melanosomes

Summary

Summary: Melanin-containing organelles found in melanocytes and melanophores.

Top Publications

  1. ncbi Lysosome-related organelles
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    FASEB J 14:1265-78. 2000
  2. pmc Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes
    Christina Wasmeier
    Molecular and Cellular Medicine, Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, England, UK
    J Cell Biol 175:271-81. 2006
  3. pmc Lysosome-related organelles: driving post-Golgi compartments into specialisation
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Curr Opin Cell Biol 19:394-401. 2007
  4. ncbi Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 3:11-21. 1999
  5. pmc Genetic analysis of lysosomal trafficking in Caenorhabditis elegans
    Greg J Hermann
    Department of Biology, Lewis and Clark College, Portland, OR 97219, USA
    Mol Biol Cell 16:3273-88. 2005
  6. pmc Distinct protein sorting and localization to premelanosomes, melanosomes, and lysosomes in pigmented melanocytic cells
    G Raposo
    Curie Institute, Research Section, Paris, 7505 France
    J Cell Biol 152:809-24. 2001
  7. ncbi Oa1 knock-out: new insights on the pathogenesis of ocular albinism type 1
    B Incerti
    Telethon Institute of Genetics and Medicine, San Raffaele Biomedical Science Park, Via Olgettina 58, I 20132 Milan, Italy
    Hum Mol Genet 9:2781-8. 2000
  8. ncbi Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes
    An Chi
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA
    J Proteome Res 5:3135-44. 2006
  9. pmc The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis
    Guillaume van Niel
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Dev Cell 21:708-21. 2011
  10. pmc Melanosomes--dark organelles enlighten endosomal membrane transport
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris, F 75248 France
    Nat Rev Mol Cell Biol 8:786-97. 2007

Research Grants

  1. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
  2. Imaging Nonlinear Absorption of Biomarkers for Improved Detection of Melanoma
    Warren S Warren; Fiscal Year: 2010
  3. RPE Phenotype and Oxidative Stress
    Janice M Burke; Fiscal Year: 2013
  4. BIOGENESIS OF MELANOSOMES
    Seth J Orlow; Fiscal Year: 2013
  5. Function of calcium exchangers in vetebrate pigmentation
    KEITH CHI CHENG; Fiscal Year: 2010
  6. RECEPTOR MEDIATED ENDOCYTOSIS IN ALVEOLAR MACROPHAGES
    Diane McVey Ward; Fiscal Year: 2012
  7. Hermansky Pudlak Syndrome &melanosome formation
    GRACA GRACA RAPOSO; Fiscal Year: 2012
  8. Functional Amyloid in Melansome Biogenesis
    Michael S Marks; Fiscal Year: 2013
  9. Acquisition of a Cryo-Electron Microscope
    Susan Hafenstein; Fiscal Year: 2012
  10. Biology of Fungal Melanin
    Arturo Casadevall; Fiscal Year: 2013

Detail Information

Publications273 found, 100 shown here

  1. ncbi Lysosome-related organelles
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    FASEB J 14:1265-78. 2000
    ..with a group of cell type-specific compartments referred to as 'lysosome-related organelles', which include melanosomes, lytic granules, MHC class II compartments, platelet-dense granules, basophil granules, azurophil granules, and ..
  2. pmc Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes
    Christina Wasmeier
    Molecular and Cellular Medicine, Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, England, UK
    J Cell Biol 175:271-81. 2006
    ..In addition to mature melanosomes, Rab38 and Rab32 localize to perinuclear vesicles carrying tyrosinase and tyrosinase-related protein 1, ..
  3. pmc Lysosome-related organelles: driving post-Golgi compartments into specialisation
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Curr Opin Cell Biol 19:394-401. 2007
    ..Current research reveals how the products of these genes cooperate to generate LROs and how these otherwise diverse organelles are related by the mechanisms through which they form...
  4. ncbi Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell 3:11-21. 1999
    ..Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery...
  5. pmc Genetic analysis of lysosomal trafficking in Caenorhabditis elegans
    Greg J Hermann
    Department of Biology, Lewis and Clark College, Portland, OR 97219, USA
    Mol Biol Cell 16:3273-88. 2005
    ..genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila...
  6. pmc Distinct protein sorting and localization to premelanosomes, melanosomes, and lysosomes in pigmented melanocytic cells
    G Raposo
    Curie Institute, Research Section, Paris, 7505 France
    J Cell Biol 152:809-24. 2001
    b>Melanosomes and premelanosomes are lysosome-related organelles with a unique structure and cohort of resident proteins. We have positioned these organelles relative to endosomes and lysosomes in pigmented melanoma cells and melanocytes...
  7. ncbi Oa1 knock-out: new insights on the pathogenesis of ocular albinism type 1
    B Incerti
    Telethon Institute of Genetics and Medicine, San Raffaele Biomedical Science Park, Via Olgettina 58, I 20132 Milan, Italy
    Hum Mol Genet 9:2781-8. 2000
    ..retinal pigment epithelium (RPE) and skin melanocytes shows the presence of large pigment granules called giant melanosomes or macromelanosomes...
  8. ncbi Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes
    An Chi
    Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA
    J Proteome Res 5:3135-44. 2006
    ..and eye pigmentation in humans, is synthesized, deposited, and distributed in subcellular organelles termed melanosomes. A comprehensive determination of the protein composition of this organelle has been obstructed by the melanin ..
  9. pmc The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis
    Guillaume van Niel
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Dev Cell 21:708-21. 2011
    ..PMEL-a component of melanocyte LROs (melanosomes)-is sorted to ILVs in an ESCRT-independent manner, where it is proteolytically processed and assembled into ..
  10. pmc Melanosomes--dark organelles enlighten endosomal membrane transport
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris, F 75248 France
    Nat Rev Mol Cell Biol 8:786-97. 2007
    b>Melanosomes are tissue-specific lysosome-related organelles of pigment cells in which melanins are synthesized and stored...
  11. ncbi Linking albinism and immunity: the secrets of secretory lysosomes
    Jane Stinchcombe
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
    Science 305:55-9. 2004
    ..b>Melanosomes also use a lysosome-related organelle to secrete melanin for pigmentation...
  12. pmc Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes
    Daniel Gibbs
    Department of Pharmacology, UCSD School of Medicine, La Jolla, CA 92093 0912, USA
    J Cell Sci 117:6473-83. 2004
    ..In mice with mutant Myo7a, melanosomes in the retinal pigmented epithelium (RPE) are distributed abnormally...
  13. ncbi Slac2-a/melanophilin, the missing link between Rab27 and myosin Va: implications of a tripartite protein complex for melanosome transport
    Mitsunori Fukuda
    Laboratory for Developmental Neurobiology, Brain Science Institute, RIKEN The Institute of Physical and Chemical Research, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    J Biol Chem 277:12432-6. 2002
    ..How myosin Va recognizes its cargo (i.e. melanosomes), however, has remained undetermined over the past decade...
  14. ncbi Identification of an organelle receptor for myosin-Va
    Xufeng S Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Cell Biol 4:271-8. 2002
    ..Moreover, this latter interaction, similar to the ability of myosin-Va to colocalize with melanosomes and influence their distribution in vivo, is absolutely dependent on the presence of exon-F, an alternatively ..
  15. pmc Physiological factors that regulate skin pigmentation
    Yuji Yamaguchi
    Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan
    Biofactors 35:193-9. 2009
    ..Enzymatic components of melanosomes include tyrosinase, tyrosinase-related protein 1, and dopachrome tautomerase, which depend on the functions of ..
  16. pmc Melanosome maturation defect in Rab38-deficient retinal pigment epithelium results in instability of immature melanosomes during transient melanogenesis
    Vanda S Lopes
    Molecular and Cellular Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:3914-27. 2007
    ..synthesis in RPE cells occurs embryonically, it is not clear whether adult RPE cells continue to produce melanosomes. Here, we show that progression from pmel17-positive premelanosomes to tyrosinase-positive mature melanosomes ..
  17. ncbi "Transcription physiology" of pigment formation in melanocytes: central role of MITF
    Jiri Vachtenheim
    University Hospital, Prague, Czech Republic
    Exp Dermatol 19:617-27. 2010
    ..polymeric compound melanin is synthesized within melanocytes in the specialized subcellular organelles, termed melanosomes, which are then transferred to surrounding keratinocytes...
  18. pmc A lumenal domain-dependent pathway for sorting to intralumenal vesicles of multivesicular endosomes involved in organelle morphogenesis
    Alexander C Theos
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Dev Cell 10:343-54. 2006
    ..processes as receptor downregulation, viral budding, and biogenesis of lysosome-related organelles such as melanosomes. We show that the melanosomal protein Pmel17 is sorted into ILVs by a mechanism that is dependent upon lumenal ..
  19. ncbi Reconstruction of Microraptor and the evolution of iridescent plumage
    Quanguo Li
    Beijing Museum of Natural History, 126 Tianqiao South Street, Beijing 100050, People s Republic of China
    Science 335:1215-9. 2012
    ..involved in displays of many extant birds are produced by nanoscale arrays of melanin-containing organelles (melanosomes)...
  20. pmc AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis
    Cedric Delevoye
    Structure and Membrane Compartments, Centre Nationale de la Recherche Scientifique, UMR 144 Institut Curie, Centre de Recherche, Paris F 75248, France
    J Cell Biol 187:247-64. 2009
    ..create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes redistributes to pericentriolar ..
  21. ncbi Comparable photoreactivity of hair melanosomes, eu- and pheomelanins at low concentrations: low melanin a risk factor for UVA damage and melanoma?
    Rachel M Haywood
    RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, Middlesex, UK
    Photochem Photobiol 84:572-81. 2008
    ..Data is lacking of the effects of UVA irradiation on human skin melanosomes of different pigmentation, which have not been extensively degraded by isolation procedures...
  22. pmc Mechanical properties of organelles driven by microtubule-dependent molecular motors in living cells
    Luciana Bruno
    Departamento de Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 1, Ciudad Universitaria, Ciudad de Buenos Aires, Argentina
    PLoS ONE 6:e18332. 2011
    ..Melanophores have pigment organelles or melanosomes that move along microtubules toward their minus and plus end by the action of cytoplasmic dynein and kinesin-2, ..
  23. pmc Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
    Maia Brunstein
    Laboratoire de Photonique et de Nanostructures Centre National de la Recherche Scientifique, Marcoussis, France
    Biophys J 97:1548-57. 2009
    ..Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region...
  24. ncbi A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport
    Molly Strom
    Department of Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, United Kingdom
    J Biol Chem 277:25423-30. 2002
    ..Furthermore, overexpression of this domain in melanocytes results in perinuclear clustering of melanosomes, suggesting that this region is sufficient for interaction with, and perturbation of function of, Rab27a in a ..
  25. pmc Mechanisms of protein delivery to melanosomes in pigment cells
    Anand Sitaram
    Cell and Molecular Biology Graduate Group, Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Physiology (Bethesda) 27:85-99. 2012
    ..endosomal trafficking pathways to partition cargo proteins to unique lysosome-related organelles such as melanosomes. This review describes current models of protein trafficking required for melanosome biogenesis in mammalian ..
  26. ncbi Plumage color patterns of an extinct dinosaur
    Quanguo Li
    Beijing Museum of Natural History, 126 Tianqiao South Street, Beijing 100050, People s Republic of China
    Science 327:1369-72. 2010
    ..Fossil feathers can preserve the morphology of color-imparting melanosomes, which allow color patterns in feathered dinosaurs to be reconstructed...
  27. ncbi MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes
    Toshihiko Hoashi
    Laboratory of Cell Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:14006-16. 2005
    ..We conclude that MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation...
  28. pmc N-terminal domains elicit formation of functional Pmel17 amyloid fibrils
    BRENDA WATT
    Departments of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 284:35543-55. 2009
    Pmel17 is a transmembrane protein that mediates the early steps in the formation of melanosomes, the subcellular organelles of melanocytes in which melanin pigments are synthesized and stored...
  29. pmc Premelanosome amyloid-like fibrils are composed of only golgi-processed forms of Pmel17 that have been proteolytically processed in endosomes
    Dawn C Harper
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6100, USA
    J Biol Chem 283:2307-22. 2008
    Melanin pigments are synthesized within specialized organelles called melanosomes and polymerize on intraluminal fibrils that form within melanosome precursors...
  30. ncbi Mutations in genes encoding melanosomal proteins cause pigmentary glaucoma in DBA/2J mice
    Michael G Anderson
    The Howard Hughes Medical Institute, Bar Harbor, Maine 04609, USA
    Nat Genet 30:81-5. 2002
    ..We hypothesize that IPD and ISA alter melanosomes, allowing toxic intermediates of pigment production to leak from melanosomes, causing iris disease and ..
  31. pmc Rab27a: A key to melanosome transport in human melanocytes
    P Bahadoran
    Unité de Recherches sur la Biologie et la Physiopathologie de la Peau, Institut National de la Sante et de la Recherche Medicale U385, Faculte de Medecine, 06107 Nice Cedex 2, France
    J Cell Biol 152:843-50. 2001
    Normal pigmentation depends on the uniform distribution of melanin-containing vesicles, the melanosomes, in the epidermis...
  32. ncbi Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle
    X Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Sci 114:1091-100. 2001
    The peripheral accumulation of melanosomes characteristic of wild-type mouse melanocytes is driven by a cooperative process involving long-range, bidirectional, microtubule-dependent movements coupled to capture and local movement in the ..
  33. ncbi Insights into melanosomes and melanin from some interesting spatial and temporal properties
    John D Simon
    Department of Chemistry, Duke University, Durham, NC, USA
    J Phys Chem B 112:13201-17. 2008
    b>Melanosomes are organelles found in a wide variety of tissues throughout the animal kingdom and exhibit a range of different shapes: spheres of up to approximately 1 mum diameters and ellipsoids with lengths of up to approximately 2 mum ..
  34. ncbi Characterization of melanosomes in murine Hermansky-Pudlak syndrome: mechanisms of hypopigmentation
    Thuyen Nguyen
    Department of Dermatology, Veterans Affairs Medical Center, University of California, San Francisco, California 94121, USA
    J Invest Dermatol 122:452-60. 2004
    ..The ashen strain only has a defect in secretion, as indicated by retention of melanosomes in melanocytes...
  35. pmc Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic
    BRENDA WATT
    Department of Pathology and Laboratory Medicine and Department of Physiology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America
    PLoS Genet 7:e1002286. 2011
    ..We speculate that PMEL mutations can model the conversion between physiological and pathological amyloid...
  36. ncbi How are proliferation and differentiation of melanocytes regulated?
    Tomohisa Hirobe
    Radiation Effect Mechanisms Research Group, National Institute of Radiological Sciences, Anagawa, Inage Ku, Chiba, Japan Graduate School of Science, Chiba University, Yayoi cho, Inage Ku, Chiba, Japan
    Pigment Cell Melanoma Res 24:462-78. 2011
    ..Melanin is synthesized in melanocytes and accumulates in special organelles, melanosomes, which upon maturation are transferred to keratinocytes...
  37. pmc Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding
    Abul K Tarafder
    Molecular Medicine, National Heart and Lung Institute NHLI, Imperial College London, London SW7 2AZ, UK
    Traffic 12:1056-66. 2011
    ..Here, we address these issues using Rab27a targeting to melanosomes as a model system. Rab27a regulates motility of lysosome-related organelles and secretory granules...
  38. pmc The role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells
    Clare E Futter
    Division of Cell Biology, Institute of Ophthalmology, University College London, London EC1V 9EL, United Kingdom
    Mol Biol Cell 15:2264-75. 2004
    b>Melanosomes within the retinal pigment epithelium (RPE) of mammals have long been thought to exhibit no movement in response to light, unlike fish and amphibian RPE...
  39. pmc Glycoprotein nonmetastatic melanoma protein b, a melanocytic cell marker, is a melanosome-specific and proteolytically released protein
    Toshihiko Hoashi
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    FASEB J 24:1616-29. 2010
    b>Melanosomes are organelles specialized for the production of melanin pigment and are specifically produced by melanocytic cells...
  40. pmc Melanophilin, the product of the leaden locus, is required for targeting of myosin-Va to melanosomes
    D William Provance
    McLaughlin Research Institute, 1520 23rd St South, Great Falls, MT 59405, USA
    Traffic 3:124-32. 2002
    ..The localization of rab27a to melanosomes did not require the function of either myosin-Va or melanophilin, but leaden function was required for the ..
  41. pmc Dynamic analyses reveal cytoprotection by RPE melanosomes against non-photic stress
    Janice M Burke
    Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI 53226 4812, USA
    Mol Vis 17:2864-77. 2011
    Isolated melanosomes are known to have antioxidant properties but whether the granules perform an antioxidant function within cells is unclear...
  42. ncbi Filopodia are conduits for melanosome transfer to keratinocytes
    Glynis Scott
    Department of Dermatology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
    J Cell Sci 115:1441-51. 2002
    b>Melanosomes are specialized melanin-synthesizing organelles critical for photoprotection in the skin...
  43. ncbi Melanosome transfer to and translocation in the keratinocyte
    Raymond E Boissy
    Department of Dermatology and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0592, USA
    Exp Dermatol 12:5-12. 2003
    ..from the melanocytes to the neighboring keratinocytes and (2) distribution and degradation of the transferred melanosomes by the recipient keratinocytes...
  44. ncbi Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes
    Norihiko Ohbayashi
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Cell Sci 125:1508-18. 2012
    ..These findings reveal a new mechanism by which the dynein-dynactin motor complex recognizes Mreg on mature melanosomes through interaction with RILP and is involved in the centripetal movement of melanosomes.
  45. ncbi The relationship between Na(+)/H(+) exchanger expression and tyrosinase activity in human melanocytes
    Dustin R Smith
    Department of Biochemistry and Molecular Biology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Exp Cell Res 298:521-34. 2004
    ..the vesicular proton pump, vesicular ATPase (V-ATPase), was found to be present in both White and Black melanosomes, indicating that organelles from both racial skin types are capable of being acidified...
  46. ncbi Trace metals as biomarkers for eumelanin pigment in the fossil record
    R A Wogelius
    School of Earth, Atmospheric, and Environmental Sciences, University of Manchester, Manchester M13 9PL, UK
    Science 333:1622-6. 2011
    ..studies have reconstructed color on the basis of melanosome structure; however, the chemistry of these proposed melanosomes has remained unknown...
  47. ncbi Neurofibromatosis type 1 protein and amyloid precursor protein interact in normal human melanocytes and colocalize with melanosomes
    Sofie De Schepper
    Department of Dermatology, Ghent University Hospital, Ghent, Belgium
    J Invest Dermatol 126:653-9. 2006
    ..In addition, we observed a colocalization of amyloid precursor protein and neurofibromin with melanosomes. Amyloid precursor protein has been proposed to function as a vesicle cargo receptor for the motor protein ..
  48. ncbi Inhibition of melanosome transfer from melanocytes to keratinocytes by lectins and neoglycoproteins in an in vitro model system
    L Minwalla
    Department of Dermatology, University of Cincinnati, Ohio 45267-0592, USA
    Pigment Cell Res 14:185-94. 2001
    We propose that some of the critical molecules involved in the transfer of melanosomes from melanocytes to keratinocytes include plasma membrane lectins and their glycoconjugates...
  49. ncbi The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking
    Kimberley A Beaumont
    Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072 QLD, Australia
    Traffic 12:627-43. 2011
    ..Rabs involved in these processes revealed the localization of GFP-Rab17 on recycling endosomes (REs) and melanosomes in melanocytic cells...
  50. pmc Gpnmb is a melanosome-associated glycoprotein that contributes to melanocyte/keratinocyte adhesion in a RGD-dependent fashion
    Mizuki Tomihari
    Department of Dermatology, The University of Texas Southwestern Medical Center and Dermatology Section Medical Service, Dallas Veterans Affairs Medical Center, Dallas, TX 75390 9069, USA
    Exp Dermatol 18:586-95. 2009
    ..These features led us to posit that Gpnmb is associated with melanosomes and involved in cell adhesion...
  51. pmc Proprotein convertases process Pmel17 during secretion
    Ralf M Leonhardt
    Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06519, USA
    J Biol Chem 286:9321-37. 2011
    Pmel17 is a melanocyte/melanoma-specific protein that traffics to melanosomes where it forms a fibrillar matrix on which melanin gets deposited...
  52. ncbi Comparison of structural and chemical properties of black and red human hair melanosomes
    Yan Liu
    Department of Chemistry, Duke University, Durham, NC 27708, USA
    Photochem Photobiol 81:135-44. 2005
    b>Melanosomes in black and red human hair are isolated and characterized by various chemical and physical techniques...
  53. ncbi Oxidative stress in ARPE-19 cultures: do melanosomes confer cytoprotection?
    Mariusz Zareba
    Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, WI 53226 4812, USA
    Free Radic Biol Med 40:87-100. 2006
    ..To determine whether melanin confers cytoprotection on RPE cells, melanosomes or control particles were introduced by phagocytosis into the human cell line ARPE-19 and oxidative stress was ..
  54. pmc The repeat domain of the melanosome fibril protein Pmel17 forms the amyloid core promoting melanin synthesis
    Ryan P McGlinchey
    Laboratorie of Biochemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0830, USA
    Proc Natl Acad Sci U S A 106:13731-6. 2009
    Pmel17 is a melanocyte protein necessary for eumelanin deposition 1 in mammals and found in melanosomes in a filamentous form. The luminal part of human Pmel17 includes a region (RPT) with 10 copies of a partial repeat sequence, pt.e...
  55. pmc Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17
    Alexander C Theos
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 17:3598-612. 2006
    ..protein that participates in the formation of the intralumenal fibrils upon which melanins are deposited in melanosomes. The Pmel17 cytoplasmic domain is truncated by the mouse silver mutation, which is associated with coat ..
  56. pmc Functional amyloid formation within mammalian tissue
    Douglas M Fowler
    Department of Chemistry, The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California, USA
    PLoS Biol 4:e6. 2006
    ....
  57. pmc Rab27a is an essential component of melanosome receptor for myosin Va
    Xufeng Wu
    Laboratories of Cell Biology and Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Biol Cell 13:1735-49. 2002
    ..Similarly, the ability of full-length myosin Va to colocalize with melanosomes and to rescue their distribution in dilute melanocytes requires exon F but not exon D...
  58. ncbi The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes
    Alistair N Hume
    Cell and Molecular Biology, Division of Biomedical Sciences, Faculty of Medicine, Imperial College, London SW7 2AZ, UK
    Traffic 3:193-202. 2002
    The function of lysosome-related organelles such as melanosomes in melanocytes, and lytic granules in cytotoxic T lymphocytes is disrupted in Griscelli syndrome and related diseases...
  59. pmc Mutation of melanosome protein RAB38 in chocolate mice
    Stacie K Loftus
    Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4471-6. 2002
    ..These observations, and the fact that green fluorescent protein-tagged RAB38 colocalizes with end-stage melanosomes in wild-type melanocytes, suggest that RAB38 plays a role in the sorting of TYRP1...
  60. ncbi Microphthalmia-associated transcription factor regulates RAB27A gene expression and controls melanosome transport
    Christine Chiaverini
    INSERM U895, Biologie et Pathologies des Cellules Mélanocytaires de la Pigmentation Cutanée au Mélanome 28, Avenue de Valombrose, 06107 Nice Cedex 2, France
    J Biol Chem 283:12635-42. 2008
    b>Melanosomes are lysosome-related organelles specialized in melanin synthesis and transport...
  61. ncbi Griscelli syndrome: a model system to study vesicular trafficking
    Mireille Van Gele
    Department of Dermatology, Ghent University Hospital, Ghent, Belgium
    Pigment Cell Melanoma Res 22:268-82. 2009
    ..Finally, we discuss how the gathered knowledge about the RAB27A-MLPH-MYO5A tripartite complex can be translated into a possible therapeutic application to reduce (hyper)pigmentation of the skin...
  62. ncbi Effects of photodegradation on the physical and antioxidant properties of melanosomes isolated from retinal pigment epithelium
    Mariusz Zareba
    Department of Ophthalmology, Medical College of Wisconsin, Milwaukee, USA
    Photochem Photobiol 82:1024-9. 2006
    b>Melanosomes of the retinal pigment epithelium (RPE) are relatively long-lived organelles that are theoretically susceptible to changes induced by exposure to visible light...
  63. ncbi A mutation in the silver gene leads to defects in melanosome biogenesis and alterations in the visual system in the zebrafish mutant fading vision
    Helia B Schonthaler
    Swiss Federal Institute of Technology, Department of Biology, and Brain Research Institute of the University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Dev Biol 284:421-36. 2005
    ..The Silver protein is required for intralumenal fibril formation in melanosomes by amylogenic cleavage...
  64. pmc Structural coloration in a fossil feather
    Jakob Vinther
    Department of Geology and Geophysics, Yale University, New Haven, CT 06520, USA
    Biol Lett 6:128-31. 2010
    ..Middle Eocene Messel Oil Shale near Darmstadt, Germany shows that they are preserved as arrays of fossilized melanosomes, the surrounding beta-keratin having degraded...
  65. pmc Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors
    Valeria Levi
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 90:318-27. 2006
    Xenopus melanophores have pigment organelles or melanosomes which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region...
  66. ncbi Fossil evidence for evolution of the shape and color of penguin feathers
    Julia A Clarke
    Department of Geological Sciences, University of Texas at Austin, Austin, TX 78712, USA
    Science 330:954-7. 2010
    ..Analyses of fossilized color-imparting melanosomes reveal that their dimensions were similar to those of non-penguin avian taxa and that the feathering may have ..
  67. ncbi Regulation of bidirectional melanosome transport by organelle bound MAP kinase
    Sean W Deacon
    Department of Cell and Structural Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Curr Biol 15:459-63. 2005
    ..In Xenopus melanophores, transport of melanosomes toward the cell center is triggered by melatonin, whereas their dispersion throughout the cytoplasm is ..
  68. pmc Rab8 regulates the actin-based movement of melanosomes
    Marion L Chabrillat
    Unité de Morphogenèse et Signalisation Cellulaires, Institut Curie, Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, Paris Cedex, France
    Mol Biol Cell 16:1640-50. 2005
    ..We devised an in vitro motility assay reconstituting the movement of melanosomes on actin bundles in the presence of ATP to investigate the role of Rab proteins in the actin-dependent movement ..
  69. pmc Pmel17 initiates premelanosome morphogenesis within multivesicular bodies
    J F Berson
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Biol Cell 12:3451-64. 2001
    b>Melanosomes are tissue-specific organelles within which melanin is synthesized and stored...
  70. pmc Griscelli syndrome restricted to hypopigmentation results from a melanophilin defect (GS3) or a MYO5A F-exon deletion (GS1)
    Gael Menasche
    Unité de Recherhe sur le Développement Normal et Pathologique du Systéme Immunitaire, Hopital Necker Enfants Malades, Paris, France
    J Clin Invest 112:450-6. 2003
    ..This spectrum of GS conditions pinpoints the distinct molecular pathways used by melanocytes, neurons, and immune cells in secretory granule exocytosis, which in part remain to be unraveled...
  71. ncbi Photobleaching of retinal pigment epithelium melanosomes reduces their ability to inhibit iron-induced peroxidation of lipids
    Andrzej Zadlo
    Department of Biophysics, Jagiellonian University, Krakow, Poland
    Pigment Cell Res 20:52-60. 2007
    ..However, unlike in skin, melanosomes in the RPE are rather long-lived organelles, which increases their risk of modifications resulting from ..
  72. ncbi Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation
    Taketsugu Tadokoro
    Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Invest Dermatol 124:1326-32. 2005
    ..These results provide a basis for understanding the origin of different skin colors and responses to UV within different races...
  73. ncbi Fossilized melanosomes and the colour of Cretaceous dinosaurs and birds
    Fucheng Zhang
    Key Laboratory of Evolutionary Systematics of Vertebrates, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, PO Box 643, Beijing 100044, China
    Nature 463:1075-8. 2010
    ..Here we report that melanosomes (colour-bearing organelles) are not only preserved in the pennaceous feathers of early birds, but also in an ..
  74. ncbi Human iridal stroma melanosomes of varying pheomelanin contents possess a common eumelanic outer surface
    Dana N Peles
    Department of Chemistry, Duke University, Durham, North Carolina 27708, USA
    J Phys Chem B 113:11346-51. 2009
    Uveal melanosomes originating in the iridal stroma contain both black (eumelanin) and red (pheomelanin) pigment...
  75. pmc Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes
    Anand Sitaram
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 23:3178-92. 2012
    Cell types that generate unique lysosome-related organelles (LROs), such as melanosomes in melanocytes, populate nascent LROs with cargoes that are diverted from endosomes...
  76. ncbi Molecular preservation of the pigment melanin in fossil melanosomes
    Johan Lindgren
    Department of Geology, Lund University, 223 62 Lund, Sweden
    Nat Commun 3:824. 2012
    ..regarded as the remains of biofilms of keratinophilic bacteria, but recently they have been reinterpreted as melanosomes; that is, colour-bearing organelles...
  77. ncbi Interactions of human Myosin Va isoforms, endogenously expressed in human melanocytes, are tightly regulated by the tail domain
    Wendy Westbroek
    Department of Dermatology, Ghent University Hospital, B Gent, Belgium
    J Invest Dermatol 120:465-75. 2003
    ..These results indicate that the myosin Va medial tail domain provides the globular tail domain with organelle-interacting specificity...
  78. ncbi The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition
    Francesca Giordano
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Hum Mol Genet 18:4530-45. 2009
    ..of the ocular albinism type 1 gene, encodes a pigment-cell-specific GPCR that localizes intracellularly to melanosomes. OA1 mutations result in ocular albinism due to alterations in melanosome formation, suggesting that OA1 is a ..
  79. pmc Rab27a regulates the peripheral distribution of melanosomes in melanocytes
    A N Hume
    Cell and Molecular Biology Section, Division of Biomedical Sciences, London, United Kingdom
    J Cell Biol 152:795-808. 2001
    ..In pigmented cells, we observed that Rab27a decorates melanosomes, whereas in nonpigmented cells Rab27a colocalizes with melanosome-resident proteins...
  80. pmc An unconventional dileucine-based motif and a novel cytosolic motif are required for the lysosomal and melanosomal targeting of OA1
    Rosanna Piccirillo
    San Raffalele Scientific Institute, DIBIT, Via Olgettina 58, 20132 Milan, Italy
    J Cell Sci 119:2003-14. 2006
    ..displaying features of G-protein-coupled receptors, yet exclusively localized to late endosomes, lysosomes and melanosomes. To dissect the signals responsible for the intracellular localization of OA1, we generated chimeric proteins ..
  81. ncbi Ultrastructural characterization of melanosomes of the human pathogenic fungus Fonsecaea pedrosoi
    Anderson J Franzen
    Laboratório de Biologia Celular de Fungos, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    J Struct Biol 162:75-84. 2008
    ..pedrosoi, the major etiologic agent of chromoblastomycosis, melanin is stored in intracellular vesicles, named melanosomes. This paper details the ultrastructural aspects of melanin formation, its storage and transportation to the ..
  82. pmc Spectral analysis by XANES reveals that GPNMB influences the chemical composition of intact melanosomes
    Tamas Haraszti
    Institute of Physical Chemistry, University of Heidelberg, Heidelberg, Germany
    Pigment Cell Melanoma Res 24:187-96. 2011
    ..has not been associated with any forms of albinism, and it is unclear whether GPNMB has any direct influence on melanosomes. Here, melanosomes from congenic strains of C57BL/6J mice mutant for Gpnmb are compared to strain-matched ..
  83. pmc Specific interaction of Gαi3 with the Oa1 G-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium
    Alejandra Young
    Jules Stein Eye Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 6:e24376. 2011
    Ocular albinism type 1, an X-linked disease characterized by the presence of enlarged melanosomes in the retinal pigment epithelium (RPE) and abnormal crossing of axons at the optic chiasm, is caused by mutations in the OA1 gene...
  84. ncbi Cyclic AMP promotes a peripheral distribution of melanosomes and stimulates melanophilin/Slac2-a and actin association
    Thierry Passeron
    INSERM U597, Biologie et Pathologie des Celllules Mélanocytaires, Faculte de Medecine, Nice, France
    FASEB J 18:989-91. 2004
    b>Melanosomes are melanin-containing organelles that belong to a recently individualized group of lysosome-related organelles...
  85. ncbi The differing embryonic origins of retinal and uveal (iris/ciliary body and choroid) melanosomes are mirrored by their phospholipid composition
    Weslyn C Ward
    Department of Chemistry, Duke University, Durham, NC, USA
    Pigment Cell Res 20:61-9. 2007
    The phospholipids present in uveal (iris/ciliary body and choroid) and retinal bovine ocular melanosomes were identified using mass spectrometry...
  86. ncbi Role of Rab family GTPases and their effectors in melanosomal logistics
    Norihiko Ohbayashi
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Biochem 151:343-51. 2012
    ..In this article, we review current knowledge regarding melanosomal logistics, with particular focus on the roles of Rab small GTPases and their effectors...
  87. pmc BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles
    Jarred J Bultema
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 287:19550-63. 2012
    ..Additionally, analysis of tyrosinase-related protein-2 and total melanin production indicates that Rab32 has unique functions that cannot be carried out by Rab38 in melanosome biogenesis...
  88. pmc Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis
    Joanne F Berson
    Dept of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6082, USA
    J Cell Biol 161:521-33. 2003
    ..The molecular mechanisms governing the formation of their unique structural features are not known. Melanosomes and their precursors are lysosome-related organelles that are characterized morphologically by intralumenal ..
  89. ncbi The Hermansky-Pudlak syndrome 3 (cocoa) protein is a component of the biogenesis of lysosome-related organelles complex-2 (BLOC-2)
    Rashi Gautam
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    J Biol Chem 279:12935-42. 2004
    ..Interaction of the Hps5 and Hps6 proteins within BLOC-2 is abolished by the three-amino acid deletion in the Hps6(ru) mutant allele, indicating that these three amino acids are important for normal BLOC-2 complex formation...
  90. ncbi Mitf contributes to melanosome distribution and melanophore dendricity
    Akiha Kawasaki
    Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Pigment Cell Melanoma Res 21:56-62. 2008
    ..In vitro, lower levels of Mitf activity induced less dendritic melanophores with aggregated melanosomes, whereas melanophores overexpressing Mitf had an extensive dendritic morphology with dispersed melanosomes...
  91. pmc ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane
    Steven T Truschel
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Traffic 10:1318-36. 2009
    b>Melanosomes are lysosome-related organelles that coexist with lysosomes within melanocytes. The pathways by which melanosomal proteins are diverted from endocytic organelles toward melanosomes are incompletely defined...
  92. pmc The mouse organellar biogenesis mutant buff results from a mutation in Vps33a, a homologue of yeast vps33 and Drosophila carnation
    Tamio Suzuki
    Human Medical Genetics Program, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Proc Natl Acad Sci U S A 100:1146-50. 2003
    ..prolonged bleeding, and immunodeficiency, the result of defective biogenesis of cytoplasmic organelles: melanosomes, lysosomes, and various storage granules...
  93. ncbi Rab27b association with melanosomes: dominant negative mutants disrupt melanosomal movement
    Yanru Chen
    Department of Cell Biology, New York University School of Medicine, New York, New York 10016, USA
    J Invest Dermatol 118:933-40. 2002
    The movement of melanosomes from post-Golgi compartments to the periphery of melanocytes is known to be regulated by factors including myosin Va and at least one Rab protein, Rab27a...
  94. ncbi Melanin structure and the potential functions of uveal melanosomes
    Lian Hong
    Pigment Cell Res 19:465-6. 2006
  95. pmc Structure-function analysis of VPS9-ankyrin-repeat protein (Varp) in the trafficking of tyrosinase-related protein 1 in melanocytes
    Kanako Tamura
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    J Biol Chem 286:7507-21. 2011
    ..implicated in the regulation of trafficking of a melanogenic enzyme tyrosinase-related protein 1 (Tyrp1) to melanosomes. However, the functional interaction between Rab32/38 and Varp and the involvement of the VPS9 domain (i.e...
  96. pmc The gene for the muted (mu) mouse, a model for Hermansky-Pudlak syndrome, defines a novel protein which regulates vesicle trafficking
    Qing Zhang
    Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Hum Mol Genet 11:697-706. 2002
    ..The mu gene regulates the synthesis of specialized mammalian organelles such as melanosomes, platelet dense granules and lysosomes...
  97. pmc Darkness descends with two Rabs
    Michael S Marks
    University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Biol 175:199-200. 2006
    ..Wasmeier et al. (this issue, p. 271) reveal a redundant role for two tissue-specific Rab proteins in regulating transport to a tissue-specific lysosome-related organelle, the melanosome...
  98. pmc Different approaches for assaying melanosome transfer
    Werner Berens
    Pigment Cell Biology Section, Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Pigment Cell Res 18:370-81. 2005
    ..In this report, we describe and summarize various novel attempts to label melanosomes in search of a reliable, specific, reproducible and quantitative assay system...
  99. ncbi The silver locus product (Silv/gp100/Pmel17) as a new tool for the analysis of melanosome transfer in human melanocyte-keratinocyte co-culture
    Suman K Singh
    Medical Biosciences Research, School of Life Sciences, University of Bradford, Bradford, West Yorkshire, UK
    Exp Dermatol 17:418-26. 2008
    b>Melanosomes are melanocyte-specific lysosome-related organelles that are transferred to keratinocytes of the epidermis and anagen hair bulb...
  100. ncbi Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake, and dispersion
    Hideya Ando
    Department of Applied Chemistry and Biotechnology, Okayama University of Science, Okayama, Japan
    J Invest Dermatol 132:1222-9. 2012
    ..Here we report that melanosomes are one example of shedding vesicle cargo, but are processed by a previously unreported mechanism...
  101. ncbi Human RPE melanosomes protect from photosensitized and iron-mediated oxidation but become pro-oxidant in the presence of iron upon photodegradation
    Bartosz Rozanowski
    Department of Genetics and Cell Biology, Institute of Biology, Pedagogical Academy of Kraków, Krakow, Poland
    Invest Ophthalmol Vis Sci 49:2838-47. 2008
    To determine the effects of human retinal pigment epithelial (RPE) cell pigment granules on photosensitized and iron ion-mediated oxidation and the effect of the photodegradation of melanosomes on their antioxidant properties.

Research Grants62

  1. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
    ..Dynein, and Myosin V, Dynamics: To study the in vivo dynamics of kinesin, dynein, and myosin V in peroxisomes, melanosomes, PC12 cells, and other cell lines, using single molecule FIONA (Fluorescence Imaging with One Nanometer ..
  2. Imaging Nonlinear Absorption of Biomarkers for Improved Detection of Melanoma
    Warren S Warren; Fiscal Year: 2010
    ..melanoma;here we dramatically improve on previous work by measuring this distribution microscopically (in melanosomes and melanocytes), at depth in tissue, and noninvasively...
  3. RPE Phenotype and Oxidative Stress
    Janice M Burke; Fiscal Year: 2013
    ..the proposed project the mechanisms underlying light-induced organelle slowing in the RPE will be probed using melanosomes as a model...
  4. BIOGENESIS OF MELANOSOMES
    Seth J Orlow; Fiscal Year: 2013
    ..These studies will greatly advance our understanding of the regulation of skin pigmentation (a major risk factor for skin cancer) and pathogenesis of pigment disorders such as OCA and vitiligo. ..
  5. Function of calcium exchangers in vetebrate pigmentation
    KEITH CHI CHENG; Fiscal Year: 2010
    ..We are testing the hypothesis that slc24a5 is a calcium exchanger located in the membranes of melanosomes or their precursors, and that the organellar ionic milieu established by its transport activity is important in ..
  6. RECEPTOR MEDIATED ENDOCYTOSIS IN ALVEOLAR MACROPHAGES
    Diane McVey Ward; Fiscal Year: 2012
    ..This autosomal recessive disease results in a phenotype that affects lysosomes, cytolytic granules, melanosomes, and platelet dense granules...
  7. Hermansky Pudlak Syndrome &melanosome formation
    GRACA GRACA RAPOSO; Fiscal Year: 2012
    b>Melanosomes are tissue-specific intracellular organelles that function in the biosynthesis, storage, and transport of melanins in pigment cells of the eye and skin melanocytes...
  8. Functional Amyloid in Melansome Biogenesis
    Michael S Marks; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Melanosomes are subcellular organelles in which melanin pigments are synthesized and stored within skin melanocytes and pigment cells of the eye...
  9. Acquisition of a Cryo-Electron Microscope
    Susan Hafenstein; Fiscal Year: 2012
    ..Using tomography the number of melanosomes will be quantified and the 3D structure refined in mutant and wild type zebrafish...
  10. Biology of Fungal Melanin
    Arturo Casadevall; Fiscal Year: 2013
    ..neoformans is vesicle-associated;2) To establish the relationship between extracellular vesicles, melanosomes, and virulence;3) To investigate the molecular events of C. neoformans melanin biosynthesis...
  11. Microspectroscopy of aging effects in RPE melanosomes
    Vladislav V Yakovlev; Fiscal Year: 2010
    ..This is a new research proposal (R15), which aims at understanding the role of melanin containing melanosomes in RPE cells...
  12. Phosphoinositide Signaling Regulates Melanogenesis
    Anand K Ganesan; Fiscal Year: 2013
    ..to the melanosome;2) identify cellular signals that direct transport vesicles t fuse with autophagosomes or melanosomes;3) determine how cells coordinate melanosome maturation with the transcription of enzymes that produce melanin ..
  13. RPE Cell Biology of Myosin VIIa
    David S Williams; Fiscal Year: 2013
    ..findings and developments by: (1) Studying the roles of myosin VIIa and microtubule motors in the transport of melanosomes, phagosomes, and lysosomes in the RPE;(2) Identifying and testing the function of microtubule-binding domain(s) ..
  14. Signal Transduction Mechanisms in Ocular Albinism
    Vinit Mahajan; Fiscal Year: 2005
    ..Expressed by melanosomes within the retinal pigment epithelium, OA1 amino acid sequence analysis and binding studies suggest it is a ..
  15. The pigmentary system: securing a place in the sun
    Andrzej Slominski; Fiscal Year: 2009
    ..and diverse topics of public health interest, ranging from developmental biology, biochemistry of pigmentation, melanosomes and immune recognition, stem cells and malignant transformation, to the environmental effects on pigmentation ..
  16. Role of Melanocyte Defect in Smyth Line Vitiligo
    Gisela Erf; Fiscal Year: 2001
    ..to visible signs of vitiligo, the earliest abnormality detected within SL melanocytes are irregularly shaped melanosomes containing pigmented membrane extensions, hyperactive melanization, and selective autophagocytosis of ..
  17. Pigmentation-dependent melanosomal disease
    MICHAEL GARY ANDERSON; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Melanosomes are a specialized organelle of melanocytes that exist in order to conduct a highly specialized task, producing and storing melanin pigment...
  18. Pathfinding of Ganglion Cell Axons and Ocular Albinism
    Debora Farber; Fiscal Year: 2002
    ..It encodes a G-protein coupled-receptor of unknown function that is localized on the membrane of melanosomes. Melanogenesis occurs in these organelles and tyrosinase is the key enzyme involved in this process...
  19. Molecular Analyses of Hermansky-Pudlak Syndrome Genes
    RICHARD SWANK; Fiscal Year: 2005
    ..action of genes responsible for the normal biosynthesis of specialized mammalian subcellular organelles such as melanosomes, platelet dense granules and lysosomes...
  20. ANIMAL MODELS IN INHERITED HEMORRHAGIC DISEASES
    RICHARD SWANK; Fiscal Year: 2004
    ..visual and pigmentation deficiencies due to abnormalities in melanosomes and premature death due to fibrotic lung disease...
  21. Albinism: Defects in Tyrosinase, Amines, or Melanin
    Suzanne Roffler Tarlov; Fiscal Year: 2005
    ..in vivo using tissues from albino mice including the OA1 knockout in which a G protein coupled receptor within melanosomes is mutated...
  22. Protein-Protein Interactions in Melanosome Biogenesis
    Vijayasaradhi Setaluri; Fiscal Year: 2006
    ..Although gp75, an abundant melanosomal protein, is thought to influence biogenesis of melanosomes, specific role(s) it plays in human skin pigmentation is not known...
  23. The composition and function of BLOC-2 in melanogenesis
    Raymond Boissy; Fiscal Year: 2009
    Skin pigmentation is regulated by the synthesis of melanized melanosomes. The biogenesis of these melanosomes is an excellent model to explore the various molecular mechanisms that govern the chaperoning of gene products through the ..
  24. ORGANELLE MEMBRANES IN PLATELET STORAGE POOL DISEASE
    LUANNE PETERS; Fiscal Year: 2009
    ..recessive disorder of organelle biogenesis that affects lysosomes and the lysosome-related organelles (LROs), melanosomes and platelet dense bodies, leading to lysosomal storage disease/lung fibrosis, oculocutaneous albinism and ..
  25. OPTICS AND PHOTOTOXICITY IN HUMAN SKIN
    John Parrish; Fiscal Year: 1993
    ..Selective microvascular hemostasis will be studied and optimized. Selective photothermolysis of melanosomes, and studies of pigment cell damage mechanisms and repair in humans will be conducted...
  26. Actin and myosins in organelle motility
    Christina King Smith; Fiscal Year: 2003
    ..of the proposed research are to characterize the role of actin filaments and myosin motors in transport of melanosomes (pigment granules) within retinal pigment epithelial (RPE) cells from fish...
  27. INHERITED DISEASES AFFECTING PLATELET FUNCTION
    RICHARD SWANK; Fiscal Year: 2004
    ..regulate the synthesis and trafficking of subcellular organelles such as platelet dense granules, lysosomes and melanosomes. The Specific Aims of the proposal are to: 1) complete the identification and partial characterization of the ..
  28. Structural biology of BEACH-containing proteins
    Liang Tong; Fiscal Year: 2006
    ..elegans F10F2.1. The CHS protein may be involved in the trafficking of lysosomes, melanosomes and other vesicles...
  29. Genetic Analysis of Vesicle Trafficking Pathways
    Lisa Webb; Fiscal Year: 2003
    ..to the biogenesis and function of three developmentally related cellular organelles (platelet dense bodies, melanosomes, and lysosomes) and is involved in the pathogenesis of HPS...
  30. PanAmerican Society for Pigment Cell Research (PASPCR)
    Frank Meyskens; Fiscal Year: 2004
    ..Cell Diseases; 2) Mechanisms of Melanocyte Proliferations and Differentiation; 3) The Biology of Melanin and Melanosomes and Its Potential for Disease; 4) Newer Animal Models for Pigmentary Abnormalities and Melanoma; 5) Non-..
  31. Role of Prostaglandins & Phospholipase A in melanocytes
    Glynis Scott; Fiscal Year: 2009
    ..We hypothesize that PG and phospholipases stimulate HM dendricity, MS transfer &pigmentation through different mechanisms that act synergistically to modulate cutaneous pigmentation following UVR. ..
  32. Molecular Basis for Platelet Dense Granule Biogenesis
    ESTEBAN DELL ANGELICA; Fiscal Year: 2004
    ....
  33. MELANOSOME PROTEIN SORTING, FOLDING & ANTIGEN PROCESSING
    Michael Marks; Fiscal Year: 2002
    ..The mechanisms by which these proteins are sorted to melanosomes and the relationship between melanosomal and MHC class II antigen sorting pathways are poorly understood...
  34. BIOLOGICAL PROPERTIES OF RPE MELANIN
    Janice M Burke; Fiscal Year: 2010
    ..Additionally, RPE melanosomes are long-live organelles that could undergo aging changes that alter their redox function...
  35. Role of BLOC-3 in Lysosome and Melanosome Biogenesis
    ESTEBAN DELL ANGELICA; Fiscal Year: 2007
    b>Melanosomes are cell-type-specific, membrane-bounded organelles in which melanin pigments are synthesized and stored...
  36. Determinants of Phenotype in Retinal Pigment Epithelium
    Janice Burke; Fiscal Year: 2007
    ..To determine whether the anticipated loss of polarity in RPE cells leads to a clinically or morphologically detectable retinal degeneration. ..
  37. Iron and copper transporter trafficking in healthy and diseased melanocytes
    Michael Marks; Fiscal Year: 2007
    ..Preliminary data suggest that an ATP7A cohort in melanocytes localizes to melanosomes dependent on its target cuproenzyme, tyrosinase, and on BLOC-1, a cytoplasmic complex defective in the ..
  38. Nmb and melanosome function in eye pigment cells
    Michael Marks; Fiscal Year: 2005
    ..Several indirect lines of evidence suggest that Nmb plays a role in the structure and/or function of melanosomes, the organelles in which melanins are synthesized and stored...
  39. Dissecting the Molecular Basis of Ocular Albinism type 1
    Maria Vittoria Schiaffino; Fiscal Year: 2007
    ..by severe reduction of visual acuity, retinal hypopigmentation, foveal hypoplasia, and the presence of giant melanosomes (macromelanosomes) in skin melanocytes and retinal pigment epithelium...
  40. STUDIES OF HUMAN PIGMENTATION DISORDERS
    Richard Spritz; Fiscal Year: 2005
    ....
  41. PHENOLS/CATECHOLS IN OCCUPATIONAL/CONTACT VITILIGO SKIN
    Raymond Boissy; Fiscal Year: 2002
    ....
  42. Missing Mutations in Oculocutaneous and Ocular Albinism
    Richard Spritz; Fiscal Year: 2006
    ..Together, these studies should provide a greatly improved understanding of the molecular pathogenesis of oculocutaneous and ocular albinism. [unreadable] [unreadable]..
  43. GENE DISCOVERY FOR CRANIOFACIAL DISORDERS
    Richard Spritz; Fiscal Year: 2007
    ..We will apply state-of-art bioinformatics tools to analyze and interpret the data, ali of which we will deposit in appropriate public data repositories. ..
  44. ON THE ROLE OF THE HPS GENE PRODUCT IN MELANOCYTES
    Raymond Boissy; Fiscal Year: 2002
    ..These studies will contribute to our knowledge of cellular trafficking in general and the pathophysiology of HPS in specific. ..
  45. PROLIFERATION & MALIGNANT TRANSFORMATION OF MELANOCYTES
    Ruth Halaban; Fiscal Year: 2004
    ..Our functional studies may provide a basis for the design of new therapeutical agents for the treatment of patients with metastatic melanomas. ..
  46. 2003 Conference Pan-American Society Pigment Cell Resea
    Ruth Halaban; Fiscal Year: 2003
    ..3. To provide partial support to students and junior faculty to attend the meeting. They request funds for the 2003 meeting as well as for the 2 subsequent PASPCR annual meetings in years 2004 and 2006. ..
  47. GENETIC STUDIES OF MARGARITA ISLAND ECTODERMAL DYSPLASIA
    Richard Spritz; Fiscal Year: 2002
    ..The last aim is to initiate studies to determine the function of the ED4 gene. ..
  48. 2004 National Vitiligo Foundation Scientific Conference
    Raymond Boissy; Fiscal Year: 2004
    ..In addition, mini-symposiums will be organized for each of the sessions. ..
  49. Epigenetic Chromatin Changes as Melanoma Markers
    Ruth Halaban; Fiscal Year: 2006
    ..Altogether, the results are likely to generate a novel marker(s) that can be used to assess propensity for malignant transformation, tumor progression and/or sensitivity to chemotherapeutic drugs that target chromatin conformation. ..
  50. ETHANOL, RETINOIDS, AND CONGENITAL HEART MALFORMATIONS
    Janee Gelineau van Waes; Fiscal Year: 2003
    ..abstract_text> ..
  51. Pigmentation and Diversity Conference
    ISABELLE LE POOLE; Fiscal Year: 2007
    ..unique facets and diverse topics of a public health interest that relate to the biochemistry of pigmentation, melanosomes and immune recognition, stem cells and malignant transformation, gene expression and neural crest development, ..
  52. NEUTROPHINS AND RETINAL DEVELOPMENT
    Janet Sparrow; Fiscal Year: 2001
    ..The use of neurotrophins in conjunction with gene therapy or transplantation may also have potential for reversing retinal degenerative changes and lead to the restoration of vision. ..
  53. The function of proteins associated with albinism
    MURRAY BRILLIANT; Fiscal Year: 2005
    ..The characterization of mouse models for these hypopigmentation disorders will provide a system to test the efficacy of genetic and biochemical intervention in the treatment of the homologous human disorders. ..
  54. GENETIC AND MOLECULAR ANALYSES OF MUTATIONS
    MURRAY BRILLIANT; Fiscal Year: 2002
    ..In addition, differential display, cDNA subtraction, and/or gene expression profiling will be used to compare mutant and non-mutant tissues in an attempt to identify Sox6 targets. ..
  55. Characterization of nuclear proteome in normal and diseased liver
    Svetlana Lutsenko; Fiscal Year: 2007
    ..The proteomic information and methodology generated during this study will be applicable for analysis of other hepatic disorders in humans. [unreadable] [unreadable] [unreadable]..
  56. Chemopreventive treatment of familial melanoma
    ISABELLE LE POOLE; Fiscal Year: 2008
    ..3] To quantify melanoma tumor growth and measure immunologic parameters (cytokine secretion by ELISA and ELISPOT) in mice treated with bleaching agents and IL-2. [unreadable] [unreadable] [unreadable]..
  57. A Multidisciplinary Analysis of Gelsolin Amyloid Disease
    Jeffery W Kelly; Fiscal Year: 2010
    ..The elucidation of the FAF pathogenic cascade together with the development of candidate pharmacologic agents will significantly advance our understanding of human amyloid diseases. ..
  58. Protein Misfolding Diseases
    Jeffery Kelly; Fiscal Year: 2006
    ..The integrated scientific nature of this meeting is essential to intervene in and understand these disorders representing the most costly contemporary health problem. [unreadable] [unreadable]..
  59. Caloric Restriction and Aging in Eyes
    Peter Gouras; Fiscal Year: 2010
    ..Because many of these primates are near the end of their lifespan, there is an element of urgency in our goal to correlate noninvasive clinical testing prior with post mortem histology. ..
  60. PROTEIN STORAGE IN POST GOLGI ORGANELLES
    Regina Kuliawat; Fiscal Year: 2004
    ..network is the parent organelle from which components of secretory granules in neuroendocrine cells and melanosomes in melanocytes originate...
  61. Beta-cell Granule Protein Profile by Split Reporter Ass*
    Regina Kuliawat; Fiscal Year: 2005
    ..abstract_text> ..