clone cells

Summary

Summary: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)

Top Publications

  1. ncbi Heterogeneity in cancer: cancer stem cells versus clonal evolution
    Mark Shackleton
    Howard Hughes Medical Institute, Life Sciences Institute, Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 138:822-9. 2009
  2. pmc Characterization of clonogenic multiple myeloma cells
    William Matsui
    Sidney Kimmel Comprehensive Cancer Center, John Hopkins University School of Medicine, Bunting Blaustein Cancer Research Bldg, Rm 245, 1650 Orleans St, Baltimore, MD 21231, USA
    Blood 103:2332-6. 2004
  3. ncbi Transcriptome-wide noise controls lineage choice in mammalian progenitor cells
    Hannah H Chang
    Vascular Biology Programme, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 453:544-7. 2008
  4. ncbi Mouse germ line stem cells undergo rapid and stochastic turnover
    Allon M Klein
    Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Stem Cell 7:214-24. 2010
  5. ncbi HIV-1 integration in the human genome favors active genes and local hotspots
    Astrid R W Schroder
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 110:521-9. 2002
  6. pmc Cancer regression and autoimmunity in patients after clonal repopulation with antitumor lymphocytes
    Mark E Dudley
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20902, USA
    Science 298:850-4. 2002
  7. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
  8. ncbi Control of compartment development by the engrailed gene in Drosophila
    G Morata
    Nature 255:614-7. 1975
  9. pmc The hematopoietic stem compartment consists of a limited number of discrete stem cell subsets
    Hans B Sieburg
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92112, USA
    Blood 107:2311-6. 2006
  10. ncbi Human T-cell memory consists mainly of unexpanded clones
    Paul L Klarenbeek
    Department of Clinical Immunology and Rheumatology, AMC University of Amsterdam, The Netherlands
    Immunol Lett 133:42-8. 2010

Research Grants

Detail Information

Publications302 found, 100 shown here

  1. ncbi Heterogeneity in cancer: cancer stem cells versus clonal evolution
    Mark Shackleton
    Howard Hughes Medical Institute, Life Sciences Institute, Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 138:822-9. 2009
    ....
  2. pmc Characterization of clonogenic multiple myeloma cells
    William Matsui
    Sidney Kimmel Comprehensive Cancer Center, John Hopkins University School of Medicine, Bunting Blaustein Cancer Research Bldg, Rm 245, 1650 Orleans St, Baltimore, MD 21231, USA
    Blood 103:2332-6. 2004
    ..These data suggest that MM "stem cells" are CD138- B cells with the ability to replicate and subsequently differentiate into malignant CD138+ plasma cells...
  3. ncbi Transcriptome-wide noise controls lineage choice in mammalian progenitor cells
    Hannah H Chang
    Vascular Biology Programme, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 453:544-7. 2008
    ....
  4. ncbi Mouse germ line stem cells undergo rapid and stochastic turnover
    Allon M Klein
    Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Stem Cell 7:214-24. 2010
    ..These results suggest that short-lived stem cells may be a common feature of mammalian stem cell systems and reveal a natural mechanism for matching the rates of cell proliferation and loss in tissue...
  5. ncbi HIV-1 integration in the human genome favors active genes and local hotspots
    Astrid R W Schroder
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 110:521-9. 2002
    ..4 kb region containing 1% of sites. These data document unexpectedly strong biases in integration site selection and suggest how selective targeting promotes aggressive HIV replication...
  6. pmc Cancer regression and autoimmunity in patients after clonal repopulation with antitumor lymphocytes
    Mark E Dudley
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20902, USA
    Science 298:850-4. 2002
    ..This approach presents new possibilities for the treatment of patients with cancer as well as patients with human immunodeficiency virus-related acquired immunodeficiency syndrome and other infectious diseases...
  7. ncbi Viable offspring derived from fetal and adult mammalian cells
    I Wilmut
    Roslin Institute Edinburgh, Roslin, Midlothian, UK
    Nature 385:810-3. 1997
    ....
  8. ncbi Control of compartment development by the engrailed gene in Drosophila
    G Morata
    Nature 255:614-7. 1975
  9. pmc The hematopoietic stem compartment consists of a limited number of discrete stem cell subsets
    Hans B Sieburg
    Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92112, USA
    Blood 107:2311-6. 2006
    ..These data from the steady-state HSC repertoire could provide a basis for the diagnosis of perturbed patterns of HSCs potentially caused by disease or aging...
  10. ncbi Human T-cell memory consists mainly of unexpanded clones
    Paul L Klarenbeek
    Department of Clinical Immunology and Rheumatology, AMC University of Amsterdam, The Netherlands
    Immunol Lett 133:42-8. 2010
    ..It raises new questions on the factors that steer maturation of memory phenotype and determine the size of memory clones...
  11. pmc Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses
    David A Price
    Human Immunology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 202:1349-61. 2005
    ..Vaccine strategies that reconstruct these biological processes could generate T cell populations that mediate optimal delivery of antiviral effector function...
  12. ncbi Cellular barcoding tool for clonal analysis in the hematopoietic system
    Alice Gerrits
    Department of Cell Biology, Section Stem Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Blood 115:2610-8. 2010
    ..This cellular barcoding tool permits a simple, sensitive assessment of clonality and holds great promise for future gene therapy protocols in humans, and any other applications when clonal tracking is important...
  13. ncbi Development of mesenchymal stem cells partially originate from the neural crest
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, 35 Shinano machi, Shinjuku ku, Tokyo 160 8582, Japan
    Biochem Biophys Res Commun 379:1114-9. 2009
    ..Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest...
  14. pmc Subclonal phylogenetic structures in cancer revealed by ultra-deep sequencing
    Peter J Campbell
    Wellcome Trust Sanger Institute, Hinxton CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:13081-6. 2008
    ..This study demonstrates the potential for ultra-deep resequencing to recapitulate the dynamics of clonal evolution in cancer cell populations...
  15. ncbi A single type of progenitor cell maintains normal epidermis
    Elizabeth Clayton
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Cambridge CB2 0XZ, UK
    Nature 446:185-9. 2007
    ..These cells are found to undergo both symmetric and asymmetric division at rates that ensure epidermal homeostasis. The results raise important questions about the potential role of stem cells on tissue maintenance in vivo...
  16. pmc Scribble mutants promote aPKC and JNK-dependent epithelial neoplasia independently of Crumbs
    Gregory R Leong
    Peter MacCallum Cancer Centre, Victoria, Australia
    BMC Biol 7:62. 2009
    ..Increasing evidence also implicates the loss of human Scrib in cancer. In this report, we investigate how loss of Scrib promotes epithelial tumourigenesis in Drosophila, both alone and in cooperation with oncogenic mutations...
  17. ncbi Clonal analysis of TET2 and JAK2 mutations suggests that TET2 can be a late event in the progression of myeloproliferative neoplasms
    Franz X Schaub
    Department of Biomedicine, Experimental Hematology, University Hospital Basel, Hebelstr 20, 4031 Basel, Switzerland
    Blood 115:2003-7. 2010
    ..The lack of a strict temporal order of occurrence makes it unlikely that mutations in TET2 represent a predisposing event for acquiring mutations in JAK2...
  18. ncbi Persistent survival of prevalent clonotypes within an immunodominant HIV gag-specific CD8+ T cell response
    David J van Bockel
    St Vincent s Centre for Applied Medical Research, University of New South Wales, Sydney, New South Wales, Australia
    J Immunol 186:359-71. 2011
    ....
  19. ncbi Establishment and maintenance of planar epithelial cell polarity by asymmetric cadherin bridges: a computer model
    Jean François Le Garrec
    Modélisation Dynamique des Systèmes Biologiques Intégrés, CNRS UMR 7138 Systématique, Adaptation, Evolution, Universite Pierre et Marie Curie, Paris, France
    Dev Dyn 235:235-46. 2006
    ..These features, largely independent of model details, may provide useful clues for future experimental efforts...
  20. pmc Selective growth, in vitro and in vivo, of individual T cell clones from tumor-infiltrating lymphocytes obtained from patients with melanoma
    Juhua Zhou
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 173:7622-9. 2004
    ..A similar analysis may also be useful for monitoring autoimmune responses...
  21. ncbi Energetic heavy ions accelerate differentiation in the descendants of irradiated normal human diploid fibroblasts
    Nobuyuki Hamada
    Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
    Mutat Res 637:190-6. 2008
    ..Accelerated differentiation may account, at least in part, for dose- and LET-dependent delayed loss of clonogenicity in normal human diploid cells, and could be a defensive mechanism that minimizes further expansion of aberrant cells...
  22. ncbi Identification of human oral keratinocyte stem/progenitor cells by neurotrophin receptor p75 and the role of neurotrophin/p75 signaling
    Takahiro Nakamura
    Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo ku, Kyoto 602 0841, Japan
    Stem Cells 25:628-38. 2007
    ..Our in vivo and in vitro findings indicate that p75 is a potential marker of oral keratinocyte stem/progenitor cells and that some neurotrophin/p75 signaling affects cell growth and survival...
  23. ncbi Inflamed target tissue provides a specific niche for highly expanded T-cell clones in early human autoimmune disease
    P L Klarenbeek
    Department of Clinical Immunology and Rheumatology, Academic Medical Center University of Amsterdam, Amsterdam, The Netherlands
    Ann Rheum Dis 71:1088-93. 2012
    ....
  24. ncbi The HLA A*0201-restricted hTERT(540-548) peptide is not detected on tumor cells by a CTL clone or a high-affinity T-cell receptor
    Marco A Purbhoo
    Avidex Ltd, 57 59 Milton Park, Abingdon, OX14 4RX, United Kingdom
    Mol Cancer Ther 6:2081-91. 2007
    ..We propose that, in future, rigorous methods must be applied for the validation of peptide epitopes used for clinical applications...
  25. pmc Distinct roles for long-term hematopoietic stem cells and erythroid precursor cells in a murine model of Jak2V617F-mediated polycythemia vera
    Ann Mullally
    Division of Hematology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Blood 120:166-72. 2012
    ..These findings recapitulate the development of post-PV myelofibrosis in human myeloproliferative neoplasms. In aggregate, these results demonstrate the distinct roles of LT-HSCs and erythroid precursors in the pathogenesis of PV...
  26. pmc Reconstruction of rat retinal progenitor cell lineages in vitro reveals a surprising degree of stochasticity in cell fate decisions
    Francisco L A F Gomes
    Cellular Neurobiology Research Unit, Institut de Recherches Cliniques de Montreal, Montreal, QC H2W 1R7, Canada
    Development 138:227-35. 2011
    ....
  27. ncbi Core-specific adaptive regulatory T-cells in different outcomes of hepatitis C
    Bettina Langhans
    Department of Internal Medicine I, University of Bonn, Sigmund Freud Strasse 25, 53105 Bonn, Germany
    Clin Sci (Lond) 119:97-109. 2010
    ..Thus HCV core-specific Treg-cells may contribute to the immunoregulatory balance in chronic hepatitis C...
  28. ncbi CMV-specific TCR-transgenic T cells for immunotherapy
    Andrea Schub
    Department of Gene Vectors, Helmholtz Zentrum Munchen, Munich, Germany
    J Immunol 183:6819-30. 2009
    ..Hence, we expect these CMV-TCR-transgenic T cells to be effective in controlling acute CMV disease and establishing an antiviral memory...
  29. ncbi Phenotypic and functional characterization of CD4 T cells expressing killer Ig-like receptors
    Jeroen van Bergen
    Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
    J Immunol 173:6719-26. 2004
    ..Together, our data indicate that KIR-expressing CD4 T cells are predominantly HLA class II-restricted effector memory Th1 cells, and that a significant, previously unrecognized fraction of effector memory Th1 cells expresses KIR...
  30. ncbi Impact of negative selection on the T cell repertoire reactive to a self-peptide: a large fraction of T cell clones escapes clonal deletion
    C Bouneaud
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris, France
    Immunity 13:829-40. 2000
    ..These results suggest that numerous low-affinity self-specific T cells persist in the periphery and show that a deleting ligand can specifically narrow the structural diversity of the TCR repertoire...
  31. pmc Identification of novel Ras-cooperating oncogenes in Drosophila melanogaster: a RhoGEF/Rho-family/JNK pathway is a central driver of tumorigenesis
    Anthony M Brumby
    Peter MacCallum Cancer Center, Melbourne, Victoria, Australia
    Genetics 188:105-25. 2011
    ..Collectively our analysis reveals the importance of the RhoGEF/Rho-family/JNK pathway in cooperative tumorigenesis with Ras(ACT)...
  32. pmc Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques
    Diane L Bolton
    Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 184:303-14. 2010
    ..The lack of impact following transfer of such a large number of functional Ag-specific CD8(+) T cells on SIV replication may reflect the magnitude of the immune response required to contain the virus...
  33. ncbi Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans
    R H Whitehead
    Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, Royal Melbourne Hospital, Melbourne, Australia
    Gastroenterology 117:858-65. 1999
    ..The factors controlling the proliferation and differentiation of the colonic mucosa are unknown and have proved difficult to identify mainly because of a lack of in vitro methods for studying the proliferative cells of the mucosa...
  34. ncbi Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes
    S L Mansour
    Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City 84112
    Nature 336:348-52. 1988
    ..The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene...
  35. pmc Multiple HLA class II-restricted melanocyte differentiation antigens are recognized by tumor-infiltrating lymphocytes from a patient with melanoma
    Paul F Robbins
    Surgery Branch, National Cancer Institute, National Institutes of Health, Building 10, Room 2B42, Bethesda, MD 20892 7502, USA
    J Immunol 169:6036-47. 2002
    ..These observations provide an impetus to develop strategies directed toward generating HLA class II-restricted tumor-reactive T cells...
  36. pmc A comprehensive survey of clonal diversity measures in Barrett's esophagus as biomarkers of progression to esophageal adenocarcinoma
    Lauren M F Merlo
    Molecular and Cellular Oncogenesis Program, The Wistar Institute, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania19104, USA
    Cancer Prev Res (Phila) 3:1388-97. 2010
    ..001). The type of alterations evaluated had little effect on the predictive value of most of the diversity measures. In summary, diversity measures are robust predictors of progression to cancer in this cohort...
  37. pmc Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
    Satoru Morikawa
    Department of Physiology, Keio University School of Medicine, Shinjuku ku, Tokyo 160 8582, Japan
    J Exp Med 206:2483-96. 2009
    ..Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities...
  38. pmc Generation of functional erythrocytes from human embryonic stem cell-derived definitive hematopoiesis
    Feng Ma
    Division of Cellular Therapy, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Proc Natl Acad Sci U S A 105:13087-92. 2008
    ..The present study should provide an experimental model for exploring early development of human erythropoiesis and hemoglobin switching and may help in the discovery of drugs for hereditary diseases in erythrocyte development...
  39. ncbi A unique three-dimensional model for evaluating the impact of therapy on multiple myeloma
    Julia Kirshner
    Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, AB, Canada
    Blood 112:2935-45. 2008
    ..The 3-D culture provides a novel biologically relevant preclinical model for evaluating therapeutic vulnerabilities of all compartments of the MM clone, including presumptive drug-resistant MM stem cells...
  40. ncbi Characterization of murine JAK2V617F-positive myeloproliferative disease
    Thomas G P Bumm
    Center for Hematologic Malignancies, Oregon Health and Science University Cancer Institute, Portland, OR 97239, USA
    Cancer Res 66:11156-65. 2006
    ..The disease was not transplantable and prolonged observation showed normalization of blood counts in most JAK2(V617F) mice, suggesting that the mutation may not confer self-renewal capacity...
  41. ncbi Extrusion of cells with inappropriate Dpp signaling from Drosophila wing disc epithelia
    Jie Shen
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Science 307:1789-90. 2005
    ..This loss correlates with extensive cytoskeletal changes followed by basal epithelial extrusion. We propose that Dpp promotes cell survival within disc epithelia by affecting cytoskeletal organization...
  42. ncbi Type 1 T regulatory cells
    M G Roncarolo
    San Raffaele Telethon Institute of Gene Therapy HSR TIGET, San Raffaele Scientific Institute, Milan, Italy
    Immunol Rev 182:68-79. 2001
    ..The use of Tr1 cells to identify novel targets for the development of new therapeutic agents, and as a cellular therapy to modulate peripheral tolerance, can be foreseen...
  43. ncbi Coordination of growth and cell division in the Drosophila wing
    T P Neufeld
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cell 93:1183-93. 1998
    ..We infer that dE2F and RBF function specifically in cell cycle control, and that cell cycle acceleration is insufficient to stimulate growth. Variations in dE2F activity could be used to coordinate cell division with growth...
  44. ncbi Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity
    Suzanne Bixby
    Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan, 1500 E Medical Center Drive, Ann Arbor, MI 48109, USA
    Neuron 35:643-56. 2002
    ..Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined...
  45. ncbi Leukemic B cells clonally identical to myeloma plasma cells are myelomagenic in NOD/SCID mice
    Linda M Pilarski
    Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, Canada
    Exp Hematol 30:221-8. 2002
    ....
  46. pmc An episomal mammalian replicon: sequence-independent binding of the origin recognition complex
    Daniel Schaarschmidt
    Department of Biology, Universitat Konstanz, Konstanz, Germany
    EMBO J 23:191-201. 2004
    ..It indicates that specific DNA sequences are not required for the chromatin binding of ORC in vivo. The conclusion is that epigenetic mechanisms determine the sites where mammalian DNA replication is initiated...
  47. ncbi Multiple BCR-ABL kinase domain mutations confer polyclonal resistance to the tyrosine kinase inhibitor imatinib (STI571) in chronic phase and blast crisis chronic myeloid leukemia
    Neil P Shah
    Department of Medicine, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Cancer Cell 2:117-25. 2002
    ..Multiple independent mutant clones were detected in a subset of relapsed cases. Our data support a clonal selection model of preexisting BCR-ABL mutations that confer imatinib resistance...
  48. ncbi Propagation, expansion, and multilineage differentiation of human somatic stem cells from dermal progenitors
    Georg Bartsch
    Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Stem Cells Dev 14:337-48. 2005
    ..Clonal growth of MSCs presents profound implications in our understanding of differentiation and development, and should provide a valuable resource for tissue repair...
  49. ncbi In vivo clonal analyses reveal the properties of endogenous neural stem cell proliferation in the adult mammalian forebrain
    C M Morshead
    Neurobiology Research Group, Department of Anatomy and Cell Biology, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    Development 125:2251-61. 1998
    ..There were calculated to be 1200-1300 cells between the rostral corpus callosum and rostral anterior commissure; these data support a lineage model similar to those based on stem cell behavior in other tissue types...
  50. pmc Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells
    Guokai Chen
    Morgridge Institute for Research, Madison, WI 53707 7365, USA
    Cell Stem Cell 7:240-8. 2010
    ..ROCK inhibition decreases phosphorylation of myosin light chain, suggesting that inhibition of actin-myosin contraction is also the mechanism through which ROCK inhibitors increase cloning efficiency of human ESCs...
  51. ncbi Generation of pluripotent stem cells from adult mouse liver and stomach cells
    Takashi Aoi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 321:699-702. 2008
    ..These data suggest that iPS cells are generated by direct reprogramming of lineage-committed somatic cells and that retroviral integration into specific sites is not required...
  52. ncbi Dangerous small B-cell clones
    Giampaolo Merlini
    Amyloid Center, Biotechnology Research Laboratories, Foundation IRCCS Policlinico San Matteo, Piazzale Golgi 2, 27100 Pavia, Italy
    Blood 108:2520-30. 2006
    ..Although the treatment of these conditions is not optimal, significant advances have been made, improving the duration and quality of life...
  53. ncbi The clonal origin of myocardial cells in different regions of the embryonic mouse heart
    Sigolène M Meilhac
    CNRS URA 2578, Departement de Biologie du Developpement, Institut Pasteur, 25 28 Rue du Dr Roux, 75724 Paris Cedex 15, France
    Dev Cell 6:685-98. 2004
    ..These results are not consistent with the prespecification of the cardiac tube as a segmented structure. They are discussed in the context of different heart fields and of the evolution of the heart...
  54. ncbi The clonal evolution of metastases from primary serous epithelial ovarian cancers
    Lalarukh Khalique
    Gynaecological Cancer Research Laboratories, UCL EGA Institute for Women s Health, University College London, London, UK
    Int J Cancer 124:1579-86. 2009
    ....
  55. ncbi Alkaline phosphatase-positive colony formation is a sensitive, specific, and quantitative indicator of undifferentiated human embryonic stem cells
    Michael D O'Connor
    Terry Fox Laboratory, 675 West 10th Avenue, Vancouver, British Columbia, Canada V5Z 1L3
    Stem Cells 26:1109-16. 2008
    ..Its use under standardized assay conditions should enhance future elucidation of the mechanisms that regulate hESC propagation and their early differentiation...
  56. ncbi Genetic diversity and geographical dispersal in grapevine clones revealed by microsatellite markers
    Ximena Moncada
    Laboratorio de Biotecnologia, Centro de Investigación La Platina, Instituto de Investigaciones Agropecuarias, INIA, Chile, PO Box 439 3 Santiago, Chile
    Genome 49:1459-72. 2006
    ..The possibility that this kind of genetic variability occurs in other vegetatively propagated species is discussed, focusing on possible fingerprinting applications...
  57. pmc Timing neurogenesis and differentiation: insights from quantitative clonal analyses of cerebellar granule cells
    J Sebastian Espinosa
    Howard Hughes Medical Institute and Department of Biology, Neurosciences Program, Stanford University, Stanford, California 94305, USA
    J Neurosci 28:2301-12. 2008
    ..These properties produce GC clones that are distinct, each having a restricted axonal projection, but that are on average similar in cell number. We discuss possible developmental mechanisms and functional implications of these findings...
  58. pmc Recurrence of autoreactive antigen-specific CD4+ T cells in autoimmune diabetes after pancreas transplantation
    Elsa Laughlin
    Benaroya Research Institute at Virginia Mason, 1201 Ninth Avenue, Seattle, WA 98101, USA
    Clin Immunol 128:23-30. 2008
    ..Comparisons of TCR clonotypes by cDNA sequencing revealed that identical T cells were present in the circulation, separated by long time intervals, consistent with a persistent memory response associated with recurrent autoimmunity...
  59. ncbi Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow)
    Antonio P Beltrami
    Centro Interdipartimentale Medicina Rigenerativa, University of Udine, Piazzale Santa Maria della Misericordia, 33100 Udine, Italy
    Blood 110:3438-46. 2007
    ....
  60. pmc The narrowing of the CD8 T cell repertoire in old age
    Marcia A Blackman
    Trudeau Institute, 154 Algonquin Ave, Saranac Lake, NY 12983, USA
    Curr Opin Immunol 23:537-42. 2011
    ....
  61. ncbi Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity
    Tomoyuki Yamanaka
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Japan
    Curr Biol 13:734-43. 2003
    ..However, how physically separated proteins interact remains to be clarified...
  62. ncbi Mitochondrial defect drives non-autonomous tumour progression through Hippo signalling in Drosophila
    Shizue Ohsawa
    Division of Genetics, Kobe University Graduate School of Medicine, 7 5 1 Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
    Nature 490:547-51. 2012
    ..Our findings provide a mechanistic basis for interclonal tumour progression driven by mitochondrial dysfunction and oncogenic Ras...
  63. ncbi Mutation of JAK2 in the myeloproliferative disorders: timing, clonality studies, cytogenetic associations, and role in leukemic transformation
    Peter J Campbell
    Department of Haematology, University of Cambridge, Cambridge, United Kingdom
    Blood 108:3548-55. 2006
    ..In 3 patients the leukemic cells were V617F(-), suggesting that in these patients the leukemia arose in a V617F(-) cell...
  64. pmc WT1 mutations in T-ALL
    Valeria Tosello
    Institute for Cancer Genetics, Columbia University, New York, NY 10032, USA
    Blood 114:1038-45. 2009
    ..Survival analysis demonstrated that WT1 mutations do not confer adverse prognosis in pediatric and adult T-ALL. Overall, these results identify the presence of WT1 mutations as a recurrent genetic alteration in T-ALL...
  65. ncbi Deciphering the mammary epithelial cell hierarchy
    John Stingl
    Department of Pathology, University of Cambridge, Cambridge, UK
    Cell Cycle 5:1519-22. 2006
    ..Suggestive parallels between mouse and human mammary cells point to the likelihood that a similarly structured multi-step differentiation program characterizes the mammary gland from both species...
  66. ncbi Two compensatory pathways maintain long-term stability and diversity in CD8 T cell memory repertoires
    Elena N Naumova
    Department of Public Health and Family Medicine, Tufts University School of Medicine, Boston, MA 02111, USA
    J Immunol 183:2851-8. 2009
    ..The recruitment of new clonotypes into the low-frequency component of the repertoire implies a role for these clonotypes...
  67. pmc Single cell analysis reveals similar functional competence of dominant and nondominant CD8 T-cell clonotypes
    Daniel E Speiser
    Ludwig Center for Cancer Research, University of Lausanne, Lausanne CH 1011, Switzerland
    Proc Natl Acad Sci U S A 108:15318-23. 2011
    ..In parallel, this was also found for EBV- and CMV-specific T cells. Thus, the nondominant clonotypes may contribute similarly to immunity as their dominant counterparts...
  68. ncbi RGB marking facilitates multicolor clonal cell tracking
    Kristoffer Weber
    Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Cancer Center Hamburg, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Nat Med 17:504-9. 2011
    ..We conclude that RGB marking represents a useful tool for cell clonality studies in tissue regeneration and pathology...
  69. pmc Ultra-sensitive detection of rare T cell clones
    Harlan Robins
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, United States
    J Immunol Methods 375:14-9. 2012
    ..The precision of the assay is demonstrated across five orders of magnitude. The accuracy for each clone is within an overall factor of three across the 100,000 fold dynamic range. Additionally, the assay is shown to be highly repeatable...
  70. ncbi Heterogeneity of anticancer drug sensitivity in squamous cell carcinoma of the tongue
    Minako Suzuki
    Oral and Maxillofacial Surgery and Systemic Medicine, Graduate School of Life Dentistry at Niigata, The Nippon Dental University, Niigata, Japan
    Hum Cell 24:21-9. 2011
    ..After examining anticancer drug sensitivity at the single cell level, we believe that anticancer drug-resistant genes may be involved in the heterogeneity of anticancer drug sensitivity with respect to cancer cell groups...
  71. ncbi Tob is a negative regulator of activation that is expressed in anergic and quiescent T cells
    D Tzachanis
    Department of Adult Oncology, Dana Farber Cancer Institute, Division of Medical Oncology, Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 2:1174-82. 2001
    ..Thus, T cell quiescence is an actively maintained phenotype that must be suppressed for T cell activation to occur...
  72. ncbi Comparative clonal analysis of reconstitution kinetics after transplantation of hematopoietic stem cells gene marked with a lentiviral SIN or a γ-retroviral LTR vector
    Kerstin Cornils
    Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Medical Centre Hamburg Eppendorf, Hamburg, Germany
    Exp Hematol 41:28-38.e3. 2013
    ..Finally, our results confirm the notion that marking with lentiviral self-inactivating vectors is associated with a lower risk of genotoxicity as compared with γ-retroviral LTR vectors...
  73. ncbi Counting stem cells: methodological constraints
    Leonid V Bystrykh
    Laboratory of Stem Cell Research, European Research Institute for the Biology of Ageing, University Medical Center Groningen, University of Groningen, The Netherlands
    Nat Methods 9:567-74. 2012
    ..We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies...
  74. ncbi Epstein-Barr nuclear antigen 1-specific CD4(+) Th1 cells kill Burkitt's lymphoma cells
    Casper Paludan
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10021, USA
    J Immunol 169:1593-603. 2002
    ..Therefore, adaptive immunity can be directed against Burkitt's lymphoma, and perhaps this role for CD4(+) Th1 cells extends to other tumors that escape MHC class I presentation...
  75. pmc HIV-specific CD8+ T cells from HIV+ individuals receiving HAART can be expanded ex vivo to augment systemic and mucosal immunity in vivo
    Aude G Chapuis
    Program in Immunology, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, WA 98109, USA
    Blood 117:5391-402. 2011
    ....
  76. ncbi How to improve the success rate of mouse cloning technology
    Nguyen Van Thuan
    Department of Animal Bioscience and Biotechnology, Konkuk University, South Korea
    J Reprod Dev 56:20-30. 2010
    ..This review describes how to improve the efficiency of cloning, the establishment of clone-derived embryonic stem cells and further applications...
  77. ncbi Chromosomal alterations cause the high rates and wide ranges of drug resistance in cancer cells
    Ruhong Li
    Department of Molecular and Cell Biology, Donner Laboratory, University of California, Berkeley, Berkeley, CA 94720, USA
    Cancer Genet Cytogenet 163:44-56. 2005
    ..The degree of resistance was roughly proportional to the number of these aneusomies. Thus, aneuploidy is the primary cause of the high rates and wide ranges of drug resistance in cancer cells...
  78. ncbi Alpha 3 domain mutants of peptide/MHC class I multimers allow the selective isolation of high avidity tumor-reactive CD8 T cells
    Mikael J Pittet
    Division of Clinical Onco Immunology, Ludwig Institute for Cancer Research, University Hospital, Lausanne, Switzerland
    J Immunol 171:1844-9. 2003
    ..Our results are relevant for ex vivo identification and isolation of T cells with potent antitumor activity for adoptive T cell therapy...
  79. ncbi Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells
    Paola Bonfanti
    Laboratory of Stem Cell Dynamics, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1015 Lausanne, Switzerland
    Nature 466:978-82. 2010
    ..Hence, microenvironmental cues are sufficient here to re-direct epithelial cell fate, allowing crossing of primitive germ layer boundaries and an increase in potency...
  80. pmc Persistence of multiple tumor-specific T-cell clones is associated with complete tumor regression in a melanoma patient receiving adoptive cell transfer therapy
    Juhua Zhou
    Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunother 28:53-62. 2005
    ....
  81. ncbi Methicillin-resistant coagulase-negative staphylococci in the community: high homology of SCCmec IVa between Staphylococcus epidermidis and major clones of methicillin-resistant Staphylococcus aureus
    Francois Barbier
    Bacteriology Unit and National Reference Center for Emergence of Resistance in Commensal Flora, 2EA 3964, Bichat Claude Bernard Hospital, Assistance Publique Hôpitaux de Paris and Xavier Bichat Medical School, Denis Diderot Paris 7 University, Paris, France
    J Infect Dis 202:270-81. 2010
    ..We assessed their potential role as a reservoir of staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)...
  82. pmc Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization
    Sylvain Brisse
    Institut Pasteur, Genotyping of Pathogens and Public Health, Paris, France
    PLoS ONE 4:e4982. 2009
    ..pneumoniae...
  83. ncbi On the karyotypic origin and evolution of cancer cells
    Joshua M Nicholson
    Department of Molecular and Cell Biology, Donner Laboratory, University of California Berkeley, Berkeley, CA 94720, USA
    Cancer Genet Cytogenet 194:96-110. 2009
    ..In sum, genomes consisting of individual and flexible karyotypes explain the characteristic individuality, stability, and flexibility of cancers...
  84. ncbi Clonal variation in phenotype and life span of human embryonic fibroblasts (MRC-5) transduced with the catalytic component of telomerase (hTERT)
    S Franco
    Laboratory of Developmental Hematopoiesis, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Exp Cell Res 268:14-25. 2001
    ..In addition, fibroblast-specific biological parameters, such as colony size, production of collagenase, and response to MMC and gamma radiation were tightly regulated at the clonal and subclonal levels...
  85. ncbi Human natural killer cells with polyclonal lectin and immunoglobulinlike receptors develop from single hematopoietic stem cells with preferential expression of NKG2A and KIR2DL2/L3/S2
    J S Miller
    Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota Cancer Center, Harvard Street at East River Road, Minneapolis, MN 55455, USA
    Blood 98:705-13. 2001
    ..Blood. 2001;98:705-713)..
  86. ncbi Chromosomal aberrations are shared by malignant plasma cells and a small fraction of circulating CD19+ cells in patients with myeloma and monoclonal gammopathy of undetermined significance
    Niklas Zojer
    First Department of Internal Medicine and Medical Oncology, Wilhelminenspital, University of Vienna, Montleartstrasse 37, 1160 Vienna, Austria
    Br J Haematol 117:852-9. 2002
    ..These results provide evidence that the circulating clonotypic B cells are closely related to the malignant plasma cells in myeloma and MGUS...
  87. ncbi The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells
    K Ito
    Yamamoto Behaviour Genes Project, ERATO Exploratory Research for Advanced Technology, JST Japan Science and Technology Corporation, at Mitsubishi Kasei Institute of Life Sciences, Minami Ooya, Tokyo, Japan
    Development 124:761-71. 1997
    ..Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures...
  88. ncbi Insight into the origin and clonal history of B-cell tumors as revealed by analysis of immunoglobulin variable region genes
    F Stevenson
    Tenovus Laboratory, Southampton University Hospitals Trust, UK
    Immunol Rev 162:247-59. 1998
    ..It also has a clear clinical relevance in tracking tumor cells, measuring residual disease, and finally in offering the opportunity of developing vaccines for treatment...
  89. ncbi Acute myeloid leukemia originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity
    Kristin J Hope
    Division of Cell and Molecular Biology, University Health Network, and Department of Molecular Genetics and Microbiology, University of Toronto, 620 University Avenue, Toronto, Ontario, M5G 2C1, Canada
    Nat Immunol 5:738-43. 2004
    ..Furthermore, normal developmental processes are not completely abolished during leukemogenesis. The existence of multiple stem cell classes shows the need for LSC-targeted therapies...
  90. ncbi Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936
    J J M van Dongen
    Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands
    Leukemia 17:2257-317. 2003
    ..The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease...
  91. ncbi Invariant NKT cells biased for IL-5 production act as crucial regulators of inflammation
    Kaori Sakuishi
    Department of Immunology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan
    J Immunol 179:3452-62. 2007
    ..The iNKT cell subset producing IL-5 and IL-13 could play a major role in the development of allergic disease or asthma and also in the immune regulation of Th1 inflammation...
  92. ncbi Reproducible but dynamic positioning of DNA in chromosomes during mitosis
    S Dietzel
    Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, 61801, USA
    Nat Cell Biol 3:767-70. 2001
    ..Unfolding intermediates during anaphase and telophase suggest that sequence repositioning occurs through the global uncoiling of an underlying chromatid structure...
  93. ncbi Self-renewal and solid tumor stem cells
    Muhammad Al-Hajj
    University of Michigan Medical School, CCGC Room 4410, 1500 E Medical Center Drive, Ann Arbor 48109 0936, USA
    Oncogene 23:7274-82. 2004
    ..This suggests that agents that target the defective self-renewal pathways in cancer cells might lead to improved outcomes in the treatment of these diseases...
  94. ncbi Memory inflation: continuous accumulation of antiviral CD8+ T cells over time
    Urs Karrer
    Nuffield Department of Clinical Medicine, University of Oxford, Peter Medawar Building for Pathogen Research, South Parks Road, Oxford OX1 3SY, UK
    J Immunol 170:2022-9. 2003
    ..This may be relevant for understanding the evolution of the large human CMV-specific CD8+ T cell populations seen in humans...
  95. ncbi Identification of cancer stem cell-like side population cells in human nasopharyngeal carcinoma cell line
    Jing Wang
    State Key Laboratory of Oncology in Southern China and Department of Experimental Research, Sun Yat sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510 060, P R China
    Cancer Res 67:3716-24. 2007
    ..Taken together, our data shed light on tumorigenesis and therapeutic-resistant mechanisms, which are helpful for developing novel targets for effective clinical treatment of NPC...
  96. ncbi High-avidity CTL exploit two complementary mechanisms to provide better protection against viral infection than low-avidity CTL
    M Derby
    Molecular Immunogenetics and Vaccine Research Section, Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1578, USA
    J Immunol 166:1690-7. 2001
    ....
  97. pmc Analysis of the copy number profiles of several tumor samples from the same patient reveals the successive steps in tumorigenesis
    Eric Letouze
    INSERM, UMR S 973, MTI, Universite Paris Diderot Paris 7, 35 rue Helene Brion, 75205 Paris Cedex 13, France
    Genome Biol 11:R76. 2010
    ....
  98. pmc Population genetics of cancer cell clones: possible implications of cancer stem cells
    Christopher T Naugler
    Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, C414, Diagnostic and Scientific Centre, 9, 3535 Research Road NW, Calgary AB, T2L 2K8 Canada
    Theor Biol Med Model 7:42. 2010
    ..Cancer cell clones can be conceptualized as sympatric asexual species, and as such, the application of theoretical population genetics as it pertains to asexual species may provide additional insights...
  99. ncbi One NY-ESO-1-derived epitope that promiscuously binds to multiple HLA-DR and HLA-DP4 molecules and stimulates autologous CD4+ T cells from patients with NY-ESO-1-expressing melanoma
    Maja Mandic
    Department of Medicine and The Melanoma Center, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    J Immunol 174:1751-9. 2005
    ..They also support the relevance of cancer vaccine trials with peptides NY-ESO-1 87-111 in the large number of cancer patients with NY-ESO-1-expressing tumors...
  100. ncbi Different NK cell surface phenotypes defined by the DX9 antibody are due to KIR3DL1 gene polymorphism
    C M Gardiner
    Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 166:2992-3001. 2001
    ....
  101. ncbi A rhoptry-protein-associated mechanism of clonal phenotypic variation in rodent malaria
    P R Preiser
    Division of Parasitology, National Institute for Medical Research, London, UK
    Nature 398:618-22. 1999
    ..This phenomenon is genetically and functionally distinct from classical antigenic variation, which is mediated by the var multigene family of P. falciparum...

Research Grants69

  1. Prediction of Lupus Outcome by Gene Expression Patterns
    Robert Winchester; Fiscal Year: 2006
    ..Second, whether CellCept will prove to be efficacious in a different gene expression subset, suggesting it could be used in these cases as a less toxic alternative to Cytoxan. ..
  2. Immunopathogenic features in calcific aortic stenosis
    Robert Winchester; Fiscal Year: 2009
    ..abstract_text> ..
  3. Measurement & modulation of the mutation rate in humans
    David Araten; Fiscal Year: 2007
    ..These studies will begin to elucidate the relationship between f and cancer risk and provide a highly relevant in vitro parameter with which to evaluate chemoprevention. ..
  4. RHESUS MACAQUE MODEL OF TUBERCULOSIS
    DAVID LEWINSOHN; Fiscal Year: 2001
    ..g., CT scans, quantitative culture of mycobacteria from BAL fluids and liver biopsies) which correlate with survival. ..
  5. GENERATION OF THOMSEN-FREIDENREICH-SPECIFIC T CELLS
    Alessandra Franco; Fiscal Year: 2002
    ..Two strategies will be compared: 1) passive immunotherapy by T cell transfer with selected T cell clones; and 2) active vaccination with the glycopeptides and glycolipids to generate a TF-specific T cell response. ..
  6. HSV-SERONEGATIVE PERSONS WITH AQUIRED IMMUNITY TO HSV
    Christine Posavad; Fiscal Year: 2002
    ..abstract_text> ..
  7. Oxygen Guided Hypofractionated Radiotherapy for Gliomas
    Nadeem Khan; Fiscal Year: 2010
    ....
  8. Large-scale chromatin structure and dynamics
    Andrew Belmont; Fiscal Year: 2007
    ..Knowledge gained from this research should provide valuable insight into regulation of basic molecular processes and may serve to guide design of future gene therapy vectors and stable transgene expression. ..
  9. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  10. Dev & Testing of New Medications for Treatment of Emerging Infectious Diseases
    Mitchell Avery; Fiscal Year: 2006
    ..This allows LADDS infrastructure to inch forward now to the last stages of drug development that are achievable with academic economics. [unreadable] [unreadable] [unreadable] [unreadable] [unreadable] [unreadable]..
  11. Becton Dickinson LSR-II Analytical Cytometer (BD-LSR-II)
    David Serreze; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  12. GFP-Based Assays to Probe Transcriptional Controls(RMI)
    Richard Katz; Fiscal Year: 2005
    ..The assays have the potential to reveal new lead compounds, as well as new gene targets for therapeutics. ..
  13. Autoimmune Oophoritis: Consequences of Gamete Vaccines
    Kenneth Tung; Fiscal Year: 2006
    ..Specifically, we will determine the immunogenetic regulation of the immune response to CP2, and the influence of the B cell epitope specificity of the ZP Ab on the induction of progenic AOD. ..
  14. Proteomics Analysis of T Cell Autoantigens in TID2
    Kathryn Haskins; Fiscal Year: 2005
    ..abstract_text> ..
  15. A novel set of molecular markers to measure metastatic neuroblastoma
    Nai Kong Cheung; Fiscal Year: 2007
    ..Armed with the tools to collect samples expeditiously and to measure MRD accurately, the paradigm of treating subclinical NB can then be tested in multicenter randomized studies. [unreadable] [unreadable]..
  16. THE MECHANISMS OF AUTOIMMUNE RESPONSE INITIATION IN MULTIPLE SCLEROSIS
    Silva Markovic Plese; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  17. Role of Redox Dependent Signaling in Leukemia
    Joya Chandra; Fiscal Year: 2010
    ..Tactics that quelch ROS production, or block downstream signaling through Fyn in BCR/ABL containing cells may provide promising therapeutic modalities. ..
  18. Lactate Transport as a Therapeutic Target in Glioma
    SAROJ PRIYANTHA MATHUPALA; Fiscal Year: 2010
    ..Thus, we need new ways to target this deadly cancer. We plan to test a novel method to "pickle-the-tumor-to-death," while leaving healthy brain cells intact. ..
  19. LANGERHANS CELLS AND NERVES: BIDIRECTIONAL SIGNALING
    Richard Granstein; Fiscal Year: 2009
    ..Immune responses in the skin. ..
  20. Comparative genetics of Lesch-Nyhan disease
    James Thomas; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  21. 2008 Musculoskeletal Biology and Bioengineering Gordon Research Conference
    Farshid Guilak; Fiscal Year: 2008
    ..The meeting will consist of 9 separate sessions, consisting of invited speakers and selected attendees at various levels of career development. [unreadable] [unreadable] [unreadable] [unreadable]..
  22. Chlamydia-infected Epithelial Cell Secretion of Immunoregulatory Cytokines
    WILBERT DERBIGNY; Fiscal Year: 2008
    ..unreadable] DESCRIPTION (provided by applicant): NO ABSTRACT WAS PROVIDED BY THE PI. [unreadable] [unreadable] [unreadable] [unreadable]..
  23. Epigenetic control of hematopoietic stem cells
    Christa Muller Sieburg; Fiscal Year: 2007
    ..Relevance: Data from the proposed studies may provide better ways to treat leukopenia and may help in the design of approaches to selectively eradicate leukemic stem cells. ..
  24. T CELL IMMUNITY TO MHV 68, A MURINE GAMMA HERPESVIRUS
    MARCIA BLACKMAN; Fiscal Year: 2008
    ..In Aim 3, we will build on this information and use this well-characterized viral model as a "proof of principal" test for epitope-based vaccination strategies for the gamma-herpesviruses. [unreadable] [unreadable] [unreadable]..
  25. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  26. CD8+ T Cells Control Autoimmunity In EAE
    Hong Jiang; Fiscal Year: 2007
    ..Thus, the studies will provide theoretical basis for clinical application of this regulatory pathway in prevention and treatment of multiple sclerosis in man. [unreadable] [unreadable] [unreadable]..
  27. B CLL SUBGROUPS--MATURATION STAGE AND GENE EXPRESSION
    Nicholas Chiorazzi; Fiscal Year: 2004
    ..Hopefully, these differences will provide important basic and clinical insights into this disease and suggest future prognostic and therapeutic approaches to it. ..
  28. CHROMOSOME ALTERATIONS IN BARRETT'S ESOPHAGUS
    THOMAS PAULSON; Fiscal Year: 2005
    ..The results from these analyses will be of interest to both clinical and basic researchers in studying EA and other cancers. ..
  29. EBV and HPV: Oral Infection, Persistence & Pathogenesis
    DENNIS WALLING; Fiscal Year: 2003
    ..abstract_text> ..
  30. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  31. Astrocytes As Multipotent Stem Cells
    ERIC LAYWELL; Fiscal Year: 2003
    ....
  32. CD8+ T CELL RECOGNITION OF TH1 AND TH2
    Hong Jiang; Fiscal Year: 2003
    ..We will determine if this difference uniquely applies to the recognition of TCR peptide presented by Qa-1 or if CD8+ T cells specific for other peptides presented by MHC class I molecules also distinguish between TH1 and TH2 targets. ..
  33. 3D Woven Scaffolds for Tissue Engineering
    Farshid Guilak; Fiscal Year: 2003
    ..An improved level of biomechanical function will increase the level of success in the engineered repair of various tissues of the musculoskeletal system as well as other organ systems of the body. ..
  34. Molecular Mechanisms of Aging: 33rd Ann. Mtg. of AGE
    Norman Wolf; Fiscal Year: 2004
    ..Dr. Bickford has investigated hotel accommodations there and has found that both the Radisson and the Hilton offer excellent accommodations for the meeting. She is negotiating with both. ..
  35. TARGETED DELIVERY OF GENES TO ANGIOGENIC VASCULATURE
    Renata Pasqualini; Fiscal Year: 2004
    ..Given that our peptides also target angiogenic vasculature in the retina, these advances are also likely to extend the potential for neovasculature-specific gene therapy targeting to other diseases involving angiogenesis. ..
  36. Ciprofloxacin resistance and compensatory Mutations.
    Julian Adams; Fiscal Year: 2004
    ..Knowledge of the biochemical and physiological basis of compensatory mutations may allow the design of strategies to reduce or counteract their role in increasing the prevalence of antibiotic resistant mutations. ..
  37. VASCULAR CELL CATIONIC AMINO ACID TRANSPORT & METABOLISM
    ANDREW SCHAFER; Fiscal Year: 2002
    ..Elucidation of the molecular mechanisms that modulate the response to vascular injury will provide new rational targets for therapeutic intervention. ..
  38. B220+DN Tcells in Mucosal Tolerance and Inflammation
    ABDEL HAMAD; Fiscal Year: 2005
    ..In addition, they lay the groundwork for the analysis of B220+ DN T cell in healthy individuals and IBD patients. ..
  39. FTI Biology & Treatment of Myeloproliferative Disorders
    Jason Gotlib; Fiscal Year: 2005
    ..If clinically efficacious, further studies of R115777 in MPDs will be warranted...
  40. DOSAGE COMPENSATION IN MAMMALS: X-INACTIVATION
    STANLEY GARTLER; Fiscal Year: 2004
    ..Such border shifting may have evolutionary implications for genome organization. We propose an extensive test of this model. ..
  41. Human Resistance to Herpes Simplex Virus Infections
    Christine Posavad; Fiscal Year: 2005
    ..The data generated will be relevant to designing and evaluating strategies for HSV-2 preventative vaccines and immunotherapy. ..
  42. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  43. REGULATION OF THE TYPE II TGF -B- RECEPTOR
    ARNOLD RIZZINO; Fiscal Year: 2002
    ..Together, Dr. Rizzino feels that these studies will not only help determine how the TbetaR-II gene is regulated normally, but they will also provide the groundwork for understanding why this gene is aberrantly expressed in tumors. ..
  44. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  45. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  46. Spindle Formation and Function in Oocytes and Early Embryos
    Keith E Latham; Fiscal Year: 2010
    ....
  47. EFFECT OF CLONING IN EPIGENETIC INHERITANCE IN MICE
    Keith Latham; Fiscal Year: 2004
    ..These studies are relevant to problems of human infertility and a variety of diseases involving inappropriate epigenetic regulation of gene function. ..
  48. DEVELOPMENT OF NOVEL ANTIFUNGAL ANTIPC NATURAL PRODUCTS
    Mitchell Avery; Fiscal Year: 2001
    ....
  49. Ras-PI3K Pathway in Nf1-/-Hematopoiesis and Leukemia
    David Ingram; Fiscal Year: 2007
    ..abstract_text> ..
  50. CHRONIC PROSTATITIS COLLABORATIVE CLINICAL STUDIES
    Richard Alexander; Fiscal Year: 2007
    ..abstract_text> ..
  51. EIAV Control by High Avidity Rev-Specific CTL Clones
    ROBERT MEALEY; Fiscal Year: 2005
    ..The information obtained from the proposed studies should have implications for HIV-1 immunotherapy and vaccine design, where experiments of this type may be more difficult. ..
  52. Mucosal and Peripheral NK-T Cells in HIV Infection
    Otto Yang; Fiscal Year: 2007
    ..Exploring NK-T cells in HIV-1 infection could there have a high impact on the development of new strategies in prevention and treatment. [unreadable] [unreadable]..
  53. Contrasting Properties of Integrin Cytoplasmic Domains
    Renata Pasqualini; Fiscal Year: 2004
    ..A better understanding of angiogenesis and new ways of manipulating such process in physiological and pathological situations may result from this work. ..
  54. Preimplantation Embryo Blastomere Fragmentation
    Keith Latham; Fiscal Year: 2006
    ..Last, because studies such as those proposed cannot be conducted in the human, the proposed studies will provide unprecedented data regarding the relationship between fragmentation and viability. ..
  55. Training in Biomolecular and Tissue Engineering
    Farshid Guilak; Fiscal Year: 2007
    ..We will also take advantage of several long-standing and successful initiatives at Duke University to enhance the recruitment of underrepresented minorities as part of this post-doctoral training program. ..
  56. ASSESSMENT OF CLINICAL RETINOID ACTIVITY IN LEUKEMIA
    Robert Gallagher; Fiscal Year: 2009
    ..Accomplishment of these aims may define a novel mechanism of gene-targeted drug resistance and provide greater understanding of clonal evolution in a therapeutic setting leading to relapse. ..
  57. Vaccine Development: Purinergic Agonists as Adjuvants
    Richard Granstein; Fiscal Year: 2005
    ..5) This work may provide insights into the physiological processes that modulate the immune response and further our understanding of the mechanisms of established adjuvants. ..
  58. Insulin Producing Cells from Amniotic Stem Cells for Diabetes Therapy
    Anthony Atala; Fiscal Year: 2009
    ..Successful development of an abundant source of transplantable insulin producing cells potentially would have a profound impact on the treatment of a major public health problem. ..
  59. Molecular Mechanisms Regulating Drosophila Ovarian Germline Stem Cells
    Ting Xie; Fiscal Year: 2010
    ..Therefore, this proposed study will surely contribute to a better understanding of stem cell biology, cancer formation and aging as well as to using stem cells in future regenerative medicine. ..
  60. Mycobacterium tuberculosis-reactive thymocytes
    MARIELLE GOLD; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  61. GENE SPECIFIC MUTATIONS IN SKIN INDUCED BY PEROXYNITRITE
    Fredika Robertson; Fiscal Year: 2001
    ..The hypothesis driving this research is that NO and peroxynitrite are mutagenic, and could be important contributors to oxidative DNA damage and mutation in human epidermal keratinocytes at sites of inflammation. ..
  62. TLS and TLS Fusion Proteins in Leukemia
    Liu Yang; Fiscal Year: 2005
    ..It is envisioned that successful completion of this project will unveil potential therapeutic targets in the treatment of human leukemias characterized by the t(16;21) translocation. ..
  63. Survivin expression and cancer cell drug resistance
    Fengzhi Li; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  64. PEM HOMEOBOX GENE
    Miles Wilkinson; Fiscal Year: 2002
    ..An understanding of the role of Pem in reproductive function may lead to advances in the areas of fertility and birth control. ..
  65. CHONDROGENESIS AND HISTONE MODIFICATION ENZYMES
    Liu Yang; Fiscal Year: 2007
    ....
  66. Eosinophil Granule Proteins and Their Functions
    Gerald Gleich; Fiscal Year: 2006
    ..Overall, these studies will expand our knowledge of the molecules composing the eosinophil granule and provide new tools to dissect eosinophil function in disease. ..
  67. THE HYPEREOSINOPHILIC SYNDROMES AND MEPOLIZUMAB
    Gerald Gleich; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  68. BIOENGINEERING OF BLADDER TISSUES
    Anthony Atala; Fiscal Year: 2003
    ..In this aim we will attempt to determine the phenotypic and functional characteristics, over time, of the bioengineered bladder tissues, as compared to controls. ..