paneth cells

Summary

Summary: Differentiated epithelial cells of the INTESTINAL MUCOSA, found in the basal part of the intestinal crypts of Lieberkuhn. Paneth cells secrete GROWTH FACTORS, digestive enzymes such as LYSOZYME and antimicrobial peptides such as cryptdins (ALPHA-DEFENSINS) into the crypt lumen.

Top Publications

  1. pmc Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
    Toshiro Sato
    Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nature 469:415-8. 2011
  2. ncbi Redundant sources of Wnt regulate intestinal stem cells and promote formation of Paneth cells
    Henner F Farin
    Hubrecht Institute for Developmental Biology and Stem Cell Research and University Medical Centre Utrecht, Utrecht, The Netherlands
    Gastroenterology 143:1518-1529.e7. 2012
  3. pmc XBP1 links ER stress to intestinal inflammation and confers genetic risk for human inflammatory bowel disease
    Arthur Kaser
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Cell 134:743-56. 2008
  4. pmc A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells
    Ken Cadwell
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 456:259-63. 2008
  5. pmc Virus-plus-susceptibility gene interaction determines Crohn's disease gene Atg16L1 phenotypes in intestine
    Ken Cadwell
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell 141:1135-45. 2010
  6. ncbi Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB
    Eduard Batlle
    Department of Immunology and Center for Biomedical Genetics, University Medical Center, Utrecht, The Netherlands
    Cell 111:251-63. 2002
  7. ncbi Paneth cells, antimicrobial peptides and maintenance of intestinal homeostasis
    Charles L Bevins
    Department of Microbiology and Immunology, University of California, Davis School of Medicine, Davis, California 95616, USA
    Nat Rev Microbiol 9:356-68. 2011
  8. ncbi Paneth cells: maestros of the small intestinal crypts
    Hans C Clevers
    Hubrecht Institute KNAW, University Medical Center Utrecht, Uppsalalaan, Utrecht 3584CT, The Netherlands
    Annu Rev Physiol 75:289-311. 2013
  9. pmc Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells
    Kelli L VanDussen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI 48109, USA
    Development 139:488-97. 2012
  10. pmc Paneth cells as a site of origin for intestinal inflammation
    Timon E Adolph
    1 Division of Gastroenterology and Hepatology, Department of Medicine, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0QQ, UK 2
    Nature 503:272-6. 2013

Detail Information

Publications239 found, 100 shown here

  1. pmc Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
    Toshiro Sato
    Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nature 469:415-8. 2011
    ..Lgr5 stem cells are interspersed between terminally differentiated Paneth cells that are known to produce bactericidal products such as lysozyme and cryptdins/defensins...
  2. ncbi Redundant sources of Wnt regulate intestinal stem cells and promote formation of Paneth cells
    Henner F Farin
    Hubrecht Institute for Developmental Biology and Stem Cell Research and University Medical Centre Utrecht, Utrecht, The Netherlands
    Gastroenterology 143:1518-1529.e7. 2012
    Wnt signaling regulates multiple aspects of intestinal physiology, including stem cell maintenance. Paneth cells support stem cells by secreting Wnt, but little is known about the exact sources and primary functions of individual Wnt ..
  3. pmc XBP1 links ER stress to intestinal inflammation and confers genetic risk for human inflammatory bowel disease
    Arthur Kaser
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Cell 134:743-56. 2008
    ..Hence, intestinal inflammation can originate solely from XBP1 abnormalities in IECs, thus linking cell-specific ER stress to the induction of organ-specific inflammation...
  4. pmc A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells
    Ken Cadwell
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Nature 456:259-63. 2008
    ..ATG16L1- and ATG5-deficient Paneth cells exhibited notable abnormalities in the granule exocytosis pathway...
  5. pmc Virus-plus-susceptibility gene interaction determines Crohn's disease gene Atg16L1 phenotypes in intestine
    Ken Cadwell
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell 141:1135-45. 2010
    ..gene interaction generated abnormalities in granule packaging and unique patterns of gene expression in Paneth cells. Further, the response to injury induced by the toxic substance dextran sodium sulfate was fundamentally ..
  6. ncbi Beta-catenin and TCF mediate cell positioning in the intestinal epithelium by controlling the expression of EphB/ephrinB
    Eduard Batlle
    Department of Immunology and Center for Biomedical Genetics, University Medical Center, Utrecht, The Netherlands
    Cell 111:251-63. 2002
    ..Absorptive, enteroendocrine, and goblet cells migrate toward the villus while Paneth cells occupy the bottom of the crypts...
  7. ncbi Paneth cells, antimicrobial peptides and maintenance of intestinal homeostasis
    Charles L Bevins
    Department of Microbiology and Immunology, University of California, Davis School of Medicine, Davis, California 95616, USA
    Nat Rev Microbiol 9:356-68. 2011
    ..b>Paneth cells (specialized cells in the epithelium of the small intestine) are an important source of antimicrobial peptides ..
  8. ncbi Paneth cells: maestros of the small intestinal crypts
    Hans C Clevers
    Hubrecht Institute KNAW, University Medical Center Utrecht, Uppsalalaan, Utrecht 3584CT, The Netherlands
    Annu Rev Physiol 75:289-311. 2013
    b>Paneth cells are highly specialized epithelial cells of the small intestine, where they coordinate many physiological functions...
  9. pmc Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells
    Kelli L VanDussen
    Department of Molecular and Integrative Physiology, The University of Michigan, Ann Arbor, MI 48109, USA
    Development 139:488-97. 2012
    ..Our findings suggest that Notch targets distinct progenitor cell populations to maintain adult intestinal stem cells and to regulate cell fate choice to control epithelial cell homeostasis...
  10. pmc Paneth cells as a site of origin for intestinal inflammation
    Timon E Adolph
    1 Division of Gastroenterology and Hepatology, Department of Medicine, Addenbrooke s Hospital, University of Cambridge, Cambridge CB2 0QQ, UK 2
    Nature 503:272-6. 2013
    ..feature of inflammatory bowel disease epithelium, and several genetic risk factors of Crohn's disease affect Paneth cells. Here we show that impairment in either UPR (Xbp1(ΔIEC)) or autophagy function (Atg16l1(ΔIEC) or Atg7(ΔIEC)) ..
  11. ncbi Wnt signalling induces maturation of Paneth cells in intestinal crypts
    Johan H van Es
    Hubrecht Institute, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    Nat Cell Biol 7:381-6. 2005
    ..Whereas all other differentiated epithelial cells migrate from the crypt onto the villus, Paneth cells home towards the source of Wnt signals--that is, the crypt bottom...
  12. ncbi Paneth cells, defensins, and the commensal microbiota: a hypothesis on intimate interplay at the intestinal mucosa
    Nita H Salzman
    Department of Pediatrics, Division of Gastroenterology, The Medical College of Wisconsin, 8701 Watertown Plank Rd Milwaukee, WI 53226, USA
    Semin Immunol 19:70-83. 2007
    ..In the small intestine, Paneth cells (specialized secretory epithelial cells) produce high quantities of defensins and several other antibiotic ..
  13. ncbi Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria
    T Ayabe
    Department of Pathology, College of Medicine, University of California, Irvine, CA 92697, USA
    Nat Immunol 1:113-8. 2000
    b>Paneth cells in mouse small intestinal crypts secrete granules rich in microbicidal peptides when exposed to bacteria or bacterial antigens...
  14. pmc Paneth cells in intestinal homeostasis and tissue injury
    Sabrina Roth
    Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands
    PLoS ONE 7:e38965. 2012
    ..While during homeostasis, LR/Paneth cells appear to play a supportive role for Lgr5(+) stem cells as previously shown, upon tissue injury they switch to ..
  15. ncbi The ets-domain transcription factor Spdef promotes maturation of goblet and paneth cells in the intestinal epithelium
    Alex Gregorieff
    Hubrecht Institute, Utrecht, The Netherlands
    Gastroenterology 137:1333-45.e1-3. 2009
    ..In this study, we investigated the role of the Wnt-responsive, Ets-domain transcription factor Spdef in the differentiation of goblet and Paneth cells.
  16. pmc Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium
    Pauline Bastide
    INSERM U661, Department of Cellular and Molecular Oncology, Centre National de la Recherche Scientifique UMR5203, Université de Montpellier I and Service d Anatomie Pathologie, Centre Hospitalier Universitaire Caremeau, Nimes, France
    J Cell Biol 178:635-48. 2007
    ..factor Sox9 is expressed in the intestinal epithelium, specifically, in stem/progenitor cells and in Paneth cells. Sox9 expression requires an active beta-catenin-Tcf complex, the transcriptional effector of the Wnt pathway...
  17. ncbi The multifaceted Paneth cell
    E M Porter
    Department of Biology and Microbiology, California State University Los Angeles, 90032, USA
    Cell Mol Life Sci 59:156-70. 2002
    b>Paneth cells (PCs) were described over a century ago as granulated cells located at the base of small intestinal crypts, the 'crypts of Lieberkühn...
  18. ncbi Paneth's disease
    Jan Wehkamp
    Robert Bosch Krankenhaus Stuttgart, Auerbachstr 110, 7076 Stuttgart, Germany
    J Crohns Colitis 4:523-31. 2010
    ..A characteristic feature of small intestinal host defense is the presence of Paneth cells at the bottom of the crypts of Lieberkühn...
  19. ncbi Intestinal stem cells protect their genome by selective segregation of template DNA strands
    Christopher S Potten
    Epithelial Biology Department, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M9 4BX, UK
    J Cell Sci 115:2381-8. 2002
    ..The presence of these protective mechanisms ensures that the small intestine rarely develops cancer and that stem cells can sustain the extensive cell proliferation needed during life...
  20. pmc Symbiotic bacteria direct expression of an intestinal bactericidal lectin
    Heather L Cash
    Center for Immunology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Science 313:1126-30. 2006
    ..We propose that these proteins represent an evolutionarily primitive form of lectin-mediated innate immunity, and that they reveal intestinal strategies for maintaining symbiotic host-microbial relationships...
  21. pmc Functional intestinal stem cells after Paneth cell ablation induced by the loss of transcription factor Math1 (Atoh1)
    Aurélie Durand
    Institut Cochin, Department of Endocrinology, Metabolism and Cancer, Universite Paris Descartes, Centre National de la Recherche Scientifique Unite Mixte de Recherche 8104, 75014 Paris, France
    Proc Natl Acad Sci U S A 109:8965-70. 2012
    ..an essential intrinsic signal for the maintenance and proliferation of CBC, and it was recently proposed that Paneth cells provide a crucial niche by secreting Wingless/Int (Wnt) ligands...
  22. pmc Paneth cell marker expression in intestinal villi and colon crypts characterizes dietary induced risk for mouse sporadic intestinal cancer
    Donghai Wang
    Department of Medicine, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10467, USA
    Proc Natl Acad Sci U S A 108:10272-7. 2011
    ..This is fundamental for understanding how common sporadic tumors arise and in evaluating relative risk in the population...
  23. pmc Intact function of Lgr5 receptor-expressing intestinal stem cells in the absence of Paneth cells
    Tae Hee Kim
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:3932-7. 2012
    ..Lgr5-expressing crypt base columnar cells (CBCs) reside deep in the crypt, mingled among mature Paneth cells that are well positioned for short-range signaling...
  24. ncbi Paneth cells and innate mucosal immunity
    Andre J Ouellette
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089 9601, USA
    Curr Opin Gastroenterol 26:547-53. 2010
    ..Recent evidence shows that disruption of Paneth cell homeostasis by induction of endoplasmic reticulum stress or autophagy, with consequent apoptosis, contributes to inflammation and morbidity in a variety of experimental mouse models...
  25. ncbi Cellular inheritance of a Cre-activated reporter gene to determine Paneth cell longevity in the murine small intestine
    Heather Ireland
    Cancer Research UK Department of Oncology, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Cambridge, United Kingdom
    Dev Dyn 233:1332-6. 2005
    ..in their ability to express Cre from a somatically inducible transgene to determine the longevity of intestinal Paneth cells. In the Ahcre transgenic line induction of Cre recombinase allows constitutive activation of a Cre-activated ..
  26. ncbi The Intestinal Wnt/TCF Signature
    Laurens G van der Flier
    Hubrecht Institute, Netherlands Institute for Developmental Biology and Centre for Biomedical Genetics, Utrecht, The Netherlands
    Gastroenterology 132:628-32. 2007
    ..In colorectal cancer, activating mutations in the Wnt pathway transform epithelial cells through the inappropriate expression of a TCF4 target gene program, which is physiologically expressed in intestinal crypts...
  27. ncbi Reduced alpha-defensin expression is associated with inflammation and not NOD2 mutation status in ileal Crohn's disease
    L A Simms
    Inflammatory Bowel Disease Laboratory, Royal Brisbane and Women s Hospital Foundation, Brisbane, Australia
    Gut 57:903-10. 2008
    ..The aim of this study was to independently assess whether NOD2, alpha-defensins and Crohn's disease are linked...
  28. pmc Paneth cells directly sense gut commensals and maintain homeostasis at the intestinal host-microbial interface
    Shipra Vaishnava
    The Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 105:20858-63. 2008
    ..To address this question we studied Paneth cells, a key small intestinal epithelial lineage...
  29. pmc Reduced Paneth cell alpha-defensins in ileal Crohn's disease
    Jan Wehkamp
    Department of Microbiology and Immunology, School of Medicine, University of California, Davis, 95616, USA
    Proc Natl Acad Sci U S A 102:18129-34. 2005
    ..b>Paneth cells (PC) are the major source of antimicrobial peptides in the small intestine, including human alpha-defensins HD5 ..
  30. pmc Paneth cells and necrotizing enterocolitis: a novel hypothesis for disease pathogenesis
    Steven J McElroy
    Division of Neonatology, Department of Pediatrics, University of Iowa, Iowa City, Iowa, USA
    Neonatology 103:10-20. 2013
    ..We suggest an alternate hypothesis wherein NEC is caused by injury to Paneth cells (PCs) in the intestinal crypts...
  31. ncbi Protection against enteric salmonellosis in transgenic mice expressing a human intestinal defensin
    Nita H Salzman
    Department of Pediatrics, Division of Gastroenterology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Nature 422:522-6. 2003
    ..b>Paneth cells, specialized epithelia of the small intestinal crypt, secrete abundant alpha-defensins and other antimicrobial ..
  32. pmc NOD2 (CARD15) mutations in Crohn's disease are associated with diminished mucosal alpha-defensin expression
    J Wehkamp
    Department of Internal Medicine I, Robert Bosch Hospital, Stuttgart, Germany
    Gut 53:1658-64. 2004
    ..Human alpha defensins (HD-5 and HD-6) are antibiotic effector molecules predominantly expressed in Paneth cells of the ileum. Paneth cells also express NOD2...
  33. pmc Mouse background strain profoundly influences Paneth cell function and intestinal microbial composition
    Ajay S Gulati
    Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, North Carolina, United States of America
    PLoS ONE 7:e32403. 2012
    Increasing evidence supports the central role of Paneth cells in maintaining intestinal host-microbial homeostasis. However, the direct impact of host genotype on Paneth cell function remains unclear...
  34. ncbi CRS-peptides: unique defense peptides of mouse Paneth cells
    M L Andersson
    Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
    Mucosal Immunol 5:367-76. 2012
    ..The first part presents the current knowledge of the CRS-peptide family's biochemical characteristics and nomenclature, and the second part is devoted to the possible role of this family in the homeostasis of the gut...
  35. ncbi The Paneth cell alpha-defensin deficiency of ileal Crohn's disease is linked to Wnt/Tcf-4
    Jan Wehkamp
    Dr Margarete Fischer Bosch Institute of Clinical Pharmacology and University of Tübingen, Auerbachstrasse 12, Stuttgart, Germany
    J Immunol 179:3109-18. 2007
    ..This new link established between a human inflammatory bowel disease and the Wnt pathway/Tcf-4 provides a novel mechanism for pathogenesis in patients with ileal CD...
  36. ncbi Identification of GM-CSF in Paneth cells using single-cell RT-PCR
    Hiroaki Fukuzawa
    Department of Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawara cho, Sakyo ku, 606 8507, Kyoto, Japan
    Biochem Biophys Res Commun 312:897-902. 2003
    b>Paneth cells, granule-containing cells located at the bottom of the intestinal crypts, have a role in innate mucosal immunity...
  37. ncbi Up-regulation of autophagy in small intestine Paneth cells in response to total-body gamma-irradiation
    Nikolai V Gorbunov
    Armed Forces Radiobiology Research Institute, Bethesda, MD 20889 5603, USA
    J Pathol 219:242-52. 2009
    ..We have demonstrated upregulation of this pathway in the innate host defense Paneth cells of the small intestine (SI) due to ionizing irradiation and correlation of this effect with induction of pro-..
  38. pmc Paneth cell ablation in the presence of Klebsiella pneumoniae induces necrotizing enterocolitis (NEC)-like injury in the small intestine of immature mice
    Chunxian Zhang
    Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Dis Model Mech 5:522-32. 2012
    ..Normally, Paneth cells appear in the intestinal crypts during the first trimester of human pregnancy...
  39. pmc Tumor necrosis factor receptor 1-dependent depletion of mucus in immature small intestine: a potential role in neonatal necrotizing enterocolitis
    Steven J McElroy
    Dept of Pediatrics, Div of Neonatology, Nashville, TN 37232 0696, USA
    Am J Physiol Gastrointest Liver Physiol 301:G656-66. 2011
    ..intestinal goblet cells present in infants with NEC and found they had significantly fewer goblet cells and Paneth cells compared with controls...
  40. pmc Strain-specific polymorphisms in Paneth cell α-defensins of C57BL/6 mice and evidence of vestigial myeloid α-defensin pseudogenes
    Michael T Shanahan
    Department of Pathology and Laboratory Medicine, Keck School of Medicine of the University of Southern California, USC Norris Cancer Center, NRT 7514, 1450 Biggy Street, Los Angeles, CA 90089 9601, USA
    Infect Immun 79:459-73. 2011
    b>Paneth cells at the base of small intestinal crypts secrete microbicidal α-defensins, termed cryptdins (Crps) in mice, as mediators of innate immunity...
  41. pmc A common role for Atg16L1, Atg5 and Atg7 in small intestinal Paneth cells and Crohn disease
    Ken Cadwell
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Autophagy 5:250-2. 2009
    ..Autophagy-deficient Paneth cells exhibited a striking loss of function in this granule exocytosis pathway...
  42. ncbi A genetic study of the role of the Wnt/beta-catenin signalling in Paneth cell differentiation
    Pauline Andreu
    Institut Cochin, Universite Paris Descartes, CNRS UMR 8104, Paris, France
    Dev Biol 324:288-96. 2008
    ..We demonstrated that acute activation of Wnt/beta-catenin signalling induces de novo specification of Paneth cells in both the small intestine and colon and that colon cancers resulting from Apc mutations expressed many genes ..
  43. pmc Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis
    Y Ogura
    Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Gut 52:1591-7. 2003
    ..The reason for the unique association of NOD2 mutations with ileal disease is unclear. To identify a possible link, we tested expression of NOD2 in intestinal tissue of CD patients and controls...
  44. pmc Developmental regulation of intestinal angiogenesis by indigenous microbes via Paneth cells
    Thaddeus S Stappenbeck
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 99:15451-5. 2002
    ..b>Paneth cells in the intestinal epithelium secrete antibacterial peptides that affect luminal microbial ecology...
  45. ncbi Angiogenins: a new class of microbicidal proteins involved in innate immunity
    Lora V Hooper
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Nat Immunol 4:269-73. 2003
    ..We show that a previously uncharacterized angiogenin, Ang4, is produced by mouse Paneth cells, is secreted into the gut lumen and has bactericidal activity against intestinal microbes...
  46. ncbi Crypt-restricted proliferation and commitment to the Paneth cell lineage following Apc loss in the mouse intestine
    Pauline Andreu
    Institut Cochin, INSERM U567, CNRS UMR8104, Universite Paris V, 24 rue du Fb St Jacques, 75014 Paris, France
    Development 132:1443-51. 2005
    ..along the enterocyte, goblet and enteroendocrine lineages, and promotes commitment to the Paneth cell lineage through beta-catenin/Tcf4-mediated transcriptional control of specific markers of Paneth cells, the cryptdin/defensin genes.
  47. ncbi PPARbeta/delta regulates paneth cell differentiation via controlling the hedgehog signaling pathway
    Frédéric Varnat
    Center for Integrative Genomics, National Research Centre Frontiers in Genetics, University of Lausanne, Lausanne, Switzerland
    Gastroenterology 131:538-53. 2006
    ..However, its function in intestinal epithelium homeostasis is understood poorly...
  48. ncbi Maturation of paneth cells induces the refractory state of newborn mice to Shigella infection
    MARIA ISABEL FERNANDEZ
    Unite de Pathogenie Microbienne Moleculaire, Département de Biologie Cellulaire et Infection, Unité Institut National de la Santé et de la Recherche Médicale U786, Paris, France
    J Immunol 180:4924-30. 2008
    ..Indeed, expression of genes specific of Paneth cells was higher in 7-day- than in 4-day-old mice, and histological analysis indicated that Paneth cells were present ..
  49. ncbi Defective paneth cell-mediated host defense in pediatric ileal Crohn's disease
    Gøri Perminow
    Department of Pediatrics, Akershus University Hospital and Oslo University Hospital, Oslo, Norway
    Am J Gastroenterol 105:452-9. 2010
    ..The aim of this study was to evaluate human alpha-defensin-5 (HD-5) and TCF-4 in an independent cohort of pediatric CD patients...
  50. pmc Cdx2 levels modulate intestinal epithelium maturity and Paneth cell development
    Mary Ann S Crissey
    Division of Gastroenterology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Gastroenterology 140:517-528.e8. 2011
    ..We generated transgenic mice that overexpress Cdx2 in the small intestinal and colonic epithelium to investigate the role of Cdx2 in differentiation and function of the intestinal epithelium...
  51. ncbi Requirement of Math1 for secretory cell lineage commitment in the mouse intestine
    Q Yang
    Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Science 294:2155-8. 2001
    ..of four principal cell types deriving from one multipotent stem cell: enterocytes, goblet, enteroendocrine, and Paneth cells. Previous studies showed that Math1, a basic helix-loop-helix (bHLH) transcription factor, is expressed in the ..
  52. ncbi Regulation of intestinal alpha-defensin activation by the metalloproteinase matrilysin in innate host defense
    C L Wilson
    Department of Pediatrics, Division of Allergy and Pulmonary Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 286:113-7. 1999
    ..Thus, matrilysin functions in intestinal mucosal defense by regulating the activity of defensins, which may be a common role for this metalloproteinase in its numerous epithelial sites of expression...
  53. pmc Paneth cells and antibacterial host defense in neonatal small intestine
    Michael P Sherman
    Department of Pediatrics, University of California, Davis, CA, USA
    Infect Immun 73:6143-6. 2005
    b>Paneth cells are specialized epithelia in the small bowel that secrete antimicrobial proteins...
  54. pmc Starvation compromises Paneth cells
    Caroline M Hodin
    Department of Surgery, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center, Maastricht, The Netherlands
    Am J Pathol 179:2885-93. 2011
    ..Recently, Paneth cells, important constituents of innate intestinal immunity, were found to be crucial in host protection against ..
  55. ncbi In vivo killing of Giardia trophozoites harbouring bacterial endosymbionts by intestinal Paneth cells: an ultrastructural study
    K A El-Shewy
    Microbiology and Parasitology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
    Parasitology 130:269-74. 2005
    To date Paneth cells have not previously been reported to kill Giardia trophozoites and other protozoa in vivo. Here we report the first evidence for in vivo killing of Giardia trophozoites by intestinal Paneth cells...
  56. ncbi Abnormal activation of autophagy-induced crinophagy in Paneth cells from patients with Crohn's disease
    Elodie Thachil
    Univ Paris Diderot, Sorbonne Paris Cité, France
    Gastroenterology 142:1097-1099.e4. 2012
    ..We observed that autophagy was specifically activated in Paneth cells from patients with CD, independently of mucosal inflammation or disease-associated variants of ATG16L1 or IRGM...
  57. pmc Host genes related to paneth cells and xenobiotic metabolism are associated with shifts in human ileum-associated microbial composition
    Tianyi Zhang
    Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, New York, United States of America
    PLoS ONE 7:e30044. 2012
    ..The results of permutation based multivariate analysis of variance and covariance (MANCOVA) support the hypothesis that host mucosal Paneth cell and xenobiotic metabolism genes play an important role in host microbial interactions...
  58. pmc Paneth cell granule depletion in the human small intestine under infective and nutritional stress
    P Kelly
    Institute of Cell and Molecular Science, Bart s and the London School of Medicine, London, UK
    Clin Exp Immunol 135:303-9. 2004
    b>Paneth cells are important contributors to the intestinal antimicrobial barrier through synthesis and release of antimicrobial peptides and proteins...
  59. pmc Paneth cells and necrotizing enterocolitis
    Mark A Underwood
    Department of Pediatrics, UC Davis School of Medicine, Sacramento, CA, USA
    Gut Microbes 3:562-5. 2012
    ..Recent studies suggest a key role for Paneth cells in this disease...
  60. pmc Paneth cells expand from newly created and preexisting cells during repair after doxorubicin-induced damage
    Stephanie L King
    Department of Surgery, University of North Carolina, Chapel Hill, NC 27599, USA
    Am J Physiol Gastrointest Liver Physiol 305:G151-62. 2013
    ..b>Paneth cells were birth dated using incorporation of thymidine analogs given before or after Dox...
  61. pmc Expansion of Paneth cell population in response to enteric Salmonella enterica serovar Typhimurium infection
    Nadine R Martinez Rodriguez
    Department of Biological Sciences, California State University Los Angeles, Los Angeles, California, USA
    Infect Immun 80:266-75. 2012
    b>Paneth cells residing at the base of the small intestinal crypts contribute to the mucosal intestinal first line defense by secreting granules filled with antimicrobial polypeptides including lysozyme...
  62. pmc Interconversion between intestinal stem cell populations in distinct niches
    Norifumi Takeda
    Department of Cell and Developmental Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 334:1420-4. 2011
    ..Conversely, CBCs can give rise to +4 Hopx-positive cells. These findings demonstrate a bidirectional lineage relationship between active and quiescent stem cells in their niches...
  63. ncbi Enhanced expression of transforming growth factor (TGF) -alpha precursor and TGF-beta1 during Paneth cell regeneration
    H Seno
    Department of Internal Medicine, Kyoto University, Graduate School of Medicine, Japan
    Dig Dis Sci 46:1004-10. 2001
    ..of diphenylthiocarbazone (dithizone), a zinc chelator, induces selective killing and rapid regeneration of Paneth cells, which have a large amount of zinc in their cytoplasmic granules...
  64. ncbi Level of activation of the unfolded protein response correlates with Paneth cell apoptosis in human small intestine exposed to ischemia/reperfusion
    Joep Grootjans
    Department of Surgery, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Center, Maastricht, The Netherlands
    Gastroenterology 140:529-539.e3. 2011
    In the intestine, Paneth cells participate in the innate immune response. Their highly secretory function makes them susceptible to environmental conditions that cause endoplasmic reticulum (ER) stress...
  65. pmc Cd1d-dependent regulation of bacterial colonization in the intestine of mice
    Edward E S Nieuwenhuis
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 119:1241-50. 2009
    ..Together, these data support a role for Cd1d in regulating intestinal colonization through mechanisms that include the control of Paneth cell function...
  66. ncbi Zinc-secreting Paneth cells studied by ZP fluorescence
    Leonard J Giblin
    NeuroBioTex, Inc, Galveston, TX 77550, USA
    J Histochem Cytochem 54:311-6. 2006
    ..family of zinc-specific-responsive fluorescent dyes (ZPs) to study the sequestration and secretion of zinc from Paneth cells, which are located in the bases of the crypts of Lieberkühn within the rat small intestine...
  67. ncbi Defensins and Paneth cells in inflammatory bowel disease
    Jishu Shi
    Department of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, Alabama 36849, USA
    Inflamm Bowel Dis 13:1284-92. 2007
    Defensins are antimicrobial peptides produced by professional phagocytes, Paneth cells, and intestinal epithelial cells...
  68. ncbi Enteropathy with loss of enteroendocrine and paneth cells in a patient with immune dysregulation: a case of adult autoimmune enteropathy
    Hisham Al Khalidi
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada
    Hum Pathol 37:373-6. 2006
    ..The duodenal biopsies showed villous blunting, chronic inflammation, and decreased to absent goblet cells, Paneth cells, and endocrine cells by histology and electron microscopy...
  69. pmc Autophagy gives a nod and a wink to the inflammasome and Paneth cells in Crohn's disease
    Vojo Deretic
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 915 Camino de Salud, The University of New Mexico, Northeast Albuquerque, NM 87131, USA
    Dev Cell 15:641-2. 2008
    ..Now, experiments with Atg16L1 transgenic mice indicate multiple roles for autophagy in inflammatory bowel disease via effects on Paneth cells, a runaway inflammasome, and the proinflammatory cytokine IL-1beta.
  70. ncbi Paneth cell hyperplasia and metaplasia in necrotizing enterocolitis
    Patrycja J Puiman
    Department of Neonatology, Erasmus MC Sophia Children s Hospital, Rotterdam 3015 GJ, The Netherlands
    Pediatr Res 69:217-23. 2011
    ..In the colon, metaplastic Paneth cells were observed. Ileostomy fluid contained Paneth cell proteins and inhibited bacterial growth...
  71. pmc Sorting mouse jejunal epithelial cells with CD24 yields a population with characteristics of intestinal stem cells
    Richard J von Furstenberg
    Dept of Medicine, University of North Carolina at Chapel Hill, 27599 7555, USA
    Am J Physiol Gastrointest Liver Physiol 300:G409-17. 2011
    ..Flow cytometry with anti-lysozyme antibodies demonstrated that Paneth cells comprise ∼30% of the CD24(lo) subfraction...
  72. ncbi SOX9 is required for the differentiation of paneth cells in the intestinal epithelium
    Yuko Mori-Akiyama
    Department of Molecular Geneticsm, The University of Texas, M D Anderson Cancer Center, Houston, Texas, USA
    Gastroenterology 133:539-46. 2007
    ..In the intestine, Sox9 is expressed in the epithelial cells of the crypts and is a target of Wnt signaling...
  73. pmc Distinct SOX9 levels differentially mark stem/progenitor populations and enteroendocrine cells of the small intestine epithelium
    Eric J Formeister
    Department of Medicine, Division of Gastroenterology and Hepatology, University of North Carolina at Chapel Hill, NC 27599, USA
    Am J Physiol Gastrointest Liver Physiol 296:G1108-18. 2009
    ..These data support a bimodal role for SOX9 in the intestinal epithelium, where low SOX9 expression supports proliferative capacity, and high SOX9 expression suppresses proliferation...
  74. ncbi IL-9 promotes IL-13-dependent paneth cell hyperplasia and up-regulation of innate immunity mediators in intestinal mucosa
    Valérie Steenwinckel
    Ludwig Institute for Cancer Research, Brussels Branch, Brussels, Belgium
    J Immunol 182:4737-43. 2009
    ..Histochemical staining of Paneth cells by phloxine/tartrazine showed that IL-9 induces Paneth cell hyperplasia in Lieberkühn glands of the small ..
  75. ncbi Cell Lineage metastability in Gfi1-deficient mouse intestinal epithelium
    Matthew Bjerknes
    Department of Medicine, Clinical Science Division, Medical Sciences Building, Room 6334, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada M5S 1A8
    Dev Biol 345:49-63. 2010
    ....
  76. ncbi Crohn's disease and the NOD2 gene: a role for paneth cells
    Sanjay Lala
    Department of Medicine, Royal Free and University College Medical School, London, United Kingdom
    Gastroenterology 125:47-57. 2003
    ..b>Paneth cells on the other hand, are most numerous in the terminal ileum, are critically important in enteric antibacterial ..
  77. ncbi Cell migration and organization in the intestinal crypt using a lattice-free model
    F A Meineke
    Institute for Medical Informatics, Statistics and Epidemiology, Leipzig, Germany
    Cell Prolif 34:253-66. 2001
    ....
  78. ncbi Cloning of the novel gene intelectin, which is expressed in intestinal paneth cells in mice
    T Komiya
    Hirohashi Cell Configuration Project, ERATO, Japan Science and Technology Corporation JST, 5 9 4 Tokodai, Tsukuba, 300 26, Japan
    Biochem Biophys Res Commun 251:759-62. 1998
    ..screening method, we isolated the cDNA gene intelectin, whose mRNA is expressed in small intestinal paneth cells in mice. Northern blot analysis revealed that the mRNA corresponding to the cDNA was 1...
  79. pmc Genetic variants of Wnt transcription factor TCF-4 (TCF7L2) putative promoter region are associated with small intestinal Crohn's disease
    Maureen J Koslowski
    Dr Margarete Fischer Bosch Institute of Clinical Pharmacology, University of Tubingen, Stuttgart, Germany
    PLoS ONE 4:e4496. 2009
    ..38, 95% CI 1.03 to1.84, p = 0.02882). The newly identified genetic association of TCF-4 with ileal CD provides evidence that the decrease in Paneth cell alpha-defensins is a primary factor in disease pathogenesis...
  80. ncbi Influence of alemtuzumab on the intestinal Paneth cells and microflora in macaques
    Qiurong Li
    Research Institute of General Surgery, Jinling Hospital, Nanjing 210002, China School of Medicine, Nanjing University, Nanjing 210093, China
    Clin Immunol 136:375-86. 2010
    ..We found the alterations of Paneth cells including abnormal Paneth cell granules and expression of lysozyme and defensin 5 in response to lymphocyte ..
  81. ncbi Immunocytochemical localization of secretory component in Paneth cell secretory granules-rat Paneth cells participate in acquired immunity
    Qing Juan Tang
    Department of Nautical Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, P R China
    J Mol Histol 36:331-5. 2005
    With the marker of Paneth cells-lysozyme, secretory component (SC) immunoreactivity was demonstrated exclusively in Paneth cells of rat small intestine. The other types of epithelial cells (columnar, goblet, endocrine) were negative...
  82. pmc Colony stimulating factor-1 dependence of paneth cell development in the mouse small intestine
    Duy Huynh
    Peter MacCallum Cancer Centre, East Melbourne, Australia
    Gastroenterology 137:136-44, 144.e1-3. 2009
    b>Paneth cells (PCs) secrete defensins and antimicrobial enzymes that contribute to innate immunity against pathogen infections within the mucosa of the small intestine...
  83. ncbi Candidate markers for stem and early progenitor cells, Musashi-1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine
    Takahisa Kayahara
    Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54 Shogoin Kawara cho, Sakyo ku, Kyoto 606 8507, Japan
    FEBS Lett 535:131-5. 2003
    ..Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt, the putative position of stem cells, whereas Hes1 is expressed in lower crypt ..
  84. pmc Alpha-defensins in enteric innate immunity: functional Paneth cell alpha-defensins in mouse colonic lumen
    Jennifer R Mastroianni
    Department of Pathology and Laboratory Medicine, University of California, Irvine, California 92697 4800, USA
    J Biol Chem 284:27848-56. 2009
    b>Paneth cells are a secretory epithelial lineage that release dense core granules rich in host defense peptides and proteins from the base of small intestinal crypts...
  85. pmc IL-17 produced by Paneth cells drives TNF-induced shock
    Nozomi Takahashi
    Rheumatology Research and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, Netherlands
    J Exp Med 205:1755-61. 2008
    ..Surprisingly, electron microscopy showed that IL-17 was constitutively present in Paneth cells of the crypts...
  86. ncbi Hedgehog, paneth cells, and colon cancer: a cautionary note for the use of systemic agonists/antagonists
    Charles W Lees
    Gastroenterology 131:1657-8; author reply 1658. 2006
  87. ncbi Paneth cells and the innate immune response
    Jan Wehkamp
    Robert Bosch Hospital and Dr Margarete Fischer Bosch Institute of Clinical Pharmacology, Stuttgart, Germany
    Curr Opin Gastroenterol 22:644-50. 2006
    To review recently published studies presenting novel and relevant information on Paneth cells and their function.
  88. pmc NOD2 mutations and Crohn's disease: are Paneth cells and their antimicrobial peptides the link?
    M C Grimm
    Gastroenterology Unit, Canberra Hospital, Woden ACT 2606, Canberra, Australia
    Gut 53:1558-60. 2004
  89. ncbi Paneth cells: histochemical and morphometric study in control and Solanum glaucophyllum intoxicated rabbits
    C N Zanuzzi
    Institute of Pathology Prof Dr Bernardo Epstein, School of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
    Eur J Histochem 52:93-100. 2008
    ..Using and established rabbit model, this study analyses the changes that rabbit Paneth cells undergo during intoxication with S. glaucophyllum...
  90. ncbi Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues
    Jan Wehkamp
    Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, One Shields Avenue, Tupper Hall Room 3146, 95616 8645, USA
    FEBS Lett 580:5344-50. 2006
    ..In the human small intestine, Paneth cells are secretory epithelial cells that express the antimicrobials human alpha-defensin-5 (HD5), HD6, lysozyme and ..
  91. ncbi Immunology. Unraveling gut inflammation
    Warren Strober
    Laboratory of Host Defenses, National Institutes of Health Bethesda, MD 20892 1890 USA
    Science 313:1052-4. 2006
  92. pmc Human defensin 5 is stored in precursor form in normal Paneth cells and is expressed by some villous epithelial cells and by metaplastic Paneth cells in the colon in inflammatory bowel disease
    R N Cunliffe
    Division of Gastroenterology, University of Nottingham, UK
    Gut 48:176-85. 2001
    ..Our aims were to (i) isolate, characterise, and investigate the processing of human defensin 5 (HD-5) in normal Paneth cells and (ii) investigate expression of HD-5 in active inflammatory bowel disease (IBD)...
  93. ncbi The role of Paneth cells and their antimicrobial peptides in innate host defense
    Tokiyoshi Ayabe
    Department of Internal Medicine III, Asahikawa Medical College, 2 1 1 1 Midorigaoka Higashi, Asahikawa 078 8510, Japan
    Trends Microbiol 12:394-8. 2004
    ..b>Paneth cells, one of four major epithelial cell lineages in the small intestine, reside at the base of the crypts and have ..
  94. ncbi Increased diversity of intestinal antimicrobial peptides by covalent dimer formation
    Mathias W Hornef
    Institute of Medical Microbiology and Hygiene, University of Freiburg, D 79104 Freiburg, Germany
    Nat Immunol 5:836-43. 2004
    ..CRS peptides might therefore be important in the maintenance of the microbial homeostasis within the intestinal tract...
  95. ncbi The bHLH transcription factor Tcf12 (ME1) mRNA is abundantly expressed in Paneth cells of mouse intestine
    Yoko Tanigawa
    Department of Biological Function, Graduate School of Science, Osaka City University, Sumiyoshi, Osaka, Japan
    Gene Expr Patterns 7:709-13. 2007
    ..that mRNA coding for ME1, a basic helix-loop-helix (bHLH) transcription factor, was abundantly expressed in Paneth cells of adult small intestinal crypts...
  96. pmc Degranulation of paneth cells via toll-like receptor 9
    Cristiano Rumio
    Department of Human Morphology, University of Milan, Italy
    Am J Pathol 165:373-81. 2004
    The release of antimicrobial peptides and growth factors by Paneth cells is thought to play an important role in protecting the small intestine, but the mechanisms involved have remained obscure...
  97. ncbi Paneth cells: leukocyte-like mediators of innate immunity in the intestine
    Satish Keshav
    Department of Medicine, Centre for Gastroenterology, Royal Free and University College Medical School, UCL, Rowland Hill Street, London, NW3 2PF, UK
    J Leukoc Biol 80:500-8. 2006
    b>Paneth cells are secretory intestinal epithelial cells located at the base of the crypts of Lieberkühn in the small intestine...
  98. ncbi Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine
    Katsumasa Suzuki
    Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan
    Biochem Biophys Res Commun 328:348-52. 2005
    We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells...
  99. pmc Peptide localization and gene structure of cryptdin 4, a differentially expressed mouse paneth cell alpha-defensin
    A J Ouellette
    Department of Pathology, College of Medicine, University of California, Irvine, California 92697, USA
    Infect Immun 67:6643-51. 1999
    b>Paneth cells in crypts of the small intestine express antimicrobial peptides, including alpha-defensins, termed cryptdins in mice...
  100. ncbi Identification of Paneth cells in pyloric glands associated with gastric and intestinal mixed-type intestinal metaplasia of the human stomach
    K Inada
    Laboratory of Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan
    Virchows Arch 439:14-20. 2001
    ..type, gastric (foveolar epithelial and pyloric gland cells) and intestinal (goblet, intestinal absorptive, and Paneth cells) phenotype cells coexist in the same intestinalized gastric glands in various combinations and degrees...
  101. pmc Paneth cells: their role in innate immunity and inflammatory disease
    D A Elphick
    Institute of Infection, Immunity, and Inflammation, C Floor, West Block, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Gut 54:1802-9. 2005

Research Grants63

  1. Route of Nutrition and Enteric Nervous System Effects on Innate Mucosal Defense
    Kenneth A Kudsk; Fiscal Year: 2013
    ..The Paneth cells are the source of an important innate immune molecule, secretory phospholipase A2 (sPLA2)...
  2. Mechanisms of host-bacterial interactions, ileitis and granuloma formation in Agr
    Steven M Lipkin; Fiscal Year: 2013
    ..Intestinal goblet, Paneth cells and macrophages have increased ER stress...
  3. Intestinal Stem Cell Survival and Renewal Coordinately Regulated by PUMA and p21
    Jian Yu; Fiscal Year: 2013
    ..Genetic evidence demonstrated that the crypt-based columnar cells (CBCs) interspersed among Paneth cells marked by Lgr5 and some +4 cells immediately above Paneth cells marked by Bmi-1, represent perhaps distinct ..
  4. SUSCEPTIBILILTY GENE-VIRUS INTERACTIONS IN INFLAMMATORY BOWEL DISEASE
    Herbert W Virgin; Fiscal Year: 2013
    ..express low levels of Atg16L1 protein (hypomorphic, Atg16L1HM) have a striking abnormality of ileal epithelial Paneth cells including abnormal secretion of granules containing anti-microbial peptides1...
  5. Genetic Control of Intestinal Epithelial Stem Cell Maintenance &Differentiation
    Scott T Magness; Fiscal Year: 2013
    ..that distinct levels of Sox9 differentially mark functional IESCs, progenitors, enteroendocrine cells and Paneth cells. Sox9 and Lgr5 both mark IESCs that are intercalated between and make intimate contact with Paneth cells...
  6. Role of Paneth Cells in Development of Necrotizing Enterocolitis
    STEVEN JAMES MCELROY; Fiscal Year: 2013
    ..b>Paneth cells protect the intestine through secretion of pro-inflammatory mediators and antimicrobial substances...
  7. Defensins in STI-Mediated Enhanced HIV Infectivity
    Theresa L Chang; Fiscal Year: 2013
    ..5 and 6 (HD5 and HD6), the most abundant antimicrobial peptides in intestine, are constitutively expressed in Paneth cells but also found in the epithelium of the vagina and ectocervix...
  8. Regulation of the Intestinal Stem Cell Niche in Aging
    Omer Yilmaz; Fiscal Year: 2013
    ..In the mammalian intestine, a majority of ISCs express Lgr5 and are adjacent to Paneth cells, which constitute a component of the stem cell cellular neighborhood or "niche"...
  9. Mechanisms of Intestinal Stem Cell Expansion Following Resection
    Michael A Helmrath; Fiscal Year: 2013
    ..suggest that there may be two populations of ISC: a slowly cycling quiescent-ISC pool (q-ISC) located above Paneth cells (Upper Stem cell Zone, USZ) and a more rapidly cycling active-ISC pool (a-ISC) of crypt base columnar cells (..
  10. DETERMINANTS OF ALPHA DEFENSIN RESISTANCE IN THE INTESTINAL MICROBIOTA
    CHRISTINE TZY YUH LUO; Fiscal Year: 2013
    ..b>Paneth cells are an intestinal epithelial cell type tha plays a significant role in shaping the microbiota by secreting ..
  11. Innate enteric immunity during induced Paneth cell deficiency
    Andre J Ouellette; Fiscal Year: 2013
    ..Anatomically restricted to the small bowel, Paneth cells release [unreadable]-defensins and host defense molecules, and maintenance of Paneth cell homeostasis is ..
  12. Nod2: A Susceptibility Gene for Crohn's Disease
    Gabriel Nunez; Fiscal Year: 2013
    ..suggesting that Nod2 plays a critical role in the regulation of ileal immunity Nod2 is highly expressed in Paneth cells, a specialized cell population located in the crypts that produces anti-microbial peptides...
  13. Cell Growth Signaling in Cancer Development
    David M Sabatini; Fiscal Year: 2013
    ..mTORC1 acts in Paneth cells, which constitute the niche for ISCs and are located at the base of intestinal crypts...
  14. Understanding the mechanisms that govern Bst-1 induction upon caloric restriction
    Shuyu Wang; Fiscal Year: 2012
    ..ISCs through a non-cell autonomous mechanism requiring the inhibition of mTORC1 and the upregulation of Bst1 in Paneth cells. Surprisingly, Paneth cells transitioned from CR conditions to nutrient-rich conditions maintained the CR ..
  15. New Mouse Models of Paneth Cell Defensin Function
    Charles L Bevins; Fiscal Year: 2013
    ..mechanisms that regulate this delicate balance is lacking, but specialized secretory epithelial cells, called Paneth cells, appear to be important contributors...
  16. Immunogenetic Mechanisms of Experimental Crohn's Disease
    Steven M Cohn; Fiscal Year: 2011
    ..Project 3, headed by Dr. Steven Cohn, will investigate the role of Paneth cells and their defensins in initiating ileitis. Project 4, headed by Dr...
  17. REGULATION OF ADENOSINE DEAMINASE IN SMALL INTESTINE
    Dan Wiginton; Fiscal Year: 2006
    ..Enterocytes, goblet cells, and enteroendocrine cells migrate up along the villus, while Paneth cells migrate down into the crypt...
  18. Functional and molecular characteristics of Paneth cells during injury and repair
    Christopher M Dekaney; Fiscal Year: 2013
    ..b>Paneth cells reside at the base of small intestinal crypts, and intercalated among them are ISC...
  19. Defensins in Innate Immunity to Viruses
    Jason G Smith; Fiscal Year: 2012
    ..We will also determine whether host Paneth cells respond to virus infection by degranulating and releasing alpha-defensins into the gut...
  20. HUMAN DEFENSIN 5 AND INTESTINAL COLONIZATION
    Nita Salzman; Fiscal Year: 2000
    ..The human enteric defensins are produced by Paneth cells at the bases of the small intestinal crypts...
  21. Cellular targets of IL-4 and IL-13 in worm expulsion
    Fred Finkelman; Fiscal Year: 2007
    ..Both IL-4 and IL-13 affect non-bone marrow-derived cells, including intestinal epithelium, goblet cells, Paneth cells, smooth muscle, and vascular endothelium, in ways that might contribute to worm expulsion from the gut; however,..
  22. New Mechanisms in the Pathogenesis of Cholera
    JOHNNY PETERSON; Fiscal Year: 2006
    ..to CT will be identified using in situ hybridization and immunohistochemistry, and the role of PLAA and Paneth cells will be established with PLAA and Paneth cell knockout mice...
  23. Gastric and intestinal epithelial progenitor cell GAP
    Jeffrey Gordon; Fiscal Year: 2004
    ..another model, the representation of intestinal epithelial progenitors is augmented markedly through removal of Paneth cells from the base of crypts of Lieberkuhn...
  24. Paneth Cell Cryptdins in Innate Intestinal Host Defense
    Patricia Lin; Fiscal Year: 2007
    ..b>Paneth cells, located at the base of intestinal crypts produce intestinal-specific alpha-defensins, termed cryptdins, which ..
  25. In vivo Models of Defensin Activity
    CHARLES BEVINS; Fiscal Year: 2006
    ..The HD6 gene and peptide share little sequence identity to HD5, yet they are expressed together in Paneth cells. In Aim 2, we will generate HD6 transgenic mice (Aim 2A) use recombinant HD6 to develop an antibody for ..
  26. CRYPTDIN EFFECTS ON CRYPT EPITHELIA
    Wayne Lencer; Fiscal Year: 2004
    ..Cryptdins, and HD-5 and HD-6 are known to be released from Paneth cells into the crypt lumen and thus other crypt cell types, the major one being the chloride secreting "..
  27. DIFFERENTIATION OF ENDOCRINE CELL LINEAGES IN GI TRACT
    Andrew Leiter; Fiscal Year: 2001
    ..mucosa arise from stem cells and differentiate along four possible pathways into enterocytes, goblet cells, Paneth cells, or enteric endocrine cells. The signals for this differentiation are largely unknown...
  28. ZINC AND THE SYNTHESIS OF ZINC BINDING PROTEINS
    Robert Cousins; Fiscal Year: 2009
    ..The zinc processing steps the pancreatic acinar cells and Paneth cells use for endogenous zinc release will receive close attention...
  29. MOLECULAR MODE OF ACTION OF BACTERIAL ENTEROTOXINS
    JOHNNY PETERSON; Fiscal Year: 2001
    ..Intestinal cells (e.g., Paneth cells) forming PLAA in response to CT will be identified using in situ hybridization and immunohistochemistry...
  30. LYSOZYME SECRETION AND PANETH CELL FUNCTION
    Stanley Erlandsen; Fiscal Year: 1980
    ..The secretion of lysozyme and the lysozyme content of Paneth cells in vivo and in vitro will be quantitated by radioimmunoassay-immunocytochemical techniques and correlated with: ..
  31. Regulation Paneth cell defensins by bacterial infection
    Nita Salzman; Fiscal Year: 2004
    ..In the small intestinal crypts, highly secretory Paneth cells are the primary source of antimicrobial peptides...
  32. IMMUNOLOGIC ASPECTS OF PANETH CELL FUNCTION
    I Wilson; Fiscal Year: 1980
    b>Paneth cells are a common cell type in human small intestine, located at the base of most, if not all, crypts. Despite their prevalence, very little is known about their function...
  33. MECHANISTIC ANALYSIS OF DEFENSIN MICROBICIDAL ACTIVITY
    DONALD SATCHELL; Fiscal Year: 2002
    The central hypothesis of this research is that gene encoded microbicidal peptides released by Paneth cells contribute to innate immunity in the small intestine...
  34. Genetic Studies in Crohn's Disease
    Jean Paul Achkar; Fiscal Year: 2009
    ..similar to findings with NOD2/CARD15 variants, abnormalities in innate host defense involving Paneth cells account for susceptibility to CD in different patient subgroups...
  35. 2005 Antimicrobial Peptides Gordon Conference
    Andre Ouellette; Fiscal Year: 2005
    ..The organizational philosophy is to encourage diverse participation, and maintain an eye towards gender issues, junior scientists, minorities, and individuals with physical limitations. ..
  36. Regulation Paneth cell defensins by bacterial infection
    Nita Salzman; Fiscal Year: 2009
    ..In the small intestinal crypts, highly secretory Paneth cells are the primary source of antimicrobial peptides and other effector molecules...
  37. STUDIES OF ALPHA-DEFENSINS IN PRIMATE INNATE IMMUNITY
    Andre J Ouellette; Fiscal Year: 2010
    ..of action and the structural basis of that activity, investigate mechanisms of proRED and proRMAD activation in Paneth cells and myeloid cells, and identify cis-acting structural components of RMAD and RED genes that regulate the ..
  38. PEPTIDE EFFECTORS OF ENTERIC HOST DEFENSE
    Andre J Ouellette; Fiscal Year: 2010
    ..The processing and activation mechanisms of proCRSIC and proCRS4C in mouse Paneth cells will be identified, and the structures of CRS4C and CRS1C in solution and in bicelles will be determined by NMR ..
  39. Lipids: Effectors in Innate Immunity
    Edith Porter; Fiscal Year: 2005
    ..abstract_text> ..
  40. Mechanism of M. tuberculosis Phagosome Maturation Arrest
    Vojo Deretic; Fiscal Year: 2008
    ..tuberculosis and provide a foundation for new interventions potentially combating both active disease and latent infection. ..
  41. DEFENSINS--ANTIMICROBIAL PEPTIDES OF HUMAN NEUTROPHILS
    Robert Lehrer; Fiscal Year: 2002
    ..The work with murine neutrophils, whose content of endogenous antimicrobial polypeptides differs greatly from those of humans, will provide essential information relevant to the interpretation of murine models of human infection. ..
  42. Biacore 3000 SPR Instrument
    Robert Lehrer; Fiscal Year: 2004
    ..These include the development of new agents and approaches to prevent and treat infections caused by HIV-1 and other viral pathogens, and fundamental issues related to cell targeting and survival. ..
  43. ALPHA HELICAL CATHELICIDINS AND HOST DEFENSE
    Robert Lehrer; Fiscal Year: 2003
    ..The experiments will expand our knowledge about these important peptides, and should provide the information needed to develop them as antibiotics for topical bronchopulmonary use in cystic fibrosis. ..
  44. Theta-defensins Novel HIV-1 Uptake Inhibitors
    Robert Lehrer; Fiscal Year: 2007
    ..Specific Aim 2 will be realized by a combination of microchemical techniques, including surface plasmon resonance, tandem mass spectrometry, and state-of-the-art lectinomic and peptidomic chemistry. ..
  45. ALTRUINS, NATURAL ANTIBIOTICS OF THE MALE GENITAL TRACT
    Robert Lehrer; Fiscal Year: 2001
    ..S. are sexually transmitted. According to CDC estimates, STDs other than AIDS add 10 billion dollars to the nation's annual health care I costs. The proposed studies could lead to novel approaches for STD prophylaxis. ..
  46. STRUCTURE/FUNCTIONS OF HUMAN TEAR LIPOPHILIN
    Robert Lehrer; Fiscal Year: 2001
    ..The long-term objectives for this research are to learn how lipophilin contributes to corneal and conjuctival health, and to explore its potential for creating new diagnostic and therapeutic modalities. ..
  47. Retrocyclin reinforcement of pulmonary defenses against viral aerosols
    Robert Lehrer; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  48. Hyperacidification and Pseudomonas infections in CF
    Vojo Deretic; Fiscal Year: 2006
    ..abstract_text> ..
  49. Autophagy in Tuberculosis
    VOJO P DERETIC; Fiscal Year: 2011
    ..Our studies will provide novel pharmacological and immunological approaches in treatment and prophylaxis of tuberculosis, with strong implications for immunity and infectious diseases in general. ..
  50. Pseudomonas in Cystic Fibrosis-Regulation of Mucoidy
    Vojo Deretic; Fiscal Year: 2006
    ..These studies are expected to improve our understanding of respiratory pathogenesis in CF, and lead to new treatments of presently incurable lung infections associated with this disease...
  51. Retrocyclins: Cyclic mini-defensins that inactivate anthrax toxins
    ROBERT IRVING contact LEHRER; Fiscal Year: 2010
    ..anthracis spores in mouse models as per the revised Specific Aims. All remaining Aims can be addressed in a two-year time period. PHS ..
  52. Bile acid-induced colon cancer cell proliferation
    Jean Pierre Raufman; Fiscal Year: 2009
    ..3c) The mechanism whereby bile acids activate metalloproteases will be determined by exploring the roles of PKC, Ca2+, and Src in bile acid-induced HB-EGF release. ..
  53. IMMUNE PROTECTION AGAINST MNU-INDUCTED DIGITAL DEFECTS
    MARY PRATER; Fiscal Year: 2006
    ..This research under the guidance of Drs. Steven Holladay and Eric Wong at Virginia Tech...
  54. Cdx2 modulates beta-catenin activity in intestinal cells
    John P Lynch; Fiscal Year: 2010
    ..As a result, potential novel targeted therapeutics mayalso emerge. ..
  55. REGULATION OF INTESTINAL EPITHELIAL CELL PROLIFERATION
    John Lynch; Fiscal Year: 2004
    ..It will also enhance our understanding of the events leading to dysregulation of proliferation and human colon cancers. ..
  56. HOST DEFENSES AGAINST PATHOGENIC E. coli
    Lars Eckmann; Fiscal Year: 2007
    ..coli, thus providing an important basis for designing immunization strategies against these pathogens. ..
  57. MUTANT NOD2 IN INTESTINAL INFLAMMATION AND HOST DISEASE
    Lars Eckmann; Fiscal Year: 2010
    ..Together, the proposed studies are expected to yield important new insights into the physiological functions of mutant NOD2 as a mechanistic basis for designing new strategies in the treatment and prevention of CD. ..
  58. SIGNALS THAT CONTROL MOUSE INTESTINAL DIFFERENTIATION
    Thaddeus Stappenbeck; Fiscal Year: 2005
    ..This approach will allow for a more comprehensive analysis of the effects of genetically ablating Rae I in the developing or fully formed crypt villus unit in the small intestine and crypt-surface cuff unit in the colon. ..
  59. Responses of Intestinal Stem Cells to Epithelial Injury
    Thaddeus Stappenbeck; Fiscal Year: 2005
    ..The resulting datasets will provide a molecular picture of signaling pathways active in colonic epithelial stem cells during wound healing and epithelial-mesenchymal crosstalk that occurs during this physiologic response to injury ..
  60. Novel Defensins of Human Epithelia
    CHARLES BEVINS; Fiscal Year: 2009
    ..In the human small intestine, defensins HD5 and HD6 are highly expressed in secretory epithelial cells called Paneth cells. Investigations by our group, and those of others, provide strong support for the hypothesis that Paneth cell ..