liver mitochondria

Summary

Summary: Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)

Top Publications

  1. ncbi Changes in rat liver mitochondria with aging. Lon protease-like reactivity and N(epsilon)-carboxymethyllysine accumulation in the matrix
    Hilaire Bakala
    Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Université Paris7 Denis Diderot, Paris, France
    Eur J Biochem 270:2295-302. 2003
  2. ncbi The Ala16Val genetic dimorphism modulates the import of human manganese superoxide dismutase into rat liver mitochondria
    Angela Sutton
    Institut National de la Santé et de la Recherche Médicale Unité 481, Hopital Beaujon, Clichy, France
    Pharmacogenetics 13:145-57. 2003
  3. ncbi Species- and tissue-specific relationships between mitochondrial permeability transition and generation of ROS in brain and liver mitochondria of rats and mice
    Alexander Panov
    Carolinas Neuromuscular ALS MDA Center, Carolinas Medical Center, 1000 Blythe Blvd, Charlotte, NC 28203, USA
    Am J Physiol Cell Physiol 292:C708-18. 2007
  4. pmc Proteome, phosphoproteome, and hydroxyproteome of liver mitochondria in diabetic rats at early pathogenic stages
    Wen Jun Deng
    Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Mol Cell Proteomics 9:100-16. 2010
  5. ncbi Distinct characteristics of Ca(2+)-induced depolarization of isolated brain and liver mitochondria
    Olga Vergun
    Department of Pharmacology, University of Pittsburgh, W1351 Biomedical Science Tower, Pittsburgh, PA 15261, USA
    Biochim Biophys Acta 1709:127-37. 2005
  6. ncbi Effects of fasting on oxidative stress in rat liver mitochondria
    M Sorensen
    Danish Center for Molecular Gerontology, University of Aarhus, Department of Molecular Biology, 8000 Aarhus, Denmark
    Free Radic Res 40:339-47. 2006
  7. ncbi Tetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stress
    Maria A S Fernandes
    Departamento de Zoologia, Faculdade de Farmacia, Universidade de Coimbra, Couraça dos apóstolos, 51, R C, 3000 517 Coimbra, Portugal
    Mitochondrion 6:176-85. 2006
  8. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
  9. ncbi HO-1 is located in liver mitochondria and modulates mitochondrial heme content and metabolism
    Daniela P Converso
    Laboratory of Oxygen Metabolism, University Hospital, University of Buenos Aires, Argentina
    FASEB J 20:1236-8. 2006
  10. ncbi Biased incorporation of ribonucleotides on the mitochondrial L-strand accounts for apparent strand-asymmetric DNA replication
    Ming Yao Yang
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, United Kingdom
    Cell 111:495-505. 2002

Detail Information

Publications333 found, 100 shown here

  1. ncbi Changes in rat liver mitochondria with aging. Lon protease-like reactivity and N(epsilon)-carboxymethyllysine accumulation in the matrix
    Hilaire Bakala
    Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Université Paris7 Denis Diderot, Paris, France
    Eur J Biochem 270:2295-302. 2003
    ....
  2. ncbi The Ala16Val genetic dimorphism modulates the import of human manganese superoxide dismutase into rat liver mitochondria
    Angela Sutton
    Institut National de la Santé et de la Recherche Médicale Unité 481, Hopital Beaujon, Clichy, France
    Pharmacogenetics 13:145-57. 2003
    ..dihydrofolate reductase (DHFR) protein, and the import of the two human MnSOD precursor variants into rat liver mitochondria. Compared to Ala-proteins, the Val-MnSOD/MTS-DHFR precursor and Val-MnSOD precursor were both partly ..
  3. ncbi Species- and tissue-specific relationships between mitochondrial permeability transition and generation of ROS in brain and liver mitochondria of rats and mice
    Alexander Panov
    Carolinas Neuromuscular ALS MDA Center, Carolinas Medical Center, 1000 Blythe Blvd, Charlotte, NC 28203, USA
    Am J Physiol Cell Physiol 292:C708-18. 2007
    ..Thus the observed differences in the Ca(2+)-induced mPT and ROS generation may underlie both the organ-specific and species-specific variability in the animal models of neurodegenerative diseases...
  4. pmc Proteome, phosphoproteome, and hydroxyproteome of liver mitochondria in diabetic rats at early pathogenic stages
    Wen Jun Deng
    Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    Mol Cell Proteomics 9:100-16. 2010
    ..To dissect the underlying mechanisms, we performed a multiplexed proteomics study on liver mitochondria isolated from a spontaneous diabetic rat model before/after they were rendered diabetic...
  5. ncbi Distinct characteristics of Ca(2+)-induced depolarization of isolated brain and liver mitochondria
    Olga Vergun
    Department of Pharmacology, University of Pittsburgh, W1351 Biomedical Science Tower, Pittsburgh, PA 15261, USA
    Biochim Biophys Acta 1709:127-37. 2005
    Ca(2+)-induced mitochondrial depolarization was studied in single isolated rat brain and liver mitochondria. Digital imaging techniques and rhodamine 123 were used for mitochondrial membrane potential measurements...
  6. ncbi Effects of fasting on oxidative stress in rat liver mitochondria
    M Sorensen
    Danish Center for Molecular Gerontology, University of Aarhus, Department of Molecular Biology, 8000 Aarhus, Denmark
    Free Radic Res 40:339-47. 2006
    ..Different parameters related to lipid and protein oxidative damage were also analyzed. Liver mitochondria from rats subjected to 72 h of fasting leaked more electrons per unit of O(2) consumed at complex III, than ..
  7. ncbi Tetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stress
    Maria A S Fernandes
    Departamento de Zoologia, Faculdade de Farmacia, Universidade de Coimbra, Couraça dos apóstolos, 51, R C, 3000 517 Coimbra, Portugal
    Mitochondrion 6:176-85. 2006
    ..were assessed on oxidative stress, mitochondrial permeability transition (MPT), and bioenergetics of rat liver mitochondria. At concentrations lower than 100 nmol/mg protein, tetrandrine decreased the hydrogen peroxide formation, ..
  8. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..Our results indicate that the Bcl-2 family of proteins bind to the VDAC in order to regulate the mitochondrial membrane potential and the release of cytochrome c during apoptosis...
  9. ncbi HO-1 is located in liver mitochondria and modulates mitochondrial heme content and metabolism
    Daniela P Converso
    Laboratory of Oxygen Metabolism, University Hospital, University of Buenos Aires, Argentina
    FASEB J 20:1236-8. 2006
    ....
  10. ncbi Biased incorporation of ribonucleotides on the mitochondrial L-strand accounts for apparent strand-asymmetric DNA replication
    Ming Yao Yang
    MRC Dunn Human Nutrition Unit, Hills Road, CB2 2XY, Cambridge, United Kingdom
    Cell 111:495-505. 2002
    ..Mammalian mitochondrial DNA replication thus proceeds mainly, or exclusively, by a strand-coupled mechanism...
  11. ncbi Cloning of human cDNAs encoding mitochondrial and cytosolic serine hydroxymethyltransferases and chromosomal localization
    T A Garrow
    Department of Nutritional Sciences, University of California, Berkeley 94720
    J Biol Chem 268:11910-6. 1993
    ..The high degree of nucleotide sequence identity between the two isozymes, and the presence of keratin genes in both chromosomal regions, is consistent with these regions of chromosome 12 and 17 arising by a duplication event...
  12. ncbi Dioxin increases reactive oxygen production in mouse liver mitochondria
    Albert P Senft
    Department of Environmental Health and Center for Environmental Genetics, University of Cincinnati Medical Center, Cincinnati, Ohio 45267 0056, USA
    Toxicol Appl Pharmacol 178:15-21. 2002
    ..Female C57BL/6 mice were treated with dioxin (15 microg/kg body wt ip) on 3 consecutive days, and liver mitochondria were examined at 1, 4, and 8 weeks after the first treatment...
  13. ncbi Hepatic mitochondrial glutathione: transport and role in disease and toxicity
    Jose C Fernandez-Checa
    Liver Unit, Hospital Clinic i Provincial, Instituto Investigaciones Biomedicas August Pi i Sunyer, Spain
    Toxicol Appl Pharmacol 204:263-73. 2005
    ..Evidence mainly from kidney and liver mitochondria indicated that the dicarboxylate and the 2-oxoglutarate carriers contribute to the transport of GSH across ..
  14. ncbi c-Jun N-terminal kinase plays a major role in murine acetaminophen hepatotoxicity
    Basuki K Gunawan
    Research Center for Liver Disease, Keck School of Medicine, University of Southern California, Los Angeles, 90033, USA
    Gastroenterology 131:165-78. 2006
    ..In searching for effects of acetaminophen (APAP) on hepatocytes downstream of its metabolism that may participate in hepatotoxicity, we examined the role of stress kinases...
  15. ncbi Reversible glutathionylation of complex I increases mitochondrial superoxide formation
    Ellen R Taylor
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Bldg, Hills Rd, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:19603-10. 2003
    ....
  16. ncbi Toxicity of bile acids on the electron transport chain of isolated rat liver mitochondria
    S Krahenbuhl
    Department of Medicine, University of Berne, Switzerland
    Hepatology 19:471-9. 1994
    ..bile acids on the function of the electron transport chain was investigated in intact and disrupted rat liver mitochondria. In intact mitochondria, lipophilic bile acids used at a concentration of 100 mumol/L (0...
  17. ncbi BH3 death domain peptide induces cell type-selective mitochondrial outer membrane permeability
    B M Polster
    Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 276:37887-94. 2001
    ..Overall, results support the hypothesis that BH3 peptide releases cytochrome c by a Bax-dependent process that is independent of the mitochondrial permeability transition pore but regulated by Bcl-2...
  18. ncbi Electron transfer between cytochrome c and p66Shc generates reactive oxygen species that trigger mitochondrial apoptosis
    Marco Giorgio
    Experimental Oncology Department, European Institute of Oncology, Milan, Italy
    Cell 122:221-33. 2005
    ..These data demonstrate the existence of alternative redox reactions of the mitochondrial electron transfer chain, which evolved to generate proapoptotic ROS in response to specific stress signals...
  19. ncbi Mitochondrial ATP-sensitive K(+) channels as redox signals to liver mitochondria in response to hypertriglyceridemia
    Luciane C Alberici
    Departamento de Patologia Clinica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, 13083 887 Campinas, SP, Brazil
    Free Radic Biol Med 47:1432-9. 2009
    ..Isolated HTG liver mitochondria presented lower rates of H(2)O(2) production, which were reversed by mitoK(ATP) antagonists...
  20. pmc Activation of the lifespan regulator p66Shc through reversible disulfide bond formation
    Melanie Gertz
    Departments of Physiological Chemistry and Analytical Chemistry, Ruhr University Bochum, Universitatsstrasse 150, 44801 Bochum, Germany
    Proc Natl Acad Sci U S A 105:5705-9. 2008
    ..Glutathione and thioredoxins can reduce and inactivate p66(Shc), resulting in a thiol-based redox sensor system that initiates apoptosis once cellular protection systems cannot cope anymore with cellular stress...
  21. pmc Direct effects of diazoxide on mitochondria in pancreatic B-cells and on isolated liver mitochondria
    T Grimmsmann
    Institute of Pharmacology and Toxicology, University of Gottingen, Germany
    Br J Pharmacol 123:781-8. 1998
    ..Ca2+ transport, oxygen consumption and ATP generation were investigated in mouse pancreatic B-cells and rat liver mitochondria. 2...
  22. ncbi Acrolein is a mitochondrial toxin: effects on respiratory function and enzyme activities in isolated rat liver mitochondria
    Lijuan Sun
    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People s Republic China
    Mitochondrion 6:136-42. 2006
    ..However, the toxic effects and the underlying mechanisms of acrolein on mitochondria, especially, on liver mitochondria, have not been well studied...
  23. ncbi Alleviation of enhanced oxidative stress and oxygen consumption of L-thyroxine induced hyperthyroid rat liver mitochondria by vitamin E and curcumin
    U Subudhi
    Department of Biotechnology, Utkal University, Bhubaneswar 751004, Orissa, India
    Chem Biol Interact 173:105-14. 2008
    ....
  24. pmc Function of mitochondrial Stat3 in cellular respiration
    Joanna Wegrzyn
    Department of Biochemistry and Molecular Biology and Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298, USA
    Science 323:793-7. 2009
    ..These data indicate that Stat3 is required for optimal function of the ETC, which may allow it to orchestrate responses to cellular homeostasis...
  25. ncbi In vitro effect of eugenol and cinnamaldehyde on membrane potential and respiratory chain complexes in isolated rat liver mitochondria
    J Usta
    American University of Beirut, Department of Biochemistry, Faculty of Medicine, Lebanon
    Food Chem Toxicol 40:935-40. 2002
    ..The study proposes the mitochondria as a target for the action of the spices resulting in derangement of mitochondrial functions, particularly at proton transferring sites...
  26. ncbi Rat liver mitochondria can synthesize nicotinamide adenine dinucleotide from nicotinamide mononucleotide and ATP via a putative matrix nicotinamide mononucleotide adenylyltransferase
    M Barile
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Bari, Italy
    Biochem Mol Biol Int 38:297-306. 1996
    ..insight into the mechanism by which nicotinamide nucleotides localize in mitochondria, NMN was added to rat liver mitochondria, with NAD synthesis tested both enzymatically and by means of HPLC...
  27. ncbi Effect of experimental and cold exposure induced hyperthyroidism on H2O2 production and susceptibility to oxidative stress of rat liver mitochondria
    P Venditti
    Dipartimento delle Scienze Biologiche Sezione di Fisiologia, Universita di Napoli, I 80134 Napoli, Italy
    Arch Biochem Biophys 447:11-22. 2006
    ..However, a significant role is also played by T4, which appears to acts mainly modulating T3 effects, but also inducing some effects different from the T3 ones...
  28. pmc Alpha-tocopherol beta-oxidation localized to rat liver mitochondria
    Debbie J Mustacich
    Linus Pauling Institute, 571 Weniger Hall, Oregon State University, Corvallis, OR 97331, USA
    Free Radic Biol Med 48:73-81. 2010
    ..An unexpected finding was that alpha-CEHC, the end product of alpha-tocopherol metabolism, was found almost exclusively in mitochondria. These data are the first to indicate a mitochondrial role in alpha-tocopherol metabolism...
  29. ncbi Metallothionein can function as a chaperone for zinc uptake transport into prostate and liver mitochondria
    Leslie C Costello
    Department of Biomedical Sciences, Dental School, University of Maryland, 666 West Baltimore Street, Baltimore, MD 21201, USA
    J Inorg Biochem 98:664-6. 2004
    ..determined if Zn(7)-MT was an effective chaperone and donor for delivery and uptake of zinc by prostate and liver mitochondria. The results reveal that both intact mitochondria and mitoplasts effectively accumulated zinc from Zn(7)-MT...
  30. ncbi Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload
    G L Pardo Andreu
    Departamento de Investigaciones Biomédicas, Centro de Química Farmacéutica, Calle 200, esq 21, Playa, CP 11600, Ciudad de La Habana, Cuba
    Arch Toxicol 83:47-53. 2009
    ..Here we tested the effects of acute iron overload on liver mitochondria isolated from rats subjected to a single dose of i.p. 500 mg/kg iron-dextran...
  31. ncbi Complex I is the major site of mitochondrial superoxide production by paraquat
    Helena M Cocheme
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, United Kingdom
    J Biol Chem 283:1786-98. 2008
    ..Together this membrane potential-dependent uptake across the mitochondrial inner membrane and the subsequent rapid reduction to the paraquat radical cation explain the toxicity of paraquat to mitochondria...
  32. ncbi Modification of the mitochondrial proteome in response to the stress of ethanol-dependent hepatotoxicity
    Aparna Venkatraman
    Department of Pathology, University of Alabama, Birmingham, Alabama 35294, USA
    J Biol Chem 279:22092-101. 2004
    ..These data also suggest that the response of mitochondria to stress may not involve non-discriminate changes in the proteome but is restricted to those metabolic pathways that have a direct role in a specific pathology...
  33. ncbi Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria
    Xinhui Tang
    Institute of Materia Medica, School of Medicine and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China
    J Nutr Biochem 17:177-82. 2006
    ..It can be concluded that TCCE have protective activities against liver mitochondrial damage induced by CCl4, which suggests a new mechanism of the hepatoprotective effects of TCCE...
  34. ncbi The proton permeability of the inner membrane of liver mitochondria from ectothermic and endothermic vertebrates and from obese rats: correlations with standard metabolic rate and phospholipid fatty acid composition
    P S Brookes
    Department of Biochemistry, University of Cambridge, UK
    Comp Biochem Physiol B Biochem Mol Biol 119:325-34. 1998
    We measured the proton leak across the inner membrane of liver mitochondria isolated from six different vertebrate species and from obese and control Zucker rats...
  35. ncbi Inhibition of lipid peroxidation and protein oxidation in rat liver mitochondria by curcumin and its analogues
    Qing Yi Wei
    National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, Gansu 730, 000, China
    Biochim Biophys Acta 1760:70-7. 2006
    ..The in vitro oxidative damage to both lipids and proteins in rat liver mitochondria was used as a model to study the free radical-induced oxidative damage of biological lipids as well as ..
  36. pmc Closure of VDAC causes oxidative stress and accelerates the Ca(2+)-induced mitochondrial permeability transition in rat liver mitochondria
    Andrey Tikunov
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 27599, USA
    Arch Biochem Biophys 495:174-81. 2010
    ..Treatment of isolated rat liver mitochondria with 5microM G3139, an 18-mer phosphorothioate blocker of VDAC, accelerated onset of the MPT by 6.8+/-1...
  37. ncbi Effects of Phyllanthus urinaria extract on HepG2 cell viability and oxidative phosphorylation by isolated rat liver mitochondria
    Nuannoi Chudapongse
    School of Biology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand
    J Ethnopharmacol 130:315-9. 2010
    ..However, there is limited scientific evidence supporting its use in cancer particularly hepatocellular carcinoma...
  38. ncbi Mitochondrial oxidative injury and energy metabolism alteration in rat fatty liver: effect of the nutritional status
    G Vendemiale
    Department of Internal and Public Medicine (DIMIMP, University of Bari, Italy
    Hepatology 33:808-15. 2001
    ..These changes, which are greatly exacerbated after starvation, may account for the reduced synthesis of the hepatic ATP observed in the presence of fatty infiltration...
  39. pmc Inhibition of the mitochondrial permeability transition by protein kinase A in rat liver mitochondria and hepatocytes
    Peter Pediaditakis
    Center for Cell Death, Injury and Regeneration, Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, 29425, USA
    Biochem J 431:411-21. 2010
    ..combination with cyclic nucleotides to delay onset of the calcium-induced MPT was evaluated in isolated rat liver mitochondria. Liver cytosol plus cGMP or cAMP dose-dependently inhibited the MPT, required ATP hydrolysis for inhibition ..
  40. ncbi Bcl-xL promotes the open configuration of the voltage-dependent anion channel and metabolite passage through the outer mitochondrial membrane
    M G Vander Heiden
    Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 276:19414-9. 2001
    ..Together these results demonstrate that following an apoptotic signal, Bcl-x(L) can maintain metabolite exchange across the outer mitochondrial membrane by inhibiting VDAC closure...
  41. ncbi Heat shock suppresses the permeability transition in rat liver mitochondria
    Lihua He
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599 7090, USA
    J Biol Chem 278:16755-60. 2003
    ..These results indicate that heat shock causes resistance to opening of MPT pores, which may contribute to heat shock protection against cellular injury...
  42. pmc Crystal structures of medium-chain acyl-CoA dehydrogenase from pig liver mitochondria with and without substrate
    J J Kim
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226
    Proc Natl Acad Sci U S A 90:7523-7. 1993
    ..Lys-304, the prevalent mutation site found in patients with medium-chain acyl-CoA dehydrogenase deficiency, is located approximately 20 A away from the active site of the enzyme...
  43. pmc Age-dependent deficiency in import of mitochondrial DNA glycosylases required for repair of oxidatively damaged bases
    Bartosz Szczesny
    Sealy Center for Molecular Sciences, University of Texas Medical Branch, Galveston, TX 77555, USA
    Proc Natl Acad Sci U S A 100:10670-5. 2003
    ..These results indicate an age-dependent decline in the mitochondrial import of proteins needed for DNA repair and possibly for other functions...
  44. ncbi Metformin promotes isolated rat liver mitochondria impairment
    Cristina Carvalho
    Department of Zoology Faculty of Sciences and Technology, University of Coimbra, Coimbra 3000 354, Portugal
    Mol Cell Biochem 308:75-83. 2008
    ..In the present study, we evaluated the effect of metformin in isolated rat liver mitochondria status...
  45. ncbi Influence of aging and long-term caloric restriction on oxygen radical generation and oxidative DNA damage in rat liver mitochondria
    Monica Lopez-Torres
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, Madrid, Spain
    Free Radic Biol Med 32:882-9. 2002
    ..Long-term caloric restriction significantly decreased H2O2 production of rat liver mitochondria (47% reduction) and significantly reduced oxidative damage to mtDNA (46% reduction) with no changes in nDNA...
  46. ncbi Cyclosporin A-sensitive permeability transition pore is involved in Cd(2+)-induced dysfunction of isolated rat liver mitochondria: doubts no more
    Elena A Belyaeva
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr 44, 194223, St Petersburg, Russia
    Arch Biochem Biophys 405:252-64. 2002
    There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+)...
  47. ncbi p53 functions in the incorporation step in DNA base excision repair in mouse liver mitochondria
    Nadja C de Souza-Pinto
    Laboratory of Molecular Gerontology, NIA IRP, National Institutes of Health, Baltimore, MD 21224, USA
    Oncogene 23:6559-68. 2004
    ..Thus, we conclude that p53 may participate in mtBER by stimulating the repair synthesis incorporation step...
  48. pmc Bax directly induces release of cytochrome c from isolated mitochondria
    J M Jurgensmeier
    Program on Apoptosis and Cell Death Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 95:4997-5002. 1998
    ....
  49. ncbi Stimulation of respiration by methylene blue in rat liver mitochondria
    T M Visarius
    Department of Clinical Pharmacology, University of Berne, Switzerland
    FEBS Lett 412:157-60. 1997
    The effect of methylene blue on isolated rat liver mitochondria in the presence and absence of chloroacetaldehyde was investigated. Fatty acid oxidation was inhibited by chloroacetaldehyde and subsequently stimulated by methylene blue...
  50. ncbi Control of oxidative phosphorylation by Complex I in rat liver mitochondria: implications for aging
    Barbara Ventura
    Dipartimento di Biochimica G Moruzzi, Universita di Bologna, Via Irnerio 48, 40126, Bologna, Italy
    Biochim Biophys Acta 1553:249-60. 2002
    We compared NAD-dependent state 4 and state 3 respiration, NADH oxidation and Complex I specific activity in liver mitochondria from 4- and 30-month-old rats. All the activities examined were significantly decreased with aging...
  51. ncbi The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species
    H R McLennan
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia
    J Bioenerg Biomembr 32:153-62. 2000
    ..Complex II, in particular, appears to contribute to the basal production of ROS in cells...
  52. ncbi Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis
    Pierre Douette
    Laboratory of Bioenergetics, Bat B6c, Allee de la Chimie 3, 4000 Liege, Belgium
    J Proteome Res 4:2024-31. 2005
    ..Interestingly, major proteins that regulate generation and consumption of the acetyl-CoA pool were dramatically changed during steatosis. Many proteins involved in the response to oxidative stress were also affected...
  53. ncbi Dithiothreitol abrogates the effect of arsenic trioxide on normal rat liver mitochondria and human hepatocellular carcinoma cells
    Manash K Paul
    Department of Biology, Indian Institute of Science Education and Research, Mohali, MGSIPA Complex, Adjacent Sacred Heart School, Sector 26, Chandigarh 160019 corrected India
    Toxicol Appl Pharmacol 226:140-52. 2008
    ..Hence, we attempted to study the modulatory effect of DTT on ATO-induced dysfunction of mammalian liver mitochondria and human hepatocellular carcinoma cell line (Hep3B)...
  54. pmc Bax interacts with the permeability transition pore to induce permeability transition and cytochrome c release in isolated mitochondria
    M Narita
    Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 95:14681-6. 1998
    ..Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores...
  55. pmc Zinc metallothionein imported into liver mitochondria modulates respiration
    B Ye
    Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G Mudd Building, 250 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 98:2317-22. 2001
    Metallothionein (MT) localizes in the intermembrane space of liver mitochondria as well as in the cytosol and nucleus...
  56. ncbi Effect of melatonin treatment on oxygen consumption by rat liver mitochondria
    C F Reyes-Toso
    Departamento de Fisiologia and Hospital de Clinicas, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
    Amino Acids 31:299-302. 2006
    ..Basal state 4 respiration was not modified by melatonin. Melatonin, by curtailing overstimulation of cellular respiration caused by excess Krebs' cycle substrates, can protect the mitochondria from oxidative damage...
  57. ncbi Effect of short-term caloric restriction on H2O2 production and oxidative DNA damage in rat liver mitochondria and location of the free radical source
    R Gredilla
    Department of Animal Biology II (Animal Physiology, Faculty of Biology, Complutense University, Madrid, Spain
    J Bioenerg Biomembr 33:279-87. 2001
    ..complexes I or III of the respiratory chain contain the ROS generators, in this study we find that rat liver mitochondria generate oxygen radicals at complexes I, II, and III...
  58. ncbi Obesity induces expression of uncoupling protein-2 in hepatocytes and promotes liver ATP depletion
    K D Chavin
    Department of Surgery, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 274:5692-700. 1999
    ..Hence, hepatocytes adapt to obesity by up-regulating UCP2. However, because this decreases the efficiency of energy trapping, the cells become vulnerable to ATP depletion when energy needs increase acutely...
  59. ncbi Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases
    Bensu Karahalil
    Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 278:33701-7. 2003
    ..We did not detect a backup glycosylase or glycosylase/AP lyase activity for thymine glycol in the mitochondrial mouse extracts...
  60. ncbi Mechanism of primary Cd2+-induced rat liver mitochondria dysfunction: discrete modes of Cd2+ action on calcium and thiol-dependent domains
    Elena A Belyaeva
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr44, 194223, St Petersburg, Russia
    Toxicol Appl Pharmacol 192:56-68. 2003
    ..Suppositions about possible localization of separate sites of direct Cd2+ effects on mitochondrial function were made...
  61. ncbi Ethanol consumption and liver mitochondria function
    C C Cunningham
    Department of Biochemistry, Wake Forest University School of Medicine, Winston Salem, N C 27157 1016, USA
    Biol Signals Recept 10:271-82. 2001
    ..Both these semiquinones readily donate electrons to molecular oxygen to form superoxide...
  62. ncbi Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities
    A J Sanyal
    Division of Gastroenterology Hepatology, Department of Internal Medicine, Virginia Commonwealth University Medical College of Virginia, Richmond, Virginia 23298 0711, USA
    Gastroenterology 120:1183-92. 2001
    ....
  63. ncbi Fe(2+) induces a transient Ca(2+) release from rat liver mitochondria
    Vladimir Gogvadze
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Arch Biochem Biophys 398:198-202. 2002
    ..When Fe(2+) accumulation is completed, reuptake of released Ca(2+) into mitochondria resumes. Finally, we propose that Fe(2+) either inhibits Ca(2+) entry at the uniporter or is transported by it into the matrix...
  64. ncbi Effect of thyroid state on H2O2 production by rat liver mitochondria
    Paola Venditti
    Dipartimento di Fisiologia Generale ed Ambientale, Universita di Napoli, I 80134 Napoli, Italy
    Mol Cell Endocrinol 205:185-92. 2003
    ..order to throw light on this subject, the effects of thyroid state on O2 consumption and H2O2 release by rat liver mitochondria were investigated...
  65. ncbi Effect of ageing and caloric restriction on specific markers of protein oxidative damage and membrane peroxidizability in rat liver mitochondria
    Adrian J Lambert
    School of Biological Sciences, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK
    Mech Ageing Dev 125:529-38. 2004
    To gain insight into the anti-ageing mechanisms of caloric restriction (CR), liver mitochondria were isolated from male Brown-Norway rats of different ages (fully fed control and CR) and various specific markers of non-enzymatic protein ..
  66. ncbi Dietary choline restriction causes complex I dysfunction and increased H(2)O(2) generation in liver mitochondria
    K Hensley
    Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73014, USA
    Carcinogenesis 21:983-9. 2000
    ....
  67. pmc Metabolism of melatonin by cytochrome P450s in rat liver mitochondria and microsomes
    Igor Semak
    Department of Biochemistry, Belarus State University, Minsk, Belarus
    J Pineal Res 45:515-23. 2008
    ....
  68. ncbi Effects of a high fat diet on liver mitochondria: increased ATP-sensitive K+ channel activity and reactive oxygen species generation
    Ariel R Cardoso
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, Brazil
    J Bioenerg Biomembr 42:245-53. 2010
    ..the diet does not substantially alter respiratory rates, ADP/O ratios or membrane potentials of isolated liver mitochondria. However, H(2)O(2) release using different substrates and ATP-sensitive K(+) transport activities are ..
  69. ncbi Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C
    Mohamad Radwan Almofti
    Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1, Tokushima 770 8505
    J Biochem 134:43-9. 2003
    ..study, we examined the effect of Ag(+), a known cytotoxic sulfhydryl-reactive heavy metal, on isolated rat liver mitochondria. The following results were obtained: (1) Upon addition, Ag(+) instantly induced mitochondrial swelling and ..
  70. ncbi The anti-oxidant ebselen antagonizes the release of the apoptogenic factor cytochrome c induced by Fe2+/citrate in rat liver mitochondria
    A Boireau
    Departement Biologie, Aventis Pharma S A, Centre de Recherche de Vitry Alfortville, 13 quai Jules Guesde, 94403, Vitry sur Seine Cedex, France
    Neurosci Lett 289:95-8. 2000
    ..Thus, it is suggested that the neuroprotective effect of ebselen previously demonstrated in humans and in animals may be due, at least in part, to a mitochondrial protection...
  71. ncbi Defective hepatic mitochondrial respiratory chain in patients with nonalcoholic steatohepatitis
    Mercedes Pérez-Carreras
    Department of Gastroenterology, Hospital Universitario Doce de Octubre, Carretera de Andalucia, km 5, 400, 28041 Madrid, Spain
    Hepatology 38:999-1007. 2003
    ..In conclusion, activities of MRC complexes were decreased in liver tissue of patients with NASH. This dysfunction correlated with serum TNF-alpha, insulin resistance, and BMI values...
  72. ncbi Distinct kinetics of carnitine palmitoyltransferase i in contact sites and outer membranes of rat liver mitochondria
    F Fraser
    Cellular Biochemistry, Hannah Research Institute, Ayr, KA6 5HL, Scotland, United Kingdom
    J Biol Chem 276:20182-5. 2001
    Carnitine palmitoyltransferase I (CPT I) of rat liver mitochondria is an integral, polytopic protein of the outer membrane that is enriched at contact sites...
  73. ncbi Sulfide oxidation coupled to ATP synthesis in chicken liver mitochondria
    R Yong
    Biology Department, University of Massachusetts, Amherst, MA 01003, USA
    Comp Biochem Physiol B Biochem Mol Biol 129:129-37. 2001
    Chicken liver mitochondria consumed O2 at an accelerated rate when supplied with low concentrations of hydrogen sulfide...
  74. ncbi Cd2+ effects on respiration and swelling of rat liver mitochondria were modified by monovalent cations
    S M Korotkov
    Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Torez pr, Russian Federation
    J Inorg Biochem 70:17-23. 1998
    Changes in Cd2+ effects on respiration of succinate-energized rat liver mitochondria were studied after replacement of 100 mM KCl in an incubation medium by equimolar amounts of NaCl or LiCl, or by 200 mM sucrose. In KCl medium, 2...
  75. ncbi Rapid mtDNA deletion by oxidants in rat liver mitochondria after hemin exposure
    Hagir B Suliman
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Free Radic Biol Med 32:246-56. 2002
    ..This mtDNA damage is associated with altered expression of mitochondrial cell death proteins, thereby suggesting a novel mechanism for systemic or environmental pro-oxidants to influence apoptosis...
  76. ncbi Hypothyroidism reduces tricarboxylate carrier activity and expression in rat liver mitochondria by reducing nuclear transcription rate and splicing efficiency
    Luisa Siculella
    Laboratory of Biochemistry and Molecular Biology, Department of Biological and Environmental Science and Technologies, University of Lecce, Via provinciale Lecce Monteroni, I 73100 Lecce, Italy
    J Biol Chem 281:19072-80. 2006
    ..Thus, the rate of formation of the TCC 3'-end is not altered in hypothyroidism. These results suggest that hypothyroidism affects TCC expression at both the transcriptional and post-transcriptional levels...
  77. pmc Altered enzyme activities and citrulline synthesis in liver mitochondria from ornithine carbamoyltransferase-deficient sparse-furash mice
    N S Cohen
    Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033
    Biochem J 257:251-7. 1989
    ..A study of liver mitochondria from normal and spfash males showed that they differ in important ways...
  78. ncbi Modulation of oxidative phosphorylation machinery signifies a prime mode of anti-ageing mechanism of calorie restriction in male rat liver mitochondria
    Diksha Dani
    Physical Biochemistry, Department of Chemistry, Technische Universitat Darmstadt, Petersenstrasse 22, Darmstadt, Germany
    Biogerontology 11:321-34. 2010
    ..This is the first report on such 'putatively hormetic' ageing-analogous effects of CR, besides the age-retarding ones...
  79. ncbi Flow cytometric analysis of isolated liver mitochondria to detect changes relevant to cell death
    Gustav Mattiasson
    Laboratory for Experimental Brain Research, Wallenberg Neuroscience Center, Lund University, Lund, Sweden
    Cytometry A 60:145-54. 2004
    ..This article describes a well-characterized protocol for flow cytometric analysis of isolated liver mitochondria that can be used to detect mitochondrial alterations relevant to cell death.
  80. ncbi Protective effect of lipoic acid against methotrexate-induced oxidative stress in liver mitochondria
    Heena Tabassum
    Immunotoxicology Laboratory, Department of Medical Elementology and Toxicology, Jamia Hamdard Hamdard University, New Delhi 110062, India
    Food Chem Toxicol 48:1973-9. 2010
    ..The purpose of this study was to investigate the damage caused by MTX on liver mitochondria and its protection by using antioxidant properties of lipoic acid...
  81. ncbi Plasma lysine concentration and availability of 2-ketoglutarate in liver mitochondria
    P Kamoun
    Laboratoire de Biochimie Medicale B, Hĵpital Necker Enfants Malades, Paris, France
    J Inherit Metab Dis 25:1-6. 2002
    ..Our data further suggest that patients with isolated L-2-hydroxyglutaric aciduria may have a defect in 2-ketoglutarate metabolism. The current report summarizes our studies...
  82. ncbi Quantitative analysis of cereulide, an emetic toxin of Bacillus cereus, by using rat liver mitochondria
    Kumiko Kawamura-Sato
    Department of Medical Technology, Nagoya University Graduate School of Health Science, Nagoya, Aichi, Japan
    Microbiol Immunol 49:25-30. 2005
    ..The oxygen consumption in a reaction medium containing rat liver mitochondria was rapid in the presence of cereulide...
  83. ncbi Aminoacetone induces iron-mediated oxidative damage to isolated rat liver mitochondria
    Fernando Dutra
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Av Prof Lineu Prestes 748, SP, CEP 05508 900, Brazil
    Arch Biochem Biophys 430:284-9. 2004
    ..These findings may contribute to clarify the role of accumulated AA and iron overload in the mitochondrial oxidative damage reportedly occurring in diabetes mellitus...
  84. ncbi Adaptation of protein carbonyl detection to the requirements of proteome analysis demonstrated for hypoxia/reoxygenation in isolated rat liver mitochondria
    T Reinheckel
    Department of Experimental Surgery, University of Magdeburg, Magdeburg, D 39120, Germany
    Arch Biochem Biophys 376:59-65. 2000
    ..However, most protein spots did not show a change or even a decline (4 spots) in protein carbonyls...
  85. ncbi Multiple effects of 2,2',5,5'-tetrachlorobiphenyl on oxidative phosphorylation in rat liver mitochondria
    Vida Mildaziene
    Vytautas Magnus University, Vileikos 8, 3035 Kaunas, Lithuania
    Toxicol Sci 65:220-7. 2002
    ..pollutant 2,2',5,5'-tetrachlorobiphenyl (2,2',5,5'-TCB) was performed by modular kinetic analysis in rat liver mitochondria oxidizing succinate (+ rotenone) and glutamate + malate...
  86. pmc Absence of nitric-oxide synthase in sequentially purified rat liver mitochondria
    Priya Venkatakrishnan
    Department of Biological Sciences, University of Texas, El Paso, Texas 79968, USA
    J Biol Chem 284:19843-55. 2009
    ..repeated differential centrifugation followed by Percoll gradient fractionation, ultrapure, never frozen rat liver mitochondria and submitochondrial particles were obtained...
  87. ncbi Food restriction affects energy metabolism in rat liver mitochondria
    Jean Francois Dumas
    INSERM EMI U 00 18, Laboratoire de Biochimie et de Biologie Moleculaire, Medecine B, CHU, F 49033 Angers cedex 01, France
    Biochim Biophys Acta 1670:126-31. 2004
    To examine the effect of 50% food restriction over a period of 3 days on mitochondrial energy metabolism, liver mitochondria were isolated from ad libitum and food-restricted rats...
  88. pmc Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity
    S Shimizu
    Osaka University Graduate School of Medicine, Biomedical Research Center, Department of Medical Genetics, CREST of Japan Science and Technology Corporation JST, 2 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 97:577-82. 2000
    ..These findings indicated the involvement of a different mechanism in BH3-only, protein-induced apoptogenic cytochrome c release...
  89. ncbi DQAsome/DNA complexes release DNA upon contact with isolated mouse liver mitochondria
    V Weissig
    Department of Pharmaceutical Sciences, Bouve College of Health Sciences, Northeastern University, 211 Mugar Building, 360 Huntington Avenue, Boston, MA 02115, USA
    J Control Release 75:401-8. 2001
    ..Thus, DQAsome/DNA complexes were shown to release DNA upon contact with the surface of mitochondria thereby meeting a key requirement for our strategy towards mitochondrial DNA delivery...
  90. ncbi Methylmercury induces the opening of the permeability transition pore in rat liver mitochondria
    Marcantonio Bragadin
    Dipartimento di Scienze Ambientali, Universita di Venezia, DD2137 30123, Venice, Italy
    J Inorg Biochem 89:159-62. 2002
    ..This comparison suggests that CH(3)HgCl induces opening of a permeability transition pore (MTP). Since the opening of an MTP induces cell death, this interaction with MTP could be one of the causes of toxicity of CH(3)HgCl...
  91. ncbi Proton leak in hepatocytes and liver mitochondria from archosaurs (crocodiles) and allometric relationships for ectotherms
    A J Hulbert
    Metabolic Research Centre and Department of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia
    J Comp Physiol B 172:387-97. 2002
    ..Respiration rates of hepatocytes and liver mitochondria were measured at 37 degrees C and compared with values obtained for rat or previously measured for other ..
  92. ncbi Effect of poly(amido)amine (PAMAM) G4 dendrimer on heart and liver mitochondria in an animal model of diabetes
    Magdalena Labieniec
    Department of General Biophysics, University of Lodz, 12 16 Banacha Street, 90 237 Lodz, Poland
    Cell Biol Int 34:89-97. 2010
    ..5 micromol/kg of body weight daily for 60 days) may contribute to improved respiration in heart and liver mitochondria from streptozotocin-diabetic rats...
  93. ncbi Ca2+-induced reactive oxygen species production promotes cytochrome c release from rat liver mitochondria via mitochondrial permeability transition (MPT)-dependent and MPT-independent mechanisms: role of cardiolipin
    Giuseppe Petrosillo
    Department of Biochemistry and Molecular Biology and Consiglio Nazionale delle Ricerche Institute of Biomembranes and Bioenergetics, University of Bari, Via E Orabona, 4, 70126 Bari, Italy
    J Biol Chem 279:53103-8. 2004
    ..This study tested the hypothesis that distinct Ca(2+) thresholds may induce cytochrome c release from rat liver mitochondria by membrane permeability transition (MPT)-dependent and independent mechanisms...
  94. ncbi Rat brain and liver mitochondria develop oxidative stress and lose enzymatic activities on aging
    Ana Navarro
    Department of Biochemistry and Molecular Biology, University of Cadiz, Spain
    Am J Physiol Regul Integr Comp Physiol 287:R1244-9. 2004
    ..b>Liver mitochondria isolated from young and old rats did not show differences in fragility and water permeability...
  95. ncbi Structure, function and regulation of the tricarboxylate transport protein from rat liver mitochondria
    R S Kaplan
    Department of Pharmacology, College of Medicine, University of South Alabama, Mobile 36688
    J Bioenerg Biomembr 25:503-14. 1993
    ..Potential future research directions are discussed...
  96. pmc Proton conductance and fatty acyl composition of liver mitochondria correlates with body mass in birds
    Martin D Brand
    MRC Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK
    Biochem J 376:741-8. 2003
    The proton conductance of isolated liver mitochondria correlates significantly with body mass in mammals, but not in ectotherms...
  97. ncbi Protective effect of Phyllanthus fraternus against bromobenzene induced mitochondrial dysfunction in rat liver mitochondria
    Sriram Gopi
    Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India
    Food Chem Toxicol 48:2170-5. 2010
    ..extract of Phyllanthus fraternus (AEPF) against bromobenzene induced mitochondrial dysfunction in rat liver mitochondria. Administration of bromobenzene (10 mmol/kg body wt...
  98. ncbi Oxidative stress is responsible for mitochondrial permeability transition induction by salicylate in liver mitochondria
    Valentina Battaglia
    Dipartimento di Chimica Biologica, Universita di Padova, Istituto di Neuroscienze del C N R, Unità per lo studio delle Biomembrane, Viale G Colombo 3, 35121 Padova, Italy
    J Biol Chem 280:33864-72. 2005
    The interaction of salicylate with the respiratory chain of liver mitochondria generates hydrogen peroxide and, most probably, other reactive oxygen species, which in turn oxidize thiol groups and glutathione...
  99. ncbi Effects of acute leptin administration on the differences in proton leak rate in liver mitochondria from ob/ob mice compared to lean controls
    H P Melia
    Department of Biochemistry, Trinity College Dublin, Ireland
    FEBS Lett 458:261-4. 1999
    In this investigation, the effects on proton leak of leptin administration to ob/ob mice was measured for liver mitochondria. We and others have shown that proton leak is approximately 3 times greater in liver mitochondria from ob/ob mice ..
  100. ncbi The amino acid sequences of the flavin-peptides of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver mitochondria
    R J Cook
    J Biol Chem 260:12998-3002. 1985
    ..The sequence determined for the flavin-peptide from sarcosine dehydrogenase contained 14 amino acid residues Leu-Thr-Ser-Gly-Thr-Thr-Trp-His(flavin)-Thr-Ala-Gly-Leu-Gly-Arg...
  101. ncbi Steady-state redox behavior of cytochrome c, cytochrome a, and CuA of cytochrome c oxidase in intact rat liver mitochondria
    J E Morgan
    Department of Medical Chemistry, University of Helsinki, Finland
    Biochemistry 30:948-58. 1991
    ..of cytochrome c (Fec), Fea, and CuA of cytochrome c oxidase during steady-state turnover in intact rat liver mitochondria under coupled and uncoupled conditions...

Research Grants63

  1. Cell Volume Regulated Biology in the Liver
    Ravi Chari; Fiscal Year: 2005
    ..In specific aim 1, the pharmacological characteristics of these receptors in isolated rat liver mitochondria will be established...
  2. Role of Mitochondria in the Regulation of Iron metabolism by Alcohol
    DUYGU DEE DEE HARRISON-FINDIK; Fiscal Year: 2012
    ..The knowledge obtained from these studies, characterizing the pivotal role of mitochondria, will enable us to determine the mechanisms of liver injury caused by iron and alcohol in patients with ALD. ..
  3. Defining Molecular Determinants of Type 2 Diabetes Using Quantitative Proteomics
    Paul A Grimsrud; Fiscal Year: 2013
    ..I observed highly reproducible alterations in protein abundance and phosphorylation in liver mitochondria in a cohort of over forty mice, which are either susceptible (B6) or resistant (BTBR) to developing T2DM ..
  4. High-throughput assays for the development of SIRT5-specific inhibitors
    Hening Lin; Fiscal Year: 2012
    ..Furthermore, several malonylated and succinylated proteins were identified from bovine liver mitochondria, demonstrating that lysine malonylation and succinylation are previously unrecognized protein ..
  5. MITOCHONDRIAL CALCIUM SIGNALING IN CELL DEATH
    Gyorgy Hajnoczky; Fiscal Year: 2013
    ..studies, we have shown that hepatocarcinoma mitochondria are far more sensitive to Bid than normal liver mitochondria and this difference is paralleled by more VDAC2 and Bak being present in the hepatocarcinoma...
  6. Reactivating Apoptosis in Refractory Cancer by Selective Targeting of MCL-1
    Michelle Stewart; Fiscal Year: 2010
    ..mitochondrial apoptosis by SIMs will be examined in cytochrome c release assays performed on isolated mouse liver mitochondria. The ability of SIMs to reactivate apoptosis alone and in combination with other pro-apoptotic stimuli will ..
  7. TISSUE STORES OF FOLATE: DIETARY CONTROL AND ASSAY
    Conrad Wagner; Fiscal Year: 2013
    ..Two of these are enzymes that were found to be present in liver mitochondria, dimethylglycine dehydrogenase (DMGDH) and sarcosine dehydrogenase (SDH)...
  8. MECHANISM OF GLUTATHIONE CONJUGATE DEPENDENT TOXICITY
    Arthur Cooper; Fiscal Year: 2007
    ..cells in vivo, and that halogenated cysteine S-conjugates are metabolic poisons of isolated kidney and liver mitochondria. The PI and coworkers have suggested that KGDHC and BCKAD are sensitive to inactivation due to toxicant ..
  9. Mitochondrial SOD & Breast Cancer Risk-Mechanism
    JAMES YAGER; Fiscal Year: 2003
    ..The protein encoded by the Val allele exhibited decreased import in isolated rat liver mitochondria. Furthermore, Val homozygotes had an increased risk of nonfamilial cardiomyopathy in Japanese patients...
  10. CARBON DIOXIDE EXCHANGES IN THE LIVER
    SUSANNA DODGSON; Fiscal Year: 1991
    The aim of this proposal is to further study the nature of the unique liver mitochondria carbonic anhydrase, CAmit...
  11. BIOENERGETIC ASPECTS OF ALCOHOL CONSUMPTION
    William Thayer; Fiscal Year: 1991
    ..Isolated hepatocytes, liver mitochondria, phosphorylating submitochondrial particles, and phospholipid vesicles reconstituted with specific ..
  12. Molecular Regulation of Ureagenesis
    LJUBICA M CALDOVIC; Fiscal Year: 2010
    ..My hypothesis is that the ratio of two NAGS variants in the liver mitochondria is determined by the organism's nitrogen load...
  13. ALTERNATIVE ROLES FOR PHOSPHOENOLPYRUVATE CARBOXYKINASE
    Paul Ray; Fiscal Year: 1991
    ..of Mn2+ and Fe2+ depletion or repletion on OAA formation from PEP will be studied in isolated rabbit liver mitochondria. Conversion of glucose carbon to fatty acids will be studied in rabbit hepatocytes, white and brown ..
  14. REGULATORY MECHANISMS IN ENERGY METABOLISM
    Robert Harris; Fiscal Year: 1992
    ..on the above parameters; (b) the nature of the effects of acute glucagon treatment on energy transduction by liver mitochondria; (c) the nature of hypo- and hyperthyroidism on mitochondrial bioenergetics; (d) liver mitochondria from ..
  15. APPLICATION OF NOVEL OPTICAL METHODS TO CELL DYNAMICS
    Alan Verkman; Fiscal Year: 2001
    ..These experiments will be done in rat liver mitochondria, which possess, a 50 - 60 percent solids v/v, and in erythrocytes, whose solid concentration can be varied ..
  16. THE STATE OF WATER AND SOLUTES IN MITOCHONDRIA
    Keith Garlid; Fiscal Year: 1980
    Isolated rat liver mitochondria are being studied to determine the physiochemical properties of the internal aqueous phase...
  17. ANT Defects in Neurodegenerative Diseases
    Douglas Wallace; Fiscal Year: 2005
    ..We will also generate mice that are Ant1 and Ant2 null mutants in their livers and use the liver mitochondria to investigate the role of the ANTs in the mtPTP and in apoptosis...
  18. Zinc Regulation & Toxicity in Cellular Energy Metabolism
    Abraham Brown; Fiscal Year: 2006
    ..3) Establish the order of mitochondria events following exogenous addition of Zn++ in rat liver mitochondria. 4) Redox balance and other Zn++ -induced changes discovered and characterized in liver mitochondria in Aims ..
  19. CONTROL OF ENERGY METABOLISM IN CHRONIC ALCOHOLISM
    BORIS KHOLODENKO; Fiscal Year: 1999
    ..from heart and liver; (2) to quantify the ethanol-induced changes in the control patterns of heart and liver mitochondria under different conditions of substrate supply and energy utilization...
  20. ENZYME STEREOCHEMISTRY AND MECHANISM
    DONALD ABERHART; Fiscal Year: 1990
    Isovaleryl CoA dehydrogenase, an enzyme involved in leucine metabolism, will be isolated from rat liver mitochondria and used to transform stereospecifically labeled (3S)-[4-13C]-isovaleryl CoA to Beta-methylcrotonyl CoA...
  21. Mitochondrial GSH /SAM in alcohol induced liver damage
    JOSE FERNANDEZ CHECA; Fiscal Year: 2004
    ..1. - SAM levels and transport in rat liver mitochondria from alcohol-fed rats...
  22. TRNA PROCESSING NUCLEASES FROM LIVER MITOCHONDRIA
    GLENN VAN TUYLE; Fiscal Year: 1990
    The 5' and 3' tRNA processing nucleases from liver mitochondria will be further purified using current methods of enzyme purification...
  23. THE TOTAL SYNTHESIS OF SARRACENIN
    JAMES WHITESELL; Fiscal Year: 1980
    ..interesting properties, the most important being a pronounced ability to inhibit respiration in rat liver mitochondria. The former may be a by-product of a potent antileukemic substance present in sarracenin flavia, the natural ..
  24. CHRONIC ALCOHOLISM & LIVER ENERGY & LIPID METABOLISM
    Carol Cunningham; Fiscal Year: 1992
    ..Our previous studies have documented several ethanol-induced lesions in liver mitochondria. The intact hepatocyte will now be investigated to determine if the depression in mitochondrial function ..
  25. MITOCHONDRIAL CATION TRANSPORT MECHANISMS
    JOYCE DIWAN; Fiscal Year: 1991
    ..Physiological measurements have indicated that the mechanisms which mediate trans[port of K+ and Mg++ into liver mitochondria are similar in their substrate dependence, pH dependence, and sensitivity to inhibitory and stimulatory ..
  26. PKG-dependent inhibition of the MPT
    Peter Pediaditakis; Fiscal Year: 2006
    ..To achieve this aim, MPT assays with isolated rat liver mitochondria incubated with liver cytosol will be employed to assess the involvement of PKG and PKA pathways in ..
  27. ENZYME DEFECTS IN DISORDERS OF PROTOPORPHYRIN METABOLISM
    Joseph Bloomer; Fiscal Year: 2005
    ..b>Liver mitochondria will be examined for the presence of heterodimers consisting of the normal and mutant FECH proteins...
  28. Technologies Trageting Mitochondral Proteome
    BRIAN BALGLEY; Fiscal Year: 2005
    ..proteolytic digestion of proteins resolved and eluted from the CGE-based separations enables the combined top-down/bottom-up characterization of PTMs in mouse liver mitochondria as the model system in this study.
  29. MITOCHONDRIAL PYRUVATE AND TRICARBOXYLATE TRANSPORTERS
    RONALD KAPLAN; Fiscal Year: 1991
    ..for 5 years to study the pyruvate/H+ symporter and the tricarboxylate-H+/dicarboxylate antiporter of rate liver mitochondria. These systems will be studied with respect to structure and function at the molecular/chemical levels, and ..
  30. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANT ELEMENTS
    ROBERT GHOLSON; Fiscal Year: 1980
    The origin of intramitochondrial pyridine nucleotides will be studied by 1) incubating rat liver mitochondria with NAD ion labeled with 14C in the adenine moiety and with 3H in the nicotinamide reisolating and washing the mitochondria and ..
  31. REGULATION OF HEPATIC FATTY ACID OXIDATION
    GEORGE COOK; Fiscal Year: 1992
    ..in vivo and in vitro using normal rats, isolated rat hepatocytes, cultured hepatocytes, isolated rat liver mitochondria isolated mitochondrial outer membranes, and isolated, purified carnitive palmitoyltransferase...
  32. Mitochondrial Role in Manganese Toxicity
    Thomas Gunter; Fiscal Year: 2010
    ....
  33. OXIDANT HEPATIC INJURY IN CHOLESTASIS
    Ronald Sokol; Fiscal Year: 2005
    ....
  34. Rare Liver Disease Network
    Ronald Sokol; Fiscal Year: 2005
    ..The RLDN will be a full partner in the Rare Diseases Clinical Research Network and will participate collaboratively with the other Clinical Research Centers and the Data and Technology Coordinating Center. ..
  35. ANTIVIRAL & ANTIFIBROTIC LIVER THERAPY OF HCV+ DRINKERS
    Charles Lieber; Fiscal Year: 2005
    ..Funds are requested for special laboratory tests, study nurses, travel to meetings, patient monitoring expenses and a core office. ..
  36. Redox Modification of Thiols in Alcohol Hepatotoxicity
    Shannon Bailey; Fiscal Year: 2008
    ..New information on the molecular targets of SAM will also be achieved, which will enable the design of effective therapeutic strategies to treat liver diseases. [unreadable] [unreadable] [unreadable] [unreadable]..
  37. NOVEL THERAPIES FOR ACETAMINOPHEN TOXICITY
    Laura James; Fiscal Year: 2004
    ..abstract_text> ..
  38. Improving Survival For Small-For-Size Liver Grafts
    Zhi Zhong; Fiscal Year: 2004
    ..This work will develop mechanism-based strategies to increase the use and improve the outcome of partial livers for transplantation in the clinic. ..
  39. MODULATION OF NEUROENDOCRINE FUNCTION IN SEPSIS
    DREW CARLSON; Fiscal Year: 2005
    ..The mechanisms revealed are likely to play a significant role in the suppression of host defense and the onset of septic shock...
  40. Glutathione in Environmental Toxicity and Disease
    HOWARD SHERTZER; Fiscal Year: 2007
    ..Our proposed research should therefore help focus future genotype-phenotype association studies between the appropriate DNA variant sites in the GCLC and GCLM genes and disease. ..
  41. Induction of Inflammation by Mitochondrial Proteins
    Elliott Crouser; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  42. Evaluation of a Biomarker for Acetaminophen Toxicity
    Laura James; Fiscal Year: 2005
    ..The data generated from this study will lead to enhanced recognition of APAP hepatotoxicity, thus allowing for better identification of "at risk" populations and the creation of prevention strategies. ..
  43. RECEPTOR MEDIATED OXIDATIVE STRESS
    HOWARD SHERTZER; Fiscal Year: 2003
    ..These studies will improve our understanding of how PHAHs such as TCDD generate oxidative stress and mutagenesis, leading to better models for risk assessment and novel strategies for intervention in PHAH-mediated toxicity. ..
  44. The impact of growth modulation of liver carcinogenesis
    Xiao Ming Yin; Fiscal Year: 2009
    ....
  45. High-Content Cell-Based Screening for Modulators of Autophagy
    Xiao Ming Yin; Fiscal Year: 2007
    ..Our long term hope is that the knowledge and the materials developed through this study will be valuable for future clinical applications. [unreadable] [unreadable] [unreadable]..
  46. GLUTATHIONE AND MITOCHONDRIA IN TOXIC RENAL INJURY
    Lawrence Lash; Fiscal Year: 2003
    ..This information may have therapeutic applications for prevention of renal cellular injury or for understanding mitochondrial diseases or age-related decreases that occur in mitochondrial function. ..
  47. Stromal Gene Expression During Pulmonary Sarcoidosis
    Elliott Crouser; Fiscal Year: 2005
    ..Statistical comparisons of gene expression patterns will then be performed. Novel insights relating gene profiles to disease manifestation, prognosis and pathogenesis are likely outcomes of these investigations. ..
  48. Nitric oxide and mitochondrial biogenesis in sepsis
    Claude A Piantadosi; Fiscal Year: 2010
    ..abstract_text> ..
  49. Proteomics of Oxidant-Induced Mitochondrial Damage in an Animal Model of NASH
    Shannon Bailey; Fiscal Year: 2007
    ..transgenic and/or knockout mouse models to investigate mechanisms of protection from metabolic stress in liver mitochondria. The second aim is to characterize post-translational modifications to the hepatic mitochondria proteome in ..
  50. MOLECULAR TOXICOLOGY IN HUMAN KIDNEY CELLS
    Lawrence Lash; Fiscal Year: 2007
    ..Achievement of these aims should build on our previous work in human kidney cells and extend it to provide a much more complete understanding of the various and complex ways in which DCVC affects the human kidney [unreadable] [unreadable]..
  51. Synergistic Nutraceutical Effects of DLPC and SAMe
    Charles Lieber; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  52. CHRONIC ALCOHOLISM AND LIVER ENERGY AND LIPID METABOLISM
    Carol Cunningham; Fiscal Year: 2004
    ..Phosphorus NMR spectroscopy will be utilized heavily in these latter studies. ..
  53. DLPC Treatment of Alcoholic and Non-Alcoholic Fibrosis
    Charles Lieber; Fiscal Year: 2006
    ..abstract_text> ..
  54. Bc1-2 Family Proteins in the Ischemic Neuronal Injury
    Xiao Ming Yin; Fiscal Year: 2006
    ..To characterize the molecular interactions of Bid and Bax in inducing dysfunction of mitochondria isolated from the brain. ..
  55. PROTON LEAK, OXIDATIVE STRESS, AND ENERGY RESTRICTION
    Jon Ramsey; Fiscal Year: 2003
    ..The premise is that Caloric Restriction (CR), hypothyroidism, and modulation of dietary fat will decrease mitochondrial permeability (i.e. proton leak), therefore decreasing molecular Oxygen consumption, and presumably oxidative stress. ..
  56. Chronic Alcohol and Liver Oxidative Stress
    Shannon Bailey; Fiscal Year: 2003
    ..This investigation should thus provide information on ethanol-elicited alterations that contribute to oxidative damage in the liver. ..
  57. MECHANISM AND ROLE OF MICROSOMAL ETHANOL OXIDATION
    Charles Lieber; Fiscal Year: 2001
    ..Such information is aimed at elucidating key aspects of alcohol mediated pathology in a way which may eventually affect alcohol related medical complications, their prevention and treatment. ..
  58. MITOCHONDRIAL CYTOCHROME C RELEASE VIA MEMBRANE PORES
    Elliott Crouser; Fiscal Year: 2004
    ..These advisors are actively investigating the role mitochondria in the pathogenesis of disease. Thus, the prospects for successful collaborative initiatives, a critical determinant of future success, are excellent. ..
  59. Ethanol effects on liver in self-administering primates
    Carol Cunningham; Fiscal Year: 2004
    ..fascicularis will develop liver pathology past the fatty liver stage and 2) to evaluate the efficacy of using blood and urine samples to follow development of alcoholic liver disease in an animal model. ..
  60. Lung Injury Protection by Coagulation Blockade
    CLAUDE PIANTADOSI; Fiscal Year: 2008
    ..A successfully optimized strategy could greatly attenuate persistence of pulmonary inflammation and facilitate the resolution of human ARDS. ..
  61. Antiviral/Antifibrotic Liver Therapy of Hepatitis C positive Alcohol Drinkers
    Charles Lieber; Fiscal Year: 2007
    ..Accordingly, support is requested for the completion of this novel approach for the treatment of HCV+ patients who become abstinent or who continue to consume alcohol moderately. [unreadable] [unreadable]..
  62. Liver Fibrosis, Inflammation & Oxidative Stress Markers
    Charles Lieber; Fiscal Year: 2006
    ..abstract_text> ..
  63. Mechanisms of In Vivo Ethanol-Induced Mitochondrial Depolarization
    Zhi Zhong; Fiscal Year: 2010
    ....