nih 3t3 cells

Summary

Summary: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)

Top Publications

  1. pmc Loss of the RhoGAP SRGP-1 promotes the clearance of dead and injured cells in Caenorhabditis elegans
    Lukas J Neukomm
    Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
    Nat Cell Biol 13:79-86. 2011
  2. ncbi Patched1 regulates hedgehog signaling at the primary cilium
    Rajat Rohatgi
    Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 317:372-6. 2007
  3. ncbi Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells
    Emi Nagoshi
    Department of Molecular Biology and NCCR Frontiers in Genetics, Sciences III, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    Cell 119:693-705. 2004
  4. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
  5. pmc Targeting mitochondrial glutaminase activity inhibits oncogenic transformation
    Jian Bin Wang
    Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA
    Cancer Cell 18:207-19. 2010
  6. pmc Conserved principles of mammalian transcriptional regulation revealed by RNA half-life
    Caroline C Friedel
    Institute for Informatics, Ludwig Maximilians Universitat Munchen, Munich 80333, Germany
    Nucleic Acids Res 37:e115. 2009
  7. pmc High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay
    Lars Dölken
    Max von Pettenkofer Institute, Ludwig Maximilians University Munich, Munich 80337, Germany
    RNA 14:1959-72. 2008
  8. pmc Genome-wide screen reveals APC-associated RNAs enriched in cell protrusions
    Stavroula Mili
    Department of Microbiology, Center for Cell Signaling, University of Virginia, HSC, Charlottesville, Virginia 22908 0577, USA
    Nature 453:115-9. 2008
  9. pmc FoxM1, a critical regulator of oxidative stress during oncogenesis
    Hyun Jung Park
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA
    EMBO J 28:2908-18. 2009
  10. pmc p53 can repress transcription of cell cycle genes through a p21(WAF1/CIP1)-dependent switch from MMB to DREAM protein complex binding at CHR promoter elements
    Marianne Quaas
    Department of Molecular Oncology, Medical School, University of Leipzig, Leipzig, Germany
    Cell Cycle 11:4661-72. 2012

Research Grants

  1. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
  2. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013
  3. ALCOHOL AND CELL ADHESION
    MICHAEL EDWARD CHARNESS; Fiscal Year: 2013
  4. Lysyl Oxidase Propeptide: Breast Cancer Inhibitor
    Gail E Sonenshein; Fiscal Year: 2013
  5. Metabolic regulators in the mechanisms of caloric restriction
    Rozalyn M Anderson; Fiscal Year: 2013
  6. Building and Validating Location Proteomics Databases
    Robert F Murphy; Fiscal Year: 2011
  7. ALCOHOL INTERACTION WITH L1 CELL ADHESION MOLECULE
    MICHAEL CHARNESS; Fiscal Year: 1999
  8. MACROLIDE TOTAL SYNTHESIS
    AMOS SMITH; Fiscal Year: 1990
  9. Cytoplasmic Reactivation of p53 Family Members
    Charles Walker; Fiscal Year: 2004
  10. METABLASTIN AND THE CONTROL OF CELL DIVISION
    ULRICH SCHUBART; Fiscal Year: 1999

Detail Information

Publications301 found, 100 shown here

  1. pmc Loss of the RhoGAP SRGP-1 promotes the clearance of dead and injured cells in Caenorhabditis elegans
    Lukas J Neukomm
    Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
    Nat Cell Biol 13:79-86. 2011
    ..We propose that C. elegans uses the engulfment machinery as part of a primitive, but evolutionarily conserved, survey mechanism that identifies and removes unfit cells within a tissue...
  2. ncbi Patched1 regulates hedgehog signaling at the primary cilium
    Rajat Rohatgi
    Departments of Developmental Biology, Genetics, and Bioengineering and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
    Science 317:372-6. 2007
    ..When Shh bound to Ptc1, Ptc1 left the cilia, leading to accumulation of Smo and activation of signaling. Thus, primary cilia sense Shh and transduce signals that play critical roles in development, carcinogenesis, and stem cell function...
  3. ncbi Circadian gene expression in individual fibroblasts: cell-autonomous and self-sustained oscillators pass time to daughter cells
    Emi Nagoshi
    Department of Molecular Biology and NCCR Frontiers in Genetics, Sciences III, University of Geneva, 30, Quai Ernest Ansermet, CH 1211 Geneva 4, Switzerland
    Cell 119:693-705. 2004
    ..Specifically, the circadian oscillator gates cytokinesis to defined time windows, and mitosis elicits phase shifts in circadian cycles...
  4. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..These results support the hypothesis that mechanical factors impact different cell types in fundamentally different ways, and can trigger specific changes similar to those stimulated by soluble ligands...
  5. pmc Targeting mitochondrial glutaminase activity inhibits oncogenic transformation
    Jian Bin Wang
    Department of Molecular Medicine, Cornell University, Ithaca, NY 14853, USA
    Cancer Cell 18:207-19. 2010
    ..These findings highlight a previously unappreciated connection between Rho GTPase activation and cellular metabolism and demonstrate that targeting glutaminase activity can inhibit oncogenic transformation...
  6. pmc Conserved principles of mammalian transcriptional regulation revealed by RNA half-life
    Caroline C Friedel
    Institute for Informatics, Ludwig Maximilians Universitat Munchen, Munich 80333, Germany
    Nucleic Acids Res 37:e115. 2009
    ..Therefore, this atlas of transcript half-lives provides new fundamental insights into many cellular processes...
  7. pmc High-resolution gene expression profiling for simultaneous kinetic parameter analysis of RNA synthesis and decay
    Lars Dölken
    Max von Pettenkofer Institute, Ludwig Maximilians University Munich, Munich 80337, Germany
    RNA 14:1959-72. 2008
    ....
  8. pmc Genome-wide screen reveals APC-associated RNAs enriched in cell protrusions
    Stavroula Mili
    Department of Microbiology, Center for Cell Signaling, University of Virginia, HSC, Charlottesville, Virginia 22908 0577, USA
    Nature 453:115-9. 2008
    ..APC is required for the accumulation of transcripts in protrusions. Our results suggest a new type of RNA anchoring mechanism as well as a new, unanticipated function for APC in localizing RNAs...
  9. pmc FoxM1, a critical regulator of oxidative stress during oncogenesis
    Hyun Jung Park
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA
    EMBO J 28:2908-18. 2009
    ..Together, our results identify FoxM1 as a key regulator of ROS in dividing cells, and provide insights into the mechanism how tumour cells use FoxM1 to control oxidative stress to escape premature senescence and apoptosis...
  10. pmc p53 can repress transcription of cell cycle genes through a p21(WAF1/CIP1)-dependent switch from MMB to DREAM protein complex binding at CHR promoter elements
    Marianne Quaas
    Department of Molecular Oncology, Medical School, University of Leipzig, Leipzig, Germany
    Cell Cycle 11:4661-72. 2012
    ..In the case of promoters that do not hold CDE or E2F elements, binding of DREAM and MMB solely relies on a CHR site. Thus, p53 can repress target genes indirectly through CHR elements...
  11. ncbi EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration
    Ying Wen
    Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Nat Cell Biol 6:820-30. 2004
    ..These results show an evolutionarily conserved pathway for microtubule capture, and suggest that mDia functions as a scaffold protein for EB1 and APC to stabilize microtubules and promote cell migration...
  12. pmc The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins
    Eric W Humke
    Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    Genes Dev 24:670-82. 2010
    ..We propose that the Sufu-Gli3 interaction is a major control point in the Hedgehog pathway, a pathway that plays important roles in both development and cancer...
  13. pmc Mouse centric and pericentric satellite repeats form distinct functional heterochromatin
    Mounia Guenatri
    Institut Curie Research section, UMR218 du Centre National pour la Recherche Scientifique, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    J Cell Biol 166:493-505. 2004
    ..Thus, we define functionally independent centromeric subdomains, which spatio-temporal isolation is proposed to be important for centromeric cohesion and dissociation during chromosome segregation...
  14. pmc An inducible translocation strategy to rapidly activate and inhibit small GTPase signaling pathways
    Takanari Inoue
    Stanford University, Department of Molecular Pharmacology, Clark Center, 318 Campus Drive, Stanford, California 94305 5439, USA
    Nat Methods 2:415-8. 2005
    ..As signaling processes often occur within minutes, such rapid perturbations provide a powerful tool to investigate the role, selectivity and timing of Rho GTPase-mediated signaling processes...
  15. ncbi Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation
    Gianluca Canettieri
    Department of Experimental Medicine, Sapienza University, 324 Viale Regina Elena, 00161 Rome, Italy
    Nat Cell Biol 12:132-42. 2010
    ..These data identify an integrated HDAC- and ubiquitin-mediated circuitry, where acetylation of Gli proteins functions as an unexpected key transcriptional checkpoint of Hedgehog signalling...
  16. ncbi Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase
    Ellen J Ezratty
    Department of Anatomy and Cell Biology, Columbia University, 630 West 168th Street, BB 1217, New York, NY 10032, USA
    Nat Cell Biol 7:581-90. 2005
    ..Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation...
  17. ncbi Cep97 and CP110 suppress a cilia assembly program
    Alexander Spektor
    Department of Pathology and NYU Cancer Institute, New York University School of Medicine, Smilow Research Center, 522 First Avenue, New York, NY 10016, USA
    Cell 130:678-90. 2007
    ..Identification of Cep97 and other genes involved in regulation of cilia assembly may accelerate our understanding of human ciliary diseases, including renal disease and retinal degeneration...
  18. pmc The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles
    Giulia Guarguaglini
    Department of Cell Biology, Max Planck Institute for Biochemistry, D 82152, Martinsried, Germany
    Mol Biol Cell 16:1095-107. 2005
    ..We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division...
  19. pmc SUN1 interacts with nuclear lamin A and cytoplasmic nesprins to provide a physical connection between the nuclear lamina and the cytoskeleton
    Farhana Haque
    Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Mol Cell Biol 26:3738-51. 2006
    ..Our data provide evidence of a physical nuclear-cytoskeletal connection that is likely to be a key mechanism in nuclear-cytoplasmic communication and regulation of nuclear position...
  20. pmc Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium
    Ljiljana Milenkovic
    Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Cell Biol 187:365-74. 2009
    ..We anticipate that this alternative route will be used by other signaling proteins that function at cilia. The path taken by Smo may allow novel strategies for modulation of Hh signaling in cancer and regeneration...
  21. ncbi Mip/LIN-9 regulates the expression of B-Myb and the induction of cyclin A, cyclin B, and CDK1
    Mark Pilkinton
    Department of Pharmacology, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 282:168-75. 2007
    ..These data support the concept that Mip/LIN-9 is required for the expression of B-Myb, and both proteins collaborate in the control of the cell cycle progression via the regulation of S phase and mitotic cyclins...
  22. pmc Cytoskeletal coherence requires myosin-IIA contractility
    Yunfei Cai
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Cell Sci 123:413-23. 2010
    ..We suggest that NMIIA creates a coherent actin network through the formation of circumferential actin bundles that mechanically link elements of the peripheral actin cytoskeleton where much of the force is generated during spreading...
  23. pmc Caveolin-1 regulates cell polarization and directional migration through Src kinase and Rho GTPases
    Araceli Grande-García
    Integrin Signaling Laboratory, Department of Vascular Biology and Inflammation, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
    J Cell Biol 177:683-94. 2007
    ..These findings highlight the importance of caveolin-1 in the establishment of cell polarity during directional migration through coordination of the signaling of Src kinase and Rho GTPases...
  24. pmc A voltage-driven switch for ion-independent signaling by ether-à-go-go K+ channels
    Andrew P Hegle
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109 1048, USA
    Proc Natl Acad Sci U S A 103:2886-91. 2006
    ....
  25. pmc DYRK1A protein kinase promotes quiescence and senescence through DREAM complex assembly
    Larisa Litovchick
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Genes Dev 25:801-13. 2011
    ..These data reveal an important role for DYRK1A in the regulation of DREAM activity and entry into quiescence...
  26. ncbi Concerted action of poly(A) nucleases and decapping enzyme in mammalian mRNA turnover
    Akio Yamashita
    Department of Biochemistry and Molecular Biology, The University of Texas Medical School, Houston, Texas 77030, USA
    Nat Struct Mol Biol 12:1054-63. 2005
    ..Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay...
  27. pmc The GTPase-activating protein GRAF1 regulates the CLIC/GEEC endocytic pathway
    Richard Lundmark
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK
    Curr Biol 18:1802-8. 2008
    ....
  28. pmc Phosphorylation of Skp2 regulated by CDK2 and Cdc14B protects it from degradation by APC(Cdh1) in G1 phase
    Geneviève Rodier
    Institut de Recherche en Immunologie et Cancérologie, Universite de Montreal, Montreal, Quebec, Canada
    EMBO J 27:679-91. 2008
    ..Thus, our results reveal that reversible phosphorylation plays a key role in the timing of Skp2 expression in the cell cycle...
  29. pmc Initiation of attachment and generation of mature focal adhesions by integrin-containing filopodia in cell spreading
    Michael A Partridge
    Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    Mol Biol Cell 17:4237-48. 2006
    ..Finally, the nascent adhesions coalesced until they formed mature FAs...
  30. pmc hnRNP Q and PTB modulate the circadian oscillation of mouse Rev-erb alpha via IRES-mediated translation
    Do Yeon Kim
    Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Gyeong buk, 790 784, Republic of Korea
    Nucleic Acids Res 38:7068-78. 2010
    ..We suggest that the rhythmic binding affinity of hnRNP Q to the Rev-erb α IRES and the change in PTB cytosolic levels lead to maintenance of the oscillation profile of the Rev-erb α protein...
  31. pmc The CHR promoter element controls cell cycle-dependent gene transcription and binds the DREAM and MMB complexes
    Gerd A Müller
    Molecular Oncology, Medical School, University of Leipzig, Semmelweisstrasse 14, 04103 Leipzig, Germany
    Nucleic Acids Res 40:1561-78. 2012
    ..Our findings indicate that the CHR is central to DREAM/MMB-dependent transcriptional control during the cell cycle...
  32. pmc DISC1 regulates primary cilia that display specific dopamine receptors
    Aaron Marley
    Department of Psychiatry, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 5:e10902. 2010
    ..We identified a role of DISC1 in the cell biology of primary cilia that display disease-relevant dopamine receptors...
  33. pmc Gli2 trafficking links Hedgehog-dependent activation of Smoothened in the primary cilium to transcriptional activation in the nucleus
    Jynho Kim
    Department of Developmental Biology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:21666-71. 2009
    ..Gli2 thus functions as a dynamic monitor of Smo activity in the cilium and thereby links Hh pathway activation in the cilium to transcriptional activation in the nucleus...
  34. pmc LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1
    Shihoko Kojima
    Laboratory of Chronogenomics, Mitsubishi Kagaku Institute of Life Sciences, Minamiooya, Machida, Tokyo, Japan
    Proc Natl Acad Sci U S A 104:1859-64. 2007
    ..These data indicate that mLARKs are novel posttranscriptional regulators of mammalian circadian clocks...
  35. ncbi Immunoaffinity profiling of tyrosine phosphorylation in cancer cells
    John Rush
    Cell Signaling Technology Inc, 166B Cummings Center, Beverly, Massachusetts 01915, USA
    Nat Biotechnol 23:94-101. 2005
    ....
  36. ncbi Hedgehog regulates smoothened activity by inducing a conformational switch
    Yun Zhao
    Department of Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Nature 450:252-8. 2007
    ..Hence, by employing multiple Arg clusters as inhibitory elements counteracted by differential phosphorylation, SMO acts as a rheostat to translate graded HH signals into distinct responses...
  37. pmc Phospho-caveolin-1 mediates integrin-regulated membrane domain internalization
    Miguel A Del Pozo
    Centro Nacional de Investigaciones Cardiovasculares CNIC, Madrid 28029, Spain
    Nat Cell Biol 7:901-8. 2005
    ..These data define a novel molecular mechanism for growth and tumour suppression by caveolin-1...
  38. ncbi The EWS/FLI1 oncogenic transcription factor deregulates GLI1
    J P Zwerner
    Department of Dermatology, Stanford University School of Medicine, Palo Alto, CA, USA
    Oncogene 27:3282-91. 2008
    ..Inhibition of GLI1 expression via RNAi results in reduced anchorage-independent growth in an EFT cell line. GLI1 appears to be a transcriptionally deregulated target of EWS/FLI1 that mediates a portion of its tumorigenic phenotype...
  39. ncbi Inhibition of dynamin mediated endocytosis by the dynoles--synthesis and functional activity of a family of indoles
    Timothy A Hill
    Chemistry, School of Environmental and Life Sciences, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia
    J Med Chem 52:3762-73. 2009
    ..The dynoles represent a new series of tools to better probe endocytosis and dynamin-mediated trafficking events in a variety of cells...
  40. pmc Nuclear phosphoinositide 3-kinase beta controls double-strand break DNA repair
    Amit Kumar
    Department of Immunology and Oncology, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Madrid E 28049, Spain
    Proc Natl Acad Sci U S A 107:7491-6. 2010
    ..These results point at class I PI3Kbeta as a critical sensor of genomic integrity...
  41. pmc Dual modification of BMAL1 by SUMO2/3 and ubiquitin promotes circadian activation of the CLOCK/BMAL1 complex
    Jiwon Lee
    School of Biological Sciences, Seoul National University, Seoul 151 742, Republic of Korea
    Mol Cell Biol 28:6056-65. 2008
    ..These results indicate that dual modification of BMAL1 by SUMO2/3 and ubiquitin is essential for circadian activation and degradation of the CLOCK/BMAL1 complex...
  42. pmc Phosphorylation of the cool-1/beta-Pix protein serves as a regulatory signal for the migration and invasive activity of Src-transformed cells
    Qiyu Feng
    Department of Molecular Medicine, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 285:18806-16. 2010
    ....
  43. ncbi The human cytomegalovirus-encoded chemokine receptor US28 promotes angiogenesis and tumor formation via cyclooxygenase-2
    David Maussang
    Leiden Amsterdam Center for Drug Research, Division of Medicinal Chemistry, Faculty of Sciences, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
    Cancer Res 69:2861-9. 2009
    ..Hence, the development of HCMV-related proliferative diseases may partially be ascribed to the ability of US28 to activate COX-2...
  44. ncbi K-ras codon-specific mutations produce distinctive metabolic phenotypes in NIH3T3 mice [corrected] fibroblasts
    Pedro Vizan
    Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, Spain
    Cancer Res 65:5512-5. 2005
    ....
  45. pmc A chemical screen identifies class a g-protein coupled receptors as regulators of cilia
    Prachee Avasthi
    Department of Biochemistry, University of California, San Francisco, CA 94158, USA
    ACS Chem Biol 7:911-9. 2012
    ....
  46. ncbi Responsive small molecular hydrogels based on adamantane-peptides for cell culture
    Cuihong Yang
    State Key Laboratory of Medicinal Chemical Biology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People s Republic of China
    J Phys Chem B 116:633-8. 2012
    ..The results shown in this study indicate that small molecular hydrogels based on adamantane-peptides have great potentials in research fields where further analysis of cells is needed...
  47. ncbi RHAMM is differentially expressed in the cell cycle and downregulated by the tumor suppressor p53
    Sindy Sohr
    Molecular Oncology, Department of Obstetrics and Gynecology, University of Leipzig, Leipzig, Germany
    Cell Cycle 7:3448-60. 2008
    ..Additionally, p53-dependent downregulation is consistent with an oncogenic function of RHAMM and the recently reported tumor-suppressive function of CD44 transcriptional repression by p53...
  48. pmc Phosphorylation of fibroblast growth factor (FGF) receptor 1 at Ser777 by p38 mitogen-activated protein kinase regulates translocation of exogenous FGF1 to the cytosol and nucleus
    Vigdis Sørensen
    Centre for Cancer Biomedicine, Faculty Division Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Mol Cell Biol 28:4129-41. 2008
    ..These data demonstrate a crucial role for p38alpha MAPK in the regulated translocation of exogenous FGF1 into the cytosol/nucleus, and they reveal a specific role for p38alpha MAPK-mediated serine phosphorylation of FGFR1...
  49. pmc Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells
    Natalia Shcherbik
    Department of Cell Biology, University of Medicine and Dentistry of New Jersey, 2 Medical Center Drive, Stratford, New Jersey 08084, USA
    EMBO Rep 11:106-11. 2010
    ..The impact of excessive aberrant RNAs on the degradation machinery is an unrecognized mechanism that might contribute to biological properties of ActD...
  50. pmc Reduced expression of the ROCK inhibitor Rnd3 is associated with increased invasiveness and metastatic potential in mesenchymal tumor cells
    Cristina Belgiovine
    Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche CNR, Pavia, Italy
    PLoS ONE 5:e14154. 2010
    ..Cancer cells can switch from one mechanism to the other in response to different stimuli, limiting the efficacy of antimetastatic therapies...
  51. pmc Human immunodeficiency virus type 1 Tat accelerates Kaposi sarcoma-associated herpesvirus Kaposin A-mediated tumorigenesis of transformed fibroblasts in vitro as well as in nude and immunocompetent mice
    Xiuying Chen
    Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, PR China
    Neoplasia 11:1272-84. 2009
    ....
  52. pmc Impact of feedback phosphorylation and Raf heterodimerization on normal and mutant B-Raf signaling
    Daniel A Ritt
    Laboratory of Cell and Developmental Signaling, NCI Frederick, Building 560, Frederick, MD 21702, USA
    Mol Cell Biol 30:806-19. 2010
    ..Finally, we find that B-Raf and C-Raf proteins containing mutations identified in certain developmental disorders constitutively heterodimerize and that their signaling activity can also be modulated by feedback phosphorylation...
  53. ncbi HCMV-encoded chemokine receptor US28 mediates proliferative signaling through the IL-6-STAT3 axis
    Erik Slinger
    Leiden Amsterdam Center for Drug Research, Division of Medicinal Chemistry, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
    Sci Signal 3:ra58. 2010
    ..of vascular endothelial growth factor and interleukin-6 (IL-6) in supernatants of US28-expressing NIH 3T3 cells. Increased IL-6 was associated with increased activation of the signal transducer and activator of ..
  54. ncbi Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport
    Hidetaka Kosako
    Division of Cellular Proteomics BML, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Nat Struct Mol Biol 16:1026-35. 2009
    ..Thus, we propose that ERK phosphorylates various nucleoporins to regulate nucleocytoplasmic transport...
  55. ncbi Statins modulate transcriptional activity of heme-oxygenase-1 promoter in NIH 3T3 Cells
    May F Mrad
    Department of Biochemistry and Molecular Genetics, American University of Beirut, Lebanon
    J Cell Biochem 113:3466-75. 2012
    ..In the present study, we used NIH 3T3 cells which express HO-1 to investigate the molecular mechanisms of HO-1 induction by statins...
  56. pmc The interferon-gamma-induced GTPase, mGBP-2, inhibits tumor necrosis factor alpha (TNF-alpha) induction of matrix metalloproteinase-9 (MMP-9) by inhibiting NF-kappaB and Rac protein
    Sujata Balasubramanian
    Department of Biological Sciences, University of Toledo, Toledo, Ohio 43606, USA
    J Biol Chem 286:20054-64. 2011
    ..In addition, TNF-α activation of NF-κB in NIH 3T3 cells is dependent on Rac activation, as evidenced by the inhibition of TNF-α induction of NF-κB-mediated ..
  57. ncbi Cell-free nucleic acids circulating in the plasma of colorectal cancer patients induce the oncogenic transformation of susceptible cultured cells
    Dolores C García-Olmo
    Experimental Research Unit, General University Hospital of Albacete, Albacete, Spain
    Cancer Res 70:560-7. 2010
    ..4 mum. Our results indicate that plasma from cancer patients is able to transform cultured cells oncogenically, supporting the previously proposed hypothesis of genometastasis...
  58. ncbi The human formin FHOD1 contains a bipartite structure of FH3 and GTPase-binding domains required for activation
    Antje Schulte
    Abteilung Physikalische Biochemie, Max Planck Institut fur molekulare Physiologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    Structure 16:1313-23. 2008
    ..Mutation of one residue in the predicted DAD-interaction surface efficiently activates FHOD1 in cells. These results demonstrate that DRFs have evolved different molecular solutions to govern their autoregulation and GTPase specificity...
  59. pmc SUMOylation by Pias1 regulates the activity of the Hedgehog dependent Gli transcription factors
    Barny Cox
    Developmental Neurobiology, Medical Research Council National Institute for Medical Research, London, United Kingdom
    PLoS ONE 5:e11996. 2010
    ..Whether other post-translational modifications contribute to the regulation of Gli protein activity has been unclear...
  60. pmc A CHOP-regulated microRNA controls rhodopsin expression
    Shannon Behrman
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA
    J Cell Biol 192:919-27. 2011
    ....
  61. pmc Fibroblast-derived 3D matrix differentially regulates the growth and drug-responsiveness of human cancer cells
    Ilya Serebriiskii
    Division of Basic Science Tumor Cell Biology, Fox Chase Cancer Center, Philadelphia, PA 19111, United States
    Matrix Biol 27:573-85. 2008
    ..Based on these results, we propose that these matrices potentially have value as in vitro drug screening platforms...
  62. ncbi Scyphozoan jellyfish venom metalloproteinases and their role in the cytotoxicity
    Hyunkyoung Lee
    Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 660 701, South Korea
    Toxicon 58:277-84. 2011
    ..of these venom proteases was examined by comparing their proteolytic activity and the cytotoxicity in NIH 3T3 cells. The relative cytotoxic potency was C. nozakii > N. nomurai > A. aurita > R. esculenta...
  63. pmc Differential phosphoproteomics of fibroblast growth factor signaling: identification of Src family kinase-mediated phosphorylation events
    Debbie L Cunningham
    School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
    J Proteome Res 9:2317-28. 2010
    ..We further identify 18 SFK-dependent Dok1 interactions and 9 SFK-dependent Dok1 phosphorylation sites, 6 of which had not previously been known to be SFK-dependent...
  64. ncbi Deoxyribonucleic acid methylation controls cell type-specific expression of steroidogenic factor 1
    Erling A Hoivik
    Department of Biomedicine, University of Bergen, Bergen, Norway
    Endocrinology 149:5599-609. 2008
    ..In conclusion, we identified a region within the SF1/Sf1 gene that epigenetically directs cell-specific expression of SF1...
  65. ncbi Cell-penetrating metal complex optical probes: targeted and responsive systems based on lanthanide luminescence
    Craig P Montgomery
    Department of Chemistry, Durham University, Durham DH1 3LE, U K
    Acc Chem Res 42:925-37. 2009
    ....
  66. ncbi Oxidation of DJ-1-dependent cell transformation through direct binding of DJ-1 to PTEN
    Yun chul Kim
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Int J Oncol 35:1331-41. 2009
    ..These results suggest that the oxidative status of DJ-1 regulates PTEN activity, leading to cell proliferation and transformation...
  67. pmc piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling
    Huiyan Huang
    Department of Pharmacology, Center for Developmental Genetics, Stony Brook University, Stony Brook, NY 11794 5140, USA
    Dev Cell 20:376-87. 2011
    ..We propose that mitochondrial-surface PA generated by MitoPLD/Zuc recruits or activates nuage components critical for piRNA production...
  68. ncbi Transcriptional output of the Salvador/warts/hippo pathway is controlled in distinct fashions in Drosophila melanogaster and mammalian cell lines
    Xiaomeng Zhang
    Cell Growth and Proliferation Laboratory and Cell Cycle and Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia
    Cancer Res 69:6033-41. 2009
    ..melanogaster and optimally activate Sd. This shows that Yki/YAP WW domains have distinct regulatory roles in different cell types and implies the existence of proteins that promote tissue growth in collaboration with Yki and Sd...
  69. pmc Transcriptional control of preadipocyte determination by Zfp423
    Rana K Gupta
    Department of Cancer Biology and Division of Metabolism and Chronic Disease, Dana Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 464:619-23. 2010
    ..This study identifies Zfp423 as a transcriptional regulator of preadipocyte determination...
  70. ncbi PAX-FKHR function as pangenes by simultaneously inducing and inhibiting myogenesis
    F Graf Finckenstein
    Department of Pathology, Children s Hospital Los Angeles, Los Angeles, CA 90027, USA
    Oncogene 27:2004-14. 2008
    ..We propose a model whereby PAX-FKHR commit a yet undefined precursor cell to the myogenic lineage while at the same time inhibit terminal differentiation, thereby contributing to ARMS formation...
  71. pmc Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis
    Kevin D Smith
    Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Cell Biol 187:15-23. 2009
    ..Together, these experiments indicate that the Tim-Tipin complex stabilizes replication forks both by preventing the accumulation of ssDNA upstream of ATR-Chk1 function and by facilitating phosphorylation of Chk1 by ATR...
  72. pmc Compartmentalized Ras proteins transform NIH 3T3 cells with different efficiencies
    Chiang Min Cheng
    Department of Molecular and Cellular Biology, Lester and Sue Smith Breast Center, 1 Baylor Plaza, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell Biol 31:983-97. 2011
    ..Surprisingly, PM-restricted oncogenic Ras when expressed alone could only weakly transform NIH 3T3 cells; however, when constitutively active Cdc42 was coexpressed, together they transformed cells much more ..
  73. ncbi Curcumin down-regulates the multidrug-resistance mdr1b gene by inhibiting the PI3K/Akt/NF kappa B pathway
    Byeong Hyeok Choi
    Department of Biomedical Science and Technology, IBST, Konkuk University, Seoul 143 701, Republic of Korea
    Cancer Lett 259:111-8. 2008
    ..Thus, curcumin can contribute to the reversal of the MDR phenotype, probably due to the suppression of P-gp expression via the inhibition of the PI3K/Akt/NF-kappa B signaling pathway...
  74. pmc DJ-1 has a role in antioxidative stress to prevent cell death
    Takahiro Taira
    Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060 0812, Japan
    EMBO Rep 5:213-8. 2004
    ..These results clearly showed that DJ-1 has a role in the antioxidative stress reaction and that mutations of DJ-1 lead to cell death, which is observed in PD...
  75. pmc Lipid-coated nano-calcium-phosphate (LNCP) for gene delivery
    Chenguang Zhou
    Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA
    Int J Pharm 392:201-8. 2010
    ....
  76. pmc The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B
    Marcela Rosas
    Infection, Immunity and Biochemistry, Cardiff University School of Medicine, Cardiff, UK
    J Leukoc Biol 88:169-80. 2010
    ..The definition of the 7/4:Ly-6B antigen will allow further characterization and specific modulation of Ly-6B-expressing cells in vivo...
  77. ncbi Regulation of the inducible nuclear dual-specificity phosphatase DUSP5 by ERK MAPK
    Anna Kucharska
    Cancer Research UK Stress Response Laboratory, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, Scotland, UK
    Cell Signal 21:1794-805. 2009
    ..We conclude that DUSP5 is stabilised by complex formation with its physiological substrate and that this may reinforce its activity as both a phosphatase and nuclear anchor for ERK2...
  78. pmc FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST
    Yanhua Zheng
    Brain Tumor Center and Department of Neuro Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Mol Cell 35:11-25. 2009
    ..These findings uncover the importance of sequential modification of FAK-by serine phosphorylation, isomerization, and tyrosine dephosphorylation--in the regulation of FAK activity and, thereby, in Ras-related tumor metastasis...
  79. pmc WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity
    Andrew Xiao
    Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10065, USA
    Nature 457:57-62. 2009
    ..Our work demonstrates a new mechanism that regulates the DNA damage response and expands our knowledge of domains that contain intrinsic tyrosine kinase activity...
  80. ncbi Behaviors of NIH-3T3 fibroblasts on graphene/carbon nanotubes: proliferation, focal adhesion, and gene transfection studies
    Soo Ryoon Ryoo
    Department of Chemistry, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Korea
    ACS Nano 4:6587-98. 2010
    ..e., gene transfection and expression)...
  81. pmc Linear arrays of nuclear envelope proteins harness retrograde actin flow for nuclear movement
    G W Gant Luxton
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
    Science 329:956-9. 2010
    ....
  82. ncbi The pancreatitis-induced vacuole membrane protein 1 triggers autophagy in mammalian cells
    Alejandro Ropolo
    Department of Physiology, School of Medicine, University of Buenos Aires, and CEMIC University Hospital, 4102 Av E Galvan, Buenos Aires C1431FWO, Argentina
    J Biol Chem 282:37124-33. 2007
    ..Our results identify VMP1 as a novel autophagy-related membrane protein involved in the initial steps of the mammalian cell autophagic process...
  83. ncbi The cold-inducible RNA-binding protein migrates from the nucleus to cytoplasmic stress granules by a methylation-dependent mechanism and acts as a translational repressor
    Frederic De Leeuw
    Laboratoire de Chimie Biologique, Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, 12 rue des Profs Jeener et Brachet, 6041 Gosselies, Belgium
    Exp Cell Res 313:4130-44. 2007
    ..Altogether, our findings further reveal the diversity of mechanisms by which CIRP is regulated by environmental stresses and provide new insights into CIRP cytoplasmic function...
  84. ncbi Hedgehog signaling promotes the degradation of tumor suppressor Sufu through the ubiquitin-proteasome pathway
    S Yue
    Laboratory of Reproductive Medicine, Nanjing Medical University, Jiangsu, PR China
    Oncogene 28:492-9. 2009
    ..These results indicate that Shh signaling regulates Sufu activity by inducing its turnover via the ubiquitin-proteasome system...
  85. pmc Myeloid leukemia factor 1 regulates p53 by suppressing COP1 via COP9 signalosome subunit 3
    Noriko Yoneda-Kato
    Department of Animal Molecular Genetics, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara, Japan
    EMBO J 24:1739-49. 2005
    ..Exogenous expression of COP1 overcomes MLF1-induced growth arrest. These results indicate that MLF1 is a critical regulator of p53 and suggest its involvement in leukemogenesis through a novel CSN3-COP1 pathway...
  86. ncbi A CK2-dependent mechanism for degradation of the PML tumor suppressor
    Pier Paolo Scaglioni
    Cancer Biology and Genetics Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Cell 126:269-83. 2006
    ..These data identify a key posttranslational mechanism that controls PML protein levels and provide therapeutic means toward PML restoration through CK2 inhibition...
  87. ncbi Activation of FOXO1 by Cdk1 in cycling cells and postmitotic neurons
    Zengqiang Yuan
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 319:1665-8. 2008
    ..These findings define a conserved signaling link between Cdk1 and FOXO1 that may have a key role in diverse biological processes, including the degeneration of postmitotic neurons...
  88. pmc The 5' external transcribed spacer in mouse ribosomal RNA contains two cleavage sites
    Tatyana Kent
    Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    RNA 15:14-20. 2009
    ..We discuss the finding of a second processing site in mammalian 5'ETS in relation to the involvement of the U3 snoRNA in pre-rRNA processing and present a revised map of the mouse 18S rRNA processing pathway...
  89. ncbi A direct role for phosphatidylinositol-4,5-bisphosphate in unconventional secretion of fibroblast growth factor 2
    Koen Temmerman
    Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Traffic 9:1204-17. 2008
    ..These data show that a transient interaction with PI(4,5)P(2) associated with the inner leaflet of plasma membranes represents the initial step of the unconventional secretory pathway of FGF-2...
  90. ncbi A functional screen to identify novel effectors of hematopoietic stem cell activity
    Eric Deneault
    Molecular Genetics of Stem Cells Laboratory, Institute of Research in Immunology and Cancer IRIC, University of Montreal, Montreal, Quebec H3C 3J7, Canada
    Cell 137:369-79. 2009
    ..The utilization of this screening method together with the creation of a database enriched for potential determinants of hematopoietic stem cell self-renewal will serve as a resource to uncover regulatory networks in these cells...
  91. pmc Staged stromal extracellular 3D matrices differentially regulate breast cancer cell responses through PI3K and beta1-integrins
    Remedios Castelló-Cros
    Cancer Genetics and Signaling Program, Fox Chase Cancer Center, Philadelphia, PA 19111 2497, USA
    BMC Cancer 9:94. 2009
    ..The goal of this study was to analyze the direct effects of staged stroma-derived extracellular matrices on breast cancer cell behavior...
  92. ncbi Ripples from neighbouring transcription
    Miki Ebisuya
    Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Nat Cell Biol 10:1106-13. 2008
    ..These results suggest that transcriptional activation has a ripple effect, which may be advantageous for coordinated expression...
  93. pmc Eag1 expression interferes with hypoxia homeostasis and induces angiogenesis in tumors
    Bryan R Downie
    Max Planck Institute of Experimental Medicine, Hermann Rein Str 3, 37075 Gottingen, Germany
    J Biol Chem 283:36234-40. 2008
    ..Our data suggest that Eag1 interferes with the cellular mechanism for maintaining oxygen homeostasis, increasing HIF-1 activity, and thereby VEGF secretion and tumor vascularization...
  94. pmc TEAD mediates YAP-dependent gene induction and growth control
    Bin Zhao
    Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 22:1962-71. 2008
    ..Our study reveals TEAD as a new component in the Hippo pathway playing essential roles in mediating biological functions of YAP...
  95. pmc Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade
    Joel M Hyman
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 106:14132-7. 2009
    ..These antagonists therefore constitute a valuable set of chemical tools for interrogating downstream Hh signaling mechanisms and for developing chemotherapies against Hh pathway-related cancers...
  96. ncbi Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive
    Estela Jacinto
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 6:1122-8. 2004
    ..mTORC2 is not upstream of the mTORC1 effector S6K. Thus, two distinct TOR complexes constitute a primordial signalling network conserved in eukaryotic evolution to control the fundamental process of cell growth...
  97. pmc Novel mutant-selective EGFR kinase inhibitors against EGFR T790M
    Wenjun Zhou
    Department of Cancer Biology, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nature 462:1070-4. 2009
    ..Our findings demonstrate that functional pharmacological screens against clinically important mutant kinases represent a powerful strategy to identify new classes of mutant-selective kinase inhibitors...
  98. pmc A two-step, PU.1-dependent mechanism for developmentally regulated chromatin remodeling and transcription of the c-fms gene
    Hanna Krysinska
    University of Leeds, Leeds Institute of Molecular Medicine, St James s University Hospital, Wellcome Trust Brenner Building, Leeds LS9 7TF, United Kingdom
    Mol Cell Biol 27:878-87. 2007
    ..The two-step mechanism of developmental gene activation that we describe here may be utilized to regulate gene activity in a variety of developmental pathways...
  99. pmc Cytomegalovirus M45 cell death suppression requires receptor-interacting protein (RIP) homotypic interaction motif (RHIM)-dependent interaction with RIP1
    Jason W Upton
    Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:16966-70. 2008
    ....
  100. ncbi PDGFRalphaalpha signaling is regulated through the primary cilium in fibroblasts
    Linda Schneider
    Department of Biochemistry, Institute for Molecular Biology and Physiology, University of Copenhagen, The August Krogh Building, Universitetsparken 13, DK 2100 Copenhagen Ø, Denmark
    Curr Biol 15:1861-6. 2005
    ..We propose that ciliary PDGFRalphaalpha signaling is linked to tissue homeostasis and to mitogenic signaling pathways...
  101. ncbi Ribosomal stress induces processing of Mybbp1a and its translocation from the nucleolus to the nucleoplasm
    Tomohiro Yamauchi
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Genes Cells 13:27-39. 2008
    ..These results suggest that Mybbp1a may connect the ribosome biogenesis and the Myb-dependent transcription, which controls cell cycle progression and proliferation...

Research Grants64

  1. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
    ..A number of candidate proteins that may be involved in the PRDM16-driven cell fate transition between myoblasts and brown fat cells were identified. ..
  2. MECHANISMS OF EUKARYOTIC TRANSCRIPTIONAL REGULATION
    Michael R Green; Fiscal Year: 2013
    ..28 factors required for epigenetic silencing of Fas, and a select group of other genes, in ras-transformed NIH 3T3 cells. This model system, in conjunction with the experimental reagents we have developed, provides a unique ..
  3. ALCOHOL AND CELL ADHESION
    MICHAEL EDWARD CHARNESS; Fiscal Year: 2013
    ..These experiments will expand our knowledge of how ethanol disrupts fetal development, why individuals differ in sensitivity to ethanol teratogenesis, and how drugs might be designed to reduce ethanol toxicity. ..
  4. Lysyl Oxidase Propeptide: Breast Cancer Inhibitor
    Gail E Sonenshein; Fiscal Year: 2013
    ..as the "ras recision" gene (rrg) with an ability to inhibit the transforming activity of the Ras oncogene in NIH 3T3 cells. Consistently many cancers display reduced LOX gene expression...
  5. Metabolic regulators in the mechanisms of caloric restriction
    Rozalyn M Anderson; Fiscal Year: 2013
    ..We expect that the significance of this study will extend to the investigation of diseases that are promoted by deregulation of WAT. ..
  6. Building and Validating Location Proteomics Databases
    Robert F Murphy; Fiscal Year: 2011
    ..25,000 independent cell lines expressing GFP protein fusions will be created in NIH 3T3 cells using high-throughput CD-tagging (protein-trapping) methods...
  7. ALCOHOL INTERACTION WITH L1 CELL ADHESION MOLECULE
    MICHAEL CHARNESS; Fiscal Year: 1999
    ..This knowledge will improve our understanding of the pathogenesis of fetal alcohol syndrome and cognitive disorders in alcoholics, paving the way for more effective prevention and treatment of these disorders. ..
  8. MACROLIDE TOTAL SYNTHESIS
    AMOS SMITH; Fiscal Year: 1990
    ..Once such features are identified, the design of new and possible more effective agents should be feasible...
  9. Cytoplasmic Reactivation of p53 Family Members
    Charles Walker; Fiscal Year: 2004
    ..This phenomenon in clams reflects that seen in a subset of human cancers and is inducible in mouse NIH 3T3 cells. In all of these cases, wild type p53 does not function because it is retained in the cytoplasm...
  10. METABLASTIN AND THE CONTROL OF CELL DIVISION
    ULRICH SCHUBART; Fiscal Year: 1999
    ..The proposed studies promise to shed light on a novel mechanism in the control cell replication, perturbations of which may play a role in oncogenesis. ..
  11. ODC AS A THERAPEUTIC TARGET--GENE FUNCTION ANALYSIS
    Gordon Luk; Fiscal Year: 1992
    ..Normal human fibroblasts, NIH 3T3 cells and human colon and lung carcinoma cell lines will be transfected with the human ODC gene...
  12. Post-transcriptional Regulation of Serotonin Receptors
    RONALD EMESON; Fiscal Year: 2006
    ..It is anticipated that these studies will provide new insights concerning the regulation of cellular processes involved in the transduction of serotonergic signals and the role(s) of multiple serotonin receptors in the nervous system. ..
  13. MOLECULAR AND FUNCTIONAL ANALYSIS OF HUMAN FGF 1
    Ing Ming Chiu; Fiscal Year: 2000
    ..B will be generated through targeted gene replacement. Information obtained through this study will have important implications in understanding the proliferation of glial cells and progression of tumorigenesis. ..
  14. GROWTH FACTOR SIGNALING AND TRAFFIC IN ANGIOGENESIS
    Igor Prudovsky; Fiscal Year: 2005
    ..membrane assay in vivo, completely represses the expression of type I collagen synthesis in vitro and NIH 3T3 cells stably transfected with the soluble form of Jagged1 exhibit type I collagen-dependent chord-like structures ..
  15. ROLE OF PHOSPHOLIPASE C IN TRANSFORMATION
    Albert Wong; Fiscal Year: 1993
    ..It is anticipated that these studies will add to our knowledge of the basic mechanisms underlying transformation as well as providing potentially useful clinical information...
  16. Polyamine Suppression By Antizyme inducing Analogs
    John Mitchell; Fiscal Year: 2002
    ..ability of selected analogs to reverse a transformed cell phenotype will be assessed using H-ras-transfected NIH 3T3 cells. Antizyme induction studies will use rat liver (HTC) and hamster (CHO) cells, and will involve detection of ..
  17. Tool to Define the Antiproliferative Effects of Agmatine
    Joseph Satriano; Fiscal Year: 2006
    ..These pathways will have particular application to diabetes and IRI in kidney, models we plan to pursue. [unreadable] [unreadable]..
  18. MOLECULAR BIOLOGY OF B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA
    Elizabeth Newcomb; Fiscal Year: 1993
    ..of DNA-mediated-gene-transfer was used to introduce high molecular weight DNA from B-CLL cells into mouse NIH 3T3 cells were inoculated into nude mice...
  19. Host Cell Determinants and Retroviral Infection
    Ghalib Alkhatib; Fiscal Year: 2003
    ..The experimental methods will utilize procedures and reagents previously utilized by the investigator during his published description of the HIV coreceptors. ..
  20. TUMOR ANTIGENS EXPRESSED BY ONCOGENE-TRANSFORMED CELLS
    Jack Roth; Fiscal Year: 1991
    ..The use of NIH 3T3 cells transfected with high molecular weight DNA from human tumors as an immunogen has resulted in the production of ..
  21. C-ABL ONCOGENE IN RADIATION INDUCED THYROID CARCINOMA
    SAYEEDA ZAIN; Fiscal Year: 1990
    ..These investigations should provide insight into the role of c-abl in this model of radiation carcinogenesis and may lead to a better understasnding of the normal function of the c-abl oncogene...
  22. STABLE ISOTOPE LABELED PROTEINS AND DRUG DISPOSITION
    Fred Abramson; Fiscal Year: 2002
    ..experiments, the goals are to generate an unambiguous profile of the intracellular metabolites produced in NIH 3T3 cells, evaluate which of these peptide metabolites contribute to the activity of FGF-1, and identify the active ..
  23. PITUITARY TUMOR CELL CULTURES
    Shlomo Melmed; Fiscal Year: 1991
    ..These studies will provide further insight into the pathogenesis of the cell-specific anterior pituitary adenomas...
  24. ALTERED EGF RECEPTORS IN HUMAN GLIAL TUMORS
    Albert Wong; Fiscal Year: 2001
    ..The structure of alternative transcripts from the Gab1 gene in tumors will be determined and their effects on transformation and signaling will be evaluated. ..
  25. DERIVATION OF REVERTANTS OF RAS-TRANSFORMED HUMAN CELLS
    LEONARD BENADE; Fiscal Year: 1992
    ..revertants using amphotropic murine leukemia virus (MuLV) as a helper and assaying focus-forming activity on NIH 3T3 cells. The presence of the ras gene product p21 will be confirmed by Western (protein) blotting...
  26. CAVEOLIN-3 AND MUSCULAR DYSTROPHY
    Michael Lisanti; Fiscal Year: 2004
    ..We will examine the phenotypic behavior of LGMD-1C mutations of caveolin-3 after heterologous expression in NIH 3T3 cells, as compared with wild-type caveolin-3; 2) To develop transgenic mouse models that over wild-type caveolin-3 ..
  27. ONCOGENES IN CARCINOGEN-INITIATED HAMSTER TUMOR CELLS
    Vicente Notario; Fiscal Year: 1993
    ..4) The presence of active oncogenes in other carcinogen-initiated hamster cell lines will be studied following similar experimental schemes to the ones used to find the new hamster oncogene...
  28. Jak2 involvement in Bcr-Abl oncogenic transformation
    Ralph Arlinghaus; Fiscal Year: 2005
    ..induction through Jak2/Bcr-Abl; 3) Investigate the role of the IL-3/GM-CSF receptors in the conversion of NIH 3T3 cells to a permissive phenotype for oncogenic transformation by BCR-ABL; NIH 3T3 cells are resistant to foci-..
  29. PPK: Radiation Response and Cell Proliferation
    Peter Stambrook; Fiscal Year: 2006
    ..Curiously, unlike its close relative Plk1, which transforms NIH 3T3 cells following transfection, Plk3 causes mammalian cells to undergo apoptosis following transfection and arrests ..
  30. SIGNALING VIA THE MULTIFUNCTIONAL TRIO PROTEIN
    Haruo Saito; Fiscal Year: 2001
    ..Expression of the Trio rac1-specific GEF domain also causes anchorage independent growth of NIH 3T3 cells indicating that Trio is a potential proto-oncogene...
  31. STRUCTURES OF NEURECTODERM TUMOR GLYCOPROTEINS
    MARY GLICK; Fiscal Year: 1992
    ..In part B we will address the multiantennary oligosaccharides. NIH 3T3 cells which have been transformed by transfection of human tumor DNA will be used...
  32. STRUCTURE/FUNCTION STUDY OF NA+/K+ ATPASE
    Lois Lane; Fiscal Year: 1999
    ..K-ATPase. These studies are currently ongoing in the applicant's laboratory. Good progress on specific aims 1 and 2 has already been achieved and initial studies on aim 3 are underway. ..
  33. MECHANISM OF GROWTH REGULATION BY EGR-1
    Dan Mercola; Fiscal Year: 2002
    ..Loss or defects in EGR-1 is a potential means of disrupting growth control by TGF- beta 1 and this is the tested here (AIM 2). ..
  34. Indomethacin and p38 Regulating AP-1 In Heat Shock
    Jacob Locke; Fiscal Year: 2004
    ..AP-1 DNA-binding activity via a mechanism involving the redox sensitive signaling factor Ref-1 in HeLa and NIH 3T3 cells; (2) OC-14 peroxide resistant cells overexpress c-Fos, c-Jun, and exhibit elevated AP-1 DNA activity when ..
  35. MECHANSISM OF CELL GROWTH INHIBITION BY RNASE-L
    Bret Hassel; Fiscal Year: 2001
    ....
  36. Molecular Analysis of Murine Leukemia Virus Entry
    Lorraine Albritton; Fiscal Year: 2007
    ..In addition, XC cells are more sensitive to cholesterol depletion than NIH 3T3 cells, together suggesting that the differences in fusion kinetics may result from lower receptor levels and ..
  37. ANTIBODIES AGAINST PHOSPHORYLATED 5HT2C RECEPTORS
    JON BACKSTROM; Fiscal Year: 2000
    ..The long-term goal of this research is to address the role of 5-HT2C receptors in epilepsy and hallucinogenic drug activity. ..
  38. Novel Inhibitors of Sphingosine Kinase
    KEVIN FRENCH; Fiscal Year: 2004
    ....
  39. INHIBITION OF HUMAN ONCOGENE EXPRESSION BY INTERFERON
    Robert Friedman; Fiscal Year: 2003
    ..In NIH 3T3 cells transformed by multiple copies of LTR-C-H-ras (RS485), the transcription of LO is markedly decreased...
  40. AKT2 ONCOGENE AND HUMAN ONCOGENESIS
    Jin Cheng; Fiscal Year: 2001
    ..I recently demonstrated that overexpression of AKT2 can transform NIH 3T3 cells, whereas AKT1 has not been shown to be oncogenic...
  41. The Fnk Kinase and Vascular Cell Growth Control
    JEFFREY WINKLES; Fiscal Year: 2004
    ..We have used a differential display approach to identify FGF-inducible genes in NIH 3T3 cells and vascular cells...
  42. Nutrient Inhibition of Ras-Rho Crosstalk
    Robert Hardy; Fiscal Year: 2007
    ..One recent study indicated that in NIH 3T3 cells oncogenic Ras activates RhoA by inhibiting Ras induced p190Rho-GAP sequestration in a detergent resistant ..
  43. HUMAN DBL ONCOGENE PRODUCT
    Shiv Srivastava; Fiscal Year: 1992
    ..Furthermore, the distribution and levels of db1 protein will be evaluated in human neoplasms...
  44. INTERACTION OF SRC AND OTHER PROTEINS IN TRANSFORMATION
    David Shalloway; Fiscal Year: 1992
    ..tyrosine kinase, and the nuclear oncogenes adeno E1A, polyoma large T (plt) and myc can induce transformation NIH 3T3 cells and that gap junctional communication is downregulated by overexpression of pp60c-src and activated mutants...
  45. CLONING OF A PHOSPHATIDYLINOSITOL KINASE
    Linda Pike; Fiscal Year: 1993
    ..The PI kinase cDNA will be cloned into an appropriate expression vector and expressed in NIH 3T3 cells and in BC3H1 cells, a mouse fibroblast that differentiates into a myocyte-like cell...
  46. EFFECTS OF ONCOGENES ON ION TRANSPORT
    Martin Lubin; Fiscal Year: 1990
    ....
  47. CYTOPLASMIC ROLE IN CARCINOGEN-INDUCED TUMORIGENICITY
    Jerry Shay; Fiscal Year: 1992
    ..By examining which of the mitochondrial DNA eleven Mspl sites have been eliminated, we will be able to pinpoint the mutated gene(s)...
  48. Antimicrobial Gene Therapy
    George Huang; Fiscal Year: 2003
    ....
  49. JUN KINASE AND HUMAN TUMORS
    Dan Mercola; Fiscal Year: 2001
    ....
  50. MURINE GAMMAHERPESVIRUS 68 GENE EXPRESSION AND FUNCTION
    SAMUEL DUBOISE; Fiscal Year: 2001
    ....
  51. AML1/ETO FUSION PROTEIN AND LEUKEMOGENESIS
    Richard Frank; Fiscal Year: 2000
    ..influence normal hematopoietic cell growth and development, 2) Define the ability of AML1/ETO to transform NIH 3T3 cells, and determine the effect of introducing the AML1/ETO fusion gene, by retroviral gene transfer techniques, on ..
  52. INTRACELLULAR TARGETS IN RETRO RETINOID SIGNALING
    Jochen Buck; Fiscal Year: 2000
    ..In general, AR has been shown to block activation and cause cell death in retinol-dependent cell lines, i.e., NIH 3T3 cells, and both effects can be prevented by 14-HRR in a dose-dependent manner...
  53. SAM68 AND HIV REPLICATION
    THIPPARTHI REDDY; Fiscal Year: 2002
    ..This Aim will also generate an in vitro model for the enhancement of HIV replication in NIH 3T3 cells by Sam68; 3) to assess the long-term protection of primary cells expressing transdominant mutants of Sam68 ..
  54. NOVEL, INDUCIBLE NUCLEAR KINASE LINKED TO LEUKEMIA
    GERALD DENIS; Fiscal Year: 2001
    ..The central hypothesis to be tested is that RING3 kinase is part of a signal transduction pathway that includes ALL-1 and is deregulated in leukemia. ..
  55. The function of I(3)mbt in hematopoietic cancers
    Stephen Nimer; Fiscal Year: 2007
    ..l(3)mbt we will express wild type and mutant forms of l(3)mbt in hematopoietic cells, and in Ras transformed NIH 3T3 cells. We will also assess how the lack of l(3)mbt affects hematopoiesis by generating l(3)mbt conditional knock-out ..
  56. REGULATION OF PROTEIN TURNOVER IN SEPSIS
    Scot R Kimball; Fiscal Year: 2010
    ....
  57. Oncogenic properties of interferon regulatory factor 7
    Luwen Zhang; Fiscal Year: 2009
    ..This study should provide an insight of how IRF-7 participates in EBV transformation, and expand our knowledge about IRFs as well as the pathogenesis of EBV-associated CNS lymphoma. ..
  58. A Nucleosomal Biosensor for Identification and Isolation of Nuclear Hormone Recep
    Richard Steinman; Fiscal Year: 2008
    ..This biosensor should be more rapid and versatile than cell-based reporter gene assays and more informative than assays that measure only ligand/receptor binding. [unreadable] [unreadable] [unreadable]..
  59. Acute Ethanol-Induced Innate Immune Response in Liver
    Richard Rippe; Fiscal Year: 2007
    ..The aims below will use gene delivery techniques and knockout mouse technology to address these critical questions related to roles of LPS, PI3 kinase activation, and CD14 expression in both acute ethanol and chronic ethanol toxicity. ..
  60. Mechanisms of Response to GnRH Receptor Signaling
    MARK LAWSON; Fiscal Year: 2005
    ..Isolation of actively translated and total mRNA from treated and control cells will allow identification and quantification of global changes in gene expression. ..
  61. E1A-INDUCED CELLULAR SENSITIZATION TO APOPTOSIS
    James Cook; Fiscal Year: 2002
    ..abstract_text> ..
  62. FIBRONECTIN AND RECEPTORS IN LUNGS VASCULOGENESIS
    Jesse Roman; Fiscal Year: 2003
    ..abstract_text> ..
  63. Inhibition of Androgen Receptor to treat Prostate Cancer
    Gayathri Devi; Fiscal Year: 2003
    ..Based on these data, future pharmacokinetic and toxicology animal studies will allow proceeding to human phase I testing in a timely and efficient manner. ..
  64. Skin Manifestations of Tuberous Sclerosis
    Jack Arbiser; Fiscal Year: 2009
    ..Specific Aim 3. To determine whether dysregulation of p16ink4a and PTCH (patched) acts as a modifier of theTS phenotype in transgenic mice expressing dominant negative tuberin. ..