tumor cell line

Summary

Summary: A cell line derived from cultured tumor cells.

Top Publications

  1. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
  2. ncbi EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy
    J Guillermo Paez
    Departments of Medical Oncology and Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 304:1497-500. 2004
  3. pmc Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles
    Aravind Subramanian
    Broad Institute of Massachusetts Institute of Technology and Harvard, 320 Charles Street, Cambridge, MA 02141, USA
    Proc Natl Acad Sci U S A 102:15545-50. 2005
  4. pmc A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes
    Richard M Neve
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94270, USA
    Cancer Cell 10:515-27. 2006
  5. ncbi A human colon cancer cell capable of initiating tumour growth in immunodeficient mice
    Catherine A O'Brien
    Division of Cell and Molecular Biology, University Health Network, Toronto, Ontario, M5G 1L7, Canada
    Nature 445:106-10. 2007
  6. ncbi Identification and expansion of human colon-cancer-initiating cells
    Lucia Ricci-Vitiani
    Department of Hematology and Oncology, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    Nature 445:111-5. 2007
  7. ncbi The consensus coding sequences of human breast and colorectal cancers
    Tobias Sjoblom
    Ludwig Center and Howard Hughes Medical Institute, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Science 314:268-74. 2006
  8. ncbi The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease
    Justin Lamb
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    Science 313:1929-35. 2006
  9. ncbi Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer
    Scott A Tomlins
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, Ann Arbor, MI 48109 0602, USA
    Science 310:644-8. 2005
  10. ncbi Tumour invasion and metastasis initiated by microRNA-10b in breast cancer
    Li Ma
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 449:682-8. 2007

Detail Information

Publications263 found, 100 shown here

  1. ncbi Identification of human brain tumour initiating cells
    Sheila K Singh
    The Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, M5G 1X8, Canada
    Nature 432:396-401. 2004
    ....
  2. ncbi EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy
    J Guillermo Paez
    Departments of Medical Oncology and Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 304:1497-500. 2004
    ..These results suggest that EGFR mutations may predict sensitivity to gefitinib...
  3. pmc Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles
    Aravind Subramanian
    Broad Institute of Massachusetts Institute of Technology and Harvard, 320 Charles Street, Cambridge, MA 02141, USA
    Proc Natl Acad Sci U S A 102:15545-50. 2005
    ..The GSEA method is embodied in a freely available software package, together with an initial database of 1,325 biologically defined gene sets...
  4. pmc A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes
    Richard M Neve
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94270, USA
    Cancer Cell 10:515-27. 2006
    ..We show, using Trastuzumab (Herceptin) monotherapy as an example, that the system can be used to identify molecular features that predict or indicate response to targeted therapies or other physiological perturbations...
  5. ncbi A human colon cancer cell capable of initiating tumour growth in immunodeficient mice
    Catherine A O'Brien
    Division of Cell and Molecular Biology, University Health Network, Toronto, Ontario, M5G 1L7, Canada
    Nature 445:106-10. 2007
    ....
  6. ncbi Identification and expansion of human colon-cancer-initiating cells
    Lucia Ricci-Vitiani
    Department of Hematology and Oncology, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy
    Nature 445:111-5. 2007
    ..We conclude that colorectal cancer is created and propagated by a small number of undifferentiated tumorigenic CD133+ cells, which should therefore be the target of future therapies...
  7. ncbi The consensus coding sequences of human breast and colorectal cancers
    Tobias Sjoblom
    Ludwig Center and Howard Hughes Medical Institute, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USA
    Science 314:268-74. 2006
    ..These data define the genetic landscape of two human cancer types, provide new targets for diagnostic and therapeutic intervention, and open fertile avenues for basic research in tumor biology...
  8. ncbi The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease
    Justin Lamb
    Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02142, USA
    Science 313:1929-35. 2006
    ..These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project...
  9. ncbi Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer
    Scott A Tomlins
    Department of Pathology, University of Michigan Medical School, 1301 Catherine Street, Ann Arbor, MI 48109 0602, USA
    Science 310:644-8. 2005
    ..These results have implications in the development of carcinomas and the molecular diagnosis and treatment of prostate cancer...
  10. ncbi Tumour invasion and metastasis initiated by microRNA-10b in breast cancer
    Li Ma
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 449:682-8. 2007
    ....
  11. ncbi MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling
    Jeffrey A Engelman
    Massachusetts General Hospital Cancer Center, Boston, MA 02114, USA
    Science 316:1039-43. 2007
    ..Thus, we propose that MET amplification may promote drug resistance in other ERBB-driven cancers as well...
  12. ncbi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex
    D D Sarbassov
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Science 307:1098-101. 2005
    ..Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation...
  13. ncbi Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis
    Jing Yang
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Cell 117:927-39. 2004
    ..These results establish a mechanistic link between Twist, EMT, and tumor metastasis...
  14. ncbi In vivo activation of the p53 pathway by small-molecule antagonists of MDM2
    Lyubomir T Vassilev
    Department of Discovery Oncology, Roche Research Center, Hoffmann La Roche, Inc, Nutley, NJ 07110, USA
    Science 303:844-8. 2004
    ..These compounds bind MDM2 in the p53-binding pocket and activate the p53 pathway in cancer cells, leading to cell cycle arrest, apoptosis, and growth inhibition of human tumor xenografts in nude mice...
  15. ncbi Mesenchymal stem cells within tumour stroma promote breast cancer metastasis
    Antoine E Karnoub
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 449:557-63. 2007
    ..Collectively, these data demonstrate that the tumour microenvironment facilitates metastatic spread by eliciting reversible changes in the phenotype of cancer cells...
  16. ncbi Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase
    Helen E Bryant
    The Institute for Cancer Studies, University of Sheffield, Medical School, Beech Hill Road, Sheffield S10 2RX, UK
    Nature 434:913-7. 2005
    ..The use of an inhibitor of a DNA repair enzyme alone to selectively kill a tumour, in the absence of an exogenous DNA-damaging agent, represents a new concept in cancer treatment...
  17. ncbi An inhibitor of Bcl-2 family proteins induces regression of solid tumours
    Tilman Oltersdorf
    Idun Pharmaceuticals, 9380 Judicial Drive, San Diego, California 92121, USA
    Nature 435:677-81. 2005
    ....
  18. ncbi A microRNA polycistron as a potential human oncogene
    Lin He
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 435:828-33. 2005
    ..Together, these studies indicate that non-coding RNAs, specifically microRNAs, can modulate tumour formation, and implicate the mir-17-92 cluster as a potential human oncogene...
  19. pmc Production of infectious hepatitis C virus in tissue culture from a cloned viral genome
    Takaji Wakita
    Department of Microbiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183 8526, Japan
    Nat Med 11:791-6. 2005
    ..The cell culture-generated HCV is infectious for chimpanzee. This system provides a powerful tool for studying the viral life cycle and developing antiviral strategies...
  20. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
    ..Thus, ERK and Rho constitute part of an integrated mechanoregulatory circuit linking matrix stiffness to cytoskeletal tension through integrins to regulate tissue phenotype...
  21. pmc Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis
    Tsung Cheng Chang
    The McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Cell 26:745-52. 2007
    ..Therefore, it is likely that an important function of miR-34a is the modulation and fine-tuning of the gene expression program initiated by p53...
  22. ncbi Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures
    Jayanta Debnath
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Methods 30:256-68. 2003
    ....
  23. ncbi Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer
    Klarisa Rikova
    Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA
    Cell 131:1190-203. 2007
    ..By focusing on activated cell circuitry, the approach outlined here provides insight into cancer biology not available at the chromosomal and transcriptional levels and can be applied broadly across all human cancers...
  24. ncbi Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion
    Akira Orimo
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 121:335-48. 2005
    ..Our findings indicate that fibroblasts within invasive breast carcinomas contribute to tumor promotion in large part through the secretion of SDF-1...
  25. ncbi let-7 regulates self renewal and tumorigenicity of breast cancer cells
    Fengyan Yu
    Department of Breast Surgery, No 2 Affiliated Hospital, Sun Yat Sen University, Guangzhou 510120, People s Republic of China
    Cell 131:1109-23. 2007
    ..Therefore let-7 regulates multiple BT-IC stem cell-like properties by silencing more than one target...
  26. ncbi MicroRNA signatures in human ovarian cancer
    Marilena V Iorio
    Molecular Biology Unit, Department of Experimental Oncology, Fondazione Istituti di Ricovero e Cura a Carattere Scientifico, Istituto Nazionale Tumori, Milano, Italy
    Cancer Res 67:8699-707. 2007
    ..Our results indicate that miRNAs might play a role in the pathogenesis of human EOC and identify altered miRNA gene methylation as a possible epigenetic mechanism involved in their aberrant expression...
  27. ncbi Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions
    Vassilis G Gorgoulis
    Department of Histology and Embryology, School of Medicine, University of Athens, Athens GR 11527, Greece
    Nature 434:907-13. 2005
    ..We propose that, from its earliest stages, cancer development is associated with DNA replication stress, which leads to DNA double-strand breaks, genomic instability and selective pressure for p53 mutations...
  28. pmc Widespread microRNA repression by Myc contributes to tumorigenesis
    Tsung Cheng Chang
    McKusick Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Nat Genet 40:43-50. 2008
    ..We further show that enforced expression of repressed miRNAs diminishes the tumorigenic potential of lymphoma cells. These results demonstrate that extensive reprogramming of the miRNA transcriptome by Myc contributes to tumorigenesis...
  29. ncbi DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis
    Jirina Bartkova
    Institute of Cancer Biology and Centre for Genotoxic Stress Research, Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Nature 434:864-70. 2005
    ..Mutations compromising this checkpoint, including defects in the ATM-Chk2-p53 pathway, might allow cell proliferation, survival, increased genomic instability and tumour progression...
  30. ncbi MicroRNA expression profiling in prostate cancer
    Kati P Porkka
    Laboratory of Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland
    Cancer Res 67:6130-5. 2007
    ....
  31. ncbi Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy
    Lionel Apetoh
    Institut Gustave Roussy IGR, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Nat Med 13:1050-9. 2007
    ..These results delineate a clinically relevant immunoadjuvant pathway triggered by tumor cell death...
  32. pmc The colorectal microRNAome
    Jordan M Cummins
    The Sidney Kimmel Comprehensive Cancer Center and Howard Hughes Medical Institute, Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA
    Proc Natl Acad Sci U S A 103:3687-92. 2006
    ..These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues...
  33. ncbi Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties
    Dario Ponti
    Department of Experimental Oncology and Laboratories, Istituto Nazionale Tumori, Milan, Italy
    Cancer Res 65:5506-11. 2005
    ....
  34. pmc Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions
    Adrian P Bracken
    Biotech Research and Innovation Centre BRIC, 2100 Copenhagen Ø, Denmark
    Genes Dev 20:1123-36. 2006
    ....
  35. pmc Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis
    Angie M Cheng
    Ambion, Inc 2130 Woodward Street, Austin, TX 78744 1832, USA
    Nucleic Acids Res 33:1290-7. 2005
    ..From these screens, we conclude that miRNA-mediated regulation has a complexity of cellular outcomes and that miRNAs can be mediators of regulation of cell growth and apoptosis pathways...
  36. ncbi The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses
    Mitsutoshi Yoneyama
    Department of Tumor Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, 3 18 22 Honkomagome, Bunkyo ku, Tokyo 113 8613, Japan
    Nat Immunol 5:730-7. 2004
    ..Subsequent gene activation by these factors induced antiviral functions, including type I interferon production. Thus, RIG-I is key in the detection and subsequent eradication of the replicating viral genomes...
  37. pmc Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma
    Gentao Liu
    Maxine Dunitz Neurosurgical Institute, Cedars Sinai Medical Center, Los Angeles, CA, 90048, USA
    Mol Cancer 5:67. 2006
    ..The gene expression and the drug resistance property of CD133 positive cancer stem cells, however, are still unknown...
  38. ncbi MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells
    Jennifer A Chan
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 65:6029-33. 2005
    ..Our data suggest that aberrantly expressed miR-21 may contribute to the malignant phenotype by blocking expression of critical apoptosis-related genes...
  39. ncbi Transcriptional activation of miR-34a contributes to p53-mediated apoptosis
    Nina Raver-Shapira
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell 26:731-43. 2007
    ..Perturbation of miR-34a expression, as occurs in some human cancers, may thus contribute to tumorigenesis by attenuating p53-dependent apoptosis...
  40. pmc Autophagy inhibition enhances therapy-induced apoptosis in a Myc-induced model of lymphoma
    Ravi K Amaravadi
    Division of Hematology Oncology, Department of Medicine, Abramson Family Cancer Research Institute, Philadelphia, Pennsylvania 19104, USA
    J Clin Invest 117:326-36. 2007
    ....
  41. ncbi Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter
    Raphaël Métivier
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Cell 115:751-63. 2003
    ....
  42. ncbi Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer
    Patrick C Hermann
    Department of Surgery, Ludwig Maximilians University, 81377 Munich, Germany
    Cell Stem Cell 1:313-23. 2007
    ..Strategies aimed at modulating the SDF-1/CXCR4 axis may have important clinical applications to inhibit metastasis of cancer stem cells...
  43. ncbi MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer
    Fanyin Meng
    Department of Internal Medicine, Scott and White Clinic, Texas A and M University System Health Science Center College of Medicine, Temple, Texas, USA
    Gastroenterology 133:647-58. 2007
    ..Although a role for aberrant miRNA expression in cancer has been postulated, the pathophysiologic role and relevance of aberrantly expressed miRNA to tumor biology has not been established...
  44. ncbi Colon cancer stem cells dictate tumor growth and resist cell death by production of interleukin-4
    Matilde Todaro
    Department of Surgical and Oncological Sciences, Cellular and Molecular Pathophysiology Laboratory, University of Palermo, 90127 Palermo, Italy
    Cell Stem Cell 1:389-402. 2007
    ..Our data suggest that colon tumor growth is dictated by stem-like cells that are treatment resistant due to the autocrine production of IL-4...
  45. ncbi A tumorigenic subpopulation with stem cell properties in melanomas
    Dong Fang
    Program of Molecular and Cellular Oncogenesis, The Wistar Institute and Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, PA 19104, USA
    Cancer Res 65:9328-37. 2005
    ..Based on these findings, we propose that melanomas can contain a subpopulation of stem cells that contribute to heterogeneity and tumorigenesis. Targeting this population may lead to effective treatments for melanomas...
  46. pmc Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells
    Jing Tan
    Molecular Pharmacology, Genome Institute of Singapore, 138672, Singapore
    Genes Dev 21:1050-63. 2007
    ..Our results demonstrate the unique feature of DZNep as a novel chromatin remodeling compound and suggest that pharmacologic reversal of PRC2-mediated gene repression by DZNep may constitute a novel approach for cancer therapy...
  47. ncbi Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members
    Michael Certo
    Department of Medical Oncology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Cancer Cell 9:351-65. 2006
    ..Our data allow us to distinguish a cellular state we call "primed for death," which can be determined by BH3 profiling and which correlates with dependence on antiapoptotic family members for survival...
  48. ncbi Akt stimulates aerobic glycolysis in cancer cells
    Rebecca L Elstrom
    Department of Cancer Biology, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Cancer Res 64:3892-9. 2004
    ....
  49. ncbi miR-221 and miR-222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1
    Silvia Galardi
    Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Via Montpellier, 1 00133 Rome, Italy
    J Biol Chem 282:23716-24. 2007
    ....
  50. pmc Accumulation of miR-155 and BIC RNA in human B cell lymphomas
    Peggy S Eis
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:3627-32. 2005
    ..Because patients with activated B cell-type DLBCL have a poorer clinical prognosis, quantification of this microRNA may be diagnostically useful...
  51. pmc The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression
    Paraic A Kenny
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    Mol Oncol 1:84-96. 2007
    ..We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments...
  52. ncbi Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB
    Dos D Sarbassov
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Mol Cell 22:159-68. 2006
    ..Our work describes an unforeseen mechanism of action for rapamycin that suggests it can be used to inhibit Akt/PKB in certain cell types...
  53. ncbi p53 regulates mitochondrial respiration
    Satoaki Matoba
    Cardiology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Science 312:1650-3. 2006
    ..That SCO2 couples p53 to mitochondrial respiration provides a possible explanation for the Warburg effect and offers new clues as to how p53 might affect aging and metabolism...
  54. ncbi Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer
    Hiromu Suzuki
    Division of Tumor Biology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, 1650 Orleans Street, Baltimore, Maryland 21231, USA
    Nat Genet 36:417-22. 2004
    ....
  55. pmc Genes that mediate breast cancer metastasis to lung
    Andy J Minn
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 436:518-24. 2005
    ..Others contribute to aggressive growth selectively in the lung. Many encode extracellular proteins and are of previously unknown relevance to cancer metastasis...
  56. ncbi MicroRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1)
    Shuomin Zhu
    Department of Medical Microbiology, Immunology, and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794, USA
    J Biol Chem 282:14328-36. 2007
    ..Thus, down-regulation of TPM1 by mir-21 may explain, at least in part, why suppression of mir-21 can inhibit tumor growth, further supporting the notion that mir-21 functions as an oncogene...
  57. ncbi Epithelial--mesenchymal and mesenchymal--epithelial transitions in carcinoma progression
    Honor Hugo
    Embryology Laboratory, Murdoch Children s Research Institute, The Royal Children s Hospital, Victoria, Australia
    J Cell Physiol 213:374-83. 2007
    ..Finally, perspectives from a clinical and translational viewpoint are discussed...
  58. ncbi MicroRNA-34b and MicroRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth
    David C Corney
    Department of Biomedical Sciences and Microarray Core Facility, Cornell University, Ithaca, New York, USA
    Cancer Res 67:8433-8. 2007
    ..Taken together, these results show the existence of a novel mechanism by which p53 suppresses such critical components of neoplastic growth as cell proliferation and adhesion-independent colony formation...
  59. pmc A distinct "side population" of cells with high drug efflux capacity in human tumor cells
    C Hirschmann-Jax
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children s Hospital, and DeBakey Department of Surgery, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 101:14228-33. 2004
    ....
  60. ncbi Identification and characterization of tumorigenic liver cancer stem/progenitor cells
    Stephanie Ma
    Department of Pathology, The University of Hong Kong, Pokfulam, Hong Kong, China
    Gastroenterology 132:2542-56. 2007
    ..The aim of this study is to identify, isolate, and characterize the CSC population that drives and maintains hepatocellular carcinoma (HCC) growth and metastasis...
  61. ncbi Side population is enriched in tumorigenic, stem-like cancer cells, whereas ABCG2+ and ABCG2- cancer cells are similarly tumorigenic
    Lubna Patrawala
    Department of Carcinogenesis, Science Park Research Division, The University of Texas M D Anderson Cancer Center, Smithville, Texas 78957, USA
    Cancer Res 65:6207-19. 2005
    ....
  62. ncbi Attenuation of LDH-A expression uncovers a link between glycolysis, mitochondrial physiology, and tumor maintenance
    Valeria R Fantin
    Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA
    Cancer Cell 9:425-34. 2006
    ..These results demonstrate that LDH-A plays a key role in tumor maintenance...
  63. ncbi Epithelial-mesenchymal transition in breast cancer relates to the basal-like phenotype
    David Sarrio
    Breast and Gynecological Cancer Group, Molecular Pathology Programme, Centro Nacional de Investigaciones Oncologicas, Madrid, Spain
    Cancer Res 68:989-97. 2008
    ....
  64. ncbi Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia
    Andrew P Weng
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Science 306:269-71. 2004
    ..These findings greatly expand the role of activated NOTCH1 in the molecular pathogenesis of human T-ALL and provide a strong rationale for targeted therapies that interfere with NOTCH signaling...
  65. pmc Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation
    Christine Mayr
    Howard Hughes Medical Institute and Department of Biology, Massachusetts Institute of Technology, and Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 315:1576-9. 2007
    ..Thus, losing miRNA-directed repression of an oncogene provides a mechanism for tumorigenesis, and disrupting a single miRNA-target interaction can produce an observable phenotype in mammalian cells...
  66. ncbi A functional genetic approach identifies the PI3K pathway as a major determinant of trastuzumab resistance in breast cancer
    Katrien Berns
    Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cancer Cell 12:395-402. 2007
    ..Thus, assessment of PI3K pathway activation may provide a biomarker to identify patients unlikely to respond to trastuzumab-based therapy...
  67. ncbi Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma
    Rossella Galli
    Stem Cell Research Institute and Laboratory of Molecular Diagnostics, H S Raffaele, Milan, Italy
    Cancer Res 64:7011-21. 2004
    ..Thus, cells possessing all of the characteristics expected from tumor neural stem cells seem to be involved in the growth and recurrence of adult human glioblastomas multiforme...
  68. ncbi Diagnostic and prognostic microRNAs in stage II colon cancer
    Troels Schepeler
    Department of Clinical Biochemistry, Molecular Diagnostic Laboratory, Aarhus University Hospital, Aarhus N, Denmark
    Cancer Res 68:6416-24. 2008
    ....
  69. pmc Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex
    Donna D Zhang
    Department of Biochemistry, University of Missouri Columbia, M121 Medical Sciences Building, Columbia, MO 65212, USA
    Mol Cell Biol 24:10941-53. 2004
    ....
  70. ncbi Complete replication of hepatitis C virus in cell culture
    Brett D Lindenbach
    Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 309:623-6. 2005
    ..HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals...
  71. pmc Three-dimensional culture models of normal and malignant breast epithelial cells
    Genee Y Lee
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Nat Methods 4:359-65. 2007
    ..Alternatively, the 3D 'on-top' assay, in which cells are cultured on top of a thin lrECM gel overlaid with a dilute solution of lrECM, may be used as described in Box 2 (Fig. 1 and Fig. 2)...
  72. pmc Identification of cells initiating human melanomas
    Tobias Schatton
    Transplantation Research Center, Children s Hospital Boston and Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 451:345-9. 2008
    ..Identification of tumour-initiating cells with enhanced abundance in more advanced disease but susceptibility to specific targeting through a defining chemoresistance determinant has important implications for cancer therapy...
  73. ncbi Distinct classes of chromosomal rearrangements create oncogenic ETS gene fusions in prostate cancer
    Scott A Tomlins
    Michigan Center for Translational Pathology, Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nature 448:595-9. 2007
    ..Furthermore, the identification of androgen-repressed and insensitive 5' fusion partners may have implications for the anti-androgen treatment of advanced prostate cancer...
  74. pmc The tumor suppressor microRNA let-7 represses the HMGA2 oncogene
    Yong Sun Lee
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, USA
    Genes Dev 21:1025-30. 2007
    ....
  75. ncbi DRAM, a p53-induced modulator of autophagy, is critical for apoptosis
    Diane Crighton
    Tumour Cell Death Laboratory, Beatson Institute for Cancer Research, Cancer Research UK Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK
    Cell 126:121-34. 2006
    ..Collectively therefore, these studies not only report a stress-induced regulator of autophagy but also highlight the relationship of DRAM and autophagy to p53 function and damage-induced programmed cell death...
  76. ncbi A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth
    Sebastien Bonnet
    Pulmonary Hypertension Program and Vascular Biology Group, Department of Physiology, University of Alberta, Edmonton, AB T6G 2B7, Canada
    Cancer Cell 11:37-51. 2007
    ..Molecular inhibition of PDK2 by siRNA mimics DCA. The mitochondria-NFAT-Kv axis and PDK are important therapeutic targets in cancer; the orally available DCA is a promising selective anticancer agent...
  77. ncbi Oncogenic pathway signatures in human cancers as a guide to targeted therapies
    Andrea H Bild
    Institute for Genome Sciences and Policy, Duke University, Durham, North Carolina 27708, USA
    Nature 439:353-7. 2006
    ..Linking pathway deregulation with sensitivity to therapeutics that target components of the pathway provides an opportunity to make use of these oncogenic pathway signatures to guide the use of targeted therapeutics...
  78. ncbi Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells
    Li Bing Song
    State Key Laboratory of Oncology in Southern China, Sun Yat sen University Cancer Center, Guangzhou, China
    Cancer Res 66:6225-32. 2006
    ....
  79. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
    ..These findings provide a direct mechanistic link between extra centrosomes and CIN, two common characteristics of solid tumours. We propose that this mechanism may be a common underlying cause of CIN in human cancer...
  80. pmc Chromatin structure analyses identify miRNA promoters
    Fatih Ozsolak
    Department of Dermatology and Cutaneous Biology Research Center, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Genes Dev 22:3172-83. 2008
    ..The high-throughput identification of miRNA promoter and enhancer regulatory elements sheds light on evolution of miRNA transcription and permits rapid identification of transcriptional networks of miRNAs...
  81. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
    ..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
  82. pmc Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis
    Sarah P Thayer
    Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 425:851-6. 2003
    ..These data suggest that this pathway may have an early and critical role in the genesis of this cancer, and that maintenance of hedgehog signalling is important for aberrant proliferation and tumorigenesis...
  83. pmc BRAF mutation predicts sensitivity to MEK inhibition
    David B Solit
    Department of Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA
    Nature 439:358-62. 2006
    ..These data suggest an exquisite dependency on MEK activity in BRAF mutant tumours, and offer a rational therapeutic strategy for this genetically defined tumour subtype...
  84. ncbi Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells
    L Patrawala
    Department of Carcinogenesis, The University of Texas MD Anderson Cancer Center, Science Park Research Division, Smithville, 78957, USA
    Oncogene 25:1696-708. 2006
    ..Altogether, our results suggest that the CD44(+) PCa cell population is enriched in tumorigenic and metastatic progenitor cells...
  85. pmc Endogenous human microRNAs that suppress breast cancer metastasis
    Sohail F Tavazoie
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 451:147-52. 2008
    ..miR-335 and miR-126 are thus identified as metastasis suppressor microRNAs in human breast cancer...
  86. pmc Epigenetic silencing of tumour suppressor gene p15 by its antisense RNA
    Wenqiang Yu
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland 21205, USA
    Nature 451:202-6. 2008
    ..Thus, natural antisense RNA may be a trigger for heterochromatin formation and DNA methylation in TSG silencing in tumorigenesis...
  87. ncbi PTEN activation contributes to tumor inhibition by trastuzumab, and loss of PTEN predicts trastuzumab resistance in patients
    Yoichi Nagata
    Department of Surgical Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Cancer Cell 6:117-27. 2004
    ..Thus, PTEN deficiency is a powerful predictor for trastuzumab resistance. Additionally, PI3K inhibitors rescued PTEN loss-induced trastuzumab resistance, suggesting that PI3K-targeting therapies could overcome this resistance...
  88. ncbi Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells
    Lisa B Frankel
    Biotech Research and Innovation Centre, Bioinformatics Centre, University of Copenhagen, DK 2200 N Copenhagen, Denmark
    J Biol Chem 283:1026-33. 2008
    ..We furthermore found that the tumor suppressor protein Programmed Cell Death 4 (PDCD4) is regulated by miR-21 and demonstrated that PDCD4 is a functionally important target for miR-21 in breast cancer cells...
  89. pmc Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase
    Fan Yeung
    Department of Biochemistry and Molecular Genetics, The University of Virginia, Charlottesville, VA 22908, USA
    EMBO J 23:2369-80. 2004
    ....
  90. pmc Cancer-associated stromal fibroblasts promote pancreatic tumor progression
    Rosa F Hwang
    Department of Surgical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77230 1402, USA
    Cancer Res 68:918-26. 2008
    ..These data indicate that stellate cells have an important role in supporting and promoting pancreatic cancer. Identification of HPSC-derived factors may lead to novel stroma-targeted therapies for pancreatic cancer...
  91. ncbi Dual regulation of Snail by GSK-3beta-mediated phosphorylation in control of epithelial-mesenchymal transition
    Binhua P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 6:931-40. 2004
    ..Furthermore, inhibition of GSK-3beta results in the upregulation of Snail and downregulation of E-cadherin in vivo. Thus, Snail and GSK-3beta together function as a molecular switch for many signalling pathways that lead to EMT...
  92. pmc MicroRNA 21 promotes glioma invasion by targeting matrix metalloproteinase regulators
    Galina Gabriely
    Center of Neurologic Diseases, Brigham and Women s Hospital, 4 Blackfan Circle, HIM 758, Boston, MA 02115, USA
    Mol Cell Biol 28:5369-80. 2008
    ....
  93. ncbi MicroRNA-34a functions as a potential tumor suppressor by inducing apoptosis in neuroblastoma cells
    C Welch
    Children s Cancer Research Institute and Department of Pediatrics, The University of Texas Health Science Center at San Antonio, MC 7784, TX, USA
    Oncogene 26:5017-22. 2007
    ..Thus, adding to the increasing role of miRNAs in cancer, miR-34a may act as a suppressor of NB tumorgenesis...
  94. pmc Regulation of the p27(Kip1) tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation
    Carlos le Sage
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO J 26:3699-708. 2007
    ..Thus, deregulated expression of miR-221&222 promotes cancerous growth by inhibiting the expression of p27(Kip1)...
  95. pmc Discovery of a selective inhibitor of oncogenic B-Raf kinase with potent antimelanoma activity
    James Tsai
    Plexxikon, Inc, 91 Bolivar Drive, Berkeley, CA 94710, USA
    Proc Natl Acad Sci U S A 105:3041-6. 2008
    ....
  96. ncbi Vascular normalization by vascular endothelial growth factor receptor 2 blockade induces a pressure gradient across the vasculature and improves drug penetration in tumors
    Ricky T Tong
    Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Cancer Res 64:3731-6. 2004
    ..Thus, vascular normalization may contribute to the improved survival rates in tumor-bearing animals and in colorectal carcinoma patients treated with an anti-VEGF antibody in combination with cytotoxic therapies...
  97. pmc Braf(V600E) cooperates with Pten loss to induce metastatic melanoma
    David Dankort
    Cancer Research Institute and Department of Cell and Molecular Pharmacology, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, USA
    Nat Genet 41:544-52. 2009
    ..These mice, engineered with a common genetic profile to human melanoma, provide a system to study melanoma's cardinal feature of metastasis and for preclinical evaluation of agents designed to prevent or treat metastatic disease...
  98. pmc Regulation of autophagy by cytoplasmic p53
    Ezgi Tasdemir
    INSERM, U848, France
    Nat Cell Biol 10:676-87. 2008
    ..These results provide evidence of a key signalling pathway that links autophagy to the cancer-associated dysregulation of p53...
  99. ncbi PIK3CA mutations correlate with hormone receptors, node metastasis, and ERBB2, and are mutually exclusive with PTEN loss in human breast carcinoma
    Lao H Saal
    Integrated Program in Cellular, Molecular, and Biophysical Studies, Institute for Cancer Genetics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Cancer Res 65:2554-9. 2005
    ..Thus, mutation of PIK3CA is frequent, occurs early in carcinoma development, and has prognostic and therapeutic implications...
  100. ncbi Side population purified from hepatocellular carcinoma cells harbors cancer stem cell-like properties
    Tetsuhiro Chiba
    Department of Regenerative Medicine, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan
    Hepatology 44:240-51. 2006
    ..In conclusion, we propose that a minority population, detected as SP cells in HCC cells, possess extreme tumorigenic potential and provide heterogeneity to the cancer stem cell system characterized by distinct hierarchy...
  101. ncbi Lysyl oxidase is essential for hypoxia-induced metastasis
    Janine T Erler
    Department of Radiation Oncology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 440:1222-6. 2006
    ..Furthermore, LOX may be required to create a niche permissive for metastatic growth. Our findings indicate that LOX is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases...

Research Grants62

  1. PTEN TUMOR SUPPRESSOR AND SIGNAL TRANSDUCTION
    RAMON E PARSONS; Fiscal Year: 2012
    ..We will measure the frequency of over expression in tumors and measure the effect of RNAi on tumor cell line growth and signaling...
  2. Identification of LSD1 inhibitors targeting epigenetic regulation in tumor cells
    Patrick M Woster; Fiscal Year: 2013
    ..The cellular effects of all analogues will be monitored in the HCT116 tumor cell line. Each compound will be evaluated alone, and in combination with a DNA methyltransferase inhibitor and/or a ..
  3. Aging Features in Mice with Conditional Expression of p16Ink4a
    Gregory H Enders; Fiscal Year: 2013
    ..in tissue culture, and we have shown that induction of p16 is sufficient to impose senescence in a cultured tumor cell line. Genetic analyses using p16-null mice revealed that p16 limits proliferation of progenitor cells in several ..
  4. HDAC6-Selective Inhibitors for Pancreatic Cancer Treatment
    Werner Tueckmantel; Fiscal Year: 2010
    ..survival in vitro;and (3) to determine the in vivo efficacy of the 5 best HDACIs on established pancreatic tumor cell line xenografts with co-administration of gemcitabine...
  5. Impact of proximal promoter polymorphisms on AHR gene expression in human lung
    David C Spink; Fiscal Year: 2013
    ..We will perform in vitro assays of transcription factor binding and AHR promoter activities in a lung tumor cell line and determine allelic expression of AhR mRNA in normal lung tissue from individuals heterozygous for these ..
  6. DNA vaccines for metastatic breast cancer at old age
    Claudia Gravekamp; Fiscal Year: 2010
    ..The model is based on the injection of a syngeneic mouse mammary tumor cell line into immune competent mice, which then give rise to metastatic breast cancer without immunological rejection...
  7. Discovery of novel PCFT-targeted agents
    Larry H Matherly; Fiscal Year: 2013
    ..In aim 2, we test compounds from aim 1 for cytotoxicity in isogenic hamster and human tumor cell line models with established patterns of RFC and hPCFT expression, identify molecular targets by nucleoside ..
  8. TRANSPLANT AND TUMOR REJECTION PROCESSES WITHIN THE EYE
    Jerry Niederkorn; Fiscal Year: 2013
    ..The proposed studies will use two clones derived from a parental tumor cell line that circumvents immune privilege in the mouse eye...
  9. Effects of Continuous versus Cyclic Oral Contraceptives on Mammary Tumor Growth
    PATRICIA ANN MASSO-WELCH; Fiscal Year: 2013
    ..versus continuous dosing +/- OC to alter the mammary tumor growth using the TM2H transplantable mouse mammary tumor cell line. Mammary tumors from different treatment groups will be compared for alterations in expression of ER, PR, and ..
  10. Purine Synthesis Inhibitors with Selective Folate Receptor Tumor Transport
    Larry H Matherly; Fiscal Year: 2013
    ..In Aim 2, we will evaluate compounds from Aim 1 for cytotoxicity in isogenic hamster and human tumor cell line models with established RFC and FR expression, and will identify molecular mechanisms including cellular ..
  11. LEF/TCF Function in Adult Stem Cell Fate and Tumorigenesis
    Stephen R Lyle; Fiscal Year: 2010
    ..2) Determine the cellular and molecular properties of a new sebaceous tumor cell line showing deficient LEF-1 signaling...
  12. Novel device to study tumor metastasis in vitro
    Sophia K Khaldoyanidi; Fiscal Year: 2010
    ..We propose to use human MDA-MB- 435 tumor cell line as the model since these cells demonstrated high ability to adhere to endothelial cells in vivo and in vitro...
  13. Paxillin as a Liaison between Extranuclear and Intranuclear Steroid Signaling
    Stephen R Hammes; Fiscal Year: 2013
    ..Furthermore, since paxillin actually regulates Erk activation and downstream effects in every tumor cell line tested, regardless of origin, we postulate that paxillin might be a general regulator of Erk action well ..
  14. Exploiting novel uses of gene-modified drug-resistant hematopoietic cells
    H Spencer; Fiscal Year: 2009
    ..and in vivo expansion of HSC-derived immunocompetent cells, where 100% of mice inoculated with a sarcoma tumor cell line achieve long-term cure...
  15. Emerging Technologies Applied to the Discovery of Human Tumor Viruses
    Patrick Moore; Fiscal Year: 2009
    ..To demonstrate the feasibility of this method, we performed pilot studies of DTS on a tumor cell line infected with latent KSHV virus. DTS rapidly and uniquely identified 5 KSHV transcripts de novo comprising 0...
  16. CITED1 nuclear localization and its role in Wilms'tumor pathogenesis
    HAROLD NEWTON LOVVORN; Fiscal Year: 2012
    ..to an In vivo heterotransplant model and will be validated further using an exciting and novel Wilms'tumor cell line that Dr. Loworn and an NCi collaborator have established during this phase. Dr...
  17. Inhibitors of serine biosynthesis
    David M Sabatini; Fiscal Year: 2013
    ..is assessment of the biological activity of these hits by determining their selective cytotoxicity towards a tumor cell line that overexpresses PHGDH and has high flux through the serine biosynthesis pathway, while sparing a tumor ..
  18. NOVEL APPROACHES TO RADIO-IMMUNOCONJUGATE DOSIMETRY
    Lee Chin; Fiscal Year: 1991
    ..Dosimetry will be performed on tissue samples taken from congenic mice inoculated with the Thy 1.2+ murine tumor cell line EL-4 and subsequently treated with anti-Thy1...
  19. Optimization of dipeptide-linked benzimidazole topoisomerase 1 poisons
    Craig Beeson; Fiscal Year: 2009
    ..replacement of the Hoechst with a phenyl- substituted monobenzimidazole provided the agent MB1 that exhibits tumor cell line killing activity similar to LL217 but with substantially reduced off-target effects...
  20. NEURAL CONTROL OF LARGE INTESTINAL MUCOSA
    Helen Cooke; Fiscal Year: 2005
    ..pig, human or nucleotide- receptor deficient mice n response to mechanical stimulation or in a carcinoid tumor cell line, BON...
  21. PARATHYROID HORMONE REGULATION OF OSTEOBLASTIC MRNA
    Nicola Partridge; Fiscal Year: 1993
    ..In previous and preliminary studies, we have confirmed this observation using the osteoblastic tumor cell line, UMR 106-01...
  22. Enhanced Phototoxicity Assay in Reconstituted Skin
    GEORGE DEGEORGE; Fiscal Year: 2002
    ..via the culture media; 3) the human primary keratinocyte-based tissues are a more relevant model than a mouse tumor cell line. In the EPARS, the test substance is applied topically to the reconstructed human skin models, with and ..
  23. DIFFERENTIATION AND STROMA-INDUCTION IN NEURAL TUMORS
    LUCIEN RUBINSTEIN; Fiscal Year: 1980
    ..experimental rat tumors obtained following transplacental ethylnitrosourea (ENU); an established glial rat tumor cell line (C-6); and the neuroepithelial component of a transplantable mouse testicular teratoma (OTT-6050) maintained ..
  24. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 2002
    ..and extended by studies on endogenous AR in a hormone responsive highly differentiated Leydig cell tumor cell line, MA10 cells, that contain a high level of AR...
  25. CLA as a Mediator of Human Prostate Tumor Metastasis
    CHARLES DIMITROFF; Fiscal Year: 2005
    ..We have obtained preliminary evidence showing that a human prostate tumor cell line derived from bone metastases expresses the leukocyte E-selectin ligand, cutaneous lymphocyte-associated ..
  26. GENETIC SUSCEPTIBILITY TO VINYL CHLORIDE INDUCED CANCER
    Roland Valdes; Fiscal Year: 2000
    ..of subjects exposed to VC, liver tissue samples from subjects with ASL, a well characterized VC-associated tumor cell line, the causative agent (VC and its metabolite), and the likelihood of genetic susceptibility...
  27. Bone-Homing Molecules on Human Prostate Tumor Cells
    CHARLES DIMITROFF; Fiscal Year: 2004
    ..We have preliminary evidence showing that a human bone-metastatic prostate tumor cell line expresses a known leukocyte E-selectin ligand, but there is no definitive evidence on its function on these ..
  28. A Novel HTS Cell-Based Assay For Cyclic GMP
    Gary Piazza; Fiscal Year: 2007
    ..The specific aims of this proposal are to: 1) generate a stable transfected colon tumor cell line that expresses the cGMP biosensor, 2) develop an image-based assay to measure cGMP levels in transfected cell ..
  29. Biobehavioral-Cytokine Interactions in Ovarian Cancer
    Susan Lutgendorf; Fiscal Year: 2007
    ..we have observed that stress hormones, such as norepinephrine, induce production of VEGF from an ovarian tumor cell line and that these tumor cells also express beta-adrenergic receptors...
  30. CYLIC AMP REGULATION OF PROTEIN SERETION
    MICHAEL UHLER; Fiscal Year: 1991
    ..An anterior pituitary tumor cell line, designated AtT-20, has been characterized that mimics the cAMP-mediated regulation of POMC-derived peptide ..
  31. BENEFICIAL EFFECTS OF GINKGO BILOBA IN CANCER
    Vassilios Papadopoulos; Fiscal Year: 2002
    ..Ginkgo biloba leaves (EGB 761) inhibited PBR expression and cell proliferation in the aggressive human breast tumor cell line MDA-23 1, but not in a non-aggressive cell line...
  32. Analysis of Loss of Function of MAD2 in Mammals
    Loren Michel; Fiscal Year: 2006
    ..techniques to inactivate one MAD2 allele and induce aneuploidy in an otherwise chromosomally stable human tumor cell line. The metastatic behavior of these cells and their sensitivity to clinically relevant spindle inhibitors will ..
  33. ISOLATION OF A SQUAMOUS CARCINOMA TUMOR SUPPRESSOR GENE
    Bernard Weissman; Fiscal Year: 1999
    ..Demonstration of tumor suppression by transferring a single normal human chromosome into a human tumor cell line maps tumor suppressor gene(s) to that genetic unit...
  34. MELK as Functional Marker for Mammary Tumors
    Alexey Terskikh; Fiscal Year: 2009
    ..MELK expression marks PyMT tumors in vivo and that knocking down MELK expression inhibits growth of a mammary tumor cell line in vitro...
  35. CYTOKINE INDUCTION BY A 48 KD MYCOPLASMA GENE PRODUCT
    Robert Hall; Fiscal Year: 1999
    ..In the process of examining tumor cell line conditioned media for cytokines and factors with differentiation activity, several years ago we identified, ..
  36. Phototoxicity Screening Assay in Reconstituted Skin
    GEORGE DEGEORGE; Fiscal Year: 2006
    ..are composed of primary human keratinocytes in a 3D culture system, a more relevant model than a mouse tumor cell line; and (3) test substances can be applied directly, avoiding the often problematic solubilization of ..
  37. TELOMERIC REPEATS AND THE RESPONSE TO IONIZING RADIATION
    John Murnane; Fiscal Year: 2004
    ..The analysis of spontaneous HSV- tk mutants in the EJ-30 tumor cell line have demonstrated that telomere loss can result in either terminal deletions involving chromosome healing or ..
  38. AGE DEPENDENT LIVER TUMOR SUPPRESSION IN THE LIVER
    Gary Smith; Fiscal Year: 1999
    ..The tumor cell line that is suppressed in the livers of young adult males forms undifferentiated carcinomas in the livers of 100% ..
  39. Molecular Mechanisms of Phenolic Agent-induced Apoptosis
    Ah Ng Kong; Fiscal Year: 2006
    ..whether the activation of the MAPK (ERK, JNK, and p38) pathway by EGCG and other tea polyphenols in two human tumor cell line models (cervical squamous carcinoma HeLa, and colon HT29) is involved in apoptosis...
  40. Molecular Beacon Aptamer for Diagnostic Cancer Imaging
    Weihong Tan; Fiscal Year: 2003
    ..The new imaging and sensing techniques will then be applied for tumor cell line and tissue sample study...
  41. TUMOR CELL LINE PROLIFERATION, MOTILITY AND INVASIVENESS
    DAVID RIESE; Fiscal Year: 2000
    ..Our fifth specific aim is to assess the effects of these hormones on breast tumor cell line proliferation, motility, and invasiveness...
  42. INSTABILITY OF MICROSATELLITES IN MAMMALIAN CELLS
    ROSANN FARBER; Fiscal Year: 2004
    ..results in elevation of mutation rates in the fibroblasts; these rates will be compared with those in a tumor cell line with a mutation in the same gene...
  43. Validation of Galectin-3 as a Target in Cancer Therapy
    Fu Tong Liu; Fiscal Year: 2003
    ..We have demonstrated galectin-3's involvement in promoting proliferation of a leukemic tumor cell line under suboptimal growth conditions, and in inhibition of apoptosis...
  44. GENE THERAPY FOR PROSTATE CANCER
    Selvarangan Ponnazhagan; Fiscal Year: 2007
    ..angiostatin, endostatin and sFlt-1 have shown protection against the growth of a human epithelial ovarian tumor cell line in athymic mice indicating the efficacy of AAV-mediated anti-angiogenic gene therapy...
  45. HUMAN TUMOR-SPECIFIC ANTIBODIES PRODUCED IN SCID MICE
    Denise Shaw; Fiscal Year: 1992
    ..with either purified breast carcinoma TAa glycoproteins (TAG12 or HER-2/neu) or an appropriate human tumor cell line (TAG12-positive T47-D and HER-2/neu-positive SK-BR-3 cells for breast carcinoma or the U-251MG glioma line ..
  46. MELANOMA ANTIGENS INDENTIFIED FROM GMCSF VACCINATION
    FRANK HODI; Fiscal Year: 2002
    ..from initial screening with autologous sera of a cDNA expression library constructed from a patient's tumor cell line. The first revealed a novel product with structural similarities to MUC-1, a member of a family of ..
  47. CD23--AUTOCRINE GROWTH FACTOR AND RECEPTOR
    Wayne Marasco; Fiscal Year: 1991
    ..HIV, EBV infection or EBNA-2 expression on induction of CD23 expression in an EBV-negative continuous Burkitt tumor cell line (Louckes). S1 nuclease protection studies will show which form(s) of CD23 (A or B) mRNA is induced...
  48. THE ROLE OF ELASTIN IN THE PATHOGENESIS OF HYPERTENSION
    Charles Boyd; Fiscal Year: 1993
    ..a) We will synthesize complementary DNA (cDNA) to Type IV collagen-specific mRNA isolated from a human tumor cell line (American Type Culture Collection, HT-1080 Cells). The cDNA will be inserted into pBR322 and grown in E...
  49. Transcriptional Regulation in hMLH1-Silenced Colon Cells
    W Sedwick; Fiscal Year: 2007
    ..Detailed microarray analyses in our laboratory of a human colon tumor cell line in which the mismatch repair gene, hMLH1 is aberrantly silenced for expression have defined a set of genes on ..
  50. MAGNETIC RESONANCE SPECTROSCOPY--CLINICAL IMMUNOTHERAPY
    Brian Ross; Fiscal Year: 1991
    ..The same assay distinguishes a target tumor cell line (K562) from lymphocytes, and demonstrates reproducible changes when that target cell is "killed"...
  51. BIOSYNTHESIS AND FUNCTIONS OF ANIMAL CELL PHOSPHOLIPIDS
    RAPHAEL ZOELLER; Fiscal Year: 1992
    ..These studies will yield better understanding of the regulatory aspects of phospholipid synthesis and the role of a specific class of lipid, ether lipids, in cellular function...
  52. MITOMYCIN C-DNA ADDUCTS IN TUMOR CELLS
    Maria Tomasz; Fiscal Year: 1999
    ..All of the variables will be studies in the EMT66 mouse mammary tumor cell line. Complete MC-DNA adduct patterns formed in the cells will be determined using [3H]-labeled MC and analyzing ..
  53. Permeability of heteromeric heart gap junction channels
    Alonso Moreno; Fiscal Year: 2007
    ..Our model will be a cultured tumor cell line whose membrane potential and growth depends upon direct communication through gap junctions...
  54. HUMAN MONOCLONAL ANTIBODIES PRODUCED IN SCID MICE
    RAYMOND KELLEHER; Fiscal Year: 1990
    ..as a model protein antigen, the haptens dinitrophenol and phthalate conjugated to KLH, and the human lung tumor cell line A549...
  55. GM - CSF - producing tumor cell line as a CML vaccine
    Catherine Wu; Fiscal Year: 2006
    ..The goal of this proposal is to carry out an initial phase I trial using this potentially valuable reagent and thus provide a basis for the continuation and refinement of clinical trials using this reagent. ..
  56. Bcl-2 selective inhibitors: development and application to cancer treatment
    Chengguo Xing; Fiscal Year: 2009
    ..Second, a Bcl-2 inhibitor identified by Wang et al (YC 137) selectively induces apoptosis in a tumor cell line that overexpresses Bcl-2 protein and shows less toxicity to normal cells...
  57. Regulation by kruppel like factor in the colon
    Chi Chuan Tseng; Fiscal Year: 2005
    ..is reduced in colon cancer tissue and that constitutive expression of an antisense GKLF DNA in a colon tumor cell line results in cell hyperproliferation...
  58. FIBROBLAST TUMOR CELL INTERACTIONS IN TUMOR INVASION
    CHITRA BISWAS; Fiscal Year: 1992
    ..Subsequently, using a human tumor cell line LX-1, monoclonal antibodies were raised against TCSF and the factor was purified. TCSF has a mol wt...
  59. Novel Immunoadhesin Molecule for Cancer Therapy
    JAMES LARRICK; Fiscal Year: 2007
    ..Moreover, Met-Fc inhibits growth of a glioma tumor cell line in a mouse xenograft model...
  60. CITED1 nuclear localization and its role in Wilms' tumor pathogenesis
    Harold Lovvorn; Fiscal Year: 2009
    ..lines that misexpress either a predominantly cytosolic or nuclear fraction of CITED1 in the human Wilms'tumor cell line, WIT49, and will assess the effects of these manipulations on Wilms'tumor biology. In his second aim, Dr...
  61. DEVELOPMENT OF ANTIMETASTATIC THERAPY AND MODELS
    Ralph Bernacki; Fiscal Year: 1992
    ..a human ovarian tumor cell line established on ECM from a lymph node metastases has been observed to avidly degrade ECM in vitro...
  62. Mechanisms in Wilms Tumorigenesis
    Bryan Williams; Fiscal Year: 2003
    ..In aim three we will modulate WT1 and p53 expression in a Wilms tumor cell line and determine effects on transcript profiles...