enterocytes

Summary

Summary: Absorptive cells in the lining of the INTESTINAL MUCOSA. They are differentiated EPITHELIAL CELLS with apical MICROVILLI facing the intestinal lumen. Enterocytes are more abundant in the SMALL INTESTINE than in the LARGE INTESTINE. Their microvilli greatly increase the luminal surface area of the cell by 14- to 40 fold.

Top Publications

  1. ncbi gp130-mediated Stat3 activation in enterocytes regulates cell survival and cell-cycle progression during colitis-associated tumorigenesis
    Julia Bollrath
    2nd Department of Medicine, Klinikum rechts der Isar, Technical University Munich, Munich, Germany
    Cancer Cell 15:91-102. 2009
  2. pmc The proteome of cytosolic lipid droplets isolated from differentiated Caco-2/TC7 enterocytes reveals cell-specific characteristics
    Julien Bouchoux
    Universite Pierre et Marie Curie Paris 6, UMR S 872, Les Cordeliers, Paris 75006, France
    Biol Cell 103:499-517. 2011
  3. ncbi Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption
    Scott W Altmann
    Department of Cardiovascular Endocrine Research, Schering Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ, 07033 0539, USA
    Science 303:1201-4. 2004
  4. ncbi Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes
    Frederic Dalle
    Robert Koch Institute, Nordufer 20, Berlin, Germany
    Cell Microbiol 12:248-71. 2010
  5. pmc Gliadin-mediated proliferation and innate immune activation in celiac disease are due to alterations in vesicular trafficking
    M Vittoria Barone
    Department of Pediatrics, European Laboratory for the Investigation of Food Induced Diseases, University of Naples Federico II, Naples, Italy
    PLoS ONE 6:e17039. 2011
  6. ncbi The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics
    Y Sambuy
    Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione INRAN, CNR, Roma, Italy
    Cell Biol Toxicol 21:1-26. 2005
  7. pmc The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration
    Phillip Karpowicz
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 137:4135-45. 2010
  8. pmc Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium
    Misty Good
    Division of Newborn Medicine, Children s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA
    Proc Natl Acad Sci U S A 109:11330-5. 2012
  9. pmc The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes
    James Harper
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Proc Natl Acad Sci U S A 108:10585-90. 2011
  10. pmc A novel member of a zinc transporter family is defective in acrodermatitis enteropathica
    Kun Wang
    Howard Hughes Medical Institute and Department of Medicine, University of California, San Francisco, 94143, USA
    Am J Hum Genet 71:66-73. 2002

Detail Information

Publications348 found, 100 shown here

  1. ncbi gp130-mediated Stat3 activation in enterocytes regulates cell survival and cell-cycle progression during colitis-associated tumorigenesis
    Julia Bollrath
    2nd Department of Medicine, Klinikum rechts der Isar, Technical University Munich, Munich, Germany
    Cancer Cell 15:91-102. 2009
    ....
  2. pmc The proteome of cytosolic lipid droplets isolated from differentiated Caco-2/TC7 enterocytes reveals cell-specific characteristics
    Julien Bouchoux
    Universite Pierre et Marie Curie Paris 6, UMR S 872, Les Cordeliers, Paris 75006, France
    Biol Cell 103:499-517. 2011
    Intestinal absorption of alimentary lipids is a complex process ensured by enterocytes and leading to TRL [TAG (triacylglycerol)-rich lipoprotein] assembly and secretion...
  3. ncbi Niemann-Pick C1 Like 1 protein is critical for intestinal cholesterol absorption
    Scott W Altmann
    Department of Cardiovascular Endocrine Research, Schering Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ, 07033 0539, USA
    Science 303:1201-4. 2004
    ..NPC1L1 expression is enriched in the small intestine and is in the brush border membrane of enterocytes. Although otherwise phenotypically normal, NPC1L1-deficient mice exhibit a substantial reduction in absorbed ..
  4. ncbi Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes
    Frederic Dalle
    Robert Koch Institute, Nordufer 20, Berlin, Germany
    Cell Microbiol 12:248-71. 2010
    ..albicans with oral epithelial cells and enterocytes. Our data demonstrate that adhesion, invasion and damage by C...
  5. pmc Gliadin-mediated proliferation and innate immune activation in celiac disease are due to alterations in vesicular trafficking
    M Vittoria Barone
    Department of Pediatrics, European Laboratory for the Investigation of Food Induced Diseases, University of Naples Federico II, Naples, Italy
    PLoS ONE 6:e17039. 2011
    ..immune responses, with IL-15 as a major mediator of the innate immune response, and by proliferation of crypt enterocytes as an early alteration of CD mucosa causing crypts hyperplasia...
  6. ncbi The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics
    Y Sambuy
    Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione INRAN, CNR, Roma, Italy
    Cell Biol Toxicol 21:1-26. 2005
    ..Since the use of Caco-2 cells has grown exponentially in recent years, it is particularly important to highlight these methodological aspects in order to promote the standardization and optimisation of this intestinal model...
  7. pmc The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration
    Phillip Karpowicz
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Development 137:4135-45. 2010
    ..These findings demonstrate a cell-autonomous role for the Hippo pathway in SCs, and have implications for understanding the role of this pathway in tumorigenesis and cancer stem cells...
  8. pmc Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium
    Misty Good
    Division of Newborn Medicine, Children s Hospital of Pittsburgh, Pittsburgh, PA 15224, USA
    Proc Natl Acad Sci U S A 109:11330-5. 2012
    ..fluid did not prevent TLR4 signaling in EGFR- or peroxisome proliferator-activated receptor γ-deficient enterocytes or in mice deficient in intestinal epithelial EGFR, and purified EGF attenuated the exaggerated intestinal ..
  9. pmc The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes
    James Harper
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Proc Natl Acad Sci U S A 108:10585-90. 2011
    ..Intestinal enterocytes dramatically alter their biochemical signature during the suckling-to-weaning transition...
  10. pmc A novel member of a zinc transporter family is defective in acrodermatitis enteropathica
    Kun Wang
    Howard Hughes Medical Institute and Department of Medicine, University of California, San Francisco, 94143, USA
    Am J Hum Genet 71:66-73. 2002
    ..We show that Slc39A4 is abundantly expressed in mouse enterocytes and that the protein resides in the apical membrane of these cells...
  11. ncbi Active sugar transport in health and disease
    E M Wright
    Department of Physiology, The David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1751, USA
    J Intern Med 261:32-43. 2007
    ..SGLT1 plays a central role in Oral Rehydration Therapy used so effectively to treat secretory diarrhoea such as cholera. Increasing attention is being focused on SGLTs as drug targets for the therapy of diabetes...
  12. pmc Transcriptional modulation of genes encoding structural characteristics of differentiating enterocytes during development of a polarized epithelium in vitro
    Jennifer M Halbleib
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    Mol Biol Cell 18:4261-78. 2007
    ..of human Caco-2 cells in tissue culture, which develop structural and functional polarity similar to that of enterocytes in vivo...
  13. pmc beta-Klotho and FGF-15/19 inhibit the apical sodium-dependent bile acid transporter in enterocytes and cholangiocytes
    Jyoti Sinha
    Department of Pediatrics, Mount Sinai School of Medicine, New York, NY, USA
    Am J Physiol Gastrointest Liver Physiol 295:G996-G1003. 2008
    ..These results indicate that both beta-Klotho and FGF-15/19 repress ASBT in enterocytes and cholangiocytes. These novel signaling pathways need to be considered in analyzing bile acid homeostasis.
  14. ncbi Dietary fat sensing via fatty acid oxidation in enterocytes: possible role in the control of eating
    Wolfgang Langhans
    Physiology and Behaviour Laboratory, Institute of Food Nutrition and Health, Zurich, Schwerzenbach, Switzerland
    Am J Physiol Regul Integr Comp Physiol 300:R554-65. 2011
    ..Finally, cell culture studies indicate that enterocytes oxidize fatty acids, which can be modified pharmacologically...
  15. pmc Type 1 fimbriae of Salmonella enterica serovar Typhimurium bind to enterocytes and contribute to colonization of swine in vivo
    Carrie Althouse
    Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois 61802, USA
    Infect Immun 71:6446-52. 2003
    ..This strain also is known to exist in two phenotypes (adhesive and nonadhesive to enterocytes) and can switch between the two phenotypes at a rate consistent with phase variation...
  16. pmc A dynamic, cytoplasmic triacylglycerol pool in enterocytes revealed by ex vivo and in vivo coherent anti-Stokes Raman scattering imaging
    Jiabin Zhu
    Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
    J Lipid Res 50:1080-9. 2009
    The absorptive cells of the small intestine, enterocytes, are not generally thought of as a cell type that stores triacylglycerols (TGs) in cytoplasmic lipid droplets (LDs)...
  17. ncbi JAK/STAT signaling coordinates stem cell proliferation and multilineage differentiation in the Drosophila intestinal stem cell lineage
    Katherine Beebe
    Washington University School of Medicine, Department of Developmental Biology, Campus Box 8103, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Dev Biol 338:28-37. 2010
    ....
  18. pmc Toll-like receptor-4 inhibits enterocyte proliferation via impaired beta-catenin signaling in necrotizing enterocolitis
    Chhinder P Sodhi
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15224, USA
    Gastroenterology 138:185-96. 2010
    ..Given the importance of beta-catenin in regulating proliferation of many cell types, we hypothesize that TLR4 impairs enterocyte proliferation in NEC via impaired beta-catenin signaling...
  19. ncbi Collagenase-1 (MMP-1), matrilysin-1 (MMP-7), and stromelysin-2 (MMP-10) are expressed by migrating enterocytes during intestinal wound healing
    M T Salmela
    Department of Dermatology, Helsinki University Central Hospital and Biomedicum, FI 00250 Helsinki, Finland
    Scand J Gastroenterol 39:1095-104. 2004
    ....
  20. ncbi HMGB1 is secreted by immunostimulated enterocytes and contributes to cytomix-induced hyperpermeability of Caco-2 monolayers
    Shiguang Liu
    Department of Critical Care Medicine, Univ of Pittsburgh School of Medicine, 616 Scaife Hall, 3550 Terrace St, PA 15261, USA
    Am J Physiol Cell Physiol 290:C990-9. 2006
    ..We hypothesized that immunostimulated enterocytes might be another source for this mediator...
  21. pmc Gliadin peptide P31-43 localises to endocytic vesicles and interferes with their maturation
    Maria Vittoria Barone
    Pediatric Department and European Laboratory for the Investigation of Food Induced Disease, University of Naples Federico II, Naples, Italy
    PLoS ONE 5:e12246. 2010
    ..Growth Factor Receptor (EGFR)-dependent actin remodelling and proliferation in cultured cell lines and in enterocytes from CD patients...
  22. pmc Bovine prion is endocytosed by human enterocytes via the 37 kDa/67 kDa laminin receptor
    Etienne Morel
    UMR505 INSERM UPMC, 15 rue de l Ecole de Medecine, 75006 Paris, France
    Am J Pathol 167:1033-42. 2005
    ..We have thus analyzed the early mechanisms that could account for an uptake of infectious prion particles by enterocytes, the major cell population of the intestinal epithelium...
  23. ncbi DMT1 gene expression and cadmium absorption in human absorptive enterocytes
    J Tallkvist
    Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 573, SE 751 23, Uppsala, Sweden
    Toxicol Lett 122:171-7. 2001
    ..absorption process and gene expression of DMT1 was investigated in an experimental model of human absorptive enterocytes. Fully differentiated Caco-2 cells were grown in monolayers and treated with iron supplemented medium or ..
  24. ncbi The role of enterocytes in the intestinal barrier function and antigen uptake
    Veerle Snoeck
    Laboratory of Veterinary Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    Microbes Infect 7:997-1004. 2005
    ..The cell polarity and structural properties of the enterocytes, limiting the amount of antigen reaching the epithelial surface, form the basis of the integrity of the ..
  25. ncbi Effect of the mutation (C3435T) at exon 26 of the MDR1 gene on expression level of MDR1 messenger ribonucleic acid in duodenal enterocytes of healthy Japanese subjects
    Tsutomu Nakamura
    Department of Hospital Pharmacy, School of Medicine, Kobe University, Japan
    Clin Pharmacol Ther 71:297-303. 2002
    ..This finding suggested a lower plasma concentration of the substrates for CYP3A4 in subjects harboring the C3435T mutation of the MDR1 gene...
  26. pmc Simple-sugar meals target GLUT2 at enterocyte apical membranes to improve sugar absorption: a study in GLUT2-null mice
    F Gouyon
    INSERM U505, UPMC, Paris, France
    J Physiol 552:823-32. 2003
    ..Thus, GLUT2 and GLUT5 could exert complementary roles in adapting the absorption capacity of the intestine to occasional or repeated loads of dietary sugars...
  27. ncbi Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes
    Ben A V Thompson
    School of Medicine, University of East Anglia, Norwich NR4 7TJ, UK
    J Agric Food Chem 58:8414-7. 2010
    ..No change was seen in ferroportin expression. Our data suggest that calcium reduces iron bioavailability by decreasing DMT-1 expression at the apical cell membrane, thereby downregulating iron transport into the cell...
  28. ncbi Gene expression profiling of Caco-2 BBe cells suggests a role for specific signaling pathways during intestinal differentiation
    James C Fleet
    Interdepartmental Nutrition Program, Purdue University, West Lafayette, Indiana 47907
    Physiol Genomics 13:57-68. 2003
    ....
  29. ncbi An undigested gliadin peptide activates innate immunity and proliferative signaling in enterocytes: the role in celiac disease
    Merlin Nanayakkara
    Department of Pediatrics and European Laboratory for the Investigation of Food Induced Disease, University of Naples, Federico II, Naples, Italy, and the Department of Medicine, University of Chicago, Chicago, IL
    Am J Clin Nutr 98:1123-35. 2013
    ..component of wheat and other cereals, the celiac intestine is characterized by the proliferation of crypt enterocytes with an inversion of the differentiation/proliferation program...
  30. pmc Transactivation of EGFR by LPS induces COX-2 expression in enterocytes
    Steven J McElroy
    Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America
    PLoS ONE 7:e38373. 2012
    ..Taken together, these data show that EGFR plays an important role in LPS-induction of COX-2 expression in enterocytes, which may be one mechanism for EGF in inhibition of NEC.
  31. pmc Enterocytes: active cells in tolerance to food and microbial antigens in the gut
    N Miron
    Department of Immunology, University of Medicine and Pharmacy, Iuliu Hatieganu Cluj Napoca, Romania
    Clin Exp Immunol 167:405-12. 2012
    b>Enterocytes used to be studied particularly in terms of digestion protagonists...
  32. pmc Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes
    Irina Kadiyala
    Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA
    Eur J Pharm Sci 39:103-9. 2010
    ..Using two different in vitro cellular models to partially reproduce the characteristics of intestinal enterocytes and M-cells, this study demonstrates that nanoparticle transport through the M-cell co-culture model is 5-fold ..
  33. ncbi Dietary zinc absorption is mediated by ZnT1 in Drosophila melanogaster
    Xiaoxi Wang
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China
    FASEB J 23:2650-61. 2009
    ..expressed in restricted regions of the midgut and exhibited a distribution on the basolateral membrane of the enterocytes. Gut-specific silencing of dZnT1 was sufficient to evoke lethality under zinc scarcity...
  34. pmc Beta-1,2- and alpha-1,2-linked oligomannosides mediate adherence of Candida albicans blastospores to human enterocytes in vitro
    Frederic Dalle
    Laboratoire de Parasitologie Mycologie, Hopital du Bocage, Dijon, France
    Infect Immun 71:7061-8. 2003
    ..Taken together, our data indicate that alpha-1,2 and beta-1,2 oligomannosides are involved in the C. albicans-enterocyte interaction and participate in the adhesion of the yeasts to the mucosal surface...
  35. pmc Pdx1 inactivation restricted to the intestinal epithelium in mice alters duodenal gene expression in enterocytes and enteroendocrine cells
    Chin Chen
    Stanford Univ School of Medicine, CA 94305 5208, USA
    Am J Physiol Gastrointest Liver Physiol 297:G1126-37. 2009
    ..We conclude that Pdx1 is necessary for patterning appropriate gene expression in enterocytes and enteroendocrine cells of the proximal small intestine.
  36. pmc Influence of class B scavenger receptors on cholesterol flux across the brush border membrane and intestinal absorption
    David V Nguyen
    Gastroenterology, Hepatology, and Nutrition Division, Children s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4318, USA
    J Lipid Res 50:2235-44. 2009
    To learn more about how the step of cholesterol uptake into the brush border membrane (BBM) of enterocytes influences overall cholesterol absorption, we measured cholesterol absorption 4 and 24 h after administration of an intragastric ..
  37. pmc Reciprocal expression and signaling of TLR4 and TLR9 in the pathogenesis and treatment of necrotizing enterocolitis
    Steven C Gribar
    Department of Surgery, Division of Pediatric Surgery, Children s Hospital of Pittsburgh, and University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Immunol 182:636-46. 2009
    ..Activation of TLR9 with CpG-DNA inhibited LPS-mediated TLR4 signaling in enterocytes in a mechanism dependent upon the inhibitory molecule IRAK-M...
  38. ncbi Functional differences between M cells and enterocytes in sampling luminal antigens
    Jennelle M Kyd
    Central Queensland University, Rockhampton, Queensland 4702, Australia
    Vaccine 26:6221-4. 2008
    ..A number of these PRRs are also found on neighbouring enterocytes and therefore the pathways signalled by receptor-ligand binding may differentially trigger different ..
  39. ncbi The role of innate immune-stimulated epithelial apoptosis during gastrointestinal inflammatory diseases
    Richard H Siggers
    Division of Pediatric Surgery, Children s Hospital of Pittsburgh of UPMC, Pittsburgh, PA 15224, USA
    Cell Mol Life Sci 68:3623-34. 2011
    ..This review will detail the regulatory pathways that govern enterocyte apoptosis, and will explore the role of the innate immune system in the induction of enterocyte apoptosis in gastrointestinal disease...
  40. pmc CD36 deficiency impairs intestinal lipid secretion and clearance of chylomicrons from the blood
    Victor A Drover
    Department of Physiology and Biophysics, State University of New York at Stony Brook, USA
    J Clin Invest 115:1290-7. 2005
    ..The secretion defect appeared to reflect an impaired ability of CD36-null enterocytes to efficiently synthesize triacylglycerols from dietary FAs in the endoplasmic reticulum...
  41. pmc Saccharomyces boulardii improves intestinal cell restitution through activation of the α2β1 integrin collagen receptor
    Alexandra Canonici
    INSERM, UMR 911, Centre de Recherche en Oncologie et Oncopharmacologie, Marseille, France
    PLoS ONE 6:e18427. 2011
    ..Complete remission of these diseases requires both the cessation of inflammation and the migration of enterocytes to repair the damaged epithelium...
  42. pmc Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption
    Dong Cheng
    Department of Metabolic Diseases, Bristol Myers Squibb Company, Princeton, New Jersey 08543 5400, USA
    J Biol Chem 283:29802-11. 2008
    ..It also decreased TAG and DAG syntheses in primary enterocytes. Last, when delivered orally to rats, XP620 decreased absorption of orally administered lipids by approximately ..
  43. pmc Prion uptake in the gut: identification of the first uptake and replication sites
    Pekka Kujala
    Section of Cell Biology II, Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS Pathog 7:e1002449. 2011
    ..was transiently detectable at 1 day post feeding (dpf) within large multivesicular LAMP1-positive endosomes of enterocytes in the follicle-associated epithelium (FAE) and at much lower levels within M cells...
  44. pmc Peyer's patch-deficient mice demonstrate that Mycobacterium avium subsp. paratuberculosis translocates across the mucosal barrier via both M cells and enterocytes but has inefficient dissemination
    Luiz E Bermudez
    Kuzell Institute, California Pacific Medical Center Research Institute, San Francisco, California 94115, USA
    Infect Immun 78:3570-7. 2010
    ..avium subsp. paratuberculosis enters the intestinal mucosa through enterocytes in the absence of M cells. In addition, the results indicated that M. avium subsp...
  45. ncbi Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
    A Donovan
    Department of Medicine, Children s Hospital and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 403:776-81. 2000
    ..Iron uptake from the intestinal lumen through the apical surface of polarized duodenal enterocytes is mediated by the divalent metal transporter, DMTi...
  46. pmc Nonautonomous regulation of Drosophila midgut stem cell proliferation by the insulin-signaling pathway
    Na Hyun Choi
    Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:18702-7. 2011
    ..adult midgut intestinal stem cells (ISCs) maintain tissue homeostasis by producing progeny that replace dying enterocytes and enteroendocrine cells...
  47. pmc Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut
    Huaqi Jiang
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Cell 137:1343-55. 2009
    ..We show here that when enterocytes (ECs) in the Drosophila midgut are subjected to apoptosis, enteric infection, or JNK-mediated stress signaling, ..
  48. ncbi Differential association of adipophilin and TIP47 proteins with cytoplasmic lipid droplets in mouse enterocytes during dietary fat absorption
    Bonggi Lee
    Department of Foods and Nutrition, Purdue University, 700 W State Street, West Lafayette, IN 47907 2059, USA
    Biochim Biophys Acta 1791:1173-80. 2009
    Recently, we found that enterocytes dynamically store triglycerides (TGs) in cytoplasmic lipid droplets (CLDs) during dietary fat absorption...
  49. ncbi Requirement of Math1 for secretory cell lineage commitment in the mouse intestine
    Q Yang
    Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Science 294:2155-8. 2001
    ..small intestinal epithelium consists of four principal cell types deriving from one multipotent stem cell: enterocytes, goblet, enteroendocrine, and Paneth cells...
  50. pmc A transient niche regulates the specification of Drosophila intestinal stem cells
    Divya Mathur
    Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA
    Science 327:210-3. 2010
    ..Our results demonstrate a paradigm for stem cell-niche biology, where progenitors generate transient niches that determine stem cell fate and may give insights into stem cell specification in other tissues...
  51. pmc Tissue damage-induced intestinal stem cell division in Drosophila
    Alla Amcheslavsky
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell Stem Cell 4:49-61. 2009
    ..By using this newly established system of intestinal stem cell proliferation and tissue regeneration, we find that the insulin receptor signaling pathway is required for intestinal stem cell division...
  52. ncbi In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain
    Jean Christophe Bambou
    INSERM, EMI 0212, 156 rue de Vaugirard, 75015 Paris, France
    J Biol Chem 279:42984-92. 2004
    ..The response of enterocytes in situ was then assessed using murine ileal biopsies mounted in Ussing chambers. Commensal E...
  53. ncbi Apc deficiency is associated with increased Egfr activity in the intestinal enterocytes and adenomas of C57BL/6J-Min/+ mice
    Amy E Moran
    Department of Surgery, Weill College of Medicine of Cornell University, and Strang Cancer Prevention Center, New York, New York 10021, USA
    J Biol Chem 279:43261-72. 2004
    ..analyses showed that Egfr activity was highest in the tumors, and also up-regulated in Min/+ relative to WT enterocytes. Expression of ubiquitylated Egfr (Egfr-Ub) was increased in Min/+ enterocytes and tumors...
  54. ncbi Endotoxin inhibits intestinal epithelial restitution through activation of Rho-GTPase and increased focal adhesions
    Selma Cetin
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:24592-600. 2004
    ..Intestinal mucosal defects are repaired by the process of intestinal restitution, during which enterocytes migrate from healthy areas to sites of injury...
  55. pmc Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes
    Nicholas Douris
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Curr Biol 21:1347-55. 2011
    ..Mice lacking nocturnin (Noc(-/-) mice) are resistant to diet-induced obesity and hepatic steatosis yet are not hyperactive or hypophagic...
  56. ncbi Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages
    Soichiro Ogaki
    Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo, Kumamoto, Japan
    Stem Cells 31:1086-96. 2013
    ..Upon prolonged culture on feeder cells, all four intestinal differentiated cell types, the absorptive enterocytes and three types of secretory cells (goblet cells, enteroendocrine cells, and Paneth cells), were efficiently ..
  57. ncbi Plasma citrulline: A marker of enterocyte mass in villous atrophy-associated small bowel disease
    Pascal Crenn
    Department of Hepagastroenterology and Nutrion Support, Hopital Lariboisiere, Paris, France
    Gastroenterology 124:1210-9. 2003
    Plasma citrulline, a nonprotein amino acid produced by enterocytes, was suggested as a marker of remnant enterocyte mass in patients with short bowel...
  58. ncbi Transglutaminase 2 in the enterocytes is coeliac specific and gluten dependent
    F Biagi
    1st Department of Internal Medicine, IRCCS Policlinico San Matteo, University of Pavia, Piazzale Golgi, 19, 27100 Pavia, Italy
    Dig Liver Dis 38:652-8. 2006
    Tissue transglutaminase, the coeliac autoantigen, was shown to localise in the enterocytes of coeliac patients and controls. It was speculated that surface tissue transglutaminase has a role in the pathogenesis of coeliac disease.
  59. pmc Probiotics prevent necrotizing enterocolitis by modulating enterocyte genes that regulate innate immune-mediated inflammation
    Kriston Ganguli
    Mucosal Immunology Laboratory, Division of Pediatric Gastroenterology, Massachusetts General Hospital for Children, 114 16th St 114 3503, Charlestown, MA 02192 4404, USA
    Am J Physiol Gastrointest Liver Physiol 304:G132-41. 2013
    ..After exposure to probiotic conditioned media (PCM), immature human enterocytes, immature human intestinal xenografts, and primary enterocyte cultures of NEC tissue (NEC-IEC) were assayed for ..
  60. pmc LPS-binding protein enables intestinal epithelial restitution despite LPS exposure
    Juli M Richter
    Center for Perinatal Research, The Research Institute at Nationwide Children s Hospital, Nationwide Children s Hospital, Columbus, OH 43205, USA
    J Pediatr Gastroenterol Nutr 54:639-44. 2012
    ..Lipopolysaccharide (LPS)-binding protein (LBP) is secreted by enterocytes in response to inflammatory stimuli and has concentration-dependent effects...
  61. ncbi Mycobacterium avium subsp. paratuberculosis invades through M cells and enterocytes across ileal and jejunal mucosa of lambs
    Duraisamy Ponnusamy
    Division of Veterinary Pathology, Indian Veterinary Research Institute, Izatnagar 243122, UP, India
    Res Vet Sci 94:306-12. 2013
    ..All these parameters revealed that Map invaded through M cells and the enterocytes and bacterial translocation across M cells was greater than the enterocytes...
  62. ncbi Proteasome inhibition of pathologic shedding of enterocytes to defend barrier function requires X-linked inhibitor of apoptosis protein and nuclear factor κB
    Derek M Foster
    Department of Population Health, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27607, USA
    Gastroenterology 143:133-44.e4. 2012
    ..We used a piglet model of Cryptosporidium parvum infection to determine how elimination of infected enterocytes is balanced with the need to maintain barrier function.
  63. pmc MK2-dependent p38b signalling protects Drosophila hindgut enterocytes against JNK-induced apoptosis under chronic stress
    Gerhard Seisenbacher
    Institute of Molecular Systems Biology, Swiss Federal Institute of Technology Zurich ETH Zurich, Zurich, Switzerland
    PLoS Genet 7:e1002168. 2011
    ..of the gut epithelium by intestinal stem cells contributes to gut homeostasis, but how the differentiated enterocytes are protected against stressors is less well understood...
  64. pmc LRH-1-mediated glucocorticoid synthesis in enterocytes protects against inflammatory bowel disease
    Agnes Coste
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS, INSERM, Universite Louis Pasteur, Illkirch, and Service des Maladies de l Appareil Digestif et de la Nutrition, Hopital Huriez, Lille, France
    Proc Natl Acad Sci U S A 104:13098-103. 2007
    ..These findings underscore the importance of LRH-1 in the control of intestinal inflammation and the pathogenesis of inflammatory bowel disease...
  65. ncbi Sugar sensing by enterocytes combines polarity, membrane bound detectors and sugar metabolism
    Maude Le Gall
    INSERM, UMR S 872, Centre de Recherche des Cordeliers, Paris, France
    J Cell Physiol 213:834-43. 2007
    ..By providing sugar metabolites, sugar transporters, including GLUT2, fuelled a sensing pathway. In Caco2/TC7 enterocytes, we could disconnect the sensing triggered by detector from that produced by metabolism, and found that GLUT2 ..
  66. ncbi Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation
    Jaroslav Pejchal
    Center of Advanced Studies, University of Defence, Hradec Kralove, Czech Republic
    Int J Radiat Biol 88:348-58. 2012
    ..the p38 mitogen-activated protein kinase (p38) phosphorylation and transforming growth factor beta 1 (TGF-β1) expression in rat colon enterocytes after irradiation and their contribution to pathology of intestinal radiation disease.
  67. pmc Laser microdissection coupled with RNA-seq analysis of porcine enterocytes infected with an obligate intracellular pathogen (Lawsonia intracellularis)
    Fabio A Vannucci
    Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    BMC Genomics 14:421. 2013
    ..We used laser capture microdissection coupled with RNA-seq technology to characterize the transcriptional responses of infected enterocytes and the host-pathogen interaction.
  68. ncbi Elevated expression of iNOS mRNA and protein in coeliac disease
    Ian Daniels
    David Evans Medical Research Centre, City Hospital, Nottingham, NG5 1PB, UK
    Clin Chim Acta 356:134-42. 2005
    ..The role of nitric oxide synthase (NOS) in the pathophysiology of coeliac disease (CD) was investigated...
  69. ncbi A direct role for NKG2D/MICA interaction in villous atrophy during celiac disease
    Sophie Hue
    Equipe Avenir Inserm, Hopital Necker Enfants Malades, 75015 Paris, France
    Immunity 21:367-77. 2004
    ..Villous atrophy in Celiac disease might thus be ascribed to an IEL-mediated damage to enterocytes involving NKG2D/MICA interaction after gliadin-induced expression of MICA on gut epithelium...
  70. ncbi Lipopolysaccharide induces cyclooxygenase-2 in intestinal epithelium via a noncanonical p38 MAPK pathway
    Anatoly V Grishin
    Division of Pediatric Surgery, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA
    J Immunol 176:580-8. 2006
    ..LPS-induced production of inflammatory factors in immature enterocytes may be a factor in NEC...
  71. ncbi Construction and use of a Trichinella spiralis phage display library to identify the interactions between parasite and host enterocytes
    Hui jun Ren
    Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, 450052, People s Republic of China
    Parasitol Res 112:1857-63. 2013
    ..It is speculated that the molecular interactions between the parasite and host enterocytes may mediate the recognition and invasion of IECs by T. spiralis...
  72. pmc Secretory IgA mediates retrotranscytosis of intact gliadin peptides via the transferrin receptor in celiac disease
    Tamara Matysiak-Budnik
    Institut National de la Sante et de la Recherche Medicale INSERM, U793, Paris 75730, Cedex 15, France
    J Exp Med 205:143-54. 2008
    ..Our findings strongly implicate CD71 in the pathogenesis of CD...
  73. ncbi Role of Caco-2 cell monolayers in prediction of intestinal drug absorption
    Pranav Shah
    Department of Pharmacy, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Post Box 51, Kalabhavan, Vadodara 390 001, India
    Biotechnol Prog 22:186-98. 2006
    ..The role of Caco-2 cell monolayers in studying the effectiveness, involved mechanism and toxicity of various excipients for drug absorption promotion has also been discussed...
  74. pmc Discovery of a cytosolic/soluble ferroxidase in rodent enterocytes
    Perungavur N Ranganathan
    Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, USA
    Proc Natl Acad Sci U S A 109:3564-9. 2012
    Hephaestin (Heph), a membrane-bound multicopper ferroxidase (FOX) expressed in duodenal enterocytes, is required for optimal iron absorption...
  75. pmc Gut triglyceride production
    Xiaoyue Pan
    Department of Cell Biology, SUNY Downstate Medical Center, 450 Clarkson Ave, Brooklyn, NY 11203, USA
    Biochim Biophys Acta 1821:727-35. 2012
    ..hydrolyzed in the intestinal lumen to free fatty acids (FFA) and monoacylglycerols (MAG), which are taken up by enterocytes from their apical side, transported to the endoplasmic reticulum (ER) and resynthesized into TAG...
  76. ncbi Interferon-gamma inhibits intestinal restitution by preventing gap junction communication between enterocytes
    Cynthia L Leaphart
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Gastroenterology 132:2395-411. 2007
    ..Because enterocytes migrate together, mucosal healing may require interenterocyte communication via connexin 43-mediated gap ..
  77. ncbi Activation of peroxisome proliferator-activated receptor-α (PPARα) suppresses postprandial lipidemia through fatty acid oxidation in enterocytes
    Rino Kimura
    Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Biochem Biophys Res Commun 410:1-6. 2011
    ..In this study, we examined the effects of PPARα activation in enterocytes on lipid secretion and postprandial lipidemia...
  78. ncbi The role of oxygen-free radical in the apoptosis of enterocytes and bacterial translocation in abdominal compartment syndrome
    Guanwen Gong
    Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province, PR China
    Free Radic Res 43:470-7. 2009
    ..The purpose of this study was to study the impact of intra-abdominal hypertension (IAH) on the intestine...
  79. pmc Fenofibrate, a peroxisome proliferator-activated receptor α agonist, alters triglyceride metabolism in enterocytes of mice
    Aki Uchida
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
    Biochim Biophys Acta 1811:170-6. 2011
    ..These results suggest that the effects of fenofibrate on the small intestine play a critical role in the hypotriglyceridemic effects of fenofibrate...
  80. pmc Arginine stimulates intestinal cell migration through a focal adhesion kinase dependent mechanism
    J M Rhoads
    Department of Pediatrics, and Center in Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, North Carolina, USA
    Gut 53:514-22. 2004
    ..L-Arginine is a nutritional supplement that may be useful for promoting intestinal repair. Arginine is metabolised by the oxidative deiminase pathway to form nitric oxide (NO) and by the arginase pathway to yield ornithine and polyamines...
  81. ncbi Evidence for intestinal chloride secretion
    Michael Murek
    Department of Surgery, Yale School of Medicine, New Haven, CT 06511, USA
    Exp Physiol 95:471-8. 2010
    ..This article reviews the widely accepted model of intestinal chloride secretion with an emphasis on the molecular players involved in this tightly regulated process...
  82. pmc Primary murine small intestinal epithelial cells, maintained in long-term culture, are susceptible to rotavirus infection
    K K Macartney
    Section of Infectious Diseases, The Children s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA
    J Virol 74:5597-603. 2000
    ..Cultured IEC had the morphologic and functional characteristics of immature enterocytes, notably sustained expression of cytokeratin and alkaline phosphatase...
  83. ncbi Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic {beta}-cells
    David M Hudson
    Centre for Blood Research and Department of Biochemistry, University of British Columbia, Vancouver, Canada
    Am J Physiol Gastrointest Liver Physiol 298:G425-32. 2010
    ..studies in rat, mouse, and human gut tissues localized Hp to the basolateral membranes of the duodenal enterocytes where the Hp was predicted to aid in the transfer of Fe(III) to transferrin in the blood...
  84. ncbi Insulin down-regulates the Na+/K+ ATPase in enterocytes but increases intestinal glucose absorption
    Maya F Serhan
    Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon
    Gen Comp Endocrinol 167:228-33. 2010
    ..Considering the inhibitory effect of the hormone on the Na(+)/K(+) ATPase, it was concluded that insulin acts by increasing the number of glucose transporters, a hypothesis that was confirmed by Western blot analysis...
  85. ncbi Ctr1 and its role in body copper homeostasis
    Paul A Sharp
    Centre for Nutrition and Food Safety, School of Biomedical and Life Sciences, University of Surrey, Guildford GU2 7XH, UK
    Int J Biochem Cell Biol 35:288-91. 2003
    ..However, its role in other tissues remains elusive...
  86. ncbi NF-kappaB-mediated expression of MAPK phosphatase-1 is an early step in desensitization to TLR ligands in enterocytes
    J Wang
    Division of Pediatric Surgery, Childrens Hospital Los Angeles, Los Angeles, California, USA
    Mucosal Immunol 3:523-34. 2010
    Toll-like receptor (TLR) signaling in naive enterocytes is rapidly inhibited, leading to the establishment of tolerance...
  87. ncbi Mechanisms involved in vitamin E transport by primary enterocytes and in vivo absorption
    Kamran Anwar
    Molecular and Cellular Biology Program, School of Graduate Studies, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
    J Lipid Res 48:2028-38. 2007
    ..Here, we used primary enterocytes and rodents to identify in vivo vitamin E absorption pathways...
  88. ncbi Involvement of CD36 and intestinal alkaline phosphatases in fatty acid transport in enterocytes, and the response to a high-fat diet
    Matthew D Lynes
    Department of Biology, Boston University, Boston, MA 02215, United States
    Life Sci 88:384-91. 2011
    ..A model is proposed in which two key proteins, CD36 and the enzyme intestinal alkaline phosphatase, work in a coordinated manner to optimize fatty acid transport across enterocytes in mice.
  89. ncbi Evidence for multiple complementary pathways for efficient cholesterol absorption in mice
    Jahangir Iqbal
    Department of Anatomy and Cell Biology, and Pediatrics, SUNY Downstate Medical Center, Brooklyn, NY, USA
    J Lipid Res 46:1491-501. 2005
    ..Cholesterol secretion by the HDLs, but not by the apoB pathway, was significantly reduced in primary enterocytes isolated from chow- and cholesterol-fed apoA-I(-/-) mice...
  90. pmc MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts
    Anita Balakrishnan
    Department of Surgery, Brigham and Women s Hospital, Boston, MA 02115, USA
    Exp Cell Res 316:3512-21. 2010
    ..The regulatory mechanisms behind these rhythms remain largely unknown. We hypothesized that microRNAs are involved in mediating these rhythms, and studied the role of microRNAs specifically in modulating intestinal proliferation...
  91. pmc Nucleotide-binding oligomerization domain-2 inhibits toll-like receptor-4 signaling in the intestinal epithelium
    Ward M Richardson
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Gastroenterology 139:904-17, 917.e1-6. 2010
    ..enterocolitis (NEC) remain incompletely understood, although Toll-like receptor-4 (TLR4) signaling in enterocytes plays a major role...
  92. ncbi Effects of HIV-1 Tat protein on ion secretion and on cell proliferation in human intestinal epithelial cells
    Roberto Berni Canani
    Department of Pediatrics, University of Naples Federico II, Italy
    Gastroenterology 124:368-76. 2003
    ..The HIV-1 transactivating factor protein (Tat) is a key factor in the pathogenesis of acquired immunodeficiency syndrome. We investigated whether Tat could directly induce ion secretion and cell damage in enterocytes.
  93. pmc Lactobacillus acidophilus induces a slow but more sustained chemokine and cytokine response in naïve foetal enterocytes compared to commensal Escherichia coli
    Louise H Zeuthen
    Department of Systems Biology, Technical University of Denmark, Center for Biological Sequence Analysis, 2800 Kgs, Lyngby, Denmark
    BMC Immunol 11:2. 2010
    ..Facultative anaerobic bacteria are the first to colonise the infant gut, and the impact of these bacteria on intestinal epithelial cells (IEC) may be determinant for how the immune system subsequently tolerates gut bacteria...
  94. ncbi The orchestration of body iron intake: how and where do enterocytes receive their cues?
    David M Frazer
    Joint Clinical Sciences Program, Queensland Institute of Medical Research and University of Queensland, PO Royal Brisbane Hospital, Brisbane, Queensland 4029, Australia
    Blood Cells Mol Dis 30:288-97. 2003
    ..In particular, this analysis suggests that signals to alter absorption exert a direct effect on mature enterocytes rather than influencing the intestinal crypt cells...
  95. ncbi L-Glutamine or L-alanyl-L-glutamine prevents oxidant- or endotoxin-induced death of neonatal enterocytes
    Tony E Haynes
    Department of Animal Science, Texas A and M University, College Station, TX 77843, USA
    Amino Acids 37:131-42. 2009
    ..L-glutamine (Gln) or L-alanyl-L-glutamine (Ala-Gln) prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Enterocytes of neonatal pigs rapidly hydrolyzed Ala-Gln and utilized Gln...
  96. ncbi Zebrafish cdx1b regulates differentiation of various intestinal cell lineages
    Yi Hua Chen
    Institute of Cellular and Organismic Biology formerly Institute of Zoology, Academia Sinica, Nankang, Taipei, Taiwan, ROC
    Dev Dyn 238:1021-32. 2009
    ..Decreased PepT1 expression was detected in enterocytes of intestines in cdx1b morphants from 80 to 102 hr of development...
  97. pmc Tight junction proteins claudin-2 and -12 are critical for vitamin D-dependent Ca2+ absorption between enterocytes
    Hiroki Fujita
    Departments of Pathology and Orthopedic Surgery, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Mol Biol Cell 19:1912-21. 2008
    ..We also provide evidence showing that expression of claudins-2 and -12 is up-regulated in enterocytes in vitro and in vivo by 1alpha,25(OH)(2)D(3) through the vitamin D receptor...
  98. ncbi Development and physiological regulation of intestinal lipid absorption. I. Development of intestinal lipid absorption: cellular events in chylomicron assembly and secretion
    Dennis D Black
    Children s Foundation Research Center of Memphis, Le Bonheur Children s Medical Center, 50 N Dunlap Street, Memphis, TN 38103, USA
    Am J Physiol Gastrointest Liver Physiol 293:G519-24. 2007
    ..These proteins are regulated in a manner to maximize the lipid absorptive capacity of the newborn intestine...
  99. ncbi Gut expression and regulation of FAT/CD36: possible role in fatty acid transport in rat enterocytes
    M Chen
    Department of Surgery, University of Alabama at Birmingham, 35233, USA
    Am J Physiol Endocrinol Metab 281:E916-23. 2001
    ..These findings suggest that FAT/CD36 plays a role in the uptake of LCFA by small intestinal enterocytes. This may have important implications in understanding fatty acid absorption in human physiological and ..
  100. ncbi CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine
    Fatiha Nassir
    Department of Medicine, Division of Nutritional Science, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:19493-501. 2007
    ..protein that facilitates fatty acid uptake, is highly expressed in the intestine on the luminal surface of enterocytes. Cd36 null (Cd36(-/-)) mice exhibit impaired chylomicron secretion but no overall lipid absorption defect...
  101. ncbi Enterocyte differentiation marker intestinal alkaline phosphatase is a target gene of the gut-enriched Kruppel-like factor
    Brian F Hinnebusch
    Deptartment of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Gastrointest Liver Physiol 286:G23-30. 2004
    ..We have identified the enterocyte differentiation marker IAP as a KLF4 target gene. IAP transactivation by KLF4 is likely mediated through a critical region located within the proximal IAP promoter region...

Research Grants73

  1. Lipid Droplet Metabolism
    Fredric B Kraemer; Fiscal Year: 2013
    ..but can be found in many, if not most, tissues and cells, including liver, skeletal muscle, cardiac muscle, enterocytes, and leukocytes, under certain physiological and pathophysiological conditions where the LDs generally serve as ..
  2. Vitamin A adjuvant to enhance gut immunity and rotavirus vaccines in neonates
    Linda J Saif; Fiscal Year: 2010
    ..To test this, we will use attenuated RV that replicates in enterocytes, (increase proinflammatory, decrease TGF[unreadable] cytokines) as oral vaccine with VitA adjuvant to enhance ..
  3. Modifiers of hepcidin expression as new therapies for iron overload
    PAULA GOODMAN FRAENKEL; Fiscal Year: 2013
    ..binds the iron exporter ferroportin1, causing internalization of both proteins and reduced iron release from enterocytes to the circulation and from macrophages to other tissues...
  4. Stem Cell Based Therapy For Radiation Induced Gastrointestinal Syndrome
    Chandan Guha; Fiscal Year: 2010
    ..IR induces apoptosis of ISCs, crypt endothelial cells and enterocytes within hours...
  5. MOLECULAR REGULATION OF ILEAL/RENAL BILE ACID TRANSPORT
    Benjamin L Shneider; Fiscal Year: 2012
    ..We have recently shown in enterocytes and cholangiocytes that AP-1 and FXR-mediated bile acid regulatory pathways are also influenced by FGF-19 via ..
  6. Breakthroughs to advance the in vitro propagation of human noroviruses
    Qiuhong Wang; Fiscal Year: 2010
    ..as in vivo and in vitro MNV targets;3) Histo-blood group antigens as potential receptors on human and pig enterocytes that influence susceptibility to HuNoV;4) Use of the dominant HuNoV outbreak strain (GII...
  7. Quorum sensing regulation of EHEC virulence genes
    Vanessa Sperandio; Fiscal Year: 2013
    ..traits in EHEC ensuring their timely expression, so EHEC can swim through the mucus layer, form AE lesions on enterocytes, and express and release Shiga toxin...
  8. Molecular Mechanism of cell-cell spread of Listeria in polarized epithelial cells
    KEITH PATRICK IRETON; Fiscal Year: 2013
    ..Cell-cell spread of Listeria occurs in a variety of host cell types, including intestinal epithelial cells (enterocytes)...
  9. Regulation of Organized Intestinal Lymphoid Tissues by TRANCE/RANKL
    Ifor R Williams; Fiscal Year: 2012
    ..effects on intestinal DC and macrophages influence the effects of stromal cell RANKL on RANK-expressing enterocytes. Mechanistic insights into how the RANKL-RANK pathway supports pathways of antigen handling by the gut immune ..
  10. Intestinal Triacylglycerol Metabolism and Energy Balance
    Chi Liang Eric Yen; Fiscal Year: 2013
    ..Thus, we hypothesize that MGAT2 coordinates the uptake and processing of lipid for chylomicron formation in enterocytes and modulates the secretion of GIP and GLP1 from enteroendocrine cells...
  11. The Transport of Nutritional Heme in Animal Development
    Iqbal Hamza; Fiscal Year: 2013
    ..source of bioavailable iron, but the genes and pathways responsible for heme transport and utilization in human enterocytes remain elusive. In humans, greater than 60% of total body iron is present as heme in hemoglobin...
  12. Molecular Mechanisms of Intestinal Metal Ion Transport During Iron Deficiency
    James F Collins; Fiscal Year: 2013
    ..We made the novel observation that copper-related processes are activated by iron deprivation of rodents. In enterocytes, a copper transporting ATPase (Atp7a) and a copper-binding protein (metallothionein) were upregulated in the ..
  13. Gut mucosal mast cells are activated by fat absorption: physiology and mechanism
    Patrick Tso; Fiscal Year: 2013
    ..Specific Aim 3. We will test our hypothesis that fatty acid or the processing of CMs by the enterocytes results in the release of factor/factors that cause the activation of the MMCs (e.g. NF-kB response)...
  14. Genes required for lipid processing and digestive organ function in larval zebraf
    Steven A Farber; Fiscal Year: 2011
    ..While it is well known that intestinal enterocytes are the main absorptive cell that can take in large amounts of dietary lipid and export it in the form of ..
  15. CD36 and Intestinal Fat Absorption
    Nada A Abumrad; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The scavenger receptor CD36 is highly expressed on the apical side of enterocytes of the proximal intestine and on endothelial and immune cells throughout the organ...
  16. The role of caveolin 1 in intestinal lipid processing and transport
    JESSICA OTIS; Fiscal Year: 2013
    ..studies suggest that caveolae plasma membrane expression may be asymmetric in polarized cell types such as enterocytes, but data have been conflicting...
  17. CAT1920: A Small Molecule Therapeutic for the Treatment of IBD
    MICHAEL ROBERT JIROUSEK; Fiscal Year: 2010
    ..the effects of CAT1920 in intestinal epithelial cells, and we will confirm the modulation of this gene panel in enterocytes from CAT1920 treated animals...
  18. Activation of Host-Probiotic Communication by Prebiotic Milk Oligosaccharides
    Helen E Raybould; Fiscal Year: 2012
    ..oligosaccharides and bifidobacterial species in binding and activation of two types of GI epithelial cells, enterocytes and EC cells and to determine the downstream benefits of this interaction in terms of improved regulation of ..
  19. Functions of MRP2 and MRP3 in Drug Disposition
    James M Gallo; Fiscal Year: 2011
    ..MRP3, which is able to transport etoposide and methotrexate, is localized on the basolateral surfaces of gut enterocytes, and is induced at basolateral surfaces of hepatocytes during conditions of liver dysfunction (cholestasis) in ..
  20. Intestinal Calcium Absorption: Molecular Mechanism
    James C Fleet; Fiscal Year: 2012
    ..of Ca absorption with aging, (3) To determine the factors controlling vitamin D-mediated gene activation in enterocytes. We will conduct cell and animal studies to determine the active role RXR[unreadable] has in VDR mediated gene ..
  21. Functional Analysis of MGAT Enzymes
    Robert V Farese; Fiscal Year: 2010
    ..and fatty acyl CoA, is crucial for the absorption of dietary TG and for chylomicron assembly and secretion in enterocytes. We also hypothesize that MGAT2 contributes to reassembly of TG for hepatic storage or secretion and to the ..
  22. Enteric Virus Calcium Channel Inhibitors
    Joseph M Hyser; Fiscal Year: 2013
    ..One mechanism for the destruction of RV-infected enterocytes is the dramatic elevation in cytoplasmic calcium, which is triggered by the rotavirus nonstructural protein 4 (..
  23. Regulation of TLR4 Signaling in Enterocytes in Necrotizing Enterocolitis
    Amin Afrazi; Fiscal Year: 2013
    ..Activation of TLR4 on enterocytes by LPS leads to an increase in enterocyte apoptosis and villus loss, as well as reduced intestinal repair by ..
  24. The Role of MTP in Lipid Droplet Formation in Adipocytes
    Larry L Swift; Fiscal Year: 2013
    ..protein (MTP), a protein essential for the assembly of triglyceride-rich lipoproteins within hepatocytes and enterocytes, is expressed in adipocytes of mice, rats, and humans...
  25. Role of taste signaling elements in enteroendocrine cells
    Robert F Margolskee; Fiscal Year: 2013
    ..their expression of taste signaling elements and elicits upregulation of sugar and fatty acid transporters in enterocytes;3...
  26. Cholesterol Uptake by Cryptosporidium
    Isabelle Coppens; Fiscal Year: 2010
    ..Cryptosporidium infection begins with microbial invasion of small intestinal epithelial cells (enterocytes), in which the parasite creates a peculiar niche that sequesters itself from the intestinal lumen and the host ..
  27. Sugar Regulation of EHEC virulence
    Vanessa Sperandio; Fiscal Year: 2013
    ..effacement (LEE) pathogenicity island necessary for the formation of attaching and effacing (AE) lesions on enterocytes. Glycolytic environments inhibit the expression of the LEE genes...
  28. S1P Lyase in colon cancer
    Julie D Saba; Fiscal Year: 2013
    ..However, sphingosine taken up by enterocytes can be phosphorylated by the oncogenic enzyme SphK1, generating sphingosine-1-phosphate (S1P), a mitogenic ..
  29. Inhibition of Toxin Translocation Can Reverse Cholera and ETEC-Mediated Diarrhea
    KENNETH R TETER; Fiscal Year: 2013
    ..It is believed that recovery from cholera and ETEC-mediated diarrhea can only occur after intoxicated enterocytes, which have a 3-5 day lifespan, are sloughed from the intestinal epithelium...
  30. Epithelial Glycoconjugates as Barriers against Enteric Infections
    Nicholas J Mantis; Fiscal Year: 2013
    ..commonly use lectin-like adhesins to adhere to host glycoproteins and glycolipids expressed on small intestinal enterocytes and/or M cells...
  31. Spatiotemporal Regulation of Intestinal Gene Expression
    Eric Sibley; Fiscal Year: 2013
    ..associated with lactase persistence function to regulate lactase gene transcription in adult human enterocytes via differential interaction with specific nuclear protein transcription factors...
  32. Role of Rab proteins in hSVCT1 cell biology in intestinal epithelial cells
    Veedamali S Subramanian; Fiscal Year: 2010
    ..aspects of hSVCT1 with specific regards to membrane targeting/localization, trafficking and function in human enterocytes. Recent studies have shown that the alteration in membrane targeting and trafficking events may affect the ..
  33. Chemical Genetics of Iron Transport
    Marianne Wessling-Resnick; Fiscal Year: 2013
    ..by DMT1, a ferrous iron transporter that functions in dietary iron absorption across the apical membrane of enterocytes and after iron delivery into endosomes by the transferrin-mediated pathway...
  34. Molecular Mechanisms of Campylobacter Jejuni-induced Pathogenesis
    Christian Jobin; Fiscal Year: 2011
    ..jejuni. Our goal is to selectively delete RelA (NF?B subunit) in enterocytes and myeloid cells of IL-10-/- mice to address the function of NF?B signaling in the host response to C. jejuni...
  35. Functional Relevance of CFTR Trafficking in Vivo to Intestinal Disease
    Nadia A Ameen; Fiscal Year: 2013
    ..Unlike other epithelia that express CFTR only on the BBM, enterocytes express CFTR both on the BBM and subapical endosomes...
  36. BARRIER FUNCTION OF THE GI TRACT IN HEALTH AND DISEASE
    W Allan Walker; Fiscal Year: 2013
    This Program Project renewal application continues to have a narrow focus on the role of enterocytes in mucosal barrier function at the interface between microbial and enterotoxin-mediated stimuli and immune effector responses...
  37. FUNCTION OF THE HEMOCHROMATOSIS PROTEIN
    CAROLINE ENNS; Fiscal Year: 2009
    ..In the early stages of the disease, Kupffer cells in the liver and enterocytes in the intestine cells are iron depleted and have low intracellular ferritin levels, whereas hepatocytes in the ..
  38. Hepcidin Replacement Therapy for Iron Overload Disorders
    James W Larrick; Fiscal Year: 2013
    ..iron metabolism by triggering degradation of ferroportin, an iron-transport protein localized on absorptive enterocytes as well as hepatocytes and macrophages...
  39. Live Oral Listeria Vaccine Vector
    PAUL EDWIN ORNDORFF; Fiscal Year: 2010
    ..revealed its potential value as a vaccine platform: First, the mutant made minute plaques on cultured mouse enterocytes, indicating that it was not attenuated to the point where it would be nonimmunogenic...
  40. MicroRNAs in small intestinal epithelial cell proliferation and differentiation
    ANNE MATHEA HUTSON; Fiscal Year: 2012
    ..from the crypts, the site of cell proliferation, to the villi, where terminally differentiated absorptive enterocytes and secretory cells function...
  41. Understanding the Role of N-WASP in the Intestinal Epithelium
    John Garber; Fiscal Year: 2010
    ..than wild type (WT) controls;they have grossly normal mucosal architecture, with preserved ability of the enterocytes to differentiate into all intestinal cell lineages, and there is no spontaneous inflammation...
  42. Nutrient regulation of stem cell mediated intestinal renewal in Drosophila
    LUCY ERIN O'BRIEN; Fiscal Year: 2013
    ..Aim 3 will investigate the role of local cellular interactions by determining how enterocytes act through tissue structure to control the proliferation of nearby stem cells, culminating in a genetic screen ..
  43. Tolerance to TLR Ligands in the Intestinal Epithelium
    Anatoly V Grishin; Fiscal Year: 2010
    ..Intestinal epithelial cells (enterocytes) express pathogen pattern recognition Toll-like receptors (TLR), and produce inflammatory factors in response ..
  44. Immunogenicity of an Attenuated Listeria monocytogenes Bacteriophage Resistant Mu
    PAUL EDWIN ORNDORFF; Fiscal Year: 2010
    ..are attenuated when inoculated orally into female A/J mice and show impaired replication in cultured mouse enterocytes. One of these mutants, containing a Tn917 insertion in the glcV gene, has been extensively characterized...
  45. Impacts of Molecular Oxygen on the Structure and Function of the Intestinal Micro
    Vincent B Young; Fiscal Year: 2013
    ..of mucosal immunity and production of short-chain fatty acids - the preferred energy source of colonic enterocytes and a potent immunomodulatory signal...
  46. The iron modulatory function of prion protein and prion disorders
    Neena Singh; Fiscal Year: 2013
    ..polarized Caco-2 cells transfected to express PrPC or mutant PrP forms will be used as models of absorptive enterocytes to understand the mechanism of iron transport by PrPC...
  47. Role of transglutaminase 2 in celiac sprue
    Chaitan Khosla; Fiscal Year: 2013
    ..In sub-Aim A, we will use cultured enterocytes and fibroblasts to identify such substrates. Sub-Aim 2 seeks to validate their identity in the mouse intestine...
  48. Signaling in digestive epithelial development and homeostasis
    Ramesh A Shivdasani; Fiscal Year: 2013
    ..isolate the earliest stem-cell progeny, bipotential progenitors able to differentiate into secretory cells or enterocytes. We will study the epigenetic basis and Atoh1 dependencies for their subsequent commitment to a single fate...
  49. Dynamic regulation of epithelial cell survival by enteropathogenic E. coli
    V K Viswanathan; Fiscal Year: 2013
    ..We hypothesize that EspZ interacts with host proteins to suppress premature death of infected enterocytes and, thereby, plays a key role in intestinal colonization by A/E pathogens...
  50. EFFECT OF COLOSTRUM ON GUT MATURATION AND HOST DEFENSE
    W Allan Walker; Fiscal Year: 2013
    ..we have begun to examine DNA microarrays of laser capture microdisection epithelial RNA from immature vs mature enterocytes, confirmed by qRT-PCR...
  51. Physiological role of GLP-2 receptor in the mouse intestinal growth and function
    Xinfu Guan; Fiscal Year: 2009
    ..Dual localization of the GLP-2R protein to enteric neurons and enteroendocrine cells, but not enterocytes, suggests that the GLP-2-mediated trophic actions on the epithelium are mediated indirectly through an ..
  52. Bacterially-Secreted GLP-1 Induced Reprogramming of Intestinal Cells as a Treatme
    John C March; Fiscal Year: 2012
    ..We have developed a novel method for local and targeted delivery of GLP-1 peptide to adult enterocytes in situ, using genetically-modified commensal bacteria...
  53. A Mouse Model of Acrodermatitis Enteropathica
    Glen K Andrews; Fiscal Year: 2012
    ..opposing functions is based on our finding that they localize to opposite membranes of polarized intestinal enterocytes and visceral endoderm cells, cell-types critical for proper zinc homeostasis, and they show opposite responses ..
  54. Cellular Efflux and Metabolism of Protease Inhibitors
    Ashim Mitra; Fiscal Year: 2004
    ..the applicant): Cytochrome P450 3A4 (CYP3A4), most abundantly presently both in the liver and upper intestinal enterocytes, limits the systemic bioavailability of anti-HIV agents...
  55. Myosin Functions in the Enterocyte
    Mark S Mooseker; Fiscal Year: 2010
    ..There are marked compositional and structural defects in the BB membrane and underlying cytoskeleton in enterocytes of mice lacking Myola including the loss of Myo6 and Myole...
  56. SdiA regulation of EHEC virulence
    Vanessa Sperandio; Fiscal Year: 2013
    ..locus of enterocyte effacement (LEE) region, involved in intestinal adhesion, and formation of lesions on enterocytes named attaching and effacing (AE) lesions, as well as a functional SdiA transcription factor...
  57. The role of the mu-Opioid Receptor in Inflammatory Bowel Disease Pathology and Th
    Jason R Goldsmith; Fiscal Year: 2013
    ..of STAT3 phosphorylation, and other prelminary data shows the MOR agonist induces STAT3 activation in cultured enterocytes. Consequently, we hypothesize that the activation of MOR signaling plays a beneficial role in DSS-induced ..
  58. Physiological Role of NPC1L1 in Lipid Transport and Metabolism
    David Y Hui; Fiscal Year: 2010
    ..However, whether NPC1L1 is a membrane bound protein serving as a transporter for cholesterol uptake by enterocytes or an intracellular protein responsible for cholesterol trafficking from the apical plasma membrane to ..
  59. Genes regulating M cell differentiation
    David D Lo; Fiscal Year: 2013
    ..Expression of Jagged-1 by the established M cells may also inhibit generation of M cells from adjacent enterocytes. The second step, functional maturation of M cells, appears to be dependent on interactions between M cells and ..
  60. Expression of Simiam Virus 40 T Antigens in Intestine
    James M Pipas; Fiscal Year: 2013
    ..In this application we propose to study the effects of T antigen expression on the growth-arrested enterocytes and continuously cycling progenitor cells of the mouse small intestine...
  61. Microbial Regulation of Macromolecule Absorption in the Zebrafish Intestine
    Jordan L Cocchiaro; Fiscal Year: 2012
    ..macromolecules (>600Da) can be transported across the intestinal epithelium through absorptive cells ("enterocytes")...
  62. Shiga toxin uptake mechanisms and intracellular action
    Olga Kovbasnjuk; Fiscal Year: 2010
    ..The EHEC-induced diseases required that Stx must be endocytosed by the enterocytes, cross the intestinal epithelial barrier and enter the bloodstream...
  63. C/EBP and IL-6 Production in Mucosa and Enterocytes
    Per Olof Hasselgren; Fiscal Year: 2006
    Sepsis and endotoxemia are associated with increased production of IL-6 in intestinal mucosa and enterocytes, a response that is augmented by the heat shock response...
  64. Syndecan and Bacterial Translocation in Shock and Trauma
    Carol Wells; Fiscal Year: 2006
    ..common finding in these patients is increased intestinal epithelial permeability, and experiments with cultured enterocytes have shown that bacterial adherence to and internalization by enterocytes is increased following opening of ..
  65. Intestinal Iron Transport in Iron Deficiency/Anemia
    James Collins; Fiscal Year: 2005
    ..proteins dcytb and DMT1 were consistently induced in anemia, while other genes involved in iron export from enterocytes such as ferroportin and hephaestin did not show changes in the iron deficient state...
  66. REGULATION OF ADENOSINE DEAMINASE IN SMALL INTESTINE
    Dan Wiginton; Fiscal Year: 2006
    ..After cell division and initiation of differentiation, immature cells begin an interesting bipolar migration. Enterocytes, goblet cells, and enteroendocrine cells migrate up along the villus, while Paneth cells migrate down into the ..
  67. EXOCYTIC INSERTION OF POTASSIUM CHANNELS IN MEMBRANES
    William Moran; Fiscal Year: 2001
    ..in apical membrane exocytosis and capacitance suggest that the increase in plasma membrane Gk in Aplysia enterocytes, a model nutrient absorbing epithelium, results from exocytic insertion of K channels into the apical membrane...
  68. Novel Anti-Obesity Drugs Targeting Fat Processing Pathway
    Henry Pelish; Fiscal Year: 2009
    ..These downstream steps occur in intestinal enterocytes, which process fats into triglyceride-rich particles for distribution in the body...
  69. Role of Myosin VI in CFTR Endocytosis in the Intestine
    Nadia Ameen; Fiscal Year: 2007
    ..in the native small intestine by insertion and trafficking of endosomal CFTR into the plasma membrane of enterocytes. While up-regulation of CFTR function on the surface of enterocytes is accepted to underly the pathogenesis of ..
  70. REGULATION OF AUTOREACTIVE T CELLS IN IL10 KNOCKOUT MICE
    Peter Ernst; Fiscal Year: 1999
    ..of IL-10 and interferon-gamma in the regulation of surface molecules expressed by cultured or freshly isolated enterocytes; a comparison of T cell reactivity to specific antigens presented by enterocytes treated with these cytokines ..
  71. EFFECT OF COLOSTRUM ON GUT MATURATION AND HOST DEFENSE
    W Walker; Fiscal Year: 1991
    ..factors" (hormones, lymphokines or epidermal growth factor) can modify the handling of antigens by neonatal enterocytes and stimulate a normal mucosal immune response to these antigens and whether these factors alter the ..
  72. INTESTINAL PEPTIDE HYDROLASES AND PEPTIDE TRANSPORT
    Young Kim; Fiscal Year: 2000
    ..focus on the peptidases and peptide transporters associated with the brush-border membrane of small intestinal enterocytes. These enzymes/transporters play an important role in the final phases of protein digestion and also serve as ..
  73. GASTROINTESTINAL PROTEINS--CELL AND MOLECULAR REGULATION
    Jeffrey Gordon; Fiscal Year: 2000
    ..chief and parietal cells of stomach, salivary acini and ducts, pancreatic exocrine cells) (projects 1-3); in enterocytes (projects 4-6), or in hepatocytes (project 6)...