jurkat cells

Summary

Summary: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.

Top Publications

  1. pmc STIM1, an essential and conserved component of store-operated Ca2+ channel function
    Jack Roos
    Torrey Pines Therapeutics, Inc, La Jolla, CA 92037, USA
    J Cell Biol 169:435-45. 2005
  2. pmc Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF
    V A Fadok
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Clin Invest 101:890-8. 1998
  3. pmc Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation
    Young Sik Cho
    Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cell 137:1112-23. 2009
  4. ncbi Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes
    Olivier Micheau
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Cell 114:181-90. 2003
  5. pmc C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells
    C A Ogden
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Exp Med 194:781-95. 2001
  6. pmc Inefficient human immunodeficiency virus replication in mobile lymphocytes
    Marion Sourisseau
    Groupe Virus et Immunité, URA CNRS 1930, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Virol 81:1000-12. 2007
  7. pmc Epigenetic regulation of HIV-1 latency by cytosine methylation
    Steven E Kauder
    Gladstone Institute of Virology and Immunology, San Francisco, California, United States of America
    PLoS Pathog 5:e1000495. 2009
  8. ncbi Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule
    N Holler
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nat Immunol 1:489-95. 2000
  9. pmc Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq data
    Raja Jothi
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 36:5221-31. 2008
  10. pmc Cell-cell spread of human immunodeficiency virus type 1 overcomes tetherin/BST-2-mediated restriction in T cells
    Clare Jolly
    MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, Windeyer Building, London, United Kingdom
    J Virol 84:12185-99. 2010

Detail Information

Publications449 found, 100 shown here

  1. pmc STIM1, an essential and conserved component of store-operated Ca2+ channel function
    Jack Roos
    Torrey Pines Therapeutics, Inc, La Jolla, CA 92037, USA
    J Cell Biol 169:435-45. 2005
    ..We propose that STIM1, a ubiquitously expressed protein that is conserved from Drosophila to mammalian cells, plays an essential role in SOC influx and may be a common component of SOC and CRAC channels...
  2. pmc Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF
    V A Fadok
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Clin Invest 101:890-8. 1998
    ..Therefore, it is likely that resolution of inflammation depends not only on the removal of apoptotic cells but on active suppression of inflammatory mediator production. Disorders in either could result in chronic inflammatory diseases...
  3. pmc Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation
    Young Sik Cho
    Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Cell 137:1112-23. 2009
    ..Our findings suggest that RIP3 controls programmed necrosis by initiating the pronecrotic kinase cascade, and that this is necessary for the inflammatory response against virus infections...
  4. ncbi Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes
    Olivier Micheau
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Cell 114:181-90. 2003
    ..Thus, TNFR1-mediated-signal transduction includes a checkpoint, resulting in cell death (via complex II) in instances where the initial signal (via complex I, NF-kappa B) fails to be activated...
  5. pmc C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells
    C A Ogden
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Exp Med 194:781-95. 2001
    ....
  6. pmc Inefficient human immunodeficiency virus replication in mobile lymphocytes
    Marion Sourisseau
    Groupe Virus et Immunité, URA CNRS 1930, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Virol 81:1000-12. 2007
    ..Altogether, these results indicate that cell-to-cell transfer is the predominant mode of HIV spread and help to explain why this virus replicates so efficiently in lymphoid organs...
  7. pmc Epigenetic regulation of HIV-1 latency by cytosine methylation
    Steven E Kauder
    Gladstone Institute of Virology and Immunology, San Francisco, California, United States of America
    PLoS Pathog 5:e1000495. 2009
    ..Two CpG islands flank the HIV-1 transcription start site and are methylated in latently infected Jurkat cells and primary CD4+ T cells...
  8. ncbi Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule
    N Holler
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nat Immunol 1:489-95. 2000
    ..Thus, Fas, TRAIL and TNF receptors can initiate cell death by two alternative pathways, one relying on caspase-8 and the other dependent on the kinase RIP...
  9. pmc Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq data
    Raja Jothi
    Laboratory of Molecular Immunology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 36:5221-31. 2008
    ..Using tag density as an indicator of DNA-binding affinity, we have identified core residues within the NRSF and CTCF binding sites that are critical for a stronger DNA binding...
  10. pmc Cell-cell spread of human immunodeficiency virus type 1 overcomes tetherin/BST-2-mediated restriction in T cells
    Clare Jolly
    MRC Centre for Medical Molecular Virology, Division of Infection and Immunity, University College London, Windeyer Building, London, United Kingdom
    J Virol 84:12185-99. 2010
    ..We found that Vpu-defective HIV-1 appeared to disseminate more efficiently by cell-to-cell contact between Jurkat cells under conditions where tetherin restricted cell-free virion release...
  11. pmc Down-modulation of mature major histocompatibility complex class II and up-regulation of invariant chain cell surface expression are well-conserved functions of human and simian immunodeficiency virus nef alleles
    Michael Schindler
    Department of Virology, Universitatsklinikum, 89081 Ulm, Germany
    J Virol 77:10548-56. 2003
    ..Overall, our results suggest that the ability of Nef to interfere with MHC-II antigen presentation might play a role in AIDS pathogenesis...
  12. pmc Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells
    Theodora Hatziioannou
    Aaron Diamond AIDS Research Center and Rockefeller University, New York, New York 10021, USA
    J Virol 79:176-83. 2005
    ..Specifically, by using normal and CypA-deficient Jurkat cells, we demonstrate that the presence of CypA in the target and not the virus-producing cell enhances HIV-1 ..
  13. pmc Transcriptional interference antagonizes proviral gene expression to promote HIV latency
    Tina Lenasi
    Department of Medicine, Rosalind Russell Medical Research Center, University of California San Francisco, San Francisco, CA 94143, USA
    Cell Host Microbe 4:123-33. 2008
    ..Collectively, our findings suggest that TI contributes significantly to HIV latency and should be considered when attempting to purge the latent reservoir...
  14. ncbi The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain
    M A Degli-Esposti
    Department of Biochemistry, Immunex Corporation, Seattle, Washington 98101, USA
    Immunity 7:813-20. 1997
    ..Like TRAIL-R1 and TRAIL-R2, this receptor shows widespread tissue expression. The human TRAIL-R4 gene has been mapped to chromosome 8p22-21, clustered with three other TRAIL receptors...
  15. ncbi A strain-promoted [3 + 2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems
    Nicholas J Agard
    Department of Chemistry, Howard Hughes Medical Institute, University of California, and Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    J Am Chem Soc 126:15046-7. 2004
    ..The utility of the reaction was demonstrated by selective modification of biomolecules in vitro and on living cells, with no apparent toxicity...
  16. doi Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71
    Yorihiro Nishimura
    Department of Virology II, National Institute of Infectious Diseases, Musashimurayama shi, Tokyo, Japan
    Nat Med 15:794-7. 2009
    ..The identification of PSGL-1 as a receptor for EV71 sheds new light on a role for PSGL-1-positive leukocytes in cell tropism and pathogenesis during the course of HFMD and other EV71-mediated diseases...
  17. pmc DNA specificity determinants associate with distinct transcription factor functions
    Peter C Hollenhorst
    Huntsman Cancer Institute, Department of Oncological Sciences, University of Utah, Salt Lake City, Utah, United States of America
    PLoS Genet 5:e1000778. 2009
    ..These findings demonstrate that diversity of DNA binding motifs may enable variable transcription factor function at different genomic sites...
  18. pmc CoREST is an integral component of the CoREST- human histone deacetylase complex
    A You
    Department of Molecular and Cellular Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 98:1454-8. 2001
    ....
  19. ncbi A receptor for phosphatidylserine-specific clearance of apoptotic cells
    V A Fadok
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    Nature 405:85-90. 2000
    ....
  20. ncbi Receptor-selective mutants of apoptosis-inducing ligand 2/tumor necrosis factor-related apoptosis-inducing ligand reveal a greater contribution of death receptor (DR) 5 than DR4 to apoptosis signaling
    Robert F Kelley
    Department of Protein Engineering, Genentech, Inc, South San Francisco, California 94080, USA
    J Biol Chem 280:2205-12. 2005
    ..These results suggest that DR5 may contribute more than DR4 to Apo2L/TRAIL-induced apoptosis in cancer cells that express both death receptors...
  21. doi Cucurbitacin B induces cell cycle arrest, apoptosis and autophagy associated with G actin reduction and persistent activation of cofilin in Jurkat cells
    Jun shan Zhu
    Department of Immunobiology, Jinan University, Guangzhou, PR China
    Pharmacology 89:348-6. 2012
    The present study aimed to explore the antitumor effect and action mechanism of cucurbitacin B (CuB) on human T-cell leukemia Jurkat cells.
  22. ncbi Annexin I is an endogenous ligand that mediates apoptotic cell engulfment
    Swathi Arur
    Center for Vascular Biology, Department of Physiology, University of Connecticut School of Medicine, 263 Farmington Avenue, Farmington, CT 06030, USA
    Dev Cell 4:587-98. 2003
    ..These results provide novel mechanistic insights into how apoptotic cells are removed and may explain a pathogenic mechanism of chronic inflammatory diseases where annexin I autoantibodies have been described...
  23. ncbi Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts
    V A Fadok
    Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    J Biol Chem 276:1071-7. 2001
    ..These observations directly demonstrate that loss of phospholipid asymmetry and PS expression is required for phagocyte engulfment of apoptotic cells and imply a critical, if not obligatory, role for PS recognition in the uptake process...
  24. pmc A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication
    Man Lung Yeung
    Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland 20892 0460, USA
    J Biol Chem 284:19463-73. 2009
    ..We discuss the similarities and differences between our shRNA findings for HIV-1 using a spreading infection assay in human Jurkat T-cells and results from other investigators who used siRNA-based screenings in HeLa or 293T cells...
  25. pmc Evidence for direct involvement of the capsid protein in HIV infection of nondividing cells
    Masahiro Yamashita
    Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    PLoS Pathog 3:1502-10. 2007
    ..Our data suggest that CA is directly involved at some step in the viral life cycle that is important for infection of nondividing cells...
  26. ncbi FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation
    T Kataoka
    Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland
    J Immunol 161:3936-42. 1998
    ..e., doxorubicin, etoposide, and vincristine) and gamma irradiation was not affected by FLIP or the absence of Fas, indicating that these treatments can induce cell death in a Fas-independent and FLIP-insensitive manner...
  27. doi Role of microRNA-143 in Fas-mediated apoptosis in human T-cell leukemia Jurkat cells
    Yukihiro Akao
    Department of Medical Oncology, Gifu International Institute of Biotechnology, Kakamigahara, Gifu 504 0838, Japan
    Leuk Res 33:1530-8. 2009
    ..Furthermore, the transfection of Jurkat cells with mature miR-143 induced a significant growth suppression and enhancement of CH-11-induced apoptosis...
  28. pmc Actin retrograde flow and actomyosin II arc contraction drive receptor cluster dynamics at the immunological synapse in Jurkat T cells
    Jason Yi
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 23:834-52. 2012
    ..Thus actin retrograde flow and actomyosin II arc contraction coordinately drive receptor cluster dynamics at the immunological synapse...
  29. ncbi Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells
    Changjiang Weng
    Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China 100080
    J Biol Chem 280:10491-500. 2005
    ..to cycloheximide-induced Mcl-1 dilapidation, TRAIL did not activate proteasomal degradation of Mcl-1 in Jurkat cells. We further established for the first time that the C-terminal domain of Mcl-1 became proapoptotic as a result ..
  30. ncbi Effects of Bcl-2 levels on Fas signaling-induced caspase-3 activation: molecular genetic tests of computational model predictions
    Fei Hua
    Center for Cancer Research and Biological Engineering Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    J Immunol 175:985-95. 2005
    ..Finally, we demonstrate that the relative dominance of type I vs type II pathways can be switched by varying particular signaling component levels without changing network structure...
  31. pmc T cell receptor internalization from the immunological synapse is mediated by TC21 and RhoG GTPase-dependent phagocytosis
    Nuria Martínez-Martín
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, 28049 Madrid, Spain
    Immunity 35:208-22. 2011
    ..Therefore, TC21 and RhoG dependence underlie the existence of a common phagocytic mechanism that drives TCR internalization from the IS together with its peptide-MHC ligand...
  32. doi Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission
    Stefanie Sowinski
    Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, SW7 2AZ, UK
    Nat Cell Biol 10:211-9. 2008
    ..These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize...
  33. pmc Initiation of apoptosis by granzyme B requires direct cleavage of bid, but not direct granzyme B-mediated caspase activation
    V R Sutton
    Cancer Immunology Laboratory, Peter MacCallum Cancer Institute, Melbourne 8006, Australia
    J Exp Med 192:1403-14. 2000
    ..These results clearly placed Bid cleavage upstream of mitochondrial Bcl-2. In granzyme B-treated Jurkat cells, endogenous Bid cleavage and loss of mitochondrial membrane depolarization occurred despite caspase ..
  34. ncbi A role for tumor necrosis factor receptor-2 and receptor-interacting protein in programmed necrosis and antiviral responses
    Francis Ka Ming Chan
    Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:51613-21. 2003
    ..To ascertain the physiological significance of RIP and programmed necrosis, we infected Jurkat cells with vaccinia virus (VV) and found that VV-infected cells underwent programmed necrosis in response to TNF, but ..
  35. pmc Copper-free click chemistry for dynamic in vivo imaging
    Jeremy M Baskin
    Department of Chemistry, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:16793-7. 2007
    ..With this technique, we studied the dynamics of glycan trafficking and identified a population of sialoglycoconjugates with unexpectedly rapid internalization kinetics...
  36. pmc Differential inhibition of TRAIL-mediated DR5-DISC formation by decoy receptors 1 and 2
    Delphine Merino
    INSERM, U517, Univ Bourgogne, Dijon F 21000, France
    Mol Cell Biol 26:7046-55. 2006
    ..In addition, DcR2 prevents DR4 recruitment within the DR5 DISC. The specificity of DcR1- and DcR2-mediated TRAIL inhibition reveals an additional level of complexity for the regulation of TRAIL signaling...
  37. ncbi Prostratin antagonizes HIV latency by activating NF-kappaB
    Samuel A Williams
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, California 94141, USA
    J Biol Chem 279:42008-17. 2004
    ..These findings provide new insights into the molecular pathway through which prostratin antagonizes HIV latency highlighting a central role for the action of NF-kappaB...
  38. ncbi Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses
    S Liu
    Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 402:676-81. 1999
    ..Thus, the tight association of paxillin with the alpha4 tail leads to distinct biochemical and biological responses to integrin-mediated cell adhesion...
  39. doi T cell costimulation via the integrin VLA-4 inhibits the actin-dependent centralization of signaling microclusters containing the adaptor SLP-76
    Ken Nguyen
    Program in Immunology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    Immunity 28:810-21. 2008
    ..These data suggest a widely applicable model of costimulation, in which integrins promote sustained signaling by attenuating cytoskeletal movements that drive the centralization and inactivation of SLP-76 microclusters...
  40. pmc Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family
    M A Degli-Esposti
    Department of Biochemistry and the Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA
    J Exp Med 186:1165-70. 1997
    ..Moreover, unlike TRAIL-R1 and -R2, in a transient overexpression system TRAIL-R3 does not induce apoptosis...
  41. ncbi Rapid, noninflammatory and PS-dependent phagocytic clearance of necrotic cells
    U A Hirt
    Faculty of Biology, University of Konstanz, X911, D 78457 Konstanz, Germany
    Cell Death Differ 10:1156-64. 2003
    ..We demonstrate that this can occur independently of calcium signals, and that surface phosphatidylserine is essential for the uptake of necrotic cells by both human macrophages and murine microglia...
  42. ncbi Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling
    K Zhao
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University, California 94305 5323, USA
    Cell 95:625-36. 1998
    ..This work indicates that membrane signals control the activity of the mammalian SWI/SNF or BAF complex and demonstrates a direct interface between signaling and chromatin regulation...
  43. pmc Sialic acid-binding lectin (leczyme) induces caspase-dependent apoptosis-mediated mitochondrial perturbation in Jurkat cells
    Takeo Tatsuta
    Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiology, Tohoku Pharmaceutical University, Aoba ku, Sendai 981 8558, Japan
    Int J Oncol 43:1402-12. 2013
    ..SBL selectively kills tumor cells, is able to exhibit cytotoxicity regardless of P-glycoprotein expression and has potential as an alternative to conventional DNA-damaging anticancer drugs. ..
  44. pmc Preligand assembly domain-mediated ligand-independent association between TRAIL receptor 4 (TR4) and TR2 regulates TRAIL-induced apoptosis
    Lauren Clancy
    Department of Pathology, Immunology and Virology Program, University of Massachusetts Medical School, Worcester, 01655, USA
    Proc Natl Acad Sci U S A 102:18099-104. 2005
    ..We propose that TR4 is a "regulatory" rather than "decoy" receptor that inhibits apoptosis signaling by TRAIL through this previously uncharacterized ligand-independent mechanism...
  45. pmc T-cell receptor-induced JNK activation requires proteolytic inactivation of CYLD by MALT1
    Jens Staal
    Department of Molecular Biomedical Research, Unit of Molecular Signal Transduction in Inflammation, VIB, Zwijnaarde, Ghent, Belgium
    EMBO J 30:1742-52. 2011
    ..These results indicate a novel role for MALT1 proteolytic activity in TCR-induced JNK activation and reveal CYLD cleavage as the underlying mechanism...
  46. pmc Ezrin tunes T-cell activation by controlling Dlg1 and microtubule positioning at the immunological synapse
    Rémi Lasserre
    Institut Pasteur, Department of Immunology, Lymphocyte Cell Biology Unit, Paris, France
    EMBO J 29:2301-14. 2010
    ..Moreover, they underscore the importance of ezrin and Dlg1 in the regulation of NF-AT activation through p38...
  47. pmc Persistence of cooperatively stabilized signaling clusters drives T-cell activation
    Stephen C Bunnell
    Laboratory of Cellular and Molecular Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland, USA
    Mol Cell Biol 26:7155-66. 2006
    ..These data indicate that multivalent cooperative interactions stabilize these persistent signaling clusters, which may correspond to the functional complexes predicted by kinetic proofreading models of T-cell activation...
  48. ncbi Enterovirus 71 infection induces Fas ligand expression and apoptosis of Jurkat cells
    Lien Cheng Chen
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    J Med Virol 78:780-6. 2006
    ..Viable viruses were recovered from both the culture supernatant and the cell lysate of Jurkat cells after EV71 infection...
  49. pmc The Ca2+-dependent phosphatase calcineurin controls the formation of the Carma1-Bcl10-Malt1 complex during T cell receptor-induced NF-kappaB activation
    Lysann Palkowitsch
    Institute of Physiological Chemistry, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
    J Biol Chem 286:7522-34. 2011
    ..In summary, the evidence provided here argues for a previously unanticipated role of calcineurin in CBM complex formation as a molecular basis of the inhibitory function of CsA or FK506 on TCR-induced NF-κB activity...
  50. pmc CD5 negatively regulates the T-cell antigen receptor signal transduction pathway: involvement of SH2-containing phosphotyrosine phosphatase SHP-1
    J J Perez-Villar
    Immunology and Inflammation Drug Discovery, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543, USA
    Mol Cell Biol 19:2903-12. 1999
    ..with the Src homology 2 (SH2) domain containing hematopoietic phosphotyrosine phosphatase SHP-1 in both Jurkat cells and normal phytohemagglutinin-expanded T lymphoblasts...
  51. pmc A hierarchical role for classical pathway complement proteins in the clearance of apoptotic cells in vivo
    P R Taylor
    Rheumatology Section, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, United Kingdom
    J Exp Med 192:359-66. 2000
    ..Apoptotic cells are thought to be a major source of the autoantigens of SLE, and impairment of their removal by complement may explain the link between hereditary complement deficiency and the development of SLE...
  52. ncbi DNA damage and apoptosis in hydrogen peroxide-exposed Jurkat cells: bolus addition versus continuous generation of H(2)O(2)
    Alexandra Barbouti
    Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina, Greece
    Free Radic Biol Med 33:691-702. 2002
    ..These observations stress that, apart from the apparent genotoxic and proapoptotic effects of H(2)O(2), it can also exert antiapoptotic actions when present, even at low concentrations, during the execution of apoptosis...
  53. ncbi FADD/MORT1 and caspase-8 are recruited to TRAIL receptors 1 and 2 and are essential for apoptosis mediated by TRAIL receptor 2
    M R Sprick
    Tumor Immunology Program, German Cancer Research Center DKFZ Heidelberg
    Immunity 12:599-609. 2000
    ..FADD/MORT1- and caspase-8-deficient Jurkat cells expressing only TRAIL-R2 were resistant to TRAIL-induced apoptosis...
  54. pmc CD3/CD28 costimulation-induced NF-kappaB activation is mediated by recruitment of protein kinase C-theta, Bcl10, and IkappaB kinase beta to the immunological synapse through CARMA1
    Donghai Wang
    Department of Microbiology and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, SUNY, Buffalo, New York 14214, USA
    Mol Cell Biol 24:164-71. 2004
    ..Together, these results indicate that CARMA1 mediates CD3/CD28 costimulation-induced NF-kappaB activation by recruiting downstream signaling components into the immunological synapse...
  55. ncbi Calcium-activated potassium channels sustain calcium signaling in T lymphocytes. Selective blockers and manipulated channel expression levels
    C M Fanger
    Department of Physiology and Biophysics, University of California, Irvine, California 92697-4561, USA
    J Biol Chem 276:12249-56. 2001
    ..Thus, K(Ca) channels play a vital role in T cell Ca(2+) signaling...
  56. pmc Phosphatidylserine-dependent ingestion of apoptotic cells promotes TGF-beta1 secretion and the resolution of inflammation
    Mai Lan N Huynh
    Department of Pulmonary and Critical Care Medicine, University of Colorado Health Sciences Center, Denver, Colorado, USA
    J Clin Invest 109:41-50. 2002
    ..In conclusion, apoptotic cell recognition and clearance, via exposure of PS and ligation of its receptor, induce TGF-beta1 secretion, resulting in accelerated resolution of inflammation...
  57. ncbi FOXP3 controls regulatory T cell function through cooperation with NFAT
    Yongqing Wu
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309, USA
    Cell 126:375-87. 2006
    ..Thus by switching transcriptional partners, NFAT converts the acute T cell activation program into the suppressor program of Tregs...
  58. ncbi Activation of IKK by TNFalpha requires site-specific ubiquitination of RIP1 and polyubiquitin binding by NEMO
    Chee Kwee Ea
    Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Cell 22:245-57. 2006
    ..These results reveal the biochemical mechanism underlying the essential signaling function of NEMO and provide direct evidence that signal-induced site-specific ubiquitination of RIP1 is required for IKK activation...
  59. pmc STIM1 has a plasma membrane role in the activation of store-operated Ca(2+) channels
    Maria A Spassova
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 103:4040-5. 2006
    ..As a cell surface signaling protein, STIM1 represents a key pharmacological target to control fundamental Ca(2+)-regulated processes including secretion, contraction, metabolism, cell division, and apoptosis...
  60. ncbi The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes
    Lakshmanan Ganesh
    Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 4502, MSC 3005, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nature 426:853-7. 2003
    ..Through its effects on the proteasome, Murr1 acts as a genetic restriction factor that inhibits HIV-1 replication in lymphocytes, which could contribute to the regulation of asymptomatic HIV infection and the progression of AIDS...
  61. pmc T cell antigen receptor signaling and immunological synapse stability require myosin IIA
    Tal Ilani
    Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, New York, USA
    Nat Immunol 10:531-9. 2009
    ..Thus, T cell antigen receptor signaling and the subsequent formation of immunological synapses are active processes dependent on myosin IIA...
  62. ncbi Nef triggers a transcriptional program in T cells imitating single-signal T cell activation and inducing HIV virulence mediators
    A Simmons
    MRC Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    Immunity 14:763-77. 2001
    ..New genes associated with Nef include CDK9, the induction of which enhances Tat function. Thus, Nef acts as a master switch early in the viral life cycle, forcing an environment conducive to dynamic viral production...
  63. ncbi TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex
    Nicolas Blanchard
    Institut National de la Sante et de la Recherche Medicale, Unité 520, Institut Curie, Paris, France
    J Immunol 168:3235-41. 2002
    ....
  64. pmc Analysis of microarray data using Z score transformation
    Chris Cheadle
    DNA Array Unit, Research Resources Branch, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21221, USA
    J Mol Diagn 5:73-81. 2003
    ..The results provided by these methods can be as rigorous and are no more arbitrary than other test methods, and, in addition, they have the advantage that they can be easily adapted to standard spreadsheet programs...
  65. ncbi Glucocorticoids engage different signal transduction pathways to induce apoptosis in thymocytes and mature T cells
    Dapeng Wang
    Molecular Immunology, Institute for Virology and Immunobiology, University of Wurzburg, Wurzburg, Germany
    J Immunol 176:1695-702. 2006
    ..Collectively, the apoptotic program induced by GCs comprises cell type-specific as well as common features...
  66. pmc Predominant mode of human immunodeficiency virus transfer between T cells is mediated by sustained Env-dependent neutralization-resistant virological synapses
    Ping Chen
    Department of Medicine, Division of Infectious Diseases, Immunology Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Virol 81:12582-95. 2007
    ..Future studies will discern to what extent this massive viral transfer contributes to productive infection or viral dissemination through the migration of virus-carrying T cells...
  67. ncbi Caspase-3 is the primary activator of apoptotic DNA fragmentation via DNA fragmentation factor-45/inhibitor of caspase-activated DNase inactivation
    B B Wolf
    Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121, USA
    J Biol Chem 274:30651-6. 1999
    ..Together, these data suggest that caspase-3 is the primary inactivator of DFF45/ICAD and therefore the primary activator of apoptotic DNA fragmentation...
  68. doi The proteolytic activity of the paracaspase MALT1 is key in T cell activation
    Fabien Rebeaud
    Department of Biochemistry, University of Lausanne, CH 1066 Epalinges, Switzerland
    Nat Immunol 9:272-81. 2008
    ..Thus, the proteolytic activity of MALT1 is central to T cell activation, which suggests a possible target for the development of immunomodulatory or anticancer drugs...
  69. ncbi Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo
    P Pavan Kumar
    National Centre for Cell Science, Ganeshkhind, Pune 411007, India
    Mol Cell 22:231-43. 2006
    ....
  70. ncbi Rho-ROCK-dependent ezrin-radixin-moesin phosphorylation regulates Fas-mediated apoptosis in Jurkat cells
    Marylise Hebert
    Institut National de la Santé et de la Recherche Médicale U749, Faculte de Pharmacie, Chatenay Malabry, France
    J Immunol 181:5963-73. 2008
    ..We show herein that in Jurkat cells, not only ezrin but also moesin can associate with Fas...
  71. pmc Quantitative 3D video microscopy of HIV transfer across T cell virological synapses
    Wolfgang Hubner
    Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, New York, NY 10029, USA
    Science 323:1743-7. 2009
    ..Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis...
  72. ncbi Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA
    S Bauer
    Fred Hutchinson Cancer Research Center, Clinical Research Division, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 285:727-9. 1999
    ..These results define an activating immunoreceptor-MHC ligand interaction that may promote antitumor NK and T cell responses...
  73. pmc Identification of an N-terminal truncation of the NF-κB p65 subunit that specifically modulates ribosomal protein S3-dependent NF-κB gene expression
    Eric M Wier
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 287:43019-29. 2012
    ..Our findings thus uncover a new cytoplasmic function for the N-terminal domain of p65 and provide a novel strategy for selective inhibition of NF-κB gene transcription...
  74. doi Sulforaphane induces DNA single strand breaks in cultured human cells
    Piero Sestili
    Dipartimento di Scienze Biomolecolari, Via Maggetti, 21, Universita degli Studi di Urbino Carlo Bo, 61029 Urbino, PU, Italy
    Mutat Res 689:65-73. 2010
    ..Furthermore ROS were not formed in Jurkat cells pharmacologically depleted of respiring mitochondria (MRC-/Jurkat)...
  75. pmc Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells
    Maria Mittelbrunn
    Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernandez Almagro, 3 28029, Madrid, Spain
    Nat Commun 2:282. 2011
    ..Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis...
  76. pmc Genome-wide binding map of the HIV-1 Tat protein to the human genome
    Céline Marban
    Institut de Virologie, Universite de Strasbourg, Strasbourg, France
    PLoS ONE 6:e26894. 2011
    ..Interestingly, Tat binds to promoters of genes that, in Jurkat cells, are bound by the ETS1 transcription factor, the CBP histone acetyltransferase and/or are enriched for histone ..
  77. pmc ELL facilitates RNA polymerase II pause site entry and release
    Jung S Byun
    Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, Maryland 20892, USA
    Nat Commun 3:633. 2012
    ..Thus, ELL has an early and essential role during rapid high-amplitude gene expression that is required for both Pol II pause site entry and release...
  78. pmc Suberoylanilide hydroxamic acid reactivates HIV from latently infected cells
    Xavier Contreras
    Department of Medicine, University of California, San Francisco, California 94143, USA
    J Biol Chem 284:6782-9. 2009
    ..Thus SAHA, which is a Food and Drug Administration-approved drug, might be considered to accelerate the decay of the latent reservoir in HAART-treated infected humans...
  79. pmc STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx
    Jen Liou
    Department of Molecular Pharmacology, Stanford University Medical School, California 94305, USA
    Curr Biol 15:1235-41. 2005
    ..Our study suggests that STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(2+) store depletion to Ca(2+) influx...
  80. doi Altered let-7 expression in Myasthenia gravis and let-7c mediated regulation of IL-10 by directly targeting IL-10 in Jurkat cells
    Lin Jiang
    State Key Laboratory of Bioreactor Engineering and Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China
    Int Immunopharmacol 14:217-23. 2012
    ..We also showed that let-7c could regulate IL-10 expression in Jurkat cells. In summary, our results suggest that abnormal expression/regulation of microRNAs may contribute to or be ..
  81. pmc Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection
    Yorihiro Nishimura
    Department of Virology II, National Institute of Infectious Diseases, Musashimurayama shi, Tokyo, Japan
    PLoS Pathog 6:e1001174. 2010
    ..Thus, the results presented in this study reveal that tyrosine sulfation, but not O-glycosylation, in the N-terminal region of PSGL-1 may facilitate virus entry and replication of EV71 in leukocytes...
  82. pmc Heparan sulfate modification of the transmembrane receptor CD47 is necessary for inhibition of T cell receptor signaling by thrombospondin-1
    Sukhbir Kaur
    Laboratory of Pathology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20982, USA
    J Biol Chem 286:14991-5002. 2011
    ..Therefore, inhibition of T cell receptor signaling by thrombospondin-1 is mediated by CD47 and requires its modification at Ser(64)...
  83. pmc Successful targeting and disruption of an integrated reporter lentivirus using the engineered homing endonuclease Y2 I-AniI
    Martine Aubert
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    PLoS ONE 6:e16825. 2011
    ..This new technology offers the possibility of selectively disabling integrated HIV provirus within latently infected cells...
  84. ncbi CD69 acts downstream of interferon-alpha/beta to inhibit S1P1 and lymphocyte egress from lymphoid organs
    Lawrence R Shiow
    Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143, USA
    Nature 440:540-4. 2006
    ..These observations indicate that CD69 forms a complex with and negatively regulates S1P1 and that it functions downstream of IFN-alpha/beta, and possibly other activating stimuli, to promote lymphocyte retention in lymphoid organs...
  85. pmc Chronic lymphocytic leukemia cells receive RAF-dependent survival signals in response to CXCL12 that are sensitive to inhibition by sorafenib
    Davorka Messmer
    Moores Cancer Center, University of California San Diego, La Jolla, CA, USA
    Blood 117:882-9. 2011
    ..Moreover, sorafenib may have clinical activity for patients with CLL, particularly those with ZAP-70(+) CLL...
  86. pmc SKAP1 protein PH domain determines RapL membrane localization and Rap1 protein complex formation for T cell receptor (TCR) activation of LFA-1
    Monika Raab
    Department of Pathology, University of Cambridge, Cambridge, United Kingdom
    J Biol Chem 286:29663-70. 2011
    ..Further, N-terminal myr-tagged SKAP1 for membrane binding facilitated constitutive RapL membrane and Rap1 binding and effectively substituted for PI3K and TCR ligation in the activation of LFA-1 in T cells...
  87. ncbi Bryostatin-1 synergizes with histone deacetylase inhibitors to reactivate HIV-1 from latency
    Moises Perez
    Departamento de Biologia Celular, Fisiologia e Inmunologia, Cordoba, Spain
    Curr HIV Res 8:418-29. 2010
    ..Therefore, bryostatin-1, alone or in combination with HDAC inhibitors, could be used in HAART treated patients to validate the hypothesis that reactivating HIV-1 from latency could purge HIV-1 reservoirs...
  88. pmc Role of diacylglycerol kinase alpha in the attenuation of receptor signaling
    M A Sanjuan
    Department of Immunology and Oncology, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, E 28049 Madrid, Spain
    J Cell Biol 153:207-20. 2001
    ..This study is the first to describe DGKalpha as an integral component of the signaling cascades that link plasma membrane receptors to nuclear responses...
  89. ncbi RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion
    Esther M Lafuente
    Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:585-95. 2004
    ..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics...
  90. doi Hexamethylbisacetamide and disruption of human immunodeficiency virus type 1 latency in CD4(+) T cells
    Shailesh K Choudhary
    Departments of Medicine, University of North Carolina at Chapel Hill 27599 7435, USA
    J Infect Dis 197:1162-70. 2008
    ....
  91. ncbi CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation
    Olivier Gaide
    Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Nat Immunol 3:836-43. 2002
    ..Together, our data show that CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation and CD28 costimulation-dependent Jnk activation...
  92. pmc Isolation of a TRAIL antagonist from the serum of HIV-infected patients
    David J Schnepple
    Division of Infectious Diseases, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Biol Chem 286:35742-54. 2011
    ..Knockdown of TRAIL-s abrogates this resistance. We propose that TRAIL-s is a novel adaptive mechanism of apoptosis resistance acquired by HIV-infected cells to avoid their elimination by TRAIL-dependent effector mechanism...
  93. ncbi Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell lines
    Yoshinori Ishikawa
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Gene Med 8:646-53. 2006
    ..Here, to explore an alternative gene transfer methodology that may offer less risk of insertional mutagenesis, we employed the phiC31 integrase-based integration system using human T-cell lines, including the gammac-deficient ED40515(-)...
  94. ncbi Suppression of TNF-alpha-induced apoptosis by NF-kappaB
    D J Van Antwerp
    Laboratory of Genetics, Salk Institute, La Jolla, CA 92037, USA Jolla Institute for Allergy and Immu
    Science 274:787-9. 1996
    ..These findings suggest that a negative feedback mechanism results from TNF-alpha signaling in which NF-kappaB activation suppresses the signals for cell death...
  95. doi Dephosphorylation of Bcl-10 by calcineurin is essential for canonical NF-κB activation in Th cells
    Stefan Frischbutter
    German Rheumatism Research Center, a Leibniz Institute, Berlin, Germany
    Eur J Immunol 41:2349-57. 2011
    ..Together, our findings demonstrate that CaN functions as a critical signaling molecule during Th cell activation, regulating Bcl-10 phosphorylation and thereby NF-κB activation...
  96. pmc Dephosphorylation of Carma1 by PP2A negatively regulates T-cell activation
    Andrea C Eitelhuber
    Department of Cellular Signal Integration, Helmholtz Zentrum Munchen German Research Center for Environmental Health, Institute of Toxicology, Neuherberg, Germany
    EMBO J 30:594-605. 2011
    ..Thus, our data define PP2A-mediated dephosphorylation of Carma1 as a critical step to limit T-cell activation and effector cytokine production...
  97. pmc Global kinetic analysis of proteolysis via quantitative targeted proteomics
    Nicholas J Agard
    Department of Pharmaceutical Chemistry, University of California, San Francisco, 1700 4th Street, San Francisco, CA 94114, USA
    Proc Natl Acad Sci U S A 109:1913-8. 2012
    ..We believe this massively parallel and quantitative label-free approach to obtaining basic enzymological constants will facilitate the study of proteolysis and other posttranslational modifications in complex mixtures...
  98. pmc Differential cytokine regulation by NF-kappaB and AP-1 in Jurkat T-cells
    Hazem Khalaf
    Biology, Orebro Life Science Center, School of Science and Technology, Orebro University, SE 701 82 Orebro, Sweden
    BMC Immunol 11:26. 2010
    ..The aim of this study was to investigate the role of the transcription factors, AP-1 and NF-kappaB, in IL-6 and CXCL8 regulation in Jurkat T-cells...
  99. ncbi LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation
    W Zhang
    Section on Lymphocyte Signaling, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 5430, USA
    Immunity 9:239-46. 1998
    ..LAT is central to T cell activation and is a novel linker molecule shown to require targeting to membrane microdomains for signaling...
  100. ncbi Selective downmodulation of HLA-A and -B by Nef alleles from different groups of primate lentiviruses
    Anke Specht
    Institute of Virology, Universitatsklinikum, 89081 Ulm, Germany
    Virology 373:229-37. 2008
    ....
  101. ncbi PKCtheta cooperates with atypical PKCzeta and PKCiota in NF-kappaB transactivation of T lymphocytes
    Thomas Gruber
    Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Schoepfstrasse 41, A 6020 Innsbruck, Austria
    Mol Immunol 45:117-26. 2008
    ..Together, these data demonstrate that atypical PKCzeta/iota isotypes serve as direct downstream targets of PKCtheta in the signalling pathway leading to NF-kappaB activation in T lymphocytes...

Research Grants73

  1. Identifying Regulators of Oncogenic CARD11 in Diffuse Large B Cell Lymphoma
    Demauri Mackie; Fiscal Year: 2013
    ..of C9orf9 in the TCR-to-NF- B pathway, including characterizing the phenotype when C9orf9 is knocked down in Jurkat cells, confirming the location of C9orf9 in the TCR-to-NF- B pathway, and identifying critical regions or residues of ..
  2. Thrombomodulin Receptors on Immune Cells
    John Morser; Fiscal Year: 2013
    ..Lysates of Jurkat cells contain bands that interact with the lectin domain on a Western...
  3. Genetic and Immunological Impact of the HRES-1/Rab4 Locus in SLE
    Andras Perl; Fiscal Year: 2013
    ..targets CD4 for lysosomal degradation, while dominant-negative HRES-1/Rab4S27N has the opposite effect both in Jurkat cells and peripheral blood T cells...
  4. Identification of novel genes regulating metalloproteinase activity
    Andreas Herrlich; Fiscal Year: 2012
    ..initial screen that identified several inhibitors and activators of phorbol ester-induced TGFalpha cleavage in Jurkat cells. Knock-down of PKCzeta, an atypical protein kinase C isoform not activated by phorbol ester is identified as an ..
  5. Role of Mixed Lineage Kinase 3/SPRK in Stress Signaling
    AJAY NMN RANA; Fiscal Year: 2010
    ..and activate MLK3, (ii) MLK3 mediates TNF-a and ceramide-induced JNK activation, (iii) TNF-a treatment of Jurkat cells triggers TRAF2 association with MLK3, (vi) overexpression of MLK3 causes cell death, and (v) Retinoic acid ..
  6. A Method to Stop HIV Replication:Inhibition of Human Purine Utilizing Proteins
    Jesse J Kwiek; Fiscal Year: 2013
    ..labeling with amino acids in cell culture) to quantify purinome proteins induced by HIV-1 infection of THP1 and Jurkat cells (target identification);determine which purinome proteins are essential for HIV-1 replication (target ..
  7. Analysis of adaptor protein (LAT) in TCR signaling
    Weiguo Zhang; Fiscal Year: 2011
    ..Previous studies with LAT- deficient Jurkat cells demonstrate that LAT is essential for TCP-mediated signal transduction and LAT palmitoylation is required for ..
  8. Integrated Multi-scale Adhesive Dynamics Modeling of T-lymphocyte Homing
    Daniel A Hammer; Fiscal Year: 2013
    ..substrates;and 3) to compare our simulations with the adhesive behavior of engineered T- lymphocytes, including Jurkat cells and T cells from knock-out mice in which SLP-76 is deleted or altered, or in which diacylglycerol kinases (DGK) ..
  9. Immunomodulatory Function of Human T Cell Leukemia Virus Type 2 (HTLV-2)
    Mark A Beilke; Fiscal Year: 2012
    ..coli (or in stably transduced Jurkat cells) is a potent inducer of beta chemokines by peripheral blood mononuclear cells (PBMCs), and was observed to down-..
  10. Mechanisms of Immune Modulation by Abscisic Acid
    Josep Bassaganya Riera; Fiscal Year: 2010
    ..will: 1) Investigate the ability of ABA's isomers and enantiomers to activate PPAR? in reporter assays in Jurkat cells;2) Evaluate the specificity of pure ABA enantiomers as PPAR? ligands in binding assays;3) Use the most ..
  11. The role of SLP-76 phosphorylation in T cell development
    Martha S Jordan; Fiscal Year: 2011
    ..This hypothesis will be tested by 1) analyzing SLP-76 deficient Jurkat cells reconstituted with SLP-76 bearing mutations at one or multiple tyrosine phosphorylation sites, 2) analyzing SLP-..
  12. Immune Tolerance; Sigma Receptor as a Therapeutic Target
    Vadivel Ganapathy; Fiscal Year: 2003
    ..control) and sigma 1 receptor-negative (stable transfectants expressing antisense sigma 1 receptor mRNA) Jurkat cells. Specific Aim 2 is to identify the proteins in human placenta and in T lymphocytes that interact with sigma 1 ..
  13. INHIBITING TRANSACTIVATION OF HIV-1
    Robert Ricciardi; Fiscal Year: 1992
    ..under the control of a foreign promoter, will be cotransfected with an HIV LTR-CAT reporter plasmid in HeLa and Jurkat cells. Mutant tat plasmids which fail to transactivate will be cotransfected with the wt tat plasmid and the HIV LTR-..
  14. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2002
    ..complement, insulin) and the BCN-1 nuclear factor is also present in monkey kidney COS-7 cells and human Jurkat cells. He proposes three aims: 1. To characterize transcription factors which bind to the bcn-1 motif...
  15. Modulation of Glucocorticoid-Evoked Lymphocyte Apoptosis by BTG1
    RHEEM MEDH; Fiscal Year: 2007
    ..assays, and transient cotransfections of GR, BTG1 and a GR-driven luciferase reporter construct, pHHLuc in Jurkat cells. These studies will significantly advance our understanding of the mechanisms of T-cell apoptosis, and the ..
  16. PROTEINS BINDING TO THE HUMAN GAMMA INTERFERON PROMOTER
    Don Diamond; Fiscal Year: 1992
    ..Use of Jurkat cells as a model system is one approach through the introduction of vectors expressing tax and studying their effects ..
  17. Autoimmune vitiligo as a roadmap to melanoma therapy
    ISABELLE LE POOLE; Fiscal Year: 2007
    ..with gp100 or MART-1 will be cloned into retroviral vectors and expressed against the same background (in Jurkat cells) for further characterization of the relative contribution of the TCR to differential reactivity...
  18. Inhibition of CTL killing by HSV
    KEITH JEROME; Fiscal Year: 2005
    ..Our preliminary data demonstrate that HSV infection protects infected LCL or Jurkat cells from apoptosis triggered through the fas receptor...
  19. ROLE OF PHOSPHOLIPASE C IN T CELL ACTIVATION
    ROBERT GOLDFIEN; Fiscal Year: 1991
    ..T cell line, Jurkat expresses at least 2 isoforms of PI-PLC: PLCalpha and PLCgamma, and that stimulation of Jurkat cells with phytohemagglutinin and phorbol myristate acetate, leads to differential regulation of transcripts encoding ..
  20. ANKYRIN: CRUCIAL LINKER IN RENAL CELL APOPTOTIC CASCADES
    Prasad Devarajan; Fiscal Year: 2003
    ..apoptotic cells; (d) transfection of kidney ankyrin cDNA induces apoptosis in renal cell lines, as well as in Jurkat cells; and (e) the death domain of kidney ankyrin interacts with Fas...
  21. T Cells in the Pathogenesis of Systemic Sclerosis
    Chris Platsoucas; Fiscal Year: 2005
    ..a. To express the clonally expanded in SSc skin-biopsies TCR transcripts into ap TCR-negative J.RT3T3.5 Jurkat cells by transfection, or into normal CTL by infection with retroviral vectors. b...
  22. LENTIVIRUS TRANSDUCED DENDRITIC CELLS FOR HIV VACCINES
    June Kan Mitchell; Fiscal Year: 1999
    ..Specificity of the CTL will be measured by lysis of virus-infected A2.1-Jurkat cells. Reactivities to known A2-1-restricted epitopes of gag and pol also will be assessed with A2...
  23. p56lck Inhibitors as Potential Immunosuppressive Drugs.
    ALEXANDER MACKERELL; Fiscal Year: 2004
    ..screening for compounds that inhibit OKT-3 mediated stimulation of cellular protein tyrosine phosphorylation in Jurkat cells. 2.ii) Qualitative and quantitative p56lck SH2 binding analysis of active compounds from 2.i). 2...
  24. LYMPHOCYTE ADHESION AND HIV ACTIVATION IN THE LUNG
    LYNN SCHNAPP; Fiscal Year: 2000
    ..The ability of adhesion to affect transcription will be tested by examining Jurkat cells transfected with HIV LTR promoter upstream from the luciferase reporter gene...
  25. Quantitative Analysis of Cellular Signaling in Viral Latency
    Kathryn Miller Jensen; Fiscal Year: 2010
    ..Schaffer (sponsor) and Arkin (co-sponsor) showed that clonal populations of infected Jurkat cells with a single viral integration can either rapidly initiate viral gene expression or exhibit long periods of ..
  26. Analysis of Retinoid Mediated Anti Cancer Activities
    F Piedrafita; Fiscal Year: 2005
    ..protein synthesis and/or mRNA transcription are not necessary for the RRM-mediated activation of caspases in Jurkat cells, suggesting a novel mechanism of action for this particular class of apoptotic retinoids...
  27. Chromatin Dynamics in a Model of Latent HIV Infection
    Steven E Kauder; Fiscal Year: 2010
    ..Our laboratory has developed the J-Lat cell lines, a model system for the study of HIV latency in vitro. Jurkat cells were infected with recombinant HIV that expresses green fluorescence protein (GFP), and GFP fluorescence was ..
  28. NAK, A POTENTIAL THERAPEUTIC TARGET FOR AIDS
    Arie Abo; Fiscal Year: 2000
    ..To identify NAK, we will purify NAK from T Jurkat cells stably expressing Nef and will use the sequence information to obtain the full length DNA...
  29. Global Analysis of Proteolysis in the Innate Immune Response
    Nicholas J Agard; Fiscal Year: 2010
    ..Preliminary studies in apoptotic Jurkat cells have already identified 183 aspartate cleaved proteins, of which 151 are unreported caspase substrates...
  30. A New In Vitro Model for HIV Latency
    Eric Verdin; Fiscal Year: 2004
    ..HIV genome containing the reporter GFP (either in lieu of Nef or as a fusion with the env protein) in Jurkat cells using FACS. We will explore the role of different pathways for HIV latency activation...
  31. IDENTIFICATION OF A G PROTEIN IN PI PATHWAY IN T CELLS
    MARTHA GRABER; Fiscal Year: 1992
    ..Phase I The polymerase chain reaction will be used to obtain a series of G protein sequences in Jurkat cells. One or more sequences will be selected as candidates for the G protein which is involved in the PI pathway...
  32. MECHANISMS OF HIV1 TAT TRANSACTIVATION
    David Price; Fiscal Year: 2001
    ..These will include a detailed optimization of Tat transactivation in vitro using extracts from both HeLa and Jurkat cells. The effect of Tat on the association of P-TEFb, factor 2, the SPT4/SPT5 complex, and other factors with the ..
  33. MODULATION OF T CELL ACTIVITY BY 2-ARACHIDONYL GLYCEROL
    CHERYL ROCKWELL; Fiscal Year: 2004
    ..2-AG was not reversed by CB1/CB2 antagonists; b) 2-AG inhibited IL-2 transcription in HPB-ALL cells, but not in Jurkat cells, which express an aberrant CB2 receptor; and c) paradoxically, 2-AG also enhances T cell proliferation...
  34. INVESTIGATION OF SAFER TEST TUBES FOR LABORATORY USE
    HABIB SIOUFI; Fiscal Year: 1991
    ..Blood samples will be cultured in Supti and Jurkat cells (continuous T cell lines with a high expression of CD4) for 30 days...
  35. STRUCTURE/FUNCTION OF THE INTEGRIN ALPHA 1 BETA 1
    EUGENE MARCANTONIO; Fiscal Year: 2002
    ..The third aim will focus on a1b1 growth signals through the adaptor Shc. Studies in Jurkat cells found that the receptor tyrosine phosphatase CD45 and the Src kinase Lck are required and the Src kinase Fyn is ..
  36. Capricious Activation of T cells by Ethanol: A Possible Mechanism for Immunosuppr
    Martin Poenie; Fiscal Year: 2006
    ..We have used Jurkat cells stimulated by superantigen coated Raji cells as a ifiodel system for T cell activation and synapse formation...
  37. IS AAA AN ANTIGEN-DRIVEN AUTOIMMUNE DISEASE? 'COLLABORAT
    Chris Platsoucas; Fiscal Year: 2002
    ..RT3-T3.5 Jurkat cells by transfection, or into normal cytotoxic T-lymphocyte (CTL) lines by infection with retroviral vectors. b...
  38. INTERFERON SIGNAL TRANSDUCTION IN T CELLS
    Andrew Larner; Fiscal Year: 2001
    ..of these signaling enzymes in the biological activities of IFNalpha, wild-type, CD45-, Lck- or ZAP-70-deficient Jurkat cells were examined, and the antiproliferative effects of IFNalpha were completely abrogated in cell lines which did ..
  39. NOVEL CD32 ISOFORMS MODULATE NK CELL FUNCTIONS
    PENELOPE MOREL; Fiscal Year: 2003
    ..In addition, using Jurkat cells transfected with two FcgammaRIIc isoforms we have defined the ligand binding specificity and signal ..
  40. MITOCHONDRIAL ROLE IN CASPASE PROCESSING AND ACTIVATION
    Roberta Gottlieb; Fiscal Year: 2003
    We have made a preliminary observation that caspase-3 present in mitochondria from Jurkat cells, and that within ten minutes that Fas ligation, it is processed to a p29 form lacking the pro-domain...
  41. E2F TRANSCRIPTION FACTOR AND TUMOR SUPPRESSION
    Srikumar Chellappan; Fiscal Year: 2007
    ..We had found that Fas stimulation of Jurkat cells leads to an inactivation of Rb by phosphorylation, mainly brought about by the p38/Hog 1 kinase; similarly, ..
  42. PHOSPHATIDYLSERINE-SPECIFIC RECEPTOR FOR APOPTOTIC CELLS
    Valerie Fadok; Fiscal Year: 2004
    ..Transfection of the gene into Jurkat cells, which do not express the gene normally, induced the ability to bind to and take up apoptotic cells: this was ..
  43. PATHOGENETIC EVALUATION OF AIDS ASSOCIATED LYMPHOMAS
    Surendra Sharma; Fiscal Year: 1993
    ..after transfecting a BCGF upstream regulatory region--CAT construct into constitutively Tat producing Hela or Jurkat cells. To test for the transforming activity, both the tat expression construct and the BCGF-12kD expression ..
  44. TRAIL induced apoptotic pathways
    Roya Khosravi Far; Fiscal Year: 2001
    ..In addition, we will utilize the Jurkat cells that are deficient in caspase-8 to define the role of caspase-8 in mediating c-FLIP function...
  45. Discovery of Small Molecule Inhibitors of XIAP
    Shaomeng Wang; Fiscal Year: 2005
    ..b) Investigation of the molecular mechanism of action using Jurkat cells transfected with XlAP and vector control for several most potent small molecule inhibitors of XIAP.
  46. PROTEIN(S) INVOLVED IN NEUROTRANSMISSION
    Jean Shih; Fiscal Year: 2001
    ..The tissue distribution of factor b will be studied by Northern analysis. Factor b polypeptide(s) will also be expressed in resting Jurkat cells to see if it can stimulate IL-2 production.
  47. Tumor-reactive IgG in the diagnosis of ovarian cancer
    Douglas Taylor; Fiscal Year: 2005
    ..Ultimately, antigenic proteins, linked with histologic type and stage of disease, will be combined into the development of protein arrays to screen for circulating reactive IgG as an early diagnostic test. ..
  48. HTS for agents that increase recognition of tumors by cytotoxic lymphocytes
    TIMOTHY HAGGERTY; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  49. Rapid Detection of Major Food-borne Pathogens
    Peixuan Zhu; Fiscal Year: 2003
    ..The instrument will be redesigned to be more compact and portable, and assays developed for use outside a laboratory setting. Tests will be performed on location where these foods are produced, transported and/or prepared. ..
  50. Free radicals detection in aging brain by fullerenes
    VLADIMIR DIDENKO; Fiscal Year: 2003
    ..At a later stage free radicals will be monitored using magnetic resonance tracer and MRI detection. ..
  51. LENTIVIRAL GENE TRANSFER INTO PANCREATIC BETA-CELLS
    Mark Segal; Fiscal Year: 2003
    ....
  52. DEGRADABLE SCAFFOLDS FOR GENETICALLY ENGINEERED GRAFTS
    Jennifer West; Fiscal Year: 2002
    ....
  53. Role of P80 pro-IL-16 in T Cell Malignancy
    Yujun Zhang; Fiscal Year: 2008
    ..This study will lead to a considerable insight of the role of p80 pro-IL-16 in the T cell cycle regulation and T cell malignancy and help to identify diagnostic and/or therapeutical targets for human T cell malignancies. ..
  54. Targeting Apoptosis by Chemical Design
    LOREN WALENSKY; Fiscal Year: 2007
    ..potency in mitochondrial cytochrome c release, a key step in apoptosis; and (d) apoptosis induction in cultured Jurkat cells. SAHBs have the dual potential to serve as biological tools to dissect apoptotic pathways, and as prototype ..
  55. RhoH GTPase in blood cell and cancer development
    Yi Gu; Fiscal Year: 2006
    ..Given the lineage-restricted expression of RhoH such an observation could have important therapeutic implication. ..
  56. Chemokine Receptor Chimeras by Synthetic Gene Library
    Michael Dustin; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  57. The role of CD44 isoforms in immunotherapy of melanoma
    ROBERT MCKALLIP; Fiscal Year: 2006
    ..Furthermore, he proposes to examine the potential use of the CD44hi cells in the treatment of melanoma. [unreadable] [unreadable]..
  58. Activation of inflammatory cells by flagellin
    STEVEN MIZEL; Fiscal Year: 2006
    ..Furthermore, the proposed studies should also provide insights into the factors that regulate TLR signaling and allow for complex patterns of host cell response. ..
  59. Role of Vav family proteins in cell signaling and cancer
    XOSE BUSTELO; Fiscal Year: 2009
    ..abstract_text> ..
  60. ACTIVATION OF CELL SUICIDE IN THE IMMUNE SYSTEM
    David Ucker; Fiscal Year: 2002
    ..abstract_text> ..
  61. Role of CRH in skin physiology and pathology
    Andrzej Slominski; Fiscal Year: 2004
    ..g., melanocytes and keratinocytes, and test the role of CRH/CRH-R1 axis in the regulation of human hair growth. ..
  62. Characteristics of a putative steroid membrane receptor
    Peter Thomas; Fiscal Year: 2010
    ..However, it is important to establish these points for the mPR. In addition, the studies should provide indications of important regulatory events that might occur in human cells. ..
  63. Gene expression in memory CD4+T cells.
    Henry Wong; Fiscal Year: 2006
    ..abstract_text> ..
  64. NIR Absorbing Nanoparticles For Cancer Therapy
    Jennifer West; Fiscal Year: 2008
    ..We propose to evaluate biodistribution, biocompatibility and tumor ablation efficacy of these NIR-absorbing nanoparticles. The optimal particle formulation will be further examined in a modelof medulloblastoma. ..
  65. Structure and function of antimicrobial peptides
    Ayyalusamy Ramamoorthy; Fiscal Year: 2008
    ..These peptides have therapeutic potential as antibiotics and have particular importance for cystic fibrosis patients. ..
  66. Development of Vaccine for Neisseria gonorrhoeae
    Peixuan Zhu; Fiscal Year: 2005
    ..The data from this project will provide fundamental information for understanding the role of this protein in N. gonorrhoeae and the possibility to develop a gonococcal vaccine. ..
  67. Safety and efficacy of rh-Lactoferrin in Diabetic Ulcers
    Jose Engelmayer; Fiscal Year: 2004
    ..Incidence of partial and complete wound closure will also be observed. Safety results and any efficacy trends will be used to select rhLF doses for the proposed Phase II trial. ..
  68. Dock and Lock: novel protein engineering
    Chien Hsing Chang; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  69. Novel Caspase Substrates for Live Cells
    Hui Cen; Fiscal Year: 2007
    ..These novel caspase substrates will greatly facilitate our understanding of apoptosis and its relation to various diseases and the development of future therapeutics for apoptosis related diseases. [unreadable] [unreadable] [unreadable]..
  70. Cellular Targets for Gallium Compounds in Lymphoma
    Christopher Chitambar; Fiscal Year: 2008
    ..Our studies will provide new information regarding: a) the impact of HFE mutations on the response of lymphoma to gallium, and, b) the mechanism of action of gallium at the mitochondrial level. ..
  71. COPPER/ALBUMIN REDOX-CYCLING IN PREECLAMPSIA
    Valerian Kagan; Fiscal Year: 2003
    ....
  72. Case/Cleveland Clinical Research Training Program (RMI)
    Richard Rudick; Fiscal Year: 2004
    ..It will host a national conference on this theme during 2007 to exchange "lessons learned" and evaluate how NIH-sponsored programs are measuring up to this strategic goal. ..
  73. Respiratory Immunity Against Agents of Bioterrorism
    STEVEN MIZEL; Fiscal Year: 2008
    ....