3t3 cells


Summary: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

Top Publications

  1. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
  2. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
  3. pmc Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
    D Riveline
    Laboratory of Physical Spectrometry (CNRS, UMR 5588, Joseph Fourier University, French National Center for Scientific Research, BP87, , France
    J Cell Biol 153:1175-86. 2001
  4. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
  5. pmc The relationship between force and focal complex development
    Catherine G Galbraith
    Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 159:695-705. 2002
  6. ncbi Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor
    P Tontonoz
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115
    Cell 79:1147-56. 1994
  7. pmc Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
    T Matsui
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    EMBO J 15:2208-16. 1996
  8. ncbi mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer
    P Tontonoz
    Dana Farber Cancer Institute, Boston, Massachusetts
    Genes Dev 8:1224-34. 1994
  9. pmc Measurement of mechanical tractions exerted by cells in three-dimensional matrices
    Wesley R Legant
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Methods 7:969-71. 2010
  10. ncbi Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes
    Deepanwita Prusty
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:46226-32. 2002

Detail Information

Publications307 found, 100 shown here

  1. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..rapidly stimulated stress fiber and focal adhesion formation when microinjected into serum-starved Swiss 3T3 cells. Readdition of serum produced a similar response, detectable within 2 min...
  2. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC...
  3. pmc Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
    D Riveline
    Laboratory of Physical Spectrometry (CNRS, UMR 5588, Joseph Fourier University, French National Center for Scientific Research, BP87, , France
    J Cell Biol 153:1175-86. 2001
    ..Our experiments show that integrin-containing focal complexes behave as individual mechanosensors exhibiting directional assembly in response to local force...
  4. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles...
  5. pmc The relationship between force and focal complex development
    Catherine G Galbraith
    Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 159:695-705. 2002
  6. ncbi Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor
    P Tontonoz
    Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115
    Cell 79:1147-56. 1994
  7. pmc Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
    T Matsui
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    EMBO J 15:2208-16. 1996
    ..Thus it is likely that Rho-kinase is a putative target serine/threonine kinase for Rho and serves as a mediator of the Rho-dependent signaling pathway...
  8. ncbi mPPAR gamma 2: tissue-specific regulator of an adipocyte enhancer
    P Tontonoz
    Dana Farber Cancer Institute, Boston, Massachusetts
    Genes Dev 8:1224-34. 1994
  9. pmc Measurement of mechanical tractions exerted by cells in three-dimensional matrices
    Wesley R Legant
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Methods 7:969-71. 2010
    ..Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix...
  10. ncbi Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma ) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes
    Deepanwita Prusty
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:46226-32. 2002
    ..Our results suggest that activation of the MEK/ERK signaling pathway during the initial 12 h of adipogenesis enhances the activity of factors that regulate both C/EBPalpha and PPARgamma expression...
  11. pmc Stresses at the cell-to-substrate interface during locomotion of fibroblasts
    M Dembo
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215 2407, USA
    Biophys J 76:2307-16. 1999
    ..These observations demonstrate that the lamellipodium of the fibroblast is able to generate intense traction stress. The cell body and posterior seem to be mechanically passive structures pulled forward entirely by this action...
  12. ncbi The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF
    C S Hill
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England
    Cell 81:1159-70. 1995
    ..Functional Rho is required for regulated activity of the c-fos promoter. These results establish SRF as a nuclear target of a novel Rho-mediated signaling pathway...
  13. ncbi A role for Rac in Tiam1-induced membrane ruffling and invasion
    F Michiels
    The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam
    Nature 375:338-40. 1995
    ..Moreover, T-lymphoma cells expressing V12Rac1 become invasive, indicating that the Tiam1-Rac signalling pathway could be operating in the invasion and metastasis of tumour cells...
  14. ncbi The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin
    Mayumi Inoue
    Life Sciences Institute, Departments of Internal Medicine and Physiology, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Nature 422:629-33. 2003
    ..So, the exocyst might have a crucial role in the targeting of the Glut4 vesicle to the plasma membrane, perhaps directing the vesicle to the precise site of fusion...
  15. ncbi CAP defines a second signalling pathway required for insulin-stimulated glucose transport
    C A Baumann
    Department of Physiology, University of Michigan School of Medicine, Ann Arbor, Michigan 48109, USA
    Nature 407:202-7. 2000
    ..Thus, localization of the Cbl-CAP complex to lipid rafts generates a pathway that is crucial in the regulation of glucose uptake...
  16. ncbi 15-Deoxy-delta 12, 14-prostaglandin J2 is a ligand for the adipocyte determination factor PPAR gamma
    B M Forman
    Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Cell 83:803-12. 1995
    ..These findings suggest a pivotal role for PPAR gamma and its endogenous ligand in adipocyte development and glucose homeostasis and as a target for intervention in metabolic disorders...
  17. ncbi Regulation of protein kinase C zeta by PI 3-kinase and PDK-1
    M M Chou
    University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, Philadelphia 19104, USA
    Curr Biol 8:1069-77. 1998
    ..We therefore investigated whether PDK-1 is the kinase that activates PKC zeta...
  18. ncbi Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation
    Hiroyuki Sano
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA
    J Biol Chem 278:14599-602. 2003
    ..These findings strongly indicate that insulin-stimulated phosphorylation of AS160 is required for GLUT4 translocation and that this phosphorylation signals translocation through inactivation of the Rab GAP function...
  19. ncbi Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
    M A Sells
    Fox Chase Cancer Centre, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Curr Biol 7:202-10. 1997
    ..This kinase is therefore a candidate effector for the changes in cell shape induced by growth factors...
  20. pmc Distinct roles of ROCK (Rho-kinase) and MLCK in spatial regulation of MLC phosphorylation for assembly of stress fibers and focal adhesions in 3T3 fibroblasts
    G Totsukawa
    Department of Molecular Biology and Biochemistry, Rutgers University, Nelson Lab, Busch Campus, Piscataway, New Jersey 08855, USA
    J Cell Biol 150:797-806. 2000
    ..At the cell periphery, on the other hand, MLCK but not ROCK appears to be the kinase responsible for phosphorylating MLC. These results suggest that ROCK and MLCK play distinct roles in spatial regulation of MLC phosphorylation...
  21. ncbi An essential role for NF-kappaB in preventing TNF-alpha-induced cell death
    A A Beg
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 274:782-4. 1996
    ..These results have implications for the treatment of inflammatory and proliferative diseases...
  22. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
    ..Immunoblotting of serum-starved Swiss 3T3 cells with this mAb revealed that after LPA stimulation ERM proteins were rapidly phosphorylated at T567 (ezrin), T564 ..
  23. pmc Substrate topography induces a crossover from 2D to 3D behavior in fibroblast migration
    Marion Ghibaudo
    Laboratoire Matiere et Systemes Complexes MSC, Université Paris Diderot and Centre National de la Recherche Scientifique, Unite Mixte de Recherche 7057, Paris, France
    Biophys J 97:357-68. 2009
    ..Finally, the analysis of cell movements at the frontier between flat and micropillar substrates shows that cell transmigration through the micropillar substrates depends on the spacing between the pillars...
  24. ncbi Development of an efficient "substrate-trapping" mutant of Src homology phosphotyrosine phosphatase 2 and identification of the epidermal growth factor receptor, Gab1, and three other proteins as target substrates
    Yehenew M Agazie
    Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, 11794 5222, USA
    J Biol Chem 278:13952-8. 2003
    ..Therefore, the DM protein would serve as an important tool in future SHP2 studies, including identification of p190, p150, and p90...
  25. ncbi Effect of low- and high-intensity pulsed ultrasound on collagen post-translational modifications in MC3T3-E1 osteoblasts
    M Saito
    Department of Orthopaedic Surgery, Jikei University School of Medicine, 3 25 8, Nishi Skinbaski, Minato ku, Tokyo, 105 8461, Japan
    Calcif Tissue Int 75:384-95. 2004
  26. ncbi Sclerostin binds to LRP5/6 and antagonizes canonical Wnt signaling
    Xiaofeng Li
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, 06030, USA
    J Biol Chem 280:19883-7. 2005
  27. ncbi Adipocyte-derived serum amyloid A3 and hyaluronan play a role in monocyte recruitment and adhesion
    Chang Yeop Han
    Department of Medicine, University of Washington, Seattle, Washington 98195 6426, USA
    Diabetes 56:2260-73. 2007
    ..We hypothesize that adipocyte hypertrophy leads to increased production of SAA and hyaluronan, which act in concert to recruit and retain monocytes, thereby leading to local inflammation in adipose tissue...
  28. pmc Mitotic clonal expansion: a synchronous process required for adipogenesis
    Qi Qun Tang
    Department of Biological Chemistry, Division of Endocrinology, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 100:44-9. 2003
    ..These results show that MCE is a prerequisite for differentiation of 3T3-L1 preadipocytes into adipocytes...
  29. pmc A novel oncogene, ost, encodes a guanine nucleotide exchange factor that potentially links Rho and Rac signaling pathways
    Y Horii
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 13:4776-86. 1994
    ..Of the tissues examined, expression of ost was the highest in brain and could be localized to neurons and alpha-tanycytes, suggesting that Ost may participate in axonal transport in these specialized cells...
  30. ncbi RET/PTC1 oncogene signaling in PC Cl 3 thyroid cells requires the small GTP-binding protein Rho
    M V Barone
    Centro di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, , Via S. Pansini 5, Naples, Italy
    Oncogene 20:6973-82. 2001
    ..We conclude that Rho is implicated in the actin reorganization and cell survival mediated by the chimeric RET/PTC1 oncogene in thyroid epithelial cells, both phenotypes being cell type- and oncogene type-specific...
  31. ncbi Integrin and cytoskeletal regulation of growth factor signaling to the MAP kinase pathway
    A E Aplin
    Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Sci 112:695-706. 1999
  32. pmc Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation
    M M Kessels
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720 3202, USA
    Mol Biol Cell 11:393-412. 2000
    ..mAbp1 is a newly identified cytoskeletal protein in mice and may serve as a signal-responsive link between the dynamic cortical actin cytoskeleton and regions of membrane dynamics...
  33. pmc TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle
    Ding An
    Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 59:1358-65. 2010
    ..Genetic linkage analyses show a TBC1D1 R125W missense variant confers risk for severe obesity in humans. The objective of this study was to determine whether TBC1D1 regulates glucose transport in skeletal muscle...
  34. ncbi Activation of p38 mitogen-activated protein kinase and c-Jun-NH2-terminal kinase by BMP-2 and their implication in the stimulation of osteoblastic cell differentiation
    J Guicheux
    Division of Bone Diseases, Department of Geriatrics, University Hospital of Geneva, Geneva, Switzerland
    J Bone Miner Res 18:2060-8. 2003
    ..We report that BMP-2 can activate JNK and p38 in osteoblastic cells and provide evidences that these MAP kinases have distinct roles in regulating alkaline phosphatase and osteocalcin expression...
  35. ncbi Acute downregulation of connexin43 at wound sites leads to a reduced inflammatory response, enhanced keratinocyte proliferation and wound fibroblast migration
    Ryoichi Mori
    Department of Physiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 119:5193-203. 2006
  36. pmc Cells lying on a bed of microneedles: an approach to isolate mechanical force
    John L Tan
    Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 100:1484-9. 2003
  37. ncbi Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2
    Nanao Horike
    Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Osaka University, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biol Chem 278:18440-7. 2003
  38. ncbi Glucocorticoids inhibit developmental stage-specific osteoblast cell cycle. Dissociation of cyclin A-cyclin-dependent kinase 2 from E2F4-p130 complexes
    E Smith
    Department of Orthopaedic Surgery, Institute for Genetic Medicine, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Biol Chem 275:19992-20001. 2000
    ..Thus, glucocorticoids induce premature attenuation of the osteoblast cell cycle, possibly contributing to the osteoporosis induced by these drugs in vivo...
  39. ncbi The 230 kDa mature form of KDR/Flk-1 (VEGF receptor-2) activates the PLC-gamma pathway and partially induces mitotic signals in NIH3T3 fibroblasts
    T Takahashi
    Department of Genetics, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    Oncogene 14:2079-89. 1997
    ..These results suggest that the KDR/Flk-1 utilizes cell type-specific signal transduction pathway(s) for MAP kinase activation and the mitotic response in endothelial cells...
  40. ncbi The critical role of Shc in insulin-like growth factor-I-mediated mitogenesis and differentiation in 3T3-L1 preadipocytes
    C M Boney
    Department of Pediatrics Rhode Island Hospital, Providence 02903, USA
    Mol Endocrinol 14:805-13. 2000
    ..Our results suggest that loss of the ability of IGF-I to activate Shc signaling to MAPK may be an early component of adipogenesis in 3T3-L1 cells...
  41. pmc Partial lipodystrophy and insulin resistant diabetes in a patient with a homozygous nonsense mutation in CIDEC
    Oscar Rubio-Cabezas
    Department of Endocrinology, Hospital Infantil Universitario Nino Jesus, Madrid, Spain
    EMBO Mol Med 1:280-7. 2009
    ..In addition to describing a novel autosomal recessive form of familial partial lipodystrophy, these observations also suggest that CIDEC is required for unilocular lipid droplet formation and optimal energy storage in human fat...
  42. ncbi Characterization of the nuclear import and export functions of Ikappa B(epsilon)
    Sang Hyun Lee
    Biochemistry Department University of Missouri, Columbia, Missouri 65212, USA
    J Biol Chem 277:23358-66. 2002
  43. pmc Secretion of the adipocyte-specific secretory protein adiponectin critically depends on thiol-mediated protein retention
    Zhao V Wang
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 27:3716-31. 2007
    ..One mechanism for increasing circulating levels of specific adiponectin complexes by peroxisome proliferator-activated receptor gamma agonists may be selective upregulation of rate-limiting chaperones...
  44. ncbi Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin
    Hironori Waki
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113 8655, Japan
    J Biol Chem 278:40352-63. 2003
    ..In conclusion, not only total concentrations, but also multimer distribution should always be considered in the interpretation of plasma adiponectin levels in health as well as various disease states...
  45. ncbi Cell surface localization of tissue transglutaminase is dependent on a fibronectin-binding site in its N-terminal beta-sandwich domain
    C A Gaudry
    Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom
    J Biol Chem 274:30707-14. 1999
    ..These results demonstrate that tTG binds to the pericellular fibronectin coat of cells via its N-terminal beta-sandwich domain and that this interaction is crucial for cell surface association of tTG...
  46. ncbi Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts
    J You
    Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, USA
    J Biol Chem 276:13365-71. 2001
    ..In addition, experiments using a variety of pharmacological agents suggest that oscillatory flow induces Ca(2+)i mobilization via the L-type voltage-operated calcium channel and the inositol 1,4,5-trisphosphate pathway...
  47. ncbi The regulation of fatty acid synthase by STAT5A
    Jessica C Hogan
    Louisiana State University, Department of Biological Sciences, 202 Life Sciences Bldg, Baton Rouge, LA 70803, USA
    Diabetes 54:1968-75. 2005
    ..Furthermore, our results indicate that STAT5A has an antilipogenic function in adipocytes and may contribute to the regulation of energy balance...
  48. pmc The ornithine decarboxylase gene is a transcriptional target of c-Myc
    C Bello-Fernandez
    Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN 38105
    Proc Natl Acad Sci U S A 90:7804-8. 1993
    ..This suggests that Myc may interact with transcription factors other than Max to transactivate the ODC gene...
  49. ncbi The effect of biomimetic apatite structure on osteoblast viability, proliferation, and gene expression
    Yu Fen Chou
    Department of Bioengineering, University of California, Los Angeles, CA 90095, USA
    Biomaterials 26:285-95. 2005
    ..The results of this study suggest differential cell responses to subtle changes in apatite microenvironment...
  50. ncbi Timing of cyclin D1 expression within G1 phase is controlled by Rho
    C F Welsh
    Department of Medicine and Sylvester Cancer Center, University of Miami School of Medicine, 1475 NW 12th Ave, Miami, Florida 33136, USA
    Nat Cell Biol 3:950-7. 2001
    ..Our results show that Rho is crucial for maintaining the correct timing of cyclin D1 expression in G1 phase and describe a new role for cytoskeletal integrity in the regulation of cell cycle progression...
  51. ncbi Insulin-stimulated GLUT4 translocation in adipocytes is dependent upon cortical actin remodeling
    M Kanzaki
    Department of Physiology and Biophysics, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 276:42436-44. 2001
    ..In addition, cortical F-actin does not function in a static manner (e.g. barrier or scaffold), but insulin-stimulated dynamic cortical actin remodeling is necessary for the GLUT4 translocation process...
  52. ncbi Oligomerization state-dependent activation of NF-kappa B signaling pathway by adipocyte complement-related protein of 30 kDa (Acrp30)
    Tsu Shuen Tsao
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    J Biol Chem 277:29359-62. 2002
    ..Our data indicate that oligomerization of Acrp30 is important for at least some of its biological activities, and changes in the relative abundance of each oligomeric isoform in plasma may regulate Acrp30 activity...
  53. pmc Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion
    R L DeBiasio
    Division of Molecular Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Mol Biol Cell 7:1259-82. 1996
    ..We have investigated the structural and chemical dynamics of myosin II in living Swiss 3T3 cells from prometaphase through the separation and migration of daughter cells...
  54. pmc Distinct dynamics of endocytic clathrin-coated pits and coated plaques
    Saveez Saffarian
    Department of Cell Biology, Harvard Medical School, Children s Hospital and Immune Disease Institute, Boston, Massachusetts, USA
    PLoS Biol 7:e1000191. 2009
  55. ncbi The tyrosine kinase c-Abl regulates p73 in apoptotic response to cisplatin-induced DNA damage
    J G Gong
    Department of Biology, University of California, San Diego, La Jolla 92093 0322, USA
    Nature 399:806-9. 1999
    ..Our results indicate that c-Abl and p73 are components of a mismatch-repair-dependent apoptosis pathway which contributes to cisplatin-induced cytotoxicity...
  56. ncbi Apoptosis and survival of osteoblast-like cells are regulated by surface attachment
    Vavara Grigoriou
    Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 280:1733-9. 2005
    ..These results indicated that the binding of cells to RGDS abrogated apoptosis via the mitochondrial pathway and that the suppression of apoptosis was dependent on the activity of phosphatidylinositol 3-kinase...
  57. ncbi Protein phosphatase 2A positively regulates Ras signaling by dephosphorylating KSR1 and Raf-1 on critical 14-3-3 binding sites
    Stephane Ory
    Regulation of Cell Growth Laboratory, NCI Frederick, Frederick, MD 21702, USA
    Curr Biol 13:1356-64. 2003
    ..Although recruitment of KSR1 from the cytosol to the plasma membrane is required for its scaffolding function, the precise mechanism(s) regulating the translocation of KSR1 have not been fully elucidated...
  58. pmc The small GTPase RalA targets filamin to induce filopodia
    Y Ohta
    Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4 1 1 Ogawahigashi, Kodaira, Tokyo 187, Japan
    Proc Natl Acad Sci U S A 96:2122-8. 1999
    ..only RalA binds filamin in a GTP-specific manner, and GTP-RalA elicits actin-rich filopods on surfaces of Swiss 3T3 cells and recruits filamin into the filopodial cytoskeleton...
  59. ncbi The emergence of ECM mechanics and cytoskeletal tension as important regulators of cell function
    Shelly R Peyton
    Department of Chemical Engineering and Materials Science, The Henry Samueli School of Engineering, University of California, Irvine, CA 92697 2715, USA
    Cell Biochem Biophys 47:300-20. 2007
  60. ncbi Myc but not Fos rescue of PDGF signalling block caused by kinase-inactive Src
    M V Barone
    Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    Nature 378:509-12. 1995
    ..In contrast, Fos and Jun, but not Myc, rescued the block induced by dominant negative Ras. Our data suggest that Src kinases control the transcriptional activation of Myc...
  61. ncbi Phosphotyrosyl peptides block Stat3-mediated DNA binding activity, gene regulation, and cell transformation
    J Turkson
    Molecular Oncology and Drug Discovery Programs, H Lee Moffitt Cancer Center and Research Institute, University of South Florida College of Medicine, Tampa, Florida 33612, USA
    J Biol Chem 276:45443-55. 2001
    ..Altogether, we have identified a minimal peptide that inhibits Stat3 signaling and provides the conceptual basis for use of this peptide as a lead for novel peptidomimetic drug design...
  62. ncbi Modulation of adipose tissue expression of murine matrix metalloproteinases and their tissue inhibitors with obesity
    Erik Maquoi
    Center for Molecular and Vascular Biology, University of Leuven, Belgium
    Diabetes 51:1093-101. 2002
    ..Moreover, this in vitro adipogenesis was reduced in the presence of a synthetic MMP inhibitor. Thus, the adipose tissue expresses a large array of MMPs and TIMPs, which modulate adipocyte differentiation...
  63. pmc Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence
    Jochen Guck
    Institute for Soft Matter Physics, Department of Physics and Geosciences, University of Leipzig, 04103 Leipzig, Germany
    Biophys J 88:3689-98. 2005
    ..This suggests using optical deformability as an inherent cell marker for basic cell biological investigation and diagnosis of disease...
  64. ncbi Endoplasmic reticulum stress induced by aqueous extracts of cigarette smoke in 3T3 cells activates the unfolded-protein-response-dependent PERK pathway of cell survival
    Arnd Hengstermann
    Philip Morris Research Laboratories GmbH, Fuggerstr 3, D 51149 Cologne, Germany
    Free Radic Biol Med 44:1097-107. 2008
    ..of aqueous extracts of CS induce a distinct ER stress response in immortalized nontransformed Swiss 3T3 cells, primarily by activating the PERK pathway of global protein synthesis inhibition...
  65. ncbi Control of apoptosis and mitotic spindle checkpoint by survivin
    F Li
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nature 396:580-4. 1998
    ..The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis...
  66. ncbi Notch-1 signalling requires ligand-induced proteolytic release of intracellular domain
    E H Schroeter
    Department of Molecular Biology and Pharmacology, Washington University, St Louis, Missouri 63110, USA
    Nature 393:382-6. 1998
    ..Very small amounts of NICD are active, explaining why it is hard to detect in the nucleus in vivo. We also show that it is ligand binding that induces release of NICD...
  67. ncbi Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts
    Alexander Gray
    Division of Signal Transduction Therapy, MSI WTB complex, University of Dundee, Dow Street, Scotland, Dundee DD1 5EH, UK
    Anal Biochem 313:234-45. 2003
    ..This system was used to monitor the accumulation of PtdIns(3,4)P(2) in Swiss 3T3 cells exposed to an oxidative stress...
  68. pmc Stat3 activation by Src induces specific gene regulation and is required for cell transformation
    J Turkson
    H Lee Moffitt Cancer Center and Research Institute, and Department of Biochemistry and Molecular Biology, University of South Florida College of Medicine, Tampa 33612, USA
    Mol Cell Biol 18:2545-52. 1998
    ..expression, luciferase reporter constructs based on synthetic and authentic promoters were transfected into NIH 3T3 cells. Activation of endogenous cellular Stat3 by the Src oncoprotein induced gene expression through a Stat3-specific ..
  69. ncbi Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts
    C M Waterman-Storer
    Department of Biology, University of North Carolina, Chapel Hill 27599, USA
    Nat Cell Biol 1:45-50. 1999
    ..We suggest that the growth phase of microtubule dynamic instability at leading-edge lamellipodia locally activates Rac1 to drive actin polymerization and lamellipodial protrusion required for cell migration...
  70. pmc Mammalian target of rapamycin complex 1 (mTORC1) plays a role in Pasteurella multocida toxin (PMT)-induced protein synthesis and proliferation in Swiss 3T3 cells
    Hammou Oubrahim
    Laboratory of Biochemistry, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 8012, USA
    J Biol Chem 288:2805-15. 2013
    ..Herein, we show that PMT induces protein synthesis, cell migration, and proliferation in serum-starved Swiss 3T3 cells. Concomitantly PMT induces phosphorylation of ribosomal S6 kinase (S6K1) and its substrate, ribosomal S6 protein ..
  71. pmc Myosin 1E interacts with synaptojanin-1 and dynamin and is involved in endocytosis
    Mira Krendel
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06511, USA
    FEBS Lett 581:644-50. 2007
    ..Expression of Myo1E tail, which acts in a dominant-negative manner, inhibits endocytosis of transferrin. Our findings suggest that myosin 1E may contribute to receptor-mediated endocytosis...
  72. ncbi Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation
    A D Kohn
    Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 271:31372-8. 1996
    ..The increased glucose influx in the 3T3-L1 adipocytes directed lipid but not glycogen synthesis. These results indicate that Akt can regulate glucose uptake and metabolism...
  73. pmc Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton
    X D Ren
    Department of Vascular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    EMBO J 18:578-85. 1999
    ..To address these questions, we established an assay for GTP-bound cellular Rho. Plating Swiss 3T3 cells on fibronectin-coated dishes elicited a transient inhibition of Rho, followed by a phase of Rho activation...
  74. pmc Phosphorylation of receptor protein-tyrosine phosphatase alpha on Tyr789, a binding site for the SH3-SH2-SH3 adaptor protein GRB-2 in vivo
    J den Hertog
    Salk Institute, Molecular Biology and Virology Laboratory, La Jolla, CA 92037
    EMBO J 13:3020-32. 1994
    ..In addition, RPTP alpha co-immunoprecipitated with GRB2 from NIH 3T3 cells, demonstrating that GRB2 bound to RPTP alpha in vivo...
  75. ncbi Genomic structure, cDNA sequence, and expression of gly96, a growth factor-inducible immediate-early gene encoding a short-lived glycosylated protein
    C H Charles
    Department of Genetics, University of Illinois College of Medicine, Chicago 60612
    Oncogene 8:797-801. 1993
    ..We have identified the Gly96 protein in Balb/c 3T3 cells using affinity-purified antibodies recognizing the Gly96 polypeptide...
  76. ncbi Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds
    Michael J Jaasma
    Department of Anatomy, Royal College of Surgeons in Ireland, 123 St Stephen s Green, Dublin 2, Ireland
    J Biotechnol 133:490-6. 2008
    ..This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications...
  77. ncbi Troglitazone antagonizes tumor necrosis factor-alpha-induced reprogramming of adipocyte gene expression by inhibiting the transcriptional regulatory functions of NF-kappaB
    Hong Ruan
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    J Biol Chem 278:28181-92. 2003
    ..Our data suggest that inhibition of NF-kappaB activity is a mechanism by which PPAR-gamma agonists improve insulin sensitivity in vivo and that adipocyte NF-kappaB is a potential therapeutic target for obesity-linked type 2 diabetes...
  78. pmc Eps8, a substrate for the epidermal growth factor receptor kinase, enhances EGF-dependent mitogenic signals
    F Fazioli
    Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892
    EMBO J 12:3799-808. 1993
    ..Adoptive expression of the eps8 cDNA in fibroblastic or hematopoietic target cells expressing the EGFR resulted in increased mitogenic response to EGF, implicating the eps8 gene product in the control of mitogenic signals...
  79. ncbi Design of a modular bioreactor to incorporate both perfusion flow and hydrostatic compression for tissue engineering applications
    David E Orr
    Department of Bioengineering, Clemson University, Clemson, SC 29634, USA
    Ann Biomed Eng 36:1228-41. 2008
    ..The bioreactor has established an experimental platform for ongoing investigation of the interactive effect of perfusion fluid flow and hydrostatic compression on multiple cell types...
  80. pmc Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)
    Thomas Dertinger
    Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, California, USA
    Opt Express 18:18875-85. 2010
    ..We show a two-fold increase of resolution (over the diffraction limit) of near-infrared quantum dot labeled tubulin-network of 3T3 fibroblasts...
  81. ncbi The mitogen-inducible Fn14 gene encodes a type I transmembrane protein that modulates fibroblast adhesion and migration
    R L Meighan-Mantha
    Department of Vascular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    J Biol Chem 274:33166-76. 1999
    ..have used a differential display approach to identify fibroblast growth factor-1-inducible genes in murine NIH 3T3 cells. Here, we report that the fibroblast growth factor-inducible-14 (Fn14) gene is a growth factor-regulated, ..
  82. ncbi Antibacterial and mechanical properties of bone cement impregnated with chitosan nanoparticles
    Zhilong Shi
    Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge, Singapore 119260, Singapore
    Biomaterials 27:2440-9. 2006
    ..These results indicate a new promising strategy for combating joint implant infection...
  83. pmc Substrate rigidity regulates the formation and maintenance of tissues
    Wei hui Guo
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Biophys J 90:2213-20. 2006
  84. pmc RNA splicing regulates the temporal order of TNF-induced gene expression
    Shengli Hao
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 110:11934-9. 2013
    ..We also examined the elongation times, concluding that pre-mRNA synthesis is coordinate but splicing differences directly regulate the timing of mRNA production. ..
  85. pmc Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription
    R Voit
    Division of Molecular Biology of the Cell II, German Cancer Research Center, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 98:13631-6. 2001
    ..The results suggest that phosphorylation of UBF represents a powerful means of modulating the assembly of the transcription initiation complex in a proliferation- and cell cycle-dependent fashion...
  86. pmc Isolation of cDNAs for perilipins A and B: sequence and expression of lipid droplet-associated proteins of adipocytes
    A S Greenberg
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 90:12035-9. 1993
    ..The molecular probes for perilipins A and B described here will permit detailed analyses of their functional role(s) in lipid metabolism...
  87. ncbi Rich, a rho GTPase-activating protein domain-containing protein involved in signaling by Cdc42 and Rac1
    N Richnau
    Ludwig Institute for Cancer Research, Biomedical Center, Box 595, S 751 24 Uppsala, Sweden
    J Biol Chem 276:35060-70. 2001
  88. ncbi Pulmonary inflammatory myofibroblastic tumor expressing a novel fusion, PPFIBP1-ALK: reappraisal of anti-ALK immunohistochemistry as a tool for novel ALK fusion identification
    Kengo Takeuchi
    Pathology Project for Molecular Targets, Division of Pathology, Thoracic Center, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan
    Clin Cancer Res 17:3341-8. 2011
    ..By using sensitive immunohistochemistry, therefore, we reassessed "ALK-negative" IMT cases defined with conventional immunohistochemistry (approximately 50% of all examined cases)...
  89. ncbi Selective bone cell adhesion on formulations containing carbon nanofibers
    Rachel L Price
    Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907 1296, USA
    Biomaterials 24:1877-87. 2003
    ..Moreover, greater weight percentages of high surface energy carbon nanofibers in the PCU/CNF composite increased osteoblast adhesion while at the same time decreased fibroblast adhesion...
  90. ncbi Three-dimensional growth of differentiating MC3T3-E1 pre-osteoblasts on porous titanium scaffolds
    Jean Philippe St-Pierre
    Department of Biomedical Engineering and Faculty of Dentistry, McGill University, Montreal, Que, Canada H3A 2B4
    Biomaterials 26:7319-28. 2005
    ..Thus, the microstructured titanium scaffolds direct the behaviour of pre-osteoblasts toward a mature state capable of mineralising the extracellular matrix...
  91. ncbi Excess iron inhibits osteoblast metabolism
    Kanako Yamasaki
    Department of Biomedical Engineering, Toin University of Yokohama, Yokohama 225 8502, Japan
    Toxicol Lett 191:211-5. 2009
    ..These results suggest that iron overload might give rise to osteoporosis by inhibiting osteoblast proliferation and differentiation...
  92. ncbi Bone morphogenetic protein-2 promotes the haptotactic migration of murine osteoblastic and osteosarcoma cells by enhancing incorporation of integrin beta1 into lipid rafts
    Tsukasa Sotobori
    Department of Biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1 3 3 Nakamichi, Higashinari ku, Osaka 537 8511, Japan
    Exp Cell Res 312:3927-38. 2006
    ..Collectively, these results provide insights into the mechanism by which BMP signaling enhances cell migration by modulating fibronectin-integrin beta1 signaling via cholesterol enriched membrane microdomains, lipid rafts...
  93. pmc Insulin stimulation of GLUT4 exocytosis, but not its inhibition of endocytosis, is dependent on RabGAP AS160
    Anja Zeigerer
    Department of Biochemistry, Weill Medical College of Cornell University, New York, NY 10021, USA
    Mol Biol Cell 15:4406-15. 2004
    ..These data support a model in which insulin signaling to the exocytosis machinery (AS160 dependent) is distinct from its signaling to the internalization machinery (AS160 independent)...
  94. ncbi Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment
    Juana Magdalena
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    J Cell Sci 116:743-56. 2003
    ..In a previous study we showed that Golgi reorientation in response to a scratch wound is actin-dependent in NIH 3T3 cells but not in astrocytes...
  95. pmc Quantitative analysis of lipid droplet fusion: inefficient steady state fusion but rapid stimulation by chemical fusogens
    Samantha Murphy
    The University of Queensland, Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, Brisbane, Australia
    PLoS ONE 5:e15030. 2010
    ..In addition, it highlights the extent of LD restructuring which occurs when homotypic LD fusion is triggered in a variety of cell types...
  96. pmc Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin
    Kengo Kitadokoro
    Research Center for Low Temperature and Materials Sciences, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Proc Natl Acad Sci U S A 104:5139-44. 2007
    ..Our results indicate that PMT is an enzyme toxin carrying the cysteine protease-like catalytic triad dependent on the redox state and functions on the cytoplasmic face of the plasma membrane of target cells...
  97. pmc APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes
    Jun Liu
    Department of Internal Medicine and Physiology, Life Science Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA
    Mol Cell Biol 22:3599-609. 2002
  98. ncbi TNF alpha-mediated inhibition and reversal of adipocyte differentiation is accompanied by suppressed expression of PPARgamma without effects on Pref-1 expression
    H Xing
    Affymax Research Institute, Santa Clara, California 95051, USA
    Endocrinology 138:2776-83. 1997
    ..Our results support the notion that dedifferentiated adipocytes and preadipocytes are not identical, though they share many similar morphological and gene expression patterns...
  99. ncbi JNK- and IkappaB-dependent pathways regulate MCP-1 but not adiponectin release from artificially hypertrophied 3T3-L1 adipocytes preloaded with palmitate in vitro
    Kazuto Takahashi
    Third Department of Internal Medicine, Kyorin University School of Medicine, Tokyo, Japan
    Am J Physiol Endocrinol Metab 294:E898-909. 2008
    ..In contrast, the parallel increase in adiponectin expression appears to be related to the PPARgamma ligand properties of palmitate...
  100. ncbi Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors
    Hisashi Arase
    Department of Microbiology and Immunology and the Cancer Research Institute, University of California San Francisco, San Francisco, CA 94143, USA
    Science 296:1323-6. 2002
    ..These activating and inhibitory receptors are highly homologous, suggesting the possibility that one evolved from the other in response to selective pressure imposed by the pathogen...
  101. ncbi Enhanced osteoblast adhesion on transglutaminase 2-crosslinked fibronectin
    J Forsprecher
    Division of Biomedical Sciences, Faculty of Dentistry, McGill University, 3640 University Street, Strathcona Building, Montreal, QC, Canada
    Amino Acids 36:747-53. 2009
    ..We hypothesize that TG2-mediated crosslinking enhances the cell-adhesive properties of FN by increasing the molecular rigidity of FN in the extracellular matrix...

Research Grants63

  1. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
    ..Ectopic expression of PRDM3 in non-adipogenic fibroblasts such as NIH-3T3 cells stimulates adipogenesis including induction of some BAT-related genes in a cAMP dependent manner like UCP1 and ..
    Michael R Green; Fiscal Year: 2013
    ..28 factors required for epigenetic silencing of Fas, and a select group of other genes, in ras-transformed NIH 3T3 cells. This model system, in conjunction with the experimental reagents we have developed, provides a unique ..
  3. Cellular Retinoic Acid Binding Proteins: Functional Studies
    Lorraine J Gudas; Fiscal Year: 2013
    ..We also propose a comparison of 3T3 cells with H-Ras transformed 3T3 cells to assess how an oncogenic protein perturbs retinoid signaling...
    MICHAEL EDWARD CHARNESS; Fiscal Year: 2013
    ..inhibition of L1 adhesion, and antagonist blockade of ethanol inhibition of cell adhesion using intact NIH/3T3 cells transiently transfected with L1 constructs...
  5. The Role of MTP in Lipid Droplet Formation in Adipocytes
    Larry L Swift; Fiscal Year: 2013
    ..the effects of enhanced MTP expression on lipid droplet formation and maturation in primary pre-adipocytes and 3T3 cells. Using fat-specific MTP knockdown mice, we will define the effects of the knockdown on body weight, body fat ..
  6. Lysyl Oxidase Propeptide: Breast Cancer Inhibitor
    Gail E Sonenshein; Fiscal Year: 2013
    ..as the "ras recision" gene (rrg) with an ability to inhibit the transforming activity of the Ras oncogene in NIH 3T3 cells. Consistently many cancers display reduced LOX gene expression...
  7. Metabolic regulators in the mechanisms of caloric restriction
    Rozalyn M Anderson; Fiscal Year: 2013
    ..To investigate the molecular basis of this, we will use NIH- 3T3 cells to determine: i) the impact of constitutively active or kinase inactive GSK32 on PGC-11 localization, stability ..
  8. Ex Vivo Expansion of Human Limbal Stem Cells for Transplantation
    Sophie Deng; Fiscal Year: 2013
    ..The central hypothesis is that appropriate human feeder cells can replace mouse 3T3 cells to provide a proper microenvironment to support the growth of CESCs, and upon receiving additional appropriate ..
  9. Three dimensional aberration corrected scanning transmission electron microscopy
    David W Piston; Fiscal Year: 2011
    ..Recently, we have obtained 3D images of conventional thin sections containing 3T3 cells showing a lateral resolution of 0.6 nm and an axial resolution of 60 nm...
  10. Building and Validating Location Proteomics Databases
    Robert F Murphy; Fiscal Year: 2011
    ..25,000 independent cell lines expressing GFP protein fusions will be created in NIH 3T3 cells using high-throughput CD-tagging (protein-trapping) methods...
    MICHAEL CHARNESS; Fiscal Year: 1999
    ..type III repeat homology domains (FN3) of L1 exhibit ethanol-sensitive adhesiveness when expressed in NIH/3T3 cells, and will then learn whether specific deletions in the L1 cDNA abolish ethanol's inhibition of Ll-mediated cell-..
    Eliot Spindel; Fiscal Year: 1993
    ..Specifically we propose: (1), To first clone the mouse GRP receptor from Swiss 3T3 cells to take advantage of the high number of receptors expressed by those cells...
    RICHARD BENYA; Fiscal Year: 2001
    ..To evaluate this hypothesis, we will study the murine GRP-R natively expressed by Swiss 3T3 cells and ectopically expressed by Balb 3T3 cells...
    AMOS SMITH; Fiscal Year: 1990
    ..3 They display cytotoxicity against KB and NIH/3T3 cells as well as significant antineoplastic activity in vivo against murine Lewis lung carcinoma...
  15. Cytoplasmic Reactivation of p53 Family Members
    Charles Walker; Fiscal Year: 2004
    ..This phenomenon in clams reflects that seen in a subset of human cancers and is inducible in mouse NIH 3T3 cells. In all of these cases, wild type p53 does not function because it is retained in the cytoplasm...
    Gordon Luk; Fiscal Year: 1992
    ..Normal human fibroblasts, NIH 3T3 cells and human colon and lung carcinoma cell lines will be transfected with the human ODC gene...
    Richard Vallee; Fiscal Year: 1991
    ..The antibodies to the 40,000 molecular weight and HMW MAPs also stain the spindle of mouse 3T3 cells. We plan to continue our isolation of hybridomas to obtain antibodies against other MAPs...
    Ing Ming Chiu; Fiscal Year: 2000
    ..The investigator has previously shown that over-expression of FGF-1 in NIH/3T3 cells confers the recipient cells with a full spectrum of transformed phenotype including tumorigenicity...
    Igor Prudovsky; Fiscal Year: 2005
    ..membrane assay in vivo, completely represses the expression of type I collagen synthesis in vitro and NIH 3T3 cells stably transfected with the soluble form of Jagged1 exhibit type I collagen-dependent chord-like structures ..
    Albert Wong; Fiscal Year: 1993
    ..have cloned the human form of PLC-gamma from a glial tumor and have found that when it is overexpressed in NIH-3T3 cells it can cause transformation...
    Elizabeth Newcomb; Fiscal Year: 1993
    ..of DNA-mediated-gene-transfer was used to introduce high molecular weight DNA from B-CLL cells into mouse NIH 3T3 cells were inoculated into nude mice...
    JERALD HOFFMAN; Fiscal Year: 1980
    ..adenosyltransferase levels and intracellular rates of S-adenosylmethionine synthesis will be measured in mouse 3T3 cells before and after transformation by polyoma virus...
    LLOYD CULP; Fiscal Year: 1993
    ..Untransformed 3T3 cells require two binding activities of FN in an intact polypeptide chain--i.e...
  24. Host Cell Determinants and Retroviral Infection
    Ghalib Alkhatib; Fiscal Year: 2003
    ..on preliminary data generated in our laboratory showing that transfection of a HeLa cell cDNA library into NIH-3T3 cells can render them fusogenic with HTLV-1Env and that co-expression of CD4 resulted in ten fold increase in the cell ..
    Jack Roth; Fiscal Year: 1991
    ..The use of NIH 3T3 cells transfected with high molecular weight DNA from human tumors as an immunogen has resulted in the production of ..
  26. Novel Bioactive Lipid Receptors in Growth Regulation
    Yan Xu; Fiscal Year: 2005
    ..We have recently identified two specific, high-affinity receptors for SPC (OGRI and GPR4). In Swiss 3T3 cells. SPC induces growth inhibition through OGR1 and growth stimulation through GPR4...
    Ken Lin; Fiscal Year: 1990
    ..have shown that several heavy metals including Ni can induce dramatic microtubule (MT) alterations in mouse 3T3 cells. Moreover, dramatic nucleotide pool imbalance, which can influence the fidelity of DNA synthesis, has been ..
    Albert Wong; Fiscal Year: 2001
    ..Overexpression of Gab1 in NIH-3T3 cells results in transformation. Gab1 is overexpressed in primary glial tumors...
    D Lansing Taylor; Fiscal Year: 2000
    ..biological system that will be utilized in this grant period will be the polarized migration of cultured Swiss 3T3 cells during wound healing...
    Vicente Notario; Fiscal Year: 1993
    ..the assumption that the proximity between hamster and mouse cellular backgrounds will allow the detection in NIH/3T3 cells, or nude mice, of cases of malignant alteration of proto-oncogenes that would remain undetectable if they had ..
    Michael Lisanti; Fiscal Year: 2004
    ..We will examine the phenotypic behavior of LGMD-1C mutations of caveolin-3 after heterologous expression in NIH 3T3 cells, as compared with wild-type caveolin-3; 2) To develop transgenic mouse models that over wild-type caveolin-3 and ..
  32. Jak2 involvement in Bcr-Abl oncogenic transformation
    Ralph Arlinghaus; Fiscal Year: 2005
    ..induction through Jak2/Bcr-Abl; 3) Investigate the role of the IL-3/GM-CSF receptors in the conversion of NIH 3T3 cells to a permissive phenotype for oncogenic transformation by BCR-ABL; NIH 3T3 cells are resistant to foci-formation ..
    JAMES PERDUE; Fiscal Year: 1980
    ..properties to somatomedin A stimulates TdR incorporation into the DNA of confluent cultures of CEF and 3T3 cells at a rate that was one-half that achieved with 10 percent fetal calf serum (FCS)...
    Charles Scher; Fiscal Year: 1990
    The treatment of density-arrested BALB/c-3T3 cells with electrophoretically homogenous or highly purified preparations of the platelet-derived growth factor (PDGF) stimulated the rapid and selective accumulation of several species of ..
  35. PPK: Radiation Response and Cell Proliferation
    Peter Stambrook; Fiscal Year: 2006
    ..Curiously, unlike its close relative Plk1, which transforms NIH 3T3 cells following transfection, Plk3 causes mammalian cells to undergo apoptosis following transfection and arrests ..
    Haruo Saito; Fiscal Year: 2001
    ..Expression of the Trio rac1-specific GEF domain also causes anchorage independent growth of NIH 3T3 cells indicating that Trio is a potential proto-oncogene...
    MARY GLICK; Fiscal Year: 1992
    ..In part B we will address the multiantennary oligosaccharides. NIH 3T3 cells which have been transformed by transfection of human tumor DNA will be used...
    Harry Rubin; Fiscal Year: 1992
    Inoculation into nude mice of spontaneously transformed Balb 3T3 cells has revealed that tumor development is a problem of cellular sociobiology, ecology and heritable variation...
    Dihua Yu; Fiscal Year: 2002
    ..that activated c-erbB-2/neu (also known as HER-2) oncogene can lead to higher metastatic potential in mouse 3T3 cells. This result supports the clinical observation that overexpression of the c-erbB-2-encoded p185 receptor is ..
    Dan Mercola; Fiscal Year: 2002
    ..transformed NIH-3T3 and human HT1080 fibrosarcoma cells (7-8) and studies of others with v-raf transformed NIH-3T3 cells (9) have revealed that EGR-1 actually exerts a strong growth and transformation suppressor activity including ..
    Bret Hassel; Fiscal Year: 2001
    ..Expression of transfected RNASE-L renders NIH-3T3 cells sensitive to IF induced growth inhibition in conditions where control cells do not respond; thus, the specific ..
    IGOR TAMM; Fiscal Year: 1990
    ..during the period in which platelet-derived growth factor (PDGF) establishes replicative competence in BALB/c 3T3 cells. IFN treatment inhibits the PDGF-induced synthesis of a prominent nuclear 29 kDa protein (pI) thought to be ..
    DENNIS QUINLAN; Fiscal Year: 1980
    ..However, the uptake of uridine and certain glucose analogs is not reduced. This is in contrast to "normal" 3T3 cells which exhibit decreased transport rates for these same compounds...
    Michael Oldstone; Fiscal Year: 2000
    ..MV is permissive for human and simian but not hamster or murine cells. However, CHO or murine MC57 and 3T3 cells barely permissive to MV become permissive and produce progeny virus when stably expressing any of the four CD46 ..
  45. Indomethacin and p38 Regulating AP-1 In Heat Shock
    Jacob Locke; Fiscal Year: 2004
    ..AP-1 DNA-binding activity via a mechanism involving the redox sensitive signaling factor Ref-1 in HeLa and NIH 3T3 cells; (2) OC-14 peroxide resistant cells overexpress c-Fos, c-Jun, and exhibit elevated AP-1 DNA activity when ..
    JON BACKSTROM; Fiscal Year: 2000
    ..with 5-HT2C receptor antibodies to identify potential phosphorylated regions of receptors expressed in NIH/3T3 cells. Deletion mutants lacking various regions of 5-HT2C receptors will be examined in gel-shift and phosphate-..
  47. Molecular Analysis of Murine Leukemia Virus Entry
    Lorraine Albritton; Fiscal Year: 2007
    ..In addition, XC cells are more sensitive to cholesterol depletion than NIH 3T3 cells, together suggesting that the differences in fusion kinetics may result from lower receptor levels and ..
  48. Novel Inhibitors of Sphingosine Kinase
    KEVIN FRENCH; Fiscal Year: 2004
    ..Importantly, recent studies have demonstrated that SK can act as an oncogene. Overexpression of SK in NIH/3T3 cells results in cellular transformation and allows these cells to grow as tumors...
    Jin Cheng; Fiscal Year: 2001
    ..I recently demonstrated that overexpression of AKT2 can transform NIH 3T3 cells, whereas AKT1 has not been shown to be oncogenic...
    James Thomas; Fiscal Year: 2003
    ..For example, most mesenchymal cells including human dermal fibroblasts, HUVECs and 3T3 cells respond to FGF-1 by vigorous proliferation; synovial cells, however, respond poorly to FGF-1 despite the ..
    Robert Friedman; Fiscal Year: 2003
    ..In NIH 3T3 cells transformed by multiple copies of LTR-C-H-ras (RS485), the transcription of LO is markedly decreased...
  52. The Fnk Kinase and Vascular Cell Growth Control
    JEFFREY WINKLES; Fiscal Year: 2004
    ..We have used a differential display approach to identify FGF-inducible genes in NIH 3T3 cells and vascular cells...
    Robert Tucker; Fiscal Year: 1991
    ..indicated that a transient Cai increase is required for PDGF-stimulated DNA synthesis in quiescent BALB/c 3T3 cells. The present application focuses on defining what other intracellular signals (e.g...
    David Shalloway; Fiscal Year: 1992
    ..kinase, and the nuclear oncogenes adeno E1A, polyoma large T (plt) and myc can induce transformation NIH 3T3 cells and that gap junctional communication is downregulated by overexpression of pp60c-src and activated mutants...
  55. Nutrient Inhibition of Ras-Rho Crosstalk
    Robert Hardy; Fiscal Year: 2007
    ..One recent study indicated that in NIH 3T3 cells oncogenic Ras activates RhoA by inhibiting Ras induced p190Rho-GAP sequestration in a detergent resistant ..
    Shiv Srivastava; Fiscal Year: 1992
    ..genes have been identified in various human malignancies by DNA-mediated gene transfer using mouse NIH/3T3 cells as recipient. The db1 oncogene was detected as a transforming gene of human diffuse B-cell lymphoma...
    Jerry Shay; Fiscal Year: 1992
    We have made NIH/3T3 cells tumorigenic by treatment with the ultimate carcinogen of benzo(a)pyrene, namely r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydro-benzo(a)-pyrene (anti-BPDE) and passaging in culture...
    Martin Lubin; Fiscal Year: 1990
    ..In untransformed mouse NIH-3T3 cells, the efflux pathway opens when cells are in calcium-deficient media, and closes on addition of calcium or an ..
    Dihua Yu; Fiscal Year: 1992
    ..to exert anti-tumor effects in vivo in experimental metastasis assays with the rat neu oncogene transformed 3T3 cells and compare the differences in the frequency of metastases in injected mice with or without the anti-neu ..
  60. Caveolin-1 and Cyclin D1 in Mammary Tumorigenesis
    Richard Pestell; Fiscal Year: 2006
    ..inhibits cellular proliferation and anti-sense caveolin-1 promotes contact independent growth of cultured 3T3 cells. ii) Caveolin-1 inhibits cyclin D1 breast cellular proliferation Cyclin D1 anti-sense can inhibit contact-..
    GEORGE SORENSON; Fiscal Year: 1991
    ..investigate an experimental model of spontaneous metastases from subcutaneous tumors in nude mice induced by NlH/3T3 cells transfected by genomic DNA from a cell line established from metastatic human small cell carcinoma of the lung (..
    Harvey Lodish; Fiscal Year: 1993
    ..We showed that when one receptor polypeptide, H2, is expressed without the other, H1, in 3T3 cells, it is synthesized but rapidly degraded...
    Dan Mercola; Fiscal Year: 2001
    ..investigators have shown that 1) the transcription factor EGR-1 is a potent suppressor of PDGF-B transformed NIH-3T3 cells, 2) activation of c-Jun in certain human tumor lines is associated with constitutively active c-Jun N-terminal ..