blastocyst

Summary

Summary: Early preimplantation mammalian EMBRYO in the post-MORULA stage. Its structure consists of a TROPHOBLASTS-enclosed fluid-filled cavity with an inner cell mass. After EMBRYO IMPLANTATION, the inner cell mass of the blastocyst becomes the embryonic disc of the developing embryo.

Top Publications

  1. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
  2. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
  3. pmc Multipotent cell lineages in early mouse development depend on SOX2 function
    Ariel A Avilion
    Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Genes Dev 17:126-40. 2003
  4. pmc Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
    G R Martin
    Proc Natl Acad Sci U S A 78:7634-8. 1981
  5. ncbi Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst
    Guoji Guo
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    Dev Cell 18:675-85. 2010
  6. pmc Nanog is the gateway to the pluripotent ground state
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Cell 138:722-37. 2009
  7. ncbi Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
  8. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
  9. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
  10. pmc Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells
    Kathryn Blaschke
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Obstetrics and Gynecology and Center for Reproductive Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Nature 500:222-6. 2013

Detail Information

Publications412 found, 100 shown here

  1. ncbi Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells
    Ian Chambers
    Institute for Stem Cell Research, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JQ, Scotland
    Cell 113:643-55. 2003
    ..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity...
  2. ncbi The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells
    Kaoru Mitsui
    Laboratory of Animal Molecular Technology, Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630 0192, Japan
    Cell 113:631-42. 2003
    ..nanog-deficient ES cells lost pluripotency and differentiated into extraembryonic endoderm lineage. These data demonstrate that Nanog is a critical factor underlying pluripotency in both ICM and ES cells...
  3. pmc Multipotent cell lineages in early mouse development depend on SOX2 function
    Ariel A Avilion
    Division of Developmental Genetics, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Genes Dev 17:126-40. 2003
    ..5 days postcoitum. Our data suggest that maternal components could be involved in establishing early cell fate decisions and that a combinatorial code, requiring SOX2 and OCT4, specifies the first three lineages present at implantation...
  4. pmc Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
    G R Martin
    Proc Natl Acad Sci U S A 78:7634-8. 1981
    ..The availability of such cell lines should made possible new approaches to the study of early mammalian development...
  5. ncbi Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst
    Guoji Guo
    Department of Biological Sciences, National University of Singapore, Singapore 117543
    Dev Cell 18:675-85. 2010
    Three distinct cell types are present within the 64-cell stage mouse blastocyst. We have investigated cellular development up to this stage using single-cell expression analysis of more than 500 cells...
  6. pmc Nanog is the gateway to the pluripotent ground state
    Jose Silva
    Wellcome Trust Centre for Stem Cell Research, University of Cambridge, Cambridge CB2 1QR, UK
    Cell 138:722-37. 2009
    ..These findings suggest that Nanog choreographs synthesis of the naive epiblast ground state in the embryo and that this function is recapitulated in the culmination of somatic cell reprogramming...
  7. ncbi Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation
    Hitoshi Niwa
    Laboratory for Pluripotent Cell Studies, RIKEN Center for Developmental Biology CDB, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Cell 123:917-29. 2005
    ..This suggests that reciprocal inhibition between lineage-specific transcription factors might be involved in the first differentiation event of mammalian development...
  8. ncbi Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway
    Claire Chazaud
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G1X5, Canada
    Dev Cell 10:615-24. 2006
    ..We propose a model in which the ICM develops as a mosaic of EPI and PE progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers...
  9. ncbi Dynamic reprogramming of DNA methylation in the early mouse embryo
    Fatima Santos
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom
    Dev Biol 241:172-82. 2002
    ..De novo methylation was observed specifically in the inner cell mass (ICM) but not in the trophectoderm of the blastocyst and hence may have an important role in early lineage specification...
  10. pmc Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells
    Kathryn Blaschke
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Obstetrics and Gynecology and Center for Reproductive Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Nature 500:222-6. 2013
    ..Collectively, the results of this study establish vitamin C as a direct regulator of Tet activity and DNA methylation fidelity in ES cells...
  11. ncbi Molecular cues to implantation
    S K Dey
    Department of Pediatrics, Vanderbilt University Medical Center, MCN D4100, Nashville, Tennessee 37232, USA
    Endocr Rev 25:341-73. 2004
    Successful implantation is the result of reciprocal interactions between the implantation-competent blastocyst and receptive uterus...
  12. ncbi Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer
    Satoshi Kishigami
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, 2 2 3 Minatojima minamimachi, Kobe 650 0047, Japan
    Biochem Biophys Res Commun 340:183-9. 2006
    ..h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells, spleen cells, neural stem cells, ..
  13. ncbi Blastocyst lineage formation, early embryonic asymmetries and axis patterning in the mouse
    Janet Rossant
    Research Institute, The Hospital for Sick Children and Departments of Molecular Genetics, and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
    Development 136:701-13. 2009
    ..been gained into the cellular and molecular events that lead to the establishment of the three lineages of the blastocyst, to the determination of the origin and the fates of the visceral endoderm in the peri-implantation mouse embryo,..
  14. pmc Germline competent embryonic stem cells derived from rat blastocysts
    Ping Li
    Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Cell 135:1299-310. 2008
    ..Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases...
  15. ncbi FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst
    Yojiro Yamanaka
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, Toronto, Ontario M5G 1X8, Canada
    Development 137:715-24. 2010
    Primitive endoderm (PE) and epiblast (EPI) are two lineages derived from the inner cell mass (ICM) of the E3.5 blastocyst. Recent studies showed that EPI and PE progenitors expressing the lineage-specific transcriptional factors Nanog and ..
  16. ncbi Multiple female reproductive failures in cyclooxygenase 2-deficient mice
    H Lim
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City 66160, USA
    Cell 91:197-208. 1997
    ....
  17. ncbi A genome-wide study of gene activity reveals developmental signaling pathways in the preimplantation mouse embryo
    Q Tian Wang
    Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
    Dev Cell 6:133-44. 2004
    ..Overall, these data provide a detailed temporal profile of gene expression that reveals the richness of signaling processes in early mammalian development...
  18. pmc Oct4 cell-autonomously promotes primitive endoderm development in the mouse blastocyst
    Tristan Frum
    Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Dev Cell 25:610-22. 2013
    ..Here, we show that neither maternal nor zygotic Oct4 is required for the formation of EPI cells in the blastocyst. Rather, Oct4 is first required for development of the primitive endoderm (PE), an extraembryonic lineage...
  19. pmc Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst
    Berenika Plusa
    Developmental Biology Program, Sloan Kettering Institute, New York, USA
    Development 135:3081-91. 2008
    ..suggests PrE precursors exhibit an apparently random distribution within the inner cell mass of the early blastocyst and then segregate to their final position lining the cavity by the late blastocyst...
  20. pmc An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Nucleic Acids Res 34:e42. 2006
    ..This study provides a strategy to analyze biophysical events in medicine as well as in neural, stem cell and developmental biology, where small numbers of distinctive or diseased cells play critical roles...
  21. ncbi Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development
    Ikuhiro Okamoto
    Mammalian Developmental Epigenetics Group, Institut Curie, CNRS UMR 3215, INSERM U934, Paris 75248, France
    Nature 472:370-4. 2011
    ..Paternal XCI is reversed in the inner cell mass of the blastocyst and random XCI subsequently occurs in epiblast cells...
  22. ncbi Roadmap to embryo implantation: clues from mouse models
    Haibin Wang
    Department of Pediatrics, Division of Reproductive and Developmental Biology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nat Rev Genet 7:185-99. 2006
    ..Synchronizing embryonic development until the blastocyst stage with the uterine differentiation that takes place to produce the receptive state is crucial to successful ..
  23. ncbi Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial
    Richard T Scott
    Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA
    Fertil Steril 100:697-703. 2013
    To determine whether blastocyst biopsy and rapid quantitative real-time polymerase chain reaction (qPCR)-based comprehensive chromosome screening (CCS) improves in vitro fertilization (IVF) implantation and delivery rates.
  24. pmc A maternal-zygotic effect gene, Zfp57, maintains both maternal and paternal imprints
    Xiajun Li
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 15:547-57. 2008
    ..The effects on genomic imprinting are consistent with the maternal-zygotic lethality of Zfp57 mutants...
  25. pmc Deterministic and stochastic allele specific gene expression in single mouse blastomeres
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, United Kingdom
    PLoS ONE 6:e21208. 2011
    ....
  26. ncbi Birth of piglets derived from porcine zygotes cultured in a chemically defined medium
    Koji Yoshioka
    Department of Production Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    Biol Reprod 66:112-9. 2002
    ..Our results indicate that porcine zygotes can develop into blastocysts in a chemically defined medium and to full term by transfer to recipients after culture...
  27. ncbi Expression of Xist during mouse development suggests a role in the initiation of X chromosome inactivation
    G F Kay
    Section of Comparative Biology, Medical Research Council Clinical Research Centre, Harrow, England
    Cell 72:171-82. 1993
    ..The imprint on Xist expression is lost shortly before gastrulation when random X inactivation occurs. Our data support a direct role for Xist in the initiation of X inactivation...
  28. ncbi Validation of microarray comparative genomic hybridization for comprehensive chromosome analysis of embryos
    Cristina Gutiérrez-Mateo
    Reprogenetics, Livingston, New Jersey, USA
    Fertil Steril 95:953-8. 2011
    ..To validate and determine the best array-comparative genomic hybridization (aCGH; array-CGH) protocols for preimplantation genetic screening (PGS)...
  29. pmc RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo- and in vitro-derived bovine blastocysts
    Ashley M Driver
    Department of Dairy Science, University of Wisconsin, Madison, WI 53706, USA
    BMC Genomics 13:118. 2012
    ..It is rare to have more than 40% of fertilized in vitro cattle oocytes reach blastocyst stage by day 8 of culture, and pregnancy rates are reported as less than 45% for in vitro produced embryos...
  30. pmc RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos
    Shogo Matoba
    RIKEN BioResource Center, Tsukuba, Ibaraki 305 0074, Japan
    Proc Natl Acad Sci U S A 108:20621-6. 2011
    ..corrected SCNT-specific aberrant Xist expression at the morula stage, but failed to do so thereafter at the blastocyst stage...
  31. pmc E-cadherin null mutant embryos fail to form a trophectoderm epithelium
    L Larue
    Max Planck Institut fur Immunbiologie, Freiburg, Germany
    Proc Natl Acad Sci U S A 91:8263-7. 1994
    ..At the blastocyst stage, E-cadherin-negative embryos failed to form a trophectodermal epithelium or a blastocyst cavity...
  32. pmc Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis
    Fuchou Tang
    Wellcome Trust Cancer Research, UK
    Cell Stem Cell 6:468-78. 2010
    ..Such genetic and epigenetic events may contribute to a switch from a normal developmental program in adult cells during the formation of diseased tissues, including cancers...
  33. ncbi Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture
    L E Young
    Roslin Institute Edinburgh, Roslin, Midlothian, Scotland, UK
    Nat Genet 27:153-4. 2001
    ..This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures...
  34. pmc Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice
    Jongdae Shin
    Program in Gene Function and Expression, University of Massachusetts Medical School UMMS, Worcester, Massachusetts 01605, USA
    Nature 467:977-81. 2010
    ..These results assign crucial functions to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI...
  35. ncbi Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings
    P Guerin
    Ecole Nationale Veterinaire de Lyon, Unité Biologie de la Reproduction, Marcy l Etoile, France
    Hum Reprod Update 7:175-89. 2001
    ..Nevertheless, maintaining the pro-oxidant-antioxidant equilibrium in embryos through such supplementation is a complex problem. Further studies are necessary to limit oxidative stress during embryo culture...
  36. ncbi Aneuploidy in the human blastocyst
    E Fragouli
    University of Oxford, Nuffield Department of Obstetrics and Gynaecology, Institute of Reproductive Sciences, Oxford, UK
    Cytogenet Genome Res 133:149-59. 2011
    ..in the last couple of years that large numbers of embryos at the final stage of preimplantation development, the blastocyst stage, 5 days after fertilization, have been subjected to detailed analysis...
  37. ncbi Temporal divergence in the pattern of messenger RNA expression in bovine embryos cultured from the zygote to blastocyst stage in vitro or in vivo
    P Lonergan
    Department of Animal Science and Production, University College Dublin, Lyons Research Farm, Newcastle, County Dublin 4, Ireland
    Biol Reprod 69:1424-31. 2003
    ..approximately 30 hpi (2-cell), 2 (4-cell), 3 (8-cell), 4 (16-cell), 5 (early morula), 6 (compact morula), or 7 (blastocyst) days post insemination...
  38. ncbi Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells
    Hui Yang
    Group of Epigenetic Reprogramming, State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Cell 149:605-17. 2012
    ..Our results demonstrate that AG-haESCs can be used as a genetically tractable fertilization agent for the production of live animals via injection into oocytes...
  39. ncbi New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system
    G Vajta
    Embryo Technology Center, Danish Institute of Agricultural Sciences, Research Centre Foulum, Tjele, Denmark
    Mol Reprod Dev 55:256-64. 2000
    ..The WOW system resulted in higher blastocyst/oocyte rates for all three modules (single: 59%; group of five: 61%; single zona-digested: 53%) than the culture ..
  40. ncbi The culture of zygotes to the blastocyst stage changes the postnatal expression of an epigentically labile allele, agouti viable yellow, in mice
    Hugh D Morgan
    Royal North Shore Hospital, Disciplines of Medicine and Physiology, University of Sydney, Sydney, 2065 New South Wales, Australia
    Biol Reprod 79:618-23. 2008
    ..These results show for the first time that the preimplantation embryo's growth environment can affect the postnatal expression of a defined epigenetically sensitive allele...
  41. ncbi Clinical application of comprehensive chromosomal screening at the blastocyst stage
    William B Schoolcraft
    Colorado Center for Reproductive Medicine, Lone Tree, Colorado, USA
    Fertil Steril 94:1700-6. 2010
    To evaluate a new strategy for comprehensive chromosome screening at the blastocyst stage.
  42. pmc Making the blastocyst: lessons from the mouse
    Katie Cockburn
    Department of Molecular Genetics, University of Toronto, Canada
    J Clin Invest 120:995-1003. 2010
    ..development, which is the period extending from fertilization to implantation, results in the formation of a blastocyst with three distinct cell lineages...
  43. ncbi Epigenetic reprogramming in mammalian development
    W Reik
    Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge CB2 4AT, UK
    Science 293:1089-93. 2001
    ..Here we look at what is known about reprogramming in mammals and discuss how it might relate to developmental potency and imprinting...
  44. ncbi Gata3 regulates trophoblast development downstream of Tead4 and in parallel to Cdx2
    Amy Ralston
    Program in Developmental and Stem Cell Biology, Hospital for Sick Children Research Institute, MARS Building, Toronto Medical Discovery Tower, Toronto, ON M5G 1L7, Canada
    Development 137:395-403. 2010
    The mouse blastocyst and stem cells derived from its tissue lineages provide a unique genetic system for examining the establishment and loss of pluripotency...
  45. pmc Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo
    Samantha A Morris
    Department of Physiology, Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    Proc Natl Acad Sci U S A 107:6364-9. 2010
    ..In conclusion, we propose a model in which the timing of cell internalization, cell position, and cell sorting combine to determine distinct lineages of the preimplantation mouse embryo...
  46. ncbi Non-invasive amino acid turnover predicts human embryo developmental capacity
    Franchesca D Houghton
    Department of Biology, University of York, PO Box 373, York YO10 5YW, UK
    Hum Reprod 17:999-1005. 2002
    ..These problems could be overcome if single embryos of known developmental competence could be selected for transfer on day 2/3 of development, but current methods, which rely on morphological appearance, are poor predictors of viability...
  47. ncbi The molecular foundations of the maternal to zygotic transition in the preimplantation embryo
    Richard M Schultz
    Department of Biology, University of Pennsylvania, 19104 6018, USA E mail
    Hum Reprod Update 8:323-31. 2002
    ....
  48. ncbi Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch
    Mary E Donohoe
    Howard Hughes Medical Institute, USA
    Mol Cell 25:43-56. 2007
    ..Yy1 and Ctcf associate through specific protein-protein interactions and together transactivate Tsix. We propose that the Ctcf-Yy1-Tsix complex functions as a key component of the X chromosome binary switch...
  49. ncbi Developmental control of the early mammalian embryo: competition among heterogeneous cells that biases cell fate
    Alexander W Bruce
    The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, United Kingdom
    Curr Opin Genet Dev 20:485-91. 2010
    ..EPI) during mammalian pre-implantation development are prerequisites for the successful implantation of the blastocyst. The mechanisms underlying these earliest stages of development remain a fertile topic for research and informed ..
  50. ncbi Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro
    J L M R Leroy
    Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Reproduction 130:485-95. 2005
    ..of PA or SA during oocyte maturation had negative effects on maturation, fertilization and cleavage rate and blastocyst yield...
  51. pmc Colony-stimulating factor 2 (CSF-2) improves development and posttransfer survival of bovine embryos produced in vitro
    Barbara Loureiro
    Department of Animal Sciences, University of Florida, Gainesville, Florida 32611 0910, USA
    Endocrinology 150:5046-54. 2009
    ..Our objective was to verify effects of CSF2 on blastocyst yield, determine posttransfer survival, and evaluate properties of the blastocyst formed after CSF2 treatment...
  52. ncbi Development and application of a chemically defined medium for the in vitro production of porcine embryos
    Koji Yoshioka
    Research Team for Production Diseases, National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    J Reprod Dev 57:9-16. 2011
    ..This paper discusses the developmental ability and normality of porcine IVP embryos, and limitations and advancements in this system...
  53. ncbi Corticotropin-releasing hormone promotes blastocyst implantation and early maternal tolerance
    A Makrigiannakis
    School of Medicine, University of Crete, Heraklion 71110, Greece
    Nat Immunol 2:1018-24. 2001
    The semi-allograft embryo in the blastocyst stage implants itself in the endometrium, yet no immune rejection processes are activated...
  54. ncbi Involvement of BCL2 family members in the regulation of human oocyte and early embryo survival and death: gene expression and beyond
    Imène Boumela
    CHU Montpellier, Institute for Research in Biotherapy, Hopital Saint Eloi, Montpellier F 34000, France
    Reproduction 141:549-61. 2011
    ..We suggest that implications of not only aberrant gene expression but also abnormal subcellular protein redistribution should be established in pathological conditions resulting in infertility...
  55. pmc Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos
    Karen Goossens
    Department of Animal Nutrition, Genetics, Breeding and Ethology, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B 9820 Merelbeke, Belgium
    BMC Dev Biol 5:27. 2005
    ..Normalization of the data using unstable controls can result in erroneous conclusions, especially when only one reference gene is used...
  56. pmc Maternal obesity promotes a proinflammatory signature in rat uterus and blastocyst
    Kartik Shankar
    Arkansas Children s Nutrition Center, 15 Children s Way, Slot 512 20B, Little Rock, Arkansas 72202, USA
    Endocrinology 152:4158-70. 2011
    ..We conducted global transcriptomic profiling in the uterus and periimplantation blastocyst, gene/protein expression analyses of inflammatory pathways in conjunction with endocrine and metabolic ..
  57. ncbi Synergistic effects of glutathione and β-mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system supplemented with L-cysteine
    Changyong Choe
    Republic of Korea Animal Genetic Resources Station, National Institute of Animal Science, RDA, Namwon, Korea
    J Reprod Dev 56:575-82. 2010
    ..Specifically, we examined the synergistic effects of antioxidants on development to the blastocyst stage in a culture system supplemented with L-cysteine during IVM...
  58. ncbi Androgenetic haploid embryonic stem cells produce live transgenic mice
    Wei Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Nature 490:407-11. 2012
    ..Our findings show the developmental pluripotency of androgenentic haploids and provide a new tool to quickly produce genetic models for recessive traits. They may also shed new light on assisted reproduction...
  59. ncbi Predictive value of cumulus cell apoptosis with regard to blastocyst development of corresponding gametes
    Claudia Maria Corn
    University of Salzburg, Institute of Zoology, Salzburg, Austria
    Fertil Steril 84:627-33. 2005
    ..To test if a high degree of apoptosis in cumulus cells could indicate diminished oocyte quality and developmental competence...
  60. ncbi Identification of ZAG1, a novel protein expressed in mouse preimplantation, and its putative roles in zygotic genome activation
    Toshiki Matsuoka
    Division of Biological Science, Graduate School of Biology Oriented Science and Technology, Kinki University, Wakayama, Japan
    J Reprod Dev 54:192-7. 2008
    ..These results suggest that ZAG1 may be closely associated with zygotic gene expression in mouse preimplantation embryos...
  61. ncbi Deposition of acetylated histones by RNAP II promoter clearance may occur at onset of zygotic gene activation in preimplantation mouse embryos
    Mikiko Tokoro
    Division of Biological Science, Graduate School of Biology Oriented Science and Technology, Kinki University, Wakayama, Japan
    J Reprod Dev 56:607-15. 2010
    ....
  62. ncbi Paternal body mass index is associated with decreased blastocyst development and reduced live birth rates following assisted reproductive technology
    Hassan W Bakos
    Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, Robinson Institute, University of Adelaide, Adelaide, Australia
    Fertil Steril 95:1700-4. 2011
    ..To determine the relationship between paternal body mass index (BMI), embryo development and pregnancy, and live birth outcomes after assisted reproductive technology (ART)...
  63. ncbi Effects of purple sweet potato anthocyanins on development and intracellular redox status of bovine preimplantation embryos exposed to heat shock
    Miki Sakatani
    National Agricultural Research Center for Kyushu Okinawa Region, Japan
    J Reprod Dev 53:605-14. 2007
    ..Embryos exposed to heat shock without anthocyanins showed a significant decrease in blastocyst formation rate and GSH content (P<0.05) and an increase in intracellular ROS (P<0...
  64. ncbi Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
    D K Gardner
    Colorado Center for Reproductive Medicine, Englewood, Colorado, USA
    Fertil Steril 73:1155-8. 2000
    To determine the relationship between blastocyst score and pregnancy outcome.
  65. ncbi Histone modifications at the blastocyst Axin1(Fu) locus mark the heritability of in vitro culture-induced epigenetic alterations in mice
    Raul Fernandez-Gonzalez
    Departamento de Reproduccion Animal, INIA, Madrid, Spain
    Biol Reprod 83:720-7. 2010
    ..at blastocysts stage and 2) the histone marks (H3K4 me2, H3K9 me3, H3K9 ac, and H4K20 me3) present at the blastocyst stage at the specific Axin1(Fu) locus...
  66. ncbi Scriptaid corrects gene expression of a few aberrantly reprogrammed transcripts in nuclear transfer pig blastocyst stage embryos
    Kristin M Whitworth
    Division of Animal Sciences, University of Missouri, E125 Animal Science Research Center, Columbia, USA
    Cell Reprogram 13:191-204. 2011
    ..BLIVV), in vitro fertilized (BLIVF), or nuclear transfer derived (NT, three different activation methods) blastocyst stage embryos and the donor cell line by microarray analysis, and to determine if treatment of reconstructed ..
  67. ncbi Selective loss of imprinting in the placenta following preimplantation development in culture
    Mellissa R W Mann
    Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Development 131:3727-35. 2004
    ..These results indicate that tissues of trophectoderm origin are unable to restore genomic imprints and suggest that mechanisms that safeguard imprinting might be more robust in the embryo than in the placenta...
  68. ncbi Autophagy is essential for preimplantation development of mouse embryos
    Satoshi Tsukamoto
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Science 321:117-20. 2008
    ..Protein synthesis rates were reduced in the autophagy-null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals...
  69. pmc Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocysts
    Atsushi Fukuda
    Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan
    PLoS ONE 5:e11274. 2010
    ..Our findings provide a framework to further understand the reprogramming in SECNT embryos...
  70. ncbi Symposium: innovative techniques in human embryo viability assessment. Assessing embryo viability by measurement of amino acid turnover
    Roger G Sturmey
    Department of Biology, University of York, York, UK
    Reprod Biomed Online 17:486-96. 2008
    ..Data on amino acid profiling of embryos from a number of species are then discussed. It is concluded that this technology has excellent potential to improve the selection of single embryos for transfer in clinical IVF...
  71. pmc Blastocysts don't go it alone. Extrinsic signals fine-tune the intrinsic developmental program of trophoblast cells
    D Randall Armant
    Department of Obstetrics and Gynecology, C S Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201 1415, USA
    Dev Biol 280:260-80. 2005
    The preimplantation embryo floats freely within the oviduct and is capable of developing into a blastocyst independently of the maternal reproductive tract...
  72. ncbi Time-lapse analysis of mouse embryo development in oxygen gradients
    P L Wale
    Department of Zoology, University of Melbourne, Parkville, VIC 3010, Australia
    Reprod Biomed Online 21:402-10. 2010
    ..Partial or complete culture in atmospheric oxygen resulted in significantly fewer blastocyst cell numbers compared with control (P<0.05)...
  73. ncbi Domains of high-polarized and low-polarized mitochondria may occur in mouse and human oocytes and early embryos
    Jonathan Van Blerkom
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Hum Reprod 17:393-406. 2002
    ..Here, we investigated whether deltapsim in oocytes and preimplantation embryos was heterogeneous and cell contact-associated...
  74. pmc Somatic nucleus reprogramming is significantly improved by m-carboxycinnamic acid bishydroxamide, a histone deacetylase inhibitor
    Xiangpeng Dai
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    J Biol Chem 285:31002-10. 2010
    ..More importantly, CBHA increased blastocyst quality compared with trichostatin A, another prevalent histone deacetylase inhibitor reported previously...
  75. ncbi Gene replacement reveals a specific role for E-cadherin in the formation of a functional trophectoderm
    Natalia G Kan
    Max Planck Institut fur Immunbiologie, Abteilung für Molekulare Embryologie, Stubeweg 51, D 79108 Freiburg, Germany
    Development 134:31-41. 2007
    ..molecule E-cadherin is ultimately necessary for the transition from compacted morula to the formation of the blastocyst to ensure correct establishment of adhesion junctions in the trophectoderm...
  76. ncbi Dynamics of global gene expression changes during mouse preimplantation development
    Toshio Hamatani
    Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Suite 3000, Baltimore, MD 21224, USA
    Dev Cell 6:117-31. 2004
    ..gene activation (MGA), precedes the dynamic morphological and functional changes from the morula to blastocyst stage...
  77. ncbi Aberrant methylation of donor genome in cloned bovine embryos
    Y K Kang
    Animal Developmental Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB, PO Box 115, Yusong, Taejon, 305-600, South Korea
    Nat Genet 28:173-7. 2001
    ..Our findings indicate that the developmental anomalies of cloned embryos could be due to incomplete epigenetic reprogramming of donor genomic DNA...
  78. ncbi Variability in the expression of trophectodermal markers beta-human chorionic gonadotrophin, human leukocyte antigen-G and pregnancy specific beta-1 glycoprotein by the human blastocyst
    A Jurisicova
    Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Toronto, Ontario, Canada
    Hum Reprod 14:1852-8. 1999
    Improved culture conditions that support the development of human embryos to the blastocyst stage in vitro led to the prospect of blastocyst transfer to increase pregnancy rates...
  79. ncbi Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage
    Connie C Wong
    Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, Stanford, California, USA
    Nat Biotechnol 28:1115-21. 2010
    ..examining a large set of zygotes from in vitro fertilization (IVF), we find that success in progression to the blastocyst stage can be predicted with >93% sensitivity and specificity by measuring three dynamic, noninvasive imaging ..
  80. ncbi Making a firm decision: multifaceted regulation of cell fate in the early mouse embryo
    Magdalena Zernicka-Goetz
    The Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK m zernicka goetz
    Nat Rev Genet 10:467-77. 2009
    ..We discuss how mechanisms proposed in previously distinct models might work in concert to progressively reinforce cell fate decisions through feedback loops...
  81. ncbi Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality
    Dimitrios Rizos
    Department of Animal Science and Production and Conway Institute for Biomedical and Biomolecular Research, University College Dublin, Lyons Research Farm, Newcastle, County Dublin, Ireland
    Mol Reprod Dev 61:234-48. 2002
    ..bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification...
  82. ncbi Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes
    M B Rogers
    Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115
    Development 113:815-24. 1991
    ..5 mouse blastocyst. It is also present in the polar trophoblast of the blastocyst...
  83. pmc Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts
    P Bermejo-Alvarez
    Departamento de Reproduccion Animal, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid 28040, Spain
    Proc Natl Acad Sci U S A 107:3394-9. 2010
    ..6, suggesting that X-Chr inactivation is partially achieved at the blastocyst stage. Almost half of the transcripts up-regulated in female embryos exhibiting more than 1...
  84. ncbi Deciphering the cross-talk of implantation: advances and challenges
    B C Paria
    Department of Pediatrics, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160 7336, USA
    Science 296:2185-8. 2002
    Implantation involves a series of steps leading to an effective reciprocal signaling between the blastocyst and the uterus...
  85. ncbi Expression of all Wnt genes and their secreted antagonists during mouse blastocyst and postimplantation development
    Caroline Kemp
    Vrije Universiteit Brussel, Lab for Cell Genetics, Brussels, Belgium
    Dev Dyn 233:1064-75. 2005
    ..We also identified expression patterns for the Wnt1, Wnt3a, Wnt6, Wnt7b, Wnt9a, Wnt10b, and Sfrp1 genes at the blastocyst stage...
  86. ncbi REDOX regulation of early embryo development
    Alexandra J Harvey
    Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, Adelaide University, The Queen Elizabeth Hospital, Woodville Road, Woodville SA 5011, Australia
    Reproduction 123:479-86. 2002
    ..Shifts in intracellular REDOX state may also contribute to spatial differences in cell activity, especially after compaction, and perhaps even major embryonic events such as fertilization, genome activation and cellular differentiation...
  87. pmc Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse
    Hehai Wang
    Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, 1125 MRB IV, 2215 B Garland Avenue, Nashville, TN 37232 0656, USA
    Dev Biol 318:112-25. 2008
    It is unknown whether or not tight junction formation plays any role in morula to blastocyst transformation that is associated with development of polarized trophoblast cells and fluid accumulation...
  88. ncbi Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos
    D Corcoran
    School of Agriculture, Food Science and Veterinary Medicine and Centre for Integrative Biology, Conway Institute for Biomolecular and Biomedical Research, College of Life Sciences, University College Dublin, Belfield, Dublin 4, Ireland
    Reproduction 131:651-60. 2006
    ....
  89. ncbi Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells
    Toshihiro Kobayashi
    Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Cell 142:787-99. 2010
    ..To examine the potential for xenogenic approaches in blastocyst complementation, we injected mouse or rat PSCs into rat or mouse blastocysts, respectively, generating ..
  90. pmc X chromosome inactivation is initiated in human preimplantation embryos
    Ilse M van den Berg
    Department of Obstetrics and Gynecology, Subdivision of Reproductive Medicine, Erasmus Medical Center, Rotterdam, The Netherlands
    Am J Hum Genet 84:771-9. 2009
    ..XIST RNA accumulates at the morula and blastocyst stages and is associated with transcriptional silencing of the XIST-coated chromosomal region...
  91. ncbi Comparisons between nulliparous heifers and cows as oocyte donors for embryo production in vitro
    Dimitrios Rizos
    Department of Animal Science, University College Dublin, Lyons Research Farm, Newcastle, County Dublin, Ireland
    Theriogenology 63:939-49. 2005
    ..difference in the percentage of oocytes cleaving after fertilization, or in the percentage reaching the blastocyst stage between heifers and cows...
  92. ncbi Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity
    H Alm
    Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
    Theriogenology 63:2194-205. 2005
    The aim of this present study was to increase the efficiency of blastocyst production from cows after in vitro maturation/fertilization (IVM/IVF) by oocyte selection before maturation...
  93. pmc Modulation of the maternal immune system by the pre-implantation embryo
    Caroline G Walker
    DairyNZ Ltd, Hamilton, New Zealand
    BMC Genomics 11:474. 2010
    ..To gain a more complete picture of these molecular events, genome-wide transcriptional profiles of reproductive day 17 endometrial tissue were determined in pregnant and cyclic Holstein-Friesian dairy cattle...
  94. ncbi Embryo culture medium: which is the best?
    Michelle Lane
    Research Centre for Reproductive Health, Discipline of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, South Australia, Australia
    Best Pract Res Clin Obstet Gynaecol 21:83-100. 2007
    ..It is therefore important to understand the role of each medium component and to identify possible sources of cellular stress to the embryo that will ultimately affect the function and viability of the conceptus...
  95. ncbi The preimplantation embryo: handle with care
    Adam J Watkins
    School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, United Kingdom
    Semin Reprod Med 26:175-85. 2008
    ..We consider how fetal and postnatal phenotype may become susceptible to the plasticity of the preimplantation embryo and the risks for adult health and physiology...
  96. pmc Analysis of imprinted gene expression in normal fertilized and uniparental preimplantation porcine embryos
    Chi Hun Park
    SooAm Biotech Research Foundation, Seoul, Republic of Korea
    PLoS ONE 6:e22216. 2011
    ..several differentially expressed genes (H19, IGF2, XIST and PEG10) were detected amongst the classes at the blastocyst stage of development...
  97. pmc Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building for Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Development 136:3549-56. 2009
    ....
  98. pmc Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development
    Nina Jancar
    Reproductive Unit, Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Slajmerjeva 3, SI 1000 Ljubljana, Slovenia
    J Assist Reprod Genet 24:91-7. 2007
    ..The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate...
  99. ncbi Melatonin increases cleavage rate of porcine preimplantation embryos in vitro
    N Rodriguez-Osorio
    Department of Animal and Dairy Sciences, Mississippi State University, MS 39762, USA
    J Pineal Res 43:283-8. 2007
    ..study determined the effects of melatonin on in vitro porcine embryo development; in particular, cleavage rate, blastocyst rate, and blastocyst cell number...
  100. pmc The polycomb-group gene Ezh2 is required for early mouse development
    D O'Carroll
    Research Institute of Molecular Pathology IMP, A 1030 Vienna, Austria
    Mol Cell Biol 21:4330-6. 2001
    ..Together, these data suggest an essential role for Ezh2 during early mouse development and genetically link Ezh2 with eed and YY1, the only other early-acting Pc-G genes...
  101. ncbi Colony-stimulating factor 2 inhibits induction of apoptosis in the bovine preimplantation embryo
    Barbara Loureiro
    Department of Animal Sciences and D H Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32611 0910, USA
    Am J Reprod Immunol 65:578-88. 2011
    ..Addition of colony-stimulating factor 2 (CSF2) to culture medium increases post-transfer survival of bovine embryos. Here we provide evidence that one mechanism by which CSF2 affects the embryo is through inhibition of apoptosis...

Research Grants65

  1. Predictors of Dietary Quality in Low-Income Pregnant Women
    Eileen R Fowles; Fiscal Year: 2010
    ..undergoes rapid development, are critical because nutrients must be present in proper balance for successful blastocyst implantation...
  2. Human Decidual Leukocytes and Their Placental Ligands
    JACK STROMINGER; Fiscal Year: 2009
    ..remodeling of the uterine spiral arteries to increase blood flow to the developing fetus, implantation of the blastocyst (16 cell stage embryo) into the uterus and maternal-fetal tolerance...
  3. Functional Analysis of the Embryonic Epigenome in a Non-rodent Model
    Mark E Westhusin; Fiscal Year: 2013
    ..HMTs and/or associated genes will be injected into one-cell bovine embryos which wil then be cultured to the blastocyst stage. These will be transferred into recipient females...
  4. Effect of preimplantation embryo culture on adult glucose homeostasis
    Paolo Rinaudo; Fiscal Year: 2013
    ..Txnip mRNA and protein levels are upregulated in blastocyst after in vitro culture;suboptimal culture conditions (Whitten's medium- WM) results in a more intense txnip ..
  5. Paternal Environmental Exposures and Reproductive Outcomes: A Comparison of in Vi
    DEBORAH ANN HANSEN; Fiscal Year: 2013
    ..if aryl hydrocarbon receptor (AHR) activation affects glucose transporter (GLUT) 1 localization in either the blastocyst stage embryo or stromal cell of the uterine environment, thus having a detrimental effect on embryo development ..
  6. Human Decidual Leukocytes and Their Placental Ligands
    Jack L Strominger; Fiscal Year: 2013
    ..remodeling of the uterine spiral arteries to increase blood flow to the developing fetus, implantation of the blastocyst (16 cell stage embryo) into the uterus and maternal- fetal tolerance...
  7. Transgenic Mitomice Models for Treatment of Blinding Diseases
    John Guy; Fiscal Year: 2013
    ..delivered the MTS AAV containing the mutant human ND4 allele to embryonic stem cells by microinjection into the blastocyst of the mouse, generating offspring with progressive retinal ganglion cell (RGC) demise and optic neuropathy with ..
  8. Microinjection Station for Utah Transgenic and Gene Targeting Mouse Core
    SUSAN JENIFER TAMOWSKI; Fiscal Year: 2010
    ..Most gene-targeted mice are generated by microinjecting targeted embryonic stem cells into blastocyst stage embryos...
  9. A CENTER OF MOLECULAR DEVELOPMENTAL HEMATOPOIESIS
    Stuart H Orkin; Fiscal Year: 2013
    ..Cell and Gene Targeting Core that has supported investigators through education and consultation, oocyte and blastocyst microinjections, assistance in embryo and hematological phenotyping, and de novo ES cell derivation...
  10. Derivation of Mature Oocytes from Human Primordial Follicles
    AARON JW HSUEH; Fiscal Year: 2010
    ..Mature oocytes could be retrieved from these ovaries for IVF and blastocyst formation...
  11. Immune Gene Regulation in Human Trophoblast Cells
    Shawn P Murphy; Fiscal Year: 2012
    ..Placental trophoblast cells (TBCs) are the only blastocyst-derived cells in direct contact with maternal blood, and they play essential roles in modulating maternal immune ..
  12. GATA Factor Function in Trophoblast
    Soumen Paul; Fiscal Year: 2013
    ..TE) of developing mouse embryo, regulates expression of key genes in TE lineage, and is involved in morula to blastocyst transformation...
  13. GLUTS AND GLUCOSE TRANSPORT IN THE MOUSE BLASTOCYST
    Kelle H Moley; Fiscal Year: 2012
    ..transport, both diabetic and genetic, which we established during the last grant cycle, all experience increased blastocyst apoptosis and result in significant growth deficiencies, some accompanied by specific congenital malformations...
  14. Hamster: a unique model for studying implantation
    BIBHASH CHANDRA PARIA; Fiscal Year: 2013
    ..biologically and clinically important aspects of implantation: 1) muscular tone of the receptive uterus, 2) blastocyst-uterine cross talk prior to implantation, 3) trophoblast attachment, outgrowth and invasion, and 4) uterine ..
  15. The Function of the Mia3 Gene in Murine Placentation
    Ann E Sutherland; Fiscal Year: 2012
    ..Trophoblast cells arise from the epithelial trophectoderm cells of the blastocyst and progressively differentiate into a variety of subtypes with specialized functions...
  16. AMP-activated protein kinase management will improve oocyte cryopreservation
    Daniel Allen Rappolee; Fiscal Year: 2010
    ..during stress and regulates a transcription factor maintaining potency in 2-cell embryos, blastocysts, and blastocyst-derived placental trophoblast stem cells (TSC)...
  17. COOPERATIVE MULTICENTER REPRODUCTIVE MEDICINE NETWORK
    Christos Coutifaris; Fiscal Year: 2013
    ..It is hypothesized that a single blastocyst transfer results in a delivery rate similar to double blastocyst transfer in good prognosis patients...
  18. Analysis of Hif1alpha-mediated Regulation of Telomerase &Telomeres in Stem Cells
    Richard C Allsopp; Fiscal Year: 2013
    ..whether expression of a mutant Hif11 transgene, which is constitutively stable, in pluripotent stem cells in the blastocyst effects telomerase activity...
  19. Development of normal and dystrophic canine iPS lines
    COLIN EDWARD BISHOP; Fiscal Year: 2010
    ..2) Gene expression profiles of iPS lines will be characterized and compared to a blastocyst derived canine (3) To assess the pluripotency of canine ES and iPS lines in vivo and in vitro.
  20. Adrenomedullin Signaling at the Maternal-Fetal Interface
    Kathleen M Caron; Fiscal Year: 2013
    ..We have shown that maternal AM is involved in the endocrine preparation of the uterus for receptivity to the blastocyst and that a modest 50% reduction in maternal AM levels is sufficient to induce profound reproductive defects ..
  21. Functional Analysis of CDKp110 Protein Kinase
    Jill M Lahti; Fiscal Year: 2010
    ..5, indicating that CDK11p110 and/or CDK11p58 kinase function is essential for post-blastocyst embryonic development...
  22. TEAD4 and Trophoblast Lineage
    Soumen Paul; Fiscal Year: 2013
    ..TEAD4-null embryos do not mature to the blastocyst stage and lack expression of master regulators of trophoblast development, like CDX2, and GATA3...
  23. Oocyte factors for reprogramming to pluripotency
    RENEE A contact REIJO PERA; Fiscal Year: 2010
    ..2) Monitor oocyte development to blastocyst stage following genetic manipulations to suppress or increase expression of key oocyte genes that we identify as ..
  24. Improvement in Oocyte In Vitro Maturation (IVM) Using Microfluidic Culture
    ARTHUR GERSHOWITZ; Fiscal Year: 2010
    ..with media flow will effectively support in vitro oocyte maturation, resulting in maturation, fertilization and blastocyst development percentages greater than those obtained by traditional static culture...
  25. A murine model for placental metabolic reprogramming
    Nicholas P Illsley; Fiscal Year: 2013
    ..technique for lentiviral transduction to deliver HIF-1[unreadable]shRNAmir to the outer cell layer of the blastocyst but not the inner cell mass, leading to placental transduction without effects on the fetus...
  26. Endocannabinoid Signaling During Early Pregnancy
    Sudhansu K Dey; Fiscal Year: 2012
    Synchronized embryonic development to the blastocyst stage, preparation of the receptive uterus and reciprocal embryo-uterine interactions are critical to implantation. Mechanisms that coordinate these events are not clearly understood...
  27. Novel Roles of Pluripotency Regulators in the Early Mouse Embryo
    Wing H Wong; Fiscal Year: 2012
    ..embryo, which for our purpose here, encompasses developmental stages that occur after fertilization and prior to blastocyst formation...
  28. High Throughput Cryopreservation of In Vivo-Derived Swine Embryos
    Randall S Prather; Fiscal Year: 2010
    ..1999), but in some cases they actually develop to the blastocyst stage at a higher rate (Lai et al., 2006;Li et al., 2006)...
  29. Role of Brg1 in Embryo Development and Pluripotency
    JASON GLENN KNOTT; Fiscal Year: 2013
    ..Brg1-deficient embryos arrest around the blastocyst stage, undergo perturbations in gene expression, and exhibit defects in the ICM and trophectoderm...
  30. GLYCOPROTEIN MARKERS OF UTERINE RECEPTIVITY
    Daniel D Carson; Fiscal Year: 2010
    ..In addition, MUC1 is removed at the time of blastocyst attachment in most species to create access to embryo binding sites at the apical surface of uterine epithelia...
  31. MECHANISMS OF MAMMALIAN FERTILIZATION/POLYSPERMY BLOCK
    Richard M Schultz; Fiscal Year: 2012
    ..Ca2+ oscillations or subjecting eggs to hyperstimulation of Ca2+ signaling does not affect development to the blastocyst stage, but differentially affects both gene expression in the blastocysts and implantation/post-implantation ..
  32. Cell Adhesion Mechanisms in Vascular Disease &Thrombosis
    MARK HOWARD GINSBERG; Fiscal Year: 2013
    ..will provide expertise, genomic constructs, genotyping, well characterized murine embryonic stem cells, and blastocyst injections for the purpose of genetic manipulation of mice...
  33. Embryotropic actions of follistatin: mechanisms and translational relevance
    George W Smith; Fiscal Year: 2013
    ..and a functional role for follistatin in promoting enhanced development of in vitro fertilized bovine embryos to blastocyst stage and blastocyst cell allocation to trophectoderm...
  34. Use of OET to assess mouse preimplantation quality for uterine transfer
    MAURICE MARCEL GARCIA; Fiscal Year: 2013
    ..Based (only) on their response to OET assay, he will identify which, among otherwise indistinguishable blastocyst-stage embryos, are most and least likely to result in healthy live birth following transfer (Aim 1)...
  35. Gestational Metabolic Conditions and Autism
    CHERYL KATHERINE WALKER; Fiscal Year: 2013
    ..efficiently address novel hypotheses focused on the gestational environment in which the organism develops from blastocyst to neonate...
  36. Smad4 in blastocyst morphogenesis
    Yijing Chen; Fiscal Year: 2010
    ..from lacking maternally deposited Smad4 and to elucidate the mechanisms by which Smad4 regulates the process of blastocyst morphogenesis...
  37. Directed hematopoietic differentiation of human pluripotent stem cells
    Linzhao Cheng; Fiscal Year: 2013
    ..differentiation from blood-derived and fibroblast-derived human iPS cells, in comparison with that of blastocyst-derived hES cells...
  38. Molecular signaling in uterine receptivity to implantation
    Sudhansu K Dey; Fiscal Year: 2013
    ..for implantation in placental mammals is an effective two-way interaction between an implantation- competent blastocyst and the receptive uterus. The blastocyst will implant only when this molecular dialogue is established...
  39. Epigenetics of Dental Stem Cells: Markers and CP27 Function
    Xianghong Luan; Fiscal Year: 2010
    ..In preliminary studies, we found that embryos lacking the CP27 gene developed normally until blastocyst stage, but failed to survive shortly after implantation...
  40. ASPECTS OF BLASTOCYST IMPLANTATION
    Sudhansu K Dey; Fiscal Year: 2010
    ..prerequisite for mammalian reproduction is an effective reciprocal interaction between an implantation-competent blastocyst and the receptive uterus. The blastocyst will implant only when this molecular dialogue is established...
  41. PDGF-B signaling in fetal hematopoietic stem cell microenvironments
    HANNA KATRI ANNIKKI MIKKOLA; Fiscal Year: 2012
    ..a novel technology for placental trophoblast specific gene targeting by using lentiviral infection at the blastocyst stage...
  42. UCD MUTANT MOUSE REGIONAL RESOURCE CENTER
    KC KENT LLOYD; Fiscal Year: 2013
    ..g., gene trap ES cells) and initiating new services (e.g., blastocyst injection, ICSI) that have added scientific value to mouse models and enhanced utilization of the MMRRC by ..
  43. UCD MUTANT MOUSE REGIONAL RESOURCE CENTER
    KC KENT LLOYD; Fiscal Year: 2011
    ..g., gene trap ES cells) and initiating new services (e.g., blastocyst injection, ICSI) that have added scientific value to mouse models and enhanced utilization of the MMRRC by ..
  44. Comparative phenotypic, functional, and molecular analysis of ESC and iPSC
    Peter J Park; Fiscal Year: 2010
    ..If proven equivalent-both functionally and molecularly-to blastocyst-derived human embryonic stem cells, iPS cells will facilitate research, quell contentious public debate, and ..
  45. Regulation of embryonic epithelial morphogenesis
    Shaohua Li; Fiscal Year: 2011
    ..During mouse peri- implantation development, the undifferentiated inner cell mass of the blastocyst is converted from a nonpolar cell aggregate to a highly organized epithelial cyst...
  46. Reproduction of Transgenic Pigs by Nuclear Transplantation
    Randall S Prather; Fiscal Year: 2010
    ..aim will be to correlate the transcriptional profile with the pattern of DNA methylation in normal and cloned blastocyst stage embryos. The second aim is to perform a similar experiment at later stages of development, i.e...
  47. Co-axial Microinjection System for Freezing and Biopsy of Early Embryos
    PAUL JON TAYLOR; Fiscal Year: 2013
    ..The real significance of this device is that it allows control and manipulation of embryos at the hatched blastocyst stage of development, after hatching from the zona pellucida and before implantation in the uterus...
  48. Biological Insights into Dynamics of Stem Cell Differentiation and Misbehavior
    Joseph Wu; Fiscal Year: 2009
    Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos...
  49. OXIDATIVE DAMAGE AND IRON REQUIREMENTS OF THE BLASTOCYST
    COLE MANES; Fiscal Year: 1991
    The long-term objective is to clarify the nutritional requirements of the blastocyst period in mammalian development, so that the successful maintenance of normal blastocysts may be accomplished in vitro...
  50. PAF AND PRE-IMPLANTATION/IMPLANTATION PHENOMENA
    Michael Harper; Fiscal Year: 1991
    ..on the blastomeres in early stage embryos, and on the cells of the trophoblast and the inner cell mass at the blastocyst stage, 4) correlate changes in vascular permeability of the rabbit uterus with administration of synthetic PAF ..
  51. ANALYSIS OF CELL LINEAGE IN EMBRYOS
    Roger Pedersen; Fiscal Year: 1992
    ..the cell lineages of a model marsupial, the gray short-tailed opossum (Monodelphis domestica) during cleavage, blastocyst formation, and gastrulation...
  52. ACTIONS OF ESTROGEN IN MATURING GRAAFIAN FOLLICLES
    Johannes Veldhuis; Fiscal Year: 2005
    ..Estrogen and progesterone act coordinately to mature the endometrium for implantation of the blastocyst and time the preovulatory gonadotropin surge...
  53. IGF INDUCED TOXICITY IN PREIMPLANTATION EMBRYOS
    Kelle Moley; Fiscal Year: 2003
    ..levels, however, serum levels of bioactive IGF-1 are elevated and it is known that high IGF-1 levels impair blastocyst development...
  54. INTERFERON EFFECTS ON INTERMEDIATE FILAMENTS IN TUMOR CE
    DIANNE BUCKMAN; Fiscal Year: 1992
    ..They are initially located within the trophoblast layer of the blastocyst embryo and ultimately found in a limited number of normal adult differentiated tissues...
  55. HISTAMINE LIGAND RECEPTOR SIGNALING IN IMPLANTATION
    BIBHASH PARIA; Fiscal Year: 2002
    DESCRIPTION (adapted from the applicant's abstract): Blastocyst implantation involves complex synchronized interactions between the heterogeneous cell types of the uterus and embryo...
  56. ENDOMETRIAL RESPONSE TO CONCEPTUS SECRETORY PROTEINS
    Fuller Bazer; Fiscal Year: 1990
    We shall study some of the biochemical interactions which occur between the preimplantation blastocyst and the endometrium which allow for the establishment of pregnancy. We plan to purify two early products of the sheep conceptus...
  57. RAPID PRENATAL SEXING OF CLEAVING MOUSE EMBRYOS
    JON GORDON; Fiscal Year: 1992
    ..cell conceptus and determine the sex of the biopsied blastomere as the remainder of the embryo cleaves to the blastocyst stage...
  58. IGF INDUCED TOXICITY IN PREIMPLANTATION EMBRYOS
    Kelle Moley; Fiscal Year: 2007
    ..All three conditions have insulin resistance in common. The mammalian blastocyst is an insulin/IGF-1 sensitive tissue...
  59. INTERACTION BETWEEN BLASTOCYST AND UTERINE ENDOMETRIUM
    R Roberts; Fiscal Year: 1999
    ..It is anticipated that the proteins comprise additional bioactive components involved in conceptus-maternal signalling during early pregnancy. ..
  60. MARKERS OF ENDOMETRIAL RECEPTIVITY DURING IMPLANTATION
    INDRANI BAGCHI; Fiscal Year: 2000
    ..The endometrium can accept a developing blastocyst for implantation only during a limited period of time, termed the receptive phase, during the menstrual cycle...
  61. ROLE OF ENVIRONMENTAL MUTAGENS IN POOR PREGNANCY OUTCOME
    Philip Iannaccone; Fiscal Year: 1990
    ..Define chemical exposures in vitro at discrete development periods will be used. Chemically-treated blastocyst stage embryos will be transferred to pseudopregnant surrogate mothers and subsequent post-implantation ..
  62. Reprogramming of X-linked genes by nuclear transfer
    X Tian; Fiscal Year: 2003
    ..In mice and cattle, X chromosome inactivation (XCI) is established at blastocyst stage with the paternal X chromosome (XP) preferentially inactivated (XiP) in the trophectoderm...
  63. T CELL IMMUNOREGULATION BY TRYPTOPHAN DEPLETION
    Andrew Mellor; Fiscal Year: 2002
    ..by an interferon-gamma-inducible gene expressed by macrophages that suppress T embryo loss a few days after blastocyst implantation in mice carrying allogeneic conceptus...
  64. CELL DEATH, CHALONES, AND CYTOTOXIC METABOLITES
    Martin Murphy; Fiscal Year: 1993
    During experiments on the mechanisms of regulation of embryonal carcinoma cells by the blastocyst, a soluble factor in blastocele fluid was identified that causes programmed cell death of some embryonal carcinoma and inner cell mass cells ..
  65. BIOCHEMICAL AND GENETIC ANALYSES OF AN EMBRYONIC ANTIGEN
    Paulette McCormick; Fiscal Year: 1991
    ..At the blastocyst stage, however, the molecule migrates as a broad, heterogeneous band ranging from 90 to 110 kDa...