muscle mitochondria

Summary

Summary: Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available.

Top Publications

  1. pmc AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation
    Haihong Zong
    Howard Hughes Medical Institute and the Departments of Internal Medicine, Cell Biology, and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 99:15983-7. 2002
  2. ncbi Skeletal muscle mitochondrial protein metabolism and function in ageing and type 2 diabetes
    Rocco Barazzoni
    Department of Clinical Morphological and Technological Sciences, Institute of Clinical Medicine, University of Trieste, Trieste, Italy
    Curr Opin Clin Nutr Metab Care 7:97-102. 2004
  3. ncbi Free radical generation by skeletal muscle of adult and old mice: effect of contractile activity
    A Vasilaki
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverppol L69 3GA, UK
    Aging Cell 5:109-17. 2006
  4. ncbi No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity
    David J Baker
    Faculty of Medicine, University of Calgary, Calgary, AB, T2N 1N4, Canada
    J Gerontol A Biol Sci Med Sci 61:675-84. 2006
  5. pmc Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo
    Catherine E Amara
    Department of Radiology, University of Washington Medical Center, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:1057-62. 2007
  6. pmc Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC-1alpha
    Zachary Gerhart-Hines
    Dana Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, One Jimmy Fund Way, Boston, MA 02115, USA
    EMBO J 26:1913-23. 2007
  7. ncbi Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency
    William A Irwin
    Department of Histology, University of Padova, 35121 Padova, Italy
    Nat Genet 35:367-71. 2003
  8. pmc Paradoxical effects of increased expression of PGC-1alpha on muscle mitochondrial function and insulin-stimulated muscle glucose metabolism
    Cheol Soo Choi
    Departments of Internal Medicine and Cellular and Molecular Physiology, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 105:19926-31. 2008
  9. pmc Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
    Clinton R Bruce
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 58:550-8. 2009
  10. ncbi Invited Review: contractile activity-induced mitochondrial biogenesis in skeletal muscle
    D A Hood
    Department of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    J Appl Physiol (1985) 90:1137-57. 2001

Detail Information

Publications310 found, 100 shown here

  1. pmc AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation
    Haihong Zong
    Howard Hughes Medical Institute and the Departments of Internal Medicine, Cell Biology, and Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 99:15983-7. 2002
    ..These data demonstrate that by sensing the energy status of the muscle cell, AMPK is a critical regulator involved in initiating mitochondrial biogenesis...
  2. ncbi Skeletal muscle mitochondrial protein metabolism and function in ageing and type 2 diabetes
    Rocco Barazzoni
    Department of Clinical Morphological and Technological Sciences, Institute of Clinical Medicine, University of Trieste, Trieste, Italy
    Curr Opin Clin Nutr Metab Care 7:97-102. 2004
    ..The current review will discuss advances in the understanding of skeletal muscle mitochondrial alterations associated with age and type 2 diabetes...
  3. ncbi Free radical generation by skeletal muscle of adult and old mice: effect of contractile activity
    A Vasilaki
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverppol L69 3GA, UK
    Aging Cell 5:109-17. 2006
    ..underwent a 15-min period of isometric contractions and we examined ROS generation by isolated skeletal muscle mitochondria, ROS release into the muscle extracellular fluid using microdialysis techniques, and the muscle glutathione ..
  4. ncbi No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity
    David J Baker
    Faculty of Medicine, University of Calgary, Calgary, AB, T2N 1N4, Canada
    J Gerontol A Biol Sci Med Sci 61:675-84. 2006
    ..However, there was a slower decline in PGC-1alpha gene expression with aging in CR versus AL animals, suggesting a better maintenance of mitochondrial biogenesis with aging in CR animals...
  5. pmc Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo
    Catherine E Amara
    Department of Radiology, University of Washington Medical Center, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:1057-62. 2007
    ..Instead, they support mild uncoupling as a mechanism protecting mitochondrial function and contributing to the paradoxical longevity of the most active muscle fibers...
  6. pmc Metabolic control of muscle mitochondrial function and fatty acid oxidation through SIRT1/PGC-1alpha
    Zachary Gerhart-Hines
    Dana Farber Cancer Institute and Department of Cell Biology, Harvard Medical School, One Jimmy Fund Way, Boston, MA 02115, USA
    EMBO J 26:1913-23. 2007
    ..These results have implications for understanding selective nutrient adaptation and how it might impact lifespan or metabolic diseases such as obesity and diabetes...
  7. ncbi Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency
    William A Irwin
    Department of Histology, University of Padova, 35121 Padova, Italy
    Nat Genet 35:367-71. 2003
    ..These findings indicate that collagen VI myopathies have an unexpected mitochondrial pathogenesis that could be exploited for therapeutic intervention...
  8. pmc Paradoxical effects of increased expression of PGC-1alpha on muscle mitochondrial function and insulin-stimulated muscle glucose metabolism
    Cheol Soo Choi
    Departments of Internal Medicine and Cellular and Molecular Physiology, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 105:19926-31. 2008
    ..This, in turn, caused activation of PKC, decreased insulin signaling at the level of insulin receptor substrate-1 (IRS-1) tyrosine phosphorylation, and skeletal muscle insulin resistance...
  9. pmc Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
    Clinton R Bruce
    Cellular and Molecular Metabolism Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia
    Diabetes 58:550-8. 2009
    ....
  10. ncbi Invited Review: contractile activity-induced mitochondrial biogenesis in skeletal muscle
    D A Hood
    Department of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    J Appl Physiol (1985) 90:1137-57. 2001
    ....
  11. ncbi High level of uncoupling protein 1 expression in muscle of transgenic mice selectively affects muscles at rest and decreases their IIb fiber content
    Elodie Couplan
    Ceremod CNRS UPR9078, 9 rue Jules Hetzel, 92190 Meudon, France
    J Biol Chem 277:43079-88. 2002
    ..This very likely constitutes the origin of the signal leading to the transition in fiber types at rest...
  12. ncbi Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis
    R Bergeron
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Am J Physiol Endocrinol Metab 281:E1340-6. 2001
    ..Our data suggest that AMPK may play an important role in muscle adaptations to chronic energy stress and that it promotes mitochondrial biogenesis and expression of respiratory proteins through activation of NRF-1...
  13. ncbi Peroxisomal-mitochondrial oxidation in a rodent model of obesity-associated insulin resistance
    Robert C Noland
    Department of Physiology, East Carolina University, Greenville, NC 27858, USA
    Am J Physiol Endocrinol Metab 293:E986-E1001. 2007
    ..These data suggest tissue-specific destinations for peroxisome-derived products and emphasize a potential role for peroxisomes in skeletal muscle lipid metabolism in the obese, insulin-resistant state...
  14. ncbi Cardiac and skeletal muscle mitochondria have a monocarboxylate transporter MCT1
    G A Brooks
    Exercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, California 94720 3140, USA
    J Appl Physiol (1985) 87:1713-8. 1999
    ..MCT1) in tissue lactate oxidation, isolated rat subsarcolemmal and interfibrillar cardiac and skeletal muscle mitochondria were probed with an antibody to MCT1...
  15. pmc Electron transport chain-dependent and -independent mechanisms of mitochondrial H2O2 emission during long-chain fatty acid oxidation
    Erin L Seifert
    Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada
    J Biol Chem 285:5748-58. 2010
    ..Thus, we utilized several complementary approaches to probe the underlying mechanism(s). Using skeletal muscle mitochondria, our findings indicate that even a low supply of LCFA is associated with ROS formation in excess of that ..
  16. pmc The deacetylase enzyme SIRT1 is not associated with oxidative capacity in rat heart and skeletal muscle and its overexpression reduces mitochondrial biogenesis
    Brendon J Gurd
    Department of Human Health and Nutritional Science, University of Guelph, Guelph, Ontario, Canada, N1G 2W1
    J Physiol 587:1817-28. 2009
    ..Taken altogether these experiments are not consistent with the notion that SIRT1 protein plays an obligatory regulatory role in the process of PGC-1alpha-mediated mitochondrial biogenesis in mammalian muscle...
  17. ncbi Toxicity of statins on rat skeletal muscle mitochondria
    P Kaufmann
    Division of Clinical Pharmacology and Toxicology and Department of Research, University Hospital, 4031, Basel, Switzerland
    Cell Mol Life Sci 63:2415-25. 2006
    ..In isolated rat skeletal muscle mitochondria, all statins, except pravastatin, decreased glutamate-driven state 3 respiration and respiratory control ..
  18. ncbi Reactive oxygen species from smooth muscle mitochondria initiate cold-induced constriction of cutaneous arteries
    S R Bailey
    Davis Heart and Lung Research Institute, R 110E, The Ohio State Univ, 473 West 12th Ave, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 289:H243-50. 2005
    ..Altered activity of ROS may contribute to cold-induced vasospasm occurring in Raynaud's phenomenon...
  19. pmc Molecular mechanisms of insulin resistance in humans and their potential links with mitochondrial dysfunction
    Katsutaro Morino
    Howard Hughes Medical Institute, Yale University School of Medicine, P O Box 9812, New Haven, CT 06536 8012, USA
    Diabetes 55:S9-S15. 2006
    ..By elucidating the cellular and molecular mechanisms responsible for insulin resistance, these studies provide potential new targets for the treatment and prevention of type 2 diabetes...
  20. pmc Hydrogen peroxide efflux from muscle mitochondria underestimates matrix superoxide production--a correction using glutathione depletion
    Jason R Treberg
    Buck Institute for Age Research, Novato, CA 94945, USA
    FEBS J 277:2766-78. 2010
    ..To assess this underestimate, we depleted matrix glutathione in rat skeletal muscle mitochondria by more than 90% as a consequence of pretreatment with 1-chloro-2,4-dintrobenzene (CDNB)...
  21. ncbi Sequential changes in superoxide production, anion carriers and substrate oxidation in skeletal muscle mitochondria of heat-stressed chickens
    Ahmad Mujahid
    Science of Biological Function, Life Science, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    FEBS Lett 581:3461-7. 2007
    We have shown that heat-stressed birds exhibit increased superoxide production in skeletal muscle mitochondria. To determine the precise mechanism for this effect, here we studied not only progressive, but also sequential changes in ..
  22. ncbi In obese rat muscle transport of palmitate is increased and is channeled to triacylglycerol storage despite an increase in mitochondrial palmitate oxidation
    Graham P Holloway
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
    Am J Physiol Endocrinol Metab 296:E738-47. 2009
    ..The additional fatty acid taken up is channeled primarily to esterification, suggesting that upregulation in fatty acid transport as opposed to altered fatty acid oxidation is the major determinant of intramuscular lipid accumulation...
  23. pmc Proteomics analysis of human skeletal muscle reveals novel abnormalities in obesity and type 2 diabetes
    Hyonson Hwang
    Center for Metabolic Biology, Arizona State University, Tempe, Arizona, USA
    Diabetes 59:33-42. 2010
    ..However, changes in mRNA levels may not predict changes in protein abundance. An approach to identify global protein abundance changes involving the use of proteomics was used here...
  24. pmc Functional assessment of isolated mitochondria in vitro
    Ian R Lanza
    Division of Endocrinology, Endocrinology Research Unit, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Methods Enzymol 457:349-72. 2009
    ....
  25. pmc High-fat diets cause insulin resistance despite an increase in muscle mitochondria
    Chad R Hancock
    Division of Geriatrics and Nutritional Sciences, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110
    Proc Natl Acad Sci U S A 105:7815-20. 2008
    ..this hypothesis, high-fat diets that cause insulin resistance have been reported to result in a decrease in muscle mitochondria. In contrast, we found that feeding rats high-fat diets that cause muscle insulin resistance results in a ..
  26. ncbi Expression profiling following local muscle inactivity in humans provides new perspective on diabetes-related genes
    James A Timmons
    Center for Genomics and Bioinformatics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Genomics 87:165-72. 2006
    ....
  27. ncbi Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells
    Andrey V Kuznetsov
    Daniel Swarovski Research Laboratory, Department of General and Transplant Surgery, Innsbruck Medical University, Innsbruck A6020, Austria
    Nat Protoc 3:965-76. 2008
    ..As only a small amount of tissue is required for analysis, the protocol can be used in diagnostic settings in clinical studies. The permeabilization procedure and specific titration analysis can be completed in 2 h...
  28. ncbi Differential susceptibility of subsarcolemmal and intermyofibrillar mitochondria to apoptotic stimuli
    Peter J Adhihetty
    School of Kinesiology and Health Science, York University, Farquharson Building, 302, Toronto, Ontario, Canada M3J 1P3
    Am J Physiol Cell Physiol 289:C994-C1001. 2005
    ....
  29. ncbi Cardiac and skeletal muscle fatty acid transport and transporters and triacylglycerol and fatty acid oxidation in lean and Zucker diabetic fatty rats
    Arend Bonen
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
    Am J Physiol Regul Integr Comp Physiol 297:R1202-12. 2009
    ..Upregulation of fatty acid transport increased red muscle, but not cardiac, triacylglycerol accumulation. White muscle lipid metabolism dysregulation was not observed...
  30. ncbi Effect of denervation on mitochondrially mediated apoptosis in skeletal muscle
    Peter J Adhihetty
    School of Kinesiology and Health Science, York University, Toronto, ON, Canada M3J 1P3
    J Appl Physiol 102:1143-51. 2007
    ....
  31. pmc Oxidative capacity and ageing in human muscle
    K E Conley
    Department of Radiology, University of Washington Medical Center, Seattle, WA 98195 7115, USA
    J Physiol 526:203-10. 2000
    ..The cellular basis of this drop was a reduction in mitochondrial content, as well as a lower oxidative capacity of the mitochondria with age...
  32. ncbi Characteristics of skeletal muscle mitochondrial biogenesis induced by moderate-intensity exercise and weight loss in obesity
    Elizabeth V Menshikova
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Appl Physiol 103:21-7. 2007
    ....
  33. ncbi Regulation of mitochondrial biogenesis in skeletal muscle by CaMK
    Hai Wu
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Science 296:349-52. 2002
    ..Thus, a calcium-regulated signaling pathway controls mitochondrial biogenesis in mammalian cells...
  34. pmc Decline in skeletal muscle mitochondrial function with aging in humans
    Kevin R Short
    Endocrine Research Unit and Department of Laboratory Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 102:5618-23. 2005
    ..These results demonstrate that age-related muscle mitochondrial dysfunction is related to reduced mtDNA and muscle functional changes that are common in the elderly...
  35. ncbi Lack of obesity and normal response to fasting and thyroid hormone in mice lacking uncoupling protein-3
    D W Gong
    Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 275:16251-7. 2000
    ..The phenotype of Ucp1/Ucp3 double knockout mice was indistinguishable from Ucp1 single knockout mice. These data suggest that Ucp3 is not a major determinant of metabolic rate but, rather, has other functions...
  36. pmc Calorie restriction increases muscle mitochondrial biogenesis in healthy humans
    Anthony E Civitarese
    Pennington Biomedical Research Center, Baton Rouge, Louisiana, United States of America
    PLoS Med 4:e76. 2007
    ..However, the mechanism responsible for this adaptation are poorly understood...
  37. ncbi Regulation of skeletal muscle mitochondrial fatty acid metabolism in lean and obese individuals
    Graham P Holloway
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
    Am J Clin Nutr 89:455S-62S. 2009
    ..These findings imply that obese individuals would benefit from interventions that increase the skeletal muscle mitochondrial content and the potential for oxidizing FAs...
  38. pmc Endurance training increases stimulation of uncoupling of skeletal muscle mitochondria in humans by non-esterified fatty acids: an uncoupling-protein-mediated effect?
    M Tonkonogi
    Department of Physiology and Pharmacology, Karolinska Institute, Box 5626, S 114 86 Stockholm, Sweden
    Biochem J 351:805-10. 2000
    Uncoupled respiration (UCR) is an essential property of muscle mitochondria and has several functions in the cell. We hypothesized that endurance training may alter the magnitude and properties of UCR in human muscle...
  39. pmc Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK
    Melina Fan
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:278-89. 2004
    ..The discovery of p160MBP as a PGC-1alpha regulator has important implications for the understanding of energy balance and diabetes...
  40. ncbi Identification of carbonylated proteins from enriched rat skeletal muscle mitochondria using affinity chromatography-stable isotope labeling and tandem mass spectrometry
    Danni L Meany
    Department of Chemistry, University of Minnesota, Minneapolis, MN, USA
    Proteomics 7:1150-63. 2007
    ..The results also demonstrate the utility of PQD operation on the LTQ instrument for quantitative analysis of iTRAQ reagent-labeled peptide mixtures, as well as the quantitative reproducibility of the avidin-affinity enrichment method...
  41. ncbi The effect of UCP3 overexpression on mitochondrial ROS production in skeletal muscle of young versus aged mice
    Miranda Nabben
    Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht, Maastricht University, PO Box 616, 6200 MD, Maastricht, The Netherlands
    FEBS Lett 582:4147-52. 2008
    ..State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production...
  42. ncbi Time course of ROS production in skeletal muscle mitochondria from chronic heat-exposed broiler chicken
    M A K Azad
    Nutritional Biochemistry of Animals, Graduate School of Agricultural Science, Tohoku University, 1 1 Tsutsumidori Amamiyamachi, Aoba ku, Sendai 981 8555, Japan
    Comp Biochem Physiol A Mol Integr Physiol 157:266-71. 2010
    This study was designed to elucidate physiological changes of skeletal muscle mitochondria from broiler chickens (Gallus gallus) during chronic heat exposure...
  43. pmc Effect of cyclosporin A and its vehicle on cardiac and skeletal muscle mitochondria: relationship to efficacy of the respiratory chain
    H Sanchez
    Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Sante des Armees, Ave du Maquis du Grésivaudan, 38702 La Tronche Cedex, France
    Br J Pharmacol 133:781-8. 2001
    ..The mechanism of action of the vehicle on the mitochondrial membrane and the implications for the clinical use of immunosuppressive drugs are discussed...
  44. ncbi Is ALS a systemic disorder? Evidence from muscle mitochondria
    Stanley H Appel
    Department of Neurology, Methodist Neurological Institute, Houston, TX 77030, USA
    Exp Neurol 198:1-3. 2006
  45. ncbi Mitochondrial electron transport chain function is enhanced in inspiratory muscles of patients with chronic obstructive pulmonary disease
    Florence Ribera
    Department of Physiology, Faculty of Medicine, 11 rue Humann, 67, 000 Strasbourg, France
    Am J Respir Crit Care Med 167:873-9. 2003
    ..Thus, severe emphysema was associated with increased mitochondrial capacity and efficiency in the inspiratory muscles, supporting an endurance training-like effect...
  46. pmc Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle
    Graham P Holloway
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada, N1G 2W1
    J Physiol 582:393-405. 2007
    ....
  47. ncbi Abnormal transition pore kinetics and cytochrome C release in muscle mitochondria of patients with chronic obstructive pulmonary disease
    Luis Puente-Maestu
    Servicio de Neumologia, Hospital General Universitario Gregorio Maranon, Madrid, Spain
    Am J Respir Cell Mol Biol 40:746-50. 2009
    ..Our objective was to study MPTP kinetics and cyto-c release in skeletal muscle mitochondria of patients with COPD...
  48. ncbi Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function
    Lisa S Chow
    Division of Endocrinology, Nutrition and Metabolism, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Appl Physiol 102:1078-89. 2007
    ....
  49. ncbi Coordinated and reversible reduction of enzymes involved in terminal oxidative metabolism in skeletal muscle mitochondria from a riboflavin-responsive, multiple acyl-CoA dehydrogenase deficiency patient
    Elisabetta Gianazza
    Dipartimento di Scienze Farmacologiche, Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Universita degli Studi, Milano, Italy
    Electrophoresis 27:1182-98. 2006
    ..Proteomic investigation of muscle mitochondria revealed decrease or absence of several flavoenzymes, enzymes related to flavin cofactor-dependent ..
  50. pmc Denervation induces cytosolic phospholipase A2-mediated fatty acid hydroperoxide generation by muscle mitochondria
    Arunabh Bhattacharya
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 3900, USA
    J Biol Chem 284:46-55. 2009
    ..Red dependent fluorescence (thought to be highly specific for hydrogen peroxide) compared with control muscle mitochondria. We now demonstrate that catalase only partially inhibits the Amplex Red signal in mitochondria from ..
  51. ncbi Flux control of cytochrome c oxidase in human skeletal muscle
    W S Kunz
    Department of Epileptology, University Bonn Medical Center, Sigmund Freud Strasse 25, D 53105 Bonn, Germany
    J Biol Chem 275:27741-5. 2000
    ....
  52. ncbi Mitochondrial function and apoptotic susceptibility in aging skeletal muscle
    Beatrice Chabi
    Department of Biology, York University, Toronto, Ontario, Canada
    Aging Cell 7:2-12. 2008
    ..These data suggest that the age-related sarcopenia and muscle fatigability are associated with enhanced ROS production, increased mitochondrial apoptotic susceptibility and reduced transcriptional drive for mitochondrial biogenesis...
  53. ncbi Quantitative and qualitative adaptation of human skeletal muscle mitochondria to hypoxic compared with normoxic training at the same relative work rate
    L Bakkman
    Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
    Acta Physiol (Oxf) 190:243-51. 2007
    ..To investigate if training during hypoxia (H) improves the adaptation of muscle oxidative function compared with normoxic (N) training performed at the same relative intensity...
  54. ncbi Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells
    Julie St-Pierre
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:26597-603. 2003
    ..Together, these data indicate that PGC-1alpha and PGC-1beta profoundly alter mitochondrial metabolism and suggest that these proteins are likely to play different physiological functions...
  55. ncbi Why are some mitochondria more powerful than others: insights from comparisons of muscle mitochondria from three terrestrial vertebrates
    Helga Guderley
    Helga Guderley, Dép de Biologie, Universite Laval, Quebec, P Q Canada
    Comp Biochem Physiol B Biochem Mol Biol 142:172-80. 2005
    We studied the molecular composition of muscle mitochondria to evaluate whether the contents of cytochromes or adenine nucleotide translocase (ANT) or phospholipid acyl compositions reflect differences in mitochondrial oxidative ..
  56. ncbi Exercise capacity and cytochrome oxidase activity in muscle mitochondria of COPD patients
    Bruno D'Agostino
    Dip di Medicina Sperimentale, Sez di Farmacologia, Facolta di Medicina e Chirurgia, Seconda Universita di Napoli, Italy
    Respir Med 104:83-90. 2010
    ..Nevertheless, further studies are required and a better understanding of the mechanism(s) underlying LABA effects might allow us to identify or unmask new therapeutic target(s) in such patients...
  57. ncbi Succinic dehydrogenase activity in human muscle mitochondria during aging: a quantitative cytochemical investigation
    P Fattoretti
    Neurobiology of Aging Laboratory, N. Masera INRCA Research Department, Via Birarelli 8, 60121 Ancona, Italy
    Mech Ageing Dev 122:1841-8. 2001
    ..In aging, energy-deficient sarcomeres are supported to be negatively selected and eliminated, while the surviving ones appear to maintain an adequate SDH activity...
  58. ncbi High rates of superoxide production in skeletal-muscle mitochondria respiring on both complex I- and complex II-linked substrates
    Florian L Muller
    Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center at San Antonio, Texas Research Park Campus, 15355 Lambda Drive, San Antonio, TX 78229 3900, USA
    Biochem J 409:491-9. 2008
    ....
  59. ncbi Increased malonyl-CoA levels in muscle from obese and type 2 diabetic subjects lead to decreased fatty acid oxidation and increased lipogenesis; thiazolidinedione treatment reverses these defects
    Gautam K Bandyopadhyay
    Department of Medicine, Division of Endocrinology and Metabolism, University of California at San Diego, 9500 Gilman Dr, La Jolla, 92093, USA
    Diabetes 55:2277-85. 2006
    ..In contrast, isolated muscle mitochondria from the type 2 subjects exhibited a greater rate of fatty acid oxidation compared with the lean group...
  60. pmc Raising plasma fatty acid concentration induces increased biogenesis of mitochondria in skeletal muscle
    Pablo Garcia-Roves
    Division of Geriatrics and Nutritional Science, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 104:10709-13. 2007
    ..We interpret our results as evidence that raising FFA induces an increase in mitochondrial biogenesis in muscle by activating PPARdelta...
  61. ncbi Absence of uncoupling protein-3 leads to greater activation of an adenine nucleotide translocase-mediated proton conductance in skeletal muscle mitochondria from calorie restricted mice
    Lisa Bevilacqua
    Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada KIH 8M5
    Biochim Biophys Acta 1797:1389-97. 2010
    ..of 40% CR demonstrated a rapid and sustained decrease in maximal leak-dependent respiration in skeletal muscle mitochondria. This was accompanied by decreased mitochondrial reactive oxygen species generation and increased ..
  62. ncbi The impact of the thermal sensitivity of cytochrome c oxidase on the respiration rate of Arctic charr red muscle mitochondria. pierre_blier@uqar.qc.ca
    P U Blier
    Departement de Biologie, Universite du Quebec a Rimouski, Canada
    J Comp Physiol B 171:247-53. 2001
    ..The functional link could be coevolution of parts of the mitochondrial system to maintain optimal functions in most of the temperature range encountered by organisms...
  63. pmc Muscle mitochondrial ATP synthesis and glucose transport/phosphorylation in type 2 diabetes
    Julia Szendroedi
    Department of Internal Medicine 3, University of Vienna, Vienna, Austria
    PLoS Med 4:e154. 2007
    ..We hypothesized that alterations in insulin action and mitochondrial function should be present even in nonobese patients with well-controlled type 2 diabetes mellitus (T2DM)...
  64. pmc Lower respiratory capacity in extraocular muscle mitochondria: evidence for intrinsic differences in mitochondrial composition and function
    Samir P Patel
    Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, KY 40536, USA
    Invest Ophthalmol Vis Sci 50:180-6. 2009
    ..The authors tested the hypothesis that extraocular muscle mitochondria respire faster than do mitochondria from limb muscles because of the higher content of respiratory complexes...
  65. ncbi MCT1 confirmed in rat striated muscle mitochondria
    C Eric Butz
    Department of Integrative Biology, University of California, Berkeley, California 94720 3140, USA
    J Appl Physiol 97:1059-66. 2004
    ..Probing with cytochrome oxidase showed mitochondrial localization of MCT1. Comparison of these results to the findings of others indicates that the most likely source of discrepancy is the cell fractionation procedure utilized...
  66. pmc Changes in skeletal muscle mitochondria in response to the development of type 2 diabetes or prevention by daily wheel running in hyperphagic OLETF rats
    R Scott Rector
    Harry S Truman Memorial VA Hospital, Division of Gastroenterology and Hepatology, University of Missouri, Columbia, MO 65201, USA
    Am J Physiol Endocrinol Metab 298:E1179-87. 2010
    ..Limited changes in skeletal muscle mitochondria were observed during the transition from insulin resistance to type 2 diabetes in the hyperphagic OLETF rat...
  67. ncbi T(3) increases mitochondrial ATP production in oxidative muscle despite increased expression of UCP2 and -3
    K R Short
    Endocrinology Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA
    Am J Physiol Endocrinol Metab 280:E761-9. 2001
    ..Despite large increases in UCP expression, ATP production was enhanced in oxidative tissues and maintained in glycolytic muscle of hyperthyroid rats...
  68. ncbi Activities of respiratory chain complexes and pyruvate dehydrogenase in isolated muscle mitochondria in premature neonates
    T Honzik
    Department of Paediatrics and Neonatology, Faculty of Medicine, Charles University, Ke Karlovu 2, Prague, Czech Republic
    Early Hum Dev 84:269-76. 2008
    ..Most diseases in premature neonates are secondary to immaturity of various organ systems. Also the inadequate capacity of mitochondrial energy production may play an important role in the neonatal morbidity...
  69. pmc Quantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondria
    Juan Feng
    University of Minnesota, 321 Church St SE, 6 155 Jackson Hall, Minneapolis, MN 55455, USA
    J Gerontol A Biol Sci Med Sci 63:1137-52. 2008
    Carbonylation is a highly prevalent protein modification in skeletal muscle mitochondria, possibly contributing to its functional decline with age...
  70. ncbi Redox state of endogenous coenzyme q modulates the inhibition of linoleic acid-induced uncoupling by guanosine triphosphate in isolated skeletal muscle mitochondria
    Wieslawa Jarmuszkiewicz
    Laboratory of Bioenergetics, Adam Mickiewicz University, Poznan, Poland
    J Bioenerg Biomembr 36:493-502. 2004
    The skeletal muscle mitochondria contain two isoforms of uncoupling protein, UCP2 and mainly UCP3, which had been shown to be activated by free fatty acids and inhibited by purine nucleotides in reconstituted systems...
  71. ncbi Measurement of ATP production and respiratory chain enzyme activities in mitochondria isolated from small muscle biopsy samples
    Rolf Wibom
    Centre for Inherited Metabolic Diseases, Department of Medical Laboratory Sciences and Technology, Karolinska Institutet, Huddinge University Hospital, S 141 86 Stockholm, Sweden
    Anal Biochem 311:139-51. 2002
    ..84-0.98; p<0.01). It is concluded that the set of methods is suitable for diagnosis of mitochondrial disorders in adults and small children...
  72. ncbi Plasticity of skeletal muscle mitochondria: structure and function
    Hans Hoppeler
    Department of Anatomy, University of Bern, Switzerland
    Med Sci Sports Exerc 35:95-104. 2003
    ..The molecular mechanisms that orchestrate the plasticity of skeletal muscle mitochondria are just beginning to unfold...
  73. ncbi Improved skeletal muscle oxidative enzyme activity and restoration of PGC-1 alpha and PPAR beta/delta gene expression upon rosiglitazone treatment in obese patients with type 2 diabetes mellitus
    M Mensink
    Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht NUTRIM, Maastricht University, Maastricht, The Netherlands
    Int J Obes (Lond) 31:1302-10. 2007
    ....
  74. ncbi Is mitochondrial dysfunction a cause of insulin resistance?
    Nigel Turner
    Diabetes and Obesity Research Program, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, NSW 2010, Australia
    Trends Endocrinol Metab 19:324-30. 2008
    ....
  75. pmc Impaired mitochondrial substrate oxidation in muscle of insulin-resistant offspring of type 2 diabetic patients
    Douglas E Befroy
    Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 8012, USA
    Diabetes 56:1376-81. 2007
    ....
  76. pmc New insights concerning the role of carnitine in the regulation of fuel metabolism in skeletal muscle
    Francis B Stephens
    Centre for Integrated Systems Biology and Medicine, School of Biomedical Sciences, Queen s Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK
    J Physiol 581:431-44. 2007
    ..The demonstration that its availability can be readily manipulated in humans, and impacts on physiological function, will result in renewed business and scientific interest in this compound...
  77. ncbi Control of mitochondrial beta-oxidation flux
    Simon Eaton
    Surgery Unit, Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK
    Prog Lipid Res 41:197-239. 2002
    ..The sharing of control between CPTI and other enzymes allows for flexible regulation of metabolism and the ability to rapidly adapt beta-oxidation flux to differing requirements in different tissues...
  78. ncbi The production of reactive oxygen and nitrogen species by skeletal muscle
    Malcolm J Jackson
    Division of Metabolic and Cellular Medicine, School of Clinical Sciences, University of Liverpool, Liverpool, United Kingdom
    J Appl Physiol (1985) 102:1664-70. 2007
    ..Full evaluation of the factors regulating these processes and the functions of the reactive oxygen species generated are important in understanding the redox biology of skeletal muscle...
  79. pmc Blood-feeding induces reversible functional changes in flight muscle mitochondria of Aedes aegypti mosquito
    Renata L S Goncalves
    Laboratório de Bioquímica Redox, Instituto de Bioquimica Medica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS ONE 4:e7854. 2009
    ..Here, we investigated the effects of blood-feeding on flight muscle (FM) mitochondria from the mosquito Aedes aegypti, a vector of dengue and yellow fever...
  80. pmc The role of AMP-activated protein kinase in mitochondrial biogenesis
    Richard M Reznick
    Department of Inernal Medicine, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, CT, USA
    J Physiol 574:33-9. 2006
    ..In addition, the potential role of mitochondrial dysfunction in the pathogenesis of insulin resistance associated with ageing and type 2 diabetes mellitus is also discussed...
  81. ncbi Absolute quantification of phosphorus metabolite concentrations in human muscle in vivo by 31P MRS: a quantitative review
    Graham J Kemp
    Division of Metabolic and Cellular Medicine, Faculty of Medicine, University of Liverpool, Liverpool, UK
    NMR Biomed 20:555-65. 2007
    ..e. from measured PCr/ATP and Pi/ATP ratios, assuming a literature value for [ATP])...
  82. pmc Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy
    Douglas P Millay
    Department of Pediatrics, University of Cincinnati, Cincinnati Children s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Nat Med 14:442-7. 2008
    ..Thus, mitochondrial-dependent necrosis represents a prominent disease mechanism in muscular dystrophy, suggesting that inhibition of cyclophilin D could provide a new pharmacologic treatment strategy for these diseases...
  83. ncbi PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein
    Graham P Holloway
    Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada
    Am J Physiol Endocrinol Metab 294:E1060-9. 2008
    ..Also, it does not appear that alterations in the proteins associated with mitochondrial network formation and degradation can account for the observed decrease in mitochondrial content...
  84. ncbi Aging skeletal muscle mitochondria in the rat: decreased uncoupling protein-3 content
    J Kerner
    Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Am J Physiol Endocrinol Metab 281:E1054-62. 2001
    ..in carnitine palmitoyltransferase I and carnitine palmitoyltransferase II activities in isolated skeletal muscle mitochondria. However, there was a small, significant decrease in tissue total carnitine content...
  85. pmc Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo
    Matthijs K C Hesselink
    Department of Movement Sciences, Nutrition and Toxicology Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
    J Clin Invest 111:479-86. 2003
    ..Taken together, our findings demonstrate that increasing muscle UCP3 expression does not diminish the rate of PCr resynthesis, allowing us to conclude that the primary role of UCP3 in humans is not uncoupling...
  86. ncbi Impaired in vivo mitochondrial function but similar intramyocellular lipid content in patients with type 2 diabetes mellitus and BMI-matched control subjects
    V B Schrauwen-Hinderling
    Department of Radiology, University Hospital Maastricht, P O Box 5800, 6202 AZ, Maastricht, The Netherlands
    Diabetologia 50:113-20. 2007
    ..As obesity is an independent determinant of IMCL content, we examined mitochondrial function and IMCL content in overweight type 2 diabetes patients and BMI-matched normoglycaemic controls...
  87. ncbi Effects of corticosterone on muscle mitochondria identifying different sensitivity to glucocorticoids in Lewis and Fischer rats
    Martine Duclos
    Laboratoire Neurogenetique et Stress, Institut National de la Santé et de la Recherche Médicale U471, Institut Francois Magendie, Universite Bordeaux II, 33077 Bordeaux, France
    Am J Physiol Endocrinol Metab 286:E159-67. 2004
    ..at concentrations in the range of chronic stress-induced levels presented a lower amount of functional muscle mitochondria with a decrease in cytochrome c oxidase and citrate synthase activities and a depletion of mitochondrial ..
  88. ncbi A novel potassium channel in skeletal muscle mitochondria
    Jolanta Skalska
    Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02 093 Warsaw, Poland
    Biochim Biophys Acta 1777:651-9. 2008
    In this work we provide evidence for the potential presence of a potassium channel in skeletal muscle mitochondria. In isolated rat skeletal muscle mitochondria, Ca(2+) was able to depolarize the mitochondrial inner membrane and ..
  89. pmc Insulin sensitivity and mitochondrial function are improved in children with burn injury during a randomized controlled trial of fenofibrate
    Melanie G Cree
    Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston 77555, USA
    Ann Surg 245:214-21. 2007
    ..To determine some of the mechanisms involved in insulin resistance immediately following burn trauma, and to determine the efficacy of PPAR-alpha agonism for alleviating insulin resistance in this population...
  90. ncbi Overexpression of carnitine palmitoyltransferase I in skeletal muscle in vivo increases fatty acid oxidation and reduces triacylglycerol esterification
    Clinton R Bruce
    Diabetes and Obesity Research Program, Garvan Institute of Medical Research, Darlinghurst
    Am J Physiol Endocrinol Metab 292:E1231-7. 2007
    ..05). These studies demonstrate that acute overexpression of CPT I in muscle leads to a repartitioning of FAs away from esterification and toward oxidation and highlight the importance of CPT I in regulating muscle FA metabolism...
  91. ncbi PPARgamma coactivator-1alpha expression during thyroid hormone- and contractile activity-induced mitochondrial adaptations
    Isabella Irrcher
    School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada M3J 1P3
    Am J Physiol Cell Physiol 284:C1669-77. 2003
    ....
  92. ncbi Characterization of superoxide production sites in isolated rat brain and skeletal muscle mitochondria
    Alexey P Kudin
    Department of Epileptology, University Bonn, Medical Center, Sigmund Freud Str 25, D 53105 Bonn, Germany
    Biomed Pharmacother 59:163-8. 2005
    ..report, we have quantified the superoxide and H(2)O(2) production rates of intact rat brain and skeletal muscle mitochondria under condition of oxygen saturation applying p-hydroxyphenylacetate as fluorescent probe for H(2)O(2) ..
  93. ncbi Mitochondria in skeletal muscle: adaptable rheostats of apoptotic susceptibility
    Peter J Adhihetty
    Department of Neurology and Neuroscience, Cornell University, New York, New York, USA
    Exerc Sport Sci Rev 36:116-21. 2008
    ..Exercise may attenuate some of the proapoptotic adaptations that occur during these conditions. This review will focus on the factors influencing mitochondrially mediated apoptosis in skeletal muscle...
  94. ncbi Effects of weight loss and physical activity on skeletal muscle mitochondrial function in obesity
    Elizabeth V Menshikova
    Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh, School of Medicine, Pennsylvania, USA
    Am J Physiol Endocrinol Metab 288:E818-25. 2005
    ..We conclude that improved skeletal muscle ETC activity following moderate WL and improved aerobic capacity contributes to associated alleviation of insulin resistance...
  95. ncbi Uncoupling protein-3: a new member of the mitochondrial carrier family with tissue-specific expression
    O Boss
    Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland
    FEBS Lett 408:39-42. 1997
    ..This new member of the UCP family is a candidate protein for the modulation of the respiratory control in skeletal muscle...
  96. ncbi Mitochondrial reactive oxygen species generation in obese non-diabetic and type 2 diabetic participants
    M A Abdul-Ghani
    Diabetes Division, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive MS 7886, San Antonio, TX 78229, USA
    Diabetologia 52:574-82. 2009
    ..The aim of this study was to measure mitochondrial reactive oxygen species (ROS) production directly from skeletal muscle biopsies obtained from obese insulin-resistant non-diabetic and type 2 diabetic participants...
  97. pmc Fatty acid oxidation in cardiac and skeletal muscle mitochondria is unaffected by deletion of CD36
    Kristen L King
    Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, USA
    Arch Biochem Biophys 467:234-8. 2007
    ..We isolated cardiac and skeletal muscle mitochondria from wild type and CD36 knock-out mice...
  98. pmc Control of muscle mitochondria by insulin entails activation of Akt2-mtNOS pathway: implications for the metabolic syndrome
    Paola Finocchietto
    Laboratory of Oxygen Metabolism, University Hospital, Buenos Aires, Argentina
    PLoS ONE 3:e1749. 2008
    ..We wondered whether insulin-operated signaling pathways modulate mitochondrial respiration via NO, to alternatively release complete glucose oxidation to CO(2) and H(2)O or to drive glucose storage to glycogen...
  99. pmc Rosiglitazone increases fatty acid oxidation and fatty acid translocase (FAT/CD36) but not carnitine palmitoyltransferase I in rat muscle mitochondria
    Carley R Benton
    Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    J Physiol 586:1755-66. 2008
    ..Moreover, these studies identify for the first time a mechanism by which rosiglitazone stimulates fatty acid oxidation in skeletal muscle, namely the chronic, subcellular relocation of FAT/CD36 to mitochondria...
  100. ncbi Effect of T(3)-induced hyperthyroidism on mitochondrial and cytoplasmic protein synthesis rates in oxidative and glycolytic tissues in rats
    Kevin R Short
    Endocrine Research Unit, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905, USA
    Am J Physiol Endocrinol Metab 292:E642-7. 2007
    ....
  101. ncbi Effect of chronic contractile activity on SS and IMF mitochondrial apoptotic susceptibility in skeletal muscle
    Peter J Adhihetty
    Department of Biology, School of Kinesiology and Health Science, York University, Toronto, ON, M3J 1P3, Canada
    Am J Physiol Endocrinol Metab 292:E748-55. 2007
    ..Our data suggest the possibility that chronic contractile activity can exert a protective effect on mitochondrially mediated apoptosis in muscle...

Research Grants62

  1. Lifespan Extension Despite Greatly Elevated Insulin and Body Fat
    David E Harrison; Fiscal Year: 2013
    ..Collaborators will test free radical damage to proteins of the electron transport chains for liver and muscle mitochondria. Aim 3...
  2. SIRT1 as a regulator of health and lifespan of mammals
    David A Sinclair; Fiscal Year: 2013
    ..reduced respiration and ATP, increased reactive oxygen species (ROS) and lactate, and a failure of muscle mitochondria to adapt their metabolism to fasting. Strikingly, this same defect occurs in wild type mice as they age...
  3. Macrophage Mitochondrial Stress in Inflammation, Insulin Resistance &Obesity
    LIZA MAKOWSKI HAYES; Fiscal Year: 2012
    ..my current research and work from our group has shown that obesity imposes a persistent lipid burden on muscle mitochondria, resulting in a mismatch between (-oxidation and tricarboxylic acid cycle (TCA) cycle activity...
  4. Regulation of Muscle ATP Synthase Beta Subunit Metabolism in Obesity
    Christos S Katsanos; Fiscal Year: 2013
    ..The abundance of the beta subunit of the ATP synthase ([unreadable] -F1-ATPase) in muscle mitochondria is decreased in obese, insulin-resistant individuals...
  5. MECHANISMS OF HUMAN INSULIN RESISTANCE
    W Timothy Garvey; Fiscal Year: 2010
    ..These studies will for the first time delineate functional and molecular defects in muscle mitochondria related to defects in substrate oxidation and IMCL in human insulin resistance.
  6. Race - adiposity interactions regulate mechanisms determining insulin sensitivity
    Michael J Quon; Fiscal Year: 2013
    ..third aim will test the hypothesis that greater bioenergetic efficiency and ROS production within skeletal muscle mitochondria will be associated with lower skeletal muscle insulin sensitivity in lean AA...
  7. Mechamism of Activation of Oxidative Metabolism in vivo During Exercise
    JESSICA ROSE SPIRES; Fiscal Year: 2012
    ..altered dehydrogenase activity) will be conducted at rest and during exercise to quantify the potential effects of reducing equivalent availability on citric acid cycle flux and oxidative phosphorylation in skeletal muscle mitochondria.
  8. Mechanisms linking the adipogenic phenotype of aging muscle to insulin resistance
    Deborah M Muoio; Fiscal Year: 2010
    ..further hypothesize that habitual exercise combats the adverse effects of lipid infiltration by remodeling muscle mitochondria in manner that enables these organelles to better cope with a high lipid load...
  9. MOLECULAR MECHANISMS OF OXIDATIVE STRESS IN AGING MUSCLE
    Christiaan Leeuwenburgh; Fiscal Year: 2012
    ..However, characterization of age-related changes to skeletal muscle mitochondria has been complicated by the realization that two distinct populations of mitochondria exist: interfibrillar ..
  10. SS peptides: improve mitochondrial and skeletal muscle function with age
    David J Marcinek; Fiscal Year: 2013
    ..This proposal tests a novel strategy for reversal of mitochondrial dysfunction in aged tissues that would represent a new paradigm for developing interventions to improve skeletal muscle function and quality of life in the elderly. ..
  11. Fat Cell Size, Overfeeding &Ectopic Fat
    Eric Ravussin; Fiscal Year: 2011
    ..We hypothesize that individuals with both a poor potential for adipose tissue expansion and an impaired function of muscle mitochondria will have the most deterioration of insulin sensitivity after 8 weeks of overfeeding.
  12. CARDIAC ENERGY METABOLISM IN HEART FAILURE
    Hani N Sabbah; Fiscal Year: 2013
    ..the molecular mechanisms for the decrease in oxidative phosphorylation found in heart and skeletal muscle mitochondria in HF...
  13. INDUCTION OF AMINOLEVULINIC ACID SYNTHASE BY EXERCISE
    David Essig; Fiscal Year: 1993
    Regularly performed endurance training results in a two-fold greater density of skeletal muscle mitochondria. Aminolevulinic acid synthase (AS), the rate limiting enzyme in heme biosynthesis, is increased two-fold in skeletal muscle ..
  14. DEVELOPMENTAL ASPECTS OF IRON NUTRITION
    PETER DALLMAN; Fiscal Year: 1991
    ..We plan to pursue evidence that the decreased oxidative capacity of skeletal muscle mitochondria in iron-deficient rats is associated with a decreased sensitivity to a respiratory signal, i.e...
  15. MOLECULAR GENETICS OF MATERNALLY TRANSMITTED DISEASE
    John Shoffner; Fiscal Year: 1993
    ..EBV-transformed lymphocytes from the patient are established and the OXPHOS defect is identified in muscle mitochondria by enzymatic analysis...
  16. Does UCP2/3 Overexpression Mimic Caloric Restriction?
    Barbara Horwitz; Fiscal Year: 2005
    ..In contrast, UCP3 knockouts have muscle mitochondria that generate more ROS and exhibit more oxidative damage...
  17. SKELETAL MUSCLE DYSFUNCTION IN COPD
    David Systrom; Fiscal Year: 2004
    ..Better understanding of the skeletal muscle oxidative abnormality in COPD will provide a rational basis for new therapies in this devastating disease. ..
  18. METABOLIC AND TRANSPORT PATHWAYS IN SKELETAL MUSCLE
    Robert Forster; Fiscal Year: 2004
    ..3) In muscle mitochondria measure the effect of CA V inhibition on gluconeogenesis and oxidative metabolism from pyruvate, supporting ..
  19. EXERCISE INDUCED BIOCHEMICAL AND ANATOMIC ADAPTATIONS
    John Holloszy; Fiscal Year: 2002
    ..The goal of this research is to discover the mechanisms by which exercise induces an increase in muscle mitochondria. Functional recognition sites for the transcription factors NRF-1 and/or NRF-2 have been identified in the ..
  20. Genetic Mechanisms of Acute Necrotizing Encephalopathy
    DEREK NEILSON; Fiscal Year: 2009
    ..In addition, we have performed biochemical studies of oxidative phosphorylation in isolated muscle mitochondria from one family member and have detected a loose coupling...
  21. CONTROL OF PROTEIN SYNTHESIS IN THE DIABETIC RAT
    DIANA BEATTIE; Fiscal Year: 1980
    ..rates of protein synthesis measured under optimal conditions in vitro were decreased over 60% in skeletal muscle mitochondria isolated from diabetic rats as compared to mitochondria from control rats...
  22. Regulation and metabolic impact of mitochondrial CD36 in skeletal muscle
    OLA MARTIN; Fiscal Year: 2006
    ..previously studied, only recently was it demonstrated that CD36 also participates in fatty acid uptake into muscle mitochondria. The physiological and pathophysiological relevance of this observation is still poorly understood...
  23. Aging of the subcellular muscle proteome
    Edgar Arriaga; Fiscal Year: 2005
    ..immuno-capture, and ICAT technology to identify (and discover) age-related carbonyl-associated proteins in muscle mitochondria. We will use the Fisher 344 rat as an animal model...
  24. Dietary Fat Mitochondria and Insulin Signaling
    Chad Hancock; Fiscal Year: 2007
    ..It is our hypothesis that the reduction in muscle mitochondria is due to physical inactivity and plays no role in modulating muscle insulin resistance...
  25. Plasma protein synthesis and abundance in T1 diabetes
    K Sreekumaran Nair; Fiscal Year: 2008
    ..These studies are anticipated to form the basis of more elaborate mechanistic studies in the future and hopefully will lead to alternative therapeutic approaches for T1 D. ..
  26. RESPIRATION AND GLYCOLYSIS--INTEGRATION IN ACTIVE MUSCLE
    Kevin Conley; Fiscal Year: 2002
    ..g., diabetes. An understanding of the integration of metabolism in healthy tissue is the first step to understanding how failure of integration limits function in diseased muscle. ..
  27. PATHOGENESIS OF SARCOPENIA AND METABOLIC CHANGE IN AGING
    K Nair; Fiscal Year: 2004
    ..This study will hopefully form the scientific basis for future trials of androgen replacement in the elderly. ..
  28. Mitochondrial Dysfunction in Aged Muscle
    David J Marcinek; Fiscal Year: 2010
    ..2) Our long-term goal is the development of non- invasive methods to diagnose mitochondrial dysfunction and follow the progress of interventions meant to reverse disability in the elderly. ..
  29. Physiological Consequences of Hypoxia and Lung Disease
    Peter Wagner; Fiscal Year: 2008
    ..abstract_text> ..
  30. Regulation of Skeletal Muscle LKB1
    WILLIAM WINDER; Fiscal Year: 2010
    ..The proposed studies will provide additional rationale for convincing a predominantly sedentary population of the importance of regular exercise in combating inactivity-related diseases. ..
  31. CLINICAL RESEARCH CENTER FOR NEUROMUSCULAR DISEASE
    Salvatore DiMauro; Fiscal Year: 2007
    ....
  32. Physiology of Malonyl-CoA in Muscle during Exercise
    WILLIAM WINDER; Fiscal Year: 2006
    ..The results will provide additional rationale for the use of regular exercise in preventing and treating type 2 diabetes and obesity. ..
  33. Control of ATP Producing Pathways in Skeletal Muscle
    Brandon Walsh; Fiscal Year: 2005
    ..determine the role of metabolic channeling in setting the onset kinetics of oxidative phosphorylation, and 3.) determine the intracellular PO2 at which respiration becomes O2 dependent. ..
  34. FUNCTION OF INTERMEDIATE FILAMENTS IN MUSCLE
    Yassemi Capetanaki; Fiscal Year: 2001
    ..In addition, the ability of the muscle to repair following injury will be examined. ..
  35. STATINS AND NON-CARDIAC ENDPOINTS
    Beatrice Golomb; Fiscal Year: 2003
    ....
  36. PROTON LEAK, OXIDATIVE STRESS, AND ENERGY RESTRICTION
    Jon Ramsey; Fiscal Year: 2003
    ..The premise is that Caloric Restriction (CR), hypothyroidism, and modulation of dietary fat will decrease mitochondrial permeability (i.e. proton leak), therefore decreasing molecular Oxygen consumption, and presumably oxidative stress. ..
  37. Developing antibodies to diagnose cerebral vasospasm
    Joseph Clark; Fiscal Year: 2003
    ..These data will provide proof of concept that antibodies against BOXes can be used to diagnose and predict vasospasm in SAH patients. ..
  38. High-force eccentric exercise for sarcopenia
    Stan Lindstedt; Fiscal Year: 2002
    ....
  39. Regulation of Mitochondrial Nitric-oxide Synthase
    Cecilia Giulivi; Fiscal Year: 2003
    ..Studying the biochemistry of mtNOS will provide a better understanding of the role of nitric oxide in the cell-mitochondria interactions relevant to physiological situations. ..
  40. Antioxidants and oxidative damage in mitochondria
    Cecilia Giulivi; Fiscal Year: 2002
    ..The antioxidant effect of flavonoids, coumarins, and catechins will be studied in terms hydroxyl substitution, conjugation of rings, and o- methylation of hydroxyl groups to elucidate an association between structure and function. ..
  41. AUTISM--A MODEL OF ANOMALOUS NEURAL SYSTEMS DEVELOPMENT
    Pauline Filipek; Fiscal Year: 2001
    ....
  42. Exercise Substrate Utilization:The Crossover Concept
    George Brooks; Fiscal Year: 2006
    ..Further, we have the auxiliary personnel and facilities to conduct longitudinal training studies ..
  43. Creatine Transport Across the Blood Brain Barrier
    Joseph Clark; Fiscal Year: 2006
    ..The goal for Hypothesis 2 is to develop methods and drugs to improve brain energy metabolism by getting creatine into the brain. ..
  44. Antioxidants and oxidative damage in mitochondria
    Cecilia Giulivi; Fiscal Year: 2004
    ..Structure-activity association of naturally occurring flavonoids in the abrogation of protein nitration, and 4. Bioavailability of flavonoids containing diets in nitrative stress. ..
  45. Dietary Protein Requirements in Hemodialysis Patients
    Joel Kopple; Fiscal Year: 2007
    ..Before and after these 5 months, total body protein will be assessed by TBNuf, body cell mass (TBK), and other components of body composition using anthropometry, DXA, and near infra-red interactance. ..
  46. Mitochrondrial Dysfunction: Role in Metabolic Syndrome
    Gerald Shulman; Fiscal Year: 2008
    ..The program is coordinated by frequent meetings of the program faculty and research workers bdnging together these diverse approaches to address a common goal. ..
  47. CALCIUM RELEASE UNITS IN CARDIAC MUSCLE
    Clara Franzini Armstrong; Fiscal Year: 2002
    ..3) The composition of CRUs in skeletal muscle of Amphioxus will be examined using molecular biology and ultrastructural approaches. ..
  48. MURINE MODEL OF CAG REPEAT DISEASES
    Roger Albin; Fiscal Year: 2002
    ..These experiments will establish HPRT-CAG mice as important tools for understanding neurodegeneration in CAG repeat diseases and enable accurate preclinical evaluation of interventions for CAG repeat diseases. ..
  49. EXERCISE AND SKELETAL MUSCLE FATTY ACID METABOLISM
    LORRAINE TURCOTTE; Fiscal Year: 2002
    ..For the first time, malonyl-CoA levels will be correlated to the rate of fatty acid oxidation in skeletal muscle. ..
  50. Evaluating Striatal Function in a Murine HD Model
    Roger Albin; Fiscal Year: 2007
    ..Validation of these methods will provide new and useful behavioral tools for evaluating murine disease models. [unreadable] [unreadable] [unreadable]..
  51. Bilirubin oxidation and intracerebral hemorrhage
    Joseph Clark; Fiscal Year: 2009
    ..These studies are important because we believe that strategies designed to prevent BOXes production in the hematoma should provide a novel and beneficial therapeutic option for patients who have suffered an ICH. ..
  52. 2007 Aging, Biology of Gordon Conference
    LaDora Thompson; Fiscal Year: 2007
    ..We fully anticipate that progress in this field will pave the way for interventions and quality of life for the older adult. [unreadable] [unreadable] [unreadable] [unreadable]..
  53. Mitochondrial sites of reactive oxygen species generation in cells.
    Martin D Brand; Fiscal Year: 2010
    ....
  54. Coenzyme Q10 in Aging: A Planning Grant
    Beatrice Golomb; Fiscal Year: 2005
    ..Thus there is strong conceptual as well as clinical rationale for assessing whether Q10 provides QOL & functional benefits in aging and whether it protects against untoward events associated with age. ..
  55. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....
  56. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
    ..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
  57. EXCITATION-CONTRACTION COUPLING IN STRIATED MUSCLE
    STEPHEN BAYLOR; Fiscal Year: 2005
    ..Overall, the knowledge obtained will contribute importantly to understanding the mechanisms of intracellular Ca signaling and the mechanisms by which specific drugs and diseases affect muscle performance. ..