erythroid cells

Summary

Summary: The series of cells in the red blood cell lineage at various stages of differentiation.

Top Publications

  1. pmc ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis
    Anna H Schuh
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Mol Cell Biol 25:10235-50. 2005
  2. pmc The miR-144/451 locus is required for erythroid homeostasis
    Kasper D Rasmussen
    European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy
    J Exp Med 207:1351-8. 2010
  3. pmc Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells
    Stefan Schoenfelder
    Laboratory of Chromatin and Gene Expression, Babraham Research Campus, Cambridge, UK
    Nat Genet 42:53-61. 2010
  4. ncbi Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A
    Vijay G Sankaran
    Division of Hematology Oncology, Children s Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 322:1839-42. 2008
  5. pmc Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo
    M Mortensen
    Nuffield Department of Medicine, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 107:832-7. 2010
  6. pmc The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation
    Eric Soler
    Department of Cell Biology, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands
    Genes Dev 24:277-89. 2010
  7. pmc Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6
    Jian Xu
    Children s Hospital Boston, Massachusetts 02115, USA
    Genes Dev 24:783-98. 2010
  8. pmc Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells
    Mira T Kassouf
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford University, Oxford OX3 9DS, United Kingdom
    Genome Res 20:1064-83. 2010
  9. pmc Nuclear receptors TR2 and TR4 recruit multiple epigenetic transcriptional corepressors that associate specifically with the embryonic β-type globin promoters in differentiated adult erythroid cells
    Shuaiying Cui
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Mol Cell Biol 31:3298-311. 2011
  10. pmc A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells
    Michael R Tallack
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Genome Res 20:1052-63. 2010

Detail Information

Publications247 found, 100 shown here

  1. pmc ETO-2 associates with SCL in erythroid cells and megakaryocytes and provides repressor functions in erythropoiesis
    Anna H Schuh
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Mol Cell Biol 25:10235-50. 2005
    ..Genetic studies of erythroid cells show that ETO-2 exerts a repressor effect on SCL target genes...
  2. pmc The miR-144/451 locus is required for erythroid homeostasis
    Kasper D Rasmussen
    European Molecular Biology Laboratory, Mouse Biology Unit, Monterotondo Scalo, 00015, Italy
    J Exp Med 207:1351-8. 2010
    ..Thus, the miR-144/451 cluster tunes gene expression to impart a robustness to erythropoiesis that is critical under conditions of stress...
  3. pmc Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells
    Stefan Schoenfelder
    Laboratory of Chromatin and Gene Expression, Babraham Research Campus, Cambridge, UK
    Nat Genet 42:53-61. 2010
    ..Our results establish a new gene expression paradigm, implying that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hot spots optimized for efficient and coordinated transcriptional control...
  4. ncbi Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A
    Vijay G Sankaran
    Division of Hematology Oncology, Children s Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 322:1839-42. 2008
    ..Moreover, abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells. Down-regulation of BCL11A expression in primary adult erythroid cells leads to robust HbF expression...
  5. pmc Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo
    M Mortensen
    Nuffield Department of Medicine, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom
    Proc Natl Acad Sci U S A 107:832-7. 2010
    ..In summary, we show that the selective removal of mitochondria by autophagy, but not other organelles, during erythropoeisis is essential and that this is a necessary developmental step in erythroid cells.
  6. pmc The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation
    Eric Soler
    Department of Cell Biology, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands
    Genes Dev 24:277-89. 2010
    ..This establishes the Ldb1 complex as a positive regulator of the final steps of erythroid differentiation that acts through the shedding of negative regulators and the active interaction between regulatory sequences...
  7. pmc Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6
    Jian Xu
    Children s Hospital Boston, Massachusetts 02115, USA
    Genes Dev 24:783-98. 2010
    ..Our findings provide insight into the mechanism of BCL11A action and new clues for the developmental gene regulatory programs that function at the beta-globin locus...
  8. pmc Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells
    Mira T Kassouf
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford University, Oxford OX3 9DS, United Kingdom
    Genome Res 20:1064-83. 2010
    ..action in erythropoiesis, we performed ChIP-sequencing and gene expression analyses from primary fetal liver erythroid cells. We show that TAL1 coordinates expression of genes in most known red cell-specific processes...
  9. pmc Nuclear receptors TR2 and TR4 recruit multiple epigenetic transcriptional corepressors that associate specifically with the embryonic β-type globin promoters in differentiated adult erythroid cells
    Shuaiying Cui
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Mol Cell Biol 31:3298-311. 2011
    ..By chromatin immunoprecipitation (ChIP) we show that, in adult erythroid cells, TR2/TR4 bind to the embryonic β-type globin promoters but not to the adult β-globin promoter...
  10. pmc A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells
    Michael R Tallack
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    Genome Res 20:1052-63. 2010
    ..We found at least 945 sites in the genome of E14.5 fetal liver erythroid cells which are occupied by endogenous KLF1...
  11. pmc Generation of erythroid cells from fibroblasts and cancer cells in vitro and in vivo
    Shiwu Zhang
    Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Lett 333:205-12. 2013
    Bone marrow is generally considered the main source of erythroid cells. Here we report that a single hypoxia-mimic chemical, CoCl2, can increase the size of fibroblasts and cancer cells and lead to formation of polyploidy giant cells (..
  12. pmc Erythroid Krüppel-like factor directly activates the basic Krüppel-like factor gene in erythroid cells
    Alister P W Funnell
    School of Molecular and Microbial Biosciences, G08, University of Sydney, Sydney, NSW 2006, Australia
    Mol Cell Biol 27:2777-90. 2007
    ..Interestingly, both the ubiquitous and the erythroid promoter are dependent on Eklf in erythroid cells. Eklf also activates both promoters in transient assays...
  13. pmc Chromatin occupancy analysis reveals genome-wide GATA factor switching during hematopoiesis
    Louis C Dore
    Division of Hematology Oncology, Northwestern University, Chicago, IL, USA
    Blood 119:3724-33. 2012
    ..Finally, by comparing GATA1 occupancy in erythroid cells and megakaryocytes, we find that the presence of ETS factor motifs is a major discriminator of megakaryocyte ..
  14. pmc NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development
    Annarita Miccio
    Division of Hematology, The Children s Hospital of Philadelphia, Philadelphia, PA, USA
    EMBO J 29:442-56. 2010
    ..Gene expression analysis in primary mutant erythroid cells and megakaryocytes (MKs) revealed an essential function for NuRD during both the repression and activation of ..
  15. ncbi Epigenetic regulation of hematopoietic differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1
    Shireen Saleque
    Division of Hematology Oncology, Children s Hospital Boston, Boston, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 27:562-72. 2007
    ..Lineage-restricted deployment of these cofactors through interaction with Gfi proteins controls hematopoietic differentiation...
  16. pmc AUF-1 and YB-1 are critical determinants of β-globin mRNA expression in erythroid cells
    Sebastiaan van Zalen
    Department of Medicine Hematology Oncology, Perelman School of Medicine, University of Pennsylvania, and The Children s Hospital of Philadelphia, 3615 Civic Center Blvd, Philadelphia, PA 19104, USA
    Blood 119:1045-53. 2012
    The normal accumulation of β-globin protein in terminally differentiating erythroid cells is critically dependent on the high stability of its encoding mRNA. The molecular basis for this property, though, is incompletely understood...
  17. pmc Regulation of divalent metal transporter 1 (DMT1) non-IRE isoform by the microRNA Let-7d in erythroid cells
    Immacolata Andolfo
    CEINGE, Biotecnologie Avanzate, Via, Comunale Margherita 482, 80145 Naples, Italy
    Haematologica 95:1244-52. 2010
    ..DMT1-IRE might be the major DMT1 isoform expressed in erythroid cells, although its regulation pathways are still unknown.
  18. pmc Hydroxyurea nitrosylates and activates soluble guanylyl cyclase in human erythroid cells
    Vladan P Cokic
    Laboratory of Experimental Hematology, Institute for Medical Research, Belgrade, Serbia
    Blood 111:1117-23. 2008
    ..These data add to an expanding appreciation of the role of hydroxyurea as an inducer of the NO/cGMP pathway in EPCs. These mechanisms may also be involved in the cytostatic effects of hydroxyurea, as well as the induction of HbF...
  19. pmc Essential role for Nix in autophagic maturation of erythroid cells
    Hector Sandoval
    Department of Immunology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 454:232-5. 2008
    b>Erythroid cells undergo enucleation and the removal of organelles during terminal differentiation...
  20. ncbi Common fragile site profiling in epithelial and erythroid cells reveals that most recurrent cancer deletions lie in fragile sites hosting large genes
    Benoît Le Tallec
    Institut Curie, Centre de Recherche, 26 Rue d Ulm, 75248 Paris, France
    Cell Rep 4:420-8. 2013
    ..We further demonstrate that, like CFSs, cancer deletions are significantly enriched in genes over 300 kb long. We now provide evidence that over 50% of recurrent cancer deletions originate from CFSs associated with large genes...
  21. ncbi Expression of miR-210 during erythroid differentiation and induction of gamma-globin gene expression
    Nicoletta Bianchi
    BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara, 74 44100 Ferrara, Italy
    BMB Rep 42:493-9. 2009
    ..Altogether, the data suggest that miR-210 might be involved in increased expression of gamma-globin genes in differentiating erythroid cells.
  22. pmc Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection
    Shokrollah Elahi
    Division of Infectious Diseases and Perinatal Institute, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Nature 504:158-62. 2013
    ..Here we show that physiologically enriched CD71(+) erythroid cells in neonatal mice and human cord blood have distinctive immunosuppressive properties...
  23. ncbi An erythroid differentiation-specific splicing switch in protein 4.1R mediated by the interaction of SF2/ASF with an exonic splicing enhancer
    Guang Yang
    D610, 44 Binney Street, Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Blood 105:2146-53. 2005
    ....
  24. pmc A positive role for NLI/Ldb1 in long-range beta-globin locus control region function
    Sang Hyun Song
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 28:810-22. 2007
    ....
  25. ncbi Regulation of the human HBA genes by KLF4 in erythroid cell lines
    M Giuseppina Marini
    Istituto di Neurogenetica e Neurofarmacologia, Consiglio Nazionale delle Ricerche, Cagliari, Italy
    Br J Haematol 149:748-58. 2010
    ..All experiments combined support a regulatory role of KLF4 in the control of HBA gene expression...
  26. pmc Epigenetic and molecular profiles of erythroid cells after hydroxyurea treatment in sickle cell anemia
    Aisha L Walker
    Department of Pharmaceutical Sciences, St Jude Children s Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA
    Blood 118:5664-70. 2011
    ..This study was registered at ClinicalTrials.gov (NCT00305175)...
  27. pmc C(5) modified uracil derivatives showing antiproliferative and erythroid differentiation inducing activities on human chronic myelogenous leukemia K562 cells
    Eleonora Brognara
    BioPharmaNet, Department of Biochemistry and Molecular Biology, Molecular Biology Division, University of Ferrara, Via Luigi Borsari, 46 44100 Ferrara, Italy
    Eur J Pharmacol 672:30-7. 2011
    ....
  28. pmc Transcriptome profiling and sequencing of differentiated human hematopoietic stem cells reveal lineage-specific expression and alternative splicing of genes
    Poching Liu
    Genomics Core Facility, Genetics and Development Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Physiol Genomics 43:1117-34. 2011
    ..Knowledge of molecular processes by which the cells acquire their cell-specific fate would be beneficial in developing cell-based therapies for human diseases...
  29. ncbi Gfi1b:green fluorescent protein knock-in mice reveal a dynamic expression pattern of Gfi1b during hematopoiesis that is largely complementary to Gfi1
    Lothar Vassen
    Institut für Zellbiologie Tumorforschung, Universitatsklinikum Essen, Germany
    Blood 109:2356-64. 2007
    ..We found highly dynamic expression patterns of Gfi1b in erythroid cells, megakaryocytes, and their progenitor cells (MEPS) where Gfi1 is not detected...
  30. pmc Induction of human fetal hemoglobin via the NRF2 antioxidant response signaling pathway
    Elizabeth R Macari
    Department of Pharmacology and Toxicology
    Blood 117:5987-97. 2011
    ..siRNA knockdown of NRF2 inhibited γ-globin induction by tBHQ. When tested in human primary erythroid cells, tBHQ induced NRF2 binding to the γ-globin promoter, increased γ-globin mRNA and HbF, and suppressed β-..
  31. pmc Distinct Ldb1/NLI complexes orchestrate γ-globin repression and reactivation through ETO2 in human adult erythroid cells
    Christine M Kiefer
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 118:6200-8. 2011
    ..mediates long-range interaction between the β-globin locus control region (LCR) and gene in adult mouse erythroid cells, but whether this complex mediates chromatin interactions at other developmental stages or in human cells is ..
  32. pmc Activator-mediated recruitment of the MLL2 methyltransferase complex to the beta-globin locus
    Celina Demers
    Sprott Center for Stem Cell Research, Ottawa Health Research Institute, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada
    Mol Cell 27:573-84. 2007
    ..Here, we show that the MLL2 complex associates with the hematopoietic activator NF-E2 in erythroid cells and is important for H3K4 trimethylation and maximal levels of transcription at the beta-globin locus...
  33. pmc Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA
    Yuichi Otsuka
    Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210 1218, USA
    Mol Cell Biol 29:2155-67. 2009
    Endonuclease decay of nonsense-containing beta-globin mRNA in erythroid cells generates 5'-truncated products that were reported previously to have a cap or caplike structure...
  34. ncbi In vitro clinical-grade generation of red blood cells from human umbilical cord blood CD34+ cells
    Eun Jung Baek
    The Department of Laboratory Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea
    Transfusion 48:2235-45. 2008
    ....
  35. ncbi BMP4 and Madh5 regulate the erythroid response to acute anemia
    Laurie E Lenox
    Department of Veterinary Science, Graduate Program in Biochemistry, Microbiology and Molecular Biology, and Huck Institute for Life Sciences Cell and Developmental Biology Option, Pennsylvania State University, University Park, PA 16802, USA
    Blood 105:2741-8. 2005
    ..These findings suggest a new model where stress erythroid progenitors, resident in the spleen, are poised to respond to changes in the microenvironment induced by acute anemia...
  36. pmc Actual ligation frequencies in the chromosome conformation capture procedure
    Alexey A Gavrilov
    Institute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia
    PLoS ONE 8:e60403. 2013
    ..Here, using the mouse beta-globin genes in erythroid cells as a model, we estimated the actual frequencies of ligation between the fragments bearing the promoter of the ..
  37. ncbi ERK1/2 inactivation and p38 MAPK-dependent caspase activation during guanosine 5'-triphosphate-mediated terminal erythroid differentiation of K562 cells
    Mohammad Amin Moosavi
    Institute of Biochemistry and Biophysics, University of Tehran, P O Box 13145 1384, Tehran, Iran
    Int J Biochem Cell Biol 39:1685-97. 2007
    ....
  38. ncbi The miR-144/451eGFP allele, a novel tool for resolving the erythroid potential of hematopoietic precursors
    Kasper D Rasmussen
    European Molecular Biology Laboratory, Monterotondo Scalo, Italy
    Blood 118:2988-92. 2011
    ..Using competitive reconstitution experiments in the Ly5 transplant model, we show that eGFP linearly correlates with Ly5 expression. Thus, the miR-144/451(eGFP) allele represents a novel tool for the resolution of erythroid potential...
  39. pmc Methylation of histone H4 by arginine methyltransferase PRMT1 is essential in vivo for many subsequent histone modifications
    Suming Huang
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Genes Dev 19:1885-93. 2005
    ..Consistent with earlier reports of experiments in vitro, H4 Arg3 methylation by PRMT1 appears to be essential in vivo for the establishment or maintenance of a wide range of "active" chromatin modifications...
  40. pmc Correction of sickle cell disease in adult mice by interference with fetal hemoglobin silencing
    Jian Xu
    Division of Hematology Oncology, Children s Hospital Boston and Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 334:993-6. 2011
    ..Thus, interference with HbF silencing by manipulation of a single target protein is sufficient to reverse SCD...
  41. ncbi Erythroid cells in vitro: from developmental biology to blood transfusion products
    Anna Rita Migliaccio
    Tisch Cancer Institute, Mount Sinai School of Medicine, ew York, NY 10029, USA
    Curr Opin Hematol 16:259-68. 2009
    ..These considerations highlight the importance of developing alternative blood products...
  42. pmc miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2
    Luke Pase
    Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
    Blood 113:1794-804. 2009
    ..These studies define a new genetic pathway promoting erythroid maturation (mnr/miR-451/gata2) and provide a rare example of partial rescue of a mutant phenotype solely by miRNA overexpression...
  43. pmc An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis
    Xiang Yu
    Cell and Molecular Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    J Clin Invest 117:1856-65. 2007
    ..Our findings illustrate what we believe to be a novel adaptive mechanism by which a specialized cell coordinates high-level production of a multisubunit protein and protects against various synthetic imbalances...
  44. pmc Regulated Fox-2 isoform expression mediates protein 4.1R splicing during erythroid differentiation
    Guang Yang
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    Blood 111:392-401. 2008
    ..b>Erythroid cells express 2 UGCAUG-binding mFox-2 isoforms, an erythroid differentiation-inducible mFox-2A and a commonly ..
  45. pmc MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis
    Nadia Felli
    Dept of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy
    Haematologica 94:479-86. 2009
    ..This study focused on the functional role of microRNA-223 (miR-223) on erythroid differentiation...
  46. pmc A transient definitive erythroid lineage with unique regulation of the β-globin locus in the mammalian embryo
    Kathleen E McGrath
    Department of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester Medical Center, Rochester, NY, USA
    Blood 117:4600-8. 2011
    ..We conclude that yolk sac-derived EMPs, the first of 2 origins of definitive erythropoiesis, express a unique pattern of globin genes as they generate the first definitive erythrocytes in the liver of the mammalian embryo...
  47. ncbi GATA factor switching during erythroid differentiation
    Hiroshi Kaneko
    Department of Medical Biochemistry, Tohoku University Graduate School of Medicine, 2 1 Seiryo cho, Aoba ku, Sendai, Japan
    Curr Opin Hematol 17:163-8. 2010
    ..clarified that lineage-specific transcription factor networks are essential for proper differentiation of erythroid cells. The transcription factors GATA1 and GATA2 are involved in such networks that regulate erythroid gene ..
  48. pmc Characterization of the transcriptome profiles related to globin gene switching during in vitro erythroid maturation
    Biaoru Li
    Department Pediatrics, Georgia Health Sciences University, 1120 15th St, CN 4112, Augusta, GA 30912, USA
    BMC Genomics 13:153. 2012
    ....
  49. pmc Cyclin E phosphorylation regulates cell proliferation in hematopoietic and epithelial lineages in vivo
    Alex C Minella
    Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Genes Dev 22:1677-89. 2008
    ..Overall these data show the critical role of both degrons in regulating cyclin E activity and reveal that complete loss of Fbw7-mediated cyclin E degradation causes spontaneous and cell type-specific proliferative anomalies...
  50. pmc Hydroxyurea generates nitric oxide in human erythroid cells: mechanisms for gamma-globin gene activation
    Tzu Fang Lou
    University of Texas at Dallas, Department of Molecular and Cell Biology, Richardson, Texas 75080, USA
    Exp Biol Med (Maywood) 234:1374-82. 2009
    ..However, the direct measurement of NO in erythroid cells and its role in fetal hemoglobin induction have not been defined...
  51. pmc A comprehensive analysis of GATA-1-regulated miRNAs reveals miR-23a to be a positive modulator of erythropoiesis
    Yong Zhu
    National Laboratory of Medical Molecular Biology, Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences CAMS and Peking Union Medical College PUMC, Beijing 100005, PR China
    Nucleic Acids Res 41:4129-43. 2013
    ..in mice or zebrafish demonstrated that it was essential for normal morphology in terminally differentiated erythroid cells. Furthermore, a protein tyrosine phosphatase, SHP2, was identified as a downstream target of miR-23a that ..
  52. ncbi Role of transcriptional corepressor ETO2 in erythroid cells
    Tohru Fujiwara
    Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan
    Exp Hematol 41:303-15.e1. 2013
    ..In summary, ETO2 regulates GATA-1 target genes critical for erythroid differentiation, and the decrease of ETO2 levels during erythroid differentiation would contribute to the activation of these targets...
  53. ncbi Induction of signalling in non-erythroid cells by pharmacological levels of erythropoietin
    E A Dunlop
    Centre for Cancer Research and Cell Biology, Queens University Belfast, and Haematology Department, Belfast City Hospital, UK
    Neurodegener Dis 3:94-100. 2006
    ..The increased Epo-induced signalling was not associated with a growth advantage for the NSCLC cells...
  54. ncbi MicroRNA-223 reversibly regulates erythroid and megakaryocytic differentiation of K562 cells
    Jin Yun Yuan
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Beijing, People s Republic of China
    J Cell Mol Med 13:4551-9. 2009
    ..MiR-223 was detected not only in granulocytes but also in erythroid cells. In K562 cells, expression of miR-223 was down-regulated during haemin-induced erythroid differentiation but ..
  55. pmc Role of helix-loop-helix proteins during differentiation of erythroid cells
    Archana Anantharaman
    Department of Biochemistry and Molecular Biology, College of Medicine, Genetics Institute, Center for Epigenetics, Shands Cancer Center, University of Florida, Gainesville, FL 32610 0245, USA
    Mol Cell Biol 31:1332-43. 2011
    ..Among the HLH proteins expressed in differentiating erythroid cells are the ubiquitous proteins Myc, USF1, USF2, and TFII-I, as well as the hematopoiesis-specific transcription ..
  56. ncbi Subtle distinct regulations of late erythroid molecular events by PI3K/AKT-mediated activation of Spi-1/PU.1 oncogene autoregulation loop
    O Breig
    mRNA Metabolism in Normal and Pathological Cells, CGMC, CNRS, Universite Lyon 1, Villeurbanne, France
    Oncogene 29:2807-16. 2010
    ..1 inhibits 4.1R erythroid splicing through two different pathways, and bring new insights into the extracellular signal impact triggered by erythropoietin on late erythroid regulatory program, including pre-mRNA splicing...
  57. ncbi K-CL co-transport plays an important role in normal and beta thalassemic erythropoiesis
    Lucia De Franceschi
    Department of Clinical and Experimental Medicine, University of Verona, Italy
    Haematologica 92:1319-26. 2007
    ..We evaluated the possible role of K-Cl co-transport (KCC) in both normal and beta-thalassemic erythropoiesis in vitro...
  58. pmc Erythroid/myeloid progenitors and hematopoietic stem cells originate from distinct populations of endothelial cells
    Michael J Chen
    Abramson Family Cancer Research Institute and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Cell Stem Cell 9:541-52. 2011
    ..The data indicate that EMPs and HSCs differentiate from distinct populations of hemogenic endothelial cells, with Ly6a expression specifically marking the HSC-generating hemogenic endothelium...
  59. pmc Functional conservation of erythropoietin signaling in zebrafish
    Noelle Paffett-Lugassy
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Blood 110:2718-26. 2007
    ..Together, the characterization of zebrafish epo and epor demonstrates the conservation of an ancient program that ensures proper red blood cell numbers during normal homeostasis and under hypoxic conditions...
  60. pmc Defective erythroid differentiation in miR-451 mutant mice mediated by 14-3-3zeta
    David M Patrick
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Genes Dev 24:1614-9. 2010
    ..These findings reveal an essential role of 14-3-3zeta as a mediator of the proerythroid differentiation actions of miR-451, and highlight the therapeutic potential of miR-451 inhibitors...
  61. ncbi Involvement of miRNA in erythroid differentiation
    Nicoletta Bianchi
    BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
    Epigenomics 4:51-65. 2012
    ..In this review, we present and discuss the available literature on the expression of miRNAs in erythroid cells. There are several experimental systems that can be employed for studies focusing on the relationship between ..
  62. pmc Apoptosis induced by JAK2 inhibition is mediated by Bim and enhanced by the BH3 mimetic ABT-737 in JAK2 mutant human erythroid cells
    Britta Will
    Division of Hematology Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Blood 115:2901-9. 2010
    ..These data suggest that Bim is a key effector molecule in JAK2 inhibition-induced apoptosis and that targeting this apoptotic pathway could be a novel therapeutic strategy for patients with activating JAK2 mutations...
  63. ncbi Hypoxia induces erythroid-specific 5-aminolevulinate synthase expression in human erythroid cells through transforming growth factor-beta signaling
    Kiriko Kaneko
    Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Sendai, Miyagi, Japan
    FEBS J 276:1370-82. 2009
    ..However, it has also been suggested that hypoxia could enhance hemoglobin production in erythroid cells directly...
  64. ncbi Heme-dependent up-regulation of the alpha-globin gene expression by transcriptional repressor Bach1 in erythroid cells
    Tsuyoshi Tahara
    Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606 8585, Japan
    Biochem Biophys Res Commun 324:77-85. 2004
    ..These results indicated that heme plays an important role in the induction of alpha-globin gene expression through disrupting the interaction of Bach1 and the NA site in HS-40 enhancer in erythroid cells.
  65. pmc PU.1 directly regulates cdk6 gene expression, linking the cell proliferation and differentiation programs in erythroid cells
    Kevin S Choe
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 285:3044-52. 2010
    ..PU.1 also may be involved in controlling proliferation of erythroid cells. We reported previously that the G(1) phase-specific cyclin-dependent kinase 6 (CDK6) also blocks erythroid ..
  66. pmc Erythropoietin-induced phosphorylation/degradation of BIM contributes to survival of erythroid cells
    Randolph M Abutin
    Department of Pharmacology, Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298 0035, USA
    Exp Hematol 37:151-8. 2009
    ..We investigated here the role of BIM in erythropoietin (EPO)-mediated survival in erythroid cells.
  67. pmc Ferrochelatase forms an oligomeric complex with mitoferrin-1 and Abcb10 for erythroid heme biosynthesis
    Wen Chen
    Department of Medicine, Hematology Division, Brigham and Women s Hospital, 1 Blackfan Circle, Boston, MA 02115, USA
    Blood 116:628-30. 2010
    In erythroid cells, ferrous iron is imported into the mitochondrion by mitoferrin-1 (Mfrn1). Previously, we showed that Mfrn1 interacts with Abcb10 to enhance mitochondrial iron importation...
  68. pmc Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming
    Orapan Sripichai
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 114:2299-306. 2009
    ..Thus, cytokine-mediated signal transduction in adult erythroid cells causes significant changes in the pattern of globin gene and protein expression that are associated with ..
  69. pmc Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells
    Shalini A Muralidhar
    Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas 75080, USA
    J Biol Chem 286:2343-53. 2011
    ..Moreover, enforced HDAC9 expression increased γ-globin mRNA levels by 2.5-fold with a simultaneous 7-fold increase in HbF. Collectively, these data support a positive role for HDAC9 in γ-globin gene regulation...
  70. pmc FOG1 requires NuRD to promote hematopoiesis and maintain lineage fidelity within the megakaryocytic-erythroid compartment
    Gregory D Gregory
    Division of Hematology, Children s Hospital of Philadelphia, PA 19104, USA
    Blood 115:2156-66. 2010
    ..Normal FOG1 function in late-stage erythroid cells and MK requires interaction with the chromatin remodeling complex NuRD...
  71. ncbi HSP27 controls GATA-1 protein level during erythroid cell differentiation
    Aurelie de Thonel
    INSERM U866, Faculty of Medicine and Pharmacy, University of Burgundy, 7 Boulevard Jeanne d Arc, Dijon, France
    Blood 116:85-96. 2010
    ..differentiation; that is, in the human erythroleukemia cell line, K562 induced to differentiate into erythroid cells on hemin exposure and CD34(+) human cells ex vivo driven to erythroid differentiation in liquid culture, ..
  72. pmc Identification of ZBP-89 as a novel GATA-1-associated transcription factor involved in megakaryocytic and erythroid development
    Andrew J Woo
    Department of Pediatric Oncology, Children s Hospital Boston Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 28:2675-89. 2008
    ....
  73. pmc Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus
    Yu Chiau Shyu
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China
    Mol Cell Biol 25:10365-78. 2005
    ..These data together point to a model of mammalian beta-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells.
  74. pmc Decreased differentiation of erythroid cells exacerbates ineffective erythropoiesis in beta-thalassemia
    Ilaria V Libani
    Department of Pediatric Hematology Oncology, Children s Cancer and Blood Foundation Laboratories, Weill Medical College of Cornell University, New York, NY 10021, USA
    Blood 112:875-85. 2008
    ..affected by beta-thalassemia and observed, unexpectedly, a relatively small increase in apoptosis of their erythroid cells compared with healthy mice...
  75. pmc Expression of GATA-1 in a non-hematopoietic cell line induces beta-globin locus control region chromatin structure remodeling and an erythroid pattern of gene expression
    Michael E Layon
    Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH 03756, USA
    J Mol Biol 366:737-44. 2007
    ..These results imply that GATA-1 is sufficient to direct chromatin structure reorganization within the beta-globin LCR and an erythroid pattern of gene expression in the absence of other hematopoietic transcription factors...
  76. ncbi Flow cytometry evaluation of erythroid dysplasia in patients with myelodysplastic syndrome
    M G Della Porta
    Division of Hematology, University of Pavia Medical School, IRCCS Policlinico S Matteo, Pavia, Italy
    Leukemia 20:549-55. 2006
    ..This flow-cytometry approach provides an accurate quantitative evaluation of erythroid dysplasia and allows a reliable diagnosis of sideroblastic anemia, and may therefore be a useful tool in the work-up of patients with MDS...
  77. ncbi Histone H3 lysine 9 methylation and HP1gamma are associated with transcription elongation through mammalian chromatin
    Christopher R Vakoc
    Division of Hematology, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    Mol Cell 19:381-91. 2005
    ..Both the presence of HP1gamma and H3K9 methylation are dependent upon elongation by RNA polymerase II. These findings demonstrate novel roles for H3K9 methylation and HP1gamma in transcription activation...
  78. ncbi Increase in gamma-globin mRNA content in human erythroid cells treated with angelicin analogs
    Ilaria Lampronti
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara, 74, 44100, Ferrara, Italy
    Int J Hematol 90:318-27. 2009
    ....
  79. pmc Mechanism of human Hb switching: a possible role of the kit receptor/miR 221-222 complex
    Marco Gabbianelli
    Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, 00161 Rome, Italy
    Haematologica 95:1253-60. 2010
    ..MicroRNAs (miRs) play a pivotal role in normal hematopoiesis: downmodulation of miR-221/222 stimulates human erythropoietic proliferation through upmodulation of the kit receptor...
  80. pmc Lestaurtinib (CEP701) is a JAK2 inhibitor that suppresses JAK2/STAT5 signaling and the proliferation of primary erythroid cells from patients with myeloproliferative disorders
    Elizabeth O Hexner
    Division of Hematology and Oncology, University of Pennsylvania, Philadelphia, USA
    Blood 111:5663-71. 2008
    ..1.7 cells, which are dependent on mutant JAK2 activity for growth in vitro and in xenograft models. Erythroid cells expanded from primary CD34(+) cells from patients with MPDs were inhibited by lestaurtinib at concentrations ..
  81. ncbi Stem cell transplantation demonstrates that Sox6 represses epsilon y globin expression in definitive erythropoiesis of adult mice
    Orit Cohen-Barak
    Department of Pediatrics, University of Arizona, College of Medicine, Tucson, AZ 85724, USA
    Exp Hematol 35:358-67. 2007
    ..Therefore, Sox6 may provide a novel therapeutic target by reactivating epsilon y in patients with hemoglobinopathies such as sickle cell anemia and beta-thalassemia...
  82. ncbi Activin A induction of erythroid differentiation through MKK6-p38alpha/p38beta pathway is inhibited by follistatin
    Huei Mei Huang
    Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
    J Cell Physiol 223:687-94. 2010
    ..These results demonstrate that activin A induces erythroid differentiation of K562 cells through activation of MKK6-p38alpha/p38beta pathway and follistatin inhibits those effects...
  83. ncbi Role of intergenic human gamma-delta-globin sequences in human hemoglobin switching and reactivation of fetal hemoglobin in adult erythroid cells
    Arthur Bank
    Department of Medicine, Columbia University, 701 West 168th St, Rm 1604, New York, NY 10032, USA
    Ann N Y Acad Sci 1054:48-54. 2005
    ....
  84. pmc The first trimester human placenta is a site for terminal maturation of primitive erythroid cells
    Ben Van Handel
    Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, 621 Charles E Young Drive S, Los Angeles, CA 90095, USA
    Blood 116:3321-30. 2010
    ..Extravascular ζ-globin(+) primitive erythroid cells were found in placental villi between 5-7 weeks of development, at which time the frequency of enucleated ..
  85. ncbi Erythroid induction of chronic myelogenous leukemia K562 cells following treatment with a photoproduct derived from the UV-A irradiation of 5-methoxypsoralen
    Alessia Salvador
    Department of Pharmaceutical Sciences, University of Padova, Via Marzolo, 5, 35151, Padova, Italy
    ChemMedChem 5:1506-12. 2010
    ..The major photoproduct was characterized and analyzed, and it was found to induce erythroid differentiation of K562 cells and inhibit NF-kappaB/DNA interactions...
  86. ncbi Mitochondrial uncoupling protein 2 gene transcript levels are elevated in maturating erythroid cells
    Pavel Flachs
    Department of Adipose Tissue Biology, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
    FEBS Lett 581:1093-7. 2007
    ..High UCP2 transcript levels were detected in reticulocytes and other maturating erythroid cells in peripheral blood of mice exposed to hypoxia, and in umbilical cord blood of human neonates and peripheral ..
  87. ncbi Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia
    Charalampos Pontikoglou
    Department of Hematology of the University of Crete School of Medicine, Heraklion, Crete, Greece
    Exp Hematol 34:1312-22. 2006
    ..The aim of this study is to probe the mechanisms underlying anemia in patients with chronic idiopathic neutropenia (CIN) by evaluating parameters of bone marrow (BM) erythropoiesis...
  88. ncbi Neurokinin-B transcription in erythroid cells: direct activation by the hematopoietic transcription factor GATA-1
    Saumen Pal
    University of Wisconsin Medical School, Molecular and Cellular Pharmacology Program, Department of Pharmacology, Madison, Wisconsin 53706, USA
    J Biol Chem 279:31348-56. 2004
    ..Tac-2 was expressed in normal murine erythroid cells and was induced upon ex vivo erythropoiesis...
  89. ncbi Erythroid cells in immunoregulation: characterization of a novel suppressor factor
    Galina V Seledtsova
    Department of Immunohematology, Institute of Clinical Immunology, Russian Academy of Medical Sciences, 14 Yadrintsevskaya Street, 630099 Novosibirsk, Russia
    Immunol Lett 93:171-8. 2004
    Nucleated erythroid cells (EC) have been previously reported to possess a potent natural suppressor (NS) activity for B-cell responses...
  90. pmc SOD2 deficient erythroid cells up-regulate transferrin receptor and down-regulate mitochondrial biogenesis and metabolism
    Florent M Martin
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 6:e16894. 2011
    ..SA is primarily an acquired, clonal marrow disorder occurring in individuals over 60 years of age with uncertain etiology...
  91. ncbi Evolutionary conservation of KLF transcription factors and functional conservation of human gamma-globin gene regulation in chicken
    Priyadarshi Basu
    Department of Human Genetics, Virginia Commonwealth University, P O Box 980033, 1101 E Marshall Street, Richmond, VA 23298 0033, USA
    Genomics 84:311-9. 2004
    ..This indicates that a CACCC-binding factor(s) important for gamma-globin expression functions in 5-day chicken red cells...
  92. ncbi Erythropoiesis, anemia and the bone marrow microenvironment
    Carl R Walkley
    St Vincent s Institute of Medical Research, Department of Medicine, St Vincent s Hospital, University of Melbourne, 9 Princes St, Fitzroy, VIC 3065, Australia
    Int J Hematol 93:10-3. 2011
    ....
  93. pmc Recombinant erythroid Kruppel-like factor fused to GATA1 up-regulates delta- and gamma-globin expression in erythroid cells
    Jianqiong Zhu
    Molecular and Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and
    Blood 117:3045-52. 2011
    ..Our studies demonstrate that EKLF-GATA1 fusion proteins can enhance δ-globin expression through interaction with the δ-globin promoter, and may represent a new genetic therapeutic approach to β-hemoglobinopathies...
  94. ncbi c-Jun blocks cell differentiation but not growth inhibition or apoptosis of chronic myelogenous leukemia cells induced by STI571 and by histone deacetylase inhibitors
    Huei Mei Huang
    Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan
    J Cell Physiol 218:568-74. 2009
    ..These results suggest that c-Jun may modulate anticancer drugs-induced cell differentiation but not growth inhibition and apoptosis in CML cells...
  95. ncbi Downregulation of the Spi-1/PU.1 oncogene induces the expression of TRIM10/HERF1, a key factor required for terminal erythroid cell differentiation and survival
    Rand Blaybel
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Lyon 1, Bat Gregor Mendel, 16, rue R Dubois, 69622 Villeurbanne, France
    Cell Res 18:834-45. 2008
    ..1 downregulation acts on late erythroid differentiation through at least two pathways, one of which requires TRIM10/HERF1 upregulation and parallels the Spi-1/PU.1-induced Fli-1 shutoff regulatory cascade...
  96. ncbi Induction of gamma-globin mRNA, erythroid differentiation and apoptosis in UVA-irradiated human erythroid cells in the presence of furocumarin derivatives
    Giampietro Viola
    Department of Pharmaceutical Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy
    Biochem Pharmacol 75:810-25. 2008
    ..Altogether, these data suggest that psoralen derivatives warrant further evaluation as potential therapeutic drugs in beta-thalassemia and sickle cell anemia...
  97. pmc A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells
    Claudio Cantu'
    Dipartimento di Biotecnologie e Bioscienze, Universita di Milano Bicocca, Milan, Italy
    Nucleic Acids Res 39:486-501. 2011
    The Sox6 transcription factor plays critical roles in various cell types, including erythroid cells. Sox6-deficient mice are anemic due to impaired red cell maturation and show inappropriate globin gene expression in definitive ..
  98. ncbi p210(BCR-ABL) reprograms transformed and normal human megakaryocytic progenitor cells into erythroid cells and suppresses FLI-1 transcription
    D Buet
    INSERM, U790, Institut Gustave Roussy, Villejuif, France
    Leukemia 21:917-25. 2007
    ..these results demonstrate that alteration of signal transduction via p210(BCR-ABL) reprograms MK cells into erythroid cells by a downregulation of FLI-1...
  99. pmc Embryonic and fetal beta-globin gene repression by the orphan nuclear receptors, TR2 and TR4
    Osamu Tanabe
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109 2200, USA
    EMBO J 26:2295-306. 2007
    ..Silencing of both the embryonic and fetal beta-type globin genes is delayed in definitive erythroid cells of Tr2 and Tr4 null mutant mice, whereas in transgenic mice that express dominant-negative TR4 (dnTR4), human ..
  100. ncbi Effect of iron chelators on labile iron and oxidative status of thalassaemic erythroid cells
    Eugenia Prus
    Department of Haematology, Hadassah Hebrew University Medical Centre, Jerusalem, Israel
    Acta Haematol 123:14-20. 2010
    ..We have previously shown that thalassaemic RBCs and their precursors have more LIP and ROS than their normal counterparts. We now report the effect of clinically relevant iron chelators on these parameters...
  101. ncbi Chicken alpha-globin switching depends on autonomous silencing of the embryonic pi globin gene by epigenetics mechanisms
    Héctor Rincón-Arano
    Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Apartado Postal 70 242, Mexico 04510, DF, Mexico
    J Cell Biochem 108:675-87. 2009
    ..We propose that epigenetic mechanisms are necessary for in vivo chicken alpha-globin gene switching through differential gene silencing of the embryonic alpha-globin gene in order to allow proper activation of adult alpha-globin genes...

Research Grants65

  1. Mechanisms of hepcidin effects in the anemia of chronic disease
    Robert T Means; Fiscal Year: 2013
    ..effects of Epo and inflammatory cytokines on FPN variant expression in erythroid cells. 2. Determination of the interactions of hepcidin and cytokines in bone marrow macrophage iron transport...
  2. Molecular Biology of Human Erythrocyte Alpha Spectrin
    Patrick G Gallagher; Fiscal Year: 2013
    ..function, and regulation and allow us to identify a common regulatory signature that controls expression in erythroid cells. The studies in this aim combine high throughput genomic technologies with functional studies of gene ..
  3. Erythropoietin in Non-Erythroid Cells: Function and Regulation
    Josef T Prchal; Fiscal Year: 2013
    ..The possibility that Epo may play a beneficial as well as detrimental role in endothelial/cardiovascular, neurological abnormalities, and cancers is relevant to a significant portion of VA patients. ..
  4. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2013
    ..chicken 2-globin insulator HS4, which maintains an accessible chromatin conformation over the globin genes in erythroid cells. Our preliminary data demonstrate that USF1 interacts with large co-activator complexes containing two ..
  5. PlGF-HIF1a-miRNA Axis in Sickle Pulmonary Hypertension
    Punam Malik; Fiscal Year: 2013
    ..have shown that placenta growth factor (PlGF), an angiogenic growth factor produced in high amounts by sickle erythroid cells, induces expression of the potent pulmonary vasoconstrictor ET-1, and a procoagulant, plasminogen activator ..
  6. Understand and Improve Iron Distribution and Erythropoiesis in Beta-Thalassemia
    Yelena Ginzburg; Fiscal Year: 2013
    ..my hypothesis that exogenous transferrin restricts iron entry into erythroid precursors and protects early erythroid cells from heme toxicity, will provide insight into the physiology responsible for the phenotype in beta- ..
  7. SIGNAL TRANSDUCTION MECHANISMS OF ERYTHROPOIETIN
    Barbara A Miller; Fiscal Year: 2011
    ..TRPC3 and TRPC6 are members of the TRPC subfamily which are expressed in human erythroid cells. TRPC3 is activated by Epo, while TRPC6 inhibits TRPC3 activation...
  8. AKT Signaling and Oxidative Stress Regulation of Erythropoiesis
    Saghi Ghaffari; Fiscal Year: 2013
    A tight coordination of erythroid cell division and maturation is required for daily production of erythroid cells and for immediate response to clinical conditions in which stress erythropoiesis is in demand...
  9. Reactivation of Fetal Gamma-globin Genes for the Treatment of Beta-globin Disorde
    Osamu Tanabe; Fiscal Year: 2010
    ..Both primary mouse and human erythroid cells differentiated ex vivo from adult hematopoietic progenitors will be explored as possible model systems to ..
  10. Globin Gene Expression during Erythroid Differentiation
    Gordon D Ginder; Fiscal Year: 2010
    ..of DMA methylation in the developmental regulation of embryonic and fetal globin gene expression in adult erythroid cells. The long-term goal of this project is to characterize mechanistic components of developmental globin gene ..
  11. Nonsense Mediated RNA Decay Regulation in Erythropoiesis &Thalassemia
    LAWRENCE BENJAMIN GARDNER; Fiscal Year: 2012
    ..But because NMD has not been thought of as a regulated pathway, its activity in normal or thalassemic erythroid cells has not been closely studied, and its significance in erythropoiesis has not been determined...
  12. Identification of a Molecular Signature for Barrier Insulators
    LAURIE ANN STEINER; Fiscal Year: 2013
    ..barrier insulators will be subjected to quantitative ChIP analyses and PEV assays in both erythroid and non-erythroid cells, and selected sites will be studied in detail in transgenic mouse models...
  13. A Comparative Approach to Globin Regulation/Thalassemia
    LEONARD IRA ZON; Fiscal Year: 2012
    ..bringing the zebrafish LCR into apposition with globin genes drives high level of expression within erythroid cells. Transgenic fish are being created with human globin BACs to evaluate conservation of regulatory elements...
  14. Genetic and Epigenetic Regulation of Hematopoiesis.
    Shireen Saleque; Fiscal Year: 2013
    ..of the erythroid and megakaryocytic lineages and for proper differentiation of primitive (yolk sac derived) erythroid cells. Consistent with the decisive roles of these genes in normal hematopoiesis, they exhibit malignant properties ..
  15. Novel Immunodeficiency caused by TFRC Mutation
    RAIF SALIM GEHA; Fiscal Year: 2013
    ..A potential candidate is the ferrireductase Steap3, which is selectively expressed in erythroid cells, co-localizes with TfR1, and has a 288YQRF291 internalization signal motif...
  16. Zebrafish Genetics to Identify Genes Involved in Hematopoiesis
    DHVANIT INDRAVADAN SHAH; Fiscal Year: 2013
    ..In contrast, sav shows anemia due to a lack of erythroid cells. sav lacks expression markers for HSPC (scl/tal1), myeloerythroid progenitor (gata-1), erythroid ((-globin) ..
  17. Transactivation of Fetal Hemoglobin
    Kenneth R Peterson; Fiscal Year: 2011
    ..enforced expression (gain-of-function) or knockdown of expression (loss-of function) of TSPYL1 and FGIF in erythroid cells to ascertain phenotypic or developmental effects in vivo...
  18. Structure and Function of the Human Beta-Globin Locus Control Region
    JORG BUNGERT; Fiscal Year: 2013
    ..will be addressed in transgenic mice by examining if the LCR is sufficient to form transcription foci in erythroid cells and how transcription factor upstream stimulatory factor (USF) in conjunction with erythroid specific ..
  19. GATA1 Mutation in Defective Erythropoiesis
    John D Crispino; Fiscal Year: 2013
    ..provided by applicant): GATA1 is a transcription factor that is required for survival and maturation of erythroid cells. In the absence of GATA1, mouse embryos die from anemia in mid-gestation...
  20. DEVELOPMENTAL BIOLOGY OF HUMAN ERYTHROPOIESIS
    Stuart H Orkin; Fiscal Year: 2013
    ..Projects in this Program Project grant addresses important aspects of the expression program and function of erythroid cells Findings from these studies will provide new opportunities for the design of novel approaches to the ..
  21. Extending GWAS at the BCL11A locus to novel therapeutics for HbF induction
    Stuart H Orkin; Fiscal Year: 2010
    ..Specifically;the gene expression profile of adult erythroid cells in which BCL11A expression is knocked-down will be determined and compared with the genomic chromatin ..
  22. Global Predictions and Tests of Erythroid Regulation
    Yu Zhang; Fiscal Year: 2013
    ..biochemical features associated with regulation to reach a more complete understanding of gene regulation in erythroid cells. Specifically, we propose to use high throughput biochemical assays such as chromatin immunoprecipitation ..
  23. Hematological consequences of FLVCR, a cytoplasmic heme export protein
    Janis L Abkowitz; Fiscal Year: 2013
    ..This grant will test our hypothesis by directly measuring free heme in developing erythroid cells with a luciferase reporter containing the MARE sequences (Bach1 binding site) of the [unreadable]-globin ..
  24. Opposing and complementary roles of KLF1 and KLF2 in erythropoiesis
    Joyce A Lloyd; Fiscal Year: 2012
    ..and KLF2 in organizing the global chromatin structure of the [unreadable]-globin locus in embryonic and fetal erythroid cells. KD in in vitro differentiated CD34+ human cord blood cells and knockout (KO) mouse models will be used to ..
  25. Barrier Insulators in Erythropoiesis
    Patrick G Gallagher; Fiscal Year: 2013
    ..aim one is to identify a common regulatory signature associated with functional barrier insulators in human erythroid cells. These studies address the hypothesis that there is a common regulatory signature for cell-type specific ..
  26. Regulation of autophagy by AMPK, Hsp90-Cdc37 and Ulk1 in erythroid cells
    Mondira Kundu; Fiscal Year: 2013
    ....
  27. Functional proteomics in differentiating erythroid cells
    IOANNIS JOHN STROUBOULIS; Fiscal Year: 2013
    ..a way that associations of the ?-globin genes are favored over that of the adult ?-globin gene in definitive erythroid cells. We propose to perform a comprehensive analysis of the composition and function of protein complexes ..
  28. The role of Dock4 in normal and aberrant erythropoiesis
    Amittha Wickrema; Fiscal Year: 2013
    ..Aim 3 we will determine involvement of DOCK4 in the pathogenesis of anemia associated with MDS using primary erythroid cells isolated from MDS patients...
  29. MicroRNAs and hematopoietic differentiation
    Harvey F Lodish; Fiscal Year: 2013
    ..We showed that Myb is one conserved mRNA target of miR-15a and 16-1 in erythroid cells that regulate 3 globin expression...
  30. Epigenetic Control of Hematopoietic Gene Expression
    Gerd A Blobel; Fiscal Year: 2013
    ..Using a mouse model in which the FOG1-NuRD interaction is disrupted, we observed that in developing erythroid cells and megakaryocytes NuRD is required for both repression and activation of specific genes...
  31. HRI-eIF2a Phosphorylation Signaling in Oxidative Stress and Erythropoiesis
    Jane Jane Chen; Fiscal Year: 2012
    ..mechanism of ineffective erythropoiesis and the functions of HRI in the growth and differentiation of erythroid cells under stress conditions and in disease states...
  32. Effects of developmental stage and of KLF2 on mouse erythroid genetic profiles
    Joyce A Lloyd; Fiscal Year: 2011
    An important unresolved question is how the programs to generate primitive (embryonic) and definitive (adult) erythroid cells differ...
  33. Novel Determinants of Terminal Erythroid Maturation
    Saghi Ghaffari; Fiscal Year: 2013
    ..FoxO3 and mTOR were known to have critical independent functions to control autophagy in non-erythroid cells. Autophagy mediates the consumption of damaged cellular components and differentiation-associated cellular ..
  34. 5-Hydroxymethylcytosine and Globin Gene Switching
    Donald Lavelle; Fiscal Year: 2013
    ..cells that is catalyzed by the TET dioxygenase family, are associated with the gamma-globin promoter in erythroid cells expressing elevated levels of gamma-globin...
  35. Regulation of Erythroid Terminal Differentiation by Long Noncoding RNAs
    Wenqian Hu; Fiscal Year: 2013
    ..The differentiation and maturation of erythroid cells are carefully controlled at multiple steps to ensure the proper generation of red blood cells under various ..
  36. Function of human &mouse Beta-globin locus control regions
    Mark T Groudine; Fiscal Year: 2013
    ..histone modifications and sequence interactions, will be determined by DSL analyses in stage-specific erythroid cells from mutant mice and in cell lines containing WT and modified h11s passed through different cellular ..
  37. Mechanisms of Impaired Erythropoiesis in Post Burn Anemia of Critical Illness
    KUZHALI MUTHUMALAIAPPAN; Fiscal Year: 2013
    ..erythroid progenitors and that beta-adrenergic blockade following burn significantly improves the bone marrow erythroid cells. We have also established that burn injury offsets the balance between erythroid specific and monocyte ..
  38. Erythroid heme synthesis regulation via the ferrochelatase [2Fe-2S] cluster
    Harry A Dailey; Fiscal Year: 2013
    ..3) if th putative regulatory role of the cluster is unique to ferrochelatases of organisms that possess erythroid cells, and 4) if the [2Fe-2S] cluster exerts its function via an intra-protein action tha directly impacts ..
  39. PROGRAM PROJECT: RED CELL MEMBRANE STUDIES
    MOHANDAS NARLA; Fiscal Year: 2012
    ..of the molecular and structural basis for the functions of an important family of cytoskeletal proteins in erythroid cells;2) Obtain detailed mechanistic understanding of the functional and biological consequences of interaction ..
  40. MOLECULAR ANALYSIS OF NORMAL AND THALASSEMIC DNA
    Stuart H Orkin; Fiscal Year: 2013
    ..The aims of this project address the mechanisms by which BCL11A acts in erythroid cells. Aim 1 addresses the structure and potential function of a unique isoform of BCL11A that is present in human ..
  41. Metalloreductases in Medicine
    Mark D Fleming; Fiscal Year: 2010
    ..For example, erythroid cells are uniquely dependent upon the transferrin cycle for iron acquisition...
  42. Genetic Modifiers of Beta-like Globin Gene Switching
    Luanne L Peters; Fiscal Year: 2013
    ..WT-EKLF DNA targets, and differences in gene expression in Nan vs. WT erythroid cells will converge to identify novel regulators of [unreadable]- like globin switching, thereby providing ..
  43. Whole Genome Chromatin Interaction Analysis using Pair-End-diTagging (CIA-PET)
    Yijun Ruan; Fiscal Year: 2010
    ..in 3 biological systems: a) Chromatin interactions between hemoglobin genes and regulatory elements in mouse erythroid cells. b) Tertiary networks of transcription regulation mediated by Nanog and Oct4 in mouse embryonic stem cells...
  44. Gene therapy for Cooley's anemia in a new mouse model
    Stefano Rivella; Fiscal Year: 2010
    ..protein (hrGFP), in all the hematopoietic lineages and expression of the human beta-globin gene in erythroid cells (TNS9+GFP)...
  45. Globin Gene Expression in Sickle Cell Genotype-Specific iPS cells
    Martin H Steinberg; Fiscal Year: 2013
    ..search for novel HbF genetic modifiers associated with HbF levels by examining gene expression in iPS derived erythroid cells;5) determine which novel therapeutics discovered in high throughput screens can enhance and maintain high ..
  46. NLRP1 activation induces deletion of hematopoietic progenitor cells
    BEN ADAM CROKER; Fiscal Year: 2013
    ..virus (LCMV) infection or chemotherapy, Nlrp1aQ593P/Q593P mice fail to repopulate myeloid, lymphoid and erythroid cells. Conversely, NLRP1-deficient mice show enhanced recovery from LCMV infection and chemotherapy...
  47. Feline Leukemia Virus C Receptor Heme Export and Erythroid Differentiation
    SIOBAN BRIDGET KEEL; Fiscal Year: 2010
    ..1) to determine if intracellular free heme increases when FLVCR expression or function is downregulated in erythroid cells;2) to determine if erythroid differentiation is blocked or apoptosis is induced when FLVCR function or ..
  48. Molecular Regulation of Erythroid Differentiation
    Min Chen; Fiscal Year: 2013
    ..A Bcl-2 family member, Nix/Bnip3L, has been found to regulate mitochondrial clearance in differentiating erythroid cells. In Nix-deficient red blood cells, the formation of autophagosomes proceeded normally...
  49. Genetic Therapy for CNS Manifestations in MPS I via BBB-targeted Protein Delivery
    Dao Pan; Fiscal Year: 2012
    ..distribution profile will be studied in the CNS and peripheral organs, targeting the liver or HSC-derived erythroid cells as depot organ for tissue-specific transgene production in mice with different ages (Specific Aim 2)...
  50. REGULATION AND FUNCTION OF A HUMAN EMBRYONIC GLOBIN
    J Eric Russell; Fiscal Year: 2012
    ..of embryonic ?-globin mRNA dictates its stability and translation when expressed in adult-stage, definitive erythroid cells. The Aims utilize techniques that capitalize on the experience of the applicant laboratory, as well as ..
  51. Epigenetic regulation of BCL11A in the hemoglobin switch
    DANIEL EVAN BAUER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): A key developmental transition occurs in erythroid cells around the time of birth characterized by the switch from fetal to adult hemoglobin (from 1232 to 1222)...
  52. Role of KLF2 in erythropoiesis and globin expression
    Joyce A Lloyd; Fiscal Year: 2010
    ..The stage of maturation of KLF2-/- embryonic erythroid cells will be determined with a flow cytometry assay, and the ability of these cells to differentiate in vitro with ..
  53. Long-range function of the ERV-9 LTR in regulating globin gene switching
    DOROTHY Y TUAN; Fiscal Year: 2012
    ..unreadable]-globin gene and re-activated the [unreadable]-globin gene during erythroid development in the erythroid cells of the Tg mice...
  54. Inactivation of LSD1 as a possible treatment for sickle cell disease
    James Douglas Engel; Fiscal Year: 2013
    ..We found that in human CD34+ hematopoietic stem cells induced to differentiate into erythroid cells in vitro, HbF was induced to therapeutic levels in a TC dosage-dependent manner...
  55. Next Generation Therapeutics for Hemoglobinopathies
    SUSAN PARK PERRINE; Fiscal Year: 2012
    ..unrecognized, highly potent, HbF-inducing drugs in reporter gene assays and confirmed their activity in erythroid cells cultured from normal subjects...
  56. Selective HDAC1/2 inhibitors for hemoglobinopathies
    Matthew B Jarpe; Fiscal Year: 2013
    ..that are selective for HDAC1 and 2 versus other HDACs can induce the expression of fetal globin in adult erythroid cells. We have generated a set of selective HDAC1/2 inhibitors with drug like properties...
  57. Functions, mechanisms, and therapeutic potential of chromatin looping
    Gerd A Blobel; Fiscal Year: 2013
    ..Specifically, we showed that tethering Ldb1 to the locus via artificial zinc finger proteins in immature erythroid cells is sufficient to promote a looped interaction between the [unreadable]-globin enhancer and promoter and ..
  58. Erythroid stage-specific transcriptome expression, dynamics, and regulation
    LIOR S PACHTER; Fiscal Year: 2013
    ..Aim 1 will define the mouse erythroid stage-specific transcriptome using highly purified FACS-sorted erythroid cells from bone marrow (proerythroblasts as well as basophilic, polychromatic, and orthochromatic erythroblast ..
  59. NOVEL GLOBIN GENE MODULATORS
    SUSAN PARK PERRINE; Fiscal Year: 2013
    ..We will apply these 2 experiences to determine 1) which novel therapeutics most effectively augment HbF in erythroid cells cultured from beta thalassemia patients, genotyped for 2 major subtypes of thalassemia, or patients with ..
  60. Nonsense codon activation of endonuclease-mediated mRNA decay
    Daniel R Schoenberg; Fiscal Year: 2012
    Project summary/abstract In erythroid cells a premature termination codon (PTC) in exons 1 or 2 of the beta-globin gene activates a cytoplasmic endonuclease that degrades beta-globin mRNA...
  61. Beta 1 integrins in erythropoiesis
    THALIA STAMATOYANNOPOULOS; Fiscal Year: 2013
    ..How the information from other important players in Erythropoiesis is intercepted through integrins on erythroid cells for addressing stress responses is unclear and will be addressed in SA2...
  62. Red Cell Band 4.1 - Developmental Changes in RNA Splicing
    John G Conboy; Fiscal Year: 2010
    This renewal proposal explores mechanisms that regulate alternative pre-mRNA splicing in differentiating erythroid cells, focusing on cis-regulatory sequences and trans-acting splicing factor proteins that regulate erythroid stage-..
  63. FETAL ERYTHROID CELLS IN MATERNAL CIRCULATION
    STEPHEN GRANT; Fiscal Year: 1999
    ..molecular genetics make it feasible to consider genetic testing of the fetus via the small numbers of fetal erythroid cells that migrate across the placenta into the maternal circulation during pregnancy...
  64. IN VIVO SIGNALING OF THE ERYTHROPOIETIN RECEPTOR
    Saghi Ghaffari; Fiscal Year: 2002
    ..Dysfunction of EPO-R signaling results in malignancies and other disorders of erythroid cells such as erythroleukemias and polycythemia vera...
  65. PROTEOLYSIS IN HUMAN ERYTHROID CELLS
    JOSEPH SHAEFFER; Fiscal Year: 1991
    ..Cultured erythroid cells will be used to compare the properties of proteolysis of the small pool of Alpha chains in normal cells with ..