cho cells

Summary

Summary: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

Top Publications

  1. ncbi Natural oligomers of the amyloid-beta protein specifically disrupt cognitive function
    James P Cleary
    Geriatric Research, Education and Clinical Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Nat Neurosci 8:79-84. 2005
  2. pmc The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
    Xun Xu
    BGI Shenzhen, Shenzhen, People s Republic of China
    Nat Biotechnol 29:735-41. 2011
  3. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
  4. pmc Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
    Colin K Choi
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 10:1039-50. 2008
  5. ncbi Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin
    Michael Bandell
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Neuron 41:849-57. 2004
  6. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
  7. ncbi Spatial control of EGF receptor activation by reversible dimerization on living cells
    Inhee Chung
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nature 464:783-7. 2010
  8. ncbi PD-L2 is a second ligand for PD-1 and inhibits T cell activation
    Y Latchman
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 2:261-8. 2001
  9. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
  10. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008

Detail Information

Publications316 found, 100 shown here

  1. ncbi Natural oligomers of the amyloid-beta protein specifically disrupt cognitive function
    James P Cleary
    Geriatric Research, Education and Clinical Center, Minneapolis Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA
    Nat Neurosci 8:79-84. 2005
    ....
  2. pmc The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
    Xun Xu
    BGI Shenzhen, Shenzhen, People s Republic of China
    Nat Biotechnol 29:735-41. 2011
    ..We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production...
  3. ncbi ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures
    Gina M Story
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 112:819-29. 2003
    ..Consistent with the expression of ANKTM1, we identify noxious cold-sensitive sensory neurons that also respond to capsaicin but not to menthol...
  4. pmc Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner
    Colin K Choi
    Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia 22908, USA
    Nat Cell Biol 10:1039-50. 2008
    ..From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization...
  5. ncbi Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin
    Michael Bandell
    Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA
    Neuron 41:849-57. 2004
    ..Collectively, these data demonstrate that TRPA1 activation elicits a painful sensation and provide a potential molecular model for why noxious cold can paradoxically be perceived as burning pain...
  6. ncbi Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family
    R I Montgomery
    Northwestern University Medical School, Department of Microbiology Immunology, Chicago, Illinois 60611, USA
    Cell 87:427-36. 1996
    ..The contribution of HVEM to HSV entry into human cells was demonstrable in activated T cells. HVEM, the first identified mediator of HSV entry, is a new member of the TNF/NGF receptor family...
  7. ncbi Spatial control of EGF receptor activation by reversible dimerization on living cells
    Inhee Chung
    Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nature 464:783-7. 2010
    ..Interestingly, dimers were enriched in the cell periphery in an actin- and receptor-expression-dependent fashion, resulting in a peripheral enhancement of EGF-induced signalling that may enable polarized responses to growth factors...
  8. ncbi PD-L2 is a second ligand for PD-1 and inhibits T cell activation
    Y Latchman
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 2:261-8. 2001
    ..Taken together, these studies show overlapping functions of PD-L1 and PD-L2 and indicate a key role for the PD-L-PD-1 pathway in regulatingT cell responses...
  9. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    ..Incubation of HeLa and CHO cells with the weak base ammonium chloride or the ionophore monensin, which elevate the low pH of organelles, blocked ..
  10. pmc Mapping the number of molecules and brightness in the laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA
    Biophys J 94:2320-32. 2008
    ....
  11. ncbi CHO cells in biotechnology for production of recombinant proteins: current state and further potential
    Jee Yon Kim
    Department of Biological Sciences, Graduate School of Nanoscience and Technology WCU, KAIST, 373 1 Kusong Dong, Yuseong Gu, Daejeon 305 701, Korea
    Appl Microbiol Biotechnol 93:917-30. 2012
    ....
  12. pmc Recreation of the terminal events in physiological integrin activation
    Feng Ye
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 188:157-73. 2010
    ....
  13. pmc The structure of an integrin/talin complex reveals the basis of inside-out signal transduction
    Nicholas J Anthis
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 28:3623-32. 2009
    ..These results show key structural features that explain the ability of talin to mediate inside-out TM signalling...
  14. pmc Pathways of DNA double-strand break repair during the mammalian cell cycle
    Kai Rothkamm
    Fachrichtung Biophysik, Universitat des Saarlandes, D 66421 Homburg Saar, Germany
    Mol Cell Biol 23:5706-15. 2003
    ..These data provide the first evaluation of the cell cycle-specific contributions of NHEJ and HR to the repair of radiation-induced versus replication-associated DSBs...
  15. pmc The selectivity of beta-adrenoceptor agonists at human beta1-, beta2- and beta3-adrenoceptors
    Jillian G Baker
    Institute of Cell Signalling, C Floor Medical School, Queen s Medical Centre, University of Nottingham, Nottingham, UK
    Br J Pharmacol 160:1048-61. 2010
    ..This study examined the affinity and intrinsic efficacy of 31 beta-adrenoceptor agonists at the three human beta-adrenoceptors to determine whether the current agonists are subtype selective because of affinity or intrinsic efficacy...
  16. pmc Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells
    Miguel Vicente-Manzanares
    Department of Cell Biology, University of Virginia, Charlottesville, VA 22908
    J Cell Biol 176:573-80. 2007
    ..It mediates each of the major component processes that drive migration, e.g., polarization, protrusion, adhesion assembly and turnover, polarity, signaling, and tail retraction, and it integrates spatially separated processes...
  17. ncbi Reconstructing and deconstructing agonist-induced activation of integrin alphaIIbbeta3
    Jaewon Han
    Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA
    Curr Biol 16:1796-806. 2006
    ..Many different signaling molecules that regulate integrin affinity have been described, but a pathway that connects agonist stimulation to talin binding and activation has not been mapped...
  18. ncbi Natural oligomers of the Alzheimer amyloid-beta protein induce reversible synapse loss by modulating an NMDA-type glutamate receptor-dependent signaling pathway
    Ganesh M Shankar
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 27:2866-75. 2007
    ..Our approach provides a quantitative cellular model for elucidating the molecular basis of Abeta-induced neuronal dysfunction...
  19. pmc Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules
    Jonathan A Hern
    Division of Physical Biochemistry, Medical Research Council National Institute for Medical Research, London NW7 1AA, United Kingdom
    Proc Natl Acad Sci U S A 107:2693-8. 2010
    ..In isolated CHO cells, receptors are randomly distributed over the plasma membrane...
  20. pmc Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks
    Naoya Uematsu
    Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    J Cell Biol 177:219-29. 2007
    ..We suggest a model in which DNA-PK(CS) phosphorylation/autophosphorylation facilitates NHEJ by destabilizing the interaction of DNA-PK(CS) with the DNA ends...
  21. pmc Measuring fast dynamics in solutions and cells with a laser scanning microscope
    Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of Illinois at Urbana Champaign, Illinois, USA
    Biophys J 89:1317-27. 2005
    ..This new type of data analysis has a broad application in biology and it provides a powerful tool for measuring fast as well as slower dynamic processes in cellular systems using any standard laser confocal microscope...
  22. ncbi Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic
    Pingsheng Liu
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9039, USA
    J Biol Chem 279:3787-92. 2004
    ..Based on the protein profile, the lipid droplet appears to be a complex, metabolically active organelle that is directly involved in membrane traffic and possibly phospholipid recycling. We propose the name adiposome for this organelle...
  23. pmc Transient receptor potential A1 is a sensory receptor for multiple products of oxidative stress
    David A Andersson
    Wolfson Centre for Age Related Diseases, King s College London, London SE1 1UL, United Kingdom
    J Neurosci 28:2485-94. 2008
    ..These data demonstrate that multiple agents produced during episodes of oxidative stress can activate TRPA1 expressed in sensory neurons...
  24. ncbi Dynamic activity of lipid droplets: protein phosphorylation and GTP-mediated protein translocation
    Rene Bartz
    Department of Cell Biology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9039, USA
    J Proteome Res 6:3256-65. 2007
    ....
  25. ncbi Identification of HE1 as the second gene of Niemann-Pick C disease
    S Naureckiene
    Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA
    Science 290:2298-301. 2000
    ..3, were found in NP-C2 patients but not in controls. Treatment of NP-C2 fibroblasts with exogenous recombinant HE1 protein ameliorated lysosomal accumulation of low density lipoprotein-derived cholesterol...
  26. ncbi Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor
    R J Geraghty
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, IL 60611, USA
    Science 280:1618-20. 1998
    ..HveC was expressed in human cells of epithelial and neuronal origin; it is the prime candidate for the coreceptor that allows both HSV-1 and HSV-2 to infect epithelial cells on mucosal surfaces and spread to cells of the nervous system...
  27. pmc Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor
    Heather M Scobie
    McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:5170-4. 2003
    ..These studies distinguish CMG2 as a second anthrax toxin receptor and identify a potent antitoxin that may prove useful for the treatment of anthrax...
  28. ncbi Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing
    Jennifer Becker
    Centrum für Biotechnologie, Universitat Bielefeld, 33594 Bielefeld, Germany
    J Biotechnol 156:227-35. 2011
    ..All relevant genes representing major steps in the N-glycosylation pathway of CHO cells were detected...
  29. pmc XRCC1 coordinates the initial and late stages of DNA abasic site repair through protein-protein interactions
    A E Vidal
    Departement de Radiobiologie et Radiopathologie, Commissariat a l Energie Atomique, UMR217 CNRS CEA, BP6, F 92265 Fontenay aux Roses, France
    EMBO J 20:6530-9. 2001
    ..The interaction described extends the coordinating role of XRCC1 to the initial step of the repair of DNA abasic sites...
  30. ncbi Evidence for the existence of an HP1-mediated subcode within the histone code
    Gwen Lomberk
    Gastroenterology Research Unit, Department of Medicine, and Mayo Clinic Cancer Center, Rochester, MN 55605, USA
    Nat Cell Biol 8:407-15. 2006
    ....
  31. ncbi XRCC1 and DNA strand break repair
    Keith W Caldecott
    Genome Damage and Stability Centre, University of Sussex, Science Park Road, BN1 9RQ, Falmer Brighton, UK
    DNA Repair (Amst) 2:955-69. 2003
    ..Models for the repair of single-strand breaks during base excision repair and at direct breaks are presented...
  32. ncbi A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells
    Tara L Deans
    Department of Biomedical Engineering, Center for BioDynamics and Center for Advanced Biotechnology, Boston University, Boston, MA 02215, USA
    Cell 130:363-72. 2007
    ....
  33. pmc Glycomics profiling of Chinese hamster ovary cell glycosylation mutants reveals N-glycans of a novel size and complexity
    Simon J North
    Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 285:5759-75. 2010
    ..Parent CHO cells grown in monolayer versus suspension culture had similar profiles of N- and O-GalNAc glycans, although the ..
  34. pmc A multifunctional pipette
    Alar Ainla
    Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivagen 10, SE 41296, Goteborg, Sweden
    Lab Chip 12:1255-61. 2012
    ..Device performance was characterised using both epifluorescence and total internal reflection fluorescence (TIRF) microscopy, resulting in ~200 ms and ~130 ms exchange times at ~100 nm and ~30 μm distances to the surface respectively...
  35. ncbi Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7
    N Barron
    National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland
    J Biotechnol 151:204-11. 2011
    ..Finally, we report the cloning and sequencing of the hamster-specific cgr-miR-7...
  36. pmc A C-terminal translocation signal required for Dot/Icm-dependent delivery of the Legionella RalF protein to host cells
    Hiroki Nagai
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, Room 354b, 295 Congress Avenue, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 102:826-31. 2005
    ..Thus, Legionella has the ability to engage synthesized substrate proteins and transfer them into host cells on contact, enabling Legionella to rapidly alter transport of the vacuole in which it resides...
  37. pmc {alpha}V{beta}3-integrin routes herpes simplex virus to an entry pathway dependent on cholesterol-rich lipid rafts and dynamin2
    Tatiana Gianni
    Department of Experimental Pathology, Section on Microbiology and Virology, Alma Mater Studiorum, University of Bologna, 40126 Bologna, Italy
    Proc Natl Acad Sci U S A 107:22260-5. 2010
    ..Here, we asked what cellular factors determine the pathway of HSV entry. CHO cells lack β(3)-integrin and the respective α-subunits' heterodimers...
  38. pmc Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression
    Stephan Fath
    Geneart Life Technologies, corrected BioPark, Regensburg, Germany
    PLoS ONE 6:e17596. 2011
    ..This is the first large-scale study addressing the influence of multiparameter optimization on autologous human protein expression...
  39. ncbi Uncoupling of cell growth and proliferation results in enhancement of productivity in p21CIP1-arrested CHO cells
    Jing Xiu Bi
    Department of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, United Kingdom
    Biotechnol Bioeng 85:741-9. 2004
    ....
  40. pmc Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8
    Youngah Jo
    Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390 9046, USA
    Proc Natl Acad Sci U S A 108:20503-8. 2011
    ..Variations in the concentrations of any one of these proteins may account for differences in cell- and/or tissue-specific regulation of reductase degradation...
  41. pmc Influenza virus entry and infection require host cell N-linked glycoprotein
    Victor C Chu
    Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 101:18153-8. 2004
    ..Finally, cleavage of N-glycans from wild-type CHO cells markedly reduced infection by influenza virus...
  42. ncbi Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1
    Jun Arii
    Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Nature 467:859-62. 2010
    ..The identification of NMHC-IIA as an HSV-1 entry receptor and the involvement of NM-IIA regulation in HSV-1 infection provide an insight into HSV-1 entry and identify new targets for antiviral drug development...
  43. ncbi Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases
    Pilar Redondo
    Macromolecular Crystallography Group, Spanish National Cancer Research Centre CNIO, c Melchor Fdez Almagro 3, 28029 Madrid, Spain
    Nature 456:107-11. 2008
    ....
  44. pmc Mutations in the N termini of herpes simplex virus type 1 and 2 gDs alter functional interactions with the entry/fusion receptors HVEM, nectin-2, and 3-O-sulfated heparan sulfate but not with nectin-1
    Miri Yoon
    Department of Microbiology Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Virol 77:9221-31. 2003
    ..The sequence of the N terminus determines whether nectin-2 or 3-O-sulfated heparan sulfate, as well as HVEM, can serve as entry/fusion receptors...
  45. pmc Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks
    Wael Y Mansour
    Laboratory of Radiobiology and Experimental Radiation Oncology, Department of Radiotherapy and Radiation Oncology, University Medical School Hamburg Eppendorf, Martinistrasse 52, D 20246 Hamburg, Germany
    Nucleic Acids Res 36:4088-98. 2008
    ..Furthermore, the cellular choice of repair pathways is reversible and can be influenced at the level of effector proteins such as Ku80 or Rad51...
  46. ncbi Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses
    S Liu
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 402:676-81. 1999
    ..Thus, the tight association of paxillin with the alpha4 tail leads to distinct biochemical and biological responses to integrin-mediated cell adhesion...
  47. ncbi ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I
    Ingrid C Gelissen
    Centre for Vascular Research, School of Medical Sciences, University of New South Wales, Kensington, Australia
    Arterioscler Thromb Vasc Biol 26:534-40. 2006
    ..To study the acceptor specificity for human ABCG1 (hABCG1)-mediated cholesterol efflux...
  48. ncbi Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5
    J M Bergelson
    Division of Infectious Diseases, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 275:1320-3. 1997
    ..Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors...
  49. ncbi Nogo-A inhibits neurite outgrowth and cell spreading with three discrete regions
    Thomas Oertle
    Brain Research Institute, University of Zurich, and Department of Biology, Swiss Federal Institute of Technology, CH 8057 Zurich, Switzerland
    J Neurosci 23:5393-406. 2003
    ..This suggests that Nogo-A could have a second membrane topology. The two proposed topological variants may have different intracellular as well as extracellular functions...
  50. pmc Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene
    R S Tebbs
    Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94551 0808, USA
    Proc Natl Acad Sci U S A 92:6354-8. 1995
    ..0-kb mRNA on Northern blots. The cDNA was mapped by fluorescence in situ hybridization to human chromosome 14q32.3, which was consistent with the chromosome concordance data of two independent hybrid clone panels...
  51. ncbi The autophagic pathway is actively modulated by phase II Coxiella burnetii to efficiently replicate in the host cell
    Patricia S Romano
    Laboratorio de Biologia Celular y Molecular, IHEM CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo Mendoza, 5500, Argentina
    Cell Microbiol 9:891-909. 2007
    ..This intersection with the autophagic pathway delays fusion with the lysosomal compartment possibly favouring the intracellular differentiation and survival of the bacteria...
  52. ncbi Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells
    Imke A J Dreesen
    Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH 4058 Basel, Switzerland
    Biotechnol Bioeng 108:853-66. 2011
    ..mTOR-based engineering of mammalian production cell lines may therefore have a promising future in biopharmaceutical manufacturing of human therapeutic proteins...
  53. ncbi Dynamic mRNA and miRNA profiling of CHO-K1 suspension cell cultures
    Juan A Hernández Bort
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Biotechnol J 7:500-15. 2012
    ..In addition, as miRNAs generally act as negative post-transcriptional regulators of mRNAs, we looked for changes in their expression that were inverse to those of their predicted target mRNAs...
  54. pmc Overexpression of Eag1 potassium channels in clinical tumours
    Bernhard Hemmerlein
    Department of Pathology, Georg August University, Gottingen, Germany
    Mol Cancer 5:41. 2006
    ..We have now studied the expression pattern of the Eag1 channel in a large range of normal and tumour tissues from different collections utilising molecular biological and immunohistochemical techniques...
  55. pmc Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells
    Jung Hyun Kim
    Laboratories of Molecular Pharmacology, National Cancer Institute, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:20048-53. 2011
    ..This generation of human artificial chromosomes should be suitable for studies of gene function and therapeutic applications...
  56. pmc Identification of the gene encoding alkylglycerol monooxygenase defines a third class of tetrahydrobiopterin-dependent enzymes
    Katrin Watschinger
    Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, A 6020 Innsbruck, Austria
    Proc Natl Acad Sci U S A 107:13672-7. 2010
    ..A functional library screen using pools of plasmids of a rat liver expression library transfected to CHO cells was also unsuccessful...
  57. ncbi Identification of the cellular receptor for anthrax toxin
    K A Bradley
    McArdle Laboratory for Cancer Research, University of Wisconsin Madison, 1400 University Avenue, Madison, Wisconsin 53706, USA
    Nature 414:225-9. 2001
    ..In addition, a soluble version of this domain can protect cells from the action of the toxin...
  58. pmc Amyloid beta 42 peptide (Abeta42)-lowering compounds directly bind to Abeta and interfere with amyloid precursor protein (APP) transmembrane dimerization
    Luise Richter
    Institut fur Chemie und Biochemie, Freie Universitat Berlin, 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 107:14597-602. 2010
    ..We conclude that these GSMs decrease Abeta42 levels by modulating APP-TMS interactions. This effect specifically emphasizes the importance of the dimeric APP-TMS as a promising drug target in Alzheimer's disease...
  59. ncbi Expression cloning of a functional glycoprotein ligand for P-selectin
    D Sako
    Genetics Institute, Small Molecule Drug Discovery Group, Cambridge, Massachusetts 02140
    Cell 75:1179-86. 1993
    ..A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin...
  60. pmc A carboxy-terminal inter-helix linker as the site of phosphatidylinositol 4,5-bisphosphate action on Kv7 (M-type) K+ channels
    Ciria C Hernandez
    Department of Physiology, MS 7756, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA
    J Gen Physiol 132:361-81. 2008
    ..The results are likely to shed light on the general structural mechanisms of phosphoinositide regulation of ion channels...
  61. pmc Cellular and viral requirements for rapid endocytic entry of herpes simplex virus
    Anthony V Nicola
    Medical Virology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1888, USA
    J Virol 78:7508-17. 2004
    ..Thus, similar to entry via direct penetration at the cell surface, HSV entry into cells by wortmannin-sensitive endocytosis is efficient, involves rapid cellular uptake of viral particles, and requires gB, gD, and gH-gL...
  62. pmc Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells
    Benedict Hebert
    Department of Physics and Department of Chemistry, McGill University, Montreal, Quebec, Canada
    Biophys J 88:3601-14. 2005
    ..studies of alpha-actinin/enhanced green fluorescent protein fusion constructs at the basal membrane of living CHO cells. Using space-time image correlation spectroscopy (STICS), we are able to measure protein fluxes with magnitudes ..
  63. ncbi A subset of NSAIDs lower amyloidogenic Abeta42 independently of cyclooxygenase activity
    S Weggen
    Department of Neurosciences, University of California San Diego, La Jolla, California 92093, USA
    Nature 414:212-6. 2001
    ....
  64. ncbi Regulation of recombinant monoclonal antibody production in chinese hamster ovary cells: a comparative study of gene copy number, mRNA level, and protein expression
    Zhou Jiang
    The Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Biotechnol Prog 22:313-8. 2006
    We performed a comparative analysis on CHO cells with varying recombinant monoclonal antibody production rates (qAb) and investigated the regulation of MAb production...
  65. ncbi Impaired thermosensation in mice lacking TRPV3, a heat and camphor sensor in the skin
    Aziz Moqrich
    Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA
    Science 307:1468-72. 2005
    ..Camphor activated cultured primary keratinocytes but not sensory neurons, and this activity was abolished in TRPV3 null mice. Therefore, heat-activated receptors in keratinocytes are important for mammalian thermosensation...
  66. ncbi Stomatin modulates gating of acid-sensing ion channels
    Margaret P Price
    Department of Internal Medicine, University of Iowa College of Medicine, 200 Hawkins Dr, Iowa City, IA 52242, USA
    J Biol Chem 279:53886-91. 2004
    ..Our findings indicate that modulation of DEG/ENaC channels by stomatin-like proteins is evolutionarily conserved and may have important implications for mammalian nociception and mechanosensation...
  67. pmc The structure and function of a foot-and-mouth disease virus-oligosaccharide receptor complex
    E E Fry
    The Laboratory of Molecular Biophysics, Rex Richards Building, South Parks Road, Oxford OX1 3QU, UK
    EMBO J 18:543-54. 1999
    ..We postulate that this site is a conserved feature of FMDVs, such that in the infected animal there is a biological advantage to low affinity, or more selective, interactions with glycosaminoglycan receptors...
  68. pmc Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair
    Qi Ding
    College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
    Mol Cell Biol 23:5836-48. 2003
    ..However, autophosphorylation at these sites may not be sufficient for kinase disassembly...
  69. ncbi RBE for carbon track-segment irradiation in cell lines of differing repair capacity
    W K Weyrather
    Gesellschaft fur Schwerionenforschung, Darmstadt, Germany
    Int J Radiat Biol 75:1357-64. 1999
    ..RBE values for cell inactivation at different survival levels were determined and the differences in the RBE-LET patterns were compared with the individual sensitivity to photon irradiation of the different cell lines...
  70. ncbi Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides
    Bing Hao Luo
    CBR Institute fo Biomedical Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:10215-21. 2004
    ..e. that axial movement in the C-terminal direction of the alpha7-helix is linked to rearrangement of the I-like domain metal ion-dependent adhesion site into a high affinity conformation...
  71. ncbi Sphingosine 1-phosphate (S1P) receptor subtypes S1P1 and S1P3, respectively, regulate lymphocyte recirculation and heart rate
    M Germana Sanna
    Department of Immunology, The Center for Mass Spectrometry, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:13839-48. 2004
    ....
  72. ncbi Macrolide-based transgene control in mammalian cells and mice
    Wilfried Weber
    Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Zurich, CH 8093 Zurich, Switzerland
    Nat Biotechnol 20:901-7. 2002
    ....
  73. ncbi The use of Chinese hamster ovary (CHO) cells in the study of ion channels
    Nikita Gamper
    Department of Physiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio TX 78229, USA
    J Pharmacol Toxicol Methods 51:177-85. 2005
    The line of epithelial-like Chinese hamster ovary (CHO) cells was initiated by T.T. Puck in 1957. Since then, CHO cells have become a widely used mammalian expression system in industry and science...
  74. ncbi Mammalian mutation pressure, synonymous codon choice, and mRNA degradation
    Jubao Duan
    Department of Psychiatry, The University of Chicago, 924 East 57th Street, R 004, Chicago, IL 60637, USA
    J Mol Evol 57:694-701. 2003
    ..This may explain why nucleotide motif preferences are very similar in transcribed and nontranscribed mammalian DNA even though the preferences appear to be adaptive only in transcribed DNA...
  75. ncbi The N terminus of the MUC2 mucin forms trimers that are held together within a trypsin-resistant core fragment
    Klaus Godl
    Department of Medical Biochemistry, Goteborg University, Gothenburg, Sweden
    J Biol Chem 277:47248-56. 2002
    ....
  76. pmc Complete genetic correction of ips cells from Duchenne muscular dystrophy
    Yasuhiro Kazuki
    Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University, Yonago, Japan
    Mol Ther 18:386-93. 2010
    ..Therefore, the combination of patient-specific iPS cells and HAC-containing defective genes represents a powerful tool for gene and cell therapies...
  77. pmc Amyloidogenic processing but not amyloid precursor protein (APP) intracellular C-terminal domain production requires a precisely oriented APP dimer assembled by transmembrane GXXXG motifs
    Pascal Kienlen-Campard
    Center for Neurosciences, Experimental Pharmacology Unit, Universite Catholique de Louvain, B 1200 Brussels, Belgium
    J Biol Chem 283:7733-44. 2008
    ..Increased dimerization of the TM APP C-terminal domain did not affect AICD production...
  78. pmc Segregation and activation of myosin IIB creates a rear in migrating cells
    Miguel Vicente-Manzanares
    Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 183:543-54. 2008
    ....
  79. ncbi Enhanced sialylation of EPO by overexpression of UDP-GlcNAc 2-epimerase/ManAc kinase containing a sialuria mutation in CHO cells
    Kaya Bork
    Institut fur Biochemie und Molekularbiologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Arnimallee 22, 14195 Berlin Dahlem, Germany
    FEBS Lett 581:4195-8. 2007
    ..Our data show that sialuria-mutated-GNE over-expressing cells are the perfect platform to express highly sialylated therapeutic proteins, such as EPO...
  80. ncbi Regulation of Wnt signaling and embryo patterning by an extracellular sulfatase
    G K Dhoot
    Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK
    Science 293:1663-6. 2001
    ....
  81. pmc Glycoprotein D receptor-dependent, low-pH-independent endocytic entry of herpes simplex virus type 1
    Richard S B Milne
    Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 215 Levy Building, 240 South 40th Street, Philadelphia, PA 19104 6002, USA
    J Virol 79:6655-63. 2005
    ..Resistance to similar reagents should therefore not be taken as proof of HSV entry by a nonendosomal pathway. These data define a novel gD receptor-dependent acid-independent endocytic entry pathway for HSV...
  82. ncbi Cell fusion induced by herpes simplex virus glycoproteins gB, gD, and gH-gL requires a gD receptor but not necessarily heparan sulfate
    P E Pertel
    Department of Medicine, Northwestern University Medical School, Chicago, Illinois, 60611, USA
    Virology 279:313-24. 2001
    ..Overall, the viral and cellular determinants of HSV-1-induced cell fusion are similar to those for viral entry, except that HSV-1 entry is significantly enhanced by binding of virus to cell surface heparan sulfate...
  83. ncbi Glycosylation of Eag1 (Kv10.1) potassium channels: intracellular trafficking and functional consequences
    Joanna Napp
    Max Planck Institute for Experimental Medicine, Gottingen, Germany
    J Biol Chem 280:29506-12. 2005
    ..Correct complex glycosylation is required for proper trafficking of Eag1 to the plasma membrane but is also crucial for the correct function of channels already inserted in the membrane...
  84. ncbi A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry
    D Shukla
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Cell 99:13-22. 1999
    ....
  85. ncbi A cell surface protein with herpesvirus entry activity (HveB) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus
    M S Warner
    Department of Microbiology Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    Virology 246:179-89. 1998
    ..Antibodies specific for HveB blocked infection of HveB-expressing CHO cells and a human fibroblastic cell strain HEL299...
  86. ncbi 25 years of recombinant proteins from reactor-grown cells - where do we go from here?
    David L Hacker
    Ecole Polytechnique Federale de Lausanne EPFL, Laboratory of Cellular Biotechnology, CH 1015 Lausanne, Switzerland
    Biotechnol Adv 27:1023-7. 2009
    ..Other emerging trends in protein manufacturing that are discussed include the use of disposal bioreactors and transient gene expression. We specifically highlight current research in our own laboratories...
  87. ncbi A study of monoclonal antibody-producing CHO cell lines: what makes a stable high producer?
    Janet Chusainow
    Bioprocessing Technology Institute, A STAR Biomedical Sciences Institutes, 20 Biopolis Way, 06 01 Centros, Singapore 138668, Singapore
    Biotechnol Bioeng 102:1182-96. 2009
    ....
  88. pmc Ether à go-go potassium channel expression in soft tissue sarcoma patients
    Fernanda Mello de Queiroz
    Divisão de Farmacologia, Coordenação de Pesquisa, Instituto Nacional do Cancer, Rio de Janeiro, Brazil
    Mol Cancer 5:42. 2006
    ..Our purpose was to determine the frequency of expression of Eag1 in soft tissue sarcoma and its potential clinical implications...
  89. pmc Latent transforming growth factor-beta binding protein domains involved in activation and transglutaminase-dependent cross-linking of latent transforming growth factor-beta
    I Nunes
    Department of Cell Biology, Kaplan Cancer Center, New York University Medical Center, New York 10016, USA
    J Cell Biol 136:1151-63. 1997
    ..of either the amino-terminal 293 (deltaN293) or 441 (deltaN441) amino acids were expressed transiently in CHO cells. Analysis of the LTBP-1S content in matrices of transfected CHO cultures revealed that deltaN293 LTBP-1S was ..
  90. pmc Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking
    Yasuhiro M Umemura
    Membrane Mechanisms Project, International Cooperative Research Project, Japan Science and Technology Agency, Institute for Integrated Cell Material Sciences, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Biophys J 95:435-50. 2008
    ..up to 65 Hz, showed that the transmembrane MHC class II protein and its GPI-anchored modified form expressed in CHO cells undergo simple Brownian diffusion, without any influence of actin depolymerization with cytochalasin D...
  91. ncbi The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry
    C C Bleul
    The Center for Blood Research, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 382:829-33. 1996
    ....
  92. pmc Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica
    Mohammad Abu Yousuf
    Department of Parasitology, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku, Tokyo, Japan
    Eukaryot Cell 9:926-33. 2010
    ..PNT that is compartmentalized to the acidic compartments is unprecedented in eukaryotes and may possess a unique physiological role in E. histolytica...
  93. ncbi Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation
    T Blunt
    Wellcome Cancer Research Campaign Institute, Cambridge University, England
    Cell 80:813-23. 1995
    ..As DNA-PK binds to DNA ends and is activated by these structures, our findings provide novel insights into V(D)J recombination and DNA repair processes...
  94. ncbi T-cell co-stimulation through B7RP-1 and ICOS
    S K Yoshinaga
    Amgen Inc, Thousand Oaks, California 91320, USA
    Nature 402:827-32. 1999
    ..Therefore, B7RP-1 exhibits co-stimulatory activities in vitro and in vivo. ICOS and B7RP-1 define a new and distinct receptor-ligand pair that is structurally related to CD28-B7 and is involved in the adaptive immune response...
  95. pmc Tissue culture adaptation of foot-and-mouth disease virus selects viruses that bind to heparin and are attenuated in cattle
    D Sa-Carvalho
    Plum Island Animal Disease Center, Agricultural Research Service, North Atlantic Area, U S Department of Agriculture, Greenport, New York 11944, USA
    J Virol 71:5115-23. 1997
    ..variants with distinct plaque morphologies on BHK cells: a small, clear-plaque virus that replicates in BHK and CHO cells, and a large, turbid-plaque virus that only grows in BHK cells...
  96. pmc Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide
    E Lien
    The Maxwell Finland Laboratory for Infectious Diseases, Boston Medical Center, Boston, Massachusetts 02118, USA
    J Clin Invest 105:497-504. 2000
    ..These data suggest that TLR4 is the central lipid A-recognition protein in the LPS receptor complex...
  97. ncbi SLAM (CDw150) is a cellular receptor for measles virus
    H Tatsuo
    Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
    Nature 406:893-7. 2000
    ..The identification of SLAM as a receptor for measles virus opens the way to a better understanding of the pathogenesis of measles virus infection, especially the immunosuppression induced by measles virus...
  98. pmc Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells
    Vaibhav Tiwari
    Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois, Chicago, IL, USA
    FEBS J 275:5272-85. 2008
    ..Our findings shed new light on the cellular and molecular mechanisms that help the virus to enter the cells of the inner eye...
  99. ncbi Mouse taste buds use serotonin as a neurotransmitter
    Yi Jen Huang
    Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33136, USA
    J Neurosci 25:843-7. 2005
    ..These experiments strongly implicate serotonin as a taste bud neurotransmitter and reveal unexpected transmitter release mechanisms...
  100. pmc Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics
    Anjana Nayal
    Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA
    J Cell Biol 173:587-9. 2006
    ..These adhesions appear to be regulated by the GIT1-PIX-PAK module near the leading edge...
  101. pmc Membrane requirement for folding of the herpes simplex virus 1 gB cytodomain suggests a unique mechanism of fusion regulation
    Jessica L Silverman
    Department of Molecular Biology and Microbiology and Graduate Program in Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, USA
    J Virol 86:8171-84. 2012
    ..Taken together, our data suggest that the cytodomain of gB regulates fusion by a novel mechanism in which membrane interaction plays a key role...

Research Grants64

  1. Novel DNA-Launched Attenuated Vaccine for VEE Virus
    PETER M PUSHKO; Fiscal Year: 2012
    ..The i-DNA clones will be transfected in CHO cells and live attenuated viruses will be harvested from culture media...
  2. Mechanism and functional role of AKAP79/150 in M current control and excitability
    Mark S Shapiro; Fiscal Year: 2010
    ..The elucidation of the mechanisms and functional role of AKAP79/150 in M-channel activity will shed light on how the nervous system functions in health and disease. ..
  3. Screening for allosteric modulators of the protease activated receptor 4
    Heidi E Hamm; Fiscal Year: 2013
    ..screen, 2) we will identify compounds which inhibit thrombin stimulation of PAR4 and are selective for PAR4 in CHO cells and human platelets which express PAR4 endogenously, 3) we will determine whether the mechanism of inhibition is ..
  4. Novel High Affinity VEGF Analogs For Targeted Imaging of Thyroid Cancer
    BRUCE DALE WEINTRAUB; Fiscal Year: 2013
    ..proliferation;produce and purify 5-10 mg amounts of the final 3 glycosylated and optimally sialylated analogs in CHO cells in roller bottles;(2) Develop novel pegylated polymeric "stealth" nanoparticles targeted by covalently coupled ..
  5. GENETIC CONTROL OF PROTEOGLYCAN METABOLISM
    Jeffrey D Esko; Fiscal Year: 2010
    ..In additionwe will explore how the individual isozymes affects heparan sulfate biosynthesis in transfected cells. ..
  6. Metabolic Engineering of Chinese Hamster Ovary Cells for Production of Heparin
    Susan T Sharfstein; Fiscal Year: 2011
    ..b>CHO cells are widely used in the biotechnology/biopharmaceutical industry for the production of recombinant therapeutic ..
  7. Bifunctional antibodies with targeted CNS delivery against West Nile virus
    Qiang Chen; Fiscal Year: 2013
    ..of hE16 (TfR-Bif) with the potential for enhanced crossing of the blood-brain barrier (BBB) can be expressed in CHO cells or plants...
  8. Drug Discovery for Multiple Hereditary Exostoses
    Jeffrey D Esko; Fiscal Year: 2013
    ..abstract_text> ..
  9. The Role of MTP in Lipid Droplet Formation in Adipocytes
    Larry L Swift; Fiscal Year: 2013
    ..In addition, when MTP is transfected into CHO cells and lipid droplet formation stimulated, larger droplets are formed compared to those formed in cells without MTP...
  10. GENETIC SUSCEPTIBILITY TO GRAVES'-LIKE HYPERTHYROIDISM IN MICE
    Sandra M McLachlan; Fiscal Year: 2013
    ..TSAb bioactivity will be compared using CHO cells expressing human and mouse TSHR. Identify by linkage analysis the chromosomes/loci associated with these traits...
  11. Potent Phage T4 Derived V2 Immunogens as HIV Vaccines
    Venigalla B Rao; Fiscal Year: 2013
    ..virus sequences, different glycosylation patterns, and random mutations will be constructed and expressed in CHO cells as secretory proteins...
  12. Regulation of Platelet Glycoprotein (GP) Ib-IX Complex Function by Membrane Lipid
    Yuandong Peng; Fiscal Year: 2013
    ..isomerase (PDI) associates with the GP Ib-IX complex in both platelets and the GP Ib-IX complex-expressing CHO cells, an interaction only being seen in GEMs domain...
  13. Regulation of the early events of platelet activation
    Lawrence F Brass; Fiscal Year: 2013
    ..These events can be recapitulated in transfected CHO cells. Based on these observations, we propose that spinophilin first sequesters RGS proteins, allowing signaling to ..
  14. Elucidating the role of the NPC1L1 cholesterol uptake receptor in HCV infection
    Susan L Uprichard; Fiscal Year: 2013
    ..Based on the observation that exogenous expression of NPC1L1 on the surface of CHO cells results in binding of the cholesterol-enriched HCV virion and the fact that NPC1L1 exhibits restricted tissue ..
  15. Neurotrophin Drug Development for Parkinson's Disease
    William M Pardridge; Fiscal Year: 2012
    ..The present research will test in experimental PD in mice the efficacy of a new form of GDNF, wherein the neurotrophin is re-engineered as a fusion protein with a monoclonal antibody that crosses the BBB via receptor-mediated transport. ..
  16. A novel approach for developing GPCR subtype specific antibodies
    Xiaomin Fan; Fiscal Year: 2012
    ..clones with a series of assays on CHO cell-lines expressing each GPCR compared to binding on wild-type CHO cells to determine their specificity and map their respective epitopes on their cognate GPCRs...
  17. Development of a multimeric CD40 ligand vaccine adjuvant
    RICHARD SYD KORNBLUTH; Fiscal Year: 2013
    ..SBIR Phase 1 project, MegaCD40L will be advanced toward the clinic by developing research cell banks of cGMP CHO cells that produce human and murine MegaCD40L...
  18. Targeting Agents for Human and Canine Lymphoma
    Kit Lam; Fiscal Year: 2009
    ..Program 2 involves the use of a series of CHO cells, that have been stably transfected with wild type or mutant alpha4 and/or beta1 integrin genes, to evaluate the ..
  19. Compound library screening using Giardia labmblia high throughput viability assay
    Osnat Herzberg; Fiscal Year: 2012
    ..We propose to screen the NIH compound library and investigate the selectivity of hit compounds using initially CHO cells and later CACO-2 cells...
  20. Selective Opioid Antagonists as Medications for Drug Abuse
    Frank Ivy Carroll; Fiscal Year: 2013
    ..binding produced by selective [unreadable], d, and ? agonists using cloned human opioid receptors expressed in CHO cells. Analogs meeting set criteria for ? potency and selectivity will be evaluated in an in vivo PK study to ..
  21. Thiol Specific Fluorogenic Agents for Laser Scanning Confocal Microscopy
    Xiangming Guan; Fiscal Year: 2010
    ..This proposed application is aimed to develop a method which can be used to detect the status of oxidative stress in intact cells. ..
  22. Fluorescence Correlation Quantitation of Insulin-like Growth Factor and Insulin R
    B George Barisas; Fiscal Year: 2013
    ..Initial experiments will involve a model system consisting of CHO cells simultaneously expressing green and red fluorescent forms of IR and IGF1R (IR-GFP and IGF1R-mCherry), each ..
  23. Association of tissue factor pathway inhibitor with endothelium
    Alan E Mast; Fiscal Year: 2012
    ..SA1 will compare the anti-TF activity of different forms of GPI-anchored TFPI on the surface of CHO cells and primary mouse endothelial cells. SA2 will examine how the mode of membrane association (GPI-anchored vs...
  24. Genetic Engineering of Cell Lines for Bioproduction
    Derek Jantz; Fiscal Year: 2010
    ..We propose to use genetic engineering technology to produce mammalian cell lines that are optimized to be "factories" for the inexpensive production of higher quality protein pharmaceuticals. ..
  25. Rapid parallel 3D confocal/fluorescence cell imaging with varifocal lens
    Guoqiang Li; Fiscal Year: 2010
    ..Specifically, we will study live cells (CHO cells expressing specific surface receptors;e.g...
  26. Cannabinoid Modulation of Capsaicin-Sensitive Nociceptors
    Kenneth M Hargreaves; Fiscal Year: 2010
    ..we have discovered that arachidonyl-2-chloroethylamide (ACEA) selectively activates TRPV1 in transfected CHO cells, whereas WIN selectively activates TRPA1, and that AM1241 preferentially activates a heretofore unreported TRPV1/..
  27. Mixed Kappa-Mu Opioids: Synthesis and Evaluation
    John L Neumeyer; Fiscal Year: 2012
    ..Neumeyer, Ph.D. and Elena Chartoff, Ph.D., and the pharmacological evaluation component will be conducted at the University of Rochester under the direction of Jean M. Bidlack, Ph.D. ..
  28. Development of Orexin-1 Receptor Antagonists to Prevent Drug Relapse
    Paul J Kenny; Fiscal Year: 2011
    ..of intracellular calcium signaling via membrane potential dyes and a fluorescent plate reader (FLIPR) in CHO cells stably expressing human OX1 receptors (OX1 receptors couple to Gq)...
  29. Design and Development of Model-based Selection Systems in Mammalian Cell Lines
    RENATA USAITE BLACK; Fiscal Year: 2011
    ..In Aim III, we will evaluate the development and implementation of a model-based selection system in CHO cells by comparing experimentally generated cell culture data with those calculated by the reconstructed model...
  30. Development of M5 Selective Muscarinic Antagonists
    Guangrong Zheng; Fiscal Year: 2012
    ..Druggable virtual hits will be evaluated in receptor binding assays using CHO cells expressing hM1-hM5...
  31. Mechanisms of chemoattractant receptor regulation
    HEINI MIETTINEN; Fiscal Year: 2005
    ..This will be carried out in CHO cells expressing wild-type FPR and various mutant FPRs...
  32. A new method to measure taste bud function
    Stephen D Roper; Fiscal Year: 2013
    ..Our biosensors consist of genetically-engineered CHO cells that are very sensitive to specific transmitters...
  33. PENETRATION OF MACROMOLEULCES INTO CELLS
    H Ryser; Fiscal Year: 1999
    ..Disulfide cleavage in CHO cells pulse labeled with 125I-tyn-SS-PDL begins without lag and continues for several hours...
  34. BIOLOGY OF ATRIAL NATRIURETIC FACTOR AND ITS RECEPTORS
    Thomas Maack; Fiscal Year: 2000
    ..GCA receptor) will be performed with recombinant wild-type and mutated receptors transfected into 293 and/or CHO cells. To test whether the very rapid dissociation of receptor- ligand complexes is modulated by the interaction of ..
  35. CELLULAR AND MOLECULAR STUDIES OF VESTIBULAR HAIR CELLS
    MANNING CORREIA; Fiscal Year: 2007
    ..chimera insertion will be assayed by making single channel recordings from the mutated molecules expressed in CHO cells. It will be determined if changes in amino acids in the ECL change the unusually high open probability, P(O) ..
  36. OPHTHALMOPATHY OF GRAVES DISEASE
    Juan Jaume; Fiscal Year: 2001
    ..1)Generation of recombinant human TSHR -antigens:- (i) Wild-type TSHR and TSHR ectodomain: Stably transfected CHO cells expressing full- length wild-type and truncated TSHR ectodomain are available, but due to the low levels of ..
  37. ENGINEERING INTEGRIN--MEDIATED CELL MIGRATION
    Douglas Lauffenburger; Fiscal Year: 2002
    ..adhesiveness will be studied by measuring the intercellular levels of activated FAK and MAPK in wild type CHO cells as a function of integrin number (by altering integrin expression levels), ECM ligand concentration and integrin/..
  38. Recombinant human serum albumin for use in cell culture media
    Ning Huang; Fiscal Year: 2007
    ..In addition, monoclonal antibody production by AE1 and CHO cells will be quantitatively measured using IgG-specific enzyme linked immunosorbent assays...
  39. RADIATION INDUCED CHROMATIN CHANGES IN A DEFINED ORIGIN
    James Larner; Fiscal Year: 2001
    ..radiation specifically down-regulates initiation in the early firing dihydrofolate reductase (DHFR) origin in CHO cells and demonstrated that this S-phase damage-sensing (SDS) pathway also inhibits initiation in mid-firing rDNA ..
  40. TAS::75 0893::TAS RAPID LARGE SCALE PRODUCTION OF PROTEIN THERAPEUTICS
    Wenda Gao; Fiscal Year: 2011
    ..The Contractor will provide contract CMO services for generating stable, high-producing CHO cells lines for large-scale production of recombinant antibodies and vaccines.
  41. GENETIC ANALYSIS OF ENDOCYTOSIS
    Monty Krieger; Fiscal Year: 1990
    ..Repeat-free genomic probes will then be used for cDNA cloning and the cDNA will be sequenced. Antibodies to this gene's product will be prepared for ultrastructural and biochemical analyses designed to elucidate the function of ldlC...
  42. MODULATION OF ORI-BETA REPLICATION ORIGIN BY FISH OIL
    Nawfal Istfan; Fiscal Year: 2004
    ..in the activity at the replication origin (ori-beta) located within the dihydrofolate reductase (DHFR) gene in CHO cells. We have also shown that fish oil alters the chromatin structure in cells where the effect of this oil on S-..
  43. Modulation of Adenoviral Infections by CD44
    CRISTHIAN J ILDEFONSO; Fiscal Year: 2010
    ..Binding will be determined in the presence and absence of HA using CHO cells that do not express the CAR or CD46 adenoviral receptors but do express CD44...
  44. BCR KINASE IN PDGF-MED EFFECTS IN VASCULAR SMOOTH MUSCLE
    Jun Ichi Abe; Fiscal Year: 2002
    ..that Bcr kinase is rapidly activated by PDGF in VSMC and Bcr overexpression enhances ERK1/2 activity in CHO cells expressing PDGF receptor...
  45. FUNCTION DOMAINS IN THE NUCLEAR LAMIN PROTEINS
    Frank McKeon; Fiscal Year: 1992
    ..both random and site-specific mutations in the coding regions of the human lamin cDNAs and expressing them in CHO cells. The human lamins will be detected and distinguished from the endogenous CHO lamins by using a human-specific ..
  46. EPH RECEPTORS AND EPHRINS IN VISUAL SYSTEM ORGANIZATION
    Elena Pasquale; Fiscal Year: 2002
    ..The investigator has generated CHO cells transfected with EphB2 and will generate ephrin-B1 expressing cells...
  47. Glycosylation Mutants of Mice and Animal Cells
    PAMELA M STANLEY; Fiscal Year: 2010
    ..Glycosyltransferase gene mutants of mice, embryonic stem (ES) cells and CHO cells will be used to investigate the basis of cell-type specific blocks to differentiation and transformation caused ..
  48. REGULATED ENDOCYTIC TRAFFIC IN FIBROBLASTS
    Timothy McGraw; Fiscal Year: 2004
    ..demonstrated that vpTR is trafficked by a specialized, insulin-regulated, dynamic retention mechanism in CHO cells. The specific aims of this proposal are to characterize the motifs that determine dynamic retention within the ..
  49. GLYCOSYLATION MUTANTS OF ANIMAL CELLS
    Pamela Stanley; Fiscal Year: 1999
    ..3)Fuc-T)] by transfection of human genomic DNA into CHO cells and gene rescue in lambda vectors...
  50. Targeting Liposomal Daunorubicin to Myeloid Leukemia
    Xing Pan; Fiscal Year: 2004
    ..cells cannot bind folate in contrast to that in primary AML cells, KG- 1 cells, and FR-beta-transfected CHO cells, all of which mediate selective uptake and cytotoxicity of folate-coated liposomal doxorubicin (f-L-DOX)...
  51. Regulation of mitochondrial glycerol-3-P acyltranferase
    Rosalind Coleman; Fiscal Year: 2004
    ..The proposed experiments will analyze the activity of different truncated and mutated mitoGPATs expressed in CHO cells. We will determine which fragments of the mitoGPAT are essential for its proper function...
  52. Novel Strategy for Animal Model Testing of HCMV Vaccines
    Mark Schleiss; Fiscal Year: 2007
    ..These studies will represent the first efficacy test of a human CMV subunit vaccine in a small animal model of congenital CMV infection. [unreadable] [unreadable]..
  53. MECHANISMS UNDERLYING MELATONIN RECEPTOR FUNCTION
    PAULA WITT ENDERBY; Fiscal Year: 1999
    ..In addition, melatonin exposure dramatically alters tubulin assembly in CHO cells transfected with the mt1 melatonin receptor...
  54. MEMBRANE PROTEOGLYCANS OF CHINESE HAMSTER OVARY CELLS
    KAREN BAME; Fiscal Year: 1991
    ..proteoglycan synthesis mutant, pgsE-606, suggests that there are populations of heparan sulfate proteoglycans in CHO cells. We propose to characterize the populations of cell surface proteoglycans on wild-type and pgsE-606 CHO cells...
  55. MYELIN PO PROTEIN--EXPRESSION/SORTING AND ADHESION
    Marie Filbin; Fiscal Year: 1992
    ..and without the reporter peptide is expressed/sorted in an identifical manner, both will be characterized in CHO cells, prior to the studies with Schwann cells...
  56. REGULATORY CELL MUTANTS IN CHOLESTEROL BIOSYNTHESIS
    Ta Yuan Chang; Fiscal Year: 1991
    ..In addition, the partial or full length cDNA for M1 protein will be engineered into prokaryotic expression vectors to produce fusion proteins, against which antibodies can be raised...
  57. HOW DOES P24/CD9 FUNCTION IN CELL ADHESION
    Lisa Jennings; Fiscal Year: 1999
    ..that: 1) fibronectin is a ligand for affinity-purified native CD9 as well as recombinant CD9; 2) CD9 transfected CHO cells spread to a greater extent on fibronectin coated surfaces; 3) CD9 transfected CHO cells have greater mobility on ..
  58. IMMUNOLOGICAL ANALYSIS OF LDL RECEPTOR DEFECTIVE CELLS
    Monty Krieger; Fiscal Year: 1992
    ....
  59. Targeted Liposomal Doxorubicin Delivery to Leukemia
    Robert Lee; Fiscal Year: 2007
    ..has been found to be non-functional, whereas the receptor expressed by KG-1 AML cells and FR-beta-transfected CHO cells mediates selective uptake and cytotoxicity of folate-coated liposomes...
  60. p120 catenin/VE-cadherin interaction in angiogenesis
    Andres Ferber; Fiscal Year: 2005
    ..We will analyze the growth characteristics of CHO cells, devoid of cadherin, transfected with either wild type or mutant VE- cadherin unable to bind p120ctn...
  61. Increased production of protein from senescent cells
    Thomas Primiano; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  62. INCREASE/gp120 IMMUNOGENICITY/LINKED ALPHA-GAL EPITOPES
    Uri Galili; Fiscal Year: 2005
    ..We propose to convert the multiple sialic acid (SA) units on recombinant gp120 produced in CHO cells, into alpha-gal epitopes (referred to as gpl20(gal) by the use of recombinant alpha1,3 galactosyltransferase (..
  63. MHCL RESTRICTED TCR RECOGNITION--STRUCTURAL BASIS
    Jia huai Wang; Fiscal Year: 2002
    ..2.8.1 CHO cells which synthesize homogeneous gylcans; a leucine zipper is used to facilitate a-b chain association...
  64. DEVELOPMENT OF CFTR AS A THERAPEUTIC PROTEIN
    Alan Smith; Fiscal Year: 1991
    ..Successful completion of the experiments will allow initiation of animal experiments and refinement of the CFTR protein delivery system...