hela cells

Summary

Summary: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks (one of several pseudonyms). These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

Top Publications

  1. ncbi Prediction of mammalian microRNA targets
    Benjamin P Lewis
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 115:787-98. 2003
  2. pmc MicroRNA genes are transcribed by RNA polymerase II
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul, Korea
    EMBO J 23:4051-60. 2004
  3. pmc miR-148 targets human DNMT3b protein coding region
    Anja M Duursma
    Division of Tumor Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    RNA 14:872-7. 2008
  4. ncbi MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
    Jürgen Cox
    Department for Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Biotechnol 26:1367-72. 2008
  5. pmc MicroRNA targeting specificity in mammals: determinants beyond seed pairing
    Andrew Grimson
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 27:91-105. 2007
  6. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
  7. ncbi Widespread changes in protein synthesis induced by microRNAs
    Matthias Selbach
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
    Nature 455:58-63. 2008
  8. pmc The impact of microRNAs on protein output
    Daehyun Baek
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 455:64-71. 2008
  9. ncbi p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy
    Serhiy Pankiv
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 282:24131-45. 2007
  10. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003

Detail Information

Publications335 found, 100 shown here

  1. ncbi Prediction of mammalian microRNA targets
    Benjamin P Lewis
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 115:787-98. 2003
    ..The predicted regulatory targets of mammalian miRNAs were enriched for genes involved in transcriptional regulation but also encompassed an unexpectedly broad range of other functions...
  2. pmc MicroRNA genes are transcribed by RNA polymerase II
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul, Korea
    EMBO J 23:4051-60. 2004
    ..These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes...
  3. pmc miR-148 targets human DNMT3b protein coding region
    Anja M Duursma
    Division of Tumor Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    RNA 14:872-7. 2008
    ..More generally, we provide evidence that coding regions of human genes can be targeted by miRNAs, and that such a mechanism might play a role in determining the relative abundance of different splice variants...
  4. ncbi MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification
    Jürgen Cox
    Department for Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Biotechnol 26:1367-72. 2008
    ..MaxQuant automatically quantifies several hundred thousand peptides per SILAC-proteome experiment and allows statistically robust identification and quantification of >4,000 proteins in mammalian cell lysates...
  5. pmc MicroRNA targeting specificity in mammals: determinants beyond seed pairing
    Andrew Grimson
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 27:91-105. 2007
    ..Because it predicts site efficacy without recourse to evolutionary conservation, the model also identifies effective nonconserved sites and siRNA off-targets...
  6. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
    ..The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes...
  7. ncbi Widespread changes in protein synthesis induced by microRNAs
    Matthias Selbach
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, D 13125 Berlin, Germany
    Nature 455:58-63. 2008
    ..Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes...
  8. pmc The impact of microRNAs on protein output
    Daehyun Baek
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 455:64-71. 2008
    ..The impact of microRNAs on the proteome indicated that for most interactions microRNAs act as rheostats to make fine-scale adjustments to protein output...
  9. ncbi p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy
    Serhiy Pankiv
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Biol Chem 282:24131-45. 2007
    ..In fact, p62 bodies and these structures are indistinguishable. Taken together, our results clearly suggest that p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy...
  10. ncbi The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  11. ncbi Andromeda: a peptide search engine integrated into the MaxQuant environment
    Jürgen Cox
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    J Proteome Res 10:1794-805. 2011
    ..We demonstrate the flexibility of the system by implementing the capability to identify cofragmented peptides, significantly improving the total number of identified peptides...
  12. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
    ..We suggest that p62 may, via LC3, be involved in linking polyubiquitinated protein aggregates to the autophagy machinery...
  13. ncbi Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu
    Stuart J D Neil
    Aaron Diamond AIDS Research Center and Laboratory of Retrovirology, The Rockefeller University, 455 First Avenue, New York, New York 10016, USA
    Nature 451:425-30. 2008
    ..Vpu co-localized with CD317 and inhibited these effects. Inhibition of Vpu function and consequent mobilization of tetherin's antiviral activity is a potential therapeutic strategy in HIV/AIDS...
  14. ncbi Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy
    Sophie Pattingre
    Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Cell 122:927-39. 2005
    ..This antiautophagy function of Bcl-2 may help maintain autophagy at levels that are compatible with cell survival, rather than cell death...
  15. pmc Parkin is recruited selectively to impaired mitochondria and promotes their autophagy
    Derek Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 183:795-803. 2008
    ..These results show that Parkin promotes autophagy of damaged mitochondria and implicate a failure to eliminate dysfunctional mitochondria in the pathogenesis of Parkinson's disease...
  16. ncbi Switching from repression to activation: microRNAs can up-regulate translation
    Shobha Vasudevan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 318:1931-4. 2007
    ..Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle...
  17. ncbi Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
    Asako Sakaue-Sawano
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
    Cell 132:487-98. 2008
    ..These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events...
  18. ncbi XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor
    H Yoshida
    Graduate School of Biostudies, Kyoto University, Kyoto 606 8304, Japan
    Cell 107:881-91. 2001
    ..Our results reveal features of the UPR conserved during evolution and clarify the relationship between IRE1- and ATF6-dependent pathways...
  19. pmc PINK1 is selectively stabilized on impaired mitochondria to activate Parkin
    Derek P Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Biol 8:e1000298. 2010
    ..In addition, they support a novel model for the negative selection of damaged mitochondria, in which PINK1 signals mitochondrial dysfunction to Parkin, and Parkin promotes their elimination...
  20. ncbi Mdm2 promotes the rapid degradation of p53
    Y Haupt
    Lautenberg Center for General and Tumor Immunology, The Hebrew University Haddassah Medical School, Jerusalem, Israel
    Nature 387:296-9. 1997
    ..During recovery from DNA damage, maximal Mdm2 induction coincides with rapid p53 loss. We propose that the Mdm2-promoted degradation of p53 provides a new mechanism to ensure effective termination of the p53 signal...
  21. ncbi PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1
    Sven Geisler
    Laboratory of Functional Neurogenetics, Otfried Muller Strasse 27, 72076 Tubingen, Germany
    Nat Cell Biol 12:119-31. 2010
    ..Thus, our data provide functional links between PINK1, Parkin and the selective autophagy of mitochondria, which is implicated in the pathogenesis of Parkinson's disease...
  22. ncbi The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 427:848-53. 2004
    ..Our studies identify TRIM5alpha as a species-specific mediator of innate cellular resistance to HIV-1 and reveal host cell components that modulate the uncoating of a retroviral capsid...
  23. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  24. ncbi The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
    ..In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript...
  25. ncbi RAS is regulated by the let-7 microRNA family
    Steven M Johnson
    Department of Molecular, Cellular and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520, USA
    Cell 120:635-47. 2005
    ..let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer...
  26. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  27. pmc A quantitative atlas of mitotic phosphorylation
    Noah Dephoure
    Department of Cell Biology, Harvard University Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:10762-7. 2008
    ..Analysis of non-proline site-containing phosphopeptides identified two unique motifs that suggest there are at least two undiscovered mitotic kinases...
  28. pmc Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin
    Atsushi Tanaka
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 191:1367-80. 2010
    ..Inhibition of Drp1-mediated mitochondrial fission, the proteasome, or p97 prevents Parkin-induced mitophagy...
  29. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
    ..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
  30. ncbi Genome-scale RNAi screen for host factors required for HIV replication
    Honglin Zhou
    Department of Virus and Cell Biology, Merck and Co, Inc, West Point, PA 19486, USA
    Cell Host Microbe 4:495-504. 2008
    ..This study highlights both the power and shortcomings of large scale loss-of-function screens in discovering host-pathogen interactions...
  31. ncbi Dynasore, a cell-permeable inhibitor of dynamin
    Eric Macia
    Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, Inc, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Dev Cell 10:839-50. 2006
    ..Thus, dynamin acts at two steps during clathrin coat formation; GTP hydrolysis is probably needed at both steps...
  32. ncbi A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA
    G Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Science 293:834-8. 2001
    ..Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression...
  33. ncbi Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3
    Rashu B Seth
    Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Cell 122:669-82. 2005
    ..The transmembrane domain targets MAVS to the mitochondria, implicating a new role of mitochondria in innate immunity...
  34. pmc A third-generation lentivirus vector with a conditional packaging system
    T Dull
    Cell Genesys, Foster City, California 94404, USA
    J Virol 72:8463-71. 1998
    ..While the actual biosafety of the vector will ultimately be proven in vivo, the improved design presented here should facilitate testing of lentivirus vectors...
  35. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
    ..endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and ..
  36. ncbi MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation
    Ulf Andersson Ørom
    Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, DK 2200, Copenhagen, Denmark
    Mol Cell 30:460-71. 2008
    ..The results show that miR-10a may positively control global protein synthesis via the stimulation of ribosomal protein mRNA translation and ribosome biogenesis and hereby affect the ability of cells to undergo transformation...
  37. ncbi Identification of novel genes coding for small expressed RNAs
    M Lagos-Quintana
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, , Germany
    Science 294:853-8. 2001
    ..This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated...
  38. ncbi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
  39. pmc PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy
    Noriyuki Matsuda
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8506, Japan
    J Cell Biol 189:211-21. 2010
    ..Some pathogenic mutations of PINK1 and Parkin interfere with the aforementioned events, suggesting an etiological importance. These results provide crucial insight into the pathogenic mechanisms of PD...
  40. ncbi Mitofusin 2 tethers endoplasmic reticulum to mitochondria
    Olga Martins de Brito
    Dulbecco Telethon Institute, Venetian Institute of Molecular Medicine, Via Orus 2, 35129 Padova, Italy
    Nature 456:605-10. 2008
    ..Ablation or silencing of mitofusin 2 in mouse embryonic fibroblasts and HeLa cells disrupts ER morphology and loosens ER-mitochondria interactions, thereby reducing the efficiency of ..
  41. pmc STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx
    Jen Liou
    Department of Molecular Pharmacology, Stanford University Medical School, California 94305, USA
    Curr Biol 15:1235-41. 2005
    ..Our study suggests that STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(2+) store depletion to Ca(2+) influx...
  42. ncbi Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes
    Christian Matranga
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Cell 123:607-20. 2005
    ..For siRNAs, virtually all RISC is assembled through this cleavage-assisted mechanism. In contrast, passenger-strand cleavage is not important for the incorporation of miRNAs that derive from mismatched duplexes...
  43. pmc Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
    Angie M Cheng
    Ambion, Inc 2130 Woodward Street, Austin, TX 78744 1832, USA
    Nucleic Acids Res 33:1290-7. 2005
    ..We employed a library of miRNA inhibitors to screen for miRNA involved in cell growth and apoptosis. In HeLa cells, we found that inhibition of miR-95, 124, 125, 133, 134, 144, 150, 152, 187, 190, 191, 192, 193, 204, 211, 218, ..
  44. ncbi Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
    P Li
    Howard Hughes Medical Institute, and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 91:479-89. 1997
    ....
  45. ncbi Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
    Gunter Meister
    Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
    Mol Cell 15:185-97. 2004
    ..The specific role of Ago2 in guiding target RNA cleavage was confirmed independently by siRNA-based depletion of individual Ago members in combination with a sensitive positive-readout reporter assay...
  46. pmc Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
    Beate Neumann
    MitoCheck Project Group, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nature 464:721-7. 2010
    ..As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community...
  47. pmc Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
    Nancy Kedersha
    Division of Rheumatology and Immunology, Harvard Medical School, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Cell Biol 169:871-84. 2005
    ..We propose that mRNA released from disassembled polysomes is sorted and remodeled at SGs, from which selected transcripts are delivered to PBs for degradation...
  48. ncbi De-ubiquitination and ubiquitin ligase domains of A20 downregulate NF-kappaB signalling
    Ingrid E Wertz
    Department of Molecular Oncology, School of Medicine, University of California, Davis, Davis, California 95616, USA
    Nature 430:694-9. 2004
    ..We also provide an example of a protein containing separate ubiquitin ligase and DUB domains, both of which participate in mediating a distinct regulatory effect...
  49. pmc Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5' UTR as in the 3' UTR
    J Robin Lytle
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, USA
    Proc Natl Acad Sci U S A 104:9667-72. 2007
    ..In human HeLa cells, which express endogenous let-7a miRNA, the translational efficiency of these IRES-containing reporters with 5' ..
  50. pmc Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter
    Joshua M Baughman
    Department of Systems Biology, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 476:341-5. 2011
    ..Our genomic, physiological, biochemical and pharmacological data firmly establish MCU as an essential component of the mitochondrial Ca(2+) uniporter...
  51. pmc Expanding the microRNA targeting code: functional sites with centered pairing
    Chanseok Shin
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Mol Cell 38:789-802. 2010
    ..This expanded repertoire of cleavage targets and the identification of the centered site type help explain why central regions of many miRNAs are evolutionarily conserved...
  52. ncbi Molecular characterization of mitochondrial apoptosis-inducing factor
    S A Susin
    Centre National de la Recherche Scientifique, UPR 420, Villejuif, France
    Nature 397:441-6. 1999
    ..These results indicate that AIF is a mitochondrial effector of apoptotic cell death...
  53. ncbi DNA damage foci at dysfunctional telomeres
    Hiroyuki Takai
    Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, NY 10021, USA
    Curr Biol 13:1549-56. 2003
    ..Furthermore, induction of TIFs through TRF2 inhibition provides an opportunity to study the DNA damage response within the context of well-defined, physically marked lesions...
  54. ncbi Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004
    ..Reducing the expression of Ring2 results in a dramatic decrease in the level of ubiquitinated H2A in HeLa cells. Chromatin immunoprecipitation analysis demonstrated colocalization of dRing with ubiquitinated H2A at the PRE ..
  55. ncbi Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c
    X Liu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cell 86:147-57. 1996
    ..Cells undergoing apoptosis in vivo showed increased release of cytochrome c to their cytosol, suggesting that mitochondria may function in apoptosis by releasing cytochrome c...
  56. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  57. pmc Specific recognition and accelerated uncoating of retroviral capsids by the TRIM5alpha restriction factor
    Matthew Stremlau
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School Division of AIDS, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:5514-9. 2006
    ..Inhibiting the proteasome did not abrogate restriction. Thus, TRIM5alpha restricts retroviral infection by specifically recognizing the capsid and promoting its rapid, premature disassembly...
  58. ncbi Role for Rab7 in maturation of late autophagic vacuoles
    Stefanie Jäger
    Institute of Biochemistry, University of Kiel, Olshausenstr 40, 24098 Kiel, Germany
    J Cell Sci 117:4837-48. 2004
    ..In conclusion, our results showed a role for Rab7 in the final maturation of late autophagic vacuoles...
  59. pmc Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins
    Takeharu Nagai
    Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Proc Natl Acad Sci U S A 101:10554-9. 2004
    ..Our study provides an important guide for the development and improvement of indicators using GFP-based FRET...
  60. ncbi Global, in vivo, and site-specific phosphorylation dynamics in signaling networks
    Jesper V Olsen
    Center for Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK 5230 Odense, Denmark
    Cell 127:635-48. 2006
    ..6,600 phosphorylation sites on 2,244 proteins and have determined their temporal dynamics after stimulating HeLa cells with epidermal growth factor (EGF) and recorded them in the Phosida database...
  61. ncbi LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
    Yukiko Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji 38, Okazaki 444 8585, Japan
    J Cell Sci 117:2805-12. 2004
    ..Generation of the form II correlates with autophagosome association of GABARAP and GATE16. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II...
  62. ncbi The TBK1 adaptor and autophagy receptor NDP52 restricts the proliferation of ubiquitin-coated bacteria
    Teresa L M Thurston
    Medical Research Council Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Cambridge, UK
    Nat Immunol 10:1215-21. 2009
    ..We conclude that human cells utilize the ubiquitin system and NDP52 to activate autophagy against bacteria attempting to colonize their cytosol...
  63. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
    ..Remarkably, inhibition of Drp1 blocks cell death, implicating mitochondrial fission as an important step in apoptosis...
  64. ncbi Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin
    A Miyawaki
    Department of Pharmacology, University of California, San Diego, La Jolla 92093 0647, USA
    Nature 388:882-7. 1997
    ..We have visualized free Ca2+ dynamics in the cytosol, nucleus and endoplasmic reticulum of single HeLa cells transfected with complementary DNAs encoding chimaeras bearing appropriate localization signals...
  65. pmc A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter
    Diego De Stefani
    Department of Biomedical Science, University of Padua, 35121 Padua, Italy
    Nature 476:336-40. 2011
    ..Short interfering RNA (siRNA) silencing of MCU in HeLa cells markedly reduced mitochondrial Ca(2+) uptake...
  66. pmc Complexes between the LKB1 tumor suppressor, STRAD alpha/beta and MO25 alpha/beta are upstream kinases in the AMP-activated protein kinase cascade
    Simon A Hawley
    Division of Molecular Physiology, University of Dundee, Dundee DD1 5EH, UK
    J Biol 2:28. 2003
    ..We recently showed that LKB1 exists as a complex with two accessory subunits, STRAD alpha/beta and MO25 alpha/beta...
  67. ncbi Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition
    C Du
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 102:33-42. 2000
    ..Overexpression of Smac increases cells' sensitivity to apoptotic stimuli. Smac is the second mitochondrial protein, along with cytochrome c, that promotes apoptosis by activating caspases...
  68. pmc Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription
    Xiangting Wang
    Howard Hughes Medical Institute
    Nature 454:126-30. 2008
    ....
  69. ncbi RNA exosome depletion reveals transcription upstream of active human promoters
    Pascal Preker
    Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, C F Møllers Alle, Building 1130, Aarhus University, Denmark
    Science 322:1851-4. 2008
    ..We propose that PROMPT transcription is a common characteristic of RNA polymerase II (RNAPII) transcribed genes with a possible regulatory potential...
  70. ncbi HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
    Y Feng
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
    Science 272:872-7. 1996
    ..Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates...
  71. pmc Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy
    J W Miller
    Department of Molecular Genetics and Microbiology, Centers for Gene Therapy and Mammalian Genetics, University of Florida College of Medicine, Gainesville, FL 32610, USA
    EMBO J 19:4439-48. 2000
    ..We propose that DM1 disease is caused by aberrant recruitment of the EXP proteins to the DMPK transcript (CUG)(n) expansion...
  72. ncbi The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
    P H Maxwell
    Wellcome Trust Centre for Human Genetics, Oxford, UK
    Nature 399:271-5. 1999
    ..Thus, constitutive HIF-1 activation may underlie the angiogenic phenotype of VHL-associated tumours. The pVHL/HIF-1 interaction provides a new focus for understanding cellular oxygen sensing...
  73. pmc MICU1 encodes a mitochondrial EF hand protein required for Ca(2+) uptake
    Fabiana Perocchi
    Center for Human Genetic Research, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Nature 467:291-6. 2010
    ..Its discovery may lead to the complete molecular characterization of mitochondrial calcium uptake pathways, and offers genetic strategies for understanding their contribution to normal physiology and disease...
  74. pmc Cleavage of the siRNA passenger strand during RISC assembly in human cells
    Philipp J F Leuschner
    Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA, Dr Bohr Gasse 3 5, 1030 Vienna, Austria
    EMBO Rep 7:314-20. 2006
    ..Interestingly, target RNA cleavage can be rescued if an otherwise non-cleavable passenger strand shows a nick at the scissile phosphodiester bond, which further indicates that the cleavage event per se is not essential...
  75. ncbi BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly
    Tomomi Kuwana
    La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
    Mol Cell 17:525-35. 2005
    ..In this model, the simple inhibition of antiapoptotic functions is insufficient to induce apoptosis unless a direct activator of Bax or Bak is present...
  76. pmc Antagonism to and intracellular sequestration of human tetherin by the human immunodeficiency virus type 2 envelope glycoprotein
    Anna Le Tortorec
    Department of Infectious Disease, King s College London School of Medicine, Guy s Hospital, London SE1 9RT, United Kingdom
    J Virol 83:11966-78. 2009
    ..Finally, we recapitulated these observations in HIV-2-infected CD4+ T-cell lines, demonstrating that tetherin antagonism and sequestration occur at physiological levels of Env expression during virus replication...
  77. pmc Global analysis of the insulator binding protein CTCF in chromatin barrier regions reveals demarcation of active and repressive domains
    Suresh Cuddapah
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genome Res 19:24-32. 2009
    ..Our data indicate that CTCF may play important roles in the barrier activity of insulators, and this study provides a resource for further investigation of the CTCF function in organizing chromatin in the human genome...
  78. ncbi Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase
    Simon A Hawley
    Division of Molecular Physiology University of Dundee, Dundee, Scotland, United Kingdom
    Cell Metab 2:9-19. 2005
    ..Our results suggest a potential Ca(2+)-dependent neuroprotective pathway involving phosphorylation and activation of AMPK by CaMKKbeta...
  79. pmc An expanded palette of genetically encoded Ca²⁺ indicators
    Yongxin Zhao
    Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada
    Science 333:1888-91. 2011
    ..This series enables improved single-color Ca(2+) imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca(2+) was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based ..
  80. pmc HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP
    Bijan Sobhian
    Laboratoire de Virologie Moleculaire, Institut de Genetique Humaine, CNRS UPR1142, Montpellier, France
    Mol Cell 38:439-51. 2010
    ....
  81. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  82. pmc A dual pressure linear ion trap Orbitrap instrument with very high sequencing speed
    Jesper V Olsen
    Thermo Fisher Scientific Bremen GmbH, Hanna Kunath Strasse 11, 28199 Bremen, Germany
    Mol Cell Proteomics 8:2759-69. 2009
    ..Higher-collision energy dissociation with high mass accuracy Orbitrap readout is as sensitive as ion trap MS/MS scans in the previous generation of the instrument...
  83. ncbi A microRNA in a multiple-turnover RNAi enzyme complex
    Gyorgy Hutvagner
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Lazare Research Building, Room 825, 364 Plantation Street, Worcester, MA 01605, USA
    Science 297:2056-60. 2002
    ..Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells...
  84. pmc The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3
    Pascal Drané
    IGMBC Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch F 67400, France
    Genes Dev 24:1253-65. 2010
    ..Taken together, our data demonstrate that DAXX functions as a bona fide histone chaperone involved in the replication-independent deposition of H3.3...
  85. pmc HuR recruits let-7/RISC to repress c-Myc expression
    Hyeon Ho Kim
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 23:1743-8. 2009
    ..Our findings suggest a regulatory paradigm wherein HuR inhibits c-Myc expression by recruiting let-7-loaded RISC (RNA miRNA-induced silencing complex) to the c-Myc 3'UTR...
  86. pmc LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs
    Benedetta Ruzzenente
    Department of Mitochondrial Genetics, Max Planck Institute for Biology of Ageing, Cologne, Germany
    EMBO J 31:443-56. 2012
    ..Our findings point to the existence of an elaborate machinery that regulates mammalian mtDNA expression at the post-transcriptional level...
  87. ncbi The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta
    Fabio Martinon
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Mol Cell 10:417-26. 2002
    ..Thus, the inflammasome constitutes an important arm of the innate immunity...
  88. ncbi Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors
    X Luo
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
    Cell 94:481-90. 1998
    ..The cytochrome c releasing activity of Bid was antagonized by Bcl2. A mutation at the BH3 domain diminished its cytochrome c releasing activity. Bid, therefore, relays an apoptotic signal from the cell surface to mitochondria...
  89. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    ..Codepletion of both of these proteins in HeLa cells leads to the loss of ONM-associated nesp2G, as does overexpression of the Sun1 lumenal domain...
  90. pmc Plasma membrane contributes to the formation of pre-autophagosomal structures
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, UK
    Nat Cell Biol 12:747-57. 2010
    ....
  91. pmc Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis
    Sabrina L Spencer
    Center for Cell Decision Processes, Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 459:428-32. 2009
    ..Our results have implications for understanding 'fractional killing' of tumour cells after exposure to chemotherapy, and for variability in mammalian signal transduction in general...
  92. pmc The RING-CH ligase K5 antagonizes restriction of KSHV and HIV-1 particle release by mediating ubiquitin-dependent endosomal degradation of tetherin
    Claire Pardieu
    MRC Centre for Medical Molecular Virology, University College London, London, United Kingdom
    PLoS Pathog 6:e1000843. 2010
    ..Taken together our results demonstrate that K5 is a mechanistically distinct viral countermeasure to tetherin-mediated restriction, and that herpesvirus particle release is sensitive to this mode of antiviral inhibition...
  93. ncbi Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
    ..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
  94. pmc HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation
    Bastien Mangeat
    Department of Dermatology and Venereology, University Hospitals and Medical School of Geneva, University of Geneva, Switzerland
    PLoS Pathog 5:e1000574. 2009
    ..Identification of tetherin binding to Vpu provides a potential novel target for the development of drugs aimed at inhibiting HIV-1 replication...
  95. ncbi The Polycomb group protein EZH2 directly controls DNA methylation
    Emmanuelle Viré
    Free University of Brussels, Faculty of Medicine, Laboratory of Molecular Virology, 808 Route de Lennik, 1070 Brussels, Belgium
    Nature 439:871-4. 2006
    ..Our results suggest that EZH2 serves as a recruitment platform for DNA methyltransferases, thus highlighting a previously unrecognized direct connection between two key epigenetic repression systems...
  96. ncbi Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine 4
    Michiel Vermeulen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, D 82152 Martinsried, Germany
    Cell 131:58-69. 2007
    ..Our experiments reveal crosstalk between histone modifications and the transcription factor TFIID. This has important implications for regulation of RNA polymerase II-mediated transcription in higher eukaryotes...
  97. ncbi Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 316:1908-12. 2007
    ..Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events...
  98. pmc Molecular mechanism of BST2/tetherin downregulation by K5/MIR2 of Kaposi's sarcoma-associated herpesvirus
    Mandana Mansouri
    Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
    J Virol 83:9672-81. 2009
    ..work revealed that in the absence of VPU, HIV-1 virions are tethered to the plasma membrane in BST2-expressing HeLa cells. By targeting BST2, K5 might thus similarly overcome an innate antiviral host defense mechanism...
  99. ncbi Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
    Hideaki Tagami
    Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Cell 116:51-61. 2004
    ..This finding provides new insights into possible mechanisms for maintenance of epigenetic information after chromatin duplication...
  100. ncbi Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation
    H Zhou
    Division of Pathology and Laboratory Medicine, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nat Genet 20:189-93. 1998
    ..These findings suggest that STK15 is a critical kinase-encoding gene, whose overexpression leads to centrosome amplification, chromosomal instability and transformation in mammalian cells...
  101. ncbi Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing
    Ryan Morin
    Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada
    Biotechniques 45:81-94. 2008
    ..Analysis of these variants, and previously unannotated splicing events in the HeLa S3 cell line, reveals an overrepresentation of gene categories including those previously implicated in cancer...

Research Grants65

  1. Tat and CDK9 on gene expression alterations coupled to HIV-1 Associated Dementia
    Xavier Grana; Fiscal Year: 2010
    ..Since Tat reconfigures pools of CDK9 complexes in HIV-1 infected HeLa cells and Tat expressing primary human astrocytes and astrocytoma cells, it is likely that similar competition ..
  2. Cell Cycle Regulation and Adult T Cell Leukemia
    Chou Zen Giam; Fiscal Year: 2013
    ..abnormalities seen in Tax-expressing cells? Finally, the senescence-like arrest induced by HTLV-1 in SupT1 and HeLa cells suggests that primary T cells productively infected by HTLV-1 likely also undergo senescence...
  3. REGULATION OF NUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY
    Katherine L Wilson; Fiscal Year: 2012
    ..Interestingly, emerin interacts with the DNA damage-response kinase, DNA-PK, in vivo, and emerin-downregulated HeLa cells have reduced response to DNA damage, as assayed by the formation of phosphorylated-H2AX foci...
  4. Mechanisms of arsenic-induced chromosomal instability and carcinogenesis
    Wei Dai; Fiscal Year: 2013
    ..protein, leading to unscheduled activation of anaphase promoting complex/cyclosome (APC/C) in melanoma and HeLa cells. Activated APC/C apparently causes premature separation of sister chromatids, aberrant sister chromatid ..
  5. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..Aim 2 will establish the regulation of CHC22 intracellular recruitment to membranes in HeLa cells, human myoblasts and transfected 3T3-L1 cells to address the hypothesis that CHC22 influences GLUT4 transport in ..
  6. The role of intracellular trafficking pathways in calcium signaling
    Suzette Farber-Katz; Fiscal Year: 2013
    ..My sponsor's lab performed a genome-wide RNAi screen in HeLa cells to identify novel components of store-operated Ca2+ entry...
  7. Legionella pneumophila developmental cycle & virulence
    PAUL STOKES HOFFMAN; Fiscal Year: 2011
    ..an A/J mouse aerosol challenge model;and 3) To characterize the cyst like forms arising from protozoa,water and HeLa cells by (i) 2D gel proteome profiling for unique proteins, (ii)cyst fractionation studies to identify proteins of the ..
  8. Functions of 5'NCRs of picornavirus and cellular mRNAs
    Bert L Semler; Fiscal Year: 2013
    ..During poliovirus infection of HeLa cells, PCBP2 is cleaved by a viral proteinase to generate a truncated protein that is unable to function in ..
  9. Aminoglycoside microbicides restore natural expression of anti-HIV-1 retrocyclins
    ALEXANDER MICHAEL COLE; Fiscal Year: 2013
    ..In preliminary studies, we restored the expression of retrocyclin peptides by human promyelocytic and HeLa cells using aminoglycosides that suppressed retrocyclin's premature termination codon, and revealed that these ..
  10. Aminoglycoside microbicides restore natural expression of anti-HIV-1 retrocyclins
    Alexander M Cole; Fiscal Year: 2010
    ..In preliminary studies, we restored the expression of retrocyclin peptides by human promyelocytic and HeLa cells using aminoglycosides that suppressed retrocyclin's premature termination codon, and revealed that these ..
  11. Activation of Cyclin-Dependent Kinases by Fructose-2,6-Bisphosphate
    JASON A CHESNEY; Fiscal Year: 2013
    ..We then over-expressed PFKFB3 in HeLa cells and observed no change in glucose uptake but rather an increase in proliferation...
  12. The Role of the DNA Unwinding Element Binding Protein, DUE-B, in DNA Replication
    Michael Leffak; Fiscal Year: 2010
    ..We will use HeLa cells and Xenopus egg extracts to address several mechanistic aspects of the model in which DUE-B regulates the ..
  13. Targeting of 6-Phosphofructo-2 kinase in Cancer
    Jason Chesney; Fiscal Year: 2009
    ..We over-expressed PFKFB3 in HeLa cells and observed no change in glucose uptake but rather an increase in cell proliferation as well as the expression ..
  14. Functional Coupling of Steps in Genes Expression
    ROBIN E REED; Fiscal Year: 2013
    ..SMN-containing nuclear Gems are deficient in FUS knockdown HeLa cells and in ALS patient fibroblasts with a FUS mutation...
  15. The RAN GTPase
    Ian G Macara; Fiscal Year: 2013
    ..We will use cytoplasmic extracts to purify such an enzyme activity. Finally, we discovered that in HeLa cells, which express the E7 oncoprotein, RB is not detectably methylated...
  16. Mechanisms for cell uptake of LDL/DNA complexes
    NATALIA V GUEVARA; Fiscal Year: 2013
    ..Synthetic peptides spanning these regions of Apo B100 were shown to bind or enter HeLa cells in our preliminary studies...
  17. Integrated Analyses of Taspase 1 (MLL Cleaving Protease)
    James J D Hsieh; Fiscal Year: 2010
    ..Preliminary studies in HeLa cells indicated the importance of Taspasel-mediated MLL cleavage in HOX gene expression...
  18. UAB Multi-user Fermentation Facility Upgrade
    NORMAN P HIGGINS; Fiscal Year: 2010
    ..Kim Hardy has been a key Research Assistant for 12 years. Kim took charge of growing 2000 L of HeLa cells that allowed Hengbin Wang to isolate a deubiquitination complex (see Joo, H. Y., L. Zhai, et al. (2007)...
  19. Systems Architecture And Dynamical Behaviors of the Kinases That Drive M-Phase
    JOSEPH RICHARD POMERENING; Fiscal Year: 2013
    ..has on M-phase kinase regulation and cell cycle progression will be investigated using single-cell methods in HeLa cells, such as flow cytometry and live-cell imaging...
  20. Screening strategy for small molecules inhibitors of cancer
    Paul B Fisher; Fiscal Year: 2012
    ..First, we will screen a library of chemicals employing a luciferase cell-based assay with HeLa cells stably expressing the PEG-Promoter upstream of firefly luciferase...
  21. 3-Dimensional Imaging of Biomaterials on the Nanoscale with Mass Spectrometry
    Nicholas Winograd; Fiscal Year: 2010
    ..Representative projects include elucidation of de novo purine biosynthetic pathways in HeLa cells, assay of single vesicles in neurotransmitters, lipid biochemistry at model membranes, heart tissue and in ..
  22. Role of MN1-TEL and MN1 in Leukemogenesis
    Gerard C Grosveld; Fiscal Year: 2010
    ..with the transcription coactivators p300 and RAC and stimulates transcription of the retinoic acid receptor in HeLa cells, we know little about the mechanistic pathways that involve MN1 and MN1-TEL in myeloid cells...
  23. Functional characterization of Rho inactivation by Vibro cholerae RTX toxin
    BRETT L GEISSLER; Fiscal Year: 2010
    ..analysis to establish the portions of RID that are required for localization to the plasma membrane of HeLa cells. 2) Identify binding partners for MARTXVc RID within eukaryotic cells...
  24. Illuminating the Mechanistic Pathways of Polymer-Mediated Nucleic Acid Delivery
    Theresa M Reineke; Fiscal Year: 2010
    ..forms: plasmid DNA, oligodeoxynucleotide decoys, and small interfering RNA, in two cell types, H9C2(2-1) and Hela cells. The research program highlighted herein is driven by three specific goals: 1) to unravel the molecular-level ..
  25. ASSEMBLY AND TRANSFER OF N-LINKED OLIGOSACCHARIDES
    JAMES REID GILMORE; Fiscal Year: 2013
    ..Assembly of Dol-PP-OS will be perturbed in HeLa cells by si-RNA mediated depletion of the ALG6 glucosyltransferase...
  26. Regulators of Calcium Signaling
    JOHN ANTHONY PUTKEY; Fiscal Year: 2013
    ..We also show that PEP-19 sensitizes HeLa cells to ATP-dependent Ca2+ release, and that this dependent on its ability to modulate the Ca2+ binding properties of ..
  27. Alternative splicing regulation in perspective: A global analysis of exon skippin
    LUIZ OTAVIO PENALVA; Fiscal Year: 2010
    ..In this process, an "alternative exon" is either excluded or included in the mature mRNA transcript. HeLa cells will be used as a model system and 6 nuclear RNA binding proteins (RBPs) will be selected for analysis...
  28. INTERCELLULAR COMMUNICATION IN THE LENS
    Lisa Ebihara; Fiscal Year: 2013
    ..We will use this preparation as well as HeLa cells transfected with different lens connexins to address the following questions: What is the molecular identity of ..
  29. Disruption of Reelin biosynthesis by de novo missense mutations found in aut
    DAWN LAMMERT; Fiscal Year: 2013
    ..Aim 1: Preliminary studies show mutant Reelin is absent in the media of transfected HeLa cells. Pulse-chase experiments will elucidate whether this is due to a secretion defect, innate protein instability, ..
  30. Development of anti-Influenza agents by a systems biology approach
    Ari Helenius; Fiscal Year: 2011
    ..Automated, primary, 7000 gene siRNA silencing screens using a human influenza A virus (X31/Aichi/68), HeLa cells and human airway epithelial cells, and a high-content, microscopy-based assay are already in progress...
  31. Mechanisms of spindle formation
    Phong T Tran; Fiscal Year: 2013
    ..We have begun to characterize a novel human gene we called PSR1. In HeLa cells, siRNA of PSR1 also leads to high frequency of monopolar spindles and subsequent chromosome segregation defects...
  32. Role of Huntington's Disease Protein in Post-Transcriptional Gene Silencing
    Naoko Tanese; Fiscal Year: 2013
    ..insight into Htt function and uncover potential HD pathogenic mechanisms, epitope-tagged Htt was purified from HeLa cells and Argonaute (Ago) proteins were identified as Htt-interacting proteins...
  33. KINETOCHORE STRUCTURE AND FUNCTION
    TIMOTHY YEN; Fiscal Year: 2010
    ..of the Mitotic Checkpoint Complex (MCC) as the major inhibitor of the Anaphase Promoting Complex (APC) in Hela cells led to a new two-step model for how APC is inhibited by the checkpoint...
  34. GLYCOSYLATION AND CANCER
    Gerald Warren Hart; Fiscal Year: 2013
    ..A. Using short-term treatments of HeLa cells with a general kinase inhibitor or a general phosphatase inhibitor, combined with novel quantitative glyco-..
  35. CELLULAR RESTRICTIVE FACTOR TARGETED BY VIRAL PROTEIN X
    Lee Ratner; Fiscal Year: 2012
    ..Potential restriction factor cDNAs will be examined for the ability to inhibit infection of HeLa cells by HIV-2 Vpx but not wild type HIV-2...
  36. MRP2, BSEP, and BCRP Transporter Polymorphisms and Chemotherapy Disposition
    RICHARD HSINSHIN HO; Fiscal Year: 2011
    ..will be functionally characterized utilizing in vitro recombinant vaccinia-mediated transfection assays in HeLa cells and immunoblot and confocal immunofluoresecent analyses to evaluate total and cell surface protein expression...
  37. Cell-and Nucleus-Permeable Luminescent Gold Nanoparticles
    Jie Zheng; Fiscal Year: 2010
    ..We recently found that highly luminescent, gold nanoparticles about 2 nm in diameter when exposed to HeLa cells rapidly crossed cellular membranes and nuclear membranes apparently by passive diffusion and subsequently bound ..
  38. Mucosal Cell Transporters and Enzymes for Enhancing Oral Drug Absorption
    Gordon L Amidon; Fiscal Year: 2013
    ..2. Determine the mucosal transport and activation of the prodrugs, evaluate the uptake in HeLa cells, transport permeability and activation in Caco-2 monolayers, and in vivo in the in situ perfused rat jejunum...
  39. The Role of Ceramidases in Cancer Chemotherapy
    Cungui Mao; Fiscal Year: 2013
    ..ceramidase 2 (ACER2) markedly enhances the cytotoxicity of doxorubicin, a known ceramide-inducing ACCA, in HeLa cells, whereas knocking down ACER2 has the opposite effect...
  40. MicroRNA Expression Profiling Circuits for Detection and Destruction of Cancer
    Ron Weiss; Fiscal Year: 2013
    ..For example, for selective targeting of HeLa cells we require expression of a pro-apoptotic protein only when microRNAs miR-21, miR-17, and miR-30a are over-..
  41. Synthesis and Biology of Phorboxazole
    Craig J Forsyth; Fiscal Year: 2012
    ..derivatives of phorboxazole A have been developed and used to isolate phorboxazole analog binding proteins from HeLa cells. Phorboxazole analogs were independently found to bind to cyclin dependent kinase 4 (cdk4), cytokeratins, and ..
  42. PEPTIDE BASED SALMONELLA VACCINE DELIVERY SYSTEM
    Lee Riley; Fiscal Year: 1999
    ..coli beta- galactosidase gene was cloned into pInv3 and its product was shown to efficient associate with HeLa cells, while the enzyme beta-galactosidase without the Inv3 at its N-terminus failed to associate with HeLa cells...
  43. Roles of Glycoslyation in Notch Signaling
    PAMELA M STANLEY; Fiscal Year: 2013
    ..modification of Notch1 EGF repeats by O-GlcNAc is required for optimal ligand-induced activation of Notch1 in HeLa cells. Specific Aim 3 will determine how O-GlcNAc is important for Notch1 signaling in mammalian cells and in the ..
  44. Molecular Mechanism of LEDGF/p75 in HIV Integration
    Manuel Llano; Fiscal Year: 2010
    ..Integrase-eGFP fusion protein will be expressed in HeLa cells;drugs interfering with LEDGF/p75-integrase interaction are expected to reduce eGFP expression in these cells.
  45. PEPTIDE BASED DELIVERY OF HIV IMMUNOGEN
    Lee Riley; Fiscal Year: 1999
    ..green fluorescent protein, and ovalbumin expressed in fusion with Inv3 are able to abundantly associate with HeLa cells. We plan to express gp120 using pInv3 to deliver the protein into mammalian cells to study the efficiency of its ..
  46. Role of host cell metabolism in supporting intracellular Trypanosma cruzi growth
    Barbara A Burleigh; Fiscal Year: 2013
    ..cruzi amastigotes in HeLa cells. We also made the observation that intracellular T...
  47. Functional and Pharmacological Implications of mGluR Heteromerization
    Paul J Kammermeier; Fiscal Year: 2013
    ..system, 2) To confirm the pharmacological and signaling properties of mGluR1/5 heteromers by expression in HeLa cells and using a different effectors to assay receptor signaling, 3) To directly assess the ability of fluorescently-..
  48. Bistability and the Mitotic Trigger
    James E Ferrell; Fiscal Year: 2012
    ..The proposed approaches include quantitative experimental studies in HeLa cells;Xenopus egg extracts, and reconstituted in vitro systems;fluorescent biosensor development;and computational ..
  49. The Role of Rab24 in HIV-1 Assembly
    ARMANDO DEL PORTILLO; Fiscal Year: 2010
    ..We have observed colocalization between HIV Gag and Rab24 or a Rab24 mutant in 293T and HeLa cells. We aim to determine the subcellular localization of these proteins in macrophages and CD4+ T-cells by confocal ..
  50. Chemical and Biological Mechanisms of Leinamycin
    Kent S Gates; Fiscal Year: 2010
    ..g. IC50 of 0.014 ng/mL - this translates to an IC50 of 27 nM - against HeLa cells) and is currently in development as a potential anticancer agent at Kyowa Hakko Kogyo Pharmaceuticals...
  51. A feasibility study of genetically encoded fluorescent gold nanoclusters
    Jan Liphardt; Fiscal Year: 2009
    ..Scaffolded peptide libraries will be expressed in HeLa cells. Cells that become fluorescent upon gold exposure will be isolated by fluorescence-activated cell-sorting (FACS)...
  52. Impact of Arsenicals on Selenoprotein Synthesis
    William Self; Fiscal Year: 2006
    ..of arsenite inhibited the incorporation of selenium into selenoproteins in both keratinocytes (HaCat) and HeLa cells. Based on these observations, our hypothesis is that the increase in ROS observed in cells treated with ..
  53. THE MECHANISM AND FUNCTION OF A TO I RNA EDITING
    Jinghua Yang; Fiscal Year: 2004
    ..Specifically, using HeLa cells we have shown that glutamate receptor subunit B (gluR-B) mRNA can undergo site specific deamination at an ..
  54. PATHOGENESIS OF LIVER CELL NECROSIS
    John Farber; Fiscal Year: 2005
    ..process that results in induction of the MPT? In those cells that contain both Bax and Bid (L929 fibroblasts and HeLa cells), we propose to distinguish between two hypotheses. Bax and Bid are redundant proteins and act independently...
  55. VACCINIA VIRUS LATE TRANSCRIPTION FACTOR
    Cynthia Wright; Fiscal Year: 2003
    ..assays were used to demonstrate that VLTF-X activity is present in the cytoplasm and nucleus of uninfected HeLa cells. VLTF-X activity from uninfected cells is indistinguishable from that recovered from infected cells by a variety ..
  56. CARBOHYDRATE BINDING PROTEIN 35
    John Wang; Fiscal Year: 2006
    ..Using nuclear extracts (NE) derived from HeLa cells, capable of carrying out splicing of pre-mRNA in a cell-free assay, depletion and reconstitution experiments ..
  57. POLYADENYLATION OF MESSENGER RNA PRECURSORS REGULATION
    PHILLIP SHARP; Fiscal Year: 1990
    ..We have recently developed a soluble reaction from extracts of Hela cells that accurately polyadenylates exogenous substrate RNAs...
  58. REPLICON MISFUNCTIONS IN HUMAN CARCINOGENESIS
    JEAN MICHEL VOS; Fiscal Year: 1999
    ..bypass deficient XPV cell-free extracts using purified replication/repair factors or fractionated extracts from HeLa cells will be developed...
  59. VIRAL INTERFERENCE IN CARRIER CULTURES
    Richard Crowell; Fiscal Year: 1992
    ..A second receptor (HR2) for binding the CB-RD variant viruses to RD cells and to HeLA cells has been discovered...
  60. MOLECULAR BIOLOGY OF INFECTION WITH M TUBERCULOSIS
    Lee Riley; Fiscal Year: 2002
    ..cytoskeletal rearrangement (cytochalasin D, nocodazole, and onodansylcadaverine) inhibited the association with HeLa cells of microspheres coated with Mcep1...
  61. BIOCHEMICAL ANALYSIS OF FACTORS ASSOCIATED WITH TFIID
    Naoko Tanese; Fiscal Year: 1999
    ..For analytical purposes, subunits of TFIID will be transiently expressed in HeLa cells using the infection/transfection protocol and complex assembly will be monitored by pulse-labeling and ..
  62. Real-time measurement of ethanol's effect on cyclic AMP metabolism in live cells
    Masami Yoshimura; Fiscal Year: 2009
    ..In Specific Aim 1, we will determine the effects of ethanol on cAMP metabolism in HeLa cells. Using a FRET-based cAMP sensor, Epac1-camps, the level of cAMP in HeLa cells will be monitored in real-time at ..
  63. PROCESSING OF PRECURSORS TO MRNA IN DROSPHILA
    STEPHEN MOUNT; Fiscal Year: 1999
    ..its ability to function as a basal splicing factor in complementation of S100 extracts from Drosophila or Hela cells and tests of its ability to alter 5' splice site choice in appropriate globin constructs (the canonical SR ..
  64. POLIOVIRUS USING INFECTIOUS VIRAL CDNA CLONE
    VINCENT RACANIELLO; Fiscal Year: 2002
    ..to neutralization with soluble receptor (srr mutants) have been isolated which have reduced binding affinity for HeLa cells. These mutants will be studied to identify capsid residues that control poliovirus binding, transition to ..
  65. GENE TRANSFER TO AIRWAY EPITHELIAL CELLS
    Pi Wan Cheng; Fiscal Year: 2000
    ..vector composed of a receptor ligand and a cationic liposome, which yields high transfection efficiency in HeLa cells and immortalized tracheal epithelial cells of a cystic fibrosis patient (CFT1)...