hybrid cells

Summary

Top Publications

  1. ncbi Characterization of hybrid cells derived from spontaneous fusion events between breast epithelial cells exhibiting stem-like characteristics and breast cancer cells
    Thomas Dittmar
    Institute of Immunology, Zentrum für Biomedizinische Ausbildung und Forschung an der UWH ZBAF, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
    Clin Exp Metastasis 28:75-90. 2011
  2. ncbi Terahertz radiation induces spindle disturbances in human-hamster hybrid cells
    H Hintzsche
    Institut fur Pharmakologie und Toxikologie, Universitat Wurzburg, Germany
    Radiat Res 175:569-74. 2011
  3. pmc Spontaneous formation of tumorigenic hybrids between breast cancer and multipotent stromal cells is a source of tumor heterogeneity
    Germana Rappa
    Nevada Cancer Institute, Las Vegas, NV, USA
    Am J Pathol 180:2504-15. 2012
  4. ncbi Periodic activation of Wnt/beta-catenin signaling enhances somatic cell reprogramming mediated by cell fusion
    Frederic Lluis
    Telethon Institute of Genetics and Medicine, Via P Castellino, 111, 80131 Naples, Italy
    Cell Stem Cell 3:493-507. 2008
  5. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
  6. ncbi delta-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation
    Anika Heiss
    Institute of Pharmacology, Toxicology and Pharmacy Ludwig Maximilians University of Munich Koeniginstrasse 16, 80539 Muenchen, Federal Republic of Germany
    Exp Cell Res 315:2115-25. 2009
  7. pmc Gene expression pattern in transmitochondrial cytoplasmic hybrid cells harboring type 2 diabetes-associated mitochondrial DNA haplogroups
    Seungwoo Hwang
    Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    PLoS ONE 6:e22116. 2011
  8. ncbi Deciphering the molecular genetic basis of NPC through functional approaches
    Hong Lok Lung
    Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
    Semin Cancer Biol 22:87-95. 2012
  9. ncbi Cell fusion is a potent inducer of aneuploidy and drug resistance in tumor cell/ normal cell hybrids
    Benjamin Berndt
    Institute of Immunology, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
    Crit Rev Oncog 18:97-113. 2013
  10. pmc Horizontal transmission and retention of malignancy, as well as functional human genes, after spontaneous fusion of human glioblastoma and hamster host cells in vivo
    David M Goldenberg
    Garden State Cancer Center, Center for Molecular Medicine and Immunology, Morris Plains, NJ, USA
    Int J Cancer 131:49-58. 2012

Research Grants

  1. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
  2. Novel Molecular and Cellular Therapies in Fanconi Anemia
    David Williams; Fiscal Year: 2009
  3. RADIATION CYTOGENETICS
    Joel Bedford; Fiscal Year: 1993
  4. MOLECULAR GENETIC ANALYSIS OF XQ28
    Stephen Warren; Fiscal Year: 1992
  5. CHARACTERIZATION & REGULATION OF BETA-ENDORPHIN RECEPTOR
    Nancy Lee; Fiscal Year: 1992
  6. MACROPHAGE/NEUTROPHIL ELASTASE INHIBITOR
    EILEEN REMOLD O DONNELL; Fiscal Year: 1999
  7. GENETIC STUDIES OF HUMAN CELLS
    BARBARA MIGEON; Fiscal Year: 2001
  8. BETA GALACTOSIDASES IN GLOBOID CELL LEUKODYSTROPHY
    Thaddeus Kurczynski; Fiscal Year: 1980
  9. CHARACTERIZATION AND REGULATION OF BETA-ENDORPHIN RECEPT
    Nancy Lee; Fiscal Year: 2001
  10. GENETIC DISSECTION OF OCULAR CONNECTIVE TISSUE
    Robert Church; Fiscal Year: 1990

Detail Information

Publications207 found, 100 shown here

  1. ncbi Characterization of hybrid cells derived from spontaneous fusion events between breast epithelial cells exhibiting stem-like characteristics and breast cancer cells
    Thomas Dittmar
    Institute of Immunology, Zentrum für Biomedizinische Ausbildung und Forschung an der UWH ZBAF, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
    Clin Exp Metastasis 28:75-90. 2011
    ..In the present study we characterized hybrid cells derived from spontaneous fusion events between the breast epithelial cell line M13SV1-EGFP-Neo and two breast ..
  2. ncbi Terahertz radiation induces spindle disturbances in human-hamster hybrid cells
    H Hintzsche
    Institut fur Pharmakologie und Toxikologie, Universitat Wurzburg, Germany
    Radiat Res 175:569-74. 2011
    ..The findings in the present study do not necessarily imply disease or injury but may be important for evaluating possible underlying mechanisms...
  3. pmc Spontaneous formation of tumorigenic hybrids between breast cancer and multipotent stromal cells is a source of tumor heterogeneity
    Germana Rappa
    Nevada Cancer Institute, Las Vegas, NV, USA
    Am J Pathol 180:2504-15. 2012
    ..Our findings reconcile the fusion theory of cancer progression with the common observation that breast cancer metastases are generally aneuploid, but not tetraploid, and are histopathologically similar to the primary neoplasm...
  4. ncbi Periodic activation of Wnt/beta-catenin signaling enhances somatic cell reprogramming mediated by cell fusion
    Frederic Lluis
    Telethon Institute of Genetics and Medicine, Via P Castellino, 111, 80131 Naples, Italy
    Cell Stem Cell 3:493-507. 2008
    ..Our data thus demonstrate that in ESCs, periodic beta-catenin accumulation via the Wnt/beta-catenin pathway provides a specific threshold that leads to the reprogramming of somatic cells after fusion...
  5. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
    ....
  6. ncbi delta-Opioid receptor-stimulated Akt signaling in neuroblastoma x glioma (NG108-15) hybrid cells involves receptor tyrosine kinase-mediated PI3K activation
    Anika Heiss
    Institute of Pharmacology, Toxicology and Pharmacy Ludwig Maximilians University of Munich Koeniginstrasse 16, 80539 Muenchen, Federal Republic of Germany
    Exp Cell Res 315:2115-25. 2009
    ..the signal transduction pathway by which opioids induce Akt activation in neuroblastomaxglioma (NG108-15) hybrid cells. Exposure of the cells to both [D-Pen(2,5)]enkephalin and etorphine resulted in a time- and dose-dependent ..
  7. pmc Gene expression pattern in transmitochondrial cytoplasmic hybrid cells harboring type 2 diabetes-associated mitochondrial DNA haplogroups
    Seungwoo Hwang
    Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    PLoS ONE 6:e22116. 2011
    ....
  8. ncbi Deciphering the molecular genetic basis of NPC through functional approaches
    Hong Lok Lung
    Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, Pokfulam, Hong Kong SAR, People s Republic of China
    Semin Cancer Biol 22:87-95. 2012
    ..These studies show the advantages of using functional approaches for identification of TSGs...
  9. ncbi Cell fusion is a potent inducer of aneuploidy and drug resistance in tumor cell/ normal cell hybrids
    Benjamin Berndt
    Institute of Immunology, Witten Herdecke University, Stockumer Str 10, 58448 Witten, Germany
    Crit Rev Oncog 18:97-113. 2013
    ..Particularly in the tumor context, cell fusion has been associated with a progression of this disease since hybrid cells derived from fusion events between tumor cells and normal cells, such as macrophages and adult stem cells, ..
  10. pmc Horizontal transmission and retention of malignancy, as well as functional human genes, after spontaneous fusion of human glioblastoma and hamster host cells in vivo
    David M Goldenberg
    Garden State Cancer Center, Center for Molecular Medicine and Immunology, Morris Plains, NJ, USA
    Int J Cancer 131:49-58. 2012
    ....
  11. ncbi Changing potency by spontaneous fusion
    Qi Long Ying
    Centre for Genome Research, University of Edinburgh, The King s Buildings, West Mains Road, Edinburgh EH9 3JQ, UK
    Nature 416:545-8. 2002
    ..not arise by direct conversion of brain to embryonic stem cell but rather through spontaneous generation of hybrid cells. The tetraploid hybrids exhibit full pluripotent character, including multilineage contribution to chimaeras...
  12. ncbi Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells
    Chad A Cowan
    Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Science 309:1369-73. 2005
    ..Human embryonic stem (hES) cells were fused with human fibroblasts, resulting in hybrid cells that maintain a stable tetraploid DNA content and have morphology, growth rate, and antigen expression patterns ..
  13. ncbi Cell fusion is the principal source of bone-marrow-derived hepatocytes
    Xin Wang
    Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon 97239, USA
    Nature 422:897-901. 2003
    ..We conclude that hepatocytes derived form bone marrow arise from cell fusion and not by differentiation of haematopoietic stem cells...
  14. ncbi Pluripotential reprogramming of the somatic genome in hybrid cells occurs with the first cell cycle
    Dong Wook Han
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, 48149 Munster, Germany
    Stem Cells 26:445-54. 2008
    ....
  15. pmc mtDNA G10398A variant in African-American women with breast cancer provides resistance to apoptosis and promotes metastasis in mice
    Mariola Kulawiec
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    J Hum Genet 54:647-54. 2009
    ..Together our studies suggest that the G10398A variant confers resistance to apoptosis and promotes metastasis...
  16. ncbi Human monocyte x mouse melanoma fusion hybrids express human gene
    A K Chakraborty
    Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520, USA
    Gene 275:103-6. 2001
    ..This observation may lead us to find contributory genes from monocyte and/or macrophage that are responsible for modulating the genotypes and hence the phenotypes in the hybrids...
  17. ncbi Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells
    M Tada
    Precursory Research for Embryonic Science and Technology (PRESTO, JST, Kawaguchi-shi, Japan
    Curr Biol 11:1553-8. 2001
    ..The pluripotency of the ES-thymocyte hybrid cells is shown in vivo, since they contribute to all three primary germ layers of chimeric embryos...
  18. pmc Cybrid models of Parkinson's disease show variable mitochondrial biogenesis and genotype-respiration relationships
    Paula M Keeney
    Morris K Udall Parkinson s Research Center of Excellence, University of Virginia, PO Box 800394, Charlottesville, VA 22908, USA
    Exp Neurol 220:374-82. 2009
    ..Cybrids remain a valuable model for some aspects of sPD but their heterogeneity mitigates against a simple designation of sPD phenotype in this cell model...
  19. ncbi Erasure of cellular memory by fusion with pluripotent cells
    Jeong Tae Do
    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, Germany
    Stem Cells 25:1013-20. 2007
    ..F9-NSC hybrid cells displayed various features of reprogramming, such as reactivation of pluripotency genes, inactivation of tissue-..
  20. ncbi Pancreatic ductal cells acquire mesenchymal characteristics through cell fusion with bone marrow-derived mesenchymal stem cells and SIRT1 attenuates the apoptosis of hybrid cells
    Shanmiao Gou
    Pancreatic Disease Institute, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
    Cells Tissues Organs 196:129-36. 2012
    ..Cell fusion between somatic cells and bMSCs to form hybrid cells may have an important role in tissue repair through the subsequent reprogramming of the somatic cell nucleus...
  21. ncbi CD34 hybrid cells promote endothelial colony-forming cell bioactivity and therapeutic potential for ischemic diseases
    Jun Hee Lee
    Laboratory for Vascular Medicine and Stem Cell Biology, Medical Research Institute, Department of Physiology, School of Medicine, Pusan National University, Yangsan, Korea
    Arterioscler Thromb Vasc Biol 33:1622-34. 2013
    ..Although endothelial progenitor cells (EPCs) have been reported to promote neovessel formation during vascular injury, the function of supporting cells of EPCs and their interaction with EPCs during EPC isolation remain unclear...
  22. ncbi Monochromosome transfer provides functional evidence for growth-suppressive genes on chromosome 14 in nasopharyngeal carcinoma
    Yue Cheng
    Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong Special Administrative Region, China
    Genes Chromosomes Cancer 37:359-68. 2003
    ....
  23. ncbi Analysis of aberrant methylation of the VHL gene by transgenes, monochromosome transfer, and cell fusion
    I Kuzmin
    Intramural Research Support Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland, MD 21702, USA
    Oncogene 18:5672-9. 1999
    ..Contribution of some putative trans-acting factors cannot be excluded during a period when the aberrant VHL methylation pattern was first generated...
  24. ncbi Spontaneous hybridization of macrophages and Meth A sarcoma cells
    Lill Tove R Busund
    Department of Clinical Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
    Int J Cancer 98:573-81. 2002
    ..The macrophage DNA index was set at 1; Meth A cells were found to have an index of 1.6 in G1 phase, and the hybrids had a 2.6 index. The hybrids grew more slowly in vitro than Meth A cells, but grew faster in vivo...
  25. ncbi Oligonucleotide microarray expression analysis of genes whose expression is correlated with tumorigenic and non-tumorigenic phenotype of HeLa x human fibroblast hybrid cells
    S Nishizuka
    Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697 4025, USA
    Cancer Lett 165:201-9. 2001
    ..These findings suggest that synthetic oligonucleotide arrays are a powerful and highly reproducible tool to identify those genes whose expression is associated with certain phenotypes...
  26. ncbi Positive contribution of pathogenic mutations in the mitochondrial genome to the promotion of cancer by prevention from apoptosis
    Yujiro Shidara
    Department of Pathology, Tokyo Women s Medical University, School of Medicine, Tokyo, Japan
    Cancer Res 65:1655-63. 2005
    ..Because apoptosis occurred less frequently in the mutant versus wild-type cybrids in cultures and tumors, the pathogenic mtDNA mutations seem to promote tumors by preventing apoptosis...
  27. ncbi Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion
    Takashi Hayashi
    Center for Surgery Research, Cleveland Clinic Foundation, Ohio 44195, USA
    Clin Immunol 104:14-20. 2002
    ..These results provide an impetus for treating cancer patients with similarly generated cells...
  28. pmc Nucleosomes are translationally positioned on the active allele and rotationally positioned on the inactive allele of the HPRT promoter
    C Chen
    Department of Biochemistry and Molecular Biology, Center for Mammalian Genetics, University of Florida, Gainesville, 32610, USA
    Mol Cell Biol 21:7682-95. 2001
    ....
  29. ncbi Spontaneously formed tumorigenic hybrids of Meth A sarcoma and macrophages grow faster and are better vascularized than the parental tumor
    Lill Tove R Busund
    Department of Clinical Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway
    Int J Cancer 100:407-13. 2002
    ....
  30. ncbi Targeted chromosome elimination from ES-somatic hybrid cells
    Hiroyuki Matsumura
    Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Nat Methods 4:23-5. 2007
    ..we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single ..
  31. ncbi Purified hybrid cells from dendritic cell and tumor cell fusions are superior activators of antitumor immunity
    J Li
    Oncology Research Institute, Greenville Hospital System, SC 29605, USA
    Cancer Immunol Immunother 50:456-62. 2001
    ..Because of the difficulty of purifying the hybrid cells from the fusion, fusion mixtures were used in these studies...
  32. ncbi Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes
    Barbara A Boggs
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 30:73-6. 2002
    ....
  33. ncbi Transplanted bone marrow regenerates liver by cell fusion
    George Vassilopoulos
    Division of Hematology, University of Washington, Seattle, Washington 98195, USA
    Nature 422:901-4. 2003
    ..We also show that the haematopoietic donor genome adopts a more hepatocyte-specific expression profile after cell fusion, as the wild-type Fah gene was activated and the pan-haematopoietic CD45 marker was no longer expressed...
  34. pmc Mechanisms of the genotoxicity of crocidolite asbestos in mammalian cells: implication from mutation patterns induced by reactive oxygen species
    An Xu
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Environ Health Perspect 110:1003-8. 2002
    ..These results provide direct evidence that the mutagenicity of asbestos is mediated by ROS in mammalian cells...
  35. ncbi Tumour-dendritic hybrid cell vaccination for the treatment of patients with malignant melanoma: immunological effects and clinical results
    Uwe Trefzer
    Department of Dermatology and Allergy, Charite, University Medicine Berlin, Humboldt University, Schumannstr 20 21, 10117 Berlin, Germany
    Vaccine 23:2367-73. 2005
    ..Eleven of fourteen patients, clinical responders and non-responders alike, mounted high-frequency T cell responses to various tumour-associated antigens. Failing clinical responses correlated with loss of antigenicity...
  36. pmc Mutagenicity of arsenic in mammalian cells: role of reactive oxygen species
    T K Hei
    Center for Radiological Research, College of Physicians and Surgeons, School of Public Health, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 95:8103-7. 1998
    ..Thus, the carcinogenicity of arsenite can be explained at least in part by it being a mutagen that depends on reactive oxygen species for its activity...
  37. ncbi Enhancement of sodium current in NG108-15 cells during neural differentiation is mainly due to an increase in NaV1.7 expression
    Akinori Kawaguchi
    Department of Biological Chemistry, School of Pharmacy, Kinki University, Kowakae 3 4 1, Higasiosaka 577 8502, Japan
    Neurochem Res 32:1469-75. 2007
    ..These results suggest that the enhancement of TTX-sensitive sodium current density in differentiated NG108-15 cells is mainly due to the increase in the expression of the TTX-sensitive voltage-gated Na+ channel, NaV1.7...
  38. ncbi Mitochondrial respiration and respiration-associated proteins in cell lines created through Parkinson's subject mitochondrial transfer
    A Raquel Esteves
    Centro de Neurociencias e Biologia Celular, Universidade de Coimbra, Coimbra, Portugal
    J Neurochem 113:674-82. 2010
    ..We conclude mitochondrial respiration and pathways influenced by aerobic metabolism are altered in NT2 cybrid cell lines generated through transfer of PD subject platelet mitochondria...
  39. ncbi Phenotypic characteristics of hybrid cells produced by cell fusion of porcine adrenal chromaffin cells with human mesenchymal stem cells: a preliminary study
    Guangbin Shi
    The Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, 1601 West Taylor Street, Chicago, IL 60612, USA
    Neurol Res 30:217-22. 2008
    ....
  40. ncbi Hybrid cells formed by fusion of Epstein-Barr virus-associated B-lymphoblastoid cells and either marrow-derived or solid tumour-derived cell lines display different co-stimulatory phenotypes and abilities to activate allogeneic T-cell responses in vitro
    A L Cywinski
    Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK
    Tissue Antigens 68:115-26. 2006
    ..By contrast, hybrid cells derived from three solid tumours only expressed MHC class II when the parent tumour cell line expressed MHC ..
  41. pmc Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T
    Taku Amo
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 0XY, UK
    Gene 411:69-76. 2008
    ..We found that there were no functionally-important bioenergetic differences between mitochondria bearing these haplogroups, using either isolated mitochondria or mitochondria within cells...
  42. pmc The cybrid model of sporadic Parkinson's disease
    Patricia A Trimmer
    Morris K Udall Parkinson s Disease Research Center of Excellence, Department of Neurology, University of Virginia, Charlottesville, 22908, USA
    Exp Neurol 218:320-5. 2009
    ..sPD cybrid models can be improved by the use of non-tumor human stem cell-derived neural precursor cells and by an introduction of postmortem brain mtDNA to test its causality directly...
  43. pmc Senescence of immortal human fibroblasts by the introduction of normal human chromosome 6
    A K Sandhu
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey New Jersey Medical School, Newark 07103
    Proc Natl Acad Sci U S A 91:5498-502. 1994
    ..Based upon these results we propose that at least one of the genes (SEN6) for cellular senescence in human fibroblasts is present on the long arm of chromosome 6...
  44. pmc Were inefficient mitochondrial haplogroups selected during migrations of modern humans? A test using modular kinetic analysis of coupling in mitochondria from cybrid cell lines
    Taku Amo
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 404:345-51. 2007
    ..Contrary to the predictions of this hypothesis, mitochondria from Arctic haplogroups had similar or even greater coupling efficiency than mitochondria from tropical haplogroups...
  45. ncbi Mitochondrial function in Parkinson's disease cybrids containing an nt2 neuron-like nuclear background
    A Raquel F Esteves
    Centro de Neurociencias e Biologia Celular, Universidade de Coimbra, Hospitais da Universidade de Coimbra, 3004 Coimbra, Portugal
    Mitochondrion 8:219-28. 2008
    ..Equivalent MPP+ exposures are more likely to trigger programmed cell death in PD cybrid cells than in control cybrid cells. Our data support a relatively upstream role for mitochondrial dysfunction in idiopathic PD...
  46. ncbi Complementation of V(D)J recombination defect and X-ray sensitivity of scid mouse cells by human chromosome 8
    S S Banga
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103 2757
    Mutat Res 315:239-47. 1994
    ..These observations demonstrate that the human homolog of the mouse scid gene resides on human chromosome 8...
  47. pmc Hybrids of dendritic cells and tumor cells generated by electrofusion simultaneously present immunodominant epitopes from multiple human tumor-associated antigens in the context of MHC class I and class II molecules
    Maria R Parkhurst
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 170:5317-25. 2003
    b>Hybrid cells generated by fusing dendritic cells with tumor cells (DC-TC) are currently being evaluated as cancer vaccines in preclinical models and human immunization trials...
  48. pmc Relationships among molecular genetic and respiratory properties of Parkinson's disease cybrid cells show similarities to Parkinson's brain tissues
    M Kathleen Borland
    Center for the Study of Neurodegenerative Diseases, University of Virginia, Charlottesville, Virginia, USA
    Biochim Biophys Acta 1792:68-74. 2009
    ..This will require characterization of the abnormalities and dynamics of mtDNA changes propagated through sPD cybrids over time...
  49. ncbi Effective treatment of spontaneous metastases derived from a poorly immunogenic murine mammary carcinoma by combined dendritic-tumor hybrid vaccination and adoptive transfer of sensitized T cells
    Hidemasa Tamai
    Center for Surgery Research NE62, Department of Immunology, Cleveland Clinic, Cleveland, 9500 Euclid Ave, Cleveland, OH 44195, USA
    Clin Immunol 127:66-77. 2008
    ..Taken together, the combined approach may be useful for clinical development of cancer immunotherapy...
  50. ncbi Identification of highly expressed genes in metastasis-suppressed chromosome 6/human malignant melanoma hybrid cells using subtractive hybridization and differential display
    J H Lee
    The Jake Gittlen Cancer Research Institute, Pennsylvania State University College of Medicine, Hershey 17033 0850, USA
    Int J Cancer 71:1035-44. 1997
    ..9, following transfection and constitutive expression. Our results illustrate the power of subtractive hybridization and differential display to identify functional metastasis-controlling genes in human melanoma...
  51. ncbi The effectiveness of 20 mev protons at nanosecond pulse lengths in producing chromosome aberrations in human-hamster hybrid cells
    T E Schmid
    Klinikum rechts der Isar, Department of Radiation Oncology, Technische Universitat Munchen, D 81675 Munchen, Germany
    Radiat Res 175:719-27. 2011
    ....
  52. ncbi Generation of highly pure fusions of colorectal carcinoma and antigen-presenting cells
    Ulrike Klier
    Division of Molecular Oncology and Immunotherapy, Department of General Surgery, University of Rostock, Schillingallee 35, 18055, Rostock, Germany
    Langenbecks Arch Surg 395:365-71. 2010
    ..One approach is the fusion of antigen-presenting cells (APCs) with tumor cells. Hybrid cells combine the antigenicity of tumors with the immunostimulatory capacity of APCs...
  53. pmc Coexistence of alternative lengthening of telomeres and telomerase in hTERT-transfected GM847 cells
    K Perrem
    Cancer Research Group, Children's Medical Research Institute, Westmead, Sydney, New South Wales 2145, Australia
    Mol Cell Biol 21:3862-75. 2001
    ..These hybrid cells were telomerase positive, and the telomeres decreased in length, very rapidly at first and then at the rate ..
  54. ncbi Vaccination of dendritic cells loaded with interleukin-12-secreting cancer cells augments in vivo antitumor immunity: characteristics of syngeneic and allogeneic antigen-presenting cell cancer hybrid cells
    Takuji Suzuki
    Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging, and Cancer, Tohoku University, Sendai, Japan
    Clin Cancer Res 11:58-66. 2005
    ..This novel IL-12-producing fusion cell vaccine might be one promising intervention for future cancer immunotherapy...
  55. pmc A chemical enucleation method for the transfer of mitochondrial DNA to rho(o) cells
    Maria Pilar Bayona-Bafaluy
    Department of Neurology, University of Miami School of Medicine, Miami, FL 33136, USA
    Nucleic Acids Res 31:e98. 2003
    ..This method greatly facilitates the production of transmitochondrial cybrids, thereby increasing the number of mtDNA mutations and the recipient cell types that can be studied by this system...
  56. pmc A higher resolution radiation hybrid map of bovine chromosome 13
    Jörg Schläpfer
    Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, Bremgartenstrasse 109 A, 3012 Berne, Switzerland
    Genet Sel Evol 34:255-67. 2002
    ..The present map increases the marker density and the marker resolution on BTA13 and enables further insight into the evolutionary development of the chromosome as compared to man...
  57. ncbi Correction of a nucleotide-excision-repair mutation by human chromosome 19 in hamster-human hybrid cells
    L H Thompson
    Somat Cell Mol Genet 11:87-92. 1985
    ..This study demonstrates the mapping of a human repair gene by direct selection of complementing hybrids in the presence of a DNA-damaging agent (mitomycin C)...
  58. ncbi Semiallogeneic cancer vaccines formulated with granulocyte-macrophage colony-stimulating factor for patients with metastatic gastrointestinal adenocarcinomas: a pilot phase I study
    D A Newton
    Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, USA
    J Immunother 24:19-26. 2001
    ..Because of the simplicity, feasibility, and flexibility of this immunotherapeutic approach, semiallogeneic hybrid vaccines have the potential to be used in the treatment of virtually any type of cancer...
  59. ncbi The human X-inactivation centre is not required for maintenance of X-chromosome inactivation
    C J Brown
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106
    Nature 368:154-6. 1994
    ..Genes subject to X inactivation remain transcriptionally silent despite the loss of the XIC, demonstrating that the presence of the XIC is not required for the maintenance of X inactivation in somatic cells...
  60. ncbi Improvements in the use of neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins
    Elisabeth Cañete
    IRTA, Ctra Poble Nou, km 5 5, 43540 Sant Carles de la Rapita, Tarragona, Spain
    Toxicon 55:381-9. 2010
    ..Evaporation time of toxin solutions before cell exposure could be an important source of variability in AZA-1 toxic response evaluation...
  61. pmc Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning
    V Larionov
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Proc Natl Acad Sci U S A 93:13925-30. 1996
    ..Although < 2% of the DNA in the hybrid cells was human, as much as 80% of transformants had human DNA YACs when a TAR cloning vector contained Alu repeats...
  62. ncbi Variability of X chromosome inactivation: effect on levels of TIMP1 RNA and role of DNA methylation
    Catherine L Anderson
    Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    Hum Genet 110:271-8. 2002
    ..However, as women age, they may accumulate cells stably expressing TIMP1 from the inactive X, with a resulting increase of TIMP1, which may explain some sex differences in various late-onset disorders...
  63. ncbi Myeloma cell-induced disruption of bone remodelling compartments leads to osteolytic lesions and generation of osteoclast-myeloma hybrid cells
    Thomas L Andersen
    Department of Clinical Cell Biology, Vejle Hospital, CSFU IRS, University of Southern Denmark
    Br J Haematol 148:551-61. 2010
    ..increased bone resorption, and fusion of myeloma cells with osteoclasts thereby forming myeloma-osteoclast hybrid cells. These findings strongly support a critical role of BRC canopies in myeloma-induced bone disease...
  64. ncbi A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation
    M D'ESPOSITO
    International Institute of Genetics and Biophysics, CNR, Naples, Italy
    Nat Genet 13:227-9. 1996
    ..Unlike all Xp PAR genes studied so far, a synaptobrevin-like gene, tentatively named SYBL1, undergoes X inactivation. In addition, it is also inactive on the Y chromosome, thereby maintaining dosage compensation in an unprecedented way...
  65. ncbi Investigation of bystander effects in hybrid cells by means of cell fusion and premature chromosome condensation induction
    G I Terzoudi
    Institute of Radioisotopes and Radiodiagnostic Products, National Centre for Scientific Research Demokritos, Athens, Greece
    Radiat Res 173:789-801. 2010
    ..These observations and the methodology developed should be useful in the further development of protocols for investigating bystander responses and for elucidating the underlying mechanisms...
  66. ncbi Large scale mapping of methylcytosines in CTCF-binding sites in the human H19 promoter and aberrant hypomethylation in human bladder cancer
    D Takai
    Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA
    Hum Mol Genet 10:2619-26. 2001
    ..Loss of methylation imprinting of H19 is linked to hypomethylation of the paternal allele in human bladder cancer, unlike the situation in Wilms' tumor and colon cancer where the maternal allele becomes hypermethylated...
  67. ncbi Characteristics of hybrid cells obtained by dendritic cell/tumour cell fusion in a T-47D breast cancer cell line model indicate their potential as anti-tumour vaccines
    Karine Serhal
    EA3499, Universite Paris 6, Laboratoire d Histologie Biologie Tumorale, Hopital Tenon, AP HP, Paris, France
    Int J Oncol 31:1357-65. 2007
    ..identified using confocal microscopy to avoid an overestimation of fusion efficiency and to distinguish clearly hybrid cells from aggregated or phagocytosed cells...
  68. pmc The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells
    S F Dowdy
    Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine 92717
    Nucleic Acids Res 19:5763-9. 1991
    ..The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype...
  69. ncbi Chromosomal autonomy of hMLH1 methylation in colon cancer
    Hui Li
    Department of Microbiology and Molecular Biology, Case Western Reserve University, Cleveland, Ohio, OH 44106, USA
    Oncogene 21:1443-9. 2002
    ..We posit that hMLH1 methylation is dependent on and maintained by cis chromosomal marks, whose nature remains to be elucidated...
  70. ncbi Calretinin and calbindin D-28k, but not parvalbumin protect against glutamate-induced delayed excitotoxicity in transfected N18-RE 105 neuroblastoma-retina hybrid cells
    C D'Orlando
    Institute of Histology and General Embryology, University of Fribourg, CH 1705 Fribourg, Switzerland
    Brain Res 945:181-90. 2002
    ..We have stably transfected N18-RE 105 neuroblastoma-retina hybrid cells with the cDNAs for the three CaBPs and investigated the effect of these proteins on the L-glutamate-induced, Ca(..
  71. ncbi Constitutional translocation t(1;17)(p36.31-p36.13;q11.2-q12.1) in a neuroblastoma patient. Establishment of somatic cell hybrids and identification of PND/A12M2 on chromosome 1 and NF1/SCYA7 on chromosome 17 as breakpoint flanking single copy markers
    G Laureys
    Department of Pediatric Oncology, University Hospital, Ghent, Belgium
    Oncogene 10:1087-93. 1995
    ..As a pivotal step towards breakpoint cloning, at present these flanking markers optimally delineate the breakpoint regions of both chromosomes 1 and 17 at the molecular level...
  72. pmc Mutagenic effects of a single and an exact number of alpha particles in mammalian cells
    T K Hei
    Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 94:3765-70. 1997
    ..These data provide direct evidence that a single a particle traversing a nucleus will have a high probability of resulting in a mutation and highlight the need for radiation protection at low doses...
  73. ncbi Spontaneous fusion of cells between species yields transdifferentiation and retroviral transfer in vivo
    Brenda M Ogle
    Transplantation Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    FASEB J 18:548-50. 2004
    ..These hybrid cells divide, express human and porcine proteins, and contribute to porcine nonhematopoietic tissues...
  74. pmc Stabilization and localization of Xist RNA are controlled by separate mechanisms and are not sufficient for X inactivation
    C M Clemson
    Department of Cell Biology, University of Massachusetts Medical Center, Worcester, Massachusetts 01655, USA
    J Cell Biol 142:13-23. 1998
    ..XIST RNA does not localize in reactivants or in naturally inactive human X chromosomes in mouse/ human hybrid cells. The XIST transcripts are fully stabilized despite their inability to localize, and hence XIST RNA localization ..
  75. pmc Histone code modifications on pluripotential nuclei of reprogrammed somatic cells
    Hironobu Kimura
    Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
    Mol Cell Biol 24:5710-20. 2004
    ..Here, we show that the reprogrammed somatic genome of ES hybrid cells becomes hyperacetylated at H3 and H4, while lysine 4 (K4) of H3 becomes globally hyper-di- and -tri-methylated...
  76. ncbi Human chromosome 17 comparative anchor loci are conserved on bovine chromosome 19
    Y P Yang
    Department of Veterinary Pathobiology, Texas A and M University, College Station 77843, USA
    Genomics 27:293-7. 1995
    ....
  77. ncbi Mitochondrial damage mediates genotoxicity of arsenic in mammalian cells
    Su Xian Liu
    Center for Radiological Research, Department of Neurology, Columbia University, 630 West 168th Street, New York, NY 10032, USA
    Cancer Res 65:3236-42. 2005
    ....
  78. ncbi Differential gene expression in tumorigenic and nontumorigenic HeLa x normal human fibroblast hybrid cells
    H Tsujimoto
    Department of Microbiology and Molecular Genetics, University of California Irvine College of Medicine, Irvine, California, USA
    Mol Carcinog 26:298-304. 1999
    ..in the control of tumorigenic expression, we performed differential display screening of nontumorigenic hybrid cells and tumorigenic segregants...
  79. ncbi Comparative study of the use of neuroblastoma cells (Neuro-2a) and neuroblastomaxglioma hybrid cells (NG108-15) for the toxic effect quantification of marine toxins
    Elisabeth Cañete
    Centre d Aqüicultura, IRTA, Ctra Poble Nou s n Km 5 5, 43540 Sant Carles de la Rapita, Tarragona, Spain
    Toxicon 52:541-50. 2008
    ..Both cell types were able to identify Na-channel acting toxins and allowed to quantify the effect of saxitoxin, brevetoxin-3, palytoxin, okadaic acid, dinophysistoxin-1 and pectenotoxin-2 under different experimental conditions...
  80. ncbi Characterization of the toxic mechanism triggered by Alzheimer's amyloid-beta peptides via p75 neurotrophin receptor in neuronal hybrid cells
    Emi Tsukamoto
    Departments of Pharmacology and Anatomy, Keio University School of Medicine, Shinjuku ku, Tokyo, Japan
    J Neurosci Res 73:627-36. 2003
    ..Here we report that Abeta causes cell death in neuronal hybrid cells transfected with p75NTR, but not in nontransfected cells, and that p75NTR(L401K) cannot mediate Abeta ..
  81. pmc RHO--radiation hybrid ordering
    A Ben-Dor
    Department of Computer Science, Technion, Haifa 32000, Israel
    Genome Res 10:365-78. 2000
    ..For three chromosomes, the results differ significantly (lod score >1.75), with chromosome 2 having the largest improvement (lod score 3.776)...
  82. ncbi Reprogramming in inter-species embryonal carcinoma-somatic cell hybrids induces expression of pluripotency and differentiation markers
    Marzena Flasza
    Intercytex Ltd, Manchester, United Kingdom
    Cloning Stem Cells 5:339-54. 2003
    ..Colonies of hybrid cells displayed heterogeneity in cellular morphology as well as in their pattern of human gene expression...
  83. ncbi Regeneration of somatic hybrids in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica
    Fengning Xiang
    School of Life Sciences, Shandong University, Jinan 250100, Shandong, China
    Genome 47:680-8. 2004
    ..These results indicate that the regeneration of hybrid plants involves not only the integration of S. italica nuclear and organellar DNA, but also the genome complementation of T1 and T2...
  84. ncbi LIT1, an imprinted antisense RNA in the human KvLQT1 locus identified by screening for differentially expressed transcripts using monochromosomal hybrids
    K Mitsuya
    Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Nishimachi, Yonago, Japan
    Hum Mol Genet 8:1209-17. 1999
    ..Thus, this positional approach using human monochromosomal hybrids could contribute to the efficient identification of imprinted loci in humans...
  85. ncbi X inactivation analysis and DNA methylation studies of the ubiquitin activating enzyme E1 and PCTAIRE-1 genes in human and mouse
    L Carrel
    Department of Genetics and Center for Human Genetics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
    Hum Mol Genet 5:391-401. 1996
    ..Methylation of CpG residues at restriction sites at the 5' end of both genes on the murine inactive X chromosome is consistent with both genes being subject to X inactivation in mouse, in contrast to their expression status in humans...
  86. pmc Direct coupling of opioid receptors to both stimulatory and inhibitory guanine nucleotide-binding proteins in F-11 neuroblastoma-sensory neuron hybrid cells
    R A Cruciani
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461
    Proc Natl Acad Sci U S A 90:3019-23. 1993
    ..to the inhibitory and "other" G proteins, gi and Go, in F-11 (neuroblastoma-dorsal root ganglion neuron) hybrid cells. Treatment of intact F-11 cells with cholera toxin decreased specific binding of the opioid agonist [D-Ala2,D-..
  87. ncbi Mutagenicity of diesel exhaust particles mediated by cell-particle interaction in mammalian cells
    Lingzhi Bao
    Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Hefei, PR China
    Toxicology 229:91-100. 2007
    ..05). These results provided direct evidence that DEPs was mutagenic in mammalian cells and that cell-particle interaction played an essential role in the process...
  88. pmc Chromosomal localization of a human band 3-like gene to region 7q35----7q36
    A P Palumbo
    Am J Hum Genet 39:307-16. 1986
    ..In situ hybridization analysis using the c-DNA for this nonerythroid Band 3 gene further localized the gene to region 7q35----7q36 of human metaphase chromosomes...
  89. ncbi Assignment of the aquaporin-8 water channel gene (AQP8) to human chromosome 16p12
    L Viggiano
    Istituto di Genetica, Universita degli Studi di Bari, Italy
    Cytogenet Cell Genet 84:208-10. 1999
  90. ncbi Reactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST
    I Yoshida
    Research Center for Molecular Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan
    Exp Cell Res 230:208-19. 1997
    ..The level of XIST expression in our hybrid cells was almost identical to that of parental CF150 cells...
  91. ncbi Extinction of albumin gene expression in a panel of human chromosome 2 microcell hybrids
    K M Cerosaletti
    Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Genomics 31:348-58. 1996
    ..These data define and map a genetic locus on human chromosome 2 that extinguishes albumin gene expression in trans, and they suggest that TSE2-mediated extinction is independent of HNF-1, -4, -3 alpha, and -3 beta expression...
  92. pmc Polymorphic X-chromosome inactivation of the human TIMP1 gene
    C L Anderson
    Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    Am J Hum Genet 65:699-708. 1999
    ..However, in other cells, no expression of TIMP1 was observed from the inactive X chromosome, suggesting that TIMP1 inactivation is polymorphic in human females...
  93. ncbi Generation of hybrid cell lines with endothelial potential from spontaneous fusion of adult bone marrow cells with embryonic fibroblast feeder
    Jianwen Que
    National University Medical Institute, National University of Singapore, Singapore
    In Vitro Cell Dev Biol Anim 40:143-9. 2004
    ....
  94. ncbi Human embryonic stem cells reprogram myeloid precursors following cell-cell fusion
    Junying Yu
    Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53706, USA
    Stem Cells 24:168-76. 2006
    ..When the hybrid cells were formed into embryoid bodies, upregulation of genes characteristic of the three germ layers and ..
  95. ncbi Vaccination therapy with tumor-dendritic cell hybrids: a promising therapeutic approach?
    Holger A Haenssle
    Georg August University of Goettingen, Department of Dermatology, von Siebold Str 3, D 37075 Goettingen, Germany
    Curr Opin Investig Drugs 6:1240-5. 2005
    ..As an alternative to loading DCs with tumor antigen preparations, hybrid cells generated by fusing tumor cells with DCs have successfully been evaluated in preclinical cancer models...
  96. ncbi Activation of antitumor cytotoxic T lymphocytes by fusion of patient-derived dendritic cells with autologous osteosarcoma
    Z Yu
    Center of Orthopedic Surgery Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University, Xi an 710038, Shaanxi, China
    Exp Oncol 27:273-8. 2005
    ..This study was designed to investigate the antitumor effects of tumor vaccine produced by electrofusion between human osteosarcoma cells and DCs...
  97. ncbi Fusion hybrid of dendritic cells and engineered tumor cells expressing interleukin-12 induces type 1 immune responses against tumor
    Meiqing Shi
    Research Unit, Saskatchewan Cancer Agency, Department of Oncology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Tumori 91:531-8. 2005
    ..In the present study, we investigated the antitumor immunity derived from vaccination of fusion hybrids between DCs and engineered J558/IL-12 myeloma cells secreting Th1 cytokine IL-12...
  98. ncbi Hybrid-primed lymphocytes and hybrid vaccination prevent tumor growth of lewis lung carcinoma in mice
    Rajkumar Savai
    Department of Hematology and Oncology, Medical Center University of Giessen, Giessen, Germany
    J Immunother 29:175-87. 2006
    ..b>Hybrid cells (HCs) were used to explore 3 potential tumor therapy strategies: protective immunization, vaccination, and ..
  99. ncbi [Establishment of dendritomas by fusion of human dendritic cells with human hepatocellular carcinoma cell line HLE cells]
    Xin Guan
    Department of Hepatobiliary Surgery, Peking University People s Hospital, Beijing 100044, China
    Zhonghua Zhong Liu Za Zhi 27:465-7. 2005
    ..To construct dendritomas by fusion of human dendritic cells with HLE cells, a human hepatocellular carcinoma cell line...
  100. pmc Changes in replication, nuclear location, and expression of the Igh locus after fusion of a pre-B cell line with a T cell line
    Jie Zhou
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Immunol 175:2317-20. 2005
    ..These data are consistent with the silencing of a pre-B cell-specific replication program in the fusion hybrid cells and independent regulation of the nuclear location of Igh loci.
  101. ncbi [Immunotherapeutic reactivity of dendritic cells loaded with a variety of antigen preparations]
    Keiji Shimizu
    Dept of Surgery, Division of Digestive Surgery, Kyoto Prefectural University of Medicine
    Gan To Kagaku Ryoho 31:1637-9. 2004
    ..In conclusion, DC-tumor fusion hybrids were the most effective vaccine to eradicate existing tumors. These data support the use of DC-tumor electrofusion cells for the treatment of human cancer...

Research Grants62

  1. Generation of Human iPS Cells via Non-integrating Vectors
    Wen Shu Wu; Fiscal Year: 2010
    ..low efficiency and the requirement for fresh human oocytes or formation of somatic/embryonic stem (ES) hybrid cells, thereby limiting their further clinical applications...
  2. Novel Molecular and Cellular Therapies in Fanconi Anemia
    David Williams; Fiscal Year: 2009
    ..The complementation is based upon correction of the chromosomal sensitivity to cross-linking agents in hybrid cells. Nine independent genes have been cloned and characterized within these 11 complementation groups (A-C, D1, D2 ..
  3. RADIATION CYTOGENETICS
    Joel Bedford; Fiscal Year: 1993
    ..In addition, studies would be undertaken on the complementation in hybrid cells of a defect in cells from patients with Ataxia-Telangiectasia; first, to see whether the defect resulting in ..
  4. MOLECULAR GENETIC ANALYSIS OF XQ28
    Stephen Warren; Fiscal Year: 1992
    ..this will be accomplished by inducing, in the hybrid cells, chromosome breakage at the fragile X site (Xq27...
  5. CHARACTERIZATION & REGULATION OF BETA-ENDORPHIN RECEPTOR
    Nancy Lee; Fiscal Year: 1992
    ..rat periaqueductal gray (PAG), which is enriched in mu opioid receptors, NG108- 15 neuroblastoma x glioma hybrid cells, which contain a homogeneous population of delta receptors, and guinea pig cerebellum, which is enriched in ..
  6. MACROPHAGE/NEUTROPHIL ELASTASE INHIBITOR
    EILEEN REMOLD O DONNELL; Fiscal Year: 1999
    ..Chromosomal localization will be determined by the use of DNA from human-rodent hybrid cells. The promoter region of the HEI gene will be sequenced and characterized for regulatory elements after ..
  7. GENETIC STUDIES OF HUMAN CELLS
    BARBARA MIGEON; Fiscal Year: 2001
    ..These chromosomes, isolated in hybrid cells or enriched by cell cloning, will be genotyped mainly using PCR analysis to identify breakpoint regions; ..
  8. BETA GALACTOSIDASES IN GLOBOID CELL LEUKODYSTROPHY
    Thaddeus Kurczynski; Fiscal Year: 1980
    ..Procedures for cell hybridization of cultured canine and human fibroblasts and selection of hybrid cells will be developed. Various genetic crosses will be made utilizing fibroblasts from humans and dogs with GLD...
  9. CHARACTERIZATION AND REGULATION OF BETA-ENDORPHIN RECEPT
    Nancy Lee; Fiscal Year: 2001
    ..rat periaqueductal gray (PAG), which is enriched in mu opioid receptors, NG108- 15 neuroblastoma x glioma hybrid cells, which contain a homogeneous population of delta receptors, and guinea pig cerebellum, which is enriched in ..
  10. GENETIC DISSECTION OF OCULAR CONNECTIVE TISSUE
    Robert Church; Fiscal Year: 1990
    ..or absence of the antigen or recombinant probe will determine whether the specific chromosome is present in the hybrid cells, thus localizing the gene to its chromosome...
  11. MONOCLONAL ANTIBODIES TO HUMAN EPIDERMIS
    Lowell Goldsmith; Fiscal Year: 1980
    ..Drug resistance and temperature will be used to select against the parental cell types and only hybrid cells will grow...
  12. IMMORTALIZATION OF SALIVARY GLAND EPITHELIAL CELL LINES
    JANICE MORAND; Fiscal Year: 1993
    ..and morphological characteristics of the immortalized cells will be analyzed to determine the extent to which hybrid cells retain salivary gland-specific properties...
  13. ULTRAVIOLET AND IONIZING RADIATION DAMAGE
    HENRY GRIGGS; Fiscal Year: 1990
    ..which possess efficient photorepair, mammalian cells lacking efficient photorepair, and mammalian-nonmammalian hybrid cells are to be treated with combinations of gamma radiation, ultraviolet light, photo-reactivating light, and ..
  14. NAD METABOLISM AND CHROMOSOME LOSS IN HYBRID CELLS
    Martin Rechsteiner; Fiscal Year: 1980
    ..in the attempt to determine the mechanism underlying human chromosome loss from newly-formed human mouse hybrid cells. Among the various measurements we will make are the kinetics of human chromosome loss, the number and ..
  15. Functional Cloning of Hutchinson-Gliford progeria gene
    Junko Oshima; Fiscal Year: 2004
    ..autosomal dominant mutation, HGPS and normal fibroblasts will be fused, and the gene expression profile of the hybrid cells will be analyzed by microarrays...
  16. High Precision Optical One-to-One Cell Fusion
    Gao Chen; Fiscal Year: 2009
    ..that combines the technologies of picodroplet and optical fusion to rapidly generate hundreds or thousands of hybrid cells with very high precision...
  17. EXPRESSION OF EPSTEIN-BARR VIRUS IN HYBRID CELLS
    Ronald Glaser; Fiscal Year: 1980
    ..EBV DNA synthesized early after infection showed a higher S value than linear 55S EBV DNA. At late stage, homogeneous unit length DNA was detected. ..
  18. CELLULAR ASPECTS OF GRANULOSA CELL DIFFERENTIATION
    Anthony Zeleznik; Fiscal Year: 1980
    ..b>Hybrid cells will be monitored for 1) their ability to bind radioiodinated FSH or HCG to determine if these cells possess ..
  19. MOLECULAR CHARACTERIZATION OF A HUMAN CENTROMERE
    Immo Scheffler; Fiscal Year: 1991
    The project has two distinct components: 1. A series of human/hamster hybrid cells have been isolated which contain human DNA exclusively in the form of a minichromosome...
  20. RECESSIVE TUMOR GENES IN NEUROECTODERMAL MALIGNANCIES
    NICHOLAS DRACOPOLI; Fiscal Year: 1990
    ..Dominant selectable markers will be transfected into reduced mouse-human hybrid cells which contain either an intact chromosome 1 or segments of this chromosome...
  21. IDENTIFICATION OF HUMAN LYMPHOCYTE SURFACE ANTIGENS
    Ian Trowbridge; Fiscal Year: 1980
    ..monospecific antisera against the human cell surface component by immunization of larger animals with the hybrid cells. The significance of this work lies in the new approach used to study human lymphocyte antigens in which ..
  22. DEREGULATION OF ONCOGENE EXPRESSION IN HUMAN TUMORS
    SUSAN ASTRIN; Fiscal Year: 1993
    ..hybrids between cells expressing regulated c-myc and colon carcinoma cells expressing deregulated myc result in hybrid cells in which myc is regulated normally...
  23. Microfluidic-based high-efficiency cell fusion for studying nuclear reprogramming
    Joel Voldman; Fiscal Year: 2009
    ..and a special geometry to efficiently arrange cells side-by-side to create a large fraction of properly paired hybrid cells. We are developing this technology to study nuclear reprogramming, which is the process whereby a somatic cell&..
  24. Mechanism of nucleolar dominance in Arabidopsis
    Sasha Preuss; Fiscal Year: 2005
    ..Nucleolar dominance in hybrid cells is thought to be an extreme extension by which the non-hybrid cell may control the extant of ribosomal gene ..
  25. NEURODEGENERATIVE DISEASES AND NEURAL CELL TRANSPLANTS
    KEDAR PRASAD; Fiscal Year: 2000
    ..and then test their efficacy in improving neurological deficits of rats with 6-OHDA lesions; c) to establish hybrid cells by fusing DA-producing immortalized nerve cells with glia cells, characterize them, and then test their ..
  26. CELL GENETIC DAMAGE AT LOW DOSE AND LOW DOSE RATES
    CHARLES WALDREN; Fiscal Year: 2000
    ..g. high and low dose rates. Mutation analysis will be carried out in different constructs of AL human-hamster hybrid cells in which a spectrum of mutations from 100,000 to 1...
  27. ELECTROPERMEABILIZATION/FUSION OF LIPID VESICLES & CELLS
    David Needham; Fiscal Year: 1993
    ..Studies on parental myeloma and genetic hybrid cells (hybridomas) will also be made in order to extend this work from selectively reconstituted systems to cells of ..
  28. FUSION OF ANIMAL CELL CLONES--LIFE SPAN OF HYBRID CELLS
    James Smith; Fiscal Year: 1980
    ..Mutant human diploid fibroblasts will be used as one fusion parent to allow biochemical selection of the hybrid cells. For these experiments we will fuse: 1) two normal human diploid clones with varying proliferative potential; 2)..
  29. MAPPING MELANOMA METASTASIS SUPPRESSOR GENE(S)
    MARY MIELE; Fiscal Year: 2001
    ..3-q23 ( about40Mb) by similar studies using #6 fragment. Presently our laboratory has hybrid cells that contain a transferred #6 deleted at 6q21 ( about4Mb), a region frequently lost in many types of advanced ..
  30. SOMATIC CELL GENETICS OF AMINOACYL-TRNA SYNTHETASES
    Richard Denney; Fiscal Year: 1980
    ..b>Hybrid cells will be selected in HAT medium, and their secreted products tested for anti-aminoacyl-tRNA synthetase activity ..
  31. Regulation of BCL-2 and Tumorigenicity
    Marc Mendonca; Fiscal Year: 2006
    ..abstract_text> ..
  32. PATHOPHYSIOLOGY IN NEURODEGENERATION
    Russell Swerdlow; Fiscal Year: 2002
    ....
  33. MECHANISMS OF FIBER CARCINOGENESIS
    TOM HEI; Fiscal Year: 2001
    ..Cytogenetic and molecular alterations at the gene level will be analyzed from tumors and intermediate transformation stages to provide a mechanistic basis for tumor induction by mineral fibers. ..
  34. THE MUTAGENICITY OF MINERAL FIBERS
    TOM HEI; Fiscal Year: 2006
    ..By using specific DNA probes of other genes that have been regionally mapped to various sites on chromosome 11, the molecular spectrum of mutations induced by either asbestos or glass fiber control will be compared. ..
  35. MECHANISMS OF ARSENIC CARCINOGENESIS
    TOM HEI; Fiscal Year: 2005
    ..abstract_text> ..
  36. 11th & 12th Conference on Cancer Therapy with Antibodies and Immunoconjugates
    David Goldenberg; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  37. 9/10TH Conf./Cancer Therapy/Antibody/Immunoconjugates
    David Goldenberg; Fiscal Year: 2004
    ..The conference chairman, Dr. Goldenberg, has secured two outstanding scientists as co-chairmen, Drs. Ralph Reisfeld (Scripps) and Edward Sausville (NCI). ..
  38. EXTRACELLULAR MATRIX ABNORMALITIES IN CORNEAL EDEMA
    MARIA KENNEY; Fiscal Year: 2002
    ....
  39. Abnormalities in Keratoconus Corneas
    MARIA KENNEY; Fiscal Year: 2003
    ..These studies will provide fundamental insights into KC pathogensis and may provide a basis for therapy. ..
  40. CMMI Human Subject Research Enhancement Program
    David Goldenberg; Fiscal Year: 2002
    ..3.) Provide travel expenses and educational materials for IRB members, investigators, and Regulatory Affairs Office personnel to attend meetings for educational enhancement. ..
  41. PROSTATE CELL PROLIFERATION IN CANCER AND AGING
    ANA SOTO; Fiscal Year: 2005
    ..We expect that this research will lead to the development of markers to identify this phenotype, and to the development of specific therapeutic strategies. ..
  42. PROGRESSION OF CHRONIC MYELOGENOUS LEUKEMIA
    Scott Dessain; Fiscal Year: 2004
    ..The second two parts explore the role of oncogenes in the pathogenesis of blast phase CML and attempt to build a multi-step model to explain the progression of chronic phase CML to blast phase. ..
  43. Loss of FLJ13639 Expression in Acute Leukemia
    Keshav K Singh; Fiscal Year: 2010
    ..Confirmation of the role of the loss of FLJ13639 in leukemia prognosis, increase of chemo resistance should lead us and others, to develop new prognostic tools as well as novel, innovative therapeutic strategies. ..
  44. HIGH ENERGY IONS AND GENOMIC INSTABILITY
    Eric Hall; Fiscal Year: 2001
    ....
  45. Genetic studies of c-myc gene function in the cell cycle
    John Sedivy; Fiscal Year: 2006
    ..These studies are expected to shed light on how specific outputs, such as the promotion of cell growth or cell Cycle progression, are causally associated with specific cellular targets and molecular mechanisms. [unreadable] [unreadable]..
  46. Cytochrome Oxidase Polymorphisms in Aging
    Russell Swerdlow; Fiscal Year: 2007
    ..This work could help explain why complex genetic diseases are sometimes associated with mitochondrial dysfunction. ..
  47. GORDON CONFERENCE ON ENVIRONMENTAL ENDOCRINE DISRUPTORS
    ANA SOTO; Fiscal Year: 2002
    ..abstract_text> ..
  48. Mitochondria and Mutagenesis
    Keshav K Singh; Fiscal Year: 2010
    ..abstract_text> ..
  49. Mitochondria and Carcinogenesis
    Keshav K Singh; Fiscal Year: 2010
    ..The proposed study will help determine the contribution of mutant mtDNA to the development of human cancer. ..
  50. Developmental Regulation of MacroH2A1 Chromatin Assembly
    Theodore Rasmussen; Fiscal Year: 2009
    ..Research proposed here will provide basic insights into differentiation biology and provide a framework for future attempts to achieve rationally-guided ES cell differentiation. ..
  51. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2009
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  52. MITDNA MUTAGENESIS BY ENVIRONMENTAL CARCINOGENS
    Keshav Singh; Fiscal Year: 2003
    ..The proposed study will fill a major gap in our understanding of the mechanisms of mitDNA mutagenesis induced by environmental carcinogens which has direct relevance in on the induction of mitochondrial diseases. ..
  53. IMMUNOTHERAPY WITH ELECTROFUSED DENDRITIC TUMOR HYBRIDS
    Suyu Shu; Fiscal Year: 2004
    ..Interestingly, electrofusion of immature DCs with tumor cells resulted in hybrid cells having characteristics of mature DCs with high expression of MHC class II, B7.1 and B7.2 molecules...
  54. Genetic Analysis of Cell-Type-Specific Function
    RAYMOND FOURNIER; Fiscal Year: 2006
    ..The overall goal of these experiments will be a comprehensive definition and analysis of regulatory elements in individual chromatin domains. ..
  55. Role of the ABC Transporter ABCB6 in Cytotoxicity
    John Schuetz; Fiscal Year: 2006
    ..Cumulatively, these studies will lead to better understanding of this important mitochondrial ABC transporter in mitochondrial dysfunction and diseases such as cancer. ./" ..
  56. Astrocytes As Multipotent Stem Cells
    ERIC LAYWELL; Fiscal Year: 2003
    ....
  57. NURR1 EXPRESSION AND DAERGIC DYSFUNCTION RELEVANT TO PD
    Wei Dong LE; Fiscal Year: 2003
    ....
  58. Perinatal xenoestrogen exposure: epigenesis and neoplasia
    ANA SOTO; Fiscal Year: 2007
    ..This will also have a great impact on the way we study risk factors and conduct epidemiological studies; it may even influence public policy about breast cancer prevention. [unreadable] [unreadable] [unreadable] [unreadable]..
  59. Effectors of senescent states in human fibroblasts
    John Sedivy; Fiscal Year: 2008
    ..Studies described in this proposal will give us a better understanding of cellular senescence processes, and will begin to shed light on the roles of cellular senescence in organismal aging. [unreadable] [unreadable]..
  60. Functional Analysis of CDKp110 Protein Kinase
    Jill M Lahti; Fiscal Year: 2010
    ..What is the function of the CK11p110 isoform in the developing embryo? Is it possible to obtain viable embryos if only CDK11p110 or CDK11 is ablated? If so, are these mice more prone to tumors or developmental defects? ..