myoblasts

Summary

Summary: Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Top Publications

  1. pmc The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation
    Jian Fu Chen
    Carolina Cardiovascular Biology Center, Department of Cell and Developmental Biology, University of North Carolina Chapel Hill, North Carolina 27599, USA
    Nat Genet 38:228-33. 2006
  2. pmc microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7
    Jian Fu Chen
    McAllister Heart Institute, Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 190:867-79. 2010
  3. ncbi MicroRNA-26a targets the histone methyltransferase Enhancer of Zeste homolog 2 during myogenesis
    Chung Fai Wong
    CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia 4067, QLD, Australia
    J Biol Chem 283:9836-43. 2008
  4. pmc Myogenic factors that regulate expression of muscle-specific microRNAs
    Prakash K Rao
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 103:8721-6. 2006
  5. ncbi Notch-mediated restoration of regenerative potential to aged muscle
    Irina M Conboy
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305 5235, USA
    Science 302:1575-7. 2003
  6. ncbi Interleukin-6 is an essential regulator of satellite cell-mediated skeletal muscle hypertrophy
    Antonio L Serrano
    Program on Differentiation and Cancer, Center for Genomic Regulation CRG and Center for Neurodegenerative Diseases CIBERNED, Pompeu Fabra University, Barcelona, Spain
    Cell Metab 7:33-44. 2008
  7. pmc DUX4 activates germline genes, retroelements, and immune mediators: implications for facioscapulohumeral dystrophy
    Linda N Geng
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Dev Cell 22:38-51. 2012
  8. pmc Notch3 and Mef2c proteins are mutually antagonistic via Mkp1 protein and miR-1/206 microRNAs in differentiating myoblasts
    Jeffrey Gagan
    Department of Biochemistry and Molecular Genetics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Biol Chem 287:40360-70. 2012
  9. ncbi A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis
    Andrew S Brack
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, 94305 5235 USA
    Cell Stem Cell 2:50-9. 2008
  10. pmc Expression of a mutant lamin A that causes Emery-Dreifuss muscular dystrophy inhibits in vitro differentiation of C2C12 myoblasts
    Catherine Favreau
    Departement de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universités Paris 6 and 7, 75251 Paris Cedex 05, France
    Mol Cell Biol 24:1481-92. 2004

Detail Information

Publications265 found, 100 shown here

  1. pmc The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation
    Jian Fu Chen
    Carolina Cardiovascular Biology Center, Department of Cell and Developmental Biology, University of North Carolina Chapel Hill, North Carolina 27599, USA
    Nat Genet 38:228-33. 2006
    ..and miR-133 have distinct roles in modulating skeletal muscle proliferation and differentiation in cultured myoblasts in vitro and in Xenopus laevis embryos in vivo...
  2. pmc microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7
    Jian Fu Chen
    McAllister Heart Institute, Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 190:867-79. 2010
    ..Therefore, our experiments suggest that microRNAs participate in a regulatory circuit that allows rapid gene program transitions from proliferation to differentiation...
  3. ncbi MicroRNA-26a targets the histone methyltransferase Enhancer of Zeste homolog 2 during myogenesis
    Chung Fai Wong
    CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia 4067, QLD, Australia
    J Biol Chem 283:9836-43. 2008
    ..In this study, we profiled the expression of miRNA during myogenesis, from proliferating myoblasts through to terminally differentiated myotubes...
  4. pmc Myogenic factors that regulate expression of muscle-specific microRNAs
    Prakash K Rao
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 103:8721-6. 2006
    ..miR-1, miR-133 and miR-206, are robustly induced during the myoblast-myotube transition, both in primary human myoblasts and in the mouse mesenchymal C2C12 stem cell line...
  5. ncbi Notch-mediated restoration of regenerative potential to aged muscle
    Irina M Conboy
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305 5235, USA
    Science 302:1575-7. 2003
    ..age, resident precursor cells (satellite cells) had a markedly impaired propensity to proliferate and to produce myoblasts necessary for muscle regeneration...
  6. ncbi Interleukin-6 is an essential regulator of satellite cell-mediated skeletal muscle hypertrophy
    Antonio L Serrano
    Program on Differentiation and Cancer, Center for Genomic Regulation CRG and Center for Neurodegenerative Diseases CIBERNED, Pompeu Fabra University, Barcelona, Spain
    Cell Metab 7:33-44. 2008
    ..These findings unveil a role for IL-6 in hypertrophic muscle growth and provide mechanistic evidence for the contribution of satellite cells to this process...
  7. pmc DUX4 activates germline genes, retroelements, and immune mediators: implications for facioscapulohumeral dystrophy
    Linda N Geng
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Dev Cell 22:38-51. 2012
    ..These findings suggest specific mechanisms of FSHD pathology and identify candidate biomarkers for disease diagnosis and progression...
  8. pmc Notch3 and Mef2c proteins are mutually antagonistic via Mkp1 protein and miR-1/206 microRNAs in differentiating myoblasts
    Jeffrey Gagan
    Department of Biochemistry and Molecular Genetics, Robert M Berne Cardiovascular Research Center, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Biol Chem 287:40360-70. 2012
    ..Notch3 is expressed in myogenic precursors, but its function is not well known...
  9. ncbi A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis
    Andrew S Brack
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, 94305 5235 USA
    Cell Stem Cell 2:50-9. 2008
    ....
  10. pmc Expression of a mutant lamin A that causes Emery-Dreifuss muscular dystrophy inhibits in vitro differentiation of C2C12 myoblasts
    Catherine Favreau
    Departement de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universités Paris 6 and 7, 75251 Paris Cedex 05, France
    Mol Cell Biol 24:1481-92. 2004
    ..Our hypothesis is that lamin A mutated at arginine 453 fails to build a functional scaffold and/or to maintain the chromatin compartmentation required for differentiation of myoblasts into myocytes.
  11. pmc Myostatin negatively regulates satellite cell activation and self-renewal
    Seumas McCroskery
    Animal Genomics, AgResearch, Hamilton 2015, New Zealand
    J Cell Biol 162:1135-47. 2003
    ..be due to increased self-renewal and delayed expression of the differentiation gene (myogenin) in Mstn-/- adult myoblasts. Taken together, these results suggest that Myostatin is a potent negative regulator of satellite cell ..
  12. pmc A unique library of myogenic cells from facioscapulohumeral muscular dystrophy subjects and unaffected relatives: family, disease and cell function
    Sachiko Homma
    Neuromuscular Biology and Disease Group, Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
    Eur J Hum Genet 20:404-10. 2012
    ....
  13. pmc Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes
    Petr Dmitriev
    UMR 8126, Univ Paris Sud 11, CNRS, Institut de Cancerologie Gustave Roussy, 39, rue Camille Desmoulins, Villejuif 94805, France
    BMC Genomics 14:265. 2013
    ..miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis...
  14. pmc Hypoxia-inducible factor 1-α induces miR-210 in normoxic differentiating myoblasts
    Lucia Cicchillitti
    IRCCS Policlinico San Donato, 20097 Milan, Italy
    J Biol Chem 287:44761-71. 2012
    ..We found that miR-210 was induced in normoxic myoblasts upon myogenic differentiation both in vitro and in vivo...
  15. pmc Microrna-221 and microrna-222 modulate differentiation and maturation of skeletal muscle cells
    Beatrice Cardinali
    Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
    PLoS ONE 4:e7607. 2009
    ..miRNAs have been shown to play crucial roles in muscle development and in regulation of muscle cell proliferation and differentiation...
  16. pmc Gene expression during normal and FSHD myogenesis
    Koji Tsumagari
    Human Genetics Program, Tulane Medical School, New Orleans, LA, USA
    BMC Med Genomics 4:67. 2011
    ..Within each repeat unit is a gene, DUX4, that can encode a protein containing two homeodomains. A DUX4 transcript derived from the last repeat unit in a contracted array is associated with pathogenesis but it is unclear how...
  17. pmc RNA transcripts, miRNA-sized fragments and proteins produced from D4Z4 units: new candidates for the pathophysiology of facioscapulohumeral dystrophy
    Lauren Snider
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Hum Mol Genet 18:2414-30. 2009
    ..Together, we have identified new sense and anti-sense RNA transcripts, novel mRNAs and mi/siRNA-sized RNA fragments generated from the D4Z4 units that are new candidates for the pathophysiology of FSHD...
  18. ncbi IL-4 acts as a myoblast recruitment factor during mammalian muscle growth
    Valerie Horsley
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, GA 30322, USA
    Cell 113:483-94. 2003
    Skeletal muscle formation and growth require the fusion of myoblasts to form multinucleated myofibers or myotubes, but few molecules are known to regulate myoblast fusion in mammals...
  19. ncbi Inhibition of miR-214 expression represses proliferation and differentiation of C2C12 myoblasts
    Yang Feng
    Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China
    Cell Biochem Funct 29:378-83. 2011
    ..The results showed that miR-214 was expressed both in myoblasts and in myotubes and was upregulated during differentiation...
  20. ncbi C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization
    S Burattini
    Istituto di Scienze Morfologiche, Facoltà di Scienze Motorie University of Urbino Carlo Bo, Urbino, Italy
    Eur J Histochem 48:223-33. 2004
    In this study, the differentiation of C2C12 cells, a primary line of murine myoblasts, was investigated by a multiple technical approach...
  21. ncbi Cellular senescence in human myoblasts is overcome by human telomerase reverse transcriptase and cyclin-dependent kinase 4: consequences in aging muscle and therapeutic strategies for muscular dystrophies
    Chun Hong Zhu
    Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9039, USA
    Aging Cell 6:515-23. 2007
    Cultured human myoblasts fail to immortalize following the introduction of telomerase...
  22. pmc Myoblasts from affected and non-affected FSHD muscles exhibit morphological differentiation defects
    Marietta Barro
    INSERM, ERI25, Montpellier, France
    J Cell Mol Med 14:275-89. 2010
    ..Several studies have investigated possible pathophysiological pathways in FSHD myoblasts and mature muscle cells but some of these reports were apparently in contradiction...
  23. pmc Myoblast fusion: lessons from flies and mice
    Susan M Abmayr
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Development 139:641-56. 2012
    The fusion of myoblasts into multinucleate syncytia plays a fundamental role in muscle function, as it supports the formation of extended sarcomeric arrays, or myofibrils, within a large volume of cytoplasm...
  24. ncbi A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis
    Roddy S O'Connor
    Graduate Program in Molecular and Systems Pharmacology, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 120:149-59. 2007
    ..inhibition of NFAT5 transcriptional activity caused both a migratory and differentiation defect in cultured myoblasts. We identified Cyr61 as a target of NFAT5 signaling in skeletal muscle cells...
  25. pmc Assembly of centrosomal proteins and microtubule organization depends on PCM-1
    Alexander Dammermann
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 159:255-66. 2002
    ....
  26. pmc Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation
    Daniela Deponti
    Department of Histology and Medical Embryology, University of Roma La Sapienza, 00161 Rome, Italy
    J Cell Biol 179:305-19. 2007
    ..Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration...
  27. pmc UTX mediates demethylation of H3K27me3 at muscle-specific genes during myogenesis
    Shayesta Seenundun
    Regenerative Medicine Program, Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada
    EMBO J 29:1401-11. 2010
    ..Thus, removal of repressive H3K27me3 marks by UTX occurs through targeted recruitment followed by spreading across the gene...
  28. pmc Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells
    Jerome E Lee
    Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado, USA
    PLoS ONE 5:e11201. 2010
    ..Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific...
  29. pmc JAK1-STAT1-STAT3, a key pathway promoting proliferation and preventing premature differentiation of myoblasts
    Luguo Sun
    Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong, China
    J Cell Biol 179:129-38. 2007
    Skeletal muscle stem cell-derived myoblasts are mainly responsible for postnatal muscle growth and injury-induced muscle regeneration...
  30. pmc MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3
    Hiroyuki Hirai
    Stem Cell Institute, Department of Neurology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Cell Biol 191:347-65. 2010
    ..We noticed that MyoD(-/-) myoblasts display remarkable resistance to apoptosis by down-regulation of miR-1 (microRNA-1) and miR-206 and by up-..
  31. pmc An invasive podosome-like structure promotes fusion pore formation during myoblast fusion
    Kristin L Sens
    Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Cell Biol 191:1013-27. 2010
    ..Our studies uncover a novel invasive podosome-like structure (PLS) in a developing tissue and reveal a previously unrecognized function of PLSs in facilitating cell membrane juxtaposition and fusion...
  32. ncbi Derivation of engraftable skeletal myoblasts from human embryonic stem cells
    Tiziano Barberi
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, New York, New York 10021, USA
    Nat Med 13:642-8. 2007
    ..cell sorting (FACS)-mediated purification yielded multipotent mesenchymal precursors and skeletal myoblasts. Skeletal muscle cells undergo in vitro maturation resulting in myotube formation and spontaneous twitching...
  33. pmc miR-29 targets Akt3 to reduce proliferation and facilitate differentiation of myoblasts in skeletal muscle development
    W Wei
    Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, PRC
    Cell Death Dis 4:e668. 2013
    ....
  34. ncbi p38-dependent phosphorylation of the mRNA decay-promoting factor KSRP controls the stability of select myogenic transcripts
    Paola Briata
    Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy
    Mol Cell 20:891-903. 2005
    ..The extracellular signal-activated p38 MAP kinase (p38) pathway plays a fundamental role in conversion of myoblasts to differentiated myocytes...
  35. ncbi Transcriptional profiling and regulation of the extracellular matrix during muscle regeneration
    Sean C Goetsch
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8573, USA
    Physiol Genomics 14:261-71. 2003
    ..We conclude that muscle regeneration is a complex process that requires the coordinated modulation of the inflammatory response, myogenic progenitor cells, growth factors, and ECM for complete restoration of muscle architecture...
  36. pmc Loss of miR-29 in myoblasts contributes to dystrophic muscle pathogenesis
    Lijun Wang
    Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China
    Mol Ther 20:1222-33. 2012
    ..Mechanistic studies revealed that loss of miR-29 in muscle precursor cells (myoblasts) promotes their transdifferentiation into myofibroblasts through targeting extracellular molecules including ..
  37. pmc Smad7 promotes and enhances skeletal muscle differentiation
    Helen D Kollias
    Department of Biology, 327 Farquharson, LSB, York University, 4700 Keele St, Toronto M3J 1P3 Ontario, Canada
    Mol Cell Biol 26:6248-60. 2006
    ..Taken together, these data implicate Smad7 as a fundamental regulator of differentiation in skeletal muscle cells...
  38. pmc ADP-ribosylation factor 6 regulates mammalian myoblast fusion through phospholipase D1 and phosphatidylinositol 4,5-bisphosphate signaling pathways
    Anne Sophie Bach
    Universités Montpellier 2 et 1, Centre de Recherche en Biochimie Macromoleculaire, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5237, Institut Fédératif de Recherche 122 1919 Route de Mende, 34293 Montpellier, France
    Mol Biol Cell 21:2412-24. 2010
    ..ARF6 activity is increased at the time of myoblast fusion and is required for its implementation in mouse C2C12 myoblasts. Specifically, at the onset of myoblast fusion, ARF6 is associated with the multiproteic complex that contains M-..
  39. ncbi A new pro-migratory activity on human myogenic precursor cells for a synthetic peptide within the E domain of the mechano growth factor
    Philippe Mills
    Unité de Recherche en Génétique Humaine, CHUQ CHUL, 2705 Boul Laurier, Ste Foy, QC, Canada G1V 4G2
    Exp Cell Res 313:527-37. 2007
    ..Those results reinforce the possibility that the IGF-1Ec isoform may produce an E domain peptide that can act as a cytokine...
  40. pmc Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin
    Chuanxi Cai
    Departments of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Biol Chem 284:15894-902. 2009
    ....
  41. pmc Transforming growth factor-beta1 induces transdifferentiation of myoblasts into myofibroblasts via up-regulation of sphingosine kinase-1/S1P3 axis
    Francesca Cencetti
    Dipartimento di Scienze Biochimiche, Universita di Firenze, 50134, Firenze, Italy
    Mol Biol Cell 21:1111-24. 2010
    ..However, the molecular mechanisms implicated in TGFbeta1-dependent transdifferentiation of myoblasts into myofibroblasts are presently unknown...
  42. pmc A Suv39h-dependent mechanism for silencing S-phase genes in differentiating but not in cycling cells
    Slimane Ait-Si-Ali
    UPR 9079 CNRS Labellisée Ligue Nationale Contre le Cancer, Institut Andre Lwoff, Villejuif, France
    EMBO J 23:605-15. 2004
    ..Finally, Suv39h-depleted myoblasts were unable to express early or late muscle differentiation markers...
  43. ncbi Biological progression from adult bone marrow to mononucleate muscle stem cell to multinucleate muscle fiber in response to injury
    Mark A Labarge
    Baxter Laboratory for Genetic Pharmacology, Department of Microbiology and Immunology, Department of Molecular Pharmacology, Stanford University School of Medicine, CCSR 4215, Stanford, CA 94305, USA
    Cell 111:589-601. 2002
    ..The stress-induced progression of BMDC to muscle satellite cell to muscle fiber results in a contribution to as many as 3.5% of muscle fibers and is due to developmental plasticity in response to environmental cues...
  44. pmc Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis
    Yuting Sun
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA
    J Cell Biol 189:1157-69. 2010
    ..that expression of the muscle-specific micro-RNA (miRNA) miR-1 is regulated by mTOR both in differentiating myoblasts and in mouse regenerating skeletal muscle...
  45. pmc Myoblast fusion: when it takes more to make one
    Kate Rochlin
    Program in Developmental Biology, Sloan Kettering Institute, New York, NY 10065, USA
    Dev Biol 341:66-83. 2010
    ..Taken together, comparisons across three model systems are illuminating a once elusive process, providing exciting new insights and a useful framework of genes and mechanisms...
  46. pmc MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation
    Soji Sebastian
    Centre for Cellular and Molecular Biology, Hyderabad, 500 007 India
    Proc Natl Acad Sci U S A 106:4719-24. 2009
    ..MLL5 is induced in quiescent myoblasts and regulates both the cell cycle and differentiation via a hierarchy of chromatin and transcriptional ..
  47. pmc Microenvironmental VEGF concentration, not total dose, determines a threshold between normal and aberrant angiogenesis
    Clare R Ozawa
    Baxter Laboratory in Genetic Pharmacology, Stanford University School of Medicine, Stanford, California 94305 5175, USA
    J Clin Invest 113:516-27. 2004
    ..VEGF dosage and the morphology and function of newly formed blood vessels by implanting retrovirally transduced myoblasts that constitutively express VEGF164 into muscles of adult mice...
  48. ncbi C2C12 myoblast sensitivity to different apoptotic chemical triggers
    Sara Salucci
    DISUAN, Universita degli Studi di Urbino Carlo Bo, Urbino, Italy
    Micron 41:966-73. 2010
    ..To induce apoptosis, C2C12 myoblasts have been exposed to H(2)O(2), staurosporine, cisplatin and etoposide, at different doses and incubation times, ..
  49. ncbi MSX1 cooperates with histone H1b for inhibition of transcription and myogenesis
    Hansol Lee
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Science 304:1675-8. 2004
    ..Moreover, Msx1 and H1b cooperate to inhibit muscle differentiation in cell culture and in Xenopus animal caps. Our findings define a previously unknown function for "linker" histones in gene-specific transcriptional regulation...
  50. pmc TNF-alpha regulates myogenesis and muscle regeneration by activating p38 MAPK
    Shuen Ei Chen
    Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Physiol Cell Physiol 292:C1660-71. 2007
    ..role of TNF-alpha in myogenic activation of p38, we tried to determine whether p38 activation in differentiating myoblasts requires autocrine TNF-alpha, and whether forced activation of p38 rescues impaired myogenesis and regeneration ..
  51. ncbi Sca-1 negatively regulates proliferation and differentiation of muscle cells
    Patrick O Mitchell
    Department of Pharmacology, Emory University School of Medicine, O W Rollins Research Center, Atlanta, GA 30322, USA
    Dev Biol 283:240-52. 2005
    ..Upon exposure to appropriate stimuli, satellite cells produce progeny myoblasts. Heterogeneity within a population of myoblasts ensures that a subset of myoblasts readily differentiate to form ..
  52. ncbi Replicative aging down-regulates the myogenic regulatory factors in human myoblasts
    Anne Bigot
    INSERM, Université Pierre and Marie Curie Paris 6, UMR S 787, Institut de Myologie, Paris 75013, France
    Biol Cell 100:189-99. 2008
    ..The myogenic differentiation of human myoblasts is regulated by the muscle-specific regulatory factors...
  53. pmc Mutations of the FHL1 gene cause Emery-Dreifuss muscular dystrophy
    Lucie Gueneau
    INSERM, U974, Paris, France
    Am J Hum Genet 85:338-53. 2009
    ..patients and that this was associated with a severe delay in myotube formation in the two patients for whom myoblasts were available...
  54. pmc Ectopic expression of cyclin D3 corrects differentiation of DM1 myoblasts through activation of RNA CUG-binding protein, CUGBP1
    Elizabeth Salisbury
    Department of Pathology, Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA
    Exp Cell Res 314:2266-78. 2008
    ..In normal myoblasts, Akt kinase phosphorylates CUGBP1 at Ser28 and increases interactions of CUGBP1 with cyclin D1 mRNA...
  55. ncbi Morphological alterations induced by doxorubicin on H9c2 myoblasts: nuclear, mitochondrial, and cytoskeletal targets
    Vilma A Sardao
    Center for Neurosciences and Cellular Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal
    Cell Biol Toxicol 25:227-43. 2009
    ..The purpose of the present work was to evaluate Dox-induced morphological alterations in H9c2 myoblasts, focusing especially on the nuclei, mitochondria, and structural fibrous proteins...
  56. ncbi Diversification of muscle types: recent insights from Drosophila
    Vanessa Tixier
    GReD, INSERM U931, CNRS UMR6247, Clermont University, Faculty of Medicine, 28 Place Henri Dunant, Clermont Ferrand, France
    Exp Cell Res 316:3019-27. 2010
    ..Here we present an overview of Drosophila muscle development with a special focus on the diversification of muscle types and the genes that control acquisition of distinct muscle properties...
  57. ncbi TGF-beta's delay skeletal muscle progenitor cell differentiation in an isoform-independent manner
    Elske J Schabort
    Department of Physiological Sciences, University of Stellenbosch, Private Bag X1, Stellenbosch 7602, South Africa
    Exp Cell Res 315:373-84. 2009
    ..These results demonstrate that TGF-beta promotes proliferation and delays differentiation of C2C12 myoblasts in an isoform-independent manner.
  58. pmc Modulation of cell cycle progression by 5-azacytidine is associated with early myogenesis induction in murine myoblasts
    Anna Montesano
    Department of Biomedical Sciences for Health, University of Milan, Milan, Italy
    Int J Biol Sci 9:391-402. 2013
    Myogenesis is a multistep process, in which myoblasts withdraw from the cell cycle, cease to divide, elongate and fuse to form multinucleated myotubes...
  59. pmc NF-kappaB regulation of YY1 inhibits skeletal myogenesis through transcriptional silencing of myofibrillar genes
    Huating Wang
    Human Cancer Genetics Program, The Ohio State University College of Medicine, Columbus, OH 43210, USA
    Mol Cell Biol 27:4374-87. 2007
    ..To gain insight into these mechanisms, gene expression profiling was examined in C2C12 myoblasts devoid of NF-kappaB activity...
  60. pmc TACE release of TNF-alpha mediates mechanotransduction-induced activation of p38 MAPK and myogenesis
    Mei Zhan
    Department of Medicine, Baylor College of Medicine, One Baylor Plaza 520B, Houston, TX 77030, USA
    J Cell Sci 120:692-701. 2007
    ..Here we show that mechanical stimulation of myoblasts activates p38 and myogenesis through stimulating TNF-alpha release by TNF-alpha converting enzyme (TACE)...
  61. pmc Attenuation of p38-mediated miR-1/133 expression facilitates myoblast proliferation during the early stage of muscle regeneration
    Duo Zhang
    Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China
    PLoS ONE 7:e41478. 2012
    ....
  62. pmc Generation of isogenic D4Z4 contracted and noncontracted immortal muscle cell clones from a mosaic patient: a cellular model for FSHD
    Yvonne D Krom
    Leiden University Medical Center, Leiden, The Netherlands
    Am J Pathol 181:1387-401. 2012
    ..These cell lines will facilitate pairwise comparisons to identify FSHD-specific differences and are expected to create new opportunities for high-throughput drug screens...
  63. ncbi MiR-20a and miR-106b negatively regulate autophagy induced by leucine deprivation via suppression of ULK1 expression in C2C12 myoblasts
    Hao Wu
    Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
    Cell Signal 24:2179-86. 2012
    ..may participate in regulating leucine deprivation induced autophagy via suppression of ULK1 expression in C2C12 myoblasts. We showed that leucine deprivation downregulated miR-20a and miR-106b expression via suppression of their ..
  64. pmc H19 antisense RNA can up-regulate Igf2 transcription by activation of a novel promoter in mouse myoblasts
    Van Giang Tran
    Institut de Genetique Moleculaire de Montpellier, UMR 5535 CNRS Université Montpellier II, Montpellier, France
    PLoS ONE 7:e37923. 2012
    ..Our work contributes, in conjunction with other recent findings, to open new horizons to our understanding of Igf2 gene regulation and functions of the 91H/H19 RNAs in normal and pathological conditions...
  65. pmc PED/PEA-15 induces autophagy and mediates TGF-beta1 effect on muscle cell differentiation
    S Iovino
    Department of Cellular and Molecular Biology and Pathology, University of Naples Federico II, Italy
    Cell Death Differ 19:1127-38. 2012
    ..PED/PEA-15 underwent rapid induction upon TGF-beta1 exposure of L6 and C2C12 myoblasts, accompanied by impaired differentiation into mature myotubes...
  66. ncbi Type I insulin-like growth factor receptor signaling in skeletal muscle regeneration and hypertrophy
    A Philippou
    Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Goudi Athens, Greece
    J Musculoskelet Neuronal Interact 7:208-18. 2007
    ..Differentiation of satellite cells into myoblasts involves the regulation of skeletal muscle-specific proteins belonging to the family of myogenic regulatory ..
  67. pmc Identification of valid reference genes during the differentiation of human myoblasts
    Jens Stern-Straeter
    Department of Otolaryngology, Head and Neck Surgery, University Hospital Mannheim, University of Heidelberg, 68167 Mannheim, Germany
    BMC Mol Biol 10:66. 2009
    ..The geNorm, NormFinder and BestKeeper software programs were used to ascertain the most suitable RG to normalize the RNA input...
  68. pmc Intramuscular transplantation of human postnatal myoblasts generates functional donor-derived satellite cells
    Daniel Skuk
    Unité de Recherche en Génétique Humaine, Centre hospitalier de l Universite Laval, Quebec City, Quebec, Canada
    Mol Ther 18:1689-97. 2010
    ..property was fully reported following myoblast transplantation in mice but remains poorly studied with human myoblasts. In this study, we provide evidence that the intramuscular transplantation of postnatal human myoblasts in ..
  69. pmc BMP antagonists enhance myogenic differentiation and ameliorate the dystrophic phenotype in a DMD mouse model
    Songting Shi
    Department of Molecular Cell Biology and Centre for Biomedical Genetics, Leiden, The Netherlands
    Neurobiol Dis 41:353-60. 2011
    ..differentiation and in a previous study we found an increased expression of a BMP family member BMP4 in DMD myoblasts. The aim of the current study was therefore to investigate whether inhibition of BMP signaling could be ..
  70. ncbi Innate inflammatory cells are not responsible for early death of donor myoblasts after myoblast transfer therapy
    Leanne M Sammels
    Discipline of Microbiology M502, School of Biomedical and Chemical Sciences, University of Western Australia, QEII Medical Center, Nedlands, Perth, W A 6009, Australia
    Transplantation 77:1790-7. 2004
    ..The rapid disappearance of donor myoblasts from transplanted muscles after MTT is one of the most controversial and significant obstacles facing research ..
  71. pmc Reduction of the rate of protein translation in patients with myotonic dystrophy 2
    Claudia Huichalaf
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Neurosci 29:9042-9. 2009
    ..of proteins of translational apparatus in DM2 correlates with a reduction of a rate of protein synthesis in myoblasts from DM2 patients...
  72. ncbi Insulin-mimetic action of conglutin-γ, a lupin seed protein, in mouse myoblasts
    I Terruzzi
    Nutrition Metabolism Unit, San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy
    Nutr Metab Cardiovasc Dis 21:197-205. 2011
    ....
  73. ncbi STIM1- and Orai1-dependent store-operated calcium entry regulates human myoblast differentiation
    Basile Darbellay
    Department of Clinical Neurosciences, Faculty of Medicine, University of Geneva, Switzerland
    J Biol Chem 284:5370-80. 2009
    Our previous work on human myoblasts suggested that a hyperpolarization followed by a rise in [Ca(2+)](in) involving store-operated Ca(2+) entry (SOCE) channels induced myoblast differentiation...
  74. ncbi Cross-talk between Wnt and bone morphogenetic protein 2 (BMP-2) signaling in differentiation pathway of C2C12 myoblasts
    Aiko Nakashima
    Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan
    J Biol Chem 280:37660-8. 2005
    ..These findings identify functional cross-talk of Id1 expression between Wnt and BMP signaling and demonstrate a novel mechanism for Wnt regulation of the BMP-2 response, linking Id1 expression to Wnt/beta-catenin signaling...
  75. pmc High expression of the PAX3-FKHR oncoprotein is required to promote tumorigenesis of human myoblasts
    Shujuan J Xia
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6082, USA
    Am J Pathol 175:2600-8. 2009
    ..between expression and phenotype in human myogenic cells, PAX3-FKHR was introduced into immortalized human myoblasts to produce a low overall PAX3-FKHR expression level...
  76. pmc Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts
    Norio Motohashi
    Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4 1 1 Ogawa Higashi, Kodaira, Tokyo 187 8502, Japan
    Am J Pathol 173:781-91. 2008
    ..clarify the role of these SP cells in muscle regeneration, we injected green fluorescent protein (GFP)-positive myoblasts with or without CD31(-) CD45(-) SP cells into the tibialis anterior muscles of immunodeficient NOD/scid mice or ..
  77. ncbi L- and S-endoglin differentially modulate TGFbeta1 signaling mediated by ALK1 and ALK5 in L6E9 myoblasts
    Soraya Velasco
    Instituto Reina Sofia de Investigacion Nefrologica, Departamento de Fisiologia y Farmacologia, Universidad de Salamanca, and Red de Investigación en Enfermedades Renales RedinRen, Salamanca, Spain
    J Cell Sci 121:913-9. 2008
    ....
  78. pmc Stress-induced C/EBP homology protein (CHOP) represses MyoD transcription to delay myoblast differentiation
    Joel Alter
    Department of Biochemistry, Rappaport Institute for Research in the Medical Sciences, Faculty of Medicine, Technion Israel Institute of Technology, Haifa, Israel
    PLoS ONE 6:e29498. 2011
    When mouse myoblasts or satellite cells differentiate in culture, the expression of myogenic regulatory factor, MyoD, is downregulated in a subset of cells that do not differentiate...
  79. pmc Smad3 signaling is required for satellite cell function and myogenic differentiation of myoblasts
    Xiaojia Ge
    School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore
    Cell Res 21:1591-604. 2011
    ..Further in vitro analysis of primary myoblast cultures identified that Smad3-null myoblasts exhibit impaired proliferation, differentiation and fusion, resulting in the formation of atrophied myotubes...
  80. pmc Beta-catenin interacts with MyoD and regulates its transcription activity
    Chang Hoon Kim
    Program of Developmental Neurobiology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, 1120 15th St, CB2803, Augusta, GA 30912, USA
    Mol Cell Biol 28:2941-51. 2008
    ..These results demonstrate that beta-catenin is necessary for MyoD function, identifying MyoD as an effector in the Wnt canonical pathway...
  81. pmc Role of the RNA-binding protein IMP-2 in muscle cell motility
    Selim Boudoukha
    Universite Paris Sud, Villejuif, France
    Mol Cell Biol 30:5710-25. 2010
    ..that IMP-2, the most ubiquitously expressed member of the family, is abundant in human and mouse adult skeletal myoblasts, where it is indispensable for cell motility and for stabilization of microtubules...
  82. ncbi Role of Annexin A1 in mouse myoblast cell differentiation
    Valentina Bizzarro
    Department of Pharmaceutical Sciences, University of Salerno, Salerno, Italy
    J Cell Physiol 224:757-65. 2010
    ..The data reported here suggest for the first time that ANXA1 plays a role in myogenic differentiation...
  83. ncbi Evidence for fusion between cardiac and skeletal muscle cells
    Hans Reinecke
    Department of Pathology, University of Washington, Box 357470, HSB Room D 514 Seattle, Wash 98195 7470, USA
    Circ Res 94:e56-60. 2004
    Cardiomyoplasty with skeletal myoblasts may benefit cardiac function after infarction. Recent reports indicate that adult stem cells can fuse with other cell types...
  84. ncbi Human muscle cells express a B7-related molecule, B7-H1, with strong negative immune regulatory potential: a novel mechanism of counterbalancing the immune attack in idiopathic inflammatory myopathies
    Heinz Wiendl
    Department of Neurology, University of Tubingen, Medical School, D 72076 Tubingen, Germany
    FASEB J 17:1892-4. 2003
    ..Here we report that human myoblasts cultured from control subjects and patients with inflammatory myopathies as well as TE671 muscle ..
  85. ncbi Expression of serum amyloid A in chondrocytes and myoblasts differentiation and inflammation: possible role in cholesterol homeostasis
    Barbara Zerega
    Istituto Nazionale per la Ricerca sul Cancro Genova, Italy
    Matrix Biol 23:35-46. 2004
    ..In the present study, we identified SAA as a protein expressed by chondrocytes and myoblasts in response to inflammatory stimula...
  86. pmc Valosin containing protein associated inclusion body myopathy: abnormal vacuolization, autophagy and cell fusion in myoblasts
    Jouni Vesa
    Department of Pediatrics, Division of Genetics and Metabolism, University of California, Irvine, CA 92868, USA
    Neuromuscul Disord 19:766-72. 2009
    ..underlying the myopathy in IBMPFD, we have studied the cellular consequences of VCP mutations in human primary myoblasts. Our results revealed that patients' myoblasts accumulate large vacuoles...
  87. ncbi Facioscapulohumeral muscular dystrophy (FSHD) myoblasts demonstrate increased susceptibility to oxidative stress
    Sara T Winokur
    Department of Biological Chemistry, 240D, Medical Sciences I, University of California, Irvine, CA 92697, USA
    Neuromuscul Disord 13:322-33. 2003
    ..However, the 'vacuolar/necrotic' phenotype exhibited by facioscapulohumeral muscular dystrophy myoblasts suggests that aberrant gene expression occurs early in facioscapulohumeral muscular dystrophy muscle development...
  88. pmc Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase
    Atsushi Suzuki
    Division of Endocrinology and Metabolism, Department of Developmental Physiology, National Institute for Physiological Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan
    Mol Cell Biol 27:4317-27. 2007
    ..Our data thus suggest that the activation of and changes in the subcellular localization of alpha2AMPK are required for leptin-induced stimulation of fatty acid oxidation and PPARalpha gene expression in muscle cells...
  89. pmc Regulating a master regulator: establishing tissue-specific gene expression in skeletal muscle
    Arif Aziz
    Sprott Center for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, CA
    Epigenetics 5:691-5. 2010
    ..ChIP-Seq analysis has recently revealed that MyoD binds to a large number of genomic loci in differentiating myoblasts, yet only activates transcription at a subset of these genes...
  90. pmc uPA deficiency exacerbates muscular dystrophy in MDX mice
    Monica Suelves
    Program on Differentiation and Cancer, Center for Genomic Regulation, E 08003, Barcelona, Spain
    J Cell Biol 178:1039-51. 2007
    ..These findings underscore the importance of uPA in muscular dystrophy...
  91. pmc Distinct growth hormone receptor signaling modes regulate skeletal muscle development and insulin sensitivity in mice
    Mahendra D Mavalli
    Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Clin Invest 120:4007-20. 2010
    ..These results identify distinct signaling pathways through which GHR regulates skeletal muscle development and modulates nutrient metabolism...
  92. ncbi Demystifying SP cell purification: viability, yield, and phenotype are defined by isolation parameters
    Federica Montanaro
    Howard Hughes Medical Institute, Boston, MA 02115, USA
    Exp Cell Res 298:144-54. 2004
    ....
  93. pmc MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells
    Shuichi Watanabe
    Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Stem Cells 29:505-16. 2011
    ..It remains unclear, however, whether fully committed skeletal muscle progenitor cells (myoblasts) have the potency to undergo reprogramming to develop iPSCs in line with previously reported cases...
  94. ncbi The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation
    Irina Naguibneva
    UPR9079 CNRS Ligue Nationale Contre le Cancer, 7 rue Guy Moquet, 94801 Villejuif Cedex, France
    Nat Cell Biol 8:278-84. 2006
    ..Therefore, miRNAs can be involved in the establishment of a differentiated phenotype - even when they are not expressed in the corresponding fully differentiated tissue...
  95. pmc Normal myoblast fusion requires myoferlin
    Katherine R Doherty
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Development 132:5565-75. 2005
    ..During embryonic muscle growth and regeneration in mature muscle, singly nucleated myoblasts fuse to each other to form myotubes...
  96. pmc Involvement of the p38 mitogen-activated protein kinase alpha, beta, and gamma isoforms in myogenic differentiation
    Haixia Wang
    Department of Biochemistry, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong, China
    Mol Biol Cell 19:1519-28. 2008
    ..e., E2F2, cyclin D3, and WISP1) are found to be required for myogenin expression, which provides a molecular basis to explain why different p38 isoforms are required for myogenic differentiation...
  97. ncbi Sphingosine 1-phosphate regulates myogenic differentiation: a major role for S1P2 receptor
    Chiara Donati
    Dipartimento di Scienze Biochimiche, Universita degli Studi di Firenze, Florence, Italy
    FASEB J 19:449-51. 2005
    In this study a novel biological activity of sphingosine 1-phosphate (S1P) in C2C12 myoblasts was identified...
  98. ncbi Dysfunction of endocytic and autophagic pathways in a lysosomal storage disease
    Tokiko Fukuda
    Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892 1820, USA
    Ann Neurol 59:700-8. 2006
    ....
  99. ncbi Sphingosine 1-phosphate inhibits cell migration in C2C12 myoblasts
    Laura Becciolini
    Dipartimento di Scienze Biochimiche, Universita degli Studi di Firenze, Viale G B Morgagni 50, 50134 Firenze, Italy
    Biochim Biophys Acta 1761:43-51. 2006
    This study shows that sphingosine 1-phosphate (S1P) exerts an anti-migratory action in C2C12 myoblasts by reducing directional cell motility and fully abrogating the chemotactic response to insulin-like growth factor-1...
  100. ncbi Methylglyoxal impairs the insulin signaling pathways independently of the formation of intracellular reactive oxygen species
    Audrey Riboulet-Chavey
    INSERM U145, IFR50, Faculty of Medicine, 06107 Nice Cedex 2, France
    Diabetes 55:1289-99. 2006
    ..By such a mechanism, methylglyoxal may not only induce the debilitating complications of diabetes but may also contribute to the pathophysiology of diabetes in general...
  101. ncbi Increased sensitivity of myoblasts to oxidative stress in amyotrophic lateral sclerosis peripheral tissues
    Lloyd J Bradley
    Department of Clinical Neurosciences, Institute of Neurology, University College London, Rowland Hill Street, London NW3 2PF, England, UK
    Exp Neurol 218:92-7. 2009
    ..respiratory chain function, mitochondrial DNA (mtDNA) integrity, and oxidative stress levels in muscle, myoblasts, fibroblasts and cybrids, from 12 amyotrophic lateral sclerosis (ALS) patients with 28 control samples...

Research Grants78

  1. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
    ..In addition, skeletal myoblasts expressing PRDM16 formed ectopic depots of adipose tissue after subcutaneous transplantation...
  2. Sphingolipid Metabolism in Drosophila Development
    Julie D Saba; Fiscal Year: 2013
    ..Skeletal muscle is also a prime target organ for gene therapy, since engineered myoblasts can be made to fuse with mature muscle, generating a stable hybrid organ within the adult...
  3. Insulin Signaling and the Heart in Diabetes
    EVAN DALE ABEL; Fiscal Year: 2011
    ..by which insulin signaling suppresses cardiomyocyte autophagy in cultured ventricular myocytes or H9C2 myoblasts. These studies will determine the relative contribution of mTOR activation versus phosphorylation of FOXO1 and ..
  4. FUNCTIONAL AND TARGETING POLYMERIC GENE CARRIERS
    Sung Wan Kim; Fiscal Year: 2012
    ..Purified autologous cells that can be re-injected are the obvious choice and primary myoblasts have been the first to be used in clinical post-infarction transplantation...
  5. The Forkhead Transcription Factor, FOXO1, and its Role in Pituitary Gland Develop
    BUFFY SUE ELLSWORTH; Fiscal Year: 2010
    ..Secondly, FOXO1 inhibits proliferation and regulates differentiation and migration of pancreatic 2-cells, myoblasts, adipocytes, and endothelial cells. To test this hypothesis we propose three specific aims: Specific Aim 1...
  6. Targets of a cytidine deaminase required for left-right axis in Xenopus
    Alin Vonica; Fiscal Year: 2010
    ..Preliminary data indicate that XA2 in Xenopus and the mouse homologue A2 in C2C12 myoblasts inhibit nodal-type TGF-[unreadable] signaling...
  7. The role of cMET in satellite cells during muscle regeneration
    MICAH TAUBE WEBSTER; Fiscal Year: 2013
    ..In uninjured muscle, quiescent SCs express c-Met. Upon muscle injury, SCs activate to proliferate and become myoblasts (muscle precursor cells) that accumulate at sites of injury where they fuse to form new muscle fibers...
  8. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..Aim 2 will establish the regulation of CHC22 intracellular recruitment to membranes in HeLa cells, human myoblasts and transfected 3T3-L1 cells to address the hypothesis that CHC22 influences GLUT4 transport in muscle and fat ..
  9. The Role of PGC-1aplha in the Pathogenesis of Myotonic Dystrophy Type 1
    Xiang Fang; Fiscal Year: 2013
    ..in DM1, and determine whether the activity of the ROS producing enzyme NADPH oxidase is up- regulated in DM1 myoblasts and C2C12 cells expressing mutant CUG repeats;and 3) aim 3 will explore the mechanism by which expanded CUG ..
  10. Generation of Wunen/LPP3-based therapy for muscular dystrophy
    Hannele Ruohola Baker; Fiscal Year: 2010
    ..propose that increasing sphingolipid signaling will increase the regeneration potential of muscle stem cells and myoblasts and thereby will improve the efficacy of muscular dystrophy therapy...
  11. Mechanisms of degeneration in the spinal cord and musculoskeletal system in SMA
    SANIYA FAYZULLINA; Fiscal Year: 2013
    ..of abnormal SMN to facilitate repair of DNA double strand breaks by homologous recombination in proliferating myoblasts, rather than a secondary consequence of motor neuron disease...
  12. GENETIC REGULATION OF SKELETAL MUSCLE REPAIR
    Michael A Rudnicki; Fiscal Year: 2013
    ..stem cells and myogenic cells, in activated satellite cells, during cell-cycle progression of proliferating myoblasts, and during terminal differentiation...
  13. Notch and Wnt signaling in protection by nandrolone against disuse atrophy
    Christopher Cardozo; Fiscal Year: 2012
    ..to allow self-renewal, and of producing daughter cells able to commit to the skeletal muscle lineage, form myoblasts, and fuse to form new muscle fibers or contribute to existing ones...
  14. INTERPLAYS BETWEEN THE JAW MESENCHYMAL STEM CELLS AND T LYMPHOCYTES
    Yufang Shi; Fiscal Year: 2010
    ..of differentiating into different lineage cells including osteoblasts, chondrocytes, adipocytes, cardiomyocytes, myoblasts, and neural cells...
  15. Identification of Biomechanically Valid Cell Therapeutics for Vocal Fold Scar
    Rebecca S Bartlett; Fiscal Year: 2013
    ..not trigger transformation to harmful mesenchymal stromal cell derivatives, such as osteoblasts, chondroctyes or myoblasts. In Specific Aim 2, we will examine mechanically mediated MSC differentiation as a function of time...
  16. IDENTIFICATION OF GENES ASSOCIATED WITH MYOBLAST FUSION
    Susan M Abmayr; Fiscal Year: 2012
    ..the process of myogenesis, with a specific emphasis on the migration, recognition, adhesion and fusion between myoblasts to form multinucleate myotubes...
  17. Age-Dependent Regulation of Muscle Stem Cell Homeostasis
    Bradley B Olwin; Fiscal Year: 2013
    ..cells are generated from a hierarchical stem cell that under- goes asymmetric division to generated committed myoblasts and self-renew and expands by symmetric division or are generated stochastically by a common pool of equipotent ..
  18. Nanion Syncro Patch 96
    Gordana Vunjak-Novakovic; Fiscal Year: 2012
    ..by applicant): Electrophysiological studies of electrically excitable cells, such as cardiac myocytes, skeletal myoblasts, neurons, osteoblasts, are critically important for the derivation of these cells from various stem cell sources ..
  19. Noninvasive Monitoring of In Situ Oxygenation and Cell Therapy in Infarct Heart
    Periannan Kuppusamy; Fiscal Year: 2010
    ..Transplantation of immature cells, such as stem cells or myoblasts, in the infarct area of the heart, known as cell therapy, appears to be a promising alternative strategy...
  20. Molecular Regulation of Myogenic Differentiation
    Thomas A Rando; Fiscal Year: 2013
    ..the pattern of expression and cellular localization of Deltex proteins in myogenic differentiation of primary myoblasts and C2C12 myoblasts...
  21. UFCC for Cardiovascular Cell Therapy Research Network
    Carl J Pepine; Fiscal Year: 2011
    ..Specific aims test whether skeletal myoblasts'(SkM) effect on ventricular function will be dependent on the presence of cells and not a reaction to the ..
  22. ACTIVATORS OF MUSCLE GENES
    Helen M Blau; Fiscal Year: 2013
    ..MuSCs readily give rise in culture to abundant myogenic progenitors called myoblasts, but these cells have extremely limited regenerative potential upon transplantation, as evidenced by failures in ..
  23. Regulation & Function of Calponin
    Jian Ping Jin; Fiscal Year: 2010
    ..JBC, 280:42442-53, 2005) and have extended this study to other cell types, including lung alveolar cells, myoblasts, macrophages, and cancer cells...
  24. Nuclear lamina in regulation of signaling in muscle
    LARRY R GERACE; Fiscal Year: 2013
    ..The work will use cultured myoblasts to analyze how these INM proteins control signaling at the molecular level, and mouse models to understand their ..
  25. Physical and Functional Interactions of Cis Regulatory Modules
    Martha L Bulyk; Fiscal Year: 2010
    ..In this project, as our model system we will examine primary human skeletal muscle myoblasts differentiating into myotubes...
  26. SV40 TUMOR ANTIGEN
    ROBERT TSE NAN TJIAN; Fiscal Year: 2013
    ..Most striking was our finding that during skeletal muscle differentiation (myoblasts^myotubes) there is a wholesale loss of the prototypic TBP-TAF complex (TFIID) and its replacement by a novel ..
  27. Molecular mechanisms regulating motility and migration of muscle satellite cells
    DAWN D CORNELISON; Fiscal Year: 2010
    ..are required to respond by quickly becoming `activated', multiplying to produce a large pool of replacement myoblasts, relocating to the site of the injury, and differentiating to form new muscle fibers...
  28. Regulation of Skeletal Muscle Regeneration
    Yi Ping Li; Fiscal Year: 2012
    ..We will test the conceptual model in cultured myoblasts and adult muscle that as an endogenous inhibitor of TACE, constitutively expressed TIMP3 suppresses myogenic ..
  29. TAK1/TRAF6 Signaling in Skeletal Muscle
    Ashok Kumar; Fiscal Year: 2013
    ..1) Investigate the signaling mechanisms by which TAK1 and TRAF6 regulate myogenic differentiation in cultured myoblasts;2) Investigate the role and cellular mechanisms by which TAK1 and TRAF6 regulate skeletal muscle development in ..
  30. The role of SIK1 in myogenic differentiation and skeletal muscle repair
    Rebecca L Berdeaux; Fiscal Year: 2013
    ..However, little is known about how the enzyme itself is regulated in undifferentiated myoblasts, whether its function is required during muscle development, or whether Sik1 deletion in myofibers will cause ..
  31. Metabolic Characterization of the Novel Adipokine CTRP3
    Jonathan Peterson; Fiscal Year: 2011
    ..protein, I showed that CTRP3 potentiates the action of insulin in activating the Akt signaling pathway in mouse myoblasts, and furthermore injection of recombinant CTRP3 into mice significantly lowered their blood glucose levels...
  32. Satellite Cell Development and Potential
    David J Goldhamer; Fiscal Year: 2010
    ..To investigate the competing hypotheses that satellite cells are derived from fetal myoblasts and cells of the vasculature (e.g...
  33. INSULIN-LIKE GROWTH FACTORS AND MUSCLE DIFFERENTIATION
    PETER S ROTWEIN; Fiscal Year: 2012
    ..The first key step in activating this network is production and secretion of IGF-II by differentiating myoblasts, which is secondary to induction of IGF-II gene transcription...
  34. Electrophysiological and antiarrhythmic benefit of cell therapy for heart disease
    KENNETH LAURITA; Fiscal Year: 2010
    ..studies suggest that cardiac function can be restored by adding new, healthy cells such as skeletal muscle myoblasts (SKMB) or bone marrow-derived mesenchymal stem cells (MSC)...
  35. Soluble Motogens and Chemoattractants from Damaged Muscle
    DAWN D CORNELISON; Fiscal Year: 2013
    ..is damaged they will activate from their usual quiescent state and proliferate to form a pool of replacement myoblasts, which will differentiate into new muscle fibers or fuse with damaged ones to rapidly and effectively repair the ..
  36. Mechanism of regulation for the oncogenic Pax3-FOXO1 in Alveolar Rhabdomyosarcoma
    ANDREW DURRELL HOLLENBACH; Fiscal Year: 2013
    ..to Pax3 DNA binding ability, Pax3 and Pax3-FOXO1 are phosphorylated at Ser205 in proliferating primary myoblasts, and that this phosphorylation is rapidly lost on Pax3 but not Pax3-FOXO1 upon the induction of myogenic ..
  37. Molecular Mechanism of Akirin Function During Myogenesis
    SCOTT JAMES NOWAK; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The specification and differentiation of myoblasts by the action of myogenic transcription factors is a key event in the formation of the skeletal musculature...
  38. Rheumatic Disease Sera: Probes of Disease Mechanisms
    Livia A Casciola-Rosen; Fiscal Year: 2012
    ..from myositis patients specifically recognize previously undefined autoantigens expressed exclusively in myoblasts or differentiating muscle cells, and that cytotoxic lymphocytes cluster around such cells in myositis muscle...
  39. Preconditioning and re-programming of stem cells for cardiac repair
    Khawaja H Haider; Fiscal Year: 2012
    ..In order to achieve these effects the present study involves transplantation of BMCs together with skeletal myoblasts (SkMs) which will serve mainly as carries of therapeutic genes...
  40. Pathogenesis of Myopathy in Models of Myotonic Dystrophy
    Charles Thornton; Fiscal Year: 2009
    ..In related experiments we will investigate the mechanism of cell death that occurs in HSA(LR) myoblasts when growth factors are withdrawn...
  41. High Content Screening for Muscular Dystrophy
    Herman H Vandenburgh; Fiscal Year: 2010
    ..In Myomics'Phase I SBIR project mBAMs were tissue engineered from conditionally immortalized myoblasts from the mdx mouse, the small animal model of DMD...
  42. 3D Bioengineering Strategies to Mimic Human Skeletal Muscle Progenitor Cell Niche
    Sarah C Heilshorn; Fiscal Year: 2012
    ..Aim 3 is to develop a 3D patterned mimic of human skeletal muscle tissue. Human myoblasts will be cultured on patterned eECM to induce myotube fusion and alignment...
  43. Mitochondrial sites of reactive oxygen species generation in cells.
    Martin D Brand; Fiscal Year: 2010
    ..and experimentally-amenable cells (including neurons, astrocytes, normal and senescent fibroblasts, C2C12 myoblasts, and pancreatic insulinoma cells oxidizing physiological substrates in different states...
  44. Amphibian Models for Bodywall Patterning
    Ann Campbell Burke; Fiscal Year: 2010
    ..The primaxial domain is populated exclusively by cells from the somites. The abaxial domain is made up of myoblasts from the somites and connective tissue of the lateral plate in which the muscles differentiate...
  45. 3D combinatorial microenvironments for effective cell based therapy
    Ronglih Liao; Fiscal Year: 2013
    ..this goal, cell-based therapy for cardiac regeneration employing various cell types, ranging from skeletal myoblasts to bone marrow derived stem cells to endogenous cardiac stem/progenitor cells, has sparked tremendous interest ..
  46. The Growth Hormone/IGF-1 Axis in Skeletal Muscle
    Thomas L Clemens; Fiscal Year: 2013
    ..by applicant): Mammalian skeletal muscle develops and regenerates through a process in which individual myoblasts fuse with one another to create a multinucleated syncytium...
  47. Molecular Mechanisms of Myoblast Fusion
    ELIZABETH HUI CHEN; Fiscal Year: 2013
    ..composed of multinucleate muscle fibers, each of which is the product of fusion of hundreds or even thousands of myoblasts. Myoblast fusion is not only important for skeletal muscle development, but also critical for satellite cell-..
  48. THE ROLE OF CDO IN MYOGENIC DIFFERENTIATION
    Robert S Krauss; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Differentiation of vertebrate skeletal myoblasts into multinucleated myofibers is a multistage process that involves the coordinated activation of a cell-type specific transcriptional program and ..
  49. Dissecting the Mechanisms of Cell-Cell Fusion Using A Cell Culture System
    ELIZABETH HUI CHEN; Fiscal Year: 2013
    ..revealed an essential function of the actin cytoskeleton in myoblast fusion, the process in which mononucleate myoblasts fuse to form multinucleate muscle fibers...
  50. Genetically Engineered Muscle Stem Cell Transplantation for Muscular Dystrophy...
    Atsushi Asakura; Fiscal Year: 2013
    ..unlimited number of satellite cells from induced pluripotent stem cells (iPSCs) derived from the patient's myoblasts. In combination with gene transduction, this concerted approach will help us to make satellite cells that can be ..
  51. Integrin Alleviation of Muscular Dystrophy
    Dean J Burkin; Fiscal Year: 2012
    ..The 1721 integrin is a major laminin receptor expressed in satellite cells, myoblasts and vascular smooth muscle...
  52. CaV beta1a as a Transcriptional Regulator of Muscle Regeneration and Sarcopenia
    JACKSON RICHARD TAYLOR; Fiscal Year: 2012
    ..A primary cause of this loss is the diminished ability of muscle-specific stem cells (called myoblasts) to divide and proliferate as needed to repair muscle damage...
  53. High-fat diet rescues lethality of homozygous knock-in R155H VCP myopathic mice
    VIRGINIA EUNICE KIMONIS; Fiscal Year: 2013
    ..Studies of patients'myoblasts and the heterozygous R155H mouse have placed VCP at the intersection of the ubiquitin-proteasome signaling and ..
  54. Myotonic Dystrophy Type 2
    Lubov T Timchenko; Fiscal Year: 2013
    ..Because ZNF9 controls synthesis of proteins of translational apparatus, its reduction in DM2 myoblasts decreases the rate of global protein synthesis...
  55. mRNA synthesis in animal cells - 3'end formation
    James L Manley; Fiscal Year: 2013
    ..The mechanistic basis for this will be investigated with a model in vitro system using C2C12 myoblasts, which will allow identification of the factor(s) involved...
  56. Clonal isogenic and immortalized FSHD myoblasts with or without D4Z4 contraction
    Silvere M van der Maarel; Fiscal Year: 2011
    ..This means that they are genetically identical except for the mutation. We expect that these cell lines will aid the understanding of the disease mechanism and will be useful for small molecule screens for therapeutic purposes. ..
  57. Molecular regulation of skeletal myoblast apoptosis
    CRYSTAL WEYMAN; Fiscal Year: 2009
    Programmed cell death (apoptosis) is induced concomitant with differentiation in skeletal myoblasts and other systems. In skeletal myoblasts, these two processes result in mutually exclusive physiologically important endpoints...
  58. The role of the dermatomyositis autoantigen MI-2 in muscle regeneration
    ANDREW L MAMMEN; Fiscal Year: 2012
    ..Preliminary data from an in vitro myoblast differentiation system show that high Mi-2 levels in proliferating myoblasts are down-regulated during differentiation...
  59. The nucleus-cytoskeleton connection in health and disease
    Denis Wirtz; Fiscal Year: 2011
    ..of Lmna on the LINC complex integrity and cytoskeleton functions will be examined in mouse fibroblasts and myoblasts. We anticipate that this project will shed light on the biophysical principles that govern nucleus dynamics and ..
  60. High Throughput Screen for Myotonic Dystrophy Type 1
    SITA contact REDDY; Fiscal Year: 2011
    ..identification of a potent lead compound, MDI16, which reverses aberrant RNA splice patterns in both DM1 patient myoblasts and in the HSALR mouse model for DM1...
  61. Investigation of Sulf regulation on the noncanonical Wnt signaling pathway
    Thanh Huu Tran; Fiscal Year: 2013
    ..and differentiation of skeletal muscle stem cells known as satellite cells (SC) and the fusion of nascent myoblasts (differentiated SC)...
  62. Translational Studies in VCP Inclusion Body Myopathy with Paget Disease of Bone
    Virginia Kimonis; Fiscal Year: 2009
    ..To clarify the cellular pathology of mutant primary myoblasts from patients Insight into the disease pathogenesis gained by our detailed clinical evaluations, analysis of ..
  63. DEVELOPING CELLULAR CARDIOMYOPLASTY FOR INJURED HEART
    DORIS TAYLOR; Fiscal Year: 2001
    ..myocardial performance can be at least partly reversed by repopulating the damaged area with primary skeletal myoblasts to re- form a functional unit within an infarct region...
  64. SKI PROTO-ONCOGENE FAMILY IN EARLY DEVELOPMENT
    Sonia Pearson White; Fiscal Year: 1992
    ..Members of the ski family of genes, ski and sno, are expressed during skeletal muscle development, in determined myoblasts and in differentiated myofibers, and at lower levels in adult muscle...
  65. MYOBLAST GROWTH AND DIFFERENTIATION DURING MUSCLE AGING
    Charlotte Peterson; Fiscal Year: 2000
    ..Muscle damage is repaired directly and through recruitment of satellite cells, myoblasts that proliferate and differentiate in response to muscle degeneration thereby regenerating damaged muscle fibers...
  66. SK CHANNELS IN HYPEREXCITABLE SKELETAL MUSCLE
    John Adelman; Fiscal Year: 2001
    ..3). The SK3 promotor is activated following differentiation of cultured L6 myoblasts. a) SK3 promotor/luciferase constructs will be introduced into L6 myoblasts, and luciferase activity assessed ..
  67. POSTEMBRYONIC DEVELOPMENT OF LEG MOTOR CIRCUITS
    Richard Levine; Fiscal Year: 2000
    ..The myoblasts that generate the adult muscles accumulate around the re-expanding motor terminals, then proliferate, fuse and ..
  68. HISTOINCOMPATIBLE MYOBLAST INJECTION AGAINST DYSTROPHY
    Peter Law; Fiscal Year: 1992
    Implantation of normal myoblasts/fibroblasts (co-culture) into dystrophic solei of histocompatible mice improved the structure and functions of the muscles to almost normal...
  69. MUSCLE CELLS MEDIATED GENE THERAPY FOR INCONTINENCE
    Michael Chancellor; Fiscal Year: 2005
    ..We have evidence that bladder injection of MDSC rather than myoblasts persist in the bladder up to 6 months. MDSC can differentiate into smooth muscle...
  70. Cellular Cardiomyoplasty in Vitro: Host-Donor Cell Interactions
    Nenad Bursac; Fiscal Year: 2007
    ..While initial clinical implantations of autologous skeletal myoblasts and bone marrow cells have been promising, the intrinsic potential of these cells to affect electro-mechanical ..
  71. Mechanical Stimulation of 2-and 3-d Myoblast Culture
    GEORGE TRUSKEY; Fiscal Year: 2006
    Skeletal myoblasts are a population of progenitor cells in adult skeletal muscle that are capable of differentiating intovarious types of skeletal muscle...
  72. FIBROBLAST GROWTH FACTORS AND STRIATED MUSCLE
    JUDITH SWAIN; Fiscal Year: 1993
    ..It has recently been demonstrated that overexpression of an FGF delays the differentiation of skeletal myoblasts, while inhibition of FGF expression by antisense techniques triggers myogenesis...
  73. FIBROBLAST GROWTH FACTORS AND STRIATED MUSCLE
    JUDITH SWAIN; Fiscal Year: 2001
    ..It has recently been demonstrated that overexpression of an FGF delays the differentiation of skeletal myoblasts, while inhibition of FGF expression by antisense techniques triggers myogenesis...
  74. CELL LINEAGE COMMITMENT IN MAMMALIAN MYOGENESIS
    Jeffrey Miller; Fiscal Year: 1991
    ..Multiple types of myoblasts, as well as multiple types of muscle fibers, are found at the different stages of myogenesis in the mouse...
  75. REGULATION OF EXPRESSION OF CREATINE KINASE GENE FAMILY
    Joseph Billadello; Fiscal Year: 1992
    ..for the tissue-specific and developmental regulation of the M creatine kinase gene in transfected C(2)C(12) myoblasts undergoing differentiation in culture...
  76. Immune Tolerence to Transplanted Myoblasts
    David M Rothstein; Fiscal Year: 2010
    ..This will require the induction of immunological tolerance towards allogeneic myoblasts, as well as to neoantigens resulting from the fusion of donor myoblasts with host muscle fibers (including ..
  77. BIOARTIFICIAL MUSCLES FOR GENE THERAPY
    Herman Vandenburgh; Fiscal Year: 2002
    ..Muscle stem cells (myoblasts) can be isolated by simple needle biopsy and genetically modified to express foreign proteins...
  78. ATTENUATION OF MUSCLE WASTING WITH GROWTH HORMONE
    Herman Vandenburgh; Fiscal Year: 2000
    ..Cell Based Delivery, Inc. holds the exclusive licensing rights to technology whereby genetically modified myoblasts are tissue engineered into organized bioartificial skeletal muscles (BAMs) which can be implanted for the long-..