chondrocytes

Summary

Summary: Polymorphic cells that form cartilage.

Top Publications

  1. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
  2. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
  3. pmc The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6
    Haruhiko Akiyama
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Genes Dev 16:2813-28. 2002
  4. pmc Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation
    B St-Jacques
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:2072-86. 1999
  5. ncbi Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source
    Yusuke Sakaguchi
    Tokyo Medical and Dental University, Japan
    Arthritis Rheum 52:2521-9. 2005
  6. ncbi Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors
    Stephen J Rodda
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 133:3231-44. 2006
  7. ncbi Isolation and characterization of mesenchymal stem cells from human umbilical cord blood: reevaluation of critical factors for successful isolation and high ability to proliferate and differentiate to chondrocytes as compared to mesenchymal stem cells fro
    Xiaohong Zhang
    Cell Therapy Research and Development Laboratory, New York Blood Center, New York, New York 10065, USA
    J Cell Biochem 112:1206-18. 2011
  8. ncbi Multipotent mesenchymal stem cells from adult human synovial membrane
    C De Bari
    Katholieke Universiteit Leuven, Belgium
    Arthritis Rheum 44:1928-42. 2001
  9. pmc Hypertrophic differentiation of chondrocytes in osteoarthritis: the developmental aspect of degenerative joint disorders
    Rita Dreier
    University Hospital of Munster, Institute for Physiological Chemistry and Pathobiochemistry, Waldeyerstra e 15, 48149 Munster, Germany
    Arthritis Res Ther 12:216. 2010
  10. pmc Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes
    Peter Dy
    Department of Cell Biology, Orthopaedic and Rheumatologic Research Center, Cleveland Clinic Lerner Research Institute, Cleveland, OH 44195, USA
    Dev Cell 22:597-609. 2012

Detail Information

Publications418 found, 100 shown here

  1. ncbi The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation
    Kazuhisa Nakashima
    Department of Molecular Genetics and Program in Genes and Development, M D Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cell 108:17-29. 2002
    ..Thus, Osx acts downstream of Runx2/Cbfa1. Because Osx null preosteoblasts express typical chondrocyte marker genes, we propose that Runx2/Cbfa1-expressing preosteoblasts are still bipotential cells...
  2. ncbi Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis
    Timothy F Day
    Geneti Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 8:739-50. 2005
    b>Chondrocytes and osteoblasts are two primary cell types in the skeletal system that are differentiated from common mesenchymal progenitors...
  3. pmc The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6
    Haruhiko Akiyama
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Genes Dev 16:2813-28. 2002
    ..Most cells were arrested as condensed mesenchymal cells and did not undergo overt differentiation into chondrocytes. Furthermore, chondrocyte proliferation was severely inhibited and joint formation was defective...
  4. pmc Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation
    B St-Jacques
    Harvard University, Department of Molecular and Cellular Biology, Cambridge, Massachusetts 02138 USA
    Genes Dev 13:2072-86. 1999
    ..Indian hedgehog (Ihh) signaling from prehypertrophic chondrocytes has been implicated in the control of chondrocyte maturation by way of feedback control of a second secreted ..
  5. ncbi Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source
    Yusuke Sakaguchi
    Tokyo Medical and Dental University, Japan
    Arthritis Rheum 52:2521-9. 2005
    ..To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue...
  6. ncbi Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors
    Stephen J Rodda
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 133:3231-44. 2006
    ..These data demonstrate that commitment within the osteoblast lineage requires sequential, stage-specific, Ihh and canonical Wnt/beta-catenin signaling to promote osteogenic, and block chondrogenic, programs of cell fate specification...
  7. ncbi Isolation and characterization of mesenchymal stem cells from human umbilical cord blood: reevaluation of critical factors for successful isolation and high ability to proliferate and differentiate to chondrocytes as compared to mesenchymal stem cells fro
    Xiaohong Zhang
    Cell Therapy Research and Development Laboratory, New York Blood Center, New York, New York 10065, USA
    J Cell Biochem 112:1206-18. 2011
    ..Functionally, CB-MSC could be more readily induced to differentiate into chondrocytes than could BM-MSC and AT-MSC. CB-MSC showed immunosuppressive activity equal to that of BM-MSC and AT-MSC...
  8. ncbi Multipotent mesenchymal stem cells from adult human synovial membrane
    C De Bari
    Katholieke Universiteit Leuven, Belgium
    Arthritis Rheum 44:1928-42. 2001
    ..SM-derived MSCs may play a role in the regenerative response during arthritic diseases and are promising candidates for developing novel cell-based therapeutic approaches for postnatal skeletal tissue repair...
  9. pmc Hypertrophic differentiation of chondrocytes in osteoarthritis: the developmental aspect of degenerative joint disorders
    Rita Dreier
    University Hospital of Munster, Institute for Physiological Chemistry and Pathobiochemistry, Waldeyerstra e 15, 48149 Munster, Germany
    Arthritis Res Ther 12:216. 2010
    ..In healthy articular cartilage, chondrocytes resist proliferation and terminal differentiation...
  10. pmc Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes
    Peter Dy
    Department of Cell Biology, Orthopaedic and Rheumatologic Research Center, Cleveland Clinic Lerner Research Institute, Cleveland, OH 44195, USA
    Dev Cell 22:597-609. 2012
    ..Sox9 protein outlives Sox9 RNA in upper hypertrophic chondrocytes, where it contributes with Mef2c to directly activate the major marker of these cells, Col10a1...
  11. ncbi Autologous chondrocyte implantation: a long-term follow-up
    Lars Peterson
    Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
    Am J Sports Med 38:1117-24. 2010
    ..The medium-term results of autologous chondrocyte implantation (ACI) have shown good to excellent outcomes for the majority of patients. However, no long-term results 10 to 20 years after the surgery have been reported...
  12. ncbi Histone deacetylase 4 controls chondrocyte hypertrophy during skeletogenesis
    Rick B Vega
    Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 119:555-66. 2004
    ..Here we report that HDAC4, which is expressed in prehypertrophic chondrocytes, regulates chondrocyte hypertrophy and endochondral bone formation by interacting with and inhibiting the ..
  13. pmc Integrative microRNA and proteomic approaches identify novel osteoarthritis genes and their collaborative metabolic and inflammatory networks
    Dimitrios Iliopoulos
    Institute of Biomedical Research and Technology, Larissa, Greece
    PLoS ONE 3:e3740. 2008
    ..Despite the high prevalence of the disease, the absence of large-scale molecular studies limits our ability to understand the molecular pathobiology of osteoathritis and identify targets for drug development...
  14. ncbi Articular cartilage and subchondral bone in the pathogenesis of osteoarthritis
    Mary B Goldring
    The Hospital for Special Surgery, Weill Cornell Medical College, New York, NY, USA
    Ann N Y Acad Sci 1192:230-7. 2010
    ..Although chondrocytes can modulate their functional state in response to loading, their capacity to repair and modify the surrounding ..
  15. pmc MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes
    T H Vu
    Department of Anatomy, University of California, San Francisco 94143, USA
    Cell 93:411-22. 1998
    ..Although hypertrophic chondrocytes develop normally, apoptosis, vascularization, and ossification are delayed, resulting in progressive ..
  16. ncbi Osteoarthritis cartilage histopathology: grading and staging
    K P H Pritzker
    Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Canada
    Osteoarthritis Cartilage 14:13-29. 2006
    ..Our objective was to devise a more useful method to assess OA histopathology that would have wide application for clinical and experimental OA assessment and would become recognized as the standard method...
  17. ncbi Endochondral ossification: how cartilage is converted into bone in the developing skeleton
    E J Mackie
    School of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia
    Int J Biochem Cell Biol 40:46-62. 2008
    ..During endochondral ossification, chondrocytes proliferate, undergo hypertrophy and die; the cartilage extracellular matrix they construct is then invaded by ..
  18. ncbi Bone development
    B R Olsen
    Harvard Medical School, Department of Cell Biology, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 16:191-220. 2000
    ..Within these condensations, cells differentiate to chondrocytes or osteoblasts and form cartilages and bones under the control of various transcription factors...
  19. ncbi Molecular and biophysical mechanisms regulating hypertrophic differentiation in chondrocytes and mesenchymal stem cells
    Deborah Studer
    Cartilage Engineering Regeneration Laboratory, Department of Health, Science and Technology, ETH Zurich, Switzerland
    Eur Cell Mater 24:118-35; discussion 135. 2012
    ..We hope this review will motivate the design of novel engineered microenvironments for skeletal regeneration applications...
  20. pmc Smooth muscle cells give rise to osteochondrogenic precursors and chondrocytes in calcifying arteries
    Mei Y Speer
    Department of Bioengineering, University of Washington, Box 355061, 1705 NE Pacific St Foege N330L, Seattle, WA 98195, USA
    Circ Res 104:733-41. 2009
    ....
  21. ncbi Cartilage biology in osteoarthritis--lessons from developmental biology
    Andrew A Pitsillides
    Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK
    Nat Rev Rheumatol 7:654-63. 2011
    ..The latest studies are summarized and we discuss the concepts emerging from these findings in cartilage biology, in the light of our understanding of the developmental processes involved...
  22. pmc Primary cilia mediate mechanotransduction through control of ATP-induced Ca2+ signaling in compressed chondrocytes
    Angus K T Wann
    School of Engineering and Materials Science, Queen Mary University of London, London, UK
    FASEB J 26:1663-71. 2012
    ..We generated conditionally immortalized wild-type (WT) and IFT88(orpk) (ORPK) mutant chondrocytes that lack primary cilia and assessed intracellular Ca(2+) signaling, extracellular matrix synthesis, and ATP ..
  23. ncbi Mechanical loading modulates chondrocyte primary cilia incidence and length
    Susan R McGlashan
    Department of Anatomy with Radiology, University of Auckland, Auckland, New Zealand
    Cell Biol Int 34:441-6. 2010
    The pathways by which chondrocytes of articular cartilage sense their mechanical environment are unclear. Compelling structural evidence suggests that chondrocyte primary cilia are mechanosensory organelles...
  24. pmc Type II collagen expression is regulated by tissue-specific miR-675 in human articular chondrocytes
    Katarzyna A Dudek
    The Kennedy Institute of Rheumatology, Imperial College London, 65 Aspenlea Road, London W6 8LH, United Kingdom
    J Biol Chem 285:24381-7. 2010
    ..However, the role of specific miRNAs in cartilage function is unknown. Using rarely available healthy human chondrocytes (obtained from 8 to 50 year old patients), we detected a most highly abundant primary miRNA H19, whose ..
  25. ncbi Sequential roles of Hedgehog and Wnt signaling in osteoblast development
    Hongliang Hu
    Department of Medicine, Washington University Medical School, St Louis, MO 63110, USA
    Development 132:49-60. 2005
    ..Finally Wnt7b is identified as a potential endogenous ligand regulating osteogenesis. These data support a model that integrates Hh and Wnt signaling in the regulation of osteoblast development...
  26. pmc Hypoxia in cartilage: HIF-1alpha is essential for chondrocyte growth arrest and survival
    E Schipani
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Genes Dev 15:2865-76. 2001
    ..In particular, we provide evidence that VEGF expression is up-regulated in a HIF-1alpha-independent manner in chondrocytes surrounding areas of cell death, and this in turn induces ectopic angiogenesis...
  27. pmc Biomechanical factors in osteoarthritis
    Farshid Guilak
    Departments of Orthopaedic Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    Best Pract Res Clin Rheumatol 25:815-23. 2011
    ..b>Chondrocytes perceive physical signals from their environment using a variety of mechanisms, including ion channels, ..
  28. ncbi Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration?
    P M van der Kraan
    Department of Rheumatology, Radboud University, Medical Centre, Geert Grooteplein 28, 6525 Nijmegen, The Netherlands
    Osteoarthritis Cartilage 20:223-32. 2012
    ..To review the literature on the role and regulation of chondrocyte terminal differentiation (hypertrophy-like changes) in osteoarthritis (OA) and to integrate this in a conceptual model of primary OA development...
  29. ncbi Role of chondrocyte death and hypocellularity in ageing human articular cartilage and the pathogenesis of osteoarthritis
    A Mobasheri
    Department of Veterinary Preclinical Sciences, Faculty of Veterinary Science, University of Liverpool, Brownlow Hill and Crown Street, Liverpool, L69 7ZJ UK
    Med Hypotheses 58:193-7. 2002
    Apoptotic death of articular chondrocytes has been implicated in the pathogenesis of human osteoarthritis...
  30. ncbi Gremlin 1, frizzled-related protein, and Dkk-1 are key regulators of human articular cartilage homeostasis
    J C H Leijten
    University of Twente, Enschede, The Netherlands
    Arthritis Rheum 64:3302-12. 2012
    The development of osteoarthritis (OA) may be caused by activation of hypertrophic differentiation of articular chondrocytes. Healthy articular cartilage is highly resistant to hypertrophic differentiation, in contrast to other hyaline ..
  31. pmc A novel form of chondrocyte stress is triggered by a COMP mutation causing pseudoachondroplasia
    Farhana Suleman
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom
    Hum Mutat 33:218-31. 2012
    ..Overall, these data suggest that a novel form of chondrocyte stress triggered by the expression of mutant COMP is central to the pathogenesis of PSACH...
  32. ncbi MicroRNAs regulate osteogenesis and chondrogenesis
    Shiwu Dong
    Laboratory of Biomechanics, Department of Anatomy, The Third Military Medical University, Chongqing, China
    Biochem Biophys Res Commun 418:587-91. 2012
    ..Some miRNAs regulate the proliferation and differentiation of osteoblasts, osteoclasts and chondrocytes, eventually influencing metabolism and bone formation...
  33. ncbi The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling
    Onju Ham
    Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120 752, Republic of Korea
    Biomaterials 33:4500-7. 2012
    ..not only H-89 or miR-23b-treated cells, but also cell co-treated with H-89 and miR-23b differentiated into chondrocytes. Our results indicate that H-89 induces the expression of endogenous miR-23b, thereby inducing chondrogenic ..
  34. ncbi Alarmins S100A8 and S100A9 elicit a catabolic effect in human osteoarthritic chondrocytes that is dependent on Toll-like receptor 4
    Rik F P Schelbergen
    Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Arthritis Rheum 64:1477-87. 2012
    ..The purpose of this study was to investigate whether S100A8 and/or S100A9 can interact with chondrocytes from OA patients to increase catabolic mediators.
  35. pmc MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates interleukin-1 responses
    Shigeru Miyaki
    Department of Molecular and Experimental Medicine, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Arthritis Rheum 60:2723-30. 2009
    ..The objectives of this study were to identify miRNA expressed in articular chondrocytes, to determine changes in osteoarthritic (OA) cartilage, and to address the function of miRNA-140 (miR-140).
  36. ncbi Hypoxia-inducible factor-2alpha is a catabolic regulator of osteoarthritic cartilage destruction
    Siyoung Yang
    Cell Dynamics Research Center, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea
    Nat Med 16:687-93. 2010
    ..HIF-2alpha directly induces the expression in chondrocytes of genes encoding catabolic factors, including matrix metalloproteinases (MMP1, MMP3, MMP9, MMP12 and MMP13), ..
  37. ncbi Maintenance of chondroitin sulfation balance by chondroitin-4-sulfotransferase 1 is required for chondrocyte development and growth factor signaling during cartilage morphogenesis
    Michael Klüppel
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Development 132:3989-4003. 2005
    ..Our study demonstrates an important biological role of differential chondroitin sulfation in mammalian development...
  38. pmc Dicer-dependent pathways regulate chondrocyte proliferation and differentiation
    Tatsuya Kobayashi
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 105:1949-54. 2008
    ..Dicer-null growth plates show a progressive reduction in the proliferating pool of chondrocytes, leading to severe skeletal growth defects and premature death of mice...
  39. ncbi The transcription factors L-Sox5 and Sox6 are essential for cartilage formation
    P Smits
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Dev Cell 1:277-90. 2001
    ..L-Sox5 and Sox6 are thus redundant, potent enhancers of chondroblast functions, thereby essential for endochondral skeleton formation...
  40. ncbi The surface of articular cartilage contains a progenitor cell population
    Gary P Dowthwaite
    Cardiff School of Biosciences and Cardiff Institute of Tissue Engineering and Repair, Cardiff University, PO Box 911, Museum Avenue, Cardiff CF10 3US, UK
    J Cell Sci 117:889-97. 2004
    ....
  41. pmc Runx2 and Runx3 are essential for chondrocyte maturation, and Runx2 regulates limb growth through induction of Indian hedgehog
    Carolina A Yoshida
    Department of Molecular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565 0871, Japan
    Genes Dev 18:952-63. 2004
    The differentiation of mesenchymal cells into chondrocytes and chondrocyte proliferation and maturation are fundamental steps in skeletal development...
  42. pmc Chronic infantile neurological cutaneous and articular syndrome is caused by mutations in CIAS1, a gene highly expressed in polymorphonuclear cells and chondrocytes
    Jerome Feldmann
    Unité de Recherche sur le Développement Normal et Pathologique du Système Immunitaire, INSERM U429, Hopital Necker Enfants Malades, Paris, France
    Am J Hum Genet 71:198-203. 2002
    ..A high level of expression of CIAS1 was found to be restricted to polymorphonuclear cells and chondrocytes. These findings demonstrate that CIAS1 missense mutations can result in distinct phenotypes with only a few ..
  43. ncbi The role of reactive oxygen species in homeostasis and degradation of cartilage
    Y E Henrotin
    Bone and Cartilage Research Unit, Institute of Pathology, Level 5, CHU Sart Tilman, Liege, Belgium
    Osteoarthritis Cartilage 11:747-55. 2003
    ..ROS production has been found to increase in joint diseases, such as osteoarthritis and rheumatoid arthritis, but their role in joint diseases initiation and progression remains questionable...
  44. pmc Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin
    Lin Gao
    Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Stem Cells 28:564-72. 2010
    ....
  45. ncbi Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects
    E B Hunziker
    M E Müller Institute for Biomechanics, University of Bern, Murtenstrasse 35, Switzerland
    Osteoarthritis Cartilage 10:432-63. 2002
    ..Current scientific trends and possible directions for the future will also be discussed...
  46. ncbi Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage
    Saifeddin Alsalameh
    Division of Arthritis Research MEM 161, The Scripps Research Institute, La Jolla, CA 92037, USA
    Arthritis Rheum 50:1522-32. 2004
    ..To determine the presence of mesenchymal progenitor cells (MPCs) in human articular cartilage...
  47. pmc Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage
    Amilton M Fernandes
    The Norwegian Center for Stem Cell Research, University of Oslo, Oslo, Norway
    PLoS ONE 8:e62994. 2013
    ..Patients with osteoarthritis (OA) may require tissue engineered cartilage therapy. Chondrocytes obtained from OA joints are thought to be involved in the disease process, and thus to be of insufficient ..
  48. pmc Epigenomic and microRNA-mediated regulation in cartilage development, homeostasis, and osteoarthritis
    Mary B Goldring
    Research Division, The Hospital for Special Surgery, Weill Cornell Medical College, New York, NY 10021, USA
    Trends Mol Med 18:109-18. 2012
    ..Herein, we summarize epigenetic and miRNA-mediated mechanisms impacting on OA progression and in this context offer potential therapeutic strategies for OA treatment...
  49. pmc TGF-beta/Smad3 signals repress chondrocyte hypertrophic differentiation and are required for maintaining articular cartilage
    X Yang
    Genetics of Development and Disease Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 153:35-46. 2001
    ..decreased production of proteoglycans, and abnormally increased number of type X collagen-expressing chondrocytes in synovial joints...
  50. ncbi p38 kinase regulates nitric oxide-induced apoptosis of articular chondrocytes by accumulating p53 via NFkappa B-dependent transcription and stabilization by serine 15 phosphorylation
    Song Ja Kim
    Department of Life Science, Kwangju Institute of Science and Technology, Gwangju 500 712, Korea
    J Biol Chem 277:33501-8. 2002
    Nitric oxide (NO) during primary culture of articular chondrocytes causes apoptosis via p38 mitogen-activated protein kinase in association with elevation of p53 protein level, caspase-3 activation, and differentiation status...
  51. ncbi Stromal cell-derived factor-1 induces matrix metalloprotease-13 expression in human chondrocytes
    Yung Cheng Chiu
    Department of Orthopaedics, Taichung Veterans General Hospital, Taichung, Taiwan
    Mol Pharmacol 72:695-703. 2007
    ..Here, we found that SDF-1alpha increased the secretion of MMP-13 in cultured human chondrocytes, as shown by reverse transcriptase-polymerase chain reaction, Western blot, and zymographic analysis...
  52. ncbi β-Defensin-4 (HBD-4) is expressed in chondrocytes derived from normal and osteoarthritic cartilage encapsulated in PEGDA scaffold
    Giuseppe Musumeci
    Department of Bio Medical Sciences, Anatomy Section, University of Catania, Italy
    Acta Histochem 114:805-12. 2012
    ..The aim of this study was to investigate β-defensin-4 expression in chondrocytes taken from articular cartilage of knees of patients with osteoarthritis (OA) compared to normal cartilage, in ..
  53. ncbi The matrilins: modulators of extracellular matrix assembly
    Andreas R Klatt
    Institute for Clinical Chemistry, University of Cologne, Germany
    Int J Biochem Cell Biol 43:320-30. 2011
    ....
  54. pmc A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources
    Tatiana Vinardell
    Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Dublin 2, Ireland
    Tissue Eng Part A 18:1161-70. 2012
    ..source for cartilage repair therapies that may overcome many of the problems associated with the use of primary chondrocytes (CCs)...
  55. pmc Characterization of microRNA expression profiles in normal and osteoarthritic human chondrocytes
    Silvia Díaz-Prado
    Department of Medicine, INIBIC University of A Coruña, A Coruna, Spain
    BMC Musculoskelet Disord 13:144. 2012
    ..The aim of this study was to identify and characterize the expression profile of miRNAs in normal and OA chondrocytes and to determine their role in the OA.
  56. ncbi C/EBPβ and RUNX2 cooperate to degrade cartilage with MMP-13 as the target and HIF-2α as the inducer in chondrocytes
    Makoto Hirata
    Sensory and Motor System Medicine, University of Tokyo, Hongo 7 3 1, Bunkyo ku, Tokyo 113 8655, Japan
    Hum Mol Genet 21:1111-23. 2012
    ..and a C/EBP motif-reporter assay identified RUNX2 as the most potent transcriptional partner of C/EBPβ in chondrocytes. C/EBPβ and RUNX2 were induced and co-localized in highly differentiated chondrocytes during the skeletal ..
  57. ncbi Molecular basis of anti-inflammatory action of platelet-rich plasma on human chondrocytes: mechanisms of NF-κB inhibition via HGF
    Paola Bendinelli
    Dipartimento di Morfologia Umana e Scienze Biomediche Città Studi, Molecular and Clinical Pathology Laboratories, Universita degli Studi di Milano, Milano, Italy
    J Cell Physiol 225:757-66. 2010
    ..First, activated PRP in chondrocytes reduced the transactivating activity of NF-κB, critical regulator of the inflammatory process, and decreased ..
  58. ncbi The treatment of chondral and osteochondral defects of the knee with autologous matrix-induced chondrogenesis (AMIC): method description and recent developments
    Jan Philipp Benthien
    Department of Orthopaedic Surgery, University of Basel, Basel, Switzerland
    Knee Surg Sports Traumatol Arthrosc 19:1316-9. 2011
    ..This technical note describes the method of autologous matrix-induced chondrogenesis (AMIC), a one-step procedure combining subchondral microfracture with the fixation of a collagen I/III membrane with fibrin glue or sutures...
  59. pmc Oxidative stress inhibits insulin-like growth factor-I induction of chondrocyte proteoglycan synthesis through differential regulation of phosphatidylinositol 3-Kinase-Akt and MEK-ERK MAPK signaling pathways
    Weihong Yin
    Department of Internal Medicine, Section of Molecular Medicine, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    J Biol Chem 284:31972-81. 2009
    ..We compared IGF-I signaling in normal and osteoarthritic human articular chondrocytes and investigated the effects of oxidative stress induced by tert-butylhydroperoxide (tBHP)...
  60. ncbi Mediation of spontaneous knee osteoarthritis by progressive chondrocyte ATP depletion in Hartley guinea pigs
    Kristen Johnson
    Veterans Affairs Medical Center, and University of California, San Diego, 92161, USA
    Arthritis Rheum 50:1216-25. 2004
    Because articular chondrocytes reside in a hypoxic milieu, anaerobic glycolysis is central in generating ATP to support chondrocyte matrix synthesis and viability, with mitochondrial oxidative phosphorylation possibly providing ..
  61. ncbi Adiponectin increases MMP-3 expression in human chondrocytes through AdipoR1 signaling pathway
    Kwok Man Tong
    Department of Orthopaedics, Taichung Veterans General Hospital, Taichung, Taiwan
    J Cell Biochem 112:1431-40. 2011
    ..We investigated the signaling pathway involved in MMP-3 caused by adiponectin in human chondrocytes. Adiponectin increased the secretion of MMP-3 in cultured human chondrocytes, as shown by qPCR, Western blot, ..
  62. ncbi Cytokines, tumor necrosis factor-alpha and interleukin-1beta, differentially regulate apoptosis in osteoarthritis cultured human chondrocytes
    M J Lopez-Armada
    Osteoarticular and Aging Research Unit, Rheumatology Division, C H Universitario Juan Canalejo, Xubias 84, 15006 A Coruna, Spain
    Osteoarthritis Cartilage 14:660-9. 2006
    This study addresses the effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) on cell death in human chondrocytes.
  63. pmc NF-kappaB signaling: multiple angles to target OA
    Kenneth B Marcu
    Biochemistry and Cell Biology Department, Stony Brook University, Stony Brook, NY 11794, USA
    Curr Drug Targets 11:599-613. 2010
    ..factors, OA pathogenesis also involves a "loss of maturational arrest" that inappropriately pushes chondrocytes towards a more differentiated, hypertrophic-like state...
  64. pmc Age-related changes in the musculoskeletal system and the development of osteoarthritis
    Richard F Loeser
    Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA
    Clin Geriatr Med 26:371-86. 2010
    ..Understanding the basic mechanisms by which aging affects joint tissues should provide new targets for slowing or preventing the development of OA...
  65. ncbi Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation
    Michael R Lang
    Department of Medicine, Division of Genetic Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
    Nat Genet 38:1198-203. 2006
    ..Zebrafish crusher mutants develop normally until the onset of craniofacial chondrogenesis. crusher chondrocytes accumulate proteins in a distended endoplasmic reticulum, resulting in severe reduction of cartilage ..
  66. ncbi Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential
    Alexandra Peister
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Blood 103:1662-8. 2004
    ..at very low density, formed single-cell-derived colonies, and readily differentiated into either adipocytes, chondrocytes, or mineralizing cells...
  67. ncbi Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis
    Atsushi Saito
    Division of Molecular and Cellular Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889 1692, Japan
    Nat Cell Biol 11:1197-204. 2009
    ..basic leucine zipper transcription factor, is activated in response to ER stress and is highly expressed in chondrocytes. In this study, we generated Bbf2h7(-/-) mice to assess the in vivo function of BBF2H7...
  68. ncbi Calcification of articular cartilage in human osteoarthritis
    M Fuerst
    Department of Orthopaedic Surgery, University Medical Center Hamburg Eppendorf, Clinic Bad Bramstedt, Martinistrasse 52, Hamburg, Germany
    Arthritis Rheum 60:2694-703. 2009
    ..The aim of this study was to determine the prevalence and composition of calcium crystals in human OA and analyze their relationship to disease severity and markers of chondrocyte hypertrophy...
  69. pmc miR-199a, a bone morphogenic protein 2-responsive MicroRNA, regulates chondrogenesis via direct targeting to Smad1
    Edward A Lin
    Department of Orthopaedic Surgery, New York University School of Medicine, New York, New York 10003, USA
    J Biol Chem 284:11326-35. 2009
    ..Taken together, miR-199a(*) is the first BMP2 responsive microRNA found to adversely regulate early chondrocyte differentiation via direct targeting of the Smad1 transcription factor...
  70. ncbi Treatment of symptomatic cartilage defects of the knee: characterized chondrocyte implantation results in better clinical outcome at 36 months in a randomized trial compared to microfracture
    Daniel B F Saris
    Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands
    Am J Sports Med 37:10S-19S. 2009
    ....
  71. pmc Vitamin D receptor in chondrocytes promotes osteoclastogenesis and regulates FGF23 production in osteoblasts
    Ritsuko Masuyama
    Laboratory of Experimental Medicine and Endocrinology, Katholieke Universiteit Leuven, Leuven, Belgium
    J Clin Invest 116:3150-9. 2006
    ..development and endochondral bone formation, we investigated mice with conditional inactivation of VDR in chondrocytes. Growth-plate chondrocyte development was not affected by the lack of VDR...
  72. ncbi Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1
    A Yamaguchi
    Department of Oral Pathology, Nagasaki University School of Dentistry, Sakamoto, Japan
    Endocr Rev 21:393-411. 2000
    Osteoblasts arise from common progenitors with chondrocytes, muscle and adipocytes, and various hormones and local factors regulate their differentiation...
  73. ncbi Cartilage engineering: a crucial combination of cells, biomaterials and biofactors
    Claire Vinatier
    INSERM, U 791, LIOAD, Nantes, F 44042, France
    Trends Biotechnol 27:307-14. 2009
    ..Finally, some of the major obstacles encountered in cartilage engineering are discussed, as well as future trends in clinical applications...
  74. ncbi Autologous chondrocyte implantation compared with microfracture in the knee. A randomized trial
    Gunnar Knutsen
    Department of Orthopaedic Surgery, University Hospital Tromsø, 9038 Tromsø, Norway
    J Bone Joint Surg Am 86:455-64. 2004
    ..The objective of the present study was to compare autologous chondrocyte implantation with microfracture in a randomized trial. We are not aware of any previous randomized studies comparing these methods...
  75. pmc Photodegradable hydrogels for dynamic tuning of physical and chemical properties
    April M Kloxin
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB ECCH 111, Boulder, CO 80309, USA
    Science 324:59-63. 2009
    ....
  76. ncbi SOX9 is a major negative regulator of cartilage vascularization, bone marrow formation and endochondral ossification
    Takako Hattori
    Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama City 700 8525, Japan
    Development 137:901-11. 2010
    ..In the foetal growth plate, Sox9 is highly expressed in chondrocytes of the proliferating and prehypertrophic zone but declines abruptly in the hypertrophic zone, suggesting that ..
  77. ncbi Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis
    Mehdi Shakibaei
    Institute of Anatomy, Ludwig Maximilians University Munich, Pettenkoferstrasse 11, D 80336 Munich, Germany
    Biochem Pharmacol 73:1434-45. 2007
    ..agent with potent anti-inflammatory properties on IL-1beta and TNF-alpha signalling pathways in human articular chondrocytes maintained in vitro...
  78. ncbi Regulation of inflammation signalling by resveratrol in human chondrocytes in vitro
    Constanze Csaki
    Ludwig Maximilians University Munich, Faculty of Medicine, Institute of Anatomy, Pettenkoferstrasse 11, Munich, Germany
    Biochem Pharmacol 75:677-87. 2008
    ..examined the effects of resveratrol on the proliferation and production of IL-1beta in primary human articular chondrocytes treated with IL-1betain vitro...
  79. ncbi Chondrocyte redifferentiation in 3D: the effect of adhesion site density and substrate elasticity
    Elena Schuh
    Institute for Biomechanics, ETH Zurich, Zurich, Switzerland
    J Biomed Mater Res A 100:38-47. 2012
    To obtain sufficient cell numbers for cartilage tissue engineering with autologous chondrocytes, cells are typically expanded in monolayer culture...
  80. ncbi MEF2C transcription factor controls chondrocyte hypertrophy and bone development
    Michael A Arnold
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Dev Cell 12:377-89. 2007
    ..These findings reveal unexpected commonalities in the mechanisms governing muscle, cardiovascular, and bone development with respect to their regulation by MEF2 and class II HDACs...
  81. ncbi Cell volume regulation in chondrocytes
    Rebecca Lewis
    Department of Musculoskeletal Biology, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UK
    Cell Physiol Biochem 28:1111-22. 2011
    b>Chondrocytes are the cells within cartilage which produce and maintain the extracellular matrix. Volume regulation in these cells is vital to their function and occurs in several different physiological and pathological contexts...
  82. ncbi Osteoarthritis
    Mary B Goldring
    Laboratory for Cartilage Biology, Research Division, The Hospital for Special Surgery, Weill College of Medicine of Cornell University, New York 10021, USA
    J Cell Physiol 213:626-34. 2007
    ..and synthesis of matrix proteins, proteinases, growth factors, cytokines, and other inflammatory mediators by chondrocytes, research has focused on the chondrocyte as the cellular mediator of OA pathogenesis...
  83. ncbi Aging theories of primary osteoarthritis: from epidemiology to molecular biology
    T Aigner
    Osteoarticular and Arthritis Research Group, Department of Pathology, University of Erlangen Nurnberg, Germany
    Rejuvenation Res 7:134-45. 2004
    ....
  84. ncbi Potent induction of chondrocytic differentiation of human adipose-derived adult stem cells by bone morphogenetic protein 6
    Bradley T Estes
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 54:1222-32. 2006
    ....
  85. ncbi A pair of Sox: distinct and overlapping functions of zebrafish sox9 co-orthologs in craniofacial and pectoral fin development
    Yi Lin Yan
    Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA
    Development 132:1069-83. 2005
    ..b>Chondrocytes failed to stack in sox9a mutants, failed to attain proper numbers in sox9b mutants and failed in both ..
  86. ncbi Strategies for articular cartilage lesion repair and functional restoration
    Tamer A E Ahmed
    Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada
    Tissue Eng Part B Rev 16:305-29. 2010
    ..e., mosaicplasty) or allogeneic osteochondral grafts, autologous chondrocytes (autologous chondrocyte transplantation), or tissue-engineered cartilage substitutes...
  87. ncbi Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification
    Makoto Hirao
    Department of Orthopaedics, Osaka University Graduate School of Medicine, 2 2 Yamadoaka, Suita, Osaka 565 0871, Japan
    J Biol Chem 281:31079-92. 2006
    ..These hypoxia-induced phenomena may act on chondrocytes to enhance and preserve their phenotype and function during chondrocyte differentiation and endochondral ..
  88. ncbi Platelet-rich plasma releasate inhibits inflammatory processes in osteoarthritic chondrocytes
    Gerben M van Buul
    Departments of Orthopaedics and Otorhinolaryngology, Erasmus MC, Dr Molewaterplein 50, Rotterdam, The Netherlands
    Am J Sports Med 39:2362-70. 2011
    ..Although anabolic effects of PRP on chondrocytes are well documented, no reports are known addressing effects on cartilage degeneration...
  89. pmc Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation
    Tilo Dehne
    Tissue Engineering Laboratory and Berlin Brandenburg Center for Regenerative Therapies, Department of Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Tucholskystrasse 2, Berlin, 10117, Germany
    Arthritis Res Ther 11:R133. 2009
    ..OA) are lacking an appropriate long-lasting treatment alternative, partly since it is not known if chondrocytes from OA patients have the same chondrogenic differentiation potential as chondrocytes from donors not affected ..
  90. ncbi Getting to the heart of the matter: osteoarthritis takes its place as part of the metabolic syndrome
    James D Katz
    Department of Medicine, The George Washington University, Washington, District of Columbia, USA
    Curr Opin Rheumatol 22:512-9. 2010
    ..It is now clear that an active genetic and proteomic profile suggests inflammation. The cytokine milieu is similarly inflammatory and neatly parallels that found in the metabolic syndrome...
  91. ncbi Peroxisome proliferator-activated receptor gamma in osteoarthritis
    Hassan Fahmi
    Osteoarthritis Research Unit, Notre Dame Hospital, University of Montreal Hospital Research Centre CRCHUM, 1560 Sherbrooke East, Pavillon J A DeSève, Y 2628, Montreal, QC, H2L 4M1, Canada
    Mod Rheumatol 21:1-9. 2011
    ..on the presence of PPARγ in articular tissues and its effect on inflammatory and catabolic responses in chondrocytes and synovial fibroblasts, as well as the protective effects of PPARγ ligands in arthritis experimental models...
  92. pmc FGFR3 signaling induces a reversible senescence phenotype in chondrocytes similar to oncogene-induced premature senescence
    Pavel Krejci
    Institute of Experimental Biology, Masaryk University, 61137 Brno, Czech Republic
    Bone 47:102-10. 2010
    ..Mutated FGFR3 induces constitutive activation of the ERK pathway in chondrocytes and, remarkably, can also cause both increased proliferation and apoptosis in growing cartilage, depending on ..
  93. ncbi Trophic effects of mesenchymal stem cells increase chondrocyte proliferation and matrix formation
    Ling Wu
    Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands
    Tissue Eng Part A 17:1425-36. 2011
    Previous studies showed that coculture of primary chondrocytes (PCs) with various sources of multipotent cells results in a higher relative amount of cartilage matrix formation than cultures containing only chondrocytes...
  94. ncbi Leptin enhances MMP-1, MMP-3 and MMP-13 production in human osteoarthritic cartilage and correlates with MMP-1 and MMP-3 in synovial fluid from OA patients
    Anna Koskinen
    The Immunopharmacology Research Group, Tampere University Hospital, Tampere, Finland
    Clin Exp Rheumatol 29:57-64. 2011
    ..In addition, the correlations between leptin and MMP concentrations in synovial fluid from OA patients were studied...
  95. pmc Effects of hyaluronic acid on mitochondrial function and mitochondria-driven apoptosis following oxidative stress in human chondrocytes
    Valentina Grishko
    Department of Cell Biology, University of South Alabama, Mobile, Alabama 36688, USA
    J Biol Chem 284:9132-9. 2009
    ..The exact mechanisms of its chondroprotective action are not yet fully elucidated. Human articular chondrocytes actively produce reactive oxygen and nitrogen species capable of causing cellular dysfunction and death...
  96. ncbi Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways
    Daisuke Ikegami
    Departments of Bone and Cartilage Biology, Osaka University Graduate School of Medicine, 2 2 Yamadaoka, Suita, Osaka, Japan
    Development 138:1507-19. 2011
    ..Sox9 is important in mesenchymal progenitors for their differentiation into chondrocytes, but its functions post-differentiation have not been determined...
  97. ncbi Simultaneous arthroscopic implantation of autologous chondrocytes and high tibial osteotomy for tibial chondral defects in the varus knee
    Francesco Franceschi
    Department of Orthopaedic and Trauma Surgery, Campus Biomedico University, Via Alvaro del Portillo, 200, 00128 Rome, Italy
    Knee 15:309-13. 2008
    ..the outcome of patients affected by these conditions, who underwent arthroscopic implantation of autologous chondrocytes and a medial opening wedge high tibial osteotomy...
  98. pmc Human circulating fibrocytes have the capacity to differentiate osteoblasts and chondrocytes
    Young H Choi
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Virginia School of Medicine, Charlottesville, VA 22908, United States
    Int J Biochem Cell Biol 42:662-71. 2010
    ..fibrocytes have the potential to differentiate into other mesenchymal lineage cells, such as osteoblasts and chondrocytes. In this study, we found that fibrocytes differentiated into osteoblast-like cells when cultured in osteogenic ..
  99. ncbi Characterization of articular cartilage and subchondral bone changes in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis
    Tadashi Hayami
    Department Molecular Endocrinology and Bone Biology, Merck Res Labs, West Point, PA 19486, USA
    Bone 38:234-43. 2006
    ..Together, these findings support a role for bone remodeling in OA pathogenesis and suggest that these rat models are suitable for evaluating bone resorption inhibitors as potential disease-modifying pharmaco-therapies...
  100. ncbi Differences in the secretome of cartilage explants and cultured chondrocytes unveiled by SILAC technology
    Martin Polacek
    Orthopaedic Surgery Department, Institute of Clinical Medicine, University of Tromsø, Breivika, Tromsø 9037, Norway
    J Orthop Res 28:1040-9. 2010
    ..main goal of our study was to analyze and compare the profiles of secreted proteins from adult human articular chondrocytes in monolayers, and cartilage explants in culture, using a de novo protein labeling approach...
  101. ncbi Primary culture and phenotyping of murine chondrocytes
    Marjolaine Gosset
    Paris Universitas Université Pierre et Marie Curie Paris VI, Department of Physiology and Physiopathology, Centre National de la Recherche Scientifique Unité Mixte de Recherche 7079, 7 quai St Bernard, Paris 75252 Cedex 5, France
    Nat Protoc 3:1253-60. 2008
    The culture of chondrocytes is one of the most powerful tools for exploring the intracellular and molecular features of chondrocyte differentiation and activation...

Research Grants77

  1. BBP (Bone Morphogenetic Protein Binding Peptide) and Bone Healing
    SAMUEL SCOTT MURRAY; Fiscal Year: 2013
    ..protein) cytokines stimulate pluripotent mesenchymal cells to proliferate and differentiate into the chondrocytes and osteoblasts that elaborate cartilage and bone...
  2. Novel Collagen II Alternative Transcripts and Mouse Skeletal Development
    Audrey McAlinden; Fiscal Year: 2010
    ..event where chondroprogenitor cells synthesize mainly type IIA mRNA, while differentiated chondrocytes produce mainly type IIB mRNA...
  3. Heat Shock Effects on Human Mesenchymal Stem Cell Differentiation in Hydrogel
    Sihong Wang; Fiscal Year: 2010
    ..2) to explore dynamic heat-shock effects on hMSC proliferation and differentiation into osteoblasts and chondrocytes in the optimized 3D culture environment, and to investigate the role of heat shock proteins (HSPs) in heat ..
  4. Hematopoietic Stem Cell Transplantation in Osteogenesis Imperfecta
    Meenal Mehrotra; Fiscal Year: 2012
    ..cells and osteoclasts, while MSCs generate a number of mesenchymal cells including fibroblasts, adipocytes, chondrocytes and osteoblasts...
  5. Thrombospondin 4 A1
    JOHN W LAWLER; Fiscal Year: 2010
    ..We have found that COMP binds to aggrecan, as well as to integrins in chondrocytes and fibroblasts. In addition, growth plate disorganization is observed in TSP-1-, TSP-3-, and COMP-null mice...
  6. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..COMP is expressed by chondro- cytes in endochondral bone formation, and continues to be expressed chondrocytes of mature cartilage...
  7. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2013
    ..factors play an important role in the normal homeostatic maintenance of the cartilage extracellular matrix by chondrocytes, and under abnormal conditions, mechanical stress may be a significant factor in the initiation and progression ..
  8. Elucidating the JNK2-mediated redox regulation of cartilage matrix remdeling
    ELIZABETH ALICE ERICKSON; Fiscal Year: 2010
    ..FN-f) stimulation of the a5[unreadable]1 integrin has been shown to increase intracellular levels of ROS in chondrocytes, while ROS inhibition blocked FN-f stimulated MMP-13 expression...
  9. Role of DDR2 in OA-like Pathogenesis in Osteochondrodysplasias
    Yefu Li; Fiscal Year: 2010
    ..in a surgically induced mouse OA model and in human hip OA cartilages;(5) increased MMP-13 expression in chondrocytes results from type II collagen-induced activation of DDR2 in vitro;(6) the Ras/Raf/MEK/ERK pathway is involved ..
  10. CHONDROPROTECTIVE ACTIVITY OF POMEGRANATE EXTRACT
    Tariq M Haqqi; Fiscal Year: 2010
    ..p38-MAPK and JNK, transcription factor NF-?B and the expression of MMPs by human cartilage explants and chondrocytes in vitro...
  11. Histone Deacetylation Regulates Growth Plate Development
    Lei Wei; Fiscal Year: 2013
    ..The last step in maturation of the growth plate is the differentiation of proliferative chondrocytes into hypertrophic chondrocytes, which subsequently undergo endochondral ossification...
  12. Identification of the transcriptional regulators of chondrocyte hypertrophy
    ANDREW BRUCE LASSAR; Fiscal Year: 2013
    ..e., Ihh and Collagen X) only if these cells are first induced to become chondrocytes by a variety of treatments, suggesting that a chondrogenic co-factor allows Runx2 to activate expression of ..
  13. Calcification and Vascular Cell Differentiation
    Cecilia M Giachelli; Fiscal Year: 2013
    ..medial calcification (MGP-/- mice), SMCs were found to be a major source of osteochondrogenic precursors and chondrocytes in the calcified vasculature...
  14. Regulation of guanylyl cyclase A and B by hormones, ATP and phosphorylation
    Lincoln Ross Potter; Fiscal Year: 2013
    ..Finally, the fourth aim will investigate how hormones inactivate GC- B in mouse follicles, granulosa cells, chondrocytes and smooth muscle cells by assessing the requirements for calcium elevations, PKC activation, and various ..
  15. MECHANISMS OF CHONDROPROTECTION BY POMEGRANATE FRUIT EXTRACT
    Tariq M Haqqi; Fiscal Year: 2011
    ..of p38-MAPK and JNK, NF-kB and expression of matrix metalloproteinases (MMPs) by human cartilage explants and chondrocytes in vitro. However, the mechanism of the inhibition of catabolic response remains elusive...
  16. Obesity, Biomechanics, and Inflammation in Osteoarthritis
    Farshid Guilak; Fiscal Year: 2013
    ..in combination with pro- inflammatory cytokines and fatty acids on the anabolic and catabolic activities of the chondrocytes, as measured by biomarker production, gene expression, and protein synthesis of collagen II and aggrecan...
  17. Mechanisms of Neural Crest Differentiation during Craniofacial Development
    Vivian M Lee; Fiscal Year: 2010
    ..Our preliminary results showed that many donor cells could differentiate into collagen II+ chondrocytes. This suggests that trunk neural crest has the capacity to differentiation into cartilage under certain ..
  18. Comparison of Bisphosphonate Treatment Regimens on Skeletal Growth &Biomechanics
    Marie Demay; Fiscal Year: 2013
    ..Hypophosphatemia impairs apoptosis of hypertrophic chondrocytes, both in vivo and in vitro, leading to the development of rickets in growing animals...
  19. ROS activation of Apoptosis Signal-regulated Kinase1 (Ask1) in chondrocytes
    Theresa Freeman; Fiscal Year: 2011
    ..In our preliminary data, we show Ask1 is present in growth plate and articular chondrocytes. We also show that hypoxia inducible factor 2 (HIF-2) is a transcriptional regulator controlling antioxidant ..
  20. Stem/progenitor cells of the chondrocyte and osteoblast lineage in vivo
    Noriaki Ono; Fiscal Year: 2013
    ..Specific Aim 1. Stem-like chondrocytes at the top of the postnatal epiphyseal growth plate cartilage: In endochondral bone formation, chondrocytes in ..
  21. Delivery of Soluble FGFR3 as a Treatment for Achondroplasia
    Steven C Ghivizzani; Fiscal Year: 2013
    ..interaction with FGF ligand acts negatively on both proliferation and terminal differentiation of growth plate chondrocytes. In 98% of those with achondroplasia, the phenotype is caused by a specific point mutation in FGFR3, resulting ..
  22. Function and Regulation of CD-RAP
    Linda J Sandell; Fiscal Year: 2013
    ..The disease process is characterized by the aberrant gene expression and the inability of cartilage chondrocytes to repair the extracellular matrix leading to cartilage degeneration...
  23. Gene enhanced cartilage repair in a cytokine permissive environment
    Alan J Nixon; Fiscal Year: 2012
    ..repair, cell survival, and transgene persistence in large full-thickness cartilage defects grafted with chondrocytes over expressing IGF-I...
  24. INTERPLAYS BETWEEN THE JAW MESENCHYMAL STEM CELLS AND T LYMPHOCYTES
    Yufang Shi; Fiscal Year: 2010
    ..are multipotent stem cells capable of differentiating into different lineage cells including osteoblasts, chondrocytes, adipocytes, cardiomyocytes, myoblasts, and neural cells...
  25. Role of Transcription Factor ERG in Skeletogenesis
    Maurizio Pacifici; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): During limb skeletogenesis, chondrocytes located at the epiphyseal ends of long bone anlagen develop into permanent articular chondrocytes that sustain joint function though life...
  26. FGF SIGNALING IN SKELETAL DEVELOPMENT, HOMEOSTASIS AND AGING
    David M Ornitz; Fiscal Year: 2013
    ..of function of FGF signaling in adult long bone osteoblasts and in skeletal progenitor cells that give rise to chondrocytes and osteoblasts in vivo. Using these genetic tools, our goals are to: 1...
  27. Role of Hypoxia in Differentiation
    Ernestina Schipani; Fiscal Year: 2012
    ..Our studies have shown that chondrocytes are an excellent model to learn how cells adapt to, and differentiate in a low oxygen environment...
  28. Lubricin Function in Articulating Joints
    Matthew L Warman; Fiscal Year: 2012
    ..our studies of the secreted protein lubricin (encoded by the gene PRG4), which is expressed by superficial zone chondrocytes and type B synoviocytes...
  29. Defining Common Molecular Parameters For Onset and Progression of Osteoarthritis
    Roland Wolkowicz; Fiscal Year: 2010
    ..GADD452, and DDR2, each of which impact on the expression of MMP-13, the major collagen-degrading enzyme in chondrocytes. Thus, our findings provide a conceptual framework that will be exploited to analyze unbiased genome-based and ..
  30. Transcriptional Regulation of Indian Hedgehog Expression in Chondrocytes
    Xiangli Yang; Fiscal Year: 2012
    ..2004). 2. Atf4-deficient (Atf4-/-) mice are dwarfs, suggesting a defect in growth plate chondrocytes. 3...
  31. Regulation of Skeletal Growth by Nuclear Retinoid Receptors
    Maurizio Pacifici; Fiscal Year: 2013
    ..skeletal structures in skull, trunk and limbs is governed by the activity of the growth plates where chondrocytes proliferate, undergo maturation and hypertrophy and are replaced by bone...
  32. Sox9-mediated control of chondrogenesis
    VERONIQUE M LEFEBVRE; Fiscal Year: 2013
    ..the redundant proteins Sox5 and Sox6 boost the ability of Sox9 to activate the differentiation program of early chondrocytes by increasing the efficiency of Sox9 binding to gene enhancers...
  33. Studies of Chondroyte Maturation
    Cristina C Teixeira; Fiscal Year: 2010
    ..b>Chondrocytes also play crucial roles in fracture repair and distraction osteogenesis...
  34. Novel Early Intervention to Prevent Post-Traumatic Osteoarthritis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..Preliminary data support our hypothesis. We demonstrate that: 1) the acute cellular response in primary human chondrocytes is almost completely abolished by ckd9 inhibitor, with >90% repression of 37 different IL-1? induced genes, ..
  35. PTHrP/IHH Microenvironment Control for Growth Plate Tissue Engineering
    JUAN MANUEL TABOAS; Fiscal Year: 2013
    ..that counter gradients of PTHrP and IHH agonists across a hydrogel construct seeded with MSC-derived chondrocytes will induce cell differentiation into a GP spatial architecture...
  36. Enhancement of Fracture Repair by Hematopoietic Stem Cells
    Amanda C LaRue; Fiscal Year: 2013
    ..EGFP+) hematopoietic stem cell (HSC), our data shows that the HSC gives rise to osteoblasts, osteocytes and chondrocytes during non-stabilized fracture repair...
  37. Drug Discovery for Multiple Hereditary Exostoses
    Jeffrey D Esko; Fiscal Year: 2013
    ..affects multiple signaling pathways through which growth factors regulate the organization and proliferation of chondrocytes in the growth plate...
  38. The role of microRNAs in chondrogenesis and arthritis
    Hiroshi Asahara; Fiscal Year: 2009
    ..appropriate control of chondrogenesis, a multistep process during which mesenchymal cells differentiate into chondrocytes. Articular cartilage destruction is a central factor in the pathogenesis of joint diseases, including ..
  39. Tissue Engineering of a Neotrachea
    James E Dennis; Fiscal Year: 2013
    ..Rabbit ear and shoulder chondrocytes will be tested and optimized for cartilage sheet production and will be tested for their long-term stability ..
  40. Role of microRNAs in skeletal development
    Tatsuya Kobayashi; Fiscal Year: 2013
    ..than miRNAs, it is not clear to what extent the loss of miRNAs contribute to the phenotype of Dicer-deficient chondrocytes. In Aim I, we will confirm the role of miRNAs in regulation of chondrocyte proliferation and differentiation by ..
  41. The use of genetic models to define the role of beta-catenin in post-natal growth
    REGIS J O'KEEFE; Fiscal Year: 2011
    ..Aim 3A examines regulation of BMP-2, 4, and 6 expressions in chondrocytes by ?-catenin. Aim 3B defines the cooperative induction of the VEGF gene by BMP-2 and ?-catenin...
  42. Shaping the craniofacial skeleton in the zebrafish
    APRIL F DELAURIER; Fiscal Year: 2010
    The objective of my project is to understand how chondrocytes, which make cartilage, and osteoblasts, which make bone, generate the specific elements of the zebrafish facial skeleton...
  43. ESE 1 a novel transcriptional regulator of cartilage remodeling
    MARY GOLDRING; Fiscal Year: 2009
    ..In inflammatory disorders, ESE-1 is expressed in multiple cell types, including chondrocytes, in response to interleukin-1 (IL-1) and other proinflammatory cytokines...
  44. Serum microRNAs as biomarkers of post-traumatic osteoarthritis
    Gary J Gibson; Fiscal Year: 2010
    ..We have demonstrated miRNA expression by cartilage and chondrocytes and the presence of miRNAs in human and mouse serum...
  45. Calcium-Sensing Receptors and Growth Plate Development
    Wenhan Chang; Fiscal Year: 2012
    ..is maintained by hormones, nutritional components, and autocrine and paracrine factors produced by chondrocytes. The concentration of Ca2+ in the extracellular fluid ([Ca2+]e) plays a key role in growth plate cartilage ..
  46. Pathogenic Mechanisms of Lysosomal Disease
    Richard Steet; Fiscal Year: 2013
    ..in the expression of ECM proteins and regulators of ECM synthesis and turnover impact specific properties of chondrocytes within developing cartilage...
  47. Suppression of MMP-13 Expression in Arthritis by Pomegranate
    Tariq M Haqqi; Fiscal Year: 2013
    ..In our preliminary studies we analyzed the global expression of miRNAs in human chondrocytes stimulated with IL-12 and discovered that miRNA mir-27b (mir-27b), with no known function, was downregulated ..
  48. Consequences of Mutant COMP Expression and Therapeutic Approaches in Transgenic M
    Jacqueline T Hecht; Fiscal Year: 2013
    ..This MT-COMP mouse shows intracellular growth plate pathology in vivo and the chondrocytes can be isolated and utilized for in vitro studies...
  49. The role of microRNAs in chondrogenesis and arthritis
    Hiroshi Asahara; Fiscal Year: 2013
    ..appropriate control of chondrogenesis, a multistep process during which mesenchymal cells differentiate into chondrocytes. Articular cartilage destruction is a central factor in the pathogenesis of joint diseases, including ..
  50. Oxidative Stress and the Development of Osteoarthritis
    Richard F Loeser; Fiscal Year: 2013
    ..We have found that in OA chondrocytes and in normal cells induced to exhibit oxidative stress, Akt activation is inhibited and this is associated ..
  51. The Role of Macrophage Migration Inhibitory Factor in Osteoarthritis
    MEREDITH A GREENE; Fiscal Year: 2013
    ..In Aim 2, we will use in vitro techniques to culture and stimulate chondrocytes, meniscal cells, and synovial fibroblasts with MIF, followed by protein and RNA analysis...
  52. Transglutaminase 2 in cartilage formation
    Maria Nurminskaya; Fiscal Year: 2011
    ..using MSCs for the articular cartilage repair faces a challenge of generating cells with features of stable chondrocytes which are resistant to hypertrophy and terminal differentiation...
  53. Selection of Therapeutic Agents for Articular Cartilage Repair
    Stephen B Trippel; Fiscal Year: 2013
    ..new cartilage formation to a greater degree than either class of agent alone Hypothesis 3B: Human articular chondrocytes respond to gene transfer in a fashion similar to that of bovine articular chondrocytes...
  54. ESE 1 a novel transcriptional regulator of cartilage remodeling
    Mary B Goldring; Fiscal Year: 2013
    ..that induce and activate ESE-1 to regulate MMP-13 and other targets? We will use primary mouse and human chondrocytes and cell lines to characterize the signaling and transcriptional mechanisms involved in the induction and ..
  55. Guanylyl cyclase receptors: Targets for medical intervention
    Joseph P Albanesi; Fiscal Year: 2010
    ..GCB is highly expressed in chondrocytes, adipocytes and fibroblasts;GCB activation usually leads to an inhibition of cell proliferation and hypertrophy...
  56. Cartilage Degeneration and Repair By ADAMTSs and Hyaluronan Binding Proteins
    Anna H Plaas; Fiscal Year: 2013
    ..In this paradigm, OA results from an "activation" of articular chondrocytes to the hypertrophic and autolytic phenotype which degrades the cartilage...
  57. Transgenic Approaches to Evaluate IGF-I Role in Peak BMD
    Subburaman Mohan; Fiscal Year: 2013
    ..led to an unexpected discovery that TH treatment causes several-fold increase in osterix (Osx) expression in chondrocytes in vitro and that Osx mRNA levels were severely compromised in bones of TH deficient mice compared to control ..
  58. The Role of JAB1 in osteosarcoma pathogenesis
    Guang Zhou; Fiscal Year: 2013
    ..Furthermore, Jab1-deficient primary chondrocytes exhibited accelerated apoptosis and altered cell cycle progression, suggesting a DNA-damage repair defect...
  59. Suppression of SHH Expression in Arthritis by Butea monosperma
    Tariq M Haqqi; Fiscal Year: 2013
    ..miR-323b- 5p), with no known function, was downregulated several fold in IL-1[unreadable]-stimulated chondrocytes. In silico analysis identified Sonic Hedgehog (SHH) mRNA as a target of miR-323b-5p...
  60. Mechanisms Underlying Hormonal Regulation of Fracture Repair
    Dolores M Shoback; Fiscal Year: 2013
    ..1 will explore the role of insulin-like growth factor-I (IGF-I) in coordinating the communication between chondrocytes, osteoblasts, and osteoclasts during the sequential process of fracture healing, looking first at specific ..
  61. Defining the molecular mechanisms underlying human RPE plasticity
    Sally Temple; Fiscal Year: 2013
    ..Moreover, primary adult human RPE that were clonally expanded from a single cell produced adipocytes, chondrocytes and bone when placed in the respective differentiation media...
  62. Osteosarcoma Translational Research
    Qiping Zheng; Fiscal Year: 2013
    ..However, besides osteoblasts, other cell types such as chondrocytes, adipocytes, and fibroblasts also appear within OS...
  63. HYALURONIC ACID/TGF-B COMPOSITE TO TREAT OSTEOARTHRITIS
    STEVEN KINCAID; Fiscal Year: 1991
    ..study will evaluate the efficacy of a composite of hyaluronic acid and TGF-B can: (1) stimulate dormant chondrocytes capable of matrix synthesis to regenerate atrophic articular cartilage to regenerate the cartilage lost in a ..
  64. PERLECAN AND CHONDROGENESIS
    Daniel Carson; Fiscal Year: 2004
    ..Precursor cells are recruited into a condensing core that, in turn, differentiates into chondrocytes. This differentiation process is accompanied by the elaboration of characteristic cartilage marker proteins ..
  65. COLLAGEN GENE EXPRESSION IN CHONDROCYTES IN INFLAMMATION
    MARY GOLDRING; Fiscal Year: 1990
    b>Chondrocytes are capable of participating in breakdown of their own matrix in response to inflammatory mediators and in repair of the damaged collagen network...
  66. PTHRP 1 to 173 IN CHONDROCYTES
    Robert Terkeltaub; Fiscal Year: 2005
    ..We have discovered that articular cartilage PTHrP expression becomes robust in osteoarthritis (OA) but that chondrocytes preferentially express the least widely expressed PTHrP isoform (PTHrP 1-1 73)...
  67. GFP Reporters for the chondrocyte lineage
    David Rowe; Fiscal Year: 2009
    ..This proposal is designed to introduce a new tool set of genetic markers for lineage studies in the chondrocytes that form the articular and growth plate cartilage...
  68. GLUCOSAMINE IN CARTILAGE HOMEOSTASIS AND DEGRADATION
    ALEXANDER SHIKHMAN; Fiscal Year: 2004
    ..N-acetylglucosamine (GlcNAc), express a broad range of anti-inflammatory activities in cultured human articular chondrocytes stimulated with interleukin-1beta (IL-1beta)...
  69. IL-8 in chondrocalcinosis
    RU BRYAN; Fiscal Year: 2005
    ..of C-X-C chemokines including IL-8 and its receptors CXCR-1 and CXCR-2 has recently been demonstrated in chondrocytes, and these mediators all are up regulated in OA cartilage in vivo...
  70. Gene Transfer of Growth Factors and Receptors as Therap*
    Ernest Terwilliger; Fiscal Year: 2002
    ..Cartilage is formed by cells called chondrocytes that surround themselves with a dense extracellular matrix, composed primarily of type 11 collagen, ..
  71. Design and Evaluation of Ultrasound Stimulation Aided Bioreactor Configurations
    Anu Subramanian; Fiscal Year: 2010
    ..bioreactor that utilizes stimulation by ultrasound, and conduct a detailed study on the effect of ultrasound on chondrocytes cultured in 3-D scaffolds in vitro...
  72. NOVEL GENE ASSOCIATED WITH CHONDROCYTE DIFFERENTIATION
    Paul Reynolds; Fiscal Year: 2001
    ..We identified Band 17 on the basis of differential expression between growth plate and articular chondrocytes, and have found no other tissues in which Band 17 is expressed as well as in growth plate chondrocytes...
  73. Examination of the Pre-pubertal Mammalian Growth Plate
    MICHELE REBECCA HUTCHISON; Fiscal Year: 2010
    ..Long bone growth in children is dependent on the constant and consistent proliferation and differentiation of chondrocytes at the growth plate...
  74. LIPIDS IN THE PATHOGENESIS OF OSTEOARTHROSIS
    Louis Lippiello; Fiscal Year: 1990
    Lipids are constant inclusions in chondrocytes of hyaline as well as other cartilages but their significance to chondrocyte function and metabolism in unknown...
  75. Chondroprogenitor cells in mature cartilage
    Martin Lotz; Fiscal Year: 2006
    ..Profound aging- associated changes in chondrocytes and cartilage extracellular matrix represent a major factor in OA pathogenesis...
  76. Resolution of Stage-Specific Growth Plate Chondrocytes
    Wenhan Chang; Fiscal Year: 2004
    b>Chondrocytes in the growth plate progress through steps of resting, proliferation, maturation, and hypertrophy and finally become terminally differentiated cells that produce a mature mineralized matrix and support bone formation...
  77. MECHANISMS & ENHANCEMENT OF INTEGRATIVE CARTILAGE REPAIR
    Robert Sah; Fiscal Year: 2004
    ..A corollary of this hypothesis is: transplanted chondrocytes can mediate and accelerate this process if these cells remain adherent at the defect site and contribute ..