regulates endoderm formation through the Mxtx2-Nodal pathway
    Cong Xu
    Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 22:625-38. 2012
    ..the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation...
  41. ncbi The visceral yolk sac endoderm provides for absorption of nutrients to the embryo during neurulation
    Irene E Zohn
    Center for Neuroscience Research, Children s Research Institute, Children s National Medical Center, Washington, DC 20010, USA
    Birth Defects Res A Clin Mol Teratol 88:593-600. 2010
    ..During neurulation, nutrients are absorbed by histotrophic mechanisms and absorbed by endocytosis in the endoderm-derived cell layer of the visceral yolk sac (VYS)...
  42. ncbi Involvement of Ras in extraembryonic endoderm differentiation of embryonic stem cells
    Urara Yoshida-Koide
    Department of Stem Cell Biology, Graduate School of Medical Science, Kanazawa University, 13 1 Takara machi, Kanazawa, Ishikawa 920 8640, Japan
    Biochem Biophys Res Commun 313:475-81. 2004
    ..EB differentiation, a dominant-negative mutant of Ras suppressed induction of marker genes for extraembryonic endoderm differentiation, including GATA-4, GATA-6, alpha-fetoprotein, and hepatocyte nuclear factor 3beta, while an ..
  43. pmc The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation
    Apriliana E R Kartikasari
    Department of Medicine, University of California Los Angeles, Los Angeles, CA 90095 7073, USA
    EMBO J 32:1393-408. 2013
    ..Our results demonstrate that Jmjd3 sequentially associates with two T-box factors, Tbx3 and Eomes to drive stem cell differentiation towards the definitive endoderm lineage.
  44. pmc Wnt6 activates endoderm in the sea urchin gene regulatory network
    Jenifer Croce
    Biologie du Développement, Observatoire Oceanologique de Villefranche sur Mer, UPMC, UMR7009 CNRS, 06230 Villefranche sur Mer, France
    Development 138:3297-306. 2011
    ..If that cortical region of the egg is removed, addition of Wnt6 rescues endoderm. At a molecular level, the vegetal cortex region contains a localized concentration of Dishevelled (Dsh) protein, ..
  45. pmc Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo
    Daniel W Stuckey
    Molecular Embryology Group, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, UK
    Development 138:1521-30. 2011
    ..In the mouse embryo, migration of the anterior visceral endoderm (AVE) to the prospective anterior establishes the anterior-posterior (A-P) axis...
  46. ncbi Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo
    Katsuyoshi Takaoka
    Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, and CREST, Japan Science and Technology Corporation, 1 3Yamada oka, Suita, Osaka 565 0871, Japan
    Nat Cell Biol 13:743-52. 2011
    Anterior-posterior polarity of the mouse embryo has been thought to be established when distal visceral endoderm (DVE) at embryonic day (E) 5.5 migrates toward the future anterior side to form anterior visceral endoderm (AVE)...
  47. ncbi Regionalisation of the endoderm progenitors and morphogenesis of the gut portals of the mouse embryo
    Vanessa Franklin
    Embryology Unit, Children s Medical Research Institute, University of Sydney, Locked Bag 23, Wentworthville, NSW 2145, Australia
    Mech Dev 125:587-600. 2008
    ..This fate-mapping study reveals that the progenitors of all major parts of the embryonic gut are already present in endoderm of the early-head-fold to early-somite stage (1-9 somites) mouse embryo...
  48. pmc Functional heterogeneity of embryonic stem cells revealed through translational amplification of an early endodermal transcript
    Maurice A Canham
    Institute for Stem Cell Research, Medical Research Council Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
    PLoS Biol 8:e1000379. 2010
    ..Here we use a transgenic allele designed to detect low level transcription in the primitive endoderm lineage as a tool to identify an immediate early endoderm-like ES cell state...
  49. ncbi Definitive endoderm of the mouse embryo: formation, cell fates, and morphogenetic function
    Samara L Lewis
    Embryology Unit, Children s Medical Research Institute, University of Sydney, Sydney, NSW, Australia
    Dev Dyn 235:2315-29. 2006
    The endoderm is one of the primary germ layers but, in comparison to ectoderm and mesoderm, has received less attention. The definitive endoderm forms during gastrulation and replaces the extraembryonic visceral endoderm...
  50. pmc Multiple roles for Med12 in vertebrate endoderm development
    Chong Hyun Shin
    Department of Biochemistry and Biophysics, Liver Center, University of California, San Francisco, CA 94158, USA
    Dev Biol 317:467-79. 2008
    In zebrafish, the endoderm originates at the blastula stage from the most marginal blastomeres...
  51. ncbi Generation of insulin-producing islet-like clusters from human embryonic stem cells
    Jianjie Jiang
    Cell Therapy Research, Geron Corporation, Menlo Park, CA 94025, USA
    Stem Cells 25:1940-53. 2007
    ..In this 36-day protocol, hESCs were treated with sodium butyrate and activin A to generate definitive endoderm coexpressing CXCR4 and Sox17, and CXCR4 and Foxa2...
  52. ncbi In vitro derivation of functional insulin-producing cells from human embryonic stem cells
    Wei Jiang
    Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 17:333-44. 2007
    ..Activin A was used in the initial stage to induce definitive endoderm differentiation from human ES cells, as detected by the expression of the definitive endoderm markers Sox17 and ..
  53. ncbi Beta-catenin signaling marks the prospective site of primitive streak formation in the mouse embryo
    Othman A Mohamed
    Department of Biology, McGill University Health Center, Royal Victoria Hospital, Montreal, QC, Canada
    Dev Dyn 231:416-24. 2004
    ..form of beta-catenin as well as beta-catenin signaling is first detectable in the extraembryonic visceral endoderm in day 5.5 embryos. Before the initiation of gastrulation at day 6...
  54. pmc A computational model for understanding stem cell, trophectoderm and endoderm lineage determination
    Vijay Chickarmane
    Division of Biology, California Institute of Technology, Pasadena, California, United States of America
    PLoS ONE 3:e3478. 2008
    ..determines the trophectoderm lineage whereas an excess of Gata-6 over Nanog determines differentiation into the endoderm lineage...
  55. ncbi Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells
    Malgorzata Borowiak
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell Stem Cell 4:348-58. 2009
    ..These compounds induce nearly 80% of ESCs to form definitive endoderm, a higher efficiency than that achieved by Activin A or Nodal, commonly used protein inducers of endoderm...
  56. ncbi Vertebrate endoderm development
    J M Wells
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Cell Dev Biol 15:393-410. 1999
    b>Endoderm, one of the three principal germ layers, contributes to all organs of the alimentary tract...
  57. ncbi Sequence-specific regulator Prdm14 safeguards mouse ESCs from entering extraembryonic endoderm fates
    Ziyang Ma
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California, USA
    Nat Struct Mol Biol 18:120-7. 2011
    ..Here, we show that Prdm14 safeguards mouse ESC (mESC) maintenance by preventing induction of extraembryonic endoderm (ExEn) fates. Conversely, Prdm14 overexpression impairs ExEn differentiation during embryoid body formation...
  58. ncbi Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula
    J A Belo
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of California, Los Angeles 90095 1662, USA
    Mech Dev 68:45-57. 1997
    ..cer-l), a gene encoding a novel secreted protein that is specifically expressed in the anterior visceral endoderm during early gastrulation...
  59. ncbi Origins of bilateral symmetry: Hox and dpp expression in a sea anemone
    John R Finnerty
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Science 304:1335-7. 2004
    ..These data suggest that bilateral symmetry arose before the evolutionary split of Cnidaria and Bilateria...
  60. ncbi Growth-limiting role of endothelial cells in endoderm development
    Fredrik Wolfhagen Sand
    Stem Cell and Pancreas Developmental Biology, Stem Cell Center, Department of Laboratory Medicine, Lund, Lund University, BMC B10 Klinikgatan 26, SE 221 84 Lund, Sweden
    Dev Biol 352:267-77. 2011
    b>Endoderm development is dependent on inductive signals from different structures in close vicinity, including the notochord, lateral plate mesoderm and endothelial cells...
  61. ncbi Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture
    Shinsuke Tada
    Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Development 132:4363-74. 2005
    Bipotent mesendoderm that can give rise to both endoderm and mesoderm is an established entity from C. elegans to zebrafish...
  62. ncbi Activin/Nodal signaling controls divergent transcriptional networks in human embryonic stem cells and in endoderm progenitors
    Stephanie Brown
    The Anne McLaren Laboratory for Regenerative Medicine, Department of Surgery, University of Cambridge, Cambridge, United Kingdom
    Stem Cells 29:1176-85. 2011
    ..to maintain pluripotency of human embryonic stem cells (hESCs) and to induce their differentiation toward endoderm. However, the mechanisms by which Activin/Nodal signaling achieves these opposite functions remain unclear...
  63. ncbi Activin and BMP4 synergistically promote formation of definitive endoderm in human embryonic stem cells
    Adrian K K Teo
    Institute of Medical Biology, Agency for Science, Technology and Research, 06 06 Immunos, Singapore 138648
    Stem Cells 30:631-42. 2012
    ..Numerous reports demonstrate their differentiation into definitive endoderm (DE) cells, the germ layer from which pancreatic β cells and hepatocytes arise, solely from exposure to a high ..
  64. pmc Cell adhesive affinity does not dictate primitive endoderm segregation and positioning during murine embryoid body formation
    Robert Moore
    Department of Medicine, Sylvester Comprehensive Cancer Center, Department of Obstetrics and Gynecology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    Genesis 47:579-89. 2009
    ..have reappraised these issues by implementing embryoid bodies to model the patterning of epiblast and primitive endoderm layers...
  65. pmc Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells
    Micha Drukker
    Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA
    Nat Biotechnol 30:531-42. 2012
    ..Subsets detected in self-renewing cultures, including CXCR4(+) cells, expressed primitive endoderm genes. Expression of Cxcr4 in primitive endoderm was confirmed in visceral endoderm of mouse embryos...
  66. pmc Efficient and directive generation of two distinct endoderm lineages from human ESCs and iPSCs by differentiation stage-specific SOX17 transduction
    Kazuo Takayama
    Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan
    PLoS ONE 6:e21780. 2011
    ..HMG box 17 (SOX17) overexpression in ESCs leads to differentiation of either extraembryonic or definitive endoderm cells, respectively, the mechanism of these distinct results remains unknown...
  67. ncbi Lineage tracing of the endoderm during oral development
    Michaela Rothova
    Department of Craniofacial Development and Stem Cell Biology, and Department of Orthodontics, King s College London, Guy s Hospital, London Bridge, London, United Kingdom
    Dev Dyn 241:1183-91. 2012
    The contribution of the endoderm to the oral tissues of the head has been debated for many years...
  68. pmc Targeted disruption of cubilin reveals essential developmental roles in the structure and function of endoderm and in somite formation
    Brian T Smith
    Department of Cell Biology and Anatomy Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    BMC Dev Biol 6:30. 2006
    Cubilin is a peripheral membrane protein that interacts with the integral membrane proteins megalin and amnionless to mediate ligand endocytosis by absorptive epithelia such as the extraembryonic visceral endoderm (VE).
  69. ncbi An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish
    Wen Fang Tseng
    Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan Town, Miaoli County 350, Taiwan, ROC
    Dev Biol 357:541-57. 2011
    ..Here we comprehensively demonstrate that a pan-deuterostome endoderm specification kernel exists in zebrafish...
  70. pmc mTOR supports long-term self-renewal and suppresses mesoderm and endoderm activities of human embryonic stem cells
    Jiaxi Zhou
    Department of Cell and Developmental Biology and Institute for Genomic Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 106:7840-5. 2009
    ..Inhibition of mTOR impairs pluripotency, prevents cell proliferation, and enhances mesoderm and endoderm activities in hESCs...
  71. ncbi The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis
    D Dufort
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, M5G 1X5
    Development 125:3015-25. 1998
    During early embryogenesis, the transcription factor HNF3beta is expressed in visceral and definitive endoderm, node, notochord and floorplate...
  72. pmc Anterior visceral endoderm directs ventral morphogenesis and placement of head and heart via BMP2 expression
    Mary Madabhushi
    Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA
    Dev Cell 21:907-19. 2011
    ..BMP2 expressed in anterior visceral endoderm (AVE) signals to epiblast derivatives during gastrulation to orchestrate initial stages of ventral morphogenesis, ..
  73. pmc Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish
    Bettina Reichenbach
    Department of Developmental Biology, University of Freiburg, Biology I, Hauptstrasse 1, 79104 Freiburg, Germany
    Dev Biol 318:52-64. 2008
    ..We have investigated the roles of endoderm and Sonic hedgehog (SHH) in the development of the ENS...
  74. pmc Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells
    Fangfang Lai
    Department of Cell Biology, University of Miami School of Medicine, 1011 NW 15th St, Miami, FL 33136, USA
    Development 139:1476-86. 2012
    ..Lineage tracing and TUNEL staining reveal that Nanos1-deficient PGCs fail to migrate out of the endoderm. They appear to undergo apoptosis rather than convert to normal endoderm...
  75. ncbi (beta)-catenin mediates the specification of endoderm cells in ascidian embryos
    K Imai
    Department of Zoology, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Development 127:3009-20. 2000
    ..accumulated in the nuclei of vegetal blastomeres, suggesting that it plays a role in the specification of endoderm. (2) Mis- and/or overexpression of (beta)-catenin induced the development of an endoderm-specific alkaline ..
  76. pmc Direct transcriptional regulation of Gata4 during early endoderm specification is controlled by FoxA2 binding to an intronic enhancer
    Anabel Rojas
    Centro Andaluz de Biología Molecular y Medicina Regenerativa CIBERDEM, Sevilla, Spain
    Dev Biol 346:346-55. 2010
    The embryonic endoderm is a multipotent progenitor cell population that gives rise to the epithelia of the digestive and respiratory tracts, the liver and the pancreas...
  77. ncbi Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos
    W S Chen
    Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021
    Genes Dev 8:2466-77. 1994
    ..of HNF-4, a transcription factor in the steroid hormone receptor superfamily, is detected only in the visceral endoderm of mouse embryos during gastrulation and is expressed in certain embryonic tissues from 8.5 days of gestation...
  78. ncbi Hepatic specification of the gut endoderm in vitro: cell signaling and transcriptional control
    R Gualdi
    Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Genes Dev 10:1670-82. 1996
    We have studied the initial development of pluripotent gut endoderm to hepatocytes using a tissue explant system from mouse embryos...
  79. ncbi A genomic regulatory network for development
    Eric H Davidson
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Science 295:1669-78. 2002
    ..A gene regulatory network that controls the specification of endoderm and mesoderm in the sea urchin embryo is summarized here...
  80. pmc Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm
    Pei Wang
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell Stem Cell 8:335-46. 2011
    ..stem cells (hESCs) can provide insights into development of inaccessible human tissues such as embryonic endoderm. Progress in this area has been hindered by a lack of methods for isolating endodermal cells and tracing fates of ..
  81. pmc Tissue-specific regulation of mouse microRNA genes in endoderm-derived tissues
    Yan Gao
    Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6145, USA
    Nucleic Acids Res 39:454-63. 2011
    ..Here, we determine the entire microRNAome of three endoderm-derived tissues, liver, jejunum and pancreas, using ultra-high throughput sequencing...
  82. pmc Distinct mesodermal signals, including BMPs from the septum transversum mesenchyme, are required in combination for hepatogenesis from the endoderm
    J M Rossi
    Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Genes Dev 15:1998-2009. 2001
    Mesodermal signaling is critical for patterning the embryonic endoderm into different tissue domains...
  83. pmc Proposal of a model of mammalian neural induction
    Ariel J Levine
    Laboratory of Molecular Vertebrate Embryology, The Rockefeller University, New York, NY 10021, USA
    Dev Biol 308:247-56. 2007
    ..The anterior visceral endoderm protects the pre-specified anterior neural fate from similar posteriorization, allowing formation of forebrain...
  84. ncbi The zebrafish van gogh mutation disrupts tbx1, which is involved in the DiGeorge deletion syndrome in humans
    Tatjana Piotrowski
    National Institutes of Health, NICHD, LMG, Bldg 6B, 9000 Rockville Pike, Bethesda, MD 20892, USA
    Development 130:5043-52. 2003
    ..Furthermore, we provide evidence for regulatory interactions between vgo/tbx1 and edn1 and hand2, genes that are implicated in the control of pharyngeal arch development and in the etiology of DGS...
  85. ncbi PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum
    M F Offield
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 2175, USA
    Development 122:983-95. 1996
    ..These results offer additional insight into the role of pdx-1 in the determination and differentiation of the posterior foregut, particularly regarding the proliferation and differentiation of the pancreatic progenitors...
  86. ncbi Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer
    T Bouwmeester
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, 90095 1737, USA
    Nature 382:595-601. 1996
    ..The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo.
  87. pmc Chemokine signaling controls endodermal migration during zebrafish gastrulation
    Sreelaja Nair
    Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA
    Science 322:89-92. 2008
    ..Anterior migration of the mesoderm and endoderm has largely been interpreted to result from epiboly and convergent-extension movements that drive body elongation...
  88. pmc Tissue entrainment by feedback regulation of insulin gene expression in the endoderm of Caenorhabditis elegans
    Coleen T Murphy
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 104:19046-50. 2007
    ..Our findings show that feedback regulation of insulin gene expression coordinates DAF-16 activity among the tissues, and they establish the intestine, which is the animal's entire endoderm, as an important insulin-signaling center.
  89. ncbi BMP-4 is required for hepatic specification of mouse embryonic stem cell-derived definitive endoderm
    Valerie Gouon-Evans
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, New York 10029, USA
    Nat Biotechnol 24:1402-11. 2006
    ..in the presence of activin A in serum-free conditions, mouse embryonic stem cells efficiently generate an endoderm progenitor population defined by the coexpression of either Brachyury, Foxa2 and c-Kit, or c-Kit and Cxcr4...
  90. pmc Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and noncanonical Wnt11 signaling
    Yan Li
    Cincinnati Children s Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Genes Dev 22:3050-63. 2008
    ..is expressed in the surface cells of the foregut epithelium, whereas wnt11 is expressed in the underlying deep endoderm. Depletion of Sfrp5 results in reduced foregut gene expression and hypoplastic liver and ventral pancreatic buds...
  91. ncbi Nanog is required for primitive endoderm formation through a non-cell autonomous mechanism
    Daniel M Messerschmidt
    Department of Molecular Embryology, Max Planck Institute for Immunobiology, Stuebeweg 51, 79108 Freiburg, Germany
    Dev Biol 344:129-37. 2010
    ..In a second lineage decision ICM cells segregate into Epiblast (EPI) and primitive endoderm (PE). EPI and PE formation depend on the activity of the transcription factors Nanog and Gata4/6...
  92. ncbi The role of the zebrafish nodal-related genes squint and cyclops in patterning of mesendoderm
    Scott T Dougan
    Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Development 130:1837-51. 2003
    Nodal signals, a subclass of the TGFbeta superfamily of secreted factors, induce formation of mesoderm and endoderm in vertebrate embryos...
  93. ncbi Anterior definitive endoderm from ESCs reveals a role for FGF signaling
    Gillian M Morrison
    MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Cell Stem Cell 3:402-15. 2008
    ..biology is limited by an inability to isolate in vitro equivalents of regionally specified anterior definitive endoderm (ADE)...
  94. pmc Dynamic signaling network for the specification of embryonic pancreas and liver progenitors
    Ewa Wandzioch
    Cell and Developmental Biology Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    Science 324:1707-10. 2009
    ..These findings may enhance progenitor cell specification from stem cells for biomedical purposes and can help explain incomplete programming in stem cell differentiation protocols...
  95. pmc Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytes
    Hua Liu
    Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hepatology 51:1810-9. 2010
    ..So far, there has been no report of endoderm-derived human iPS cells, and this has prevented comprehensive comparative investigations of the quality of human ..
  96. ncbi Hepatocyte-like cells derived from human embryonic stem cells specifically via definitive endoderm and a progenitor stage
    Gabriella Brolén
    Cellartis AB, Arvid Wallgrens Backe 20, 41346 Göteborg, Sweden
    J Biotechnol 145:284-94. 2010
    ..The first phase involves the formation of definitive endoderm. Next, these cells are treated with factors known to promote the induction and proliferation towards hepatic ..
  97. ncbi Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse
    Sebastian J Arnold
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Development 135:501-11. 2008
    ..and chimera studies demonstrate for the first time that Eomes is required for specification of the definitive endoderm lineage...
  98. pmc β-catenin specifies the endomesoderm and defines the posterior organizer of the hemichordate Saccoglossus kowalevskii
    Sébastien Darras
    Institut de Biologie du Developpement de Marseille Luminy, UMR 6216, CNRS, Universite de la Mediterranee, Campus de Luminy, Marseille Cedex 09, France
    Development 138:959-70. 2011
    ..Although the specification of vegetal territories (endoderm) by β-catenin has been demonstrated in distantly related animals (cnidarians, a protostome, echinoderms and ..
  99. ncbi Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos
    Aditya J Sethi
    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA
    Science 335:590-3. 2012
    The segregation of embryonic endomesoderm into separate endoderm and mesoderm fates is not well understood in deuterostomes...
  100. ncbi Essential role for the homeoprotein vHNF1/HNF1beta in visceral endoderm differentiation
    C Coffinier
    Unite des Virus Oncogenes, CNRS URA 1644, and Unité de Biologie du Développement, CNRS URA 1960, Institut Pasteur, F 75724 Paris Cedex 15, France
    Development 126:4785-94. 1999
    ..this late requirement, vHNF1 may play earlier roles during development, as it is first expressed in the visceral endoderm at the onset of gastrulation...
  101. ncbi The Danforth's short tail mutation acts cell autonomously in notochord cells and ventral hindgut endoderm
    R Maatman
    The Jackson Laboratory, Bar Harbor, ME 04609, USA
    Development 124:4019-28. 1997
    ..Sd leads to the degeneration of notochord cells and the number or allocation of notochord precursors from the tail bud to the notochordal plate seems impaired, whereas notochord formation from the node appears to be unaffected...

Research Grants70

  1. BMP signaling in vertebrate development
    Ken W Y Cho; Fiscal Year: 2013
    ..reveal that BMP signaling is not only active in the mesoderm and ectoderm, as is well known, but also in the endoderm 3...
  2. Use of umbilical cord as a unique stem cell source for tissue regeneration
    Xiao Dong Chen; Fiscal Year: 2013
    ..including osteoblasts, adipocytes, chondroblasts and cardiomyocytes (mesoderm), nerve (ectoderm) and hepatocytes (endoderm)...
  3. Stem Cells For Myocardial Repair: A Large Bore Magnet Study
    Jianyi Zhang; Fiscal Year: 2012
    ..the single cell level into cells with phenotypic and functional characteristics of mesoderm, neuroectoderm, and endoderm lineages...
  4. Regulation of Apical-Basal Cell polarity during Intestinal Epithelial Morphogenes
    Nan Gao; Fiscal Year: 2013
    ..My previous Ph.D. and postdoctoral training experience has been continuously related to gut endoderm development...
  5. Morphogenesis of mammalian gut endoderm
    Anna Katerina Hadjantonakis; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): The gut endoderm gives rise to the major cell types of many internal organs, including the thyroid, thymus, lung, stomach, liver, pancreas, intestine and bladder...
  6. Stem cell-mediated reversal of thymic involution in premature aging models
    Kenneth I Weinberg; Fiscal Year: 2010
    ..We have been able to derive functional TEC from murine embryonic stem cells (mESC). Definitive endoderm was generated by Activin A stimulation of mESC, and the cells were then lentivirally transduced with the Tbx1 ..
  7. Role of TGF-? Signaling in Embryonic Stem Cell Maintenance and Endodermal Differe
    Alan C Mullen; Fiscal Year: 2013
    ..to maintain human embryonic stem cells and is also required to direct embryonic stem cells to differentiate into endoderm, which will give rise to the cells of the liver, gut and pancreas...
  8. Role of the Bradykinin Pathway in Craniofacial Development
    LAURA ANNE JACOX; Fiscal Year: 2013
    ..The mouth develops from the "extreme anterior domain" (EAD), where the ectoderm and endoderm directly juxtapose...
  9. Control of Cardiac Lineage Commitment and Differentiation in Murine Development
    Ibrahim J Domian; Fiscal Year: 2013
    ..aims: 1) Determine if miR200 is promotes FHF development by supporting mesoderm differentiation and suppressing endoderm and ectoderm differentiation;2) Determine the mechanisms by which miR200 controls FHF development;3) Determine ..
  10. Transcriptional Control of Liver Development
    Joshua Friedman; Fiscal Year: 2009
    DESCRIPTION (provided by applicant): The liver develops from cells of the ventral foregut endoderm which differentiate into heptoblasts and proliferate into the adjacent mesenchyme to form the liver bud...
  11. TSHR and thyrocyte development
    Reigh Yi Lin; Fiscal Year: 2013
    ..In this application, we will expand on human ES cell studies focused on the mechanisms regulating endoderm induction and its specification to thyroid lineage during ES cell differentiation...
  12. Avian Middle Ear Development
    SUSAN CAROLINE CHAPMAN; Fiscal Year: 2012
    ..ossification of the columella using histology and in situ hybridization analysis, 2) determine if the pharyngeal endoderm, lying adjacent to the site of columella condensation is crucial for induction of the columella condensation by ..
  13. Neural Crest Modulates FGF Signaling in the Pharynx
    Margaret Loewy Kirby; Fiscal Year: 2010
    ..We will electroporate an FGF8b expressing plasmid into the pharyngeal endoderm of chick embryos in ovo to correlate developmental events in the secondary heart field with outflow alignment...
  14. Uncovering the transcription factor networks in early human cell specification
    ALEXANDER MINCHEV TSANKOV; Fiscal Year: 2013
    ..During gastrulation, pluripotent epiblast cells give rise to the three germ layers-- endoderm, mesoderm, and ectoderm...
  15. DNA Methyltrasferase Gene Expression in Colon Cancer
    Stephen B Baylin; Fiscal Year: 2013
    ..It encodes a protein essential for gut endoderm differentiation which inhibits, in the nucleus, Wnt pathway activation of [unreadable]-catenin mediated ..
  16. Genetic Pathways Directing Ventral Folding Morphogenesis in Mammalian Embryos
    ELIZABETH H LACY; Fiscal Year: 2013
    ..The proposed studies build directly on our recent finding that the Anterior Visceral Endoderm (AVE), a well defined signaling center in the pre-streak (PS) and Early Streak (ES) mouse embryo, plays a central ..
  17. Epitenetic regulation and enhancement of beta cell differentiation
    Arthur D Riggs; Fiscal Year: 2011
    ..In spite of the recent progress in deriving early definitive and pancreatic endoderm-like progenitors from hESCs, a robust generation of later functional pancreatic beta-like cells has not yet been ..
  18. Defining the genetic program of primordial lung progenitors
    LAERTIS OIKONOMOU; Fiscal Year: 2013
    ..FGF and Wnt ligands are known to induce lung fate within definitive endoderm in vivo and we will assess whether they have a similar function in vitro...
  19. Allogeneic Placental Stem Cells for Tissue Repair and Regeneration
    Vijay Kumar; Fiscal Year: 2010
    ..mesenchymal stem cells (MSC), with the ability to differentiate to all three germ layers (ectoderm, endoderm, and mesoderm) in vivo...
  20. Extrinsic signaling in neural crest and craniofacial development
    KIMBERLY ELAINE INMAN; Fiscal Year: 2010
    ..migration and differentiation with extrinsic patterning cues derived from the surrounding mesoderm, ectoderm and endoderm. We propose that the cranial mesoderm and the vascular network it generates are instrumental in patterning neural ..
  21. Ethanol-induced Lower Jaw Loss in Bmp Signaling Pathway Mutants
    CHARLES BENJAMIN LOVELY; Fiscal Year: 2013
    ..causes strikingly similar defects to Meckel's cartilage due to disrupted morphogenesis of the anterior endoderm and subsequent defects to the oral ectoderm...
  22. THE ROLE OF GATA6 IN LUNG DEVELOPMENT
    Edward E Morrisey; Fiscal Year: 2013
    ..1. Genetic deletion of any of these factors significantly impairs lung endoderm differentiation and in some cases postnatal repair and regeneration...
  23. 9th International Congress of Vertebrate Morphology: Jaw Development Symposium
    Richard A Schneider; Fiscal Year: 2010
    ..changes in transcription factor regulation as well as expression of secreted molecules by the pharyngeal endoderm underlie mandibular arch patterning. Dr...
  24. Bmp-signaling in craniofacial development
    JAMES FRANCIS MARTIN; Fiscal Year: 2012
    ..Recent work also indicates that signaling from pharyngeal endoderm is important for early mandibular morphogenesis...
  25. Estrogen Regulation of Hepatic Growth
    Wolfram Goessling; Fiscal Year: 2013
    ..In Specific Aim 1, we seek to define the role, timing, and targets of estrogen signaling during endoderm specification and liver formation;these studies will make use of both chemical and genetic modification of ..
  26. Role of FGF10 in liver organogenesis and alcohol-impaired regeneration
    KASPER SAONUN WANG; Fiscal Year: 2010
    ..FgflO, expressed in the embryonic foregut mesoderm, normally interacts with the adjacent endoderm through Fibroblast Growth Factor Receptor 2 1Mb (FGFR2b)...
  27. Single Cell/RNA-Seq dissection of Human iPS cell development into intestine
    James M Wells; Fiscal Year: 2013
    ..Global transcriptomes will be determined for the starting human pluripotent stem cells, the induced endoderm, the primitive gut tube, and developing intestinal organoids...
  28. TRANS-ACTING FACTORS CAUSING CELL SPECIFIC GENE CONTROL
    Kenneth S Zaret; Fiscal Year: 2013
    ..As a model system, we investigate the specification of liver progenitors from multipotent foregut endoderm cells in the early mouse embryo...
  29. KLF5 regulation of intestinal development and stem cell homeostasis.
    NOAH FREEMAN SHROYER; Fiscal Year: 2013
    ..KLF5 is highly expressed in the early embryo and the primordial endoderm during gastrulation, with continued expression in the mid/hindgut and embryonic intestine during morphogenesis ..
  30. Mechanisms regulating hepatic specification and differentiation in zebrafish
    CHONG H SHIN; Fiscal Year: 2013
    ..sic] signals such as Fibroblast growth factors (Fgfs) and Bone morphogenetic proteins (Bmps), the cultured endoderm turned on a gene, Pdx1, that normally expressed in the foregut endoderm [sic], including the stomach, duodenum ..
  31. Chromatin regulation of liver and pancreas specification
    CHENGRAN XU; Fiscal Year: 2013
    ..and use such information, along with direct perturbations of chromatin modifiers, to improve the inductions of endoderm, hepatic and beta cells from ESCs. Dr...
  32. Deciphering the gene regulatory network controlling vertebrate endodermal fates
    Aaron M Zorn; Fiscal Year: 2013
    ..So far, only a handful of transcription factors (TFs) are known to play key roles in endoderm development, and how these TFs functionally interact in a gene regulatory network (GRN) is poorly understood and ..
  33. Mechanism of MAPK Cytoplasmic Retention in Differentiation of ES Cells
    Elizabeth R Smith; Fiscal Year: 2013
    ..The majority of the ES cells differentiate into cells with properties that resemble the primitive endoderm of early embryos...
  34. Roles of hedgehog signaling in foregut development
    JOHN A KLINGENSMITH; Fiscal Year: 2013
    ..Aim 3 elucidates the role of Shh in the different patterns of stratification and differentiation within the endoderm. In Aim 4, we determine the spatiotemporal functions of Shh signaling in differentiation of specialized foregut ..
  35. Characterization of Endoderm Stem Cells Derived from Murine ESCs
    Jon S Odorico; Fiscal Year: 2010
    ..It is widely accepted that the production of these populations will first require generation of definitive endoderm. Thus, the successful derivation and stable propagation of endoderm-restricted stem cell lines would be a ..
  36. Ectoderm formation in the early Xenopus embryo
    Matthew Kofron; Fiscal Year: 2013
    ..Investigator): All tissues of the vertebrate body arise from three primary germ layers;the ectoderm, mesoderm and endoderm. The ectoderm gives rise to the central nervous system and epidermis...
  37. Transcriptional regulation of liver specification in Xenopus tropicalis
    ANDREA ELIZABETH WILLS; Fiscal Year: 2012
    ..cell fate is thought to be acquired through a sequential process of commitment: cells are first directed to an endoderm fate by extracellular Nodal signaling, then acquire liver competence through the expression of specific ..
  38. Functional analysis of Cripto in mouse embryogenesis
    Michael M Shen; Fiscal Year: 2012
    ..provided by applicant): During early mouse development, an extraembryonic cell type known as the distal visceral endoderm (DVE) plays a central role in the anterior-posterior (A-P) patterning of the embryo...
  39. Rapid Multiplexed Nanoprobe Assays for Pluripotent Stem Cell Differentiation
    David Larocca; Fiscal Year: 2013
    ..In phase I, we will develop a multiplexed PTQD assay for definitive endoderm (DE) and identify additional peptides for ectoderm and mesoderm assays...
  40. Signals from the reproductive system that regulate aging in C. elegans
    Cynthia J Kenyon; Fiscal Year: 2013
    ..In C. elegans, the intestine behaves as the entire endoderm, including the adipose tissue.)...
  41. PDX-1 Regulation of Intestinal Pattern Formation
    Eric Sibley; Fiscal Year: 2012
    ..The epithelial cells of the small intestine are derived from the gut endoderm cell lineage and function in the digestion and absorption of ingested nutrients with spatial differences in gene ..
  42. Causes of Variability in Craniofacial Disease
    Johann K Eberhart; Fiscal Year: 2013
    ..embryos have a jaw-loss phenotype similar to that in mutants that disrupt development of the anterior endoderm. Here, we determine the mechanisms for these interactions...
  43. Regionalization of the Vertebrate Endoderm
    Victoria E Prince; Fiscal Year: 2013
    ..The overall goal of this proposal is to understand how pancreatic progenitors are produced from undifferentiated endoderm during embryonic development...
  44. Rho GTPases and polarity signaling pathways in morphogenesis and migration
    Alan Hall; Fiscal Year: 2013
    ..time-lapse ex vivo imaging to show that Rac1 is required for the collective migration of anterior visceral endoderm (AVE) cells in embryonic day 5.5 mouse embryos...
  45. Signaling and Epigenetic Control of Beta Cell Development and Regeneration
    Kenneth S Zaret; Fiscal Year: 2011
    ..revealed a complex and dynamic network of signals that induce PDX1+ pancreatic progenitors from undifferentiated endoderm cells, as well as conditions in adult tissues in which beta cells can be generated from non-beta cells...
  46. Role of the Groucho/Grg/TLE corepressor protein in delaying tissue specification
    Kimberly Blahnik; Fiscal Year: 2013
    ..of the Groucho/Grg/TLE (Grg) corepressor complex, and establish its role in delaying the induction of multipotent endoderm progenitors...
  47. Understanding liver bud emergence, formation and potential
    Kimberly D Tremblay; Fiscal Year: 2013
    ..The liver bud emerges from the endoderm in a process that is typical of all endoderm-derived organs including the lung, pancreas and thyroid, using ..
  48. microRNAs and integrative control of cardiopoiesis
    Mark Mercola; Fiscal Year: 2013
    ..roles in initiating embryonic heart formation and act by distinguishing cardiac mesoderm from foregut endoderm. Here we propose to apply this same approach to identify miRs that control the next step in cardiogenesis: the ..
  49. A Mouse Model of Acrodermatitis Enteropathica
    Glen K Andrews; Fiscal Year: 2012
    ..is based on our finding that they localize to opposite membranes of polarized intestinal enterocytes and visceral endoderm cells, cell-types critical for proper zinc homeostasis, and they show opposite responses to zinc availability in ..
  50. ALBINO DELETION COMPLEX AND EARLY MOUSE DEVELOPMENT
    Terry Magnuson; Fiscal Year: 2013
    ..The epiblast precursor cells, unlike the primitive endoderm or trophectoderm, undergo imprinted XCI, then reactivation, and finally random XCI during implantation...
  51. Genetic and molecular anlaysis of neural development
    Howard Sirotkin; Fiscal Year: 2009
    ..The mesoderm and endoderm are important sources of signals that induce neural tissue and establish asymmetries within the neural plate...
  52. Mammalian Foregut and Liver Development
    Aaron M Zorn; Fiscal Year: 2012
    ..The mouse embryonic liver is induced from the ventral foregut endoderm by Fgf signals from the cardiac mesoderm...
  53. Induction of Cardiovascular Cells from hESCs by Embryonic Cues
    John W Lough; Fiscal Year: 2013
    ..This objective will be approached by adhering to an over-riding theme, based on the interplay between endoderm and mesoderm lineages during embryonic development, that specific endodermal lineages induce differentiation of ..
  54. ErbB signaling in vertebrate morphogenesis
    Chenbei Chang; Fiscal Year: 2010
    ..Gastrulation is the process through which mesoderm and endoderm are placed inside the embryos to form internal tissues and organs...
  55. Mechanisms of endoderm specification along the A-P axis.
    James M Wells; Fiscal Year: 2010
    ..Our published and preliminary studies show that endoderm patterning is highly conserved across vertebrate species and we hypothesize that that one growth factor, FGF4, ..
  56. CONTROL OF PROSTATE-SPECIFIC GENE EXPRESSION
    Robert J Matusik; Fiscal Year: 2013
    ..specific gene expression with a complex of AR (signal dependent), FOXA1 (developmental cell specific for endoderm) and USF2 (differentiation specific) reveals a remarkable coming together of regulatory factors to dictate ..
  57. Differentiating Nkx2.1-positive lung epithelial progenitors from ES cells
    Jayaraj Rajagopal; Fiscal Year: 2013
    ..The first strategy relies on the stepwise differentiation of pluripotent cells first into endoderm, then into anterior endoderm, and subsequently into Nkx2.1-positive lung progenitor cells and Nkx2...
  58. Developmental &Molecular Mechanisms Employed by Chato, KRAB Zinc Finger Protein
    María J García-García; Fiscal Year: 2013
    ..of chato mutants confirmed that chato is required for the proper rearrangement of cells that lead to definitive endoderm convergent extension. chato encodes ZFP568, a novel Kruppel-Associated-Box (KRAB) Zinc finger protein...
  59. Regulation of pancreatic islet location and size during embryonic development
    Mary D Kinkel; Fiscal Year: 2011
    ..I have also shown that Cdx4 is required in the endoderm to correctly localize the pancreas...
  60. The San Diego Epigenome Center
    Bing Ren; Fiscal Year: 2013
    ..on both undifferentiated hESC and four hESC-derived early embryonic cell lineages including extraembryonic endoderm, trophoblast, mesendoderm (a common precursor to mesodermal and endodermal lineages), and mesenchymal cells (a ..
  61. The Roles of Definitive Endoderm in Establishment of Left-Right Asymmetry
    Yukio Saijoh; Fiscal Year: 2013
    ..Here I will address these questions by investigating roles of definitive endoderm in LR determination. To date, no report has been focused on roles of endoderm in LR asymmetry...
  62. Developing a new model of spider gastrulation
    STEVEN DANIELS BLACK; Fiscal Year: 2010
    ..by tracing fluorescently labeled cells in living embryos and by in situ hybridization for molecular markers of endoderm and mesoderm. (2) Mechanism of elongation of the embryo...
  63. Studies on a Novel RNA that Promotes Heart Development
    LARRY LEMANSKI; Fiscal Year: 2006
    ..The defect can be corrected by culturing hearts with normal anterior endoderm tissue, in medium conditioned by the anterior endoderm, or in total RNA isolated from endoderm or conditioned ..
  64. THYMIC LYMPHOCYTE DEVELOPMENT
    Irving Weissman; Fiscal Year: 2004
    ..The stromal cells of the thymus that regulate this process are derived from all 3 tissue layers - endoderm, ectoderm, and mesoderm...
  65. CDX EXPRESSION IN THE GASTROINTESTINAL TRACT
    Debra Silberg; Fiscal Year: 1999
    ..two in vivo models the effect of Cdx1 and Cdx2 expression first, in early embryogenesis on undifferentiated endoderm and second, in differentiated intestine and stomach by conditional expression of the Cdx proteins...
  66. Embryonic development of taste buds.
    LINDA BARLOW; Fiscal Year: 2009
    ..Most recently, we have demonstrated that .=pithelium destined to give to taste buds, the pharyngeal endoderm, receives signals very early, during gastrulation, that initially specify, or direct the fate of, this embryonic ..
  67. CONTROL OF BETA CELL SPECIFICATION AND PROPAGATION
    Ole Madsen; Fiscal Year: 2000
    ..Thus a common, fundamental regulatory pathway is likely to operate within the developing neuro-ectoderm and endoderm to control neuronal and endocrine cell differentiation, respectively...
  68. DEVELOPMENTAL BIOLOGY OF THE IMPLANTING MAMMALIAN EMBRYO
    Roger Pedersen; Fiscal Year: 2000
    ..that mediate implantation and develop into the placenta and fetal membranes, namely, trophoblast, extraembryonic endoderm, and extraembryonic mesoderm...
  69. DEVELOPMENT OF THE GUSTATORY SYSTEM
    RICHARD NORTHCUTT; Fiscal Year: 2005
    ..These placodes are known to be induced by pharyngeal endoderm, but the time frame of their induction is not known...
  70. NOVEL PROTEIN ASSOCIATED WITH HEART DEVELOPMENT
    LARRY LEMANSKI; Fiscal Year: 2001
    ..This is corrected by coculturing mutant hearts with normal anterior endoderm, culturing them in conditioned medium from normal endoderm cultures, or culturing them in RNA isolated from ..