fibroblasts

Summary

Summary: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.

Top Publications

  1. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
  2. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
  3. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
  4. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
  5. doi MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
    Thomas Thum
    Department of Medicine I, Interdisziplinares Zentrum fur Klinische Forschung IZKF, University of Wuerzburg, 97080 Wuerzburg, Germany
    Nature 456:980-4. 2008
  6. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
  7. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
  8. ncbi A DNA damage checkpoint response in telomere-initiated senescence
    Fabrizio d'Adda di Fagagna
    1 The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK 2 Present address IFOM FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy
    Nature 426:194-8. 2003
  9. pmc Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis
    Zhenbang Chen
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nature 436:725-30. 2005
  10. pmc Direct conversion of fibroblasts to functional neurons by defined factors
    Thomas Vierbuchen
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, Stanford University School of Medicine, 1050 Arastradero Road, Palo Alto, California 94304, USA
    Nature 463:1035-41. 2010

Detail Information

Publications359 found, 100 shown here

  1. ncbi Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 126:663-76. 2006
    ..Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions...
  2. ncbi Induction of pluripotent stem cells from adult human fibroblasts by defined factors
    Kazutoshi Takahashi
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Cell 131:861-72. 2007
    ..Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc...
  3. ncbi Multilineage potential of adult human mesenchymal stem cells
    M F Pittenger
    Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231 3043, USA
    Science 284:143-7. 1999
    ..These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential...
  4. ncbi Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..Recently, murine fibroblasts have been reprogrammed directly to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2,..
  5. doi MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
    Thomas Thum
    Department of Medicine I, Interdisziplinares Zentrum fur Klinische Forschung IZKF, University of Wuerzburg, 97080 Wuerzburg, Germany
    Nature 456:980-4. 2008
    ..show that microRNA-21 (miR-21, also known as Mirn21) regulates the ERK-MAP kinase signalling pathway in cardiac fibroblasts, which has impacts on global cardiac structure and function...
  6. ncbi Induced pluripotent stem cell lines derived from human somatic cells
    Junying Yu
    Genome Center of Wisconsin, Madison, WI 53706 1580, USA
    Science 318:1917-20. 2007
    ....
  7. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
    ..Such disease-specific stem cells offer an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development...
  8. ncbi A DNA damage checkpoint response in telomere-initiated senescence
    Fabrizio d'Adda di Fagagna
    1 The Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QR, UK 2 Present address IFOM FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy
    Nature 426:194-8. 2003
    ..Here we show that senescent human fibroblasts display molecular markers characteristic of cells bearing DNA double-strand breaks...
  9. pmc Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis
    Zhenbang Chen
    Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, Sloan Kettering Institute, 1275 York Avenue, New York, New York 10021, USA
    Nature 436:725-30. 2005
    ..Our results demonstrate the relevance of cellular senescence in restricting tumorigenesis in vivo and support a model for cooperative tumour suppression in which p53 is an essential failsafe protein of Pten-deficient tumours...
  10. pmc Direct conversion of fibroblasts to functional neurons by defined factors
    Thomas Vierbuchen
    Institute for Stem Cell Biology and Regenerative Medicine, Department of Pathology, Stanford University School of Medicine, 1050 Arastradero Road, Palo Alto, California 94304, USA
    Nature 463:1035-41. 2010
    ..Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four transcription factors...
  11. pmc Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells
    Ernesto Lujan
    Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:2527-32. 2012
    We recently showed that defined sets of transcription factors are sufficient to convert mouse and human fibroblasts directly into cells resembling functional neurons, referred to as "induced neuronal" (iN) cells...
  12. pmc Parkinson's disease patient-derived induced pluripotent stem cells free of viral reprogramming factors
    Frank Soldner
    The Whitehead Institute, Cambridge Center, MA 02142, USA
    Cell 136:964-77. 2009
    ..Here, we show that fibroblasts from five patients with idiopathic Parkinson's disease can be efficiently reprogrammed and subsequently ..
  13. ncbi Fibroblasts in cancer
    Raghu Kalluri
    Center for Matrix Biology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nat Rev Cancer 6:392-401. 2006
    ..implies that cells that are involved in angiogenesis and the response to injury, such as endothelial cells and fibroblasts, have a prominent role in the progression, growth and spread of cancers...
  14. pmc Induced pluripotent stem cells from a spinal muscular atrophy patient
    Allison D Ebert
    The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
    Nature 457:277-80. 2009
    ..Although patient fibroblasts have been used extensively to study spinal muscular atrophy, motor neurons have a unique anatomy and physiology ..
  15. doi Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
    John T Dimos
    Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Science 321:1218-21. 2008
    ..Although recent studies have demonstrated the reprogramming of human fibroblasts to a pluripotent state, it remains unclear whether these induced pluripotent stem (iPS) cells can be produced ..
  16. doi Copy number variation and selection during reprogramming to pluripotency
    Samer M Hussein
    Samuel Lunenfeld Research Institute, Toronto, Ontario M5T 3H7, Canada
    Nature 471:58-62. 2011
    ..significantly more CNVs are present in early-passage human iPS cells than intermediate passage human iPS cells, fibroblasts or human ES cells. Most CNVs are formed de novo and generate genetic mosaicism in early-passage human iPS cells...
  17. pmc Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts
    Akiko Doi
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Genet 41:1350-3. 2009
    ..of cytosine-phosphate-guanine (CpG) island shores in nine human iPS cell lines as compared to their parental fibroblasts. The differentially methylated regions (DMRs) in the reprogrammed cells (denoted R-DMRs) were significantly ..
  18. ncbi Extension of life-span by introduction of telomerase into normal human cells
    A G Bodnar
    Geron Corporation, Menlo Park, CA 94025, USA
    Science 279:349-52. 1998
    ..this hypothesis, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomerase catalytic subunit...
  19. pmc Senescence-associated secretory phenotypes reveal cell-nonautonomous functions of oncogenic RAS and the p53 tumor suppressor
    Jean Philippe Coppe
    Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
    PLoS Biol 6:2853-68. 2008
    ..Remarkably similar SASPs developed in normal fibroblasts, normal epithelial cells, and epithelial tumor cells after genotoxic stress in culture, and in epithelial tumor ..
  20. ncbi Mesenchymal stem cells within tumour stroma promote breast cancer metastasis
    Antoine E Karnoub
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 449:557-63. 2007
    ..Collectively, these data demonstrate that the tumour microenvironment facilitates metastatic spread by eliciting reversible changes in the phenotype of cancer cells...
  21. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..Here we demonstrate successful and efficient reprogramming of murine and human embryonic fibroblasts using doxycycline-inducible transcription factors delivered by PB transposition...
  22. doi Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution
    Nimet Maherali
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Harvard Stem Cell Institute, 185 Cambridge Street, Boston, MA 02114, USA
    Cell Stem Cell 1:55-70. 2007
    ..Here, using novel selection approaches, we have generated iPS cells from fibroblasts to characterize their epigenetic state...
  23. pmc Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
    Tarjei S Mikkelsen
    Broad Institute of Harvard and MIT, Cambridge, Massachusetts 02142, USA
    Nature 448:553-60. 2007
    ..generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised ..
  24. pmc Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs
    Gabsang Lee
    Developmental Biology Program, Sloan Kettering Institute, 1275 York Ave, USA
    Nature 461:402-6. 2009
    ..Our study illustrates the promise of iPSC technology for gaining new insights into human disease pathogenesis and treatment...
  25. pmc Parkin is recruited selectively to impaired mitochondria and promotes their autophagy
    Derek Narendra
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 183:795-803. 2008
    ..These results show that Parkin promotes autophagy of damaged mitochondria and implicate a failure to eliminate dysfunctional mitochondria in the pathogenesis of Parkinson's disease...
  26. pmc In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes
    Li Qian
    1Gladstone Institute of Cardiovascular Disease, San Francisco, California 94158, USA
    Nature 485:593-8. 2012
    ..We reported previously that cardiac fibroblasts,which represent 50%of the cells in the mammalian heart, can be directly reprogrammed to adult cardiomyocyte-..
  27. pmc Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion
    Francis Rodier
    Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Nat Cell Biol 11:973-9. 2009
    ..Thus, in addition to orchestrating cell-cycle checkpoints and DNA repair, a new and important role of the DDR is to allow damaged cells to communicate their compromised state to the surrounding tissue...
  28. pmc Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells
    Jose M Polo
    Howard Hughes Medical Institute and Department of Stem Cell and Regenerative Biology, Harvard University and Harvard Medical School, Cambridge, Massachusetts, USA
    Nat Biotechnol 28:848-55. 2010
    ..Here we show that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns...
  29. doi Generation of mouse induced pluripotent stem cells without viral vectors
    Keisuke Okita
    Center for iPS Cell Research and Application CiRA, Institute for Integrated Cell Material Sciences, Kyoto University, Kyoto 606 8507, Japan
    Science 322:949-53. 2008
    ..DNAs (cDNAs) of Oct3/4, Sox2, and Klf4 and the other containing the c-Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into ..
  30. pmc Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration
    Malea M Murphy
    Department of Human Genetics, University of Utah, 15 North 2030 East, Salt Lake City, UT 84112, USA
    Development 138:3625-37. 2011
    ..Connective tissue fibroblasts also are likely to play a role in regeneration, as connective tissue fibrosis is a hallmark of regenerating ..
  31. doi ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin
    Aaron A Goodarzi
    Genome Damage and Stability Centre, University of Sussex, East Sussex BN1 9RQ, UK
    Mol Cell 31:167-77. 2008
    ..These data suggest that the importance of ATM signaling for DSB repair increases as the heterochromatic component of a genome expands...
  32. ncbi Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN
    V Stambolic
    Amgen Institute, and Department of Medical Biophysics, University of Toronto, Ontario, Canada
    Cell 95:29-39. 1998
    ..In contrast, mPTEN-deficient immortalized mouse embryonic fibroblasts exhibit decreased sensitivity to cell death in response to a number of apoptotic stimuli, accompanied by ..
  33. ncbi Taking cell-matrix adhesions to the third dimension
    E Cukierman
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Science 294:1708-12. 2001
    ..These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms...
  34. ncbi BRAFE600-associated senescence-like cell cycle arrest of human naevi
    Chrysiis Michaloglou
    Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nature 436:720-4. 2005
    ..Thus, both in vitro and in vivo, BRAF(V600E)-expressing melanocytes display classical hallmarks of senescence, suggesting that oncogene-induced senescence represents a genuine protective physiological process...
  35. pmc Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors
    Masaki Ieda
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, San Francisco, CA 94158, USA
    Cell 142:375-86. 2010
    The reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) raises the possibility that a somatic cell could be reprogrammed to an alternative differentiated fate without first becoming a stem/progenitor cell...
  36. doi Patient-specific induced pluripotent stem-cell models for long-QT syndrome
    Alessandra Moretti
    Cardiology Division, First Department of Medicine, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany
    N Engl J Med 363:1397-409. 2010
    ..In long-QT syndrome type 1, mutations occur in the KCNQ1 gene, which encodes the repolarizing potassium channel mediating the delayed rectifier I(Ks) current...
  37. ncbi Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin
    Jacob Hanna
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Science 318:1920-3. 2007
    It has recently been demonstrated that mouse and human fibroblasts can be reprogrammed into an embryonic stem cell-like state by introducing combinations of four transcription factors...
  38. ncbi Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a
    M Serrano
    Cold Spring Harbor Laboratory, New York 11724, USA
    Cell 88:593-602. 1997
    ..Negation of ras-induced senescence may be relevant during multistep tumorigenesis...
  39. pmc Circulating fibrocytes traffic to the lungs in response to CXCL12 and mediate fibrosis
    Roderick J Phillips
    Division of Pulmonary and Critical Care Medicine, David Geffen School of Medicine at University of California, Los Angeles, USA
    J Clin Invest 114:438-46. 2004
    ..Pulmonary fibrosis was originally thought to be mediated solely by resident lung fibroblasts. Here we show that a population of human CD45(+)Col I(+)CXCR4(+) circulating fibrocytes migrates in response to ..
  40. ncbi In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
    Marius Wernig
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Nature 448:318-24. 2007
    ..Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors ..
  41. pmc Induced pluripotent stem cells generated without viral integration
    Matthias Stadtfeld
    Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA
    Science 322:945-9. 2008
    ..We generated mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc...
  42. pmc Common and unique mechanisms regulate fibrosis in various fibroproliferative diseases
    Thomas A Wynn
    Immunopathogenesis Section, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA
    J Clin Invest 117:524-9. 2007
    ....
  43. pmc A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells
    Maria C N Marchetto
    The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 143:527-39. 2010
    ..genetic model, we developed a culture system using induced pluripotent stem cells (iPSCs) from RTT patients' fibroblasts. RTT patients' iPSCs are able to undergo X-inactivation and generate functional neurons...
  44. ncbi Mesenchymal stem cells enhance wound healing through differentiation and angiogenesis
    Yaojiong Wu
    161 HMRC, University of Alberta, 113 Street and 87 Avenue, Edmonton, Alberta T6G 2E1, Canada
    Stem Cells 25:2648-59. 2007
    ..enhanced wound healing in normal and diabetic mice compared with that of allogeneic neonatal dermal fibroblasts or vehicle control medium...
  45. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    The morphology and cytoskeletal structure of fibroblasts, endothelial cells, and neutrophils are documented for cells cultured on surfaces with stiffness ranging from 2 to 55,000 Pa that have been laminated with fibronectin or collagen ..
  46. pmc The myofibroblast: one function, multiple origins
    Boris Hinz
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne EPFL, Bâtiment SG AA B143, Station 15, CH 1015 Lausanne, Switzerland
    Am J Pathol 170:1807-16. 2007
    ..Thus, whereas in lung fibrosis it seems that most myofibroblasts derive from resident fibroblasts, hepatic stellate cells are the main contributor for liver fibrosis and media smooth muscle cells are the main ..
  47. pmc Chromatin-modifying enzymes as modulators of reprogramming
    Tamer T Onder
    Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 483:598-602. 2012
    ....
  48. pmc Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease
    Marius Wernig
    The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 105:5856-61. 2008
    ..was recently shown that the four transcription factors Oct4, Sox2, Klf4, and c-Myc induce pluripotency in mouse fibroblasts. However, the therapeutic potential of induced pluripotent stem (iPS) cells for neural cell replacement ..
  49. pmc Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome
    Masayuki Yazawa
    Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305, USA
    Nature 471:230-4. 2011
    ..This study provides new opportunities for studying the molecular and cellular mechanisms of cardiac arrhythmias in humans, and provides a robust assay for developing new drugs to treat these diseases...
  50. pmc Epithelial-mesenchymal transition and its implications for fibrosis
    Raghu Kalluri
    Center for Matrix Biology, Beth Israel Deaconess Medical Center, 330 Brookline Ave DANA 514, Boston, Massachusetts 02215, USA
    J Clin Invest 112:1776-84. 2003
    ..and while EMT is well studied in the context of embryonic development, it also plays a role in the genesis of fibroblasts during organ fibrosis in adult tissues...
  51. pmc Arp2/3 is critical for lamellipodia and response to extracellular matrix cues but is dispensable for chemotaxis
    Congying Wu
    Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Cell 148:973-87. 2012
    ..Using fibroblasts derived from Ink4a/Arf-deficient mice, we generated a stable line depleted of Arp2/3 complex that lacks ..
  52. doi Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors
    Sayaka Sekiya
    Division of Organogenesis and Regeneration, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Nature 475:390-3. 2011
    ..transcription factors, comprising Hnf4α plus Foxa1, Foxa2 or Foxa3, that can convert mouse embryonic and adult fibroblasts into cells that closely resemble hepatocytes in vitro...
  53. pmc Reprogramming towards pluripotency requires AID-dependent DNA demethylation
    Nidhi Bhutani
    Baxter Laboratory for Stem Cell Biology, Institute for Stem Cell Biology and Regenerative Medicine, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305 5175, USA
    Nature 463:1042-7. 2010
    ..in reprogramming, we generated interspecies heterokaryons (fused mouse embryonic stem (ES) cells and human fibroblasts) that induce reprogramming synchronously, frequently and fast...
  54. ncbi Expression of a single transfected cDNA converts fibroblasts to myoblasts
    R L Davis
    Department of Genetics, Hutchinson Cancer Research Center, Seattle, Washington 98104
    Cell 51:987-1000. 1987
    5-azacytidine treatment of mouse C3H10T1/2 embryonic fibroblasts converts them to myoblasts at a frequency suggesting alteration of one or only a few closely linked regulatory loci...
  55. doi Direct conversion of human fibroblasts to multilineage blood progenitors
    Eva Szabo
    Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    Nature 468:521-6. 2010
    ..the ability to generate progenitors and mature cells of the haematopoietic fate directly from human dermal fibroblasts without establishing pluripotency...
  56. pmc Genome-scale DNA methylation maps of pluripotent and differentiated cells
    Alexander Meissner
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 454:766-70. 2008
    ..More generally, the results establish reduced representation bisulphite sequencing as a powerful technology for epigenetic profiling of cell populations relevant to developmental biology, cancer and regenerative medicine...
  57. doi Vitamin C enhances the generation of mouse and human induced pluripotent stem cells
    Miguel Angel Esteban
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, China
    Cell Stem Cell 6:71-9. 2010
    ..Our results therefore highlight a straightforward method for improving the speed and efficiency of iPSC generation and provide additional insights into the mechanistic basis of the reprogramming process...
  58. ncbi Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome
    Maria Eriksson
    Department of Human Genetics, New York State Institute for Basic Research in Developmental Disabilities, Staten Island, New York 10314, USA
    Nature 423:293-8. 2003
    ..Immunofluorescence of HGPS fibroblasts with antibodies directed against lamin A revealed that many cells show visible abnormalities of the nuclear ..
  59. doi Cancer-associated-fibroblasts and tumour cells: a diabolic liaison driving cancer progression
    Paolo Cirri
    Department of Biochemical Science, University of Florence, Florence, Italy
    Cancer Metastasis Rev 31:195-208. 2012
    ..circulating precursors, as macrophages and other inflammatory cells, endothelial cells and cancer-associated fibroblasts (CAFs). All these elements synergistically play a specific role in cancer progression...
  60. pmc MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes
    Tilanthi M Jayawardena
    Duke Cardiovascular Research Center, Duke University Medical Center, Durham, NC 27710, USA
    Circ Res 110:1465-73. 2012
    ..An ideal therapeutic approach would involve an effective method at achieving direct conversion of injured areas to functional tissue in situ...
  61. ncbi Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates
    N Q Balaban
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Nat Cell Biol 3:466-72. 2001
    ..The results put clear constraints on the possible molecular mechanisms for the mechanosensory response of focal adhesions to applied force...
  62. pmc Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome
    Xonia Carvajal-Vergara
    Department of Gene and Cell Medicine, Black Family Stem Cell Institute, Mount Sinai School of Medicine, New York, New York 10029, USA
    Nature 465:808-12. 2010
    ..These features correlate with a potential hypertrophic state. We also provide molecular insights into signalling pathways that may promote the disease phenotype...
  63. pmc Genetic comparison of mouse lung telocytes with mesenchymal stem cells and fibroblasts
    Yonghua Zheng
    Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China
    J Cell Mol Med 17:567-77. 2013
    ..study, the gene expression profile of murine lung TCs is compared with other differentiated interstitial cells (fibroblasts) and with stromal stem/progenitor cells...
  64. doi Direct conversion of fibroblasts into stably expandable neural stem cells
    Marc Thier
    Stem Cell Engineering Group at the Institute of Reconstructive Neurobiology, University of Bonn, Germany
    Cell Stem Cell 10:473-9. 2012
    ..Here we show direct derivation of stably expandable NSCs from mouse fibroblasts through a curtailed version of reprogramming to pluripotency...
  65. pmc Direct reprogramming of mouse and human fibroblasts into multipotent neural stem cells with a single factor
    Karen L Ring
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Cell Stem Cell 11:100-9. 2012
    ..Here we report the generation of induced neural stem cells (iNSCs) from mouse and human fibroblasts by direct reprogramming with a single factor, Sox2...
  66. pmc Stromal fibroblasts in cancer initiation and progression
    Neil A Bhowmick
    Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Nature 432:332-7. 2004
    ..by the tumour microenvironment, which includes extracellular matrix, blood vasculature, inflammatory cells and fibroblasts. Recent studies reveal that fibroblasts have a more profound influence on the development and progression of ..
  67. doi A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts
    Ronghui Li
    Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
    Cell Stem Cell 7:51-63. 2010
    Epithelial-to-mesenchymal transition (EMT) is a developmental process important for cell fate determination. Fibroblasts, a product of EMT, can be reset into induced pluripotent stem cells (iPSCs) via exogenous transcription factors but ..
  68. pmc Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism
    D Riveline
    Laboratory of Physical Spectrometry (CNRS, UMR 5588, Joseph Fourier University, French National Center for Scientific Research, BP87, , France
    J Cell Biol 153:1175-86. 2001
    ..Our experiments show that integrin-containing focal complexes behave as individual mechanosensors exhibiting directional assembly in response to local force...
  69. pmc miR-21 mediates fibrogenic activation of pulmonary fibroblasts and lung fibrosis
    Gang Liu
    Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Exp Med 207:1589-97. 2010
    Uncontrolled extracellular matrix production by fibroblasts in response to tissue injury contributes to fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF), a progressive and ultimately fatal process that currently has no cure...
  70. ncbi Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells
    Cedric Gaggioli
    Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Nat Cell Biol 9:1392-400. 2007
    Imaging of collectively invading cocultures of carcinoma cells and stromal fibroblasts reveals that the leading cell is always a fibroblast and that carcinoma cells move within tracks in the extracellular matrix behind the fibroblast...
  71. ncbi Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence
    Masashi Narita
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 113:703-16. 2003
    ..In this study, we describe a distinct heterochromatic structure that accumulates in senescent human fibroblasts, which we designated senescence-associated heterochromatic foci (SAHF)...
  72. doi Hallmarks of cancer: interactions with the tumor stroma
    Kristian Pietras
    Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles vag 2, SE 171 77 Stockholm
    Exp Cell Res 316:1324-31. 2010
    ..tumor cells do not act in isolation, but rather subsist in a rich microenvironment provided by resident fibroblasts, endothelial cells, pericytes, leukocytes, and extra-cellular matrix...
  73. ncbi Formation and function of the myofibroblast during tissue repair
    Boris Hinz
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
    J Invest Dermatol 127:526-37. 2007
    ..Moreover, intervening with myofibroblast stress perception and transmission offers novel strategies to reduce tissue contracture; stress release leads to the instant loss of contraction and promotes apoptosis...
  74. pmc Cellular and molecular mechanisms of fibrosis
    T A Wynn
    Immunopathogenesis Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Pathol 214:199-210. 2008
    ..e. TLRs) on fibroblasts. Cytokines (IL-13, IL-21, TGF-beta1), chemokines (MCP-1, MIP-1beta), angiogenic factors (VEGF), growth factors (..
  75. pmc Distinct epigenomic landscapes of pluripotent and lineage-committed human cells
    R David Hawkins
    Ludwig Institute for Cancer Research, La Jolla, CA 92093, USA
    Cell Stem Cell 6:479-91. 2010
    ..By comparing the chromatin-modification profiles and DNA methylomes in hESCs and primary fibroblasts, we find that nearly one-third of the genome differs in chromatin structure...
  76. ncbi Inflammation in wound repair: molecular and cellular mechanisms
    Sabine A Eming
    Department of Dermatology, University of Cologne, Cologne, Germany
    J Invest Dermatol 127:514-25. 2007
    ..In this paper, we review cellular and molecular mechanisms controlling inflammation in cutaneous tissue repair and provide a rationale for targeting the inflammatory phase in order to modulate the outcome of the healing response...
  77. pmc Oxidative stress in cancer associated fibroblasts drives tumor-stroma co-evolution: A new paradigm for understanding tumor metabolism, the field effect and genomic instability in cancer cells
    Ubaldo E Martinez-Outschoorn
    Department of Medical Oncology, Thomas Jefferson University, Philadelphia, PA, USA
    Cell Cycle 9:3256-76. 2010
    ..In this model, cancer cells induce oxidative stress in cancer-associated fibroblasts, which then acts as a "metabolic" and "mutagenic" motor to drive tumor-stroma co-evolution, DNA damage and ..
  78. pmc Essential function of TORC2 in PKC and Akt turn motif phosphorylation, maturation and signalling
    Tsuneo Ikenoue
    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA
    EMBO J 27:1919-31. 2008
    ..Our study demonstrates that mTORC2 is involved in post-translational processing of PKC by facilitating TM and HM phosphorylation and reveals a novel function of mTORC2 in cellular regulation...
  79. pmc Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-beta1: potential role in idiopathic pulmonary fibrosis
    Brigham C Willis
    Department of Anesthesiology Critical Care Medicine, Childrens Hospital Los Angeles, California, USA
    Am J Pathol 166:1321-32. 2005
    ..These results suggest that AECs undergo EMT when chronically exposed to TGF-beta1, raising the possibility that epithelial cells may serve as a novel source of myofibroblasts in IPF...
  80. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..Transposons carrying 2A peptide-linked reprogramming factors induced reprogramming of mouse embryonic fibroblasts with equivalent efficiencies to retroviral transduction...
  81. ncbi Myofibroblasts and mechano-regulation of connective tissue remodelling
    James J Tomasek
    Department of Cell Biology, University of Oklahoma Health Sciences Center, P O Box 26901, Oklahoma City, OK 73190, USA
    Nat Rev Mol Cell Biol 3:349-63. 2002
    ....
  82. pmc Cancer-associated stromal fibroblasts promote pancreatic tumor progression
    Rosa F Hwang
    Department of Surgical Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77230 1402, USA
    Cancer Res 68:918-26. 2008
    ..These data indicate that stellate cells have an important role in supporting and promoting pancreatic cancer. Identification of HPSC-derived factors may lead to novel stroma-targeted therapies for pancreatic cancer...
  83. doi iPS cells produce viable mice through tetraploid complementation
    Xiao yang Zhao
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    Nature 461:86-90. 2009
    ..of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell ..
  84. pmc Induction of human neuronal cells by defined transcription factors
    Zhiping P Pang
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, 265 Campus Drive, Stanford, California 94305, USA
    Nature 476:220-3. 2011
    ..of three transcription factors, Brn2 (also known as Pou3f2), Ascl1 and Myt1l, can efficiently convert mouse fibroblasts into functional induced neuronal (iN) cells...
  85. pmc Exploring long-term protection of normal human fibroblasts and epithelial cells from chemotherapy in cell culture
    Pasha Apontes
    Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    Oncotarget 2:222-33. 2011
    ....
  86. doi Multilineage differentiation potential of human dermal skin-derived fibroblasts
    Katrin Lorenz
    Department of Cell Techniques and Applied Stem Cell Biology, Biotechnological Biomedical Center, University of Leipzig, Leipzig, Germany
    Exp Dermatol 17:925-32. 2008
    Dermal skin-derived fibroblasts from rodent and human have been found to exhibit mesenchymal surface antigen immunophenotype and differentiation potential along the three main mesenchymal-derived tissues: bone, cartilage and fat...
  87. ncbi Friends or foes - bipolar effects of the tumour stroma in cancer
    Margareta M Mueller
    Group Tumor and Microenvironment, German Cancer Research Centre, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nat Rev Cancer 4:839-49. 2004
    ..What have we recently learned from studying tumour-stroma interactions, and will this help to define new targets for therapy?..
  88. doi Wnt activity defines colon cancer stem cells and is regulated by the microenvironment
    Louis Vermeulen
    Laboratory for Experimental Oncology and Radiobiology LEXOR, Center for Experimental Molecular Medicine CEMM, Academic Medical Center AMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands
    Nat Cell Biol 12:468-76. 2010
    ..We therefore propose that stemness of colon cancer cells is in part orchestrated by the microenvironment and is a much more dynamic quality than previously expected that can be defined by high Wnt activity...
  89. pmc PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy
    Noriyuki Matsuda
    Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8506, Japan
    J Cell Biol 189:211-21. 2010
    ..Some pathogenic mutations of PINK1 and Parkin interfere with the aforementioned events, suggesting an etiological importance. These results provide crucial insight into the pathogenic mechanisms of PD...
  90. pmc MicroRNA-mediated conversion of human fibroblasts to neurons
    Andrew S Yoo
    Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University, Stanford, California 94305, USA
    Nature 476:228-31. 2011
    ..Here we show that expression of miR-9/9* and miR-124 (miR-9/9*-124) in human fibroblasts induces their conversion into neurons, a process facilitated by NEUROD2...
  91. doi Direct generation of functional dopaminergic neurons from mouse and human fibroblasts
    Massimiliano Caiazzo
    Stem Cells and Neurogenesis Unit, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy
    Nature 476:224-7. 2011
    ..as Nr4a2) and Lmx1a--that are able to generate directly functional dopaminergic neurons from mouse and human fibroblasts without reverting to a progenitor cell stage...
  92. pmc Caspases 3 and 7: key mediators of mitochondrial events of apoptosis
    Saquib A Lakhani
    Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Science 311:847-51. 2006
    ..b>Fibroblasts lacking both enzymes were highly resistant to both mitochondrial and death receptor-mediated apoptosis, ..
  93. ncbi Gain of function of a p53 hot spot mutation in a mouse model of Li-Fraumeni syndrome
    Gene A Lang
    Department of Molecular Genetics, Section of Cancer Genetics, The University of Texas MD Anderson Cancer Center and The University of Texas Graduate School of Biomedical Sciences, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 119:861-72. 2004
    ..Correspondingly, the embryonic fibroblasts from the p53515A/515A mutant mice displayed enhanced cell proliferation, DNA synthesis, and transformation ..
  94. pmc Reinitiation involving upstream ORFs regulates ATF4 mRNA translation in mammalian cells
    Krishna M Vattem
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proc Natl Acad Sci U S A 101:11269-74. 2004
    ..These results suggest that the mechanism of translation reinitiation involving uORFs is conserved from yeast to mammals...
  95. ncbi hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase
    H Vaziri
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Cell 107:149-59. 2001
    ..In contrast, expression of a catalytically inactive hSir2 protein potentiates p53-dependent apoptosis and radiosensitivity. We propose that hSir2 is involved in the regulation of p53 function via deacetylation...
  96. ncbi FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly
    Donna J Webb
    Department of Cell Biology, UVA School of Medicine, P O Box 800732, Charlottesville, Virginia 22908 0732, USA
    Nat Cell Biol 6:154-61. 2004
    ....
  97. pmc Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells
    Deepa Subramanyam
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 29:443-8. 2011
    ..cell cycle-regulating (ESCC) family of microRNAs (miRNAs) enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells...
  98. pmc Direct reprogramming of adult human fibroblasts to functional neurons under defined conditions
    Rajesh Ambasudhan
    Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell Stem Cell 9:113-8. 2011
    ..MYT1L and BRN2) is sufficient to directly reprogram postnatal and adult human primary dermal fibroblasts (mesoderm) to functional neurons (ectoderm) under precisely defined conditions...
  99. pmc Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis
    Beatrix Bartok
    Division of Rheumatology, Allergy, and Immunology, UCSD School of Medicine, La Jolla, CA 92093, USA
    Immunol Rev 233:233-55. 2010
    ..New agents that target FLS could potentially complement the current therapies without major deleterious effect on adaptive immune responses...
  100. ncbi DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
    Christopher J Bakkenist
    Department of Hematology Oncology, St Jude Children s Research Hospital, 332 North Lauderdale Street, Memphis, Tennessee 38105, USA
    Nature 421:499-506. 2003
    ....
  101. doi Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors
    Pengyu Huang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China
    Nature 475:386-9. 2011
    ..Here we demonstrate the direct induction of functional hepatocyte-like (iHep) cells from mouse tail-tip fibroblasts by transduction of Gata4, Hnf1α and Foxa3, and inactivation of p19(Arf)...

Research Grants75

  1. Mice Humanized with Hepatocytes and iPS Cells from Patients with Metabolic Diseas
    Stephen C Strom; Fiscal Year: 2010
    ..Challenge Topic 14-DK-101, "Induced Pluripotent stem cells-cellular and humanized mouse" Somatic cells such as fibroblasts from patients with diseases can be used to create cell lines, tissues, and perhaps, organ systems, through ..
  2. Optimization of DNA Repair by Abh8
    Lauren Endres; Fiscal Year: 2013
    ..e., Pifithrin alpha);ATM-/- and p53-/- MEFs;human AT fibroblasts and Li Fraumeni lymphoblastoid cells...
  3. Anti-Inflammatory Glycosaminoglycan Ethers for Treatment of Periodontitis
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2011
    ..and inhibition of matrix metalloproteinase (MMP) production by interleukin-12 (IL-12)-stimulated gingival fibroblasts. GlycoMira is developing semi-synthetic glycosaminoglycan ethers (SAGEs) as safe and effective inflammation-..
  4. Physiological role of PRDM16 in brown fat development and energy balance
    Patrick Seale; Fiscal Year: 2011
    ..Transplantation of PRDM16 expressing fibroblasts. The goal is to develop an autologous transplantation protocol using PRDM16-expressing precursor cells to form ..
  5. Development of a Nonpsychotropic Cannabinoid Agonist for the Treatment of Sclerod
    Robert B Zurier; Fiscal Year: 2012
    ..Current therapy is limited, with 10 year survival of 55%. The mechanisms leading to activation of fibroblasts and the subsequent over production of ECM are not clear...
  6. Role of Txnip in adapting fuel metabolism to nutritional state
    SIMON HUI; Fiscal Year: 2013
    ..To show the causative link between NADPH levels and mitochondrial function, NADPH levels in TKO embryonic fibroblasts will be modulated by adenoviral expression of NAD kinase...
  7. Pathogenesis and Treatment of Liver Disease in Transaldolase Deficiency
    Andras Perl; Fiscal Year: 2013
    ..In fibroblasts and lymphoblasts of the first TAL-deficient patient, we found that deletion of S171 caused mis- folding, ..
  8. Regulation of mural cells during pulmonary capillary formation
    Stephen E McGowan; Fiscal Year: 2013
    ..expansion of the alveolar capillary network requires cooperation among endothelial cells, pericytes, and lung fibroblasts (LF)...
  9. Lung Lysyl Oxidase Regulation by Metal Ion Homeostasis
    Wande Li; Fiscal Year: 2013
    ..Previous studies by this lab investigating the phenotype change from Cd sensitive to Cd resistant of rat lung fibroblasts (RFL6) illustrated downregulation of LO by Cd at mRNA, protein and catalytic levels...
  10. Patient-Derived Stem Cells for Phosphoproteomic Profiling Neuropsychopathology
    Dieter A Wolf; Fiscal Year: 2011
    ..Recent advances have allowed the conversion of patient-specific human fibroblasts to human induced pluripotent stem cells (hIPSCs), cells which become sufficiently dedifferentiated to a ..
  11. The Kallikrein/Kinin-Mast Cell Interaction in Volume Overload
    CHIH CHANG KEVIN WEI; Fiscal Year: 2013
    ..Aim 2 Determine whether cardiomyocytes or fibroblasts are the source of increased kallikrein expression and BK release in response to stretch in vitro and VO in vivo...
  12. TARGETING THE MARCKS-FAMILY PROTEINS TO INHIBIT NEUTROPHIL MIGRATION
    Mary K Sheats; Fiscal Year: 2013
    ..actin structure formation in the neuron-like cell line PC12 and membrane ruffling and lamellae formation in Ltk fibroblasts. This proposal will investigate the hypothesis that both MARCKS and MARCKSL1 have essential and non-redundant ..
  13. Parathyroid Hormone & Vitamin D Effects on Salt Sensitivity and Vascular Function
    ANNA LIZA BELEN VALINA TOTH; Fiscal Year: 2010
    ..deleterious physiological effects within the vasculature and myocardium such as vasoconstriction, activation of fibroblasts, insulin resistance, dyslipidemia, fat deposition and down regulation of endothelial nitric oxide - all of ..
  14. Metabolic mechanisms of antiestrogen resistance in breast cancer
    UBALDO EMILIO MARTINEZ OUTSCHOORN; Fiscal Year: 2013
    ..These cell lines that I generate will be cultured with either fibroblasts or ER+ carcinoma cells...
  15. Alzheimer's Disease Therapeutic
    Trevor P Castor; Fiscal Year: 2012
    ..activator, dramatically enhance the generation of non-amyloidogenic soluble amyloid precursor protein (sAPP) in fibroblasts from AD patients...
  16. Anti-Inflammatory Glycosaminoglycan Ethers for Treatment of Periodontitis
    JUSTIN RODNEY SAVAGE; Fiscal Year: 2013
    ..E2, and matrix metalloproteinases (MMP) 1, 2, 3, and 9 release from macrophages and human gingival fibroblasts. Second, it blocks CR3-mediated internalization of P. gingivalis by macrophages. Third, it inhibits P...
  17. CPG METHYLATION AND MUTATION
    Gerd P Pfeifer; Fiscal Year: 2013
    ..Using cell culture models, we will expose non-transformed human cells (fibroblasts, bronchial epithelial cells and mammary epithelial cells) to several chemical carcinogens...
  18. Mechanisms of Stem Cell Self-Renewal and Aging
    PAUL CHRISTIAN SCHILLER; Fiscal Year: 2013
    ..Progerin is elevated in skin fibroblasts of healthy older individuals due to sporadic use of the cryptic splice site in exon 11...
  19. The Somatotropic Axis and Health Aging: A Search for Mechanisms
    Andrzej Bartke; Fiscal Year: 2013
    ..Project 3 will study mechanisms of stress resistance in fibroblasts and pre-adipocytes and evaluate longevity and age-sensitive traits in novel mutant mice developed in Project 2...
  20. Epigenetic Analysis of iPS Cell Models of Aging and Alzheimer's Disease
    Bruce A Yankner; Fiscal Year: 2010
    ..by exploring the physiology and epigenetics of iPS cells and iPS cell-derived neurons generated from skin fibroblasts of individuals at different ages and with AD...
  21. A novel telomerase expressing lung fibroblast phenotype
    Sem H Phan; Fiscal Year: 2012
    ..central hypothesis of this project is that induction of telomerase expression in lung injury and fibrosis in fibroblasts promotes their survival with increased resistance to apoptosis and thus promotes fibrosis...
  22. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..We will also employ mouse embryo fibroblasts and primary culture cells derived from mouse knock-out models...
  23. Development of a Porcine Model of Atherosclerosis
    Christopher Rogers; Fiscal Year: 2010
    ..This proposal specifically outlines the development of porcine fibroblasts with a disrupted LDLR gene. A gene targeting vector will be constructed to disrupt the coding region of LDLR...
  24. Regulation of Genomic and Epigenomic Stability at CpG Sites
    Alfonso Bellacosa; Fiscal Year: 2013
    ..assess which TDG function is required for ES cell differentiation in vitro and for phenotypes in mouse embryo fibroblasts. We will also study by ChIP-seq the relationship between promoter occupancy by TDG and methylation patterns on ..
  25. IGFBP-5 and Hsp70 in Extracellular Matrix Homeostasis and Fibrosis
    Kristen L Veraldi; Fiscal Year: 2013
    ..has demonstrated increased expression of insulin-like growth factor binding protein 5 (IGFBP-5) in scleroderma fibroblasts and fibrotic lung tissue from patients with idiopathic pulmonary fibrosis...
  26. The Mucolipin TRP Ion Channels
    Haoxing Xu; Fiscal Year: 2013
    ..methods, we will determine whether iron release from endo-lysosomes is impaired in TRPML1-deficient skin fibroblasts. Our second aim is to investigate the roles of TRPML1 in the lysosome-mediated cell biological functions that ..
  27. Hematopoietic Stem Cell Transplantation in Osteogenesis Imperfecta
    Meenal Mehrotra; Fiscal Year: 2012
    ..few other cells like mast cells and osteoclasts, while MSCs generate a number of mesenchymal cells including fibroblasts, adipocytes, chondrocytes and osteoblasts...
  28. Adiponectin and fetal programming
    Jianhua Shao; Fiscal Year: 2013
    ..Furthermore, mouse embryonic fibroblasts (MEFs) from obese dams exhibited remarkable high efficiency in adipocyte differentiation, while protein levels ..
  29. Regulation of vasculature by estrogen receptors during aging in mouse models
    Margaret Markiewicz; Fiscal Year: 2013
    ..aim 3, we will determine the mechanism of ER?? and ER?? on the interaction between endothelial cells and fibroblasts in vitro, using 3D co-culture system...
  30. Maternal Obesity affects AMP-Kinase in Muscle Cell Differentiation
    Min Du; Fiscal Year: 2013
    ..Myocytes, adipocytes and fibroblasts in fetal SM are derived from mesenchymal stem cells (MSC)...
  31. Deriving hepatocytes from disease specific iPS to treat metabolic liver disorders
    Namita Roy-Chowdhury; Fiscal Year: 2013
    ..The specific aim 1 is (a) Generating and characterizing iPS cells from skin fibroblasts from normal subjects and CN1 patients, (b) correcting the genetic defect by homologous recombination at a ..
  32. TNF Family Members and Control of Fibrotic Disease
    Michael Croft; Fiscal Year: 2013
    ..Collagen deposition is thought mediated by deregulation of epithelial cells, macrophages, and fibroblasts, and inflammatory cells such as Th2 cells and eosinophils may play roles in promoting the activity of these ..
  33. Development of a Porcine Model of Atherosclerosis
    Christopher Rogers; Fiscal Year: 2012
    ..LDLR fetal fibroblasts that we developed in Phase I will be used as nuclear donors for somatic cell nuclear transfer...
  34. ATM Controls Genomic Stability in Pluripotent Stem Cells
    JASON MARK BECKTA; Fiscal Year: 2013
    ..As we are in the process of developing induced pluripotent cells from normal and disease-specific fibroblasts, preliminary studies were conducted in human embryonic stem cells...
  35. Genetic Analysis of FAK Activity
    David D Schlaepfer; Fiscal Year: 2012
    ..Moreover, as FAK-null mouse embryo fibroblasts (MEFs) exhibit both proliferation and motility defects, it remains undetermined whether FAK activity is ..
  36. Dietary Cholesterol and Defects in Cholesterol Synthesis
    ROBERT DAVID STEINER; Fiscal Year: 2013
    ..These latter studies will be conducted in vitro using SLOS and control skin fibroblasts, SLOS mouse brain-derived cells, and SLOS human brain tissues...
  37. Inactivation of Hdac3 in the cause, prevention, and treatment of HCC
    Scott W Hiebert; Fiscal Year: 2013
    ..Moreover, Hdac3 deletion was even lethal to murine embryonic fibroblasts in vitro...
  38. Epigenetic Alterations in IPF Fibroblastic Foci
    YAN SANDERS; Fiscal Year: 2010
    ..Our lab has established that Thy-1, which suppresses the fibrotic phenotype of fibroblasts, is absent in fibroblastic foci of IPF, that its absence can be caused by methylation at the Thy-1 promoter ..
  39. Human cytomegalovirus entry into epithelial and endothelial cells
    David C Johnson; Fiscal Year: 2013
    ..related to a capacity to infect a broad array of cell types including epithelial, endothelial and glial cells, fibroblasts, monocyte-macrophages (M/M) and neurons...
  40. Germ Cell Migration in Drosophila.
    Ruth Lehmann; Fiscal Year: 2012
    ..In adults, migratory cell types include the cells of the immune system, fibroblasts during wound healing, and cancer cells during metastasis...
  41. Development of a Porcine Model of Ataxia-Telangiectasia
    Christopher Rogers; Fiscal Year: 2011
    ..This proposal specifically outlines the development of porcine fibroblasts with mutated ATM alleles...
  42. Caveolins in the Pathogenesis of Idiopathic Pulmonary Fibrosis
    Dan N Predescu; Fiscal Year: 2010
    ..distribution of TGF?-R and cav-2 in healthy lung tissue versus lungs with IPF, and I will isolate human lung fibroblasts to create a model (fibroblasts with low levels of cav-2) that will allow separating the contribution of cav-2, ..
  43. Regulation of Fibronectin Matrix Assembly by uPAR
    PAULA J MC KEOWN-LONGO; Fiscal Year: 2012
    ..During the next funding period, we intend to dissect the molecular mechanisms which control this pathway in fibroblasts, osteosarcoma cells and MDA-MB-231 breast cancer cells...
  44. Regulation of Lung Fibrosis by Alternatively Activated Macrophages and Arginase-1
    ROBERT MATTHEW TIGHE; Fiscal Year: 2013
    ..Arginase-1 is produced both by alternatively activated macrophages and fibroblasts in response to non-infectious lung injury. It has the potential to directly mediate fibrogenesis...
  45. Therapeutic use of small-molecule HGF-mimetic for emphysema
    BERT J W M OEHLEN; Fiscal Year: 2010
    ..HGF production in pulmonary fibroblasts of emphysema patients is known to be impaired...
  46. Investigating the pathogenesis of CoQ10 deficiencies
    Catarina M Quinzii; Fiscal Year: 2013
    ..collected biological samples from 84 patients (71 families) with documented CoQ10 deficiency in muscle and/or fibroblasts, or suspected CoQ10 deficiency based on the clinical manifestations as well as the response to CoQ10 ..
  47. Thrombospondin 4 A1
    JOHN W LAWLER; Fiscal Year: 2010
    ..We have found that COMP binds to aggrecan, as well as to integrins in chondrocytes and fibroblasts. In addition, growth plate disorganization is observed in TSP-1-, TSP-3-, and COMP-null mice...
  48. Mouse Models of Non-Syndromic Cardiac Progeria
    Ali J Marian; Fiscal Year: 2012
    ..and also will enable analyzing involvement of multiple cardiac and non-cardiac cell types, including myocytes, fibroblasts and conduction system...
  49. TRANSFORMING GROWTH FACTOR - BETA MEDIATES EFFECTS OF HYPOXIA IN NEWBORN LUNG
    Namasivayam Ambalavanan; Fiscal Year: 2012
    ..TGF-( activation is inhibited by fibroblasts in the lung which express the cell surface glycoprotein Thy-1...
  50. In vitro Type Two Diabetes Mellitus Tissue Model to Investigate Insulin Resistanc
    KELLY ANNE BURKE; Fiscal Year: 2013
    ..Aim 2 will add complexity to the model by incorporating human fibroblasts and monocytes, which may activate to M1 macrophages to create a pro-inflammatory environment like that found in ..
  51. Functional Studies of Variants of the Bipolar Risk Gene CACNA1C in Human Neurons
    JEN PAN; Fiscal Year: 2013
    ..Recently, mouse and human fibroblasts have been directly converted to functional neuronal cells through ectopic expression of three transcription ..
  52. STRUCTURE AND FUNCTION OF HEPARIN COFACTOR II
    Douglas M Tollefsen; Fiscal Year: 2010
    ..in plasma that can be activated by dermatan sulfate (DS) proteoglycans on vascular smooth muscle cells and fibroblasts. In a murine model of HCII deficiency, we showed that HCII modulates the response to arterial injury...
  53. Effect of Maspin on Corneal Heme-and lymph- angiogenesis
    Sally S Twining; Fiscal Year: 2013
    ..one of which is maspin, a non-inhibitory Serpin that regulates adhesion, migration and invasion of corneal fibroblasts as well as other cell types, including carcinoma cells...
  54. Role of AIB1 in malignant progression
    Anna Tate Riegel; Fiscal Year: 2013
    ..We will establish the conditions in which endothelial cells co cultured with epithelium, fibroblasts and monocytes, are influenced by changes in AIB1 expression...
  55. Studying a Human Stem Cell Disease Using iPS Technology
    Steven E Artandi; Fiscal Year: 2010
    ..We plan to reprogram fibroblasts from DC patients to iPS cells, which will facilitate analysis of the DC defect in a self-renewing population of ..
  56. 3D Skin Model to Test Toxic and Sensitizing Potentials of Environmental Chemicals
    Akira Takashima; Fiscal Year: 2010
    ..Our objective is to develop a 3D multi-channel skin biosensor system that is composed of KCs, DCs, and fibroblasts (FBs) and that allows simultaneous detection of skin irritants (measured by ATP release) and skin sensitizers (..
  57. Mechanisms of cancer initiation by TRIM32
    Molly F Kulesz-Martin; Fiscal Year: 2012
    ..Piasy is a pro- apoptotic factor, inducing apoptosis in CML cells, human fibroblasts and mouse keratinocytes...
  58. CTRIP: Molecular phenotypes of rapidly progressive idiopathic pulmonary fibrosis
    Galen B Toews; Fiscal Year: 2010
    ..Furthermore, IPF transbronchial biopsies (TBB)-derived fibroblasts exhibit similar migratory and proliferative properties compared with SLB-derived fibroblasts from the same ..
  59. Negative regulation of NF-kB and inflammation by TAX1BP1
    Edward W Harhaj; Fiscal Year: 2012
    ..Although homozygous null mice are not viable, murine embryonic fibroblasts (MEFs) from these mice exhibit elevated and persistent NF-kB as well as c-jun N-terminal kinase (JNK) ..
  60. Aging and Apoptosis
    Brian A Herman; Fiscal Year: 2012
    ..aims: 1) Determine the age-dependent sensitivity of hepatocytes, cortical neurons, mesenchepalic cells and fibroblasts obtained from caspase-2^, caspase-2 liver specific (caspase-2L'~\ caspase-2 brain specific (caspase-28&..
  61. Control of liver autophagy by phosphatidic acid signaling
    GILBERT DIPAOLO; Fiscal Year: 2013
    ..showing that lipid droplets are more prominent in Pld1 knockout liver from starved mice as well as in knockout fibroblasts, our results strongly suggest that PLD1 is implicated in starvation- induced macrolipophagy...
  62. Lung Toxicity of Carbon Nanotubes in Models of Pre-Existing Respiratory Disease
    JAMES CHRISTOPHER BONNER; Fiscal Year: 2010
    ..In Aim 3, we will evaluate cell signaling pathways activated by CNTs in cultured macrophages, fibroblasts, and mesothelial cells that lead to the production of pro-fibrogenic cytokines and growth factors that play ..
  63. Macrolide Accumulation by Host Cells in the Gingiva
    John D Walters; Fiscal Year: 2010
    ..Preliminary studies suggest that active transport plays an important role in this process. Cultured gingival fibroblasts, epithelial cells and PMN-like HL-60 cells appear to take up clarithromycin by a concentrative and saturable ..
  64. Directed Self Assembly of Complex Branching Micro-Tissues
    JEFFREY R contact MORGAN; Fiscal Year: 2010
    ..define the rules of directed self assembly, we propose to use five cell lines and their mixtures, normal human fibroblasts (NHF), a hepatocyte cell line (H35), smooth muscle cells (SMC) and two endothelial cell lines;human umbilical ..
  65. Selenium, Glutathione Peroxidase-1, and Homocysteine-induced Cardiac Remodeling
    Jacob Joseph; Fiscal Year: 2009
    ..Our preliminary data strongly suggest that Hhe acts via increased oxidant stress in cardiac fibroblasts to cause myocardial fibrosis and diastolic dysfunction...
  66. Role of Tumor and Stromal Cell Metabolism in Stress Adaptation and Progression
    EILEEN contact WHITE; Fiscal Year: 2010
    ..Growth to high density induces fibroblasts to alter metabolism, activate autophagy, exit the cell cycle, and enter quiescence...
  67. ACTIVATION OF PERIODONTAL FIBROBLASTS VIA CD40
    Timothy Blieden; Fiscal Year: 2000
    ..In most areas of infiltration, fibroblasts are non-existent...
  68. CELLULAR & MOLECULAR BASIS FOR BASAL CELL NEVUS SYNDROME
    HONNAVARA ANANTHASWAMY; Fiscal Year: 1992
    ..Preliminary studies have indicated that skin fibroblasts from BCNS individuals are hypersensitive to killing by UV-B/A (280-400 nm), but not UV-C (254 nm) radiation, ..
  69. Studies of Collagen Gene Regulation in Two Murine Models
    Stephen Clark; Fiscal Year: 2005
    ..proposed for the identification of genes involved in the regulation of extracellular matrix synthesis in dermal fibroblasts. Two experimental strategies are planned and are encompassed in three specific aims...
  70. BREAST TUMOR PROXIMAL STROMA INTERACTION AND AROMATASE
    Serdar Bulun; Fiscal Year: 2000
    ..Media conditioned by breast cancer cells stimulated aromatase activity of glucocorticoid- treated adipose fibroblasts, which was potentiated by E2 pretreatment of the cancer cells...
  71. Bone marrow-derived fibroblasts in skin wound healing
    Omaida C Velazquez; Fiscal Year: 2010
    Bone marrow-derived fibroblasts in skin wound healing. Nearly 25% of all diabetic patients, with foot ulcers, will inevitably progress to major limb amputation...
  72. Platelet-Derived Growth Factor, Remodeling and Asthma
    Monica Kraft; Fiscal Year: 2010
    ..Platelet-derived growth factor (PDGF) is a mediator produced by fibroblasts that is induced by IL-13 and is instrumental in tissue repair...
  73. REGULATION OF TISSUE FACTOR PATHWAY INHIBITOR EXPRESSION
    MADHU BAJAJ; Fiscal Year: 2000
    ..investigate the regulation of inducible tissue factor pathway inhibitor (TFPI) gene expression in monocytes and fibroblasts. Using Northern blotting analysis and TEPI assays, the applicant had previously demonstrated that endothelium ..
  74. Role of T cells and PPARgamma in Graves' orbital fibroblast adipogenesis
    Richard P Phipps; Fiscal Year: 2010
    ..TAO is disfiguring and may lead to blindness. Pre-adipocyte orbital fibroblasts are key effector cells in this process...
  75. Role of mechanical strain in incisional hernia fibroblast function.
    ERIC J CULBERTSON; Fiscal Year: 2010
    ..strain to signal repair, believed to occur through mechano-transduction pathways that activate wound repair fibroblasts. This proposal is founded on the premise that wound healing in load-bearing tissues is critically dependent on ..