subcellular fractions


Summary: Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)

Top Publications

  1. ncbi Prediction of protein cellular attributes using pseudo-amino acid composition
    K C Chou
    Computer Aided Drug Discovery, Pharmacia, Kalamazoo, Michigan 49007 4940, USA
    Proteins 43:246-55. 2001
  2. pmc Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization
    Kuo Chen Chou
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, Shanghai, China
    PLoS ONE 5:e11335. 2010
  3. pmc A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila
    X Morin
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    Proc Natl Acad Sci U S A 98:15050-5. 2001
  4. ncbi PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization
    K Nakai
    Institute for Molecular and Cellular Biology, Osaka University, Japan
    Trends Biochem Sci 24:34-6. 1999
  5. pmc A multi-label classifier for predicting the subcellular localization of gram-negative bacterial proteins with both single and multiple sites
    Xuan Xiao
    Computer Department, Jing De Zhen Ceramic Institute, Jing De Zhen, China
    PLoS ONE 6:e20592. 2011
  6. pmc Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
    T Matsui
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    EMBO J 15:2208-16. 1996
  7. ncbi Toward a confocal subcellular atlas of the human proteome
    Laurent Barbe
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:499-508. 2008
  8. ncbi S3-12, Adipophilin, and TIP47 package lipid in adipocytes
    Nathan E Wolins
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:19146-55. 2005
  9. pmc Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes
    Kulandaivelu S Vetrivel
    Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, Illinois 60637, USA
    J Biol Chem 279:44945-54. 2004
  10. ncbi A top-down approach to enhance the power of predicting human protein subcellular localization: Hum-mPLoc 2.0
    Hong Bin Shen
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China
    Anal Biochem 394:269-74. 2009

Research Grants

Detail Information

Publications292 found, 100 shown here

  1. ncbi Prediction of protein cellular attributes using pseudo-amino acid composition
    K C Chou
    Computer Aided Drug Discovery, Pharmacia, Kalamazoo, Michigan 49007 4940, USA
    Proteins 43:246-55. 2001
  2. pmc Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization
    Kuo Chen Chou
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, Shanghai, China
    PLoS ONE 5:e11335. 2010
    ..It is anticipated that the Plant-mPLoc predictor as presented in this paper will become a very useful tool in plant science as well as all the relevant areas...
  3. pmc A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila
    X Morin
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    Proc Natl Acad Sci U S A 98:15050-5. 2001
    ..GFP reveals in real time the dynamics of protein's distribution in the whole, live organism and provides useful markers for a number of cellular structures and compartments...
  4. ncbi PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization
    K Nakai
    Institute for Molecular and Cellular Biology, Osaka University, Japan
    Trends Biochem Sci 24:34-6. 1999
  5. pmc A multi-label classifier for predicting the subcellular localization of gram-negative bacterial proteins with both single and multiple sites
    Xuan Xiao
    Computer Department, Jing De Zhen Ceramic Institute, Jing De Zhen, China
    PLoS ONE 6:e20592. 2011
    ..It is anticipated that iLoc-Gneg may become a useful high throughput tool for Molecular Cell Biology, Proteomics, System Biology, and Drug Development...
  6. pmc Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho
    T Matsui
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    EMBO J 15:2208-16. 1996
    ..Thus it is likely that Rho-kinase is a putative target serine/threonine kinase for Rho and serves as a mediator of the Rho-dependent signaling pathway...
  7. ncbi Toward a confocal subcellular atlas of the human proteome
    Laurent Barbe
    Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, SE 106 91 Stockholm, Sweden
    Mol Cell Proteomics 7:499-508. 2008
    ..The results suggest that this approach might be a valuable tool in conjunction with predictive models for protein localization...
  8. ncbi S3-12, Adipophilin, and TIP47 package lipid in adipocytes
    Nathan E Wolins
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:19146-55. 2005
  9. pmc Association of gamma-secretase with lipid rafts in post-Golgi and endosome membranes
    Kulandaivelu S Vetrivel
    Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, Illinois 60637, USA
    J Biol Chem 279:44945-54. 2004
    ..These findings are consistent with the localization of gamma-secretase in lipid raft microdomains of post-Golgi and endosomes, organelles previously implicated in amyloidogenic processing of APP...
  10. ncbi A top-down approach to enhance the power of predicting human protein subcellular localization: Hum-mPLoc 2.0
    Hong Bin Shen
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China
    Anal Biochem 394:269-74. 2009
    ..As a consequence, the prediction power has been significantly enhanced. The web server of Hum-mPLoc2.0 is freely accessible at
  11. ncbi A novel Usher protein network at the periciliary reloading point between molecular transport machineries in vertebrate photoreceptor cells
    Tina Maerker
    Department of Cell and Matrix Biology, Institute of Zoology, Johannes Gutenberg University of Mainz, 55099 Mainz, Germany
    Hum Mol Genet 17:71-86. 2008
    ..Since this structural specialization in amphibian photoreceptor cells defines a specialized membrane domain for docking and fusion of transport vesicles, we suggest a prominent role of the USH proteins in cargo shipment...
  12. ncbi Extensive feature detection of N-terminal protein sorting signals
    Hideo Bannai
    Human Genome Center, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, 108 8639, Japan
    Bioinformatics 18:298-305. 2002
    ..We adopt an approach which consists of an extensive search for simple rules and various attributes which is partially guided by human intuition...
  13. ncbi Prediction of subcellular localization using sequence-biased recurrent networks
    Mikael Boden
    School of Information Technology and Electrical Engineering, The University of Queensland, QLD 4072, Australia
    Bioinformatics 21:2279-86. 2005
    ..However, due to the low similarity of such sequences and the dynamical nature of the sorting process, the computational prediction of subcellular localization of proteins is challenging...
  14. pmc The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast
    D Otsuga
    Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA
    J Cell Biol 143:333-49. 1998
    ..Our results demonstrate that Dnm1p is required for the cortical distribution of the mitochondrial network in yeast, a novel function for a dynamin-related protein...
  15. ncbi Phospholipase D2, a distinct phospholipase D isoform with novel regulatory properties that provokes cytoskeletal reorganization
    W C Colley
    Program in Genetics, State University of New York, Stony Brook, New York 11794 8651, USA
    Curr Biol 7:191-201. 1997
    ..Biochemical and cell biological studies suggest, however, that additional and distinct PLD activities exist in cells, so a search was carried out for novel mammalian genes related to PLD1...
  16. ncbi Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization
    Holger Lorenz
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 18T Library Drive, Bethesda, Maryland 20892, USA
    Nat Methods 3:205-10. 2006
    ..The success of the FPP assay in characterizing the topology of the tested proteins within their appropriate compartments suggests this technique has wide applicability in studying protein topology and localization within the cell...
  17. pmc TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis
    Xinguo Mao
    The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China
    J Exp Bot 63:2933-46. 2012
    ..Therefore, TaNAC2 has potential for utilization in transgenic breeding to improve abiotic stress tolerances in crops...
  18. pmc Cell wall antibiotics provoke accumulation of anchored mCherry in the cross wall of Staphylococcus aureus
    Wenqi Yu
    Microbial Genetics, University of Tubingen, Tubingen, Germany
    PLoS ONE 7:e30076. 2012
    ..The effect is most likely due to antibiotic mediated increase of free anchoring sites (Lipid II) at the cross wall, the substrate of SrtA, leading to a preferential incorporation of anchored proteins at the cross wall...
  19. ncbi Cellular and subcellular change evoked by diffuse traumatic brain injury: a complex web of change extending far beyond focal damage
    Orsolya Farkas
    Department of Anatomy and Neurobiology, Medical College of Virginia Campus, Virginia Commonwealth University, P O Box 980709, Richmond, VA 23298, USA
    Prog Brain Res 161:43-59. 2007
    ..This review further illustrates that our once simplistic views framed by evidence based upon contusional and/or ischemic change do not fully explain the complex repertoire of change evoked by diffuse traumatic brain injury...
  20. pmc Regulation of anterograde transport of adrenergic and angiotensin II receptors by Rab2 and Rab6 GTPases
    Chunmin Dong
    Department of Pharmacology and Experimental Therapeutics, Louisiana State University, Health Sciences Center, New Orleans, LA 70112, USA
    Cell Signal 19:2388-99. 2007
    ..These data also provide the first evidence indicating that Rab6-coordinated retrograde transport selectively modulates intracellular trafficking and signaling of GPCRs...
  21. pmc Dynamic trafficking of wheat γ-gliadin and of its structural domains in tobacco cells, studied with fluorescent protein fusions
    Mathilde Francin-Allami
    UR1268 Biopolymeres Interactions Assemblages, INRA, BP71627 Nantes cedex 03, France
    J Exp Bot 62:4507-20. 2011
    ..Taken together, the results show that tobacco cells are a convenient model to study the atypical wheat prolamin trafficking with fluorescent protein fusions...
  22. ncbi Mouse very long-chain Acyl-CoA synthetase 3/fatty acid transport protein 3 catalyzes fatty acid activation but not fatty acid transport in MA-10 cells
    Zhengtong Pei
    Kennedy Krieger Research Institute and Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Biol Chem 279:54454-62. 2004
    ..ACSVL3 was found primarily in mitochondria of MA-10 and Neuro2a cells by both Western blot analysis of subcellular fractions and immunofluorescence analysis...
  23. ncbi MultiLoc: prediction of protein subcellular localization using N-terminal targeting sequences, sequence motifs and amino acid composition
    Annette Höglund
    Division for Simulation of Biological Systems, WSI ZBIT, Eberhard Karls University Tübingen Sand 14, D 72076 Tubingen, Germany
    Bioinformatics 22:1158-65. 2006
    ..An important step is taken towards emulating the protein sorting process by capturing and bringing together biologically relevant information, and addressing the clear need to improve prediction accuracy and localization coverage...
  24. pmc TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis
    Xinguo Mao
    The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    J Exp Bot 61:683-96. 2010
    ..4 could be utilized in transgenic breeding to improve abiotic stresses in crops...
  25. ncbi Predicting multisite protein subcellular locations: progress and challenges
    Pufeng Du
    School of Computer Science and Technology, Tianjin University, Tianjin 300072, China
    Expert Rev Proteomics 10:227-37. 2013
    ..Finally, a vision of the future of predicting multisite protein subcellular locations is given...
  26. ncbi Subcellular localization and cytoplasmic complex status of endogenous Keap1
    Yoriko Watai
    Graduate School of Comprehensive Human Sciences, Center for Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Japan
    Genes Cells 12:1163-78. 2007
    ..These results demonstrate that endogenous Keap1 remains mostly in the cytoplasm, and electrophiles promote nuclear accumulation of Nrf2 without altering the subcellular localization of Keap1...
  27. ncbi Nuclear-cytoplasmic shuttling of APC regulates beta-catenin subcellular localization and turnover
    B R Henderson
    Westmead Institute for Cancer Research, University of Sydney, Westmead Millenium Institute, Westmead NSW 2145, Australia
    Nat Cell Biol 2:653-60. 2000
    ..These findings suggest that wild-type APC controls the nuclear accumulation of beta-catenin by a combination of nuclear export and cytoplasmic degradation...
  28. ncbi K+ channel activity in plants: genes, regulations and functions
    Anne Lebaudy
    Biochimie et Physiologie Moleculaire des Plantes, UMR 5004, CNRS INRA Monptellier SupAgro UM2, 1 Place Viala, 34060 Montpellier Cedex 1, France
    FEBS Lett 581:2357-66. 2007
  29. pmc Leucine-rich repeat protein PRAME: expression, potential functions and clinical implications for leukaemia
    Frances Wadelin
    Gene Regulation Group, Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, Nottingham, UK
    Mol Cancer 9:226. 2010
    ..Here we review the current knowledge of the structure/function of PRAME and its relevance in leukaemia...
  30. ncbi Regulated intramembrane proteolysis of Bri2 (Itm2b) by ADAM10 and SPPL2a/SPPL2b
    Lucas Martin
    Center for Integrated Protein Science Munich and Adolf Butenandt Institute, Department of Biochemistry, Laboratory for Neurodegenerative Disease Research, Ludwig Maximilians University, 80336 Munich, Germany
    J Biol Chem 283:1644-52. 2008
  31. ncbi Virus-mPLoc: a fusion classifier for viral protein subcellular location prediction by incorporating multiple sites
    Hong Bin Shen
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, China
    J Biomol Struct Dyn 28:175-86. 2010
    ..For reader's convenience, a user-friendly web-server for Virus-mPLoc was designed that is freely accessible at
  32. ncbi Fumarase: a paradigm of dual targeting and dual localized functions
    Ohad Yogev
    Department of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, Hebrew University, Jerusalem, Israel
    FEBS J 278:4230-42. 2011
    ..Strikingly, upon DNA damage the protein is transported from the cytosol to the nucleus, where by virtue of its enzymatic activity it participates in the DNA damage response...
  33. pmc Low density subcellular fractions enhance disease-specific prion protein misfolding
    James F Graham
    Neuropathogenesis Division, The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Alexander Robertson Building, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK
    J Biol Chem 285:9868-80. 2010
    ..We also show that subcellular fractions enhancing disease-specific prion protein conversion prevent in vitro fibrillization of recombinant prion ..
  34. ncbi Global implications of mRNA localization pathways in cellular organization
    Eric Lecuyer
    Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario, Canada M5S 3E1
    Curr Opin Cell Biol 21:409-15. 2009
    ..This review focuses on these recent functional genomic approaches, their implications, and the new tools and methods that will be needed to further elucidate mRNA localization pathways...
  35. ncbi Non-nuclear Wld(S) determines its neuroprotective efficacy for axons and synapses in vivo
    Bogdan Beirowski
    Laboratory of Molecular Signalling, The Babraham Institute, Cambridge, UK
    J Neurosci 29:653-68. 2009
    ..We conclude that Wld(S) can act in one or more non-nuclear compartments to protect axons and synapses, and that molecular changes can enhance its therapeutic potential...
  36. pmc Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity
    Feng Qian Li
    Department of Pharmacological Sciences and 2Graduate Program in Genetics, State University of New York at Stony Brook, Stony Brook, NY 11794, USA
    J Cell Biol 181:1141-54. 2008
    ..Our results therefore suggest a novel paradigm through which Cby acts in concert with 14-3-3 proteins to facilitate nuclear export of beta-catenin, thereby antagonizing beta-catenin signaling...
  37. pmc Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum
    Brook Pyhtila
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 14:445-53. 2008
    ..Combined, these data indicate that the mRNA localization to the ER can be conferred independent of the signal sequence/SRP pathway and suggest that mRNA localization to the ER may utilize cis-encoded targeting information...
  38. pmc pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis
    R R Adams
    Cancer Research Campaign CRC Laboratories, Cell Cycle Genetics Research Group, Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK
    Genes Dev 12:1483-94. 1998
    ..We suggest that PAV-KLP is required both to establish the structure of the telophase spindle to provide a framework for the assembly of the contractile ring, and to mobilize mitotic regulator proteins...
  39. ncbi Predicting subcellular localization of proteins in a hybridization space
    Yu Dong Cai
    Biomolecular Sciences Department, UMIST, PO Box 88, Manchester M60 1QD, UK
    Bioinformatics 20:1151-6. 2004
  40. ncbi Isoprenylation of RhoB is necessary for its degradation. A novel determinant in the complex regulation of RhoB expression by the mevalonate pathway
    Konstantinos Stamatakis
    Departamento de Estructura y Función de Proteínas, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas C S I C, Velazquez, 144, 28006 Madrid, Spain
    J Biol Chem 277:49389-96. 2002
    ..These results show that RhoB isoprenylation is necessary for rapid turnover of this protein and identify a novel link between the cholesterol biosynthetic pathway and the regulation of G-protein expression...
  41. pmc Combining machine learning and homology-based approaches to accurately predict subcellular localization in Arabidopsis
    Rakesh Kaundal
    Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA
    Plant Physiol 154:36-54. 2010
    ..A supplemental Web site that hosts all the training/testing data sets and whole proteome predictions is available at
  42. ncbi Processing, subcellular localization, and function of 519 (granulysin), a human late T cell activation molecule with homology to small, lytic, granule proteins
    S V Pena
    Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA
    J Immunol 158:2680-8. 1997
    ..Its lytic activity suggests the involvement of 519 in CTL effector function...
  43. pmc Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis
    Yang ja Lee
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 15:5001-11. 2004
    ..However, we provide further evidence that multiple components of the mitochondrial morphogenesis machinery can positively and negatively regulate apoptosis...
  44. ncbi Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation
    Ramachandra K Reddy
    Department of Biochemistry and Molecular Biology and the USC Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California 90089 9176, USA
    J Biol Chem 278:20915-24. 2003
    ..Lastly, a GRP78 mutant deleted of its ATP binding domain fails to bind procaspase-7 and loses its protective effect against etoposide-induced apoptosis...
  45. pmc The post-synaptic density of human postmortem brain tissues: an experimental study paradigm for neuropsychiatric illnesses
    Chang Gyu Hahn
    Department of Psychiatry, University of Pennsylvania, Philadelphia, PA, USA
    PLoS ONE 4:e5251. 2009
    ..This approach may foster novel postmortem brain research paradigms in which the stoichiometry and protein composition of specific microdomains are examined...
  46. ncbi Presenilin-1 is located in rat mitochondria
    Maria Ankarcrona
    Karolinska Institutet, Neurotec, KASPAC, Novum, 5th Floor, S 141 57 Huddinge, Sweden
    Biochem Biophys Res Commun 295:766-70. 2002
    ..For these studies we used tissue sections and subcellular fractions of rat brain and liver...
  47. ncbi The 'ins' and 'outs' of flavonoid transport
    Jian Zhao
    Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA
    Trends Plant Sci 15:72-80. 2010
    ..An improved understanding of transport mechanisms will facilitate the successful metabolic engineering of flavonoids for plant protection and human health...
  48. ncbi Subcellular compartmentalization of glutathione: correlations with parameters of oxidative stress related to genotoxicity
    Richard M Green
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Mutagenesis 21:383-90. 2006
    ..The findings highlight the critical roles of the mitochondrial and nuclear GSH pools in protecting cellular components, particularly DNA, from oxidative modification...
  49. ncbi PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis
    J L Gardy
    Department of Molecular Biology and Biochemistry, Simon Fraser University Burnaby, BC, Canada V5A 1S6
    Bioinformatics 21:617-23. 2005
    ..The goals of the present work are as follows: increase PSORTb's coverage while maintaining the existing precision level, expand it to include Gram-positive bacteria and then carry out a comparative analysis of localization...
  50. ncbi BubR1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte
    Liang Wei
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing, China
    Cell Cycle 9:1112-21. 2010
    ..Our data suggest that BubR1 is a spindle assembly checkpoint protein regulating meiotic progression of oocytes...
  51. ncbi Argonaute proteins: mediators of RNA silencing
    Lasse Peters
    Laboratory of RNA Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 26:611-23. 2007
    ..This review focuses on the various cellular functions of mammalian Argonaute proteins in conjunction with the different small RNA species that are known today...
  52. ncbi Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting
    Jaewon Ko
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Neurosci 23:1667-77. 2003
    ..These results suggest that the GIT1-liprin-alpha interaction is required for AMPA receptor targeting and that GIT1 may play an important role in the organization of presynaptic and postsynaptic multiprotein complexes...
  53. ncbi Insig-dependent ubiquitination and degradation of mammalian 3-hydroxy-3-methylglutaryl-CoA reductase stimulated by sterols and geranylgeraniol
    Navdar Sever
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9046, USA
    J Biol Chem 278:52479-90. 2003
  54. ncbi Structure of type IIbeta phosphatidylinositol phosphate kinase: a protein kinase fold flattened for interfacial phosphorylation
    V D Rao
    Laboratory of Molecular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0580, USA
    Cell 94:829-39. 1998
    ..A modeled complex with membrane-bound substrate and ATP shows how a phosphoinositide kinase can phosphorylate its substrate in situ at the membrane interface...
  55. pmc Mapping the Arabidopsis organelle proteome
    Tom P J Dunkley
    Department of Biochemistry, University of Cambridge, Downing Site, Cambridge CB2 1QW, United Kingdom
    Proc Natl Acad Sci U S A 103:6518-23. 2006
    ..Consequently, genuine organelle residents have been distinguished from contaminating proteins and proteins in transit through the secretory pathway...
  56. ncbi Better prediction of protein cellular localization sites with the k nearest neighbors classifier
    P Horton
    Computer Science Division, University of California, Berkeley 94720, USA
    Proc Int Conf Intell Syst Mol Biol 5:147-52. 1997
    ..The result is an accuracy of approximately 60% for 10 yeast classes and 86% for 8 E. coli classes. The best previously reported accuracies for these datasets were 55% and 81% respectively...
  57. ncbi Phosphorylation regulates intracellular trafficking of beta-secretase
    J Walter
    Adolf Butenandt Institute, Department of Biochemistry, Laboratory for Alzheimer s and Parkinson s Disease Research, Ludwig Maximilians University, 44 Schillerstrasse, 80336 Munich, Germany
    J Biol Chem 276:14634-41. 2001
    ..In contrast, nonphosphorylatable BACE S498A is retained within early endosomes. Our results therefore demonstrate regulated trafficking of BACE within the secretory and endocytic pathway...
  58. ncbi Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER
    Duncan T Browman
    Southern Alberta Cancer Research Institute, Departments of Oncology and Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, T2N 4N1, Canada
    J Cell Sci 119:3149-60. 2006
    ..Interestingly, the ER contains relatively low levels of cholesterol and sphingolipids compared with other organelles. Thus, our data support the existence of lipid-raft-like domains within the membranes of the ER...
  59. ncbi BaCelLo: a balanced subcellular localization predictor
    Andrea Pierleoni
    Biocomputing Group, Dept of Biology University of Bologna, Via Irnerio 42, 40126 Bologna, Italy
    Bioinformatics 22:e408-16. 2006
    ..Computational procedures are then needed for annotating the subcellular location of proteins in large scale genomic projects...
  60. pmc Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA
    S Mili
    Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029-6574, USA
    Mol Cell Biol 21:7307-19. 2001
    ..The characteristics of these complexes indicate that they result from RNP remodeling associated with mRNA maturation and delineate specific changes in RNP protein composition during formation and transport of mRNA in vivo...
  61. ncbi Nrf2 controls constitutive and inducible expression of ARE-driven genes through a dynamic pathway involving nucleocytoplasmic shuttling by Keap1
    Truyen Nguyen
    Schering Plough Research Institute, Kenilworth, New Jersey 07033, USA
    J Biol Chem 280:32485-92. 2005
  62. pmc Synaptic targeting of N-type calcium channels in hippocampal neurons
    Anton Maximov
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9040, USA
    J Neurosci 22:6939-52. 2002
    ..Our results provide a novel insight into the molecular mechanisms responsible for targeting of Ca2+ channels and other synaptic proteins in neurons...
  63. ncbi Regulation of hdm2 by stress-induced hdm2alt1 in tumor and nontumorigenic cell lines correlating with p53 stability
    Chrisanne S Dias
    Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA
    Cancer Res 66:9467-73. 2006
    ..Our data imply that HDM2(ALT1) possesses a normal physiologic function in damaged cells, perhaps facilitating cellular defense...
  64. ncbi Vanadium distribution, lipid peroxidation and oxidative stress markers upon decavanadate in vivo administration
    S S Soares
    CCMAR, Universidade do Algarve, Campus de Gambelas, 8005 139 Faro, Portugal
    J Inorg Biochem 101:80-8. 2007
  65. pmc Trafficking of Sendai virus nucleocapsids is mediated by intracellular vesicles
    Raychel Chambers
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA
    PLoS ONE 5:e10994. 2010
    ..Although viral ribonuclocapsid (vRNP) is an essential component of infectious virions, the process of vRNP translocation to assembly sites is poorly understood...
  66. pmc Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis
    Dietmar Funck
    Department of Plant Physiology and Biochemistry, Biology Section, University of Konstanz, Universitatsstrasse 10, 78464 Konstanz, Germany
    BMC Plant Biol 10:70. 2010
    ..While P5CDH is a single copy gene in Arabidopsis, two ProDH genes have been identified in the genome. Until now, only ProDH1 (At3g30775) had been functionally characterised...
  67. pmc TDP-43 and FUS/TLS: emerging roles in RNA processing and neurodegeneration
    Clotilde Lagier-Tourenne
    Department of Cellular and Molecular Medicine, University of California, La Jolla, CA 92093 6070, USA
    Hum Mol Genet 19:R46-64. 2010
    ..Their association with ALS and other neurodegenerative diseases is redirecting research efforts toward understanding the role of RNA processing regulation in neurodegeneration...
  68. ncbi Acetylation regulates subcellular localization of eukaryotic translation initiation factor 5A (eIF5A)
    Muhammad Ishfaq
    Chemical Genetic Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan
    FEBS Lett 586:3236-41. 2012
    ..As eIF5A is constitutively hypusinated under physiological conditions, we suggest that reversible acetylation plays a major role in controlling the subcellular localization of eIF5A...
  69. ncbi Construction of functional interaction networks through consensus localization predictions of the human proteome
    Solip Park
    School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790 784, Korea
    J Proteome Res 8:3367-76. 2009
    ..Thus, the use of consensus localization prediction, such as ConLoc, is a new approach for the identification of novel disease associated genes...
  70. pmc Deletions within its subcellular targeting domain enhance the axon protective capacity of Nmnat2 in vivo
    Stefan Milde
    Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom
    Sci Rep 3:2567. 2013
    ..Together, our results establish Nmnat2 localisation and turnover as a valuable target for modulating axon degeneration in vivo. ..
  71. pmc Transposition of a reconstructed Harbinger element in human cells and functional homology with two transposon-derived cellular genes
    Ludivine Sinzelle
    Max Delbruck Center for Molecular Medicine, 13092 Berlin, Germany
    Proc Natl Acad Sci U S A 105:4715-20. 2008
    ..The Harbinger transposon will serve as a useful experimental system for transposon biology and for investigating the enzymatic functions of domesticated, transposon-derived cellular genes...
  72. ncbi Activation of wild type p53 function by its mortalin-binding, cytoplasmically localizing carboxyl terminus peptides
    Sunil C Kaul
    Gene Function Research Center, National Institute of Advanced Industrial Science and Technology AIST, 1 1 1 Higashi, Tsukuba Science City 305 8562, Japan
    J Biol Chem 280:39373-9. 2005
    ..Such activation of p53 function was sufficient to cause growth arrest of human osteosarcoma and breast carcinoma cells...
  73. ncbi Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity
    R J Gonzalez
    Department of Pharmaceutical Sciences, University of the Sciences in Philadelphia, 600 South 43rd Street, PA 19104-4495, Philadelphia, USA
    Toxicol In Vitro 15:257-9. 2001
    ..Therefore, caution should be exercised in ascribing decreases in viability as due solely to mitochondrial damage when using either of these dyes...
  74. ncbi A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function
    N E Faulkner
    Department of Cell Biology University of Massachusetts Medical School, 377 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Cell Biol 2:784-91. 2000
    ..We conclude that LIS1 participates in a subset of dynein functions, and may regulate the division of neuronal progenitor cells in the developing brain...
  75. ncbi Multitask learning for protein subcellular location prediction
    Qian Xu
    Bioengineering Program, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong
    IEEE/ACM Trans Comput Biol Bioinform 8:748-59. 2011
    ..However, if they are closely related biologically, the multitask learning can do much better than individual learning...
  76. ncbi Differential distribution of harmonin isoforms and their possible role in Usher-1 protein complexes in mammalian photoreceptor cells
    Jan Reiners
    Institute for Zoology, Johannes Gutenberg University, Mainz, Germany
    Invest Ophthalmol Vis Sci 44:5006-15. 2003
    ..The purpose of the present study was to analyze the molecular and cellular functions of these USH1 proteins in the mammalian retina...
  77. pmc TESTLoc: protein subcellular localization prediction from EST data
    Yao Qing Shen
    Robert Cedergren Center for Bioinformatics and Genomics Biochemistry Department, Universite de Montreal, 2900 Edouard Montpetit, Montreal, QC, H3T 1J4, Canada
    BMC Bioinformatics 11:563. 2010
  78. ncbi Protein palmitoylation and subcellular trafficking
    Clara Aicart-Ramos
    Departamento de Bioquímica y Biología Molecular 1, Universidad Complutense, Madrid, Spain
    Biochim Biophys Acta 1808:2981-94. 2011
    ..In this article we will give an overview of how protein palmitoylation regulates protein trafficking and subcellular localization...
  79. ncbi SUMOylation attenuates the aggregation propensity and cellular toxicity of the polyglutamine expanded ataxin-7
    Alexandre Janer
    INSERM, UMRS 975, CNRS UMR 7225, Paris, France
    Hum Mol Genet 19:181-95. 2010
    ..Our results demonstrate an influence of SUMOylation on the multistep aggregation process of ATXN7 and implicate a role for ATXN7 SUMOylation in SCA7 pathogenesis...
  80. ncbi HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases
    Y Suzuki
    Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    J Immunol 158:2736-44. 1997
    ..Deletion mutant analysis of these proteins reveals that the association is mediated by the amino terminal region of HS1 and the carboxyl-terminal half of HAX-1. The potential role of the HAX-1/HS1 complex is discussed...
  81. pmc Intracellular localization and conformational state of transglutaminase 2: implications for cell death
    Soner Gundemir
    Department of Pharmacology and Physiology, University of Rochester, Rochester, NY, USA
    PLoS ONE 4:e6123. 2009
    ..In addition, nuclear localization of TG2 generally plays a key role in its protective function against cell death processes, either counteracting the detrimental effect or strengthening the protective role of the protein...
  82. ncbi Mitochondrial localization of the Parkinson's disease related protein DJ-1: implications for pathogenesis
    Li Zhang
    Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Hum Mol Genet 14:2063-73. 2005
    ..Our findings of DJ-1's mitochondrial localization may have important implications for understanding the pathogenesis of PD...
  83. ncbi CE-PLoc: an ensemble classifier for predicting protein subcellular locations by fusing different modes of pseudo amino acid composition
    Asifullah Khan
    Department of Information and Computer Sciences, Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan
    Comput Biol Chem 35:218-29. 2011
    ..47 and 83.99% are obtained for 12 and 14 subcellular locations datasets, respectively. In case of independent dataset test, prediction accuracies are 87.04 and 87.33% for 12 and 14 class datasets, respectively...
  84. ncbi Caldendrin, a novel neuronal calcium-binding protein confined to the somato-dendritic compartment
    C I Seidenbecher
    AG Molecular and Cellular Neurobiology, Institute for Medical Psychology, Otto von Guericke University, 39120 Magdeburg, Germany
    J Biol Chem 273:21324-31. 1998
    ..Therefore, this novel Ca2+-binding protein was named caldendrin...
  85. pmc Nucleocytoplasmic shuttling of BZR1 mediated by phosphorylation is essential in Arabidopsis brassinosteroid signaling
    Hojin Ryu
    Department of Life Sciences, Pohang University of Science and Technology, Pohang, Korea
    Plant Cell 19:2749-62. 2007
    ..We propose that the spatial redistribution of BZR1 is critical for proper BR signaling in plant growth and development...
  86. ncbi Predicting subcellular localization of gram-negative bacterial proteins by linear dimensionality reduction method
    Tong Wang
    Institute of Image Processing and Pattern Recognition, Shanghai Jiaotong University, Shanghai, 200240, China
    Protein Pept Lett 17:32-7. 2010
  87. ncbi Efficiency of von Willebrand factor-mediated targeting of interleukin-8 into Weibel-Palade bodies
    R Bierings
    Department of Plasma Proteins, Sanquin Research and Landsteiner Laboratory AMC, University of Amsterdam, Plesmanlaan 125, Amsterdam, The Netherlands
    J Thromb Haemost 5:2512-9. 2007
    ..After de novo synthesis in endothelial cells, the chemokine interleukin-8 (IL-8) is targeted to endothelial cell-specific storage vesicles, the Weibel-Palade bodies (WPBs), where it colocalizes with von Willebrand factor (VWF)...
  88. pmc Subnuclear proteomics in colorectal cancer: identification of proteins enriched in the nuclear matrix fraction and regulation in adenoma to carcinoma progression
    Jakob Albrethsen
    OncoProteomics Laboratory, Department of Medical Oncology, VU University Medical Center VUmc Cancer Center Amsterdam, The Netherlands
    Mol Cell Proteomics 9:988-1005. 2010
    ..Together, the results show that subnuclear proteomics of tumor tissue is feasible and a promising avenue for exploring oncogenesis...
  89. ncbi Predicting protein subcellular locations with feature selection and analysis
    Yudong Cai
    State Key Laboratory of Medical Genomics, Ruijin Hospital, Shanghai Jiao Tong University, 197 Rui Jin Road II, Shanghai 200025, China
    Protein Pept Lett 17:464-72. 2010, which may play a important complementary role to a series of web-server predictors summarized recently in a review by Chou and Shen (Chou, K.C., Shen, H.B. Natural Science, 2009, 2, 63-92,
  90. pmc Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells
    Samuel B Stephens
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:623-32. 2008
    ..These findings identify a role for the ER in global protein synthesis, and they suggest models where compartmentalization of the tRNA acylation/deacylation cycle contributes to the regulation of global protein synthesis rates...
  91. ncbi Glucose sensing through the Hxk2-dependent signalling pathway
    F Moreno
    Departamento de Bioquimica y Biologia Molecular, Universidad de Oviedo, 33006 Oviedo, Spain
    Biochem Soc Trans 33:265-8. 2005
    ..Our findings show that the main role of Hxk2 in the glucose signalling pathway is the interaction with Mig1 to generate a repressor complex located in the nucleus of S. cerevisiae...
  92. ncbi Characterization of subcellular fractions and distribution profiles of transport components involved in Ca(2+) homeostasis in rat vas deferens
    Christianne B V Scaramello
    Departamento de Farmacologia Basica e Clinica, Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Rio de Janeiro 21941 590, Brazil
    J Pharmacol Toxicol Methods 47:93-8. 2002
  93. pmc Regulation of cytochrome c oxidase activity by c-Src in osteoclasts
    Tsuyoshi Miyazaki
    Department of Cell Biology, Yale University School of Medicine, PO Box 208044, New Haven, CT 06520 8044, USA
    J Cell Biol 160:709-18. 2003
    ..These results suggest that c-Src plays a previously unrecognized role in maintaining cellular energy stores by activating Cox in mitochondria...
  94. ncbi Paxillin-beta-catenin interactions are involved in Rac/Cdc42-mediated endothelial barrier-protective response to oxidized phospholipids
    Anna A Birukova
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    Am J Physiol Lung Cell Mol Physiol 293:L199-211. 2007
    ..In turn, paxillin knockdown by specific siRNA attenuated AJ enhancement in response to OxPAPC. These results show for the first time the novel interactions between FA and AJ protein complexes critical for EC barrier regulation by OxPAPC...
  95. pmc In vivo imaging of alpha-synuclein in mouse cortex demonstrates stable expression and differential subcellular compartment mobility
    Vivek K Unni
    Alzheimer s Research Unit, MassGeneral Institute for Neurodegenerative Disease, MGH Harvard Medical School, Charlestown, Massachusetts, United States of America
    PLoS ONE 5:e10589. 2010
    ..We now develop methods to measure alpha-synuclein levels in the living mammalian brain to study in vivo protein mobility, turnover and degradation with subcellular specificity...
  96. ncbi Characterization of a novel wheat NAC transcription factor gene involved in defense response against stripe rust pathogen infection and abiotic stresses
    Ning Xia
    Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Plant Protection, Northwest A and F University, 712100, Yangling, Shaanxi, China
    Mol Biol Rep 37:3703-12. 2010
    ..These results indicate that this novel TaNAC4 gene functions as a transcriptional activator involved in wheat response to biotic and abiotic stresses...
  97. ncbi RanBPM is a phosphoprotein that associates with the plasma membrane and interacts with the integrin LFA-1
    Simona Denti
    Laboratory of Immunoregulation, Department of Immunology, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, France
    J Biol Chem 279:13027-34. 2004
    ..We suggest that RanBPM may constitute a molecular scaffold that contributes to coupling LFA-1 and other integrins with intracellular signaling pathways...
  98. ncbi Proteomic screening of salt-stress-induced changes in plasma membranes of Synechocystis sp. strain PCC 6803
    Fang Huang
    Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden
    Proteomics 6:910-20. 2006
    ..The physiological significance of the identified salt-stress proteins in the plasma membrane is discussed integrating our current knowledge on cyanobacterial stress physiology...
  99. pmc Analysis of the histone acetyltransferase B complex of maize embryos
    A Lusser
    Department of Microbiology, University of Innsbruck, Medical School, Fritz Pregl Strasse 3, A 6020 Innsbruck, Austria
    Nucleic Acids Res 27:4427-35. 1999
    ..Regardless of its predominant cytoplasmic localization, a significant proportion of HAT-B-p50 is present in nuclei, irrespective of the cell cycle stage, suggesting an additional nuclear function...
  100. pmc Systematic analysis of Arabidopsis organelles and a protein localization database for facilitating fluorescent tagging of full-length Arabidopsis proteins
    Shijun Li
    Department of Plant Biology, Carnegie Institution, Stanford, California 94305, USA
    Plant Physiol 141:527-39. 2006
    ..The database can be searched, browsed, and downloaded using a Web browser at The software can also be downloaded from the same Web site for local installation...
  101. ncbi Subcellular localization of beta-arrestins is determined by their intact N domain and the nuclear export signal at the C terminus
    Ping Wang
    Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, People s Republic of China
    J Biol Chem 278:11648-53. 2003

Research Grants62

  1. Dissecting the role of one neuronal RhoGEF amongst many: the Kalirin-7 null mouse
    Richard E Mains; Fiscal Year: 2012
    ..of pre- and post-synaptic elements, electrophysiological recordings of slices and biochemical analysis of subcellular fractions. Spine formation in hippocampal neurons prepared from Kal7KO mice can be rescued by expressing exogenous ..
  2. ClC-3 ion transport modulates NADPH oxidase-dependent PMN priming by endotoxin
    Jessica G Moreland; Fiscal Year: 2012
    ..To explore these aims biochemical analyses of PMN subcellular fractions in combination with neutrophil functional assays including measurement of NADPH oxidase activity, ..
  3. Sex-specific fetal programming of adult vascular dysfunction and hypertension
    ..We will examine the PKC isoenzyme expression profile in subcellular fractions, its phosphorylation status, and functional activity and examine mechanisms by which androgens regulate ..
    MICHAEL JOHN MACDONALD; Fiscal Year: 2013
    ..Recent work has shown the beta cell is a lipogenic tissue. Lipids in cells and subcellular fractions are measured with gas chromatography...
  5. Medications Development for the Pregnant Opiate Addict
    Mahmoud S Ahmed; Fiscal Year: 2010
    ..specific aims will be investigated utilizing the technique of dual perfusion of placental lobule and subcellular fractions trophoblast tissue. 1...
  6. Molecular Phenotyping of NOD Mice
    Ivan C Gerling; Fiscal Year: 2010
    ..subsets in NOD, NOR, and C57BL/6 female mice between 2 & 4 weeks of age (looking at whole cells and subcellular fractions with a variety of proteome characterization approaches). Specific Aim 3...
  7. Analysis of Polyhydroxyalkanoate Inclusion Biogenesis
    Douglas Dennis; Fiscal Year: 2009
    ..prove periplasmic localization of PhaP via fluorescence localization and Western blot analyses of subcellular fractions, 2) demonstrate that the inclusion envelope is derived from the cytoplasmic membrane by proteomic analysis,..
    Frank G Oppenheim; Fiscal Year: 2012
    ..For this purpose protein preparations of subcellular fractions of C. albicans which had been exposed to histatin 5 will be compared to controls over time...
  9. Carcinogenic Metabolites Formed from Antiestrogen
    Judy L Bolton; Fiscal Year: 2013
    ..The rate of formation, type of quinoid, as well as their reactivity will be studied in subcellular fractions and Ishikawa endometrial cells. 2...
  10. Landscape of transcription in human and mouse
    THOMAS RAYMOND GINGERAS; Fiscal Year: 2013
    ..for a subset of primary cell lines we will generate the above libraries from nuclear and cytoplasmic subcellular fractions. Long RNA-Seq data will be distilled down into functional elements consisting of splice junctions, ..
  11. 10th International Catecholamine Symposium (XICS)
    DANIEL T O'CONNOR; Fiscal Year: 2012
    ..Since catecholamine research formats span from subcellular fractions to cells and neurons to animal models and patients, research on this small chemical family is truly ..
  12. Characterization of zinc dysregulation in normal and cancerous prostate cells.
    JOSE GUADALUPE MIRANDA; Fiscal Year: 2012
    ..will be complemented by x-ray fluorescence microscopy on fixed cells to map total Zn+2 and ICP-MS on subcellular fractions. PUBLIC HEALTH RELEVANCE: Live cell imaging of Zn+2 ions in noncancerous and cancerous prostate cells will ..
    Ronald Rubin; Fiscal Year: 1991
    ..32P-IP3 binding to a specific cellular receptor site will be characterized in permeabilized cells and subcellular fractions. The concentration dependence of 1,4,5-IP3-induced 45Ca release will also be probed to delineate ..
    MARGARET GARNER; Fiscal Year: 2001
    ..The primary focus of the proposed research is to characterize the Na,K-ATPases of the subcellular fractions of normal lenses. Hypothesis I...
  15. Regulation of ER to Golgi Transport by Luminal Calcium
    Jesse C Hay; Fiscal Year: 2013
    ..ER/Golgi transport in intact mammalian cell lines and in vitro reconstitution of transport phenomena from subcellular fractions to dissect the roles of luminal Ca2+, the Ca2+ sensor ALG-2 and the COPII coat in specific stages of this ..
    Yvonne Lange; Fiscal Year: 1990 cultured human fibroblasts that several sterol intermediates are nonuniformly distributed among subcellular fractions. This has lead to the hypothesis that cholesterol biosynthesis is not taken to completion at a single ..
  17. Role of Red Cell Membrane in Malaria Parasite Release
    Manjit Hanspal; Fiscal Year: 2006 vivo? This will be achieved by immunodetection of FP-2 within parasite- infected erythrocytes, and in subcellular fractions, and by studying biosynthesis and maturation of FP-2...
    Brian Liu; Fiscal Year: 1993
    ..bound cathepsin B at a biochemical level using enzyme overlay membranes and isoelectric focusing of subcellular fractions. Furthermore, we will determine the effects of proteinase inhibitors in inhibiting the degradation of ..
    DIANE REIBEL; Fiscal Year: 1990
    ..The content and fatty acyl composition of the major phospholipids will be determined in these isolated subcellular fractions and membrane structure (fluidity) will be evaluated using electron spin resonance spectroscopy...
    Frederick Wilson; Fiscal Year: 1990
    ..The second aim is to isolate bile acid transporter proteins. For these studies, enriched subcellular fractions are solubilized and subjected to protein purification using a glycocholate derivative - Sepharose- 4B ..
    Anjaneyulu Kowluru; Fiscal Year: 2004
    ..e. subcellular fractions, intact islets, and purified proteins in reconstituted systems)...
    Richard Mitchell; Fiscal Year: 1999
    ..Analysis will include novel assays of antigen processing and presentation using subcellular fractions, allowing identification of intracellular sites of antigen degradation vs sites of MHC II association. 3...
  23. Role of CIC-3 channels in regulation of cell volume and shape in neutrophils
    Jessica Moreland; Fiscal Year: 2007
    ..We will explore these aims with a combination of basic physiology techniques, biochemical analyses of PMN subcellular fractions, and neutrophil functional assays...
    KATHLEEN MCCOY; Fiscal Year: 1993
    ..inside receptor positive APCs will be determined by the location of radiolabeled conjugates over time in subcellular fractions isolated by Percoll density centrifugation and compared with free transferrin and alpha 2- macroglobulin...
    FREDRIC KRAEMER; Fiscal Year: 1993
    ..LDL receptor content in subcellular fractions isolated by differential centrifugation will be quantitated using immunoblotting with specific anti-LDL ..
    MUNIR MOBASSALEN; Fiscal Year: 1993
    ..and distribution of Gb3 in proximal and distal small intestine, in crypt and villus cells and in subcellular fractions, will be studied at various ages...
    Gregory Mueller; Fiscal Year: 2003
    ..Cell-free models permit the direct investigation of fundamental reaction components. Subcellular fractions of N18TG2 mouse neuroblastoma cells, a line which expresses PAM and synthesizes oleamide, will be used to ..
    James Crawford; Fiscal Year: 1993
    ..isolated perfused liver, hepatocytes and sinusoidal cells, and determination of the relative affinity of subcellular fractions of the liver for each probe...
    John Amatruda; Fiscal Year: 1992
    ..hypothesis that the insulin receptor kinase is under intracellular regulation and is not identical in all subcellular fractions. Methodologies to be employed included primary cultures of rat hepatocytes, subcellular fractionation of ..
    Mark Koury; Fiscal Year: 1993
    ..EP-induced changes in synthesis and accumulation of non-globin proteins will be determined in several subcellular fractions of the erythroblasts...
    Robert Lehrer; Fiscal Year: 1991
    ..constitutive antifungal components (Con-AFC) of normal peripheral blood mononuclear cells by screening subcellular fractions of mononuclear cells for activity against Candida albicans and Cryptococcus neoformans in vitro...
    Carl Weiner; Fiscal Year: 2005
    ..Photoaffinity labeling with a nonhydrolyzable, biotinylated substrate is used in subcellular fractions of arteries obtained from pregnant, nonpregnant, and estrogen or progesterone-treated castrate animals ..
    Ann Hubbard; Fiscal Year: 1990
    ..ATP-dependent pH changes of the fluorescein derivative in enriched subcellular fractions will be measured...
    Bruce Ames; Fiscal Year: 2006
    ..Mature RBCs from healthy human donors will be analyzed in whole and subcellular fractions. Proteins will be separated in liquid phase by isoelectric point and molecular weight and then identified ..
    Dennis Ahnen; Fiscal Year: 1990
    ..The location of the solubilizing enzyme will be determined by incubating subcellular fractions in the standard assay to detect solubilization...
    RONALD JANIS; Fiscal Year: 1980 to determine whether there are differences in Ca ions regulation or related enzymes between subcellular fractions from mesenteric arteries of Spontaneously Hypertensive Rats (SHR) and those fractions from normotensive ..
    RUSSELL DELUDE; Fiscal Year: 2007
    ..barrier function and TJ formation at levels of integration ranging from whole animals to cultured cells or subcellular fractions. In vitro, we will focus on changes in intestinal epithelial permeability using Caco-2 (human enterocyte-..
    FREDRICK GEORGE; Fiscal Year: 1992
    ..5 alpha- reductase and aromatase activities will be assessed in tissue slices and in subcellular fractions of the gubernaculum throughout the period of testicular descent...
    SIENYAO CHOW; Fiscal Year: 1992 is that the purpose of regulating carbonic anhydrase is to preserve the activity of the enyzme in subcellular fractions where it is essential for the normal functions performed by the cells...
    Theodore Gabig; Fiscal Year: 1993
    ..proposal are: (1) to further define the sequential requirements for growth factors, membrane and cytosolic subcellular fractions for initiation of DNA replication; (2) to produce a panel of inhibitory monoclonal antibodies to cytosolic ..
  41. Regulation of Celluar Zinc Distribution
    Wolfgang Maret; Fiscal Year: 2004
    ..Employing a new differential fluorescence labeling procedure, MT/T ratios will be determined in subcellular fractions. Then, the translocation of MT and T from the cytosol to the mitochondrion, the nucleus, and the ..
    Nicola Longo; Fiscal Year: 1999
    ..and biotin labeling of ion transporters on the plasma membrane of intact cells, Western blot analysis on subcellular fractions (using antibodies specific for each transporter and anti- phosphotyrosine antibodies), immunoprecipitation ..
    Leonard Jarett; Fiscal Year: 1980
    ..These studies will use the adipocyte and isolated subcellular fractions, the cultured fibroblast and hepatocyte...
  44. The Role of Novel Peroxins in Peroxisomal Biogenesis
    John Aitchison; Fiscal Year: 2007
    ..peroxisomes and two state-of-the-art complementary proteomics approaches applied to isolated peroxisomal subcellular fractions identified several previously uncharacterized putative peroxisomal proteins...
    Patrick Wong; Fiscal Year: 1980
    ..b>Subcellular fractions of the gingival tissue obtained from patients with peridontal disease will be used for assays of the ..
  46. A proteomics approach to Candida albicans biofilm
    WELDA CHAFFIN; Fiscal Year: 2005
    ..albicans cytoplasm Cell wall and cytoplasmic extracts will be examined and, time permitting other subcellular fractions may be examined if indicated by expression analysis derived from the parent grant...
    James Brown; Fiscal Year: 1991 used to localize the receptors ultrastructurally in the intact muscle and to characterize Ns and Ni in subcellular fractions isolated from the muscle...
    NORMAN RADIN; Fiscal Year: 1990
    ..We will develop an immunoassay for measuring their concentration in different brain cell types and subcellular fractions, organs, and fluids...
    Lynn Wecker; Fiscal Year: 1991
    ..and the esterified sources of chorine that provide precursor for ACh synthesis will be characterized in subcellular fractions from brain regions that contain a dense population of cholinergic nerve terminals, viz...
    Nigel Cooper; Fiscal Year: 1992
    ..retinal synaptic junction complexes (SJC), 3) lectin affinity isolated glycoproteins and 4) cone-specific subcellular fractions, all of which can be reliably isolated and characterized (progress report)...
    ROBERT DE LISLE; Fiscal Year: 1993
    ..term goal is to understand exocytosis at a molecular level by developing a model system reconstituted from subcellular fractions of the pancreatic acinar cell...
    Jennifer Stewart; Fiscal Year: 1990
    ..epinephrine, and to determine the relationship between changes in PMNT activity ad epinephrine turnover in subcellular fractions of discrete areas of the hypothalamus and brainstem...
    WALTER DANDLIKER; Fiscal Year: 1980
    ..fluorescence polarization kinetic and equilibrium measurements utilizing purified receptor preparations, subcellular fractions and whole cells under a variety of conditions of differing concentrations of the hormone, competitors, ..
    Ruedi Aebersold; Fiscal Year: 2002
    ..and global analysis of protein expression profiles in biological samples including biochemical and subcellular fractions, biological fluids, cells and tissues...
    GEORGE VAHOUNY; Fiscal Year: 1980
    ..b) adult heart cells (a preparation initially developed during the course of these studies); (c) specific subcellular fractions; and where feasible, (d) in vivo studies...
  56. LTQ XL Linear Ion Trap Mass Spectrometer
    Larry David; Fiscal Year: 2009
    ..mixtures of precipitated proteins in Protein/Protein or Protein/Drug interaction studies, preparations of subcellular fractions, extracts of microdissected tissues, and immuno-depleted serum samples...
    Michael Crow; Fiscal Year: 2007
    ..ARC is present in both subcellular fractions containing these organelles and staining reveals an association of ARC with the ER/developing sarcoplasmic ..
  58. Oxidant Mechanisms in Response to Ambient Particles
    Beatriz Gonzalez Flecha; Fiscal Year: 2006
    ..We will test CAPs effect on H2O2 production in cells treated with specific inhibitors, in subcellular fractions supplemented with their optimal substrates, and in genetically altered mice lacking or overexpressing ..
  59. PP2A-Like Constitutive ERK/MAPK Phosphatase in Brain
    Timothy Foley; Fiscal Year: 2003
    Lawrence Lash; Fiscal Year: 2003
    ..This information may have therapeutic applications for prevention of renal cellular injury or for understanding mitochondrial diseases or age-related decreases that occur in mitochondrial function. ..
    Lawrence Lash; Fiscal Year: 2007
    ..Achievement of these aims should build on our previous work in human kidney cells and extend it to provide a much more complete understanding of the various and complex ways in which DCVC affects the human kidney [unreadable] [unreadable]..
  62. Pneumocystis Biochemistry: Ubiquinones
    EDNA KANESHIRO; Fiscal Year: 2002
    ..carinii CoQ biosynthesis and growth in axenic cultures, and (4) develop atovaquone-resistant P. carinii populations to evaluate CoQ synthesis in sensitive and resistant organisms. ..