caco 2 cells

Summary

Summary: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.

Top Publications

  1. ncbi Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria
    M Rescigno
    Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano, Italy
    Nat Immunol 2:361-7. 2001
  2. ncbi Nramp2 expression is associated with pH-dependent iron uptake across the apical membrane of human intestinal Caco-2 cells
    S Tandy
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
    J Biol Chem 275:1023-9. 2000
  3. ncbi In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli
    P K Gopal
    New Zealand Dairy Research Institute, Palmerston North
    Int J Food Microbiol 67:207-16. 2001
  4. ncbi Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD
    Annette F Baas
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Cell 116:457-66. 2004
  5. pmc Bovine prion is endocytosed by human enterocytes via the 37 kDa/67 kDa laminin receptor
    Etienne Morel
    UMR505 INSERM UPMC, 15 rue de l Ecole de Medecine, 75006 Paris, France
    Am J Pathol 167:1033-42. 2005
  6. pmc Inhibition of cyclooxygenase activity reduces rotavirus infection at a postbinding step
    John W A Rossen
    Laboratory of Pediatrics, Erasmus MC Sophia Children s Hospital, Rotterdam, The Netherlands
    J Virol 78:9721-30. 2004
  7. ncbi Binding of Clostridium difficile to Caco-2 epithelial cell line and to extracellular matrix proteins
    Marina Cerquetti
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    FEMS Immunol Med Microbiol 32:211-8. 2002
  8. pmc Giardia lamblia-induced changes in gene expression in differentiated Caco-2 human intestinal epithelial cells
    Katarina Roxström-Lindquist
    Department of Parasitology, Microbiology and Tumor Biology Center, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Infect Immun 73:8204-8. 2005
  9. ncbi Invasive Escherichia coli are a feature of Crohn's disease
    Maiko Sasaki
    Internal Medicine, Division of Digestive Diseases, Emory University, Atlanta, GA 30322, USA
    Lab Invest 87:1042-54. 2007
  10. pmc The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes
    Erin C Gaynor
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    J Bacteriol 186:503-17. 2004

Detail Information

Publications255 found, 100 shown here

  1. ncbi Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria
    M Rescigno
    Department of Biotechnology and Bioscience, University of Milano-Bicocca, Milano, Italy
    Nat Immunol 2:361-7. 2001
    ..In addition, because DCs express tight-junction proteins such as occludin, claudin 1 and zonula occludens 1, the integrity of the epithelial barrier is preserved...
  2. ncbi Nramp2 expression is associated with pH-dependent iron uptake across the apical membrane of human intestinal Caco-2 cells
    S Tandy
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, United Kingdom
    J Biol Chem 275:1023-9. 2000
    ..Taken together, these data suggest that the expression of the Nramp2 transporter in human enterocytes may play an important role in intestinal iron absorption...
  3. ncbi In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli
    P K Gopal
    New Zealand Dairy Research Institute, Palmerston North
    Int J Food Microbiol 67:207-16. 2001
    ....
  4. ncbi Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD
    Annette F Baas
    Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Cell 116:457-66. 2004
    ..We conclude that LKB1 can induce complete polarity in intestinal epithelial cells. In contrast to current thinking on polarization of simple epithelia, these cells can fully polarize in the absence of junctional cell-cell contacts...
  5. pmc Bovine prion is endocytosed by human enterocytes via the 37 kDa/67 kDa laminin receptor
    Etienne Morel
    UMR505 INSERM UPMC, 15 rue de l Ecole de Medecine, 75006 Paris, France
    Am J Pathol 167:1033-42. 2005
    ..Altogether, our results underscore a potential role of enterocytes in the absorption of bovine prions during oral infection through specific LRP/LR-dependent endocytosis...
  6. pmc Inhibition of cyclooxygenase activity reduces rotavirus infection at a postbinding step
    John W A Rossen
    Laboratory of Pediatrics, Erasmus MC Sophia Children s Hospital, Rotterdam, The Netherlands
    J Virol 78:9721-30. 2004
    ..MAPK and NF-kappaB pathways are involved in rotavirus infection but in a PGE2-independent manner. This report offers new perspectives in the search for therapeutic agents in treatment of severe rotavirus-mediated diarrhea in children...
  7. ncbi Binding of Clostridium difficile to Caco-2 epithelial cell line and to extracellular matrix proteins
    Marina Cerquetti
    Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Rome, Italy
    FEMS Immunol Med Microbiol 32:211-8. 2002
    ..Furthermore, binding of C. difficile C253 to several extracellular matrix proteins in vitro was demonstrated by an ELISA-based assay...
  8. pmc Giardia lamblia-induced changes in gene expression in differentiated Caco-2 human intestinal epithelial cells
    Katarina Roxström-Lindquist
    Department of Parasitology, Microbiology and Tumor Biology Center, Karolinska Institutet, SE 17177 Stockholm, Sweden
    Infect Immun 73:8204-8. 2005
    ..These findings indicate that the intestinal epithelium senses the G. lamblia infection, and this is important for induction of innate and adaptive immunity. The induced stress response can be important in the pathogenesis...
  9. ncbi Invasive Escherichia coli are a feature of Crohn's disease
    Maiko Sasaki
    Internal Medicine, Division of Digestive Diseases, Emory University, Atlanta, GA 30322, USA
    Lab Invest 87:1042-54. 2007
    ..coli are more prevalent in CD, are highly invasive, and do not encode for known effector proteins. E. coli strains from IBD patients regulate cytokine expression and epithelial barrier function, two pathological features of IBD...
  10. pmc The genome-sequenced variant of Campylobacter jejuni NCTC 11168 and the original clonal clinical isolate differ markedly in colonization, gene expression, and virulence-associated phenotypes
    Erin C Gaynor
    Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
    J Bacteriol 186:503-17. 2004
    ..Targeted sequencing of the sigma factors revealed specific DNA differences undetected by the other genomic methods [corrected]...
  11. ncbi Cyclooxygenase regulates angiogenesis induced by colon cancer cells
    M Tsujii
    Department of Medicine, Vanderbilt University Medical Center, VA Medical Center, Nashville, Tennessee 37232, USA
    Cell 93:705-16. 1998
    ..Cyclooxygenase regulates colon carcinoma-induced angiogenesis by two mechanisms: COX-2 can modulate production of angiogenic factors by colon cancer cells, while COX-1 regulates angiogenesis in endothelial cells...
  12. pmc Downregulation of prostaglandin E receptor subtype EP3 during colon cancer development
    Y Shoji
    Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Tokyo, Japan
    Gut 53:1151-8. 2004
    ..In the present study, we examined expression of PGE(2) receptor subtypes EP(1), EP(2), EP(3), and EP(4) in normal colon mucosa and colon cancers, and assessed the contribution of EP(3) to colon cancer development...
  13. ncbi Transport of proanthocyanidin dimer, trimer, and polymer across monolayers of human intestinal epithelial Caco-2 cells
    S Deprez
    Laboratoire de Chimie Biologique, INRA, Institut National Agronomique Paris-Grignon (INA-PG, Thiverval-Grignon, France
    Antioxid Redox Signal 3:957-67. 2001
    ..PAs, particularly the most astringent PA polymer, were also adsorbed on the epithelial cells. These results suggest that PA dimers and trimers could be absorbed in vivo and that polymer bioavailability is limited to the gut lumen...
  14. ncbi Bacterial pathogenesis: exploiting cellular adherence
    Erin C Boyle
    Department of Microbiology and Immunology, Biotechnology Laboratory, Wesbrook Building, Room 237, 6174 University Boulevard, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3
    Curr Opin Cell Biol 15:633-9. 2003
    ..Recent studies have contributed to our understanding of bacterial adherence mechanisms and the consequences of receptor engagement; they have also highlighted alternative functions of cell adhesion molecules...
  15. ncbi Mechanism of TNF-{alpha} modulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression
    Thomas Y Ma
    Internal Medicine Gastroenterology, MSC10 5550, 1 Univ of New Mexico, Albuquerque, NM 87131 0001, USA
    Am J Physiol Gastrointest Liver Physiol 288:G422-30. 2005
    ....
  16. ncbi Regional levels of drug transporters along the human intestinal tract: co-expression of ABC and SLC transporters and comparison with Caco-2 cells
    Gunilla Englund
    Department of Medical Sciences, Uppsala University, Uppsala, Sweden
    Eur J Pharm Sci 29:269-77. 2006
    ....
  17. ncbi Inhibition of Salmonella typhimurium adhesion to Caco-2 cell cultures by Lactobacillus strain GG spent culture supernate: only a pH effect?
    E M Lehto
    Department of Biochemistry and Food Chemistry, University of Turku, Finland
    FEMS Immunol Med Microbiol 18:125-32. 1997
    ..No inhibition was detected at neutral pH values. Therefore, the observed inhibition of S. typhimurium adhesion to Caco-2 monolayers with spent culture supernate I was most likely a pH effect...
  18. ncbi Protease-resistant human prion protein and ferritin are cotransported across Caco-2 epithelial cells: implications for species barrier in prion uptake from the intestine
    Ravi Shankar Mishra
    Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Neurosci 24:11280-90. 2004
    ....
  19. pmc Glutamine deprivation facilitates tumour necrosis factor induced bacterial translocation in Caco-2 cells by depletion of enterocyte fuel substrate
    E C Clark
    Gut Barrier Research Group, and Department of Surgery, University of Manchester, Hope Hospital, Manchester, UK
    Gut 52:224-30. 2003
    ..The aim of this study was to investigate the interaction between glutamine metabolism, energy supply, and inflammatory mediators in determining the translocation of non-pathogenic bacteria across cultured enterocytes...
  20. pmc Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers
    Luying Peng
    Department of Pediatrics, Mount Sinai School of Medicine, New York, NY 10029 6574, USA
    J Nutr 139:1619-25. 2009
    ..This dynamic process is mediated by the activation of AMPK. These results suggest an intriguing link between SCFA and the intracellular energy sensor for the development of the intestinal barrier...
  21. ncbi Colonic epithelial hPepT1 expression occurs in inflammatory bowel disease: transport of bacterial peptides influences expression of MHC class 1 molecules
    D Merlin
    Epithelial Pathobiology Unit, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Gastroenterology 120:1666-79. 2001
    ..hPepT1 expression normally has a strict axial gradient-highest in the proximal small intestine with no expression in the colon...
  22. pmc Conditional deletion of beta1 integrins in the intestinal epithelium causes a loss of Hedgehog expression, intestinal hyperplasia, and early postnatal lethality
    Robert G Jones
    Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    J Cell Biol 175:505-14. 2006
    ..This study shows that beta1 integrins are key regulators of proliferation and homeostasis in the intestine and achieve this not through anchorage-dependent effects but by generating Hh expression and signaling...
  23. ncbi Effect of retinoic acid on cell proliferation and differentiation as well as on lipid synthesis, lipoprotein secretion, and apolipoprotein biogenesis
    Emilie Grenier
    Department of Nutrition, Universite de Montreal, Montreal, Quebec, Canada
    Am J Physiol Gastrointest Liver Physiol 293:G1178-89. 2007
    ..Taken together, these data highlight RA-induced cell differentiation via specific signaling without a significant impact on apo A-I synthesis...
  24. pmc IL-1beta causes an increase in intestinal epithelial tight junction permeability
    Rana M Al-Sadi
    Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    J Immunol 178:4641-9. 2007
    ..The IL-1beta-induced increase in Caco-2 TJ permeability was mediated in part by the activation of NF-kappaB pathways but not apoptosis...
  25. ncbi Enteric glia inhibit intestinal epithelial cell proliferation partly through a TGF-beta1-dependent pathway
    M Neunlist
    INSERM U 539, University of Nantes, Hopital Hotel Dieu, 44035 Nantes, France
    Am J Physiol Gastrointest Liver Physiol 292:G231-41. 2007
    ..Functional alterations in EGCs may therefore modify intestinal barrier functions and be involved in pathologies such as cancer or inflammatory bowel diseases...
  26. pmc Recognition of intestinal epithelial HIF-1alpha activation by Pseudomonas aeruginosa
    Nachiket J Patel
    Department of Surgery, University of Chicago, Chicago, Illinois, USA
    Am J Physiol Gastrointest Liver Physiol 292:G134-42. 2007
    ..The ability of P. aeruginosa to metabolize adenosine to inosine may represent a subversive microbial virulence strategy that deprives the epithelium of the cytoprotective actions of adenosine...
  27. pmc Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis
    Aron B Jaffe
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    J Cell Biol 183:625-33. 2008
    ..We conclude that Cdc42 regulates epithelial tissue morphogenesis by controlling spindle orientation during cell division...
  28. pmc Butyrate induced Caco-2 cell apoptosis is mediated via the mitochondrial pathway
    F M Ruemmele
    Laboratory of Intestinal Immunology, Children s Hospital Medical Centre, Department of Paediatrics, University of Bonn, Bonn, Germany
    Gut 52:94-100. 2003
    ..The short chain fatty acid butyrate is well established as an antitumour agent which selectively induces apoptosis in colon cancer cells but not in normal intestinal epithelial cells...
  29. ncbi Inhibition of interleukin-1-stimulated NF-kappaB RelA/p65 phosphorylation by mesalamine is accompanied by decreased transcriptional activity
    L J Egan
    Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 274:26448-53. 1999
    ..These findings highlight the importance of inducible phosphorylation of RelA in the control of NF-kappaB activity...
  30. ncbi Interleukin-1beta (IL-1beta) and IL-6 modulate insulin-like growth factor-binding protein (IGFBP) secretion in colon cancer epithelial (Caco-2) cells
    M E Street
    Department of Endocrinology, St Bartholomew s Hospital, London, UK
    J Endocrinol 179:405-15. 2003
    ..These findings suggest that interleukins modulate the IGF-IGFBP system in Caco-2 cells in vitro...
  31. pmc Adhesive properties of Enterobacter sakazakii to human epithelial and brain microvascular endothelial cells
    Jean Philippe Mange
    Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, CH 8057 Zurich, Switzerland
    BMC Microbiol 6:58. 2006
    ..Furthermore, the effects of bacterial culture conditions on the adherence behaviour were investigated. An attempt was made to characterize the factors involved in adherence...
  32. ncbi Caco-2 monolayers in experimental and theoretical predictions of drug transport
    P Artursson
    Department of Pharmaceutics, Uppsala Biomedical Centre, Uppsala University, Box 580, S-751 23, Uppsala, Sweden
    Adv Drug Deliv Rev 46:27-43. 2001
    ....
  33. pmc Regulation of lactase-phlorizin hydrolase gene expression by the caudal-related homoeodomain protein Cdx-2
    J T Troelsen
    Department of Medical Biochemistry and Genetics, Biochemical Laboratory C, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    Biochem J 322:833-8. 1997
    ..A mutation in CE-LPH1, which does not affect Cdx-2 binding, results in a higher transcriptional activity, indicating that the CE-LPH1 site contains other binding site(s) in addition to the Cdx-2-binding site...
  34. ncbi FGF18 in colorectal tumour cells: autocrine and paracrine effects
    Gudrun Sonvilla
    Department of Medicine I, Institute of Cancer Research, Medical University Vienna, Vienna, Austria
    Carcinogenesis 29:15-24. 2008
    ..Together, these data strongly support an oncogenic role of FGF18 in colorectal cancer...
  35. ncbi Uptake of quercetin and quercetin 3-glucoside from whole onion and apple peel extracts by Caco-2 cell monolayers
    Jeanelle Boyer
    Departments of Food Science and Animal Science, Cornell University, Ithaca, New York 14853 7201, USA
    J Agric Food Chem 52:7172-9. 2004
    ..This use of the Caco2 cell model appears to be a simple and useful system for studying bioavailability of whole food phytochemicals and may be used to assess differences in bioavailability between foods...
  36. ncbi Human ENS regulates the intestinal epithelial barrier permeability and a tight junction-associated protein ZO-1 via VIPergic pathways
    Michel Neunlist
    Institut National de la Santé et de la Recherche Médicale U 539, Faculte de Medecine, 1, rue Gaston Veil, 44035 Nantes Cedex, France
    Am J Physiol Gastrointest Liver Physiol 285:G1028-36. 2003
    ..In conclusion, our results strongly suggest a modulatory role of VIPergic submucosal neuronal pathways on intestinal epithelial barrier permeability and ZO-1 expression...
  37. ncbi Activation of human meprin-alpha in a cell culture model of colorectal cancer is triggered by the plasminogen-activating system
    Sandra Rösmann
    Institute of Biochemistry and Molecular Biology, Department of Pediatrics, University of Berne, Buhlstrasse 28, CH 3012 Berne, Switzerland
    J Biol Chem 277:40650-8. 2002
    ..This activation mechanism offers an explanation for the observed meprin-alpha activity in the tumor stroma, a prerequisite for a potential role of this protease in colorectal cancer...
  38. ncbi Integrin alpha5/beta1 expression mediates HER-2 down-regulation in colon cancer cells
    Scott K Kuwada
    Department of Medicine, Salt Lake City Veterans Administration Health Care System and Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA
    J Biol Chem 280:19027-35. 2005
    ..These results also suggest that a novel function for integrin alpha5/beta1 is the control of HER-2 expression...
  39. ncbi Ursodeoxycholic acid differentially affects three types of sphingomyelinase in human colon cancer Caco 2 cells
    Fuli Liu
    Gastroenterology Lab, Biomedical Centre, B11, Lund University, S 22184 Lund, Sweden
    Cancer Lett 235:141-6. 2006
    We investigated the effects of UDCA on sphingomyelinase (SMase) in Caco 2 cells cultured in monolayer and polarized conditions...
  40. pmc Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes
    Jens B Andersen
    Danish Institute for Food and Veterinary Research, Department of Microbiological Food Safety, Mørkhøj Bygade 19, 2860 Søborg, Denmark
    BMC Microbiol 6:86. 2006
    ..One way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. This, however, requires invasion-neutral markers that enable easy discrimination between the different strains...
  41. ncbi Modulation of lipid synthesis, apolipoprotein biogenesis, and lipoprotein assembly by butyrate
    Valérie Marcil
    Department of Nutrition, Centre de Recherche, Hopital Sainte Justine, Universite de Montreal, Quebec, Canada H3T 1C5
    Am J Physiol Gastrointest Liver Physiol 283:G340-6. 2002
    ..05) and A-I (32%; P < 0.01). Collectively, our data indicate that butyrate may influence lipid metabolism in Caco-2 cells, thus suggesting a potential regulation of intestinal fat absorption and circulating lipoprotein concentrations...
  42. ncbi Phosphorylation of the homeotic tumor suppressor Cdx2 mediates its ubiquitin-dependent proteasome degradation
    Isabelle Gross
    Development and Physiopathology of the Intestine and Pancreas, 3, Avenue Moliere, 67200 Strasbourg, France
    Oncogene 24:7955-63. 2005
    ..Thus, our data highlight a novel post-translational mechanism controlling Cdx2 degradation via phosphorylation and polyubiquitination, which may be of importance for intestinal development and cancer...
  43. ncbi Mechanisms of epithelial translocation of the alpha(2)-gliadin-33mer in coeliac sprue
    M Schumann
    Klinik fur Gastroenterologie, Charite, Campus Benjamin Franklin, 12200 Berlin, Germany
    Gut 57:747-54. 2008
    ..The alpha(2)-gliadin-33mer has been shown to be important in the pathogenesis of coeliac disease. We aimed to study mechanisms of its epithelial translocation and processing in respect to transcytotic and paracellular pathways...
  44. pmc POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP
    Peixiang Wang
    Division of Cell and Molecular Biology, Toronto General Research Institute, University Health Network, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 284:26456-65. 2009
    ..Our study therefore identifies a novel mechanism by which cAMP regulates hormone-gene expression and suggests that ubiquitously expressed Oct-1 may play a role in metabolic homeostasis by functioning as a sensor for cAMP...
  45. ncbi Epigenetic properties of the diarrhetic marine toxin okadaic acid: inhibition of the gap junctional intercellular communication in a human intestine epithelial cell line
    Adama Traore
    Laboratory of Toxicology and Applied Hygiene, University Victor Segalen Bordeaux 2, 146, rue Leo Saignat, 33076, Bordeaux, France
    Arch Toxicol 77:657-62. 2003
    ..These data are discussed in relation to the toxicity of OA, total RNA synthesis, and possible specificity of Cx 43 inhibition in the GJIC...
  46. ncbi A co-clustering model involving alpha5beta1 integrin for the biological effects of GPI-anchored human carcinoembryonic antigen (CEA)
    Pilar Camacho-Leal
    Department of Biochemistry and McGill Cancer Centre, McGill University, Quebec, Canada
    J Cell Physiol 211:791-802. 2007
    ..These changes may represent a molecular mechanism for the biological effects of CEA...
  47. ncbi Arginine deficiency in preconfluent intestinal Caco-2 cells modulates expression of proteins involved in proliferation, apoptosis, and heat shock response
    Kaatje Lenaerts
    Maastricht Proteomics Center, Nutrition and Toxicology Research Institute Maastricht NUTRIM, Department of Human Biology, Maastricht University, Maastricht, The Netherlands
    Proteomics 7:565-77. 2007
    ..These processes are critical for proper cell function, and hence a state of arginine deficiency can be detrimental for intestinal cells which proliferate actively in vivo...
  48. ncbi Regulation of sterol regulatory element-binding proteins by cholesterol flux in CaCo-2 cells
    F J Field
    Department of Internal Medicine, 4 522 JCP, 200 Hawkins Drive, and Veterans Administration, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Lipid Res 42:1687-98. 2001
    ..In contrast, 25-hydroxycholesterol influx or cholesterol depletion alters the amount of mature SREBP, leading to the regulation of sterol-responsive gene expression...
  49. ncbi Assessment of carotenoid bioavailability of whole foods using a Caco-2 cell culture model coupled with an in vitro digestion
    Chang Shu Liu
    Department of Food Science and Institute of Comparative and Environmental Toxicology, Stocking Hall, Cornell University, Ithaca, New York 14853, USA
    J Agric Food Chem 52:4330-7. 2004
    ..These results support the feasibility of the in vitro digestion cell model for assessing carotenoid absorption from whole foods as a suitable and cost-effective physiological alternative to current methodologies...
  50. ncbi Transport characteristics of a novel peptide transporter 1 substrate, antihypotensive drug midodrine, and its amino acid derivatives
    Masahiro Tsuda
    Department of Pharmacy, Kyoto University Hospital, Sakyo ku, Kyoto 606 8507, Japan
    J Pharmacol Exp Ther 318:455-60. 2006
    ..These findings indicate that PEPT1 can transport midodrine and contributes to the high bioavailability of this drug and that Gly modification of DMAE is desirable for a prodrug of DMAE...
  51. ncbi Disruption of hedgehog signaling reveals a novel role in intestinal morphogenesis and intestinal-specific lipid metabolism in mice
    Li Chun Wang
    Department of Exploratory Science, Biogen Inc, Cambridge, Massachusetts 02142, USA
    Gastroenterology 122:469-82. 2002
    ..The hedgehog (hh) signaling pathway has been shown to play crucial roles in the development of embryonic gut. However, its role in intestinal development and function beyond the embryonic stage is still undefined...
  52. pmc Both thiamine uptake and biosynthesis of thiamine precursors are required for intracellular replication of Listeria monocytogenes
    Kristina Schauer
    Zentralinstitut für Ernährungs und Lebensmittelforschung, Abteilung Mikrobiologie, Technische Universitat Munchen, Weihenstephaner Berg, Freising, Germany
    J Bacteriol 191:2218-27. 2009
    ..monocytogenes and possibly other facultative intracellular pathogens to enable proliferation within the cytoplasm...
  53. ncbi Proteome analysis reveals novel proteins associated with proliferation and differentiation of the colorectal cancer cell line Caco-2
    Rob Stierum
    TNO Nutrition and Food Research, Department of Biomolecular Sciences, P O Box 360, 3700 AJ, Zeist, The Netherlands
    Biochim Biophys Acta 1650:73-91. 2003
    ..These findings illustrate that a combination of proteomics and cell culture is a useful approach to find markers for Caco-2 differentiation, which could contribute to the comprehension of the process of colon carcinogenesis...
  54. ncbi The motogenic effects of cyclic mechanical strain on intestinal epithelial monolayer wound closure are matrix dependent
    Jianhu Zhang
    Department of Surgery, John D Dingell VA Medical Center, 4646 John R Street, Detroit, MI 48301, USA
    Gastroenterology 131:1179-89. 2006
    ....
  55. ncbi Expression of thirty-six drug transporter genes in human intestine, liver, kidney, and organotypic cell lines
    Constanze Hilgendorf
    Discovery Drug Metabolism and Pharmacokinetics and Bioanalytical Chemistry, AstraZeneca Research and Development, Molndal, Sweden
    Drug Metab Dispos 35:1333-40. 2007
    ....
  56. pmc Application of mutated Clostridium difficile toxin A for determination of glucosyltransferase-dependent effects
    Matthias Teichert
    Institut fur Toxikologie, Medizinische Hochschule Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany
    Infect Immun 74:6006-10. 2006
    ..The mutant toxin A was characterized and applied to distinguish between glucosyltransferase-dependent and -independent effects with respect to RhoB up-regulation as a cellular stress response...
  57. ncbi Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions
    Carolyn B Coyne
    Division of Infectious Diseases, The Children s Hospital of Philadelphia, University of Pennsylvania, PA 19104, USA
    Cell 124:119-31. 2006
    ..CVBs thus exploit DAF-mediated signaling pathways to surmount the epithelial barrier...
  58. pmc Anti-inflammatory effects of phosphatidylcholine
    Irina Treede
    Department of Gastroenterology, University Hospital Heidelberg, INF 345, Heidelberg 69120, Germany
    J Biol Chem 282:27155-64. 2007
    ..Collectively, these results provide a molecular foundation for the clinical studies showing a beneficial effect of PC therapy in ulcerative colitis...
  59. pmc Sigma B contributes to Listeria monocytogenes gastrointestinal infection but not to systemic spread in the guinea pig infection model
    M R Garner
    Department of Food Science, 413 Stocking Hall, Cornell University, Ithaca, NY 14853, USA
    Infect Immun 74:876-86. 2006
    ....
  60. pmc Proteasome-mediated degradation of IkappaBalpha and processing of p105 in Crohn disease and ulcerative colitis
    Alexander Visekruna
    Max Planck Institute of Infection Biology, Berlin, Germany
    J Clin Invest 116:3195-203. 2006
    ..These findings suggest that distinct proteasome subunits influence the intensity of NF-kappaB-mediated inflammation in IBD patients...
  61. pmc Gp96 is a receptor for a novel Listeria monocytogenes virulence factor, Vip, a surface protein
    Didier Cabanes
    Unité des Interactions Bactéries Cellules Institut Pasteur, INSERM U604, INRA USC 2020, Paris, France
    EMBO J 24:2827-38. 2005
    ..Vip thus appears as a new virulence factor exploiting Gp96 as a receptor for cell invasion and/or signalling events that may interfere with the host immune response in the course of the infection...
  62. ncbi Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli
    Yuan Kun Lee
    Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    J Med Microbiol 52:925-30. 2003
    ..These findings are important for development, selection and in vitro assessment of target- and function-specific probiotics...
  63. ncbi Norwalk virus binds to histo-blood group antigens present on gastroduodenal epithelial cells of secretor individuals
    Severine Marionneau
    INSERM U419, Institut de Biologie, Nantes, France
    Gastroenterology 122:1967-77. 2002
    ..Our aim was to test if, by analogy, recombinant NV-like particles (rNV VLPs) use carbohydrates present on human gastroduodenal epithelial cells as ligands...
  64. pmc Flagellin-dependent and -independent inflammatory responses following infection by enteropathogenic Escherichia coli and Citrobacter rodentium
    Mohammed A Khan
    Division of Gastroenterology, BC Children s Hospital, Vancouver, British Columbia, Canada
    Infect Immun 76:1410-22. 2008
    ....
  65. pmc Hypoxia-inducible factor 1-dependent induction of intestinal trefoil factor protects barrier function during hypoxia
    G T Furuta
    Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Exp Med 193:1027-34. 2001
    ..HIF-1-dependent induction of ITF may provide an adaptive link for maintenance of barrier function during hypoxia...
  66. pmc NF-kappaB- and AP-1-mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917: a novel effect of a probiotic bacterium
    Jan Wehkamp
    Department of Internal Medicine I, Robert Bosch Hospital, Stuttgart, Germany
    Infect Immun 72:5750-8. 2004
    ..The induction of hBD-2 may contribute to an enhanced mucosal barrier to the luminal bacteria...
  67. ncbi CARD15/NOD2 functions as an antibacterial factor in human intestinal epithelial cells
    Tadakazu Hisamatsu
    Gastrointestinal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Gastroenterology 124:993-1000. 2003
    ..Because intestinal epithelial cells play a role as the barrier to luminal microorganisms, we investigated the expression and function of CARD15/NOD2 in intestinal epithelial cells...
  68. ncbi Specific 1,25(OH)2D3-mediated regulation of transcellular calcium transport in Caco-2 cells
    J C Fleet
    Department of Nutrition and Foodservice Systems, University of North Carolina at Greensboro, Greensboro, North Carolina 27402, USA
    Am J Physiol 276:G958-64. 1999
    ..These data demonstrate that calcitriol-mediated A-to-B calcium transport in Caco-2 cells is a specific, transcellular process that requires transcriptional events normally mediated through the vitamin D receptor...
  69. pmc Probiotics ameliorate the hydrogen peroxide-induced epithelial barrier disruption by a PKC- and MAP kinase-dependent mechanism
    A Seth
    Dept of Physiology, Univ of Tennessee Health Science Center, Memphis, TN 38163, USA
    Am J Physiol Gastrointest Liver Physiol 294:G1060-9. 2008
    ..These studies demonstrate that probiotic-secretory proteins protect the intestinal epithelial tight junctions and the barrier function from hydrogen peroxide-induced insult by a PKC- and MAP kinase-dependent mechanism...
  70. pmc Enhanced synthesis of internalin A in aro mutants of Listeria monocytogenes indicates posttranscriptional control of the inlAB mRNA
    Jochen Stritzker
    Biocenter Microbiology, University of Wurzburg, Am Hubland, Wurzburg, Germany
    J Bacteriol 187:2836-45. 2005
    ..The latter mode of control seemed to be related to the predominantly anaerobic metabolism of the aro mutants...
  71. pmc Growth factor-like activity of gliadin, an alimentary protein: implications for coeliac disease
    Maria Vittoria Barone
    Dipartimento di Pediatria European Laboratory For the Investigation of Food Induced Disease, Universita degli Studi di Napoli Federico II, CAP 80131, Napoli, Italy
    Gut 56:480-8. 2007
    ..In addition to 'immunogenic' effects, gliadin directly affects cultured cells and intestine preparations, and produces damage in vivo, via a separate 'toxic' peptide, such as A-gliadin p31-43 (P31-43)...
  72. ncbi Predicting drug absorption from molecular surface properties based on molecular dynamics simulations
    L H Krarup
    Department of Pharmaceutics, Royal Danish School of Pharmacy, Copenhagen
    Pharm Res 15:972-8. 1998
    ....
  73. ncbi Iron uptake is enhanced in Caco-2 cell monolayers by cysteine and reduced cysteinyl glycine
    R P Glahn
    U S Plant, Soil and Nutrition Laboratory, U S Department of Agriculture Agricultural Research Service, Ithaca, NY 14853, USA
    J Nutr 127:642-7. 1997
    ..The similarity between this study and human studies further reinforces that the Caco-2 cell model is a useful tool in studies of iron absorption and bioavailability...
  74. ncbi The alpha2 and alpha3 integrins are required for morphologic differentiation of an intestinal epithelial cell line
    Xihong Zhang
    University of Minnesota Cancer Center, Department of Surgery, University of Minnesota, Minneapolis, MN, USA
    Surgery 133:429-37. 2003
    ..We have taken advantage of the ability of the Caco-2 cell line to acquire a glandular phenotype in 3-dimensional (3-D) culture systems to investigate the role of alpha2 and alpha3 integrins in morphologic differentiation...
  75. ncbi Multiple transcription factors regulating the IL-6 gene are activated by cAMP in cultured Caco-2 cells
    Dan D Hershko
    Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45267, USA
    Am J Physiol Regul Integr Comp Physiol 283:R1140-8. 2002
    ....
  76. ncbi EPS15R, TASP1, and PRPF3 are novel disease candidate genes targeted by HNF4alpha splice variants in hepatocellular carcinomas
    Monika Niehof
    Fraunhofer Institute of Toxicology and Experimental Medicine, Center of Drug Research and Medical Biotechnology, Hannover, Germany
    Gastroenterology 134:1191-202. 2008
    ..The role of HNF4alpha splice variants in hepatocellular carcinomas (HCC) is unknown. Here, we report an identification of novel candidate genes targeted by splice variants of HNF4alpha...
  77. ncbi pH dependent uptake of loperamide across the gastrointestinal tract: an in vitro study
    Andrew Crowe
    School of Pharmacy, Curtin University of Technology, Perth, Western Australia
    Drug Dev Ind Pharm 30:449-59. 2004
    ..Hence, our aim was to determine the influence of pH gradient conditions on the gastrointestinal uptake of loperamide, including any changes to its P-gp-mediated efflux...
  78. ncbi Osmotic stress, a proinflammatory signal in Caco-2 cells
    Aurelie Hubert
    Aden, EA 3234, IFR 23, UFR Médecine Pharmacie de Rouen, 22, Bd Gambetta, 76183 Rouen, France
    Biochimie 86:533-41. 2004
    ..Taken together, these results demonstrate that osmotic stress is a proinflammatory signal in Caco-2 cells and suggest that an osmosensor might specifically exist in intestinal epithelial cells...
  79. ncbi Components of intestinal epithelial hypoxia activate the virulence circuitry of Pseudomonas
    Jonathan E Kohler
    Department of Surgery, University of Chicago, Chicago, Illinois 60637, USA
    Am J Physiol Gastrointest Liver Physiol 288:G1048-54. 2005
    ..We conclude that the virulence circuitry of P. aeruginosa is activated by both soluble and contact-mediated elements of the intestinal epithelium during hypoxia and normoxic recovery...
  80. ncbi LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3
    Linda C H Yu
    Department of Biological Sciences, BI 117 Mucosal Inflammation Research Group, University of Calgary, 2500 University Dr N W, Calgary, AB, T2N 1N4, Canada
    Exp Cell Res 312:3276-86. 2006
    ..The understanding of these signaling events may shed light on enterocytic cytoprotection and homeostasis mechanism upon pro-apoptotic challenges...
  81. ncbi Probiotics potentiate IL-6 production in IL-1beta-treated Caco-2 cells through a heat shock-dependent mechanism
    Natasha Reilly
    Department of Surgery, Beth Israel Deaconess Medical Center, 330 Brookline Ave, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 293:R1169-79. 2007
    ..Results suggest that probiotics may enhance IL-6 production in enterocytes subjected to an inflammatory stimulus and that this effect may, at least in part, be heat shock dependent...
  82. ncbi Polyunsaturated fatty acids down-regulate in vitro expression of the key intestinal cholesterol absorption protein NPC1L1: no effect of monounsaturated nor saturated fatty acids
    Adriana Alvaro
    Research Unit on Lipids and Atherosclerosis, Sant Joan University Hospital, University Rovira and Virgili, Reus, CIBER de Diabetes y Enfermedades Metabólicas Asociadas, IISPV, Spain
    J Nutr Biochem 21:518-25. 2010
    ..LXR and RXR activation induced a similar repression effect. The lipid-lowering effect of n-3 PUFAs could be mediated in part by their action at the NPC1L1 gene level...
  83. ncbi Flavonoid structure affects the inhibition of lipid peroxidation in Caco-2 intestinal cells at physiological concentrations
    I Wen Peng
    Department of Exercise and Nutrition Sciences, University at Buffalo, Buffalo, NY 14214, USA
    J Nutr 133:2184-7. 2003
    ..1-10 micro mol/L increased H(2)O(2) and Fe(2+)-induced lipid peroxidation after co- or preincubation. In summary, these observations support the importance of plant-based food items such as vegetables, fruits and teas in the diet...
  84. ncbi Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion
    Sumbul Fatma
    Department of Anatomy and Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
    J Lipid Res 47:2422-32. 2006
    ..They inhibit fat absorption by decreasing TG transport to the endoplasmic reticulum and increase drug efficacy against cancer cells by competing for their excretion...
  85. ncbi Epithelial-derived fibronectin expression, signaling, and function in intestinal inflammation
    Vasantha L Kolachala
    Division of Digestive Diseases, Department of Medicine, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    J Biol Chem 282:32965-73. 2007
    ....
  86. ncbi Repressed E-cadherin expression in the lower crypt of human small intestine: a cell marker of functional relevance
    Fabrice Escaffit
    Département d Anatomie et de Biologie Cellulaire, Faculte de Medecine, Universite de Sherbrooke, CIHR Group in Functional Development and Physiopathology of the Digestive Tract, Sherbrooke, Quebec, Canada J1H 5N4
    Exp Cell Res 302:206-20. 2005
    ..These results suggest that down-regulated expression of E-cadherin is a human intestinal crypt base cell-related feature that appears to be of functional relevance for the maintenance of the progenitor cell population...
  87. ncbi Transcriptome variations in human CaCo-2 cells: a model for enterocyte differentiation and its link to iron absorption
    Hélène Bédrine-Ferran
    UMR 6061 CNRS, IFR 97, Faculte de Medecine, CS 34317 35043 Rennes, France
    Genomics 83:772-89. 2004
    ....
  88. ncbi High-molecular-weight polyethylene glycol prevents lethal sepsis due to intestinal Pseudomonas aeruginosa
    Licheng Wu
    Department of Surgery, University of Chicago, IL 60637, USA
    Gastroenterology 126:488-98. 2004
    ..The aims of this study were to test the ability of a high-molecular-weight polyethylene glycol compound, polyethylene glycol 15-20, to protect the intestinal epithelium against microbial invasion during stress...
  89. ncbi Arachidonic acid cascade and epithelial barrier function during Caco-2 cell differentiation
    Raquel Martín-Venegas
    Departament de Fisiologia, Facultat de Farmacia, Universitat de Barcelona, 08028 Barcelona, Spain
    J Lipid Res 47:1416-23. 2006
    ..These results suggest that AA release by calcium-independent PLA(2), COX-2 expression, and subsequent PGE(2) release are important for the maintenance of paracellular permeability in differentiated Caco-2 cells...
  90. pmc Effects of grapefruit juice and orange juice components on P-glycoprotein- and MRP2-mediated drug efflux
    Yoko Honda
    Department of Medico Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Br J Pharmacol 143:856-64. 2004
    ..The present study has revealed that GFJ and OJ interact with not only P-gp but also MRP2, both of which are expressed at apical membranes and limit the apical-to-basal transport of VBL and saquinavir in Caco-2 cells...
  91. ncbi Glycoprofiling with micro-arrays of glycoconjugates and lectins
    S Angeloni
    CSEM S A, Centre Suisse d Electronique et de Microtechnique, rue Jaquet Droz 1, CH 2000 Neuchatel, Switzerland
    Glycobiology 15:31-41. 2005
    ..All immobilized glycans were specifically and differentially detected either on glycoconjugate or lectin arrays. The results demonstrate the feasibility and versatility of the novel platforms for glycan profiling...
  92. ncbi Roles of ZO-1, occludin, and actin in oxidant-induced barrier disruption
    Mark W Musch
    The University of Chicago Hospitals, 5841 S Maryland Ave, MC 6084, Chicago, IL 60637, USA
    Am J Physiol Gastrointest Liver Physiol 290:G222-31. 2006
    ..These effects are likely to contribute to the development of malabsorption and dysfunction associated with mucosal inflammation of the digestive tract...
  93. ncbi Endogenous production of leukotriene D4 mediates autocrine survival and proliferation via CysLT1 receptor signalling in intestinal epithelial cells
    S Paruchuri
    Cell and Experimental Pathology, Department of Laboratory Medicine, Lund University, Malmo University Hospital, Malmo, Sweden
    Oncogene 25:6660-5. 2006
    ..Importantly, we propose that tumour-derived intestinal cells are resistant to CysLT1R antagonist-induced apoptosis, a phenomena that could be explained by nuclear CysLT1R signalling...
  94. ncbi Virulence of Enterococcus isolates collected in Lower Silesia (Poland)
    Ewa Dworniczek
    Department of Microbiology, Medical University of Wrocław, Poland
    Scand J Infect Dis 37:630-6. 2005
    ..4%, biofilm in 45.3%, haemolysins in 15.5%, DNAse in 12.2% and lipase in 33.1% of enterococcal isolates. It appears that the adherence of the enterococci studied was not significantly associated with the presence of virulence factors...
  95. ncbi Epitopes in the P2 domain of norovirus VP1 recognized by monoclonal antibodies that block cell interactions
    Vance P Lochridge
    Veterinary Molecular Biology Laboratory, Montana State University, PO Box 173610, Bozeman, MT 59717, USA
    J Gen Virol 86:2799-806. 2005
    ..This study identifies the first epitope involved in inhibition of norovirus-cell interactions and supports increasing evidence that interactions between noroviruses and host cells rely on structures in the P2 domain of VP1...
  96. pmc Increased basolateral sorting of carcinoembryonic antigen in a polarized colon carcinoma cell line after cholesterol depletion-Implications for treatment of inflammatory bowel disease
    Robert Ehehalt
    Department of Internal Medicine IV University Hospital, INF410, Heidelberg 69120, Germany
    World J Gastroenterol 14:1528-33. 2008
    ..To investigate a possible increase of basolateral expression of carcinoembryonic antigen (CEA) by interfering with the apical transport machinery, we studied the effect of cholesterol depletion on CEA sorting and secretion...
  97. ncbi Evaluation of biotransformation of sulphasalazine in the colon epithelial Caco-2 cells
    Beata Bat
    Department of Biochemistry, Medical University of Silesia, 1 Narcyzów Str, 41 200 Sosnowiec, Poland
    Acta Pol Pharm 61:8-10. 2004
    ..The other metabolite of SAS i.e., SP, was not transformed in the human colon cancer cells at all...
  98. ncbi Comparative study of glycosyltransferase activities in Caco-2 cells before and after enterocytic differentiation using lectin-affinity high-performance liquid chromatography
    J Amano
    Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113 0033, Japan
    Arch Biochem Biophys 395:191-8. 2001
    ..These results demonstrate that an increase in specific glycosyltransferase activities brought on a change of the O-glycan structures during differentiation...
  99. ncbi Ochratoxin A secretion by ATP-dependent membrane transporters in Caco-2 cells
    Jan Schrickx
    Department of Veterinary Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 16, 3584 CM, Utrecht, The Netherlands
    Arch Toxicol 80:243-9. 2006
    ..These findings are of interest in evaluating mycotoxin absorption after oral ingestion, tissue distribution and particularly excretion pathways, including renal, biliary and mammary gland excretion...
  100. ncbi PPARgamma inhibitors reduce tubulin protein levels by a PPARgamma, PPARdelta and proteasome-independent mechanism, resulting in cell cycle arrest, apoptosis and reduced metastasis of colorectal carcinoma cells
    Katherine L Schaefer
    Section of Gastroenterology and Hepatology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Int J Cancer 120:702-13. 2007
    ..These results suggest that PPARgamma inhibitors are a novel potential antimicrotubule therapy for CRC that acts by directly reducing microtubule precursors...
  101. ncbi Rapid activation of Na+/H+ exchange by EPEC is PKC mediated
    Kim Hodges
    Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois, Chicago, IL 60612, USA
    Am J Physiol Gastrointest Liver Physiol 291:G959-68. 2006
    ..Because EPEC markedly increases NHE2 activity, this pathogen provides an exceptional opportunity to improve our understanding of this less-characterized NHE isoform...

Research Grants63

  1. Gut mucosal mast cells are activated by fat absorption: physiology and mechanism
    Patrick Tso; Fiscal Year: 2013
    ..g. chylomicrons (CMs) or the media containing CMs by the intestinal epithelial cell line (Caco-2 cells) on RBC-2H3 cell NF-kB response. If the outcome is positive, we will isolate the factor/factors involved. ..
  2. CD36 and Intestinal Fat Absorption
    Nada A Abumrad; Fiscal Year: 2013
    ..We should learn whether targeting intestinal CD36 may be beneficial in disorders like obesity, insulin resistance and gallstone formation. ..
  3. Zinc inhibition of endotoxemia in alcoholic liver injury
    Zhanxiang Zhou; Fiscal Year: 2010
    ..The results would have high potential leading to a simplistic approach to prevention/treatment of ALD. ..
  4. Enhancement of NAFLD risk by vinyl chloride: interaction of gut-liver-adipose axi
    JULIANE INGEBORG BEIER; Fiscal Year: 2013
    ....
  5. Dietary Fat in Ethanol-Induced Intestinal Barrier Dysfunction in ALD
    Irina Kirpich; Fiscal Year: 2013
    ..Dietary modulation may represent a novel preventive/therapeutic approach in ALD, and MLCK could be a specific novel therapeutic target. ..
  6. Effector Functions of Mucosal IgA
    Sherie L Morrison; Fiscal Year: 2012
    ..The proposed studies will increase our understanding of how oral vaccines are taken up by the human intestine. They will also provide new strategies for the development of safe and efficient needle-free vaccines. ..
  7. Intestinal Biotin Absorption: Physiological, Cellular and Molecular Aspects
    Hamid M Said; Fiscal Year: 2013
    ..This should ultimately assist us in the designing of effective strategies to optimize biotin body homeostasis, especially in conditions associated with biotin deficiency and sub-optimal levels. ..
  8. Molecular characteristics of the apical recycling system
    JAMES RICHARD GOLDENRING; Fiscal Year: 2013
    ....
  9. SMALL GTP BINDING PROTEINS IN GASTROINTESTINAL MUCOSA
    JAMES RICHARD GOLDENRING; Fiscal Year: 2013
    ..These investigations will establish how disruption of vesicle trafficking processes that regulate the establishment and maintenance of intestinal epithelial cell polarity may lead to carcinogenesis. ! ..
  10. Transporters and Trafficking in Polarized Epithelial Cells
    Mark Donowitz; Fiscal Year: 2013
    ....
  11. The iron modulatory function of prion protein and prion disorders
    Neena Singh; Fiscal Year: 2013
    ..Successful completion of these studies will uncover novel pathway(s) of iron modulation by PrPC, and improve our understanding of the mechanism(s) underlying brain iron imbalance and associated neurotoxicity in prion disorders. ..
  12. Colorectal cancer chemoprevention with phytochemical combinations
    Swati Nagar; Fiscal Year: 2013
    ..Data from the proposed aims will form the basis of future studies to evaluate additional metabolizing enzymes and transporters that are modulated by phytochemicals. ..
  13. Rho GTPases and polarity signaling pathways in morphogenesis and migration
    Alan Hall; Fiscal Year: 2013
    ..This program of research will lead to a significant advance in understanding the control of migration and morphogenesis and provide greater insight into the underlying cell biology associated with a wide range of human disorders. ..
  14. Transporter Knockdowns for In Vitro Drug Interaction Monitoring
    ISMAEL JUVENAL HIDALGO; Fiscal Year: 2010
    ....
  15. Transcriptional control of epithelial identity during pyloric border formation
    AARON MARK UDAGER; Fiscal Year: 2010
    ..Since intestinal metaplasia is thought to represent an early step in the development of some forms of gastric cancer, our findings could eventually impact diagnosis or early treatment of this often fatal cancer. ..
  16. Compound library screening using Giardia labmblia high throughput viability assay
    Osnat Herzberg; Fiscal Year: 2012
    ..This assay will be used to screen the NIH compound library and identify compounds that kill Giardia trophozoites. ..
  17. Role of EGFR Ligands in Neoplasia
    Robert J Coffey; Fiscal Year: 2013
    ..Finally, we will test the hypothesis that EREG is a "metastasis promoter" by attempting to prevent lung metastasis in a syngeneic mouse colon cancer model through elimination of EREG. ..
  18. C/EBP and IL-6 Production in Mucosa and Enterocytes
    Per Olof Hasselgren; Fiscal Year: 2006
    ....
  19. Cellular models of microvillus inclusion disease
    H Lee Sweeney; Fiscal Year: 2013
    ..The creation of these lines will set the stage for future studies to understand the role of myosin Vb in apical membrane recycling as well as provide a better understanding of the pathogenesis of MVID. ..
  20. Function of Myosin-X in Intestinal Apical Domain Assembly
    KATY LIU; Fiscal Year: 2013
    ..The proposed experiments will further our understanding of apical domain assembly, which is a necessary first step to address apical domain dysfunction in intestinal disease. ..
  21. Structure and Function of Myosin VI
    H Lee Sweeney; Fiscal Year: 2013
    ..transport roles of myosin VI. ..
  22. NHE3 Regulation and Signaling Complexes
    Chung Ming Tse; Fiscal Year: 2013
    ..abstract_text> ..
  23. Drug-Dietary Flavonoid Intestinal Absorption Interaction
    ROSITA PROTEAU; Fiscal Year: 2002
    ..Thus, the results of this study will increase our understanding of the role of flavonoids found in tea, vegetables, soy, and dietary supplements in the intestinal absorption of therapeutic drugs. ..
  24. Ferritin and Iron Nutrition in Health and Disease
    Elizabeth Theil; Fiscal Year: 2005
    ..The results will clarify mechanisms of ferritin iron uptake and characterize molecular genetic differences in iron uptake for improving dietary iron sources in health and disease. ..
  25. TG RICH LIPOPROTEIN SYNTHESIS/SECRETION IN CACO 2 CELLS
    F Field; Fiscal Year: 2000
    ....
  26. INTESTINAL PROTEIN METABOLISM AND FUNCTION
    David Alpers; Fiscal Year: 2000
    ....
  27. Short Chain Fatty Acids and Intestinal Barrier Function
    Jing Lin; Fiscal Year: 2007
    ..The results of this study may help us to further understand the pathogenesis of NEC and lead to the development of novel prevention strategies and treatment methods. [unreadable] [unreadable] [unreadable]..
  28. Gastrin-Releasing Peptide Receptor Desensitization
    RICHARD BENYA; Fiscal Year: 2007
    ..Overall these studies will provide critical information regarding colon cancer differentiation, and shed light on a novel mechanism regulating the inactivation, or desensitization, of a clinically important heptaspanning receptor. ..
  29. Transepithelial Transport of Poly Amidoamine Dendrimers
    KELLY KITCHENS; Fiscal Year: 2007
    ..5-G4.5) in comparison to the permeability of cationic, amine-terminated dendrimers (PAMAM-NH2, G0-G4) will be used to assess the effect of surface charge on PAMAM permeability. ..
  30. INTESTINAL SPECIFIC APO B: ASSEMBLY AND PROCESSING
    VODEK SASAK; Fiscal Year: 1990
    ..This should contribute to the understanding of intestinal lipid metabolism and determination of metabolic defects in various lipid disorders...
  31. Studies of Human Folate Hydrolase
    Charles Halsted; Fiscal Year: 2005
    ..abstract_text> ..
  32. Modulation of the Intercellular Junction Cadherins
    Teruna Siahaan; Fiscal Year: 2001
    ..abstract_text> ..
  33. IN VITRO DELIVERABILITY OF ANTI-ANTHRAX DISCOVERY LEADS
    R Porter; Fiscal Year: 2000
    ..abstract_text> ..
  34. AMINOKETONE INHIBITORS OF THE DOPAMINE TRANSPORTER
    JOHN REINHARD; Fiscal Year: 2005
    ..if the ester conjugates can be taken up into caco-2 cells (by hPEPT1 and 2). If successful, Phase II money will complete the preclinical studies required for support of human clinical trials. ..
  35. Molecular Mechanisms of Intrahepatic Cholestasis
    Benjamin Shneider; Fiscal Year: 2006
    ..These innovative studies will delineate a novel set of mechanisms that underlie the pathogenesis of PFIC1 and will develop a new area of cellular regulation of lipid membrane asymmetry. ..
  36. MECHANISMS OF LEUKOCYTE RECRUITMENT IN IBD
    Ciaran Kelly; Fiscal Year: 2005
    ..An in-depth, mechanistic understanding of the regulation of ENA-78 gene expression in human enterocytes may lead to novel therapeutic approaches to IBD. ..
  37. Lead identification of 1,4-benzoxazines as anti-tuberculosis agents
    Scott Franzblau; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  38. INTESTINAL PERFUSION AND PERMEABILITY IN SEPSIS
    RUSSELL DELUDE; Fiscal Year: 2007
    ..abstract_text> ..
  39. PEPT1: STRUCTURE-FUNCTION, SORTING AND MODULATION
    IAN HAWORTH; Fiscal Year: 2003
    ..abstract_text> ..
  40. Roles of Iron Transport Factor Genes In Iron Absorption
    Okhee Han; Fiscal Year: 2006
    ..The completion of the proposed study in this R21 will be a critical step in achieving our long-term goals. ..
  41. ROLES OF PKC ISOFORMS IN COLONIC CARCINOGENESIS
    Marc Bissonnette; Fiscal Year: 2002
    ....
  42. RXR-Heterodimeric Receptors in the GI Tract
    Ellen Li; Fiscal Year: 2006
    ..We propose to study intraheterodimerization interactions between RXR and the minimal peptide by nuclear magnetic resonance techniques. ..
  43. Molecular Characterization of the SLC19A2 Promoter
    JACK REIDLING; Fiscal Year: 2005
    ..Nothing is known about possible regulation of the thiamine uptake process during development and whether this includes promoter regulation. These issues will be addressed in this proposal. ..
  44. Modulation of NHE Activity by EPEC Infection
    Pradeep Dudeja; Fiscal Year: 2006
    ..Our findings may aid in the future development of improved therapeutic modalities for infectious diarrhea. [unreadable] [unreadable] [unreadable]..
  45. NA,K-ATPASE REGULATION IN INTESTINE
    Michael Field; Fiscal Year: 1992
    ....
  46. RESVERATROL BIOAVAILABILITY--PRECLINICAL AND CLINICAL
    Thomas Walle; Fiscal Year: 2002
    ..Together Aims 1 and 2 will let us determine the usefulness of the Caco-2 cells as a preclinical model. ..
  47. INTESTINAL VITAMIM A BINDING PROTEINS
    Ellen Li; Fiscal Year: 2001
    ....
  48. MOLECULAR MECHANISM OF REGULATION OF NA/H EXCHANGER NHE3
    ANDRZEJ JANECKI; Fiscal Year: 2001
    ..We will then engineer and transfect into Caco 2 cells a fusion of NHE3 and green fluorescent protein (NHE3-GFP) cDNA, which would enable us to simultaneously ..
  49. MOLECULAR MECHANISM OF REGULATION OF NA/H EXCHANGER NHE3
    ANDRZEJ JANECKI; Fiscal Year: 2000
    ....
  50. COLONIC EPITHELIAL CELL PLASMA MEMBRANES
    Thomas Brasitus; Fiscal Year: 1999
    ....
  51. RAT INTESTINAL MEMBRANE--VITAMIN D-MEDIATED EFFECTS
    Michael Sitrin; Fiscal Year: 2001
    ..Accordingly the specific aims of the proposal are to characterize the mechanisms involved in inhibition of cellular proliferation in CaCo-2 cells and the actions of the secosteroids in inducing apoptosis. ..
  52. ASSEMBLY OF APOB48 CONTAINING LIPOPROTEINS
    M Hussain; Fiscal Year: 1999
    ..The cell lines developed will be valuable in the study of intracellular mechanisms of lipoprotein assembly. ..
  53. SOCS-3 in IGF-IR Signaling in Malignant Cells
    BHAKTA DEY; Fiscal Year: 2003
    ..Our long-term goal is to investigate the negative regulation of the IGF-IR signaling by SOCS-3, a potentially novel mechanism for growth resistance and cancer prevention. ..
  54. EGF, Oxidants & Intestinal Barrier Integrity
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  55. Protective Mechanism of an Anti-Inflammatory Herbal Mix
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  56. Roles of ErbB Signaling in Colonic Carcinogenesis
    Marc Bissonnette; Fiscal Year: 2007
    ..g., EGFR blockade by Iressa) or effectors in future strategies to prevent or treat this malignancy. ..
  57. CYTOSKELETAL STRUCTURE IN THE INTESTINAL BRUSH BORDER
    Mark Mooseker; Fiscal Year: 1999
    ..To this end we propose the molecular cloning of the proteins which form the cytoskeleton of the Drosophila midgut BB--beginning with the MV core protein villin. ..
  58. SMC/MUC4-ErbB2 Complex: Implications on Neoplasia
    VICTORIA RAMSAUER; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  59. DEVELOPMENT OF GASTROINTESTINAL TRANSPORT MODELS
    Mary Moyer; Fiscal Year: 2001
    ..proposed commercial application: not available ..
  60. Functional Role of CaT1 and Calbindin D in Ca Transport
    Richard Wood; Fiscal Year: 2005
    ....
  61. NHE3 Regulation by Lipid Rafts and Hsc70
    Xuhang Li; Fiscal Year: 2004
    ..The proposed studies will lead to the understanding of two novel mechanisms by which epithelial NHE3 is regulated: compartmentation in lipid rafts and complex formation with Hsc70. ..
  62. 15-lipoxygenase products in colorectal differentiation
    Aaron Jacobs; Fiscal Year: 2005
    ..In addition, it will evaluate the role of 15-Lox and PPARs in promoting growth arrest in colorectal cancer, providing novel insight into the mechanisms of cancer biology. ..
  63. REGULATION OF UROGUANYLIN BY DIETARY ZINC STATUS
    Robert Cousins; Fiscal Year: 2000
    ..6) The researchers proposed to begin studies with T-84 cells or Caco-2 cells to test the impact of media zinc concentration on uroguanylin precursor mRNA and/or peptide levels. ..