cos cells

Summary

Summary: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

Top Publications

  1. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
  2. pmc Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins
    Ethan R Graf
    Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Cell 119:1013-26. 2004
  3. ncbi Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor
    D Wang
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 105:851-62. 2001
  4. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
  5. ncbi Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins
    Toshiki Itoh
    Department of Cell Biology and Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Dev Cell 9:791-804. 2005
  6. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
  7. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
  8. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
  9. ncbi Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
  10. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001

Research Grants

Detail Information

Publications381 found, 100 shown here

  1. ncbi An improved cyan fluorescent protein variant useful for FRET
    Mark A Rizzo
    Nat Biotechnol 22:445-9. 2004
    ..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
  2. pmc Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins
    Ethan R Graf
    Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO 63110, USA
    Cell 119:1013-26. 2004
    ....
  3. ncbi Activation of cardiac gene expression by myocardin, a transcriptional cofactor for serum response factor
    D Wang
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 105:851-62. 2001
    ..Myocardin is the founding member of a class of muscle transcription factors and provides a mechanism whereby SRF can convey myogenic activity to cardiac muscle genes...
  4. ncbi A class of membrane proteins shaping the tubular endoplasmic reticulum
    Gia K Voeltz
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 124:573-86. 2006
    ..The simultaneous absence of the reticulons and Yop1p in S. cerevisiae results in disrupted tubular ER. We propose that these "morphogenic" proteins partition into and stabilize highly curved ER membrane tubules...
  5. ncbi Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins
    Toshiki Itoh
    Department of Cell Biology and Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Dev Cell 9:791-804. 2005
    ..These results suggest a close interplay between the mechanisms that control actin dynamics and those that mediate plasma membrane invagination and fission...
  6. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  7. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
    ..Remarkably, inhibition of Drp1 blocks cell death, implicating mitochondrial fission as an important step in apoptosis...
  8. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses...
  9. ncbi Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
    ..We propose that similar mechanisms underlie the specific association of CLIPs and EB1-related proteins with the ends of growing microtubules in non-neuronal and neuronal cells...
  10. pmc Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding
    D Michaelson
    Department of Medicine, New York University School of Medicine, New York, New York 10016, USA
    J Cell Biol 152:111-26. 2001
    ..Thus, despite the common essential feature of the CAAX (prenylation, AAX tripeptide proteolysis, and carboxyl methylation) motif, the subcellular localizations of Rho GTPases, like their functions, are diverse and dynamic...
  11. pmc FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets
    Naonobu Nishino
    Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan
    J Clin Invest 118:2808-21. 2008
    ..small lipid droplets, increased lipolysis, and decreased triacylglycerol storage, while expression of FSP27 in COS cells promoted the formation of large lipid droplets...
  12. pmc Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells
    Reina E Itoh
    Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, CREST, Japan
    Mol Cell Biol 22:6582-91. 2002
    ..In conclusion, use of these single-molecule probes to determine Rac and Cdc42 activity will accelerate the analysis of the spatiotemporal regulation of Rac and Cdc42 in a living cell...
  13. ncbi Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration
    Takashi Watanabe
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Dev Cell 7:871-83. 2004
    ....
  14. ncbi Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
    B Wolff
    Sandoz Research Institute, Brunner Strasse 59, A1230 Vienna, Austria
    Chem Biol 4:139-47. 1997
    ..Translocation of Rev from the nucleus to the cytoplasm is essential if Rev is to function. We wanted to identify inhibitors of this transport process because they would be potential antiviral agents...
  15. ncbi Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms
    L Chen
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
    Nature 408:936-43. 2000
    ....
  16. pmc A clathrin-dependent pathway leads to KRas signaling on late endosomes en route to lysosomes
    Albert Lu
    Departament de Biologia Cellular, Immunologia i Neurociencies, Institut d Investigacions Biomediques August Pi i Sunyer, Serveis Cientificotecnics, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
    J Cell Biol 184:863-79. 2009
    ..Altogether, this study reveals novel aspects about KRas intracellular trafficking and signaling, shedding new light on the mechanisms controlling Ras regulation in the cell...
  17. doi An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio
    Yutaka Kodama
    Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA
    Biotechniques 49:793-805. 2010
    ..This mutation allows a 4-fold increase in the S/N ratio of the BiFC assay in living cells. This improved Venus-based BiFC system will facilitate PPI studies in various biological research fields...
  18. ncbi Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells
    Oleg O Glebov
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 8:46-54. 2006
    ..We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells...
  19. pmc The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors
    J B Hogenesch
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 95:5474-9. 1998
    ..Finally, we found that MOP3 mRNA expression overlaps in a number of tissues with each of its four potential partner molecules in vivo...
  20. ncbi Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin
    S Naisbitt
    Howard Hughes Medical Institute, Department of Neurobiology, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA
    Neuron 23:569-82. 1999
    ..Shank may function as a scaffold protein in the PSD, potentially cross-linking NMDA receptor/PSD-95 complexes and coupling them to regulators of the actin cytoskeleton...
  21. ncbi CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
    ..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends...
  22. ncbi Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance
    K Brose
    Department of Anatomy, Howard Hughes Medical Institute, University of California, San Francisco 94143 0452, USA
    Cell 96:795-806. 1999
    ..These results support the hypothesis that Slit proteins have an evolutionarily conserved role in axon guidance as repulsive ligands for Robo receptors...
  23. ncbi Imaging of lipid biosynthesis: how a neutral lipid enters lipid droplets
    Lars Kuerschner
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr 108, D 01307 Dresden, Germany
    Traffic 9:338-52. 2008
    ..In vitro assays on purified lipid droplets show that this fraction of the biosynthetic TG is directly inserted into the growing droplet...
  24. pmc TREK-1, a K+ channel involved in neuroprotection and general anesthesia
    C Heurteaux
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, Institut Paul Hamel, Sophia Antipolis, Valbonne, France
    EMBO J 23:2684-95. 2004
    ..Trek1-/- mice are also resistant to anesthesia by volatile anesthetics. TREK-1 emerges as a potential innovative target for developing new therapeutic agents for neurology and anesthesiology...
  25. ncbi Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
    Akin Akinc
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Gene Med 7:657-63. 2005
    ..According to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosomes, resulting in the release of the vector into the cytoplasm...
  26. ncbi beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor
    Sudha K Shenoy
    Howard Hughes Medical Institute at Duke University Medical Center, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 281:1261-73. 2006
    ..These findings demonstrate that the beta2AR can signal to ERK via a GRK5/6-beta-arrestin-dependent pathway, which is independent of G protein coupling...
  27. ncbi Regulation of androgen receptor activity by tyrosine phosphorylation
    Zhiyong Guo
    Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Cancer Cell 10:309-19. 2006
    ....
  28. pmc Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells
    Peter Varnai
    Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 175:377-82. 2006
    ....
  29. ncbi Ras signalling on the endoplasmic reticulum and the Golgi
    Vi K Chiu
    Department of Medicine, Cell Biology and Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Nat Cell Biol 4:343-50. 2002
    ..Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane...
  30. ncbi Neuroligin 2 is exclusively localized to inhibitory synapses
    Frederique Varoqueaux
    Max Planck Institute for Experimental Medicine, Department of Molecular Neurobiology, Gottingen, Germany
    Eur J Cell Biol 83:449-56. 2004
    ..Our findings identify neuroligin 2 as a new cell adhesion protein specific for inhibitory synapses and open new avenues for identifiying the constituents of this unique type of postsynaptic specialization...
  31. ncbi Smurf1 interacts with transforming growth factor-beta type I receptor through Smad7 and induces receptor degradation
    T Ebisawa
    Department of Biochemistry, The Cancer Institute of the Japanese Foundation for Cancer Research, and Research for the Future Program, the Japan Society for the Promotion of Science, 1 37 1 Kami Ikebukuro, Toshima ku, Tokyo 170 8455, Japan
    J Biol Chem 276:12477-80. 2001
    ..These results thus reveal a novel function of Smad7, i.e. induction of degradation of TbetaR-I through recruitment of an E3 ligase to the receptor...
  32. ncbi S. typhimurium encodes an activator of Rho GTPases that induces membrane ruffling and nuclear responses in host cells
    W D Hardt
    Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, 11794 5222, USA
    Cell 93:815-26. 1998
    ..These findings establish a paradigm for microbial stimulation of cellular responses in which the pathogen induces signaling events by directly engaging the signaling machinery within the host cell...
  33. ncbi Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi
    Ying Jie Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
    Cell 114:299-310. 2003
    ..We propose that PI4KIIalpha establishes the Golgi's unique lipid-defined organelle identity by generating PI(4)P-rich domains that specify the docking of the AP-1 coat machinery...
  34. pmc Covalent modification of the androgen receptor by small ubiquitin-like modifier 1 (SUMO-1)
    H Poukka
    Department of Physiology, Institute of Biomedicine, and Department of Clinical Chemistry, University of Helsinki, FIN 00014, Helsinki, Finland
    Proc Natl Acad Sci U S A 97:14145-50. 2000
    ..Taken together, our data suggest that reversible sumoylation is a mechanism for regulation of steroid receptor function...
  35. ncbi Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics
    D C Edwards
    Department of Chemistry, University of California at San Diego, La Jolla 92093 0650, USA
    Nat Cell Biol 1:253-9. 1999
    ..A Pak1-specific inhibitor, corresponding to the Pak1 autoinhibitory domain, blocks LIM-kinase-induced cytoskeletal changes. Activated GTPases can thus regulate actin depolymerization through Pak1 and LIM-kinase...
  36. pmc Hereditary spastic paraplegia proteins REEP1, spastin, and atlastin-1 coordinate microtubule interactions with the tubular ER network
    Seong H Park
    Cellular Neurology Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke NINDS, NIH, Bethesda, Maryland 20892 3738, USA
    J Clin Invest 120:1097-110. 2010
    ..Thus, defects in tubular ER shaping and network interactions with the microtubule cytoskeleton seem to be the predominant pathogenic mechanism of HSP...
  37. doi Enhanced delivery and expression of a nanoencapsulated DNA vaccine vector for respiratory syncytial virus
    Seyhan Boyoglu
    Center for NanoBiotechnology Research, Alabama State University, Montgomery, Alabama, USA
    Nanomedicine 5:463-72. 2009
    ..Immunohistochemical and real-time polymerase chain reaction results showed a higher level of RSV protein expression in mouse tissues given when DCNPs were injected intravenously as compared with naked DNA...
  38. pmc Eph receptor function is modulated by heterooligomerization of A and B type Eph receptors
    Peter W Janes
    Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia
    J Cell Biol 195:1033-45. 2011
    ..Importantly, they imply that in tumor cells coexpressing different Ephs, functional mutations in one subtype may cause phenotypes that are a result of altered signaling from heterotypic rather from homotypic Eph clusters...
  39. ncbi Spatio-temporal images of growth-factor-induced activation of Ras and Rap1
    N Mochizuki
    Department of Structural Analysis, National Cardiovascular Center Research Institute, 5 7 1 Fujishirodai, Suita Shi, Osaka 565 8565, Japan
    Nature 411:1065-8. 2001
    ..These observations may resolve long-standing questions as to how Ras and Rap1 induce different cellular responses and how the signals for differentiation and survival are distinguished by neuronal cells...
  40. ncbi Reelin is a ligand for lipoprotein receptors
    G D'ARCANGELO
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
    Neuron 24:471-9. 1999
    ..In dissociated neurons, apoE reduces the level of Reelin-induced tyrosine phosphorylation of Dab1. These data suggest that Reelin directs neuronal migration by binding to VLDLR and ApoER2...
  41. pmc The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15
    A S Sengar
    Programs of Cancer and Blood Research, and Developmental Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada
    EMBO J 18:1159-71. 1999
    ..The Ese1-Eps15 scaffold therefore links dynamin, epsin and other endocytic pathway components...
  42. ncbi Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170
    Masaki Fukata
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Cell 109:873-85. 2002
    ..These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization...
  43. pmc A family of Rhomboid intramembrane proteases activates all Drosophila membrane-tethered EGF ligands
    Sinisa Urban
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    EMBO J 21:4277-86. 2002
    ..This is distinct from the metalloprotease-induced activation of mammalian EGF-like ligands...
  44. doi Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes
    Mike Heilemann
    Angewandte Laserphysik and Laserspektroskopie, Universitat Bielefeld, Universitatsstrasse 25, 33615 Bielefeld, Germany
    Angew Chem Int Ed Engl 47:6172-6. 2008
  45. ncbi PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1
    Takashi Nishimura
    Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Nat Cell Biol 7:270-7. 2005
    ..We propose that the PAR-6-PAR-3 complex mediates Cdc42-induced Rac activation by means of STEF/Tiam1, and that this process seems to be required for the establishment of neuronal polarity...
  46. ncbi Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation
    Chang Deng Hu
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Cell 9:789-98. 2002
    ..These results attest to the general applicability of the BiFC assay for studies of protein interactions...
  47. pmc Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules
    Nancy Kedersha
    Division of Rheumatology and Immunology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Mol Biol Cell 13:195-210. 2002
    ..We discuss these results in the context of a translational checkpoint model wherein TIA and eIF2 are functional antagonists of translational initiation, and in which lack of ternary complex drives SG assembly...
  48. pmc The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes
    Patrick D Allaire
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Quebec H3A 2B4, Canada
    Mol Cell 37:370-82. 2010
    ..Our data reveal an enzymatic activity for a DENN domain and demonstrate that distinct Rab GTPases can recruit a common protein machinery to various sites within the endosomal network to establish cargo-selective recycling pathways...
  49. pmc Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses
    J Y Song
    Max Planck Institut fur experimentelle Medizin, Abteilung Molekulare Neurobiologie, Hermann Rein Strasse 3, D 37075 Gottingen, Germany
    Proc Natl Acad Sci U S A 96:1100-5. 1999
    ..In addition, the neuroligin/beta-neurexin junction may be involved in the specification of excitatory synapses...
  50. doi Synaptic imbalance, stereotypies, and impaired social interactions in mice with altered neuroligin 2 expression
    Rochelle M Hines
    Department of Psychiatry, Brain Research Centre, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    J Neurosci 28:6055-67. 2008
    ..These findings may provide the neural basis for E/I imbalance and altered behavior associated with neurodevelopmental disorders...
  51. pmc Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate
    Roberto Zoncu
    Department of Cell Biology and Howard Hughes Medical Institute, Kavli Institute For Neuroscience and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 104:3793-8. 2007
    ..These findings demonstrate the critical importance of PI(4,5)P(2) in clathrin coat dynamics and Arp2/3-dependent actin regulation...
  52. ncbi Neuroprotection in ischemia: blocking calcium-permeable acid-sensing ion channels
    Zhi Gang Xiong
    Robert S Dow Neurobiology Laboratories, Legacy Research, Portland, OR 97232, USA
    Cell 118:687-98. 2004
    ..Thus, acidosis injures the brain via membrane receptor-based mechanisms with resultant toxicity of [Ca2+]i, disclosing new potential therapeutic targets for stroke...
  53. ncbi A ligand-gated association between cytoplasmic domains of UNC5 and DCC family receptors converts netrin-induced growth cone attraction to repulsion
    K Hong
    Department of Biology, University of California, San Diego, 92093, USA
    Cell 97:927-41. 1999
    ....
  54. pmc Targeting and activation of Rac1 are mediated by the exchange factor beta-Pix
    Jean Paul ten Klooster
    Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, Netherlands
    J Cell Biol 172:759-69. 2006
    ..These data provide a model for the intracellular targeting and localized activation of Rac1 through its exchange factor beta-Pix...
  55. doi ALS-linked P56S-VAPB, an aggregated loss-of-function mutant of VAPB, predisposes motor neurons to ER stress-related death by inducing aggregation of co-expressed wild-type VAPB
    Hiroaki Suzuki
    Department of Cell Biology and Neuroscience, Keio University School of Medicine, Tokyo, Japan
    J Neurochem 108:973-985. 2009
    ..These results lead us to hypothesize that the total loss of VAPB function in unfolded protein response, induced by one P56S mutant allele, may contribute to the development of P56SVAPB- induced amyotrophic lateral sclerosis...
  56. pmc Steroid-induced androgen receptor-oestradiol receptor beta-Src complex triggers prostate cancer cell proliferation
    A Migliaccio
    Istituto di Patologia Generale e Oncologia, Facolta di Medicina e Chirurgia, II Universita di Napoli, Largo S Aniello a Caponapoli 2, 80138 Napoli, Italy
    EMBO J 19:5406-17. 2000
    ..Data from transfected Cos cells confirm and extend the findings from these cells...
  57. ncbi Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing
    Chi Zhang
    Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 278:35325-36. 2003
    ....
  58. ncbi A schizophrenia-associated mutation of DISC1 perturbs cerebral cortex development
    Atsushi Kamiya
    Department of Psychiatry Neurobiology, Johns Hopkins University School of Medicine, 600 N Wolfe Street, CMSC 8 117, Baltimore, MD 21287, USA
    Nat Cell Biol 7:1167-78. 2005
    ..These results indicate that DISC1 is involved in cerebral cortex development, and suggest that loss of DISC1 function may underlie neurodevelopmental dysfunction in schizophrenia...
  59. ncbi A novel immunoglobulin superfamily receptor for cellular and viral MHC class I molecules
    D Cosman
    Immunex Corporation, Seattle, Washington 98101, USA
    Immunity 7:273-82. 1997
    ..Upon tyrosine phosphorylation, LIR-1 associates with the tyrosine phosphatase SHP-1. In contrast to KIRs, LIR-1 is expressed predominantly on monocytic and B lymphoid cell types, suggesting a distinct biological function...
  60. ncbi Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes
    M G Ford
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Science 291:1051-5. 2001
    ..This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed...
  61. doi Phosphorylation of CLASP2 by GSK-3beta regulates its interaction with IQGAP1, EB1 and microtubules
    Takashi Watanabe
    Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, Nagoya, Aichi, Japan
    J Cell Sci 122:2969-79. 2009
    ..Thus, phosphorylation of CLASP2 by GSK-3beta appears to control the regional linkage of microtubules to actin filaments through IQGAP1 for cell migration...
  62. ncbi Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 2XY, UK
    Nat Genet 36:585-95. 2004
    ..Our data provide proof-of-principle for the potential of inducing autophagy to treat Huntington disease...
  63. ncbi Identification of SopE2 from Salmonella typhimurium, a conserved guanine nucleotide exchange factor for Cdc42 of the host cell
    S Stender
    Max von Pettenkofer Institut, Munich, Germany
    Mol Microbiol 36:1206-21. 2000
    ..In conclusion, as a result of SopE2 all S. typhimurium strains tested have the capacity to activate Cdc42 signalling inside host cells which is important to ensure efficient entry...
  64. ncbi Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors
    T Takahashi
    Department of Neurology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cell 99:59-69. 1999
    ..Sema3A treatment leads to the redistribution of growth cone NP-1 and plexin into clusters. Thus, physiologic Sema3A receptors consist of NP-1/plexin complexes...
  65. pmc The RasGAP-associated endoribonuclease G3BP assembles stress granules
    Hélène Tourrière
    Institut de Genetique Moleculaire de Montpellier, UMR 5535 du CNRS, IFR 122, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France
    J Cell Biol 160:823-31. 2003
    ..These results suggest that G3BP is an effector of SG assembly, and that Ras signaling contributes to this process by regulating G3BP dephosphorylation...
  66. ncbi Identification of PSD-95 palmitoylating enzymes
    Masaki Fukata
    Department of Physiology, University of California at San Francisco, San Francisco, California 94143, USA
    Neuron 44:987-96. 2004
    ..This study suggests that P-PATs regulate synaptic function through PSD-95 palmitoylation...
  67. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ....
  68. doi Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells
    Sarah Dunn
    Institute for Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    J Cell Sci 121:1085-95. 2008
    ..These data suggest that kinesin-1 preferentially binds to and trafficks on detyrosinated microtubules in vivo, providing a potential basis for the spatial targeting of kinesin-1-based cargo transport...
  69. pmc Interaction of reelin with amyloid precursor protein promotes neurite outgrowth
    HYANG SOOK HOE
    Department of Neuroscience, Georgetown University Medical Center, Washington, DC 20057 1464, USA
    J Neurosci 29:7459-73. 2009
    ..Addition of an alpha3beta1 integrin antibody prevented APP and Reelin-induced neurite outgrowth. These findings demonstrate that Reelin interacts with APP, potentially having important effects on neurite development...
  70. pmc Electron microscopy of whole cells in liquid with nanometer resolution
    N de Jonge
    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232 0615, USA
    Proc Natl Acad Sci U S A 106:2159-64. 2009
    ..The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods...
  71. pmc Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition
    Jennetta W Hammond
    Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA
    PLoS Biol 7:e72. 2009
    ..However, only dimeric motors undergo ATP-dependent processive motility. Thus, KIF1A may be uniquely suited to use both diffuse and processive motility to drive long-distance transport in neuronal cells...
  72. ncbi Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy
    Anne Sophie Nicot
    Institut de Genetique et de Biologie Moleculaire et Cellulaire IGBMC, Department of Molecular Pathology, F 67400 Illkirch, France
    Nat Genet 39:1134-9. 2007
    ....
  73. pmc Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST
    A J Garton
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 2208, USA
    Mol Cell Biol 16:6408-18. 1996
    ..This exclusive association was observed in lysates from several cell lines and in transfected COS cells, but was not observed with other members of the PTP family, strongly suggesting that p130(cas) represents a ..
  74. ncbi Novel 5 alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer
    Motohide Uemura
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Shirokanedai 4 6 1 Minato ku, Tokyo 108 8639, Japan
    Cancer Sci 99:81-6. 2008
    ....
  75. pmc Identification of a novel Bves function: regulation of vesicular transport
    Hillary A Hager
    The Stahlman Cardiovascular Research Laboratories, Program for Developmental Biology, and Department of Medicine, Vanderbilt University, Medical Center, Nashville, TN 37232, USA
    EMBO J 29:532-45. 2010
    ..Thus, this study establishes a newly identified role for Bves in vesicular transport and reveals a novel, broadly applied mechanism governing SNARE protein function...
  76. pmc EB1 and EB3 control CLIP dissociation from the ends of growing microtubules
    Yulia Komarova
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA
    Mol Biol Cell 16:5334-45. 2005
    ....
  77. ncbi Regulation of dendritic spine morphology by SPAR, a PSD-95-associated RapGAP
    D T Pak
    Department of Neurobiology, Howard Hughes Medical Institute, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Neuron 31:289-303. 2001
    ..The effects of SPAR on spine morphology depend on the RapGAP and actin-interacting domains, implicating Rap signaling in the regulation of postsynaptic structure...
  78. ncbi Bst-2/HM1.24 is a raft-associated apical membrane protein with an unusual topology
    Sabine Kupzig
    Department of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
    Traffic 4:694-709. 2003
    ..Data from several experiments are consistent with this localization. We present a model in which BST-2/HM1.24 serves to link adjacent lipid rafts within the plasma membrane...
  79. pmc Movement of Bax from the cytosol to mitochondria during apoptosis
    K G Wolter
    Biochemistry Section, Surgical Neurology Branch, Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 139:1281-92. 1997
    ....
  80. pmc Regulation of cardiac gene expression by KLF15, a repressor of myocardin activity
    Joost J Leenders
    Heart Failure Research Center, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Biol Chem 285:27449-56. 2010
    ..This uncovers a novel mechanism where activated p38 MAPK decreases KLF15, an important constitutive transcriptional repressor whose removal seems a vital step to allow the induction of pathological LVH...
  81. ncbi The MAD-related protein Smad7 associates with the TGFbeta receptor and functions as an antagonist of TGFbeta signaling
    H Hayashi
    Division of Gastroenterology, The Hospital for Sick Children, Toronto, Ontario, Canada
    Cell 89:1165-73. 1997
    ..These studies thus define a novel function for MAD-related proteins as intracellular antagonists of the type I kinase domain of TGFbeta family receptors...
  82. ncbi Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    S M Elbashir
    Department of Cellular Biochemistry, Max-Planck-Institute for Biophysical Chemistry, , Germany
    Nature 411:494-8. 2001
    ..Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics...
  83. pmc Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin
    M M Kessels
    Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, D 39008 Magdeburg, Germany
    J Cell Biol 153:351-66. 2001
    ....
  84. pmc Clathrin-independent endocytosis: a unique platform for cell signaling and PM remodeling
    Julie G Donaldson
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Signal 21:1-6. 2009
    ..In this review we focus on studies that have been performed using HeLa and COS cells as model systems for understanding this membrane trafficking system...
  85. pmc Isoforms of steroid receptor co-activator 1 differ in their ability to potentiate transcription by the oestrogen receptor
    E Kalkhoven
    Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    EMBO J 17:232-43. 1998
    ..Thus, SRC1 exists as functionally distinct isoforms which are likely to play different roles in ER-mediated transcription...
  86. pmc Polymorphism in the Plasmodium falciparum erythrocyte-binding ligand JESEBL/EBA-181 alters its receptor specificity
    D C Ghislaine Mayer
    Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 4 Center Drive, Room 4126, Bethesda, MD 20892 0425, USA
    Proc Natl Acad Sci U S A 101:2518-23. 2004
    ..falciparum may have been successful in endemic areas because it has mutated the ligands of the DBL family to create multiple pathways of invasion, thus making selection of refractory erythrocytes unlikely...
  87. ncbi Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
    M A Sells
    Fox Chase Cancer Centre, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Curr Biol 7:202-10. 1997
    ..This kinase is therefore a candidate effector for the changes in cell shape induced by growth factors...
  88. ncbi Novel, isotype-specific sensors for protein kinase A subunit interaction based on bioluminescence resonance energy transfer (BRET)
    Anke Prinz
    University of Kassel, Department of Biochemistry, Heinrich Plett Str 40, D 34132 Kassel, Germany
    Cell Signal 18:1616-25. 2006
    ....
  89. pmc Two distinct phosphoinositide 3-kinases mediate polypeptide growth factor-stimulated PKB activation
    Alexandre Arcaro
    Lung Cancer Biology Group, Division of Medicine, Imperial College Faculty of Medicine, Du Cane Road, London W12 0NN, UK
    EMBO J 21:5097-108. 2002
    ..A dominant-negative PI3KC2beta blocked both PKB activation and SCLC cell growth in response to SCF. Together our data provide novel insights into the specificity and functional significance of PI3K signalling in human cancer...
  90. pmc Live-cell imaging reveals divergent intracellular dynamics of polyglutamine disease proteins and supports a sequestration model of pathogenesis
    Yaohui Chai
    Department of Neurology, 3160 Medical Labs, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    Proc Natl Acad Sci U S A 99:9310-5. 2002
    ....
  91. ncbi Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins
    C Serra-Pages
    Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 273:15611-20. 1998
    ..We propose that liprins function to localize LAR family tyrosine phosphatases at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates...
  92. ncbi Tip60 and histone deacetylase 1 regulate androgen receptor activity through changes to the acetylation status of the receptor
    Luke Gaughan
    Prostate Research Group, School of Surgical Sciences, University of Newcastle upon Tyne, Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom
    J Biol Chem 277:25904-13. 2002
    ....
  93. ncbi Regulated intracellular ligand transport and proteolysis control EGF signal activation in Drosophila
    J R Lee
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Cell 107:161-71. 2001
    ..Rhomboid-1 is localized in the Golgi, where it promotes the cleavage of Spitz. This defines a novel growth factor release mechanism that is distinct from metalloprotease-dependent shedding from the cell surface...
  94. pmc Dynamics of putative raft-associated proteins at the cell surface
    Anne K Kenworthy
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T, Rm 101, 18 Library Dr, Bethesda, MD 20895, USA
    J Cell Biol 165:735-46. 2004
    ..Thus, raft association is not the dominant factor in determining long-range protein mobility at the cell surface...
  95. ncbi Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase
    M Maekawa
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8315, Japan
    Science 285:895-8. 1999
    ..These results indicate that phosphorylation of LIM-kinase by ROCK and consequently increased phosphorylation of cofilin by LIM-kinase contribute to Rho-induced reorganization of the actin cytoskeleton...
  96. ncbi Ataxin-3 binds VCP/p97 and regulates retrotranslocation of ERAD substrates
    Xiaoyan Zhong
    Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Hum Mol Genet 15:2409-20. 2006
    ..These data suggest that a normal function of AT3 is to regulate flow through the ERAD pathway by modulating VCP-dependent extraction of proteins from the ER...
  97. ncbi Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions
    Y John Shyu
    Purdue University, West Lafayette IN 47907, USA
    Biotechniques 40:61-6. 2006
    ..These newly identified fluorescent protein fragments will facilitate the study of protein-protein interactions in living cells and whole animals under physiological conditions...
  98. ncbi RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex
    Nobuaki Kobayashi
    Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama City 236 0004, Japan
    Gene 396:236-47. 2007
    ..Finally, we confirmed the cellular localization of these proteins. Taken together, our results reveal that RanBPM, ARMC8alpha, ARMC8beta, Muskelin, p48EMLP, and p44CTLH form complexes in cells...
  99. pmc Dual roles for the Drosophila PI 4-kinase four wheel drive in localizing Rab11 during cytokinesis
    Gordon Polevoy
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1L7, Canada
    J Cell Biol 187:847-58. 2009
    ..Moreover, activated Rab11 partially suppresses loss of fwd. Our data suggest Fwd plays catalytic and noncatalytic roles in regulating Rab11 during cytokinesis...
  100. pmc Mammalian Golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes
    C C Hoogenraad
    MGC Department of Cell Biology, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    EMBO J 20:4041-54. 2001
    ..Taken together, these data suggest that mammalian BICD2 plays a role in the dynein- dynactin interaction on the surface of membranous organelles, by associating with these complexes...
  101. pmc TREK-1 is a heat-activated background K(+) channel
    F Maingret
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS UPR 411, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
    EMBO J 19:2483-91. 2000
    ..Expression of TREK-1 in peripheral sensory neurons as well as in central hypothalamic neurons makes this K(+) channel an ideal candidate as a physiological thermoreceptor...

Research Grants64

  1. Sodium Chloride Cotransporter Regulation by WNK Kinase
    Hui Cai; Fiscal Year: 2010
    ....
  2. Nox4 and Vascular Homeostasis
    John F Keaney; Fiscal Year: 2013
    ..With this information in hand, we should have the requisite insight to design new tools directed at modulating vascular phenotype with an eye toward the treatment of vascular disease. ..
  3. Molecular Regulation of Cardiac KATP Channels in Ischemia
    Jonathan C Makielski; Fiscal Year: 2013
    ..The results may suggest more specific therapeutic targets in the SUR2 variants, and it will also provide important new information on mitochondrial structure and function in heart. ..
  4. Molecular Regulation of Cardiac KATP Channels in Ischemia
    Jonathan C Makielski; Fiscal Year: 2012
    ..The results may suggest more specific therapeutic targets in the SUR2 variants, and it will also provide important new information on mitochondrial structure and function in heart. ..
  5. Enzymatic and motor properties of myosin III
    Christopher M Yengo; Fiscal Year: 2012
    ..The biochemically characterized constructs will be examined in cultured HeLa/COS cells, photoreceptors, and inner ear hair cells to determine how the activity of myosin III mediates its cellular ..
  6. Leukocyte Transendothelial Migration: Coordination of Rho GTPase Signaling
    CHRISTOPHER M WELCH; Fiscal Year: 2012
    ....
  7. Sub-Wavelength Imaging of Intracellular Metal Ions
    Joseph R Lakowicz; Fiscal Year: 2010
    ..We selected the different cell lines for imaging, including COS cells, 293 cells, PC12 cells and mitochondria therein...
  8. ATP-SENSITIVE POTASSIUM CHANNELS AND INSULIN SECRETION
    Show Ling Shyng; Fiscal Year: 2013
    ..identify and characterize novel pharmacological chaperones that rescue trafficking-impaired KATP channels in COS cells and rodent and human islets/[unreadable]-cells...
  9. STRUCTURE/FUNCTION OF SHORT WAVELENGTH VISUAL PIGMENTS
    Barry E Knox; Fiscal Year: 2011
    ..In our First Aim, the short wavelength-sensitive (SWS1) visual pigments will be purified from transfected COS cells in order to investigate how particular amino acid residues in the transmembrane helical segments and the ..
  10. VITAMIN D HORMONE MECHANISM OF ACTION IN CULTURED CELLS
    MARK HAUSSLER; Fiscal Year: 1999
    ....
  11. HUMAN IL3 ACTIVATION OF PROTEIN KINASE C AND BCL-2 GENE
    Yufang Shi; Fiscal Year: 2001
    ..The affinity constant of the receptor transfected into COS cells will be compared to that of the natural receptor...
  12. LAMININ GENE EXPRESSION IN GLOMERULAR CELLS
    Karol Bomsztyk; Fiscal Year: 2002
    ..If the silencer can been narrowed down to 10-20 bp, then mutations can be made in the context of the -233/-15 fragment to abolish silencer activity. He will also test the silencer effect on heterologous promoters (SV40). ..
  13. Lipid modulation of beta-cell calcium channels
    Lina Moitoso de Vargas; Fiscal Year: 2006
    ..We will separate the effects of FFA on different L-type VDCC and determine the effects of FFA on currents through mutant channels generated in Aim 2. ..
  14. Development of an Optimized System for Non-covalent Delivery of Proteins into Cel
    Kevin Burgess; Fiscal Year: 2012
    ..This would be the first robust reagent of its kind, and would open the doors to single molecule tracking of proteins in living cells. ..
  15. BIOSYNTHESIS AND GENETICS OF GLYCOPROTEINS IIB AND IIIA
    Marc Shuman; Fiscal Year: 1993
    ..Normal and mutant mRNA will be microinjected into frog oocytes or COS cells. The effects of each mutation on assembly of the GPIIb-IIIa complex and on 125I- RGDS, 125I-gamma chain ..
  16. CR1 Polymorphisms and Susceptibility to Severe Malaria
    JOANN MOULDS; Fiscal Year: 2002
    ..while those negative for a Knops blood group antigen, Sla, formed fewer rosettes using PfEMP-1 expressing COS cells. Studies have shown that antibodies to rosettes are acquired with age and malaria immunity...
  17. STRUCTURE AND FUNCTION OF THE HSV DNA POLYMERASE
    DAVID DORSKY; Fiscal Year: 1993
    ..The in vitro translated pol and mutants will also be used as targets for affinity-labeling by photoactive nucleotide analogs and specifically designed small DNA fragments...
  18. MAXADILAN, A CUTANEOUS VASODILATOR FROM SAND FLIES
    Ethan Lerner; Fiscal Year: 2002
    ..of cDNA libraries derived from rabbit aorta or a cell line to which maxadilan binds, will be performed in COS cells in order to obtain the gene encoding the maxadilan receptor...
  19. HUMORAL REGULATION OF NORMAL AND MALIGNANT HEMATOPOIESIS
    Thomas Kelly; Fiscal Year: 2004
    ..hemopoietins to be studied include GC-CSF, IL-3 and IL-5, and the model systems involve Xenopus oocytes, monkey COS cells, human leukemia HL-60 cells, as well as normal human neutrophils and mononuclear phagocytes, and their leukemic ..
  20. MOLECULAR ANALYSIS OF INSP3 RECEPTOR STRUCTURE FUNCTION
    Gregory Mignery; Fiscal Year: 1999
    ..assembly into the tetrameric structure will be elucidated by expressing mutagenized receptor constructs into COS cells and assessing subunit assembly by sucrose gradient sedimentation and chemical cross linking. 3...
  21. CYTOSOLIC PHOSPHOLIPASE A2 TRANSFORMED CELLS
    Raphael Nemenoff; Fiscal Year: 2002
    ..immunoprecipitate cPLA2, and obtained the cDNA for the human homolog of the enzyme, which when expressed in COS cells, results in induction of PLA2 activity...
  22. DYNORPHIN AND BETA CELL SENSITIZATION
    MARVIN GERSHENGORN; Fiscal Year: 1999
    ....
  23. BIOLOGY & BIOCHEMISTRY OF MEMBRANE INTERLEUKIN 1
    Evelyn Kurt Jones; Fiscal Year: 1992
    ..The specificity of these responses will be determined by blocking studies with anti-IL-1 antibodies...
  24. RENAL DOPAMINE RECEPTORS AND INHIBITION OF NA,K-ATPASE
    Istvan Seri; Fiscal Year: 1993
    ....
  25. Role of cockroach proteases in airway inflammation
    Kristen Page; Fiscal Year: 2009
    ..Finally, we will investigate the role of endogenous TNF alpha in the airways using a TNF alpha neutralizing antibody and TNF alpha knockout mice. ..
  26. The Role of N1-Inosine Adducts in Butadiene Mutagenesis
    Gunnar Boysen; Fiscal Year: 2005
    ..coli and COS cells. There is no information on the formation, persistence or repair of these adducts in vitro or in vivo...
  27. Regulation of the MAP kinase pathway in the CD4+ T cells
    Binfeng Lu; Fiscal Year: 2007
    ..Aim 2. Study the role of GADD45 protein in mediating the activation of the p38/JNK MAP kinase pathway by cytokines. Aim 3. Study the effect of mutation of GADD45gamma, GADD45beta, or MEKK4 in murine collagen induced arthritis. ..
  28. PLATELETS IN RHEUMATIC DISEASE
    Mark Ginsberg; Fiscal Year: 2000
    ..The functions of these mutants, when transiently expressed in COS cells or in stable cell lines in Chinese hamster ovary cells, will be evaluated by; 1) conformation specific ..
  29. GENE DEFECTIVE IN HEREDITARY HEMOCHROMATOSIS
    William Sly; Fiscal Year: 2002
    ..the effects of the HH mutation(s) on the properties of the HLA-H gene product expressed in transfected COS cells. 2) Purify normal and mutant HLA-H proteins and identify their ligand(s) and other interacting proteins...
  30. PHOSPHORYLATION OF HSP 72 AND CARDIAC PROTECTION
    ANNE KNOWLTON; Fiscal Year: 2001
    ..In preliminary experiments with Cos cells, a common model for nuclear localization studies, it was determined that a change in phosphorylation at tyrosine ..
  31. Mechanisms of Growth Factor-Estrogen Receptor Crosstalk
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
  32. HUMAN TRP GENES IN RELATION TO CALCIUM ENTRY CHANNELS
    MICHAEL ZHU; Fiscal Year: 2001
    ..three Trps were cloned and two human Trps (Htrp1 and Htrp3) showed to facilitate Ca2+ entry when expressed in COS cells. Expression of antisense mouse trp sequences in murine L cells almost completely abolished the endogenous Ca2+ ..
  33. MOLECULAR SIGNALING IN VERTEBRATE EMBRYOGENESIS
    Yi Rao; Fiscal Year: 2001
    ..The central result in the current proposal, however, is the demonstration that supernatant from COS cells expressing His tagged Fringe induces mesoderm differentiation in animal caps...
  34. How does DHEA improve diabetic microvascular dysfunction
    JOSEPH DILLON; Fiscal Year: 2004
    ..Thus, the successful completion of our project will advance our long-term goal of developing novel therapeutic vasoactive agents, to decrease the morbidity related to diabetic vascular dysfunction. ..
  35. PHARMACOLOGIC BASIS OF SMOOTH MUSCLE HETEROGENEITY
    JULIUS ALLEN; Fiscal Year: 2001
    ..These data will define control of ion transport present in VSM and will help delineate the Na pump role in contractile regulation and will identify if the alpha 1T functions are specific for vascular smooth muscle. ..
  36. BIOLOGY OF THE CD2/E-RECEPTOR COMPLEX
    Malek Kamoun; Fiscal Year: 1992
    ..Many described roles of CD2 in T cell activation and differentiation make the study of CD2 structure-function extremely important for understanding and potentially manipulating the immune response...
  37. ACTH RELEASE BY DYN A--ROLE OF NMDA RECEPTORS
    Peter Cheng; Fiscal Year: 2000
    ..The mechanism of these actions are not known but they are all, at least in part, non-opioid. Thus, the studies may shed new light into the mechanisms behind the role of Dyn for treatment of cocaine and opioid abuse. ..
  38. THE TRAFFICKING OF MYELIN PROTEINS AND NEUROTROPHINS
    ERIC SHOOTER; Fiscal Year: 2003
    ..The experiments on the trafficking of epitope tagged or untagged PMP22 will be analyzed in Schwann or COS cells in culture, in myelinating co-cultures and in Trembler-J nerves...
  39. ACTION OF VITAMIN D AND ITS ANALOGS IN PROSTATE CANCER
    Coleman Gross; Fiscal Year: 2000
    ..It is hoped that the information gained will lead to more effective and less toxic treatments for prostate cancer. ..
  40. ROLES OF STAR IN REGULATED STEROIDOGENESIS
    Keith Parker; Fiscal Year: 2007
    ..We believe that the former model--based largely on transfection studies in non-steroidogenic COS cells--reflects aberrant effects of StAR over-expression in non-steroidogenic cells...
  41. MOLECULAR ANALYSIS OF TRANSFORMATION IN TEL/PDGFR MICE
    Michael Tomasson; Fiscal Year: 2003
    ..tyrosine phosphorylated and forms oligomers mediated by the TEL pointed (PNT) domain in vitro and in COS cells. In Preliminary Results the candidate describes the development of two animal models of hematopoietic malignancy ..
  42. AGGRECAN SUBSTRATE CONSTRUCTS FOR AGGRECANASE CATABOLISM
    Thomas Hering; Fiscal Year: 1999
    ..Cleavage products will be characterized by Western blot analysis using cleavage neoepitope-specific antisera. ..
  43. DISEASE VARIABLES IN HEREDITARY ANGIOEDEMA
    Bruce Zuraw; Fiscal Year: 2000
    ..These studies will contribute to our understanding of the molecular nature of HAE, and should begin to elucidate those factors that determine HAE patients' disease severity. ..
  44. Nutritional Modulation of Aging and Age-Related Diseases
    James Joseph; Fiscal Year: 2003
    ....
  45. RESPONSIVENESS OF GNRH RECEPTOR SIGNALING
    JIMMY NEILL; Fiscal Year: 2002
    ....
  46. AN ANIMAL MODEL OF GLUCOCORTICOID RESISTANCE
    Jonathan Scammell; Fiscal Year: 2007
    ..In summary, these studies will provide important insight into the physiological role of FKBP51 while at the same time adding substantial value to the Squirrel Monkey Resource at the University of South Alabama. ..
  47. MOLECULAR MECHANISM OF RENAL NA+ DEPENDENT COTRANSPORT
    Ana M Pajor; Fiscal Year: 2010
    ..Understanding how NaDC1 and SdcS work will help us to develop treatments for NaDC1-mediated diseases. ..
  48. In Vivo fluorescence correlation spectroscopy
    Yan Chen; Fiscal Year: 2003
    ..In addition, GFP tagged retinoid receptors will be used to perform an in vivo study to resolve their molecular oligomerization state in the presence and absence of ligands. ..
  49. THE EFFECT OF APOE ON OUTCOMES IN TBI ADULTS
    Yvette Conley; Fiscal Year: 2003
    ....
  50. VOLTAGE-GATED PROTON CHANNELS IN ALVEOLAR EPITHELIUM
    THOMAS DECOURSEY; Fiscal Year: 2003
    ..Demonstration that H+ current fluctuations result from stochastic gating of H+ channels will provide strong evidence that the molecule is an ion channel rather than a carrier. ..
  51. RPE65 IN RETINAL METABOLISM AND DEGENERATION
    Debra Thompson; Fiscal Year: 2005
    ..The long-term goals of this project are to elucidate the mechanisms by which RPE65 defects contribute to retinal degeneration, and to lay the groundwork for the development of therapeutic approaches to the disease. ..
  52. BETA 1 AND BETA 3 ADRENORECEPTORS
    JAMES GRANNEMAN; Fiscal Year: 2001
    ..Aim 2. To further characterize the differential signaling properties of beta1- beta3-AR and to examine the cellular and molecular basis. Specific Aim 3. To examine the molecular pharmacology of beta3-AR-selective agonists. ..
  53. REGULATION AND FUNCTION OF THE ALPHA SUBUNIT OF ENAC
    Christie Thomas; Fiscal Year: 2003
    ..The long-term objectives are to understand the regulation and function of ENaC in transepithelial Na+ transport. ..
  54. Analysis of Therapeutic Adipose Tissue Remodeling
    JAMES GRANNEMAN; Fiscal Year: 2004
    ..2) To examine adipose tissue cellular dynamics during therapeutic remodeling induced by agonist activation of beta3-adrenergic receptors and PPAR-gamma. ..
  55. Activation of inflammatory cells by flagellin
    STEVEN MIZEL; Fiscal Year: 2006
    ..Furthermore, the proposed studies should also provide insights into the factors that regulate TLR signaling and allow for complex patterns of host cell response. ..
  56. Regulation and function of cGMP in the nervous system
    David B Morton; Fiscal Year: 2011
    ..Viagra and other pharmacological treatments for erectile disfunction are probably just the tip of the iceberg for compounds that target cGMP mediated processes. ..
  57. The Genetic Basis of a Disease Free Model of Aging
    Yvette Conley; Fiscal Year: 2006
    ..This is the first study to propose to use lens transparency as a continuous, quantitative measure of the human aging process that will be suitable for genetic linkage and gene identification. ..
  58. Effects of intracellular angiotensin II
    Julia Cook; Fiscal Year: 2009
    ..abstract_text> ..
  59. OXIDATIVE SIGNALING THROUGH PROTEIN KINASE D
    Richard Waldron; Fiscal Year: 2003
    ..abstract_text> ..
  60. Oxidation Signaling via Protein Kinase D/Jun in GI Cells
    Richard Waldron; Fiscal Year: 2008
    ..Successful completion of these aims will improve our understanding of normal and pathological signaling mechanisms in the Gl tract, allowing us to pinpoint new targets for therapy. [unreadable] [unreadable] [unreadable]..
  61. Respiratory Immunity Against Agents of Bioterrorism
    STEVEN MIZEL; Fiscal Year: 2008
    ....
  62. Mitochondrial Genetics of Recovery After Brain Injury
    Yvette Conley; Fiscal Year: 2008
    ....
  63. Sgk1 in Na+ transport in fetal lung epithelia
    Christie Thomas; Fiscal Year: 2007
    ..abstract_text> ..
  64. VOLTAGE-GATED PROTON CHANNELS IN HUMAN NEUTROPHILS
    THOMAS DECOURSEY; Fiscal Year: 2008
    ..5) We will identify the proton channel molecule and gene. ..