hct116 cells

Summary

Summary: Human COLORECTAL CARCINOMA cell line.

Top Publications

  1. pmc Genetic inactivation of AKT1, AKT2, and PDPK1 in human colorectal cancer cells clarifies their roles in tumor growth regulation
    Kajsa Ericson
    The Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 107:2598-603. 2010
  2. ncbi Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer
    Andrew J Wilson
    Department of Oncology, Albert Einstein Cancer Center, Montefiore Medical Center, Bronx, New York 10467, USA
    J Biol Chem 281:13548-58. 2006
  3. pmc p53-Responsive micrornas 192 and 215 are capable of inducing cell cycle arrest
    Christian J Braun
    Department of Molecular Oncology, Göttingen Center of Molecular Biosciences, University of Gottingen, Gottingen, Germany
    Cancer Res 68:10094-104. 2008
  4. ncbi Dual targeting of HSC70 and HSP72 inhibits HSP90 function and induces tumor-specific apoptosis
    Marissa V Powers
    Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, UK
    Cancer Cell 14:250-62. 2008
  5. ncbi The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer
    Simone Spaderna
    Department of Visceral Surgery, University of Freiburg, Freiburg, Germany
    Cancer Res 68:537-44. 2008
  6. ncbi KRAB zinc finger protein ZNF382 is a proapoptotic tumor suppressor that represses multiple oncogenes and is commonly silenced in multiple carcinomas
    Yingduan Cheng
    Department of Clinical Oncology, State Key Laboratory in Oncology in South China Cancer Epigenetics Laboratory, Sir YK Pao Center for Cancer, Hong Kong Cancer Institute, Chinese University of Hong Kong, Shatin, Hong Kong
    Cancer Res 70:6516-26. 2010
  7. ncbi Modulation of microRNA processing by p53
    Hiroshi I Suzuki
    Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 460:529-33. 2009
  8. ncbi EGF signalling pathway regulates colon cancer stem cell proliferation and apoptosis
    Y Feng
    Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University of Medicine, Shanghai, China
    Cell Prolif 45:413-9. 2012
  9. pmc p53-Dependent p21-mediated growth arrest pre-empts and protects HCT116 cells from PUMA-mediated apoptosis induced by EGCG
    Vijay S Thakur
    Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA
    Cancer Lett 296:225-32. 2010
  10. ncbi NRF2 blockade suppresses colon tumor angiogenesis by inhibiting hypoxia-induced activation of HIF-1α
    Tae Hyoung Kim
    College of Pharmacy, Yeungnam University, Gyeongsangbuk do, South Korea
    Cancer Res 71:2260-75. 2011

Detail Information

Publications305 found, 100 shown here

  1. pmc Genetic inactivation of AKT1, AKT2, and PDPK1 in human colorectal cancer cells clarifies their roles in tumor growth regulation
    Kajsa Ericson
    The Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 107:2598-603. 2010
    ..These findings show that the PI3K signaling pathway is wired differently in human cancer cells than in other cell types or organisms, which has important implications for the design and testing of drugs that target this pathway...
  2. ncbi Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer
    Andrew J Wilson
    Department of Oncology, Albert Einstein Cancer Center, Montefiore Medical Center, Bronx, New York 10467, USA
    J Biol Chem 281:13548-58. 2006
    ....
  3. pmc p53-Responsive micrornas 192 and 215 are capable of inducing cell cycle arrest
    Christian J Braun
    Department of Molecular Oncology, Göttingen Center of Molecular Biosciences, University of Gottingen, Gottingen, Germany
    Cancer Res 68:10094-104. 2008
    ..Hence, miR-192 and miR-215 can act as effectors as well as regulators of p53; they seem to suppress cancerogenesis through p21 accumulation and cell cycle arrest...
  4. ncbi Dual targeting of HSC70 and HSP72 inhibits HSP90 function and induces tumor-specific apoptosis
    Marissa V Powers
    Signal Transduction and Molecular Pharmacology Team, Cancer Research UK Centre for Cancer Therapeutics, The Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey SM2 5NG, UK
    Cancer Cell 14:250-62. 2008
    ..Importantly, simultaneous silencing of HSP70 isoforms in nontumorigenic cell lines does not result in comparable growth arrest or induction of apoptosis, indicating a potential therapeutic window...
  5. ncbi The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer
    Simone Spaderna
    Department of Visceral Surgery, University of Freiburg, Freiburg, Germany
    Cancer Res 68:537-44. 2008
    ..Thus, by linking EMT, loss of polarity, and metastasis, ZEB1 is a crucial promoter of malignant tumor progression...
  6. ncbi KRAB zinc finger protein ZNF382 is a proapoptotic tumor suppressor that represses multiple oncogenes and is commonly silenced in multiple carcinomas
    Yingduan Cheng
    Department of Clinical Oncology, State Key Laboratory in Oncology in South China Cancer Epigenetics Laboratory, Sir YK Pao Center for Cancer, Hong Kong Cancer Institute, Chinese University of Hong Kong, Shatin, Hong Kong
    Cancer Res 70:6516-26. 2010
    ..Our data show that ZNF382 is a functional tumor suppressor frequently methylated in multiple carcinomas...
  7. ncbi Modulation of microRNA processing by p53
    Hiroshi I Suzuki
    Department of Molecular Pathology, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nature 460:529-33. 2009
    ..In HCT116 cells and human diploid fibroblasts, p53 interacts with the Drosha processing complex through the association with ..
  8. ncbi EGF signalling pathway regulates colon cancer stem cell proliferation and apoptosis
    Y Feng
    Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University of Medicine, Shanghai, China
    Cell Prolif 45:413-9. 2012
    ..This study assesses whether EGF, bFGF and IGF signalling pathways are essential for colon CSC proliferation and self-renewal...
  9. pmc p53-Dependent p21-mediated growth arrest pre-empts and protects HCT116 cells from PUMA-mediated apoptosis induced by EGCG
    Vijay S Thakur
    Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA
    Cancer Lett 296:225-32. 2010
    ..p21, thus can be used as a molecular switch for therapeutic intervention of colon cancer...
  10. ncbi NRF2 blockade suppresses colon tumor angiogenesis by inhibiting hypoxia-induced activation of HIF-1α
    Tae Hyoung Kim
    College of Pharmacy, Yeungnam University, Gyeongsangbuk do, South Korea
    Cancer Res 71:2260-75. 2011
    ....
  11. ncbi Small molecule XIAP inhibitors enhance TRAIL-induced apoptosis and antitumor activity in preclinical models of pancreatic carcinoma
    Meike Vogler
    University Children s Hospital, Ulm University, Ulm, Germany
    Cancer Res 69:2425-34. 2009
    ..These findings build the rationale for further (pre)clinical development of XIAP inhibitors and TRAIL against pancreatic cancer...
  12. pmc Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
    ..The discovery also adds to the connection between replication initiation and sister chromatid cohesion...
  13. ncbi B-MYB is required for recovery from the DNA damage-induced G2 checkpoint in p53 mutant cells
    Mirijam Mannefeld
    Department of Physiological Chemistry I, Biocenter, University of Wurzburg, Wurzburg, Germany
    Cancer Res 69:4073-80. 2009
    ..Taken together, these data suggest that B-MYB/LINC plays an important role in the DNA damage response downstream of p53...
  14. ncbi Selenite induces redox-dependent Bax activation and apoptosis in colorectal cancer cells
    Fang Huang
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
    Free Radic Biol Med 46:1186-96. 2009
    ..Our results show for the first time that selenite can activate the apoptotic machinery through redox-dependent activation of Bax and further suggest that selenite could be useful in cancer therapy...
  15. pmc NOTCH signaling is required for formation and self-renewal of tumor-initiating cells and for repression of secretory cell differentiation in colon cancer
    Shaheen S Sikandar
    Department of Biological Chemistry, School of Medicine, University of California, Irvine, California, USA
    Cancer Res 70:1469-78. 2010
    ..Our findings describe a novel human cell system to study NOTCH signaling in CRC tumor initiation and suggest that inhibition of NOTCH signaling may improve CRC chemoprevention and chemotherapy...
  16. pmc The Bad guy cooperates with good cop p53: Bad is transcriptionally up-regulated by p53 and forms a Bad/p53 complex at the mitochondria to induce apoptosis
    Peng Jiang
    School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People s Republic of China
    Mol Cell Biol 26:9071-82. 2006
    ..Hence, our collective data provide the first evidence that Bad plays dual roles in both p53 transcription-dependent and -independent pathways...
  17. pmc MicroRNA-21 induces stemness by downregulating transforming growth factor beta receptor 2 (TGFβR2) in colon cancer cells
    Yingjie Yu
    Department of Veterans Affairs Medical Center, Detroit, MI 48201, USA
    Carcinogenesis 33:68-76. 2012
    ..Our current data suggest that miR-21 plays an important role in regulating stemness by modulating TGFβR2 signaling in colon cancer cells...
  18. ncbi SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events
    J Yu
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Oncogene 26:4189-98. 2007
    ....
  19. pmc Distinct activation pathways confer cyclin-binding specificity on Cdk1 and Cdk2 in human cells
    Karl A Merrick
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Mol Cell 32:662-72. 2008
    ..Therefore, differential ordering of common activation steps promotes CDK-cyclin specificity, with Cdk7 acting catalytically to enforce fidelity...
  20. ncbi Silenced tumor suppressor genes reactivated by DNA demethylation do not return to a fully euchromatic chromatin state
    Kelly M McGarvey
    Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, 1650 Orleans Street, Baltimore, MD 21231, USA
    Cancer Res 66:3541-9. 2006
    ..This finding has important implications for the translational goal of reactivating aberrantly silenced cancer genes as a therapeutic maneuver...
  21. ncbi Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells
    Rui Dong Li
    Department of Orthopaedic Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China
    BMB Rep 45:509-14. 2012
    ..Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells...
  22. pmc Disruption of the VDAC2-Bak interaction by Bcl-x(S) mediates efficient induction of apoptosis in melanoma cells
    M Plötz
    Department of Dermatology and Allergy, Skin Cancer Center Charite, Charite Universitatsmedizin Berlin, Chariteplatz 1, 10117 Berlin, Germany
    Cell Death Differ 19:1928-38. 2012
    ..Further supporting this pathway, overexpression of VDAC2 strongly decreased apoptosis by Bcl-x(S). New proapoptotic pathways are of principle interest for overcoming apoptosis deficiency of melanoma cells...
  23. pmc Functional analysis of p53 binding under differential stresses
    Adam J Krieg
    Division of Radiation and Cancer Biology, Department of Radiation Oncology, Stanford University, Stanford, CA 94303 5152, USA
    Mol Cell Biol 26:7030-45. 2006
    ..hybridization to identify promoters bound by p53 under both DNA-damaging and non-DNA-damaging conditions in HCT116 cells. Using gene-specific PCR analysis, we have verified an association with CGIs of the highest enrichment (> 2...
  24. pmc The impact of S- and G2-checkpoint response on the fidelity of G1-arrest by cisplatin and its comparison to a non-cross-resistant platinum(IV) analog
    Guangan He
    Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Gynecol Oncol 122:402-9. 2011
    ..Therefore, we investigated this paradox, and used the model analog DAP as a positive control for p21-dependent G1-arrest...
  25. pmc Hypoxia-inducible factor-2α activation promotes colorectal cancer progression by dysregulating iron homeostasis
    Xiang Xue
    Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA
    Cancer Res 72:2285-93. 2012
    ..Together, our findings show that a chronic increase in HIF-2α in the colon initiates protumorigenic signaling, which may have important implications in developing preventive and therapeutic strategies for colon cancer...
  26. pmc IL-32γ inhibits cancer cell growth through inactivation of NF-κB and STAT3 signals
    J H Oh
    College of Pharmacy and Medical Research Center, Chungbuk National University, Chungbuk, Korea
    Oncogene 30:3345-59. 2011
    ..However, siRNA of NF-κB and STAT3 augmented IL-32γ-induced colon cancer cell growth inhibition. These findings indicate significant pathophysiological roles of IL-32γ in cancer development...
  27. ncbi Flavokawain B, a novel chalcone from Alpinia pricei Hayata with potent apoptotic activity: Involvement of ROS and GADD153 upstream of mitochondria-dependent apoptosis in HCT116 cells
    Yu Feng Kuo
    Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung 40227, Taiwan, Republic of China
    Free Radic Biol Med 49:214-26. 2010
    ..The up-regulation of GADD153 in flavokawain B-treated HCT116 cells is associated with mitochondrial dysfunction and altered expression of Bcl-2 family members...
  28. ncbi Potentiation of chemotherapeutic drugs by energy metabolism inhibitors 2-deoxyglucose and etomoxir
    Emma Hernlund
    Department of Oncology Pathology, Karolinska Institute, S 171 76 Stockholm, Sweden
    Int J Cancer 123:476-83. 2008
    ..We also conclude that although the potentiated drugs as yet have no obvious mechanistic factor in common, the strategy holds promise with genotoxic as well non-genotoxic anticancer drugs...
  29. ncbi MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells
    Pedro M Borralho
    Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
    FEBS J 276:6689-700. 2009
    ..Collectively, the data obtained in the present study suggest anti-proliferative, chemosensitizer and putative pro-apoptotic roles for miR-143 in colon cancer...
  30. pmc Predominant requirement of Bax for apoptosis in HCT116 cells is determined by Mcl-1's inhibitory effect on Bak
    C Wang
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Oncogene 31:3177-89. 2012
    ..Our data suggest that Bax and Bak are functionally redundant, but they are counteracted by distinct anti-apoptotic Bcl-2 family proteins in different species...
  31. ncbi Selection of cancer cells with repressed mitochondria triggers colon cancer progression
    María Sánchez-Aragó
    Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas Universidad Autónoma de Madrid CSIC UAM and Centro de Investigación Biomédica en Red de Enfermedades Raras CIBERER ISCIII, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Carcinogenesis 31:567-76. 2010
    ..Treatment of cancer cells with dichloroacetate partially restores the functional differentiation of mitochondria and promotes tumor regression, emphasizing the reversible nature of the metabolic trait of cancer...
  32. pmc bis-Dehydroxy-Curcumin triggers mitochondrial-associated cell death in human colon cancer cells through ER-stress induced autophagy
    Valentina Basile
    Dipartimento di Scienze della Vita, Universita di Modena e Reggio Emilia, Via Campi 213 D, Modena, Italy
    PLoS ONE 8:e53664. 2013
    ..A complex interplay between autophagy and apoptosis has come to light, taking into account that numerous genes, such as p53 and Bcl-2 family members, are shared between these two pathways...
  33. ncbi RHAMM is differentially expressed in the cell cycle and downregulated by the tumor suppressor p53
    Sindy Sohr
    Molecular Oncology, Department of Obstetrics and Gynecology, University of Leipzig, Leipzig, Germany
    Cell Cycle 7:3448-60. 2008
    ..Additionally, p53-dependent downregulation is consistent with an oncogenic function of RHAMM and the recently reported tumor-suppressive function of CD44 transcriptional repression by p53...
  34. ncbi Selenite-induced apoptosis and autophagy in colon cancer cells
    Vera Králová
    Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Simkova 870, 500 38 Hradec Kralove, Czech Republic
    Toxicol In Vitro 26:258-68. 2012
    ....
  35. ncbi Ezrin is a specific and direct target of protein tyrosine phosphatase PRL-3
    Eleonora Forte
    IRBM P Angeletti, Via Pontina, Pomezia, Rome, Italy
    Biochim Biophys Acta 1783:334-44. 2008
    ..Although PRL-3 over-expression in HCT116 cells appeared to affect Ezrin phosphorylation status at both tyrosine residues and Thr567, suppression of the ..
  36. pmc Ubiquitination of mammalian AP endonuclease (APE1) regulated by the p53-MDM2 signaling pathway
    C S Busso
    Department of Otolaryngology, Stanley S Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Oncogene 28:1616-25. 2009
    ..Therefore, monoubiquitination not only is a prerequisite for degradation, but may also alter the APE1 activities in cells. These results reveal a novel regulation of APE1 through ubiquitination...
  37. ncbi Inhibition of NEDD8-activating enzyme induces rereplication and apoptosis in human tumor cells consistent with deregulating CDT1 turnover
    Michael A Milhollen
    Discovery, Millennium Pharmaceuticals, Inc, Cambridge, Massachusetts 02139, USA
    Cancer Res 71:3042-51. 2011
    ..DNA rereplication is an unrecoverable cellular insult and the small molecule inhibitor MLN4924, currently in phase I trials, represents an unprecedented opportunity to explore this mechanism of cytotoxicity for the treatment of cancer...
  38. pmc Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells
    Farjana J Fattah
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 105:8703-8. 2008
    ..Our data suggest that gene-targeting frequencies can be significantly improved in human cells by impairing the NHEJ pathway, and we propose that Ku70 depletion can be used to facilitate both knockout and gene therapy approaches...
  39. ncbi Induction of senescence with doxorubicin leads to increased genomic instability of HCT116 cells
    Malgorzata A Sliwinska
    Department of Biochemistry, Nencki Institute of Experimental Biology, Pasteura 3, 02 093 Warsaw, Poland
    Mech Ageing Dev 130:24-32. 2009
    ..We showed that after treatment of HCT116 cells with a low dose of doxorubicin most of them stopped proliferation as documented by SA-beta-galactosidase ..
  40. ncbi Regulation of gamma-H2AX and securin contribute to apoptosis by oxaliplatin via a p38 mitogen-activated protein kinase-dependent pathway in human colorectal cancer cells
    Shu Jun Chiu
    Department of Life Science, Tzu Chi University, Hualien 970, Taiwan
    Toxicol Lett 179:63-70. 2008
    ..Our findings suggest that p38 MAPK may oppositely regulate securin protein expression and gamma-H2AX formation in the oxaliplatin-induced apoptosis of human colorectal cancer cells...
  41. pmc Translational repression of p53 by RNPC1, a p53 target overexpressed in lymphomas
    Jin Zhang
    Comparative Cancer Center, Schools of Medicine and Veterinary Medicine, University of California at Davis, USA
    Genes Dev 25:1528-43. 2011
    ..Together, we identified a novel p53-RNPC1 autoregulatory loop, and our findings suggest that RNPC1 plays a role in tumorigenesis by repressing p53 translation...
  42. ncbi Mapping of a candidate colorectal cancer tumor-suppressor gene to a 900-kilobase region on the short arm of chromosome 8
    James M Flanagan
    The Queensland Institute of Medical Research, Royal Brisbane Hospital, Herston, Queensland, Australia
    Genes Chromosomes Cancer 40:247-60. 2004
    ..Despite the rarity of somatic mutations, the expression data suggest that KIAA1456 is still a candidate for the putative 8p colorectal cancer tumor-suppressor gene...
  43. ncbi Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage
    Amanda B Hummon
    National Cancer Institute, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA
    Biotechniques 42:467-70, 472. 2007
    ....
  44. pmc Synthetic lethality of cohesins with PARPs and replication fork mediators
    Jessica L McLellan
    Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    PLoS Genet 8:e1002574. 2012
    ....
  45. ncbi Securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis
    Sz Hsien Yu
    Department of Life Science, Tzu Chi University, Hualien, Taiwan
    Cancer Lett 300:96-104. 2011
    ..Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay, Annexin V-FITC/PI double staining, Ser15-phosphorylation of p53, and cleavages of ..
  46. pmc A small molecule Inauhzin inhibits SIRT1 activity and suppresses tumour growth through activation of p53
    Qi Zhang
    Department of Biochemistry and Molecular Biology and Cancer Center, Tulane University School of Medicine, Louisiana, LA, USA
    EMBO Mol Med 4:298-312. 2012
    ..tumour-specific apoptosis, and represses the growth of xenograft tumours derived from p53-harbouring H460 and HCT116 cells without causing apparent toxicity to normal tissues and the tumour-bearing SCID mice...
  47. pmc The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions
    Zhigang Yuan
    H Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA
    Mol Cell Biol 30:3004-15. 2010
    ..Together, these results provide novel mechanistic insights into the function of ATDC and offer an explanation for how ATDC promotes cancer cell proliferation...
  48. pmc The mismatch repair system modulates curcumin sensitivity through induction of DNA strand breaks and activation of G2-M checkpoint
    Zhihua Jiang
    Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 9:558-68. 2010
    ..The results have potential therapeutic value, especially in the treatment of tumors with compromised MMR function...
  49. ncbi Antiangiogenic activities and cisplatin-combined antitumor activities of BPR0L075
    Ching Ping Chen
    Division of Biotechnology and Pharmaceutical Research, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli 35053, Taiwan, R O C
    Anticancer Res 30:2813-22. 2010
    ..Antimitotic tubulin-binding BPR0L075 is structurally analogous to the vascular-disrupting combretastatin A-4...
  50. pmc Mitochondrial respiration protects against oxygen-associated DNA damage
    Ho Joong Sung
    Translational Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Nat Commun 1:5. 2010
    ..Our study highlights the importance of mitochondrial respiration both for bioenergetic benefits and for maintaining genomic stability in an oxygen-rich environment...
  51. pmc Independent interactions of phosphorylated β-catenin with E-cadherin at cell-cell contacts and APC at cell protrusions
    Maree C Faux
    Ludwig Institute for Cancer Research, Melbourne, Victoria, Australia
    PLoS ONE 5:e14127. 2010
    ..The APC tumour suppressor functions in several cellular processes including the regulation of β-catenin in Wnt signalling and in cell adhesion and migration...
  52. ncbi Depletion of securin induces senescence after irradiation and enhances radiosensitivity in human cancer cells regardless of functional p53 expression
    Wen Shu Chen
    Department of Life Science, Tzu Chi University, Hualien, Taiwan
    Int J Radiat Oncol Biol Phys 77:566-74. 2010
    ..The development of new strategies to trigger cancer cell death besides apoptosis is necessary. This study investigated the role of securin in radiation-induced apoptosis and senescence in human cancer cells...
  53. pmc miR-192 Regulates dihydrofolate reductase and cellular proliferation through the p53-microRNA circuit
    Bo Song
    Translational Research Laboratory, Department of Pathology, Stony Brook University Medical Center, Stony Brook, New York 11794 8691, USA
    Clin Cancer Res 14:8080-6. 2008
    ..The purpose of this study is to investigate the molecular mechanism of miR-192 in colon cancer...
  54. ncbi Regulatory mechanisms controlling human E-cadherin gene expression
    Yan Nan Liu
    Graduate Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan
    Oncogene 24:8277-90. 2005
    ..These findings suggested HNF3 plays important roles in the upregulation of the E-cadherin gene and may be able to reduce the motility of metastatic breast cancer cells...
  55. ncbi E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly
    Prajakta Gosavi
    Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar Node, Navi Mumbai, India
    Cell Mol Life Sci 68:1439-54. 2011
    ..The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to initiate desmosome formation...
  56. ncbi Growth response of human colorectal tumour cell lines to treatment with afatinib (BIBW2992), an irreversible erbB family blocker, and its association with expression of HER family members
    Said Abdullah Khelwatty
    School of Life Sciences, Kingston University London, Kingston, Surrey, UK
    Int J Oncol 39:483-91. 2011
    ..This approach could lead to the identification of a specific subpopulation of cancer patients more likely to benefit from erbB-directed therapy...
  57. pmc Oxidative damage targets complexes containing DNA methyltransferases, SIRT1, and polycomb members to promoter CpG Islands
    Heather M O'Hagan
    Department of Oncology and The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Cancer Cell 20:606-19. 2011
    ..Thus, oxidative damage induces formation and relocalization of a silencing complex that may explain cancer-specific aberrant DNA methylation and transcriptional silencing...
  58. pmc Absence of p300 induces cellular phenotypic changes characteristic of epithelial to mesenchyme transition
    D Krubasik
    Department of Oncology, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Br J Cancer 94:1326-32. 2006
    ..Comparison of epithelial and mesenchymal proteins in p300- with parental HCT116 cells showed that a number of genes involved in cell and extracellular matrix interactions, typical of 'epithelial to ..
  59. pmc DNA damage response to the Mdm2 inhibitor nutlin-3
    Rajeev Verma
    Department of Molecular and Cell Biology U3125, University of Connecticut, Storrs, CT 06269, USA
    Biochem Pharmacol 79:565-74. 2010
    ..Finally, p21 deletion also sensitized HCT116 cells to the Nutlin-3-induced DNA damage response, suggesting that cell cycle checkpoint abnormalities may promote ..
  60. pmc Trop2 expression contributes to tumor pathogenesis by activating the ERK MAPK pathway
    Rafael Cubas
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cancer 9:253. 2010
    ..Here, we studied the effects murine Trop2 (mTrop2) exerted on tumor cellular functions and some of the signaling mechanisms activated by this oncogene...
  61. pmc PUMA induction by FoxO3a mediates the anticancer activities of the broad-range kinase inhibitor UCN-01
    Crissy Dudgeon
    Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 9:2893-902. 2010
    ....
  62. ncbi p53 suppresses the Nrf2-dependent transcription of antioxidant response genes
    Raffaella Faraonio
    Dipartimento di Biochimica e Biotecnologie Mediche, Universita di Napoli Federico II, CEINGE Biotecnologie Avanzate, 80131 Napoli, Italy
    J Biol Chem 281:39776-84. 2006
    ....
  63. pmc New Topoisomerase I mutations are associated with resistance to camptothecin
    Celine Gongora
    Institut de Recherche en Cancérologie de Montpellier, INSERM, Université Montpellier1, CRLC Val d Aurelle Paul Lamarque, Montpellier, France
    Mol Cancer 10:64. 2011
    ..Irinotecan is widely used as an anti-cancer agent in the treatment of metastatic colon cancer. However, its efficacy is often limited by the development of resistance...
  64. pmc The catalytic subunit of DNA-dependent protein kinase regulates proliferation, telomere length, and genomic stability in human somatic cells
    Brian L Ruis
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Cell Biol 28:6182-95. 2008
    ..Surprisingly, DNA-PK(cs) does not appear to be essential, although the cell line lacking this gene has profound proliferation and genomic stability deficits not observed for other mammalian systems...
  65. ncbi In vivo biological activity of the histone deacetylase inhibitor LAQ824 is detectable with 3'-deoxy-3'-[18F]fluorothymidine positron emission tomography
    Julius Leyton
    Molecular Therapy and Cancer Cell Biology, Imperial College London, Faculty of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Cancer Res 66:7621-9. 2006
    ..Drug-induced changes in tumor [18F]FLT uptake were due, at least in part, to reductions in TK1 transcription and translation...
  66. ncbi Evaluation of the proteasome inhibitor MLN9708 in preclinical models of human cancer
    Erik Kupperman
    Millennium Pharmaceuticals, Inc, Cambridge, Massachusetts 02139, USA
    Cancer Res 70:1970-80. 2010
    ..Moreover, antitumor activity was shown via multiple dosing routes, including oral gavage. Taken together, these data support the clinical development of MLN9708 for both hematologic and solid tumor indications...
  67. pmc Cellular stress induced alterations in microRNA let-7a and let-7b expression are dependent on p53
    Anthony D Saleh
    Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS ONE 6:e24429. 2011
    ....
  68. ncbi Involvement of RNA helicases p68 and p72 in colon cancer
    Sook Shin
    Department of Biochemistry, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Cancer Res 67:7572-8. 2007
    ..Altogether, these results suggest that p68/p72 overexpression is not only a potential marker of colon cancer but is also causally linked to this disease. Therefore, p68 and p72 may be novel targets in the combat against colon cancer...
  69. ncbi Antitumor efficacy of PKI-587, a highly potent dual PI3K/mTOR kinase inhibitor
    Robert Mallon
    Department of Oncology, Discovery Medicinal Chemistry, and Drug Safety and Metabolism, Wyeth Research now Pfizer, Pearl River, New York 10965, USA
    Clin Cancer Res 17:3193-203. 2011
    ..The aim of this study was to show preclinical efficacy and clinical development potential of PKI-587, a dual phosphoinositide 3-kinase (PI3K)/mTOR inhibitor...
  70. ncbi Cell cycle dependent and schedule-dependent antitumor effects of sorafenib combined with radiation
    John P Plastaras
    Laboratory of Molecular Oncology and Cell Cycle Regulation, Department of Medicine Hematology Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 67:9443-54. 2007
    ..This study establishes a foundation for clinical testing of sequential fractionated radiation followed by sorafenib in gastrointestinal and other malignancies...
  71. pmc Curcumin inhibits the proteasome activity in human colon cancer cells in vitro and in vivo
    Vesna Milacic
    Barbara Ann Karmanos Cancer Institute, Department of Pathology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    Cancer Res 68:7283-92. 2008
    ....
  72. ncbi Synergistic antitumor activity of sorafenib in combination with epidermal growth factor receptor inhibitors in colorectal and lung cancer cells
    Erika Martinelli
    Dipartimento Medico Chirurgico di Internistica Clinica e Sperimentale F Magrassi e A Lanzara, Seconda Universita degli Studi di Napoli, Napoli, Italy
    Clin Cancer Res 16:4990-5001. 2010
    ..Cancer cell survival, invasion, and metastasis depend on cancer cell proliferation and on tumor-induced angiogenesis. We evaluated the efficacy of the combination of sorafenib and erlotinib or cetuximab...
  73. ncbi Suppression of mitochondrial NADP(+)-dependent isocitrate dehydrogenase activity enhances curcumin-induced apoptosis in HCT116 cells
    Kyu Ho Jung
    School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu 702 701, Korea
    Free Radic Res 45:431-8. 2011
    ..Transfection of HCT116 cells with an IDPm small interfering RNA (siRNA) markedly decreased activity of IDPm, enhancing cellular ..
  74. ncbi Chromatin immunoprecipitation-on-chip reveals stress-dependent p53 occupancy in primary normal cells but not in established cell lines
    Helena Shaked
    Department of Molecular Biology, Microarray Service Laboratory, The Core Research Facility, and Lautenberg Center for General and Tumor Immunology, Hebrew University Medical School, Jerusalem, Israel
    Cancer Res 68:9671-7. 2008
    ..Our results suggest that nonactivated p53 has limited binding activity, whereas upon activation it binds to essentially all its targets. Additional triggers are most likely required to activate the transcriptional program of p53...
  75. pmc Mcl-1 determines the Bax dependency of Nbk/Bik-induced apoptosis
    Bernhard Gillissen
    Department of Hematology, Oncology, and Tumor Immunology, University Medical Center Charite, 13125 Berlin, Germany
    J Cell Biol 179:701-15. 2007
    ..The finding that different BH3-only proteins rely specifically on Bax, Bak, or both has important implications for the design of anticancer drugs targeting Bcl-2...
  76. pmc Differential control of TAp73 and DeltaNp73 protein stability by the ring finger ubiquitin ligase PIR2
    Berna S Sayan
    Medical Research Council, Toxicology Unit, Leicester University, Leicester LE1 9HN, United Kingdom
    Proc Natl Acad Sci U S A 107:12877-82. 2010
    ..These results suggest that PIR2, by being induced by TAp73 and degrading DeltaNp73, differentially regulates TAp73/DeltaNp73 stability, and, hence, it may offer a therapeutic approach to enhance the chemosensitivity of tumor cells...
  77. pmc Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway
    Margareta T Wilhelm
    The Campbell Family Institute for Breast Cancer Research, Princess Margaret Hospital, Toronto, Ontario, Canada
    Genes Dev 24:549-60. 2010
    ..This novel finding may explain why human tumors with high levels of DeltaNp73 expression show enhanced resistance to chemotherapy...
  78. pmc Selective blockade of DCAMKL-1 results in tumor growth arrest by a Let-7a MicroRNA-dependent mechanism
    Sripathi M Sureban
    Department of Medicine, Digestive Diseases and Nutrition Section, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    Gastroenterology 137:649-59, 659.e1-2. 2009
    ..Microtubule-associated kinase DCAMKL-1 is a putative intestinal stem cell marker that is expressed in Apc(Min/+) adenomas. We investigated the role of DCAMKL-1 on expression of let-7a miRNA and the oncogene c-Myc and in tumorigenesis...
  79. pmc Ku86 represses lethal telomere deletion events in human somatic cells
    Yongbao Wang
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 106:12430-5. 2009
    ..Thus, Ku86 is an essential gene in human somatic cells because of its requirement, not in NHEJ or V(D)J recombination, but in telomere maintenance...
  80. ncbi Characterization of HCT116 human colon cancer cells in an orthotopic model
    Ashwani Rajput
    Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    J Surg Res 147:276-81. 2008
    ..The purpose of this study was to characterize the behavior of a human colon cancer cell line, HCT116 in an orthotopic model...
  81. ncbi Cancer-specific functions of SIRT1 enable human epithelial cancer cell growth and survival
    Jack Ford
    YCR P53 Research Laboratory, Department of Biology, University of York, York, United Kingdom
    Cancer Res 65:10457-63. 2005
    ..We further identify caspase-3 and caspase-7 as downstream executioners of SIRT1/FoxO4-regulated apoptosis. Our work identifies SIRT1 as a novel target for selective killing of cancer versus noncancer epithelial cells...
  82. pmc PIK3CA mutation/PTEN expression status predicts response of colon cancer cells to the epidermal growth factor receptor inhibitor cetuximab
    Minaxi Jhawer
    Department of Oncology, Montefiore Medical Center, Albert Einstein Cancer Center, Bronx, NY 10467, USA
    Cancer Res 68:1953-61. 2008
    ..0% versus 38.5 +/- 6.4% growth inhibition, mean +/- SE; P = 0.008). Consistently, PIK3CA mutant isogenic HCT116 cells showed increased resistance to cetuximab compared with PIK3CA WT controls...
  83. pmc A frequent kinase domain mutation that changes the interaction between PI3Kalpha and the membrane
    Diana Mandelker
    The Ludwig Center for Cancer Genetics and Therapeutics and Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 106:16996-7001. 2009
    ..These structural and biochemical data suggest a previously undescribed mechanism for mutational activation of a kinase that involves perturbation of its interaction with the cellular membrane...
  84. ncbi Genistein, a dietary isoflavone, down-regulates the MDM2 oncogene at both transcriptional and posttranslational levels
    Mao Li
    Department of Pharmacology and Toxicology, Division of Clinical Pharmacology, University of Alabama at Birmingham, 35294, USA
    Cancer Res 65:8200-8. 2005
    ..In conclusion, genistein directly down-regulates the MDM2 oncogene, representing a novel mechanism of its action that may have implications for its chemopreventive and chemotherapeutic effects...
  85. ncbi The p53 pathway promotes efficient mitochondrial DNA base excision repair in colorectal cancer cells
    Dexi Chen
    Department of Medicine, Division of Hematology and Medical Oncology, Oregon Health and Science University, Portland, OR 97201, USA
    Cancer Res 66:3485-94. 2006
    ..Our data suggest that an intact p53 pathway stimulates specific mtBER steps and provides mechanistic insight into the development of mtDNA mutations in colorectal cancer...
  86. pmc Hypoxia-selective macroautophagy and cell survival signaled by autocrine PDGFR activity
    Simon Wilkinson
    Tumour Cell Death Laboratory, Beatson Institute for Cancer Research, Glasgow, United Kingdom
    Genes Dev 23:1283-8. 2009
    ..As autocrine growth factor signaling is a hallmark of many cancers, cell-autonomous enhancement of HIF1alpha-mediated macroautophagy may represent a mechanism for augmenting tumor cell survival under hypoxic conditions...
  87. pmc DACT3 is an epigenetic regulator of Wnt/beta-catenin signaling in colorectal cancer and is a therapeutic target of histone modifications
    Xia Jiang
    Cancer Biology and Pharmacology, Genome Institute of Singapore, A STAR Agency for Science, Technology and Research, Biopolis, 138672 Singapore
    Cancer Cell 13:529-41. 2008
    ..Our study identifies DACT3 as an important regulator of Wnt/beta-catenin signaling in colorectal cancer and suggests a potential strategy for therapeutic control of Wnt/beta-catenin signaling in colorectal cancer...
  88. ncbi Identification of Trop-2 as an oncogene and an attractive therapeutic target in colon cancers
    Jianbo Wang
    Division of Oncology, Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA
    Mol Cancer Ther 7:280-5. 2008
    ..Therefore, we have identified Trop-2 as an oncogene that has potential as a therapeutic target. Given the restricted expression of Trop-2 in normal tissue, anti-Trop-2 therapeutics would be predicted to have limited toxicity...
  89. pmc VAMP8/endobrevin is overexpressed in hyperreactive human platelets: suggested role for platelet microRNA
    A A Kondkar
    Thomas Jefferson University, The Cardeza Foundation for Hematologic Research and the Department of Medicine, Jefferson Medical College, Philadelphia, PA 19107, USA
    J Thromb Haemost 8:369-78. 2010
    ..Variation in platelet reactivity contributes to disorders of hemostasis and thrombosis, but the molecular mechanisms are not well understood...
  90. pmc Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma
    Seong Hwan Park
    Laboratory of Systems Mucosal Biomodulation, Department of Microbiology and Immunology, Pusan National University School of Medicine, Yangsan 626 813, Korea
    J Biol Chem 285:35330-9. 2010
    ....
  91. pmc TGF-beta1-induced expression of human Mdm2 correlates with late-stage metastatic breast cancer
    Shinako Araki
    Department of Oncology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd, Dallas, Texas 75390 8807, USA
    J Clin Invest 120:290-302. 2010
    ..Identification of Hdm2 as a downstream target of TGF-beta1 represents a critical prosurvival mechanism in cancer progression and provides another point for therapeutic intervention in late-stage cancer...
  92. ncbi Selenite induces apoptosis in colorectal cancer cells via AKT-mediated inhibition of β-catenin survival axis
    Hui Luo
    National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
    Cancer Lett 315:78-85. 2012
    ....
  93. pmc Silibinin suppresses growth of human colorectal carcinoma SW480 cells in culture and xenograft through down-regulation of beta-catenin-dependent signaling
    Manjinder Kaur
    Department of Pharmaceutical Sciences, School of Pharmacy, Aurora, CO, USA
    Neoplasia 12:415-24. 2010
    ..growth, induced cell death, and decreased nuclear and cytoplasmic levels of beta-catenin in SW480 but not in HCT116 cells, suggesting its selective effect on the beta-catenin pathway and associated biologic responses...
  94. pmc A Cdk7-Cdk4 T-loop phosphorylation cascade promotes G1 progression
    Miriam Merzel Schachter
    Department of Structural and Chemical Biology, Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029 6574, USA
    Mol Cell 50:250-60. 2013
    ..Therefore, mitogen signaling drives a CDK-activation cascade during G1 progression, and CAK might be rate-limiting for R point passage...
  95. pmc Ras puts the brake on doxorubicin-mediated cell death in p53-expressing cells
    Sunil K Manna
    Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics, Nampally, Hyderabad 500 001, India
    J Biol Chem 286:7339-47. 2011
    ..High basal expression of Fas might be an important determinant in doxorubicin-mediated cell death in p53-negative cells...
  96. ncbi Asian ginseng enhances the anti-proliferative effect of 5-fluorouracil on human colorectal cancer: comparison between white and red ginseng
    Anna B Fishbein
    Tang Center for Herbal Medicine Research, University of Chicago, Chicago, IL, 60637, USA
    Arch Pharm Res 32:505-13. 2009
    ..Rd and Rg3 showed positive anti-proliferative effect. Our data suggested a potential for RG as an adjuvant therapy in the treatment of colorectal cancer, via a synergistic action...
  97. pmc Cross-talk between phospho-STAT3 and PLCγ1 plays a critical role in colorectal tumorigenesis
    Peng Zhang
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106 7285, USA
    Mol Cancer Res 9:1418-28. 2011
    ..In aggregate, our study identifies previously unknown cross-talk between STAT3 and the PLCγ signaling pathways that may play a critical role in colorectal tumorigenesis...
  98. pmc Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53
    Binghui Li
    Ben May Department for Cancer Research, The University of Chicago, IL 60637, United States
    Cancer Lett 301:185-92. 2011
    ..Blockage of ROS by NAC or catalase inhibited the activation of NF-κB signaling and enhanced Rh2-induced cell death, suggesting that the anti-cancer effect of Rh2 can be enhanced by antioxidants...
  99. ncbi Molecular effects of baicalein in Hct116 cells and Caenorhabditis elegans : activation of the Nrf2 signaling pathway and prolongation of lifespan
    Susannah Havermann
    Institute of Toxicology, Heinrich Heine Universitat, P O Box 101007, 40001 Dusseldorf, Germany
    J Agric Food Chem 61:2158-64. 2013
    ..of Nrf2, increases ARE-dependent luciferase activity, and enhances the expression of heme oxygenase-1 in Hct116 cells. Additionally, accumulation of the Nrf2 homologue SKN-1 in nuclei of intestinal cells of C...
  100. pmc GATA6 promotes colon cancer cell invasion by regulating urokinase plasminogen activator gene expression
    Narasimhaswamy S Belaguli
    Michael E DeBakey VA Medical Center, Michael E DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA
    Neoplasia 12:856-65. 2010
    ..On the basis of our results, we conclude that GATA6 is an important regulator of uPA gene expression, and the dysregulated expression of GATA6 contributes to colorectal tumorigenesis and tumor invasion...
  101. ncbi Panaxadiol, a purified ginseng component, enhances the anti-cancer effects of 5-fluorouracil in human colorectal cancer cells
    Xiao Li Li
    Department of Anesthesia and Critical Care, Tang Center for Herbal Medicine Research, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA
    Cancer Chemother Pharmacol 64:1097-104. 2009
    ..Panaxadiol (PD) is the purified sapogenin of ginseng saponins, which exhibit anti-tumor activity. In this study, we investigated the possible synergistic anti-cancer effects of PD and 5-FU on a human colorectal cancer cell line, HCT-116...

Research Grants68

  1. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  2. Molecular targeting of PPAR-delta in colon cancer
    Imad Shureiqi; Fiscal Year: 2013
    ..Confirmation of the tested hypothesis could lead to developing PPAR-d-targeted therapy to inhibit tumorigenesis. ..
  3. TAS::75 0849::TAS
    Wenlin Deng; Fiscal Year: 2011
    ..Methods involved are cell culture, in vitro clonogenic assay of tumor cell growth, Xenograft implantation of HCT116 cells and HT29 cells and measuring tumor volume Relevance to public health: The concept proposed in this project, if ..
  4. RECEPTOR-TARGETED COPPER RADIOPHARMACEUTICALS FOR CANCER IMAGING AND THERAPY
    Carolyn J Anderson; Fiscal Year: 2012
    ..Using SSTr2-transfected HCT116 cells, we propose studies to evaluate cell killing with 64Cu-labeled internalizing somatostatin agonists compared to ..
  5. Secretory Patterns of Senescent Tumor Cells
    Igor B Roninson; Fiscal Year: 2011
    ..For this analysis, secreted proteins and paracrine activities will be compared between senescent populations of HCT116 cells and their derivatives where CDKI pathway is inhibited...
  6. Identification of natural products to treat neoplastic disease
    Benjamin Nicholson; Fiscal Year: 2012
    ..Inhibitors of the cancer-related enzyme USP7, a well-described component of this pathway, will be isolated from natural products extracts from the National Cancer Institute for development as novel therapies for neoplastic disease. ..
  7. Characterization of a novel ER membrane protein SPOC
    Ying Huang; Fiscal Year: 2005
    ..Interestingly, constitutive expression of exogenous SPOC in HCT116 cells countered the apoptotic effects of these agents suggesting that SPOC appears to promote cell survival...
  8. QUINONE REDUCTASE REGULATION & QUINONE ANTICANCER DRUGS
    Su Shu Pan; Fiscal Year: 1999
    ....
  9. Development of New GI-specific Histone Deacetylase Inhi*
    Scott Rajski; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  10. MEDIATORS OF INFLAMMATION IN INFLAMMATORY BOWEL DISEASE
    WILLIAM STENSON; Fiscal Year: 2006
    ..Manipulation of the epithelial response to radiation could lead to agents that protect normal epithelial cells during radiation therapy. ..
  11. Novel Proapoptotic BH3-only Proteins in Cancer Apoptosis
    Chien An Hu; Fiscal Year: 2009
    ..By transient expression analysis, we showed that ApoL6, ApoL1 and BOMB promoted cell death in p53-null HCT116 cells. Furthermore, by constructing and characterizing an inducible ApoL6 gene in another p53-null cancer model, DLD-..
  12. Oncogenic Ras, STAT1 and Chemoprevention
    Lidija Klampfer; Fiscal Year: 2009
    ..abstract_text> ..
  13. Cytochrome C Oxidase I:A Biomarker for Colon Cancer Risk
    Claire Payne; Fiscal Year: 2006
    ..Various molecular and cellular techniques will be used in these studies. ..
  14. Preventive of Bile Acid-Induced Colonic Oxidative DNA Damage
    Claire Margaret Payne; Fiscal Year: 2010
    ..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
  15. BIOLOGY OF BAMACAN IN TUMORIGENESIS
    Giancarlo Ghiselli; Fiscal Year: 2006
    ..3) The contribution of bamacan in vivo to colon tumorigenesis by generating transgenic mice over-expressing bamacan in the intestinal epithelium. ..
  16. IGFS & IGF-BINDING PROTEINS IN GROWTH & DIFFERENTIATION
    Pomila Singh; Fiscal Year: 2002
    ....
  17. Tea Polyphenols in Chemoprevention of Prostate Cancer
    SUSANNE MARGARETE HENNING; Fiscal Year: 2011
    ..The results will assist in designing dietary supplements for chemoprevention of early stages of prostate cancer or during watchful waiting. ..
  18. Anticarcinogenic Mechanisms of Tea Constituents
    Zigang Dong; Fiscal Year: 2007
    ....
  19. MODULATION OF CD8 T CELL FUNCTION BY HIV ENVELOPE PROTEI
    Eric Verdin; Fiscal Year: 2003
    ..abstract_text> ..
  20. Anti-cancer effects by the green tea catechins
    SEUNG BAEK; Fiscal Year: 2006
    ..2. Profile changes in gene expression following ECG treatment. 3. Compare the preventative and therapeutic efficacy of individual and combination catechins in animal models of colorectal cancer. ..
  21. Real-time PCR expression profiling of microRNA
    Thomas Schmittgen; Fiscal Year: 2006
    ..The unparallel sensitivity and specificity of real-time PCR as applied to this new and exciting class of regulatory RNAs should propel the field into new directions not only in cancer but in other areas of human health. ..
  22. Screening for Decreased 5-Fluorouracil Catabolism
    Robert B Diasio; Fiscal Year: 2010
    ....
  23. Metabolic Oxidative Stress and TRAIL Cytotoxicity
    Yong Lee; Fiscal Year: 2007
    ..We believe that the successful outcome of this study will support the development and clinical application of TRAIL for the treatment of human cancer. ..
  24. Nanoparticle targeting of cathepsin-L inhibitor and doxorubicin in breast cancer
    Shaker Mousa; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  25. Isopeptide inhibitors for cancer chemotherapy
    Benjamin Nicholson; Fiscal Year: 2008
    ..Compounds that are shown by the assay to inhibit DEN 1 will be evaluated further, and the best of these will be proposed for further development as antitumor agents. [unreadable] [unreadable] [unreadable]..
  26. Bioactive Food Comoponents, Alternative Medicine and Cancer Chemoprevention: Rece
    Shivendra Singh; Fiscal Year: 2007
    ..The theme of our symposium is highly focused and attracts leaders and highly accomplished scientists to discuss promises and challenges in cancer chemoprevention research. [unreadable] [unreadable] [unreadable] [unreadable]..
  27. Nanoscale-Natural Products Chemistry
    TADEUSZ MOLINSKI; Fiscal Year: 2009
    ....
  28. "Toxoplasma gondii: neuro-intestinal interactions"
    Fernando Monroy; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  29. Molecular Approach in Predicting 5-Fluorouracil Efficacy
    Robert Diasio; Fiscal Year: 2005
    ..abstract_text> ..
  30. Enhancing Suicide Gene Therapy Through Mechanism-Based Approaches
    Donna Shewach; Fiscal Year: 2007
    ..The results will aid us in optimizing current gene therapy protocols as well as initiate novel approaches for greater efficacy. ..
  31. Mechanisms of chemopreventive effect of resveratrol
    Zigang Dong; Fiscal Year: 2009
    ..Our long-term goal is to identify the molecular mechanism explaining the anti-tumor effect exhibited by resveratrol. Such knowledge will help in developing better chemopreventive agents with fewer side effects. ..
  32. Study on Ultraviolet-induced Signal Transduction
    Zigang Dong; Fiscal Year: 2010
    ..These preclinical studies are designed to lead to the development of new strategies for the chemoprevention of human skin cancers. ..
  33. INHIBITION OF CARCINOGENESIS BY TEA AND TEA CONSTITUENTS
    Zigang Dong; Fiscal Year: 2010
    ..abstract_text> ..
  34. Growth Factors, Curcumin and Colon Carcinogenesis
    Pomila Singh; Fiscal Year: 2010
    ..The results of these studies are expected to help in developing mechanism-based strategies for preventative/treatment protocols for CRCusing curcumin like agents. ..
  35. PPAR-gamma ligands in colorectal cancer
    Seung Joon Baek; Fiscal Year: 2010
    ..IV. To investigate the in vivo anti-tumorigenic effects of a novel PPARy ligand, MCC-555, and its affects on NAG-1 expression at different stages of colorectal carcinogenesis. ..
  36. ROLE OF CHROMATIN IN HIV TRANSCRIPTIONAL REGULATION
    ERIC M VERDIN; Fiscal Year: 2010
    ..These experiments have the potential of identifying novel factors mediating HIV transcriptional regulation that could be targeted therapeutically in the future. ..
  37. ANTIFUNGALS FROM MARINE INVERTEBRATES--AIDS ADJUVANTS
    Tadeusz F Molinski; Fiscal Year: 2010
    ..Optimized leads identified from those libraries will be advanced to in vitro and in vivo trials. ..
  38. Nutrients and Prostate Cancer Prevention
    Yong Lee; Fiscal Year: 2007
    ..Relevance: We believe that the successful outcome of this study will support the development and clinical application of DATS for the chemoprevention of human prostate cancer. [unreadable] [unreadable] [unreadable]..
  39. GENETIC POLYMORPHISM OF DIHYDROPYRIMIDINE DEHYDROGENASE
    Robert Diasio; Fiscal Year: 2006
    ..g. dihydropyrimidinase. Successful progress on this research project should translate into improved care for patients receiving fluoropyrimidine drugs in the future. ..
  40. Deregulation of Cell Pathways by Ad E1B 55K Oncoproteins
    Daiqing Liao; Fiscal Year: 2005
    ..We will elucidate how E1B perturbs this pathway. These studies will enhance our understanding of cell transformation and cancer. ..
  41. Bioavailability of Tea Polyphenols in Prostate Cancer
    Susanne Henning; Fiscal Year: 2002
    ..ODC enzyme activity will be determined measuring the release of 14C CO2 from 14C ornithine. These studies will provide information important in designing studies to critically test the role of dietary supplementation in prostate cancer. ..
  42. Transcription Regulation and Biological Function of NAG1
    SEUNG BAEK; Fiscal Year: 2005
    ..Cancer and inflammation are certainly environmentally relevant problems and thus this project will provide new clues to aid in the understanding of how these drugs alter tumor development. ..
  43. Micro RNA Expression and Cancer
    Thomas Schmittgen; Fiscal Year: 2005
    ..Assays developed with this research may help to identify the function of a new class of genes in humans. ..
  44. REGULATION OF MACROPHAGE INOS EXPRESSION
    Ming hai Wang; Fiscal Year: 2001
    ..Second it will facilitate understanding of the role of MSP in regulating macrophage activities in inflammation. Further, this work may lead to novel approaches for decreasing tissue destruction in inflammation. ..
  45. "Innate intestinal responses in murine toxoplasmosis"
    Fernando Monroy; Fiscal Year: 2005
    ..abstract_text> ..
  46. Natural Product Antitumor Inducers of Apoptosis
    TADEUSZ MOLINSKI; Fiscal Year: 2004
    ..abstract_text> ..
  47. A New In Vitro Model for HIV Latency
    Eric Verdin; Fiscal Year: 2004
    ..These studies should further establish the validity of our model for the study of HIV latency in patients. ..
  48. b-TrCP E3 Ligase in CLL
    Benjamin Nicholson; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  49. Linking folate deficiency to changes in gene expression
    KAREN KATULA; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  50. UNDERGRADUATE STUDENT TRAINING ACADEMIC RESEARCH
    Fernando Monroy; Fiscal Year: 2006
    ..We strongly feel that a solid science curriculum combined with quality research can reinforce and strengthen the student's determination to pursue graduate training leading to a Ph.D. in a biomedically related science. ..
  51. AUTOCRINE ROLE OF GASTRIN-LIKE PEPTIDES IN COLON CANCER
    Pomila Singh; Fiscal Year: 2002
    ..If we are successful in purifying the novel GP-R (and confirm its role in mediating the mitogenic effects of gastrins) then one can develop systemic strategies to block the receptor mediated effects in future funding periods. ..
  52. MODULATION OF T CELL FUNCTIONS BY HIV1 TAT
    Eric Verdin; Fiscal Year: 2001
    ..These studies will further define the role of Tat as modulator of T cell differentiated functions and establish its role in HIV pathogenesis. ..
  53. STRESS RESPONSES IN MAMMALIAN CELLS
    Yong Lee; Fiscal Year: 2001
    ....
  54. DNA REPAIR GENE POLYMORPHISMS AND BREAST CANCER RISK
    TIMOTHY JORGENSEN; Fiscal Year: 2003
    ..abstract_text> ..
  55. Role of Glutaredoxin in Metabolic Oxidative Stress
    Yong Lee; Fiscal Year: 2007
    ..We believe that investigating the mechanisms of metabolic oxidative stress responses in tumor cells will provide insight into how tumor cells recognize metabolic oxidative stress and initiate signal transduction. ..
  56. Molecular basis of arsenic-induced cell transformation
    Zigang Dong; Fiscal Year: 2007
    ..Such knowledge will facilitate the design of more effective and specific strategies with fewer side effects for chemoprevention of arsenic-induced cancer. ..
  57. Regulation of HIV transcription and replication by SDF-1gamma
    Eric Verdin; Fiscal Year: 2006
    ..We will determine whether a correlations exist between SDF-1gamma expression, HIV replication and the presence of the SDF1-3'A allele. ..
  58. Regulation of NF-kappaB2 by TSA: Role of Acetylation
    Jing Hu; Fiscal Year: 2007
    ..In addition, we will determine whether p52 acetylation affects the binding of transcription factors Sp-1, AP-1, CREB to cyclin D1 promoter using EMSA and chromatin immunoprecipitation (ChIP). ..
  59. Survivin expression and cancer cell drug resistance
    Fengzhi Li; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  60. Substrate specific ERK docking domain inhibitors
    Paul S Shapiro; Fiscal Year: 2010
    ..These compounds will be appropriate for research tools and the development into novel chemotherapeutic agents aimed at treating cancers associated with elevated ERK pathway activity. ..
  61. NOVEL PROBES FOR IMMUNOPHILIN MEDIATED CELL CONTROL
    TADEUSZ MOLINSKI; Fiscal Year: 2001
    ..5. To understand how modulation of the FKBP12/ryanodine receptor complex by bastadins influences cellular growth and differentiation using the BC3H1 cell line as model. ..
  62. CONJUGATED LINOLEIC AND OXIDATIVE LIPID METABOLISM
    ARTHUR BULL; Fiscal Year: 2001
    ..Metabolic pathways and biological activity will have been evaluated. The results may provide insight into approaches for the reduction of mammary cancer in humans. ..
  63. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  64. Research Network for Drug-Induced Liver Disease
    Petr Protiva; Fiscal Year: 2007
    ..The clinico-pathological database and the tissue and DNA repositories will permit us to create and test numerous hypotheses relating to the pathogenesis and pharmacogenomics of DILD. ..
  65. SURGICAL STUDIES OF ONTOGENY, AGING AND THE GUT
    BERNARD EVERS; Fiscal Year: 2001
    ..In addition, analysis of these cellular processes will provide a better understanding of not only normal gut development and function but may also provide a model to better elucidate the cellular events leading to gut neoplasia. ..
  66. Surgical Studies of Aging and the Gut
    BERNARD EVERS; Fiscal Year: 2009
    ..abstract_text> ..