Genomes and Genes
Summary: A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Publications348 found, 100 shown here
- Phosphoinositides in cell regulation and membrane dynamicsGilbert Di Paolo
Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
Nature 443:651-7. 2006..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
- COPII and the regulation of protein sorting in mammalsGiulia Zanetti
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, California 94720, USA
Nat Cell Biol 14:20-8. 2012..In this review we cover aspects of mammalian COPII-mediated regulation of secretion, in particular related to the function of COPII paralogues, the spatial organization of cargo export and the role of accessory proteins...
- ARF proteins: roles in membrane traffic and beyondCRISLYN D'SOUZA-SCHOREY
Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
Nat Rev Mol Cell Biol 7:347-58. 2006....
- Functions of lipid rafts in biological membranesD A Brown
Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
Annu Rev Cell Dev Biol 14:111-36. 1998..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
- Organelle-specific initiation of cell death pathwaysK F Ferri
Centre National de la Recherche Scientifique, UMR 1599, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
Nat Cell Biol 3:E255-63. 2001..evidence suggests that other organelles, including the endoplasmic reticulum (ER), lysosomes and the Golgi apparatus, are also major points of integration of pro-apoptotic signalling or damage sensing...
- Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cellsTomohiro Uemura
Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
Cell Struct Funct 29:49-65. 2004..SNARE proteins were located on specific intracellular compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar ..
- ARF family G proteins and their regulators: roles in membrane transport, development and diseaseJulie G Donaldson
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Rev Mol Cell Biol 12:362-75. 2011..Important advances are being gained in our understanding of the functional networks that are formed not only by the GEFs and GAPs themselves but also by the inactive forms of the ARF proteins...
- Furin at the cutting edge: from protein traffic to embryogenesis and diseaseGary Thomas
Vollum Institute, 3181 SW Sam Jackson Park Road, Portland, Oregon 97239, USA
Nat Rev Mol Cell Biol 3:753-66. 2002..This review summarizes various features of furin--its structural and enzymatic properties, intracellular localization, trafficking, substrates, and roles in vivo...
- Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicleMarcus C S Lee
Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
Cell 122:605-17. 2005..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
- Golgi maturation visualized in living yeastEugene Losev
Department of Molecular Genetics and Cell Biology, and Institute for Biophysical Dynamics, The University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
Nature 441:1002-6. 2006The Golgi apparatus is composed of biochemically distinct early (cis, medial) and late (trans, TGN) cisternae. There is debate about the nature of these cisternae...
- Intracellular functions of N-linked glycansA Helenius
Institute of Biochemistry, , , , Switzerland
Science 291:2364-9. 2001..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
- Alpha-synuclein blocks ER-Golgi traffic and Rab1 rescues neuron loss in Parkinson's modelsAntony A Cooper
School of Biological Sciences, University of Missouri Kansas City, Kansas City, MO 64110, USA
Science 313:324-8. 2006..Thus, synucleinopathies may result from disruptions in basic cellular functions that interface with the unique biology of particular neurons to make them especially vulnerable...
- Models for Golgi traffic: a critical assessmentBenjamin S Glick
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA
Cold Spring Harb Perspect Biol 3:a005215. 2011....
- NKG2D ligand MICA is retained in the cis-Golgi apparatus by human cytomegalovirus protein UL142Omodele Ashiru
Department of Pathology, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
J Virol 83:12345-54. 2009..Here, we show that UL142 localizes predominantly to the endoplasmic reticulum (ER) and cis-Golgi apparatus. The transmembrane domain of UL142 mediates its ER localization, while we propose that the UL142 luminal ..
- Identification of a family of animal sphingomyelin synthasesKlazien Huitema
Department of Membrane Enzymology, Faculty of Chemistry, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
EMBO J 23:33-44. 2004..Whereas human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane. Collectively, these findings open up important new avenues for studying sphingolipid function in animals...
- Ras proteins: different signals from different locationsJohn F Hancock
Institute for Molecular Bioscience and Department of Molecular and Cellular Pathology, University of Queensland, Brisbane, Australia 4072
Nat Rev Mol Cell Biol 4:373-84. 2003..These different membrane microenvironments modulate Ras signal output and highlight the complex interplay between Ras location and function...
- A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeastM N Seaman
Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093 0668, USA
J Cell Biol 142:665-81. 1998..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval...
- Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACER Vassar
Amgen, Inc, One Amgen Center Drive, M S 29 2 B, Thousand Oaks, CA 91320 1799, USA
Science 286:735-41. 1999..Finally, the expression pattern and subcellular localization of BACE were consistent with that expected for beta-secretase. Future development of BACE inhibitors may prove beneficial for the treatment of Alzheimer's disease...
- Sedlin controls the ER export of procollagen by regulating the Sar1 cycleRossella Venditti
Telethon Institute of Genetics and Medicine, Naples, Italy
Science 337:1668-72. 2012..This joint action of TANGO1 and Sedlin sustained the ER export of PC, and its derangement may explain the defective chondrogenesis underlying SEDT...
- PtdIns4P recognition by Vps74/GOLPH3 links PtdIns 4-kinase signaling to retrograde Golgi traffickingChristopher S Wood
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, 19104, USA
J Cell Biol 187:967-75. 2009Targeting and retention of resident integral membrane proteins of the Golgi apparatus underly the function of the Golgi in glycoprotein and glycolipid processing and sorting...
- Role of oxysterol binding protein in hepatitis C virus infectionYutaka Amako
Department of Medicine, University of California, San Diego, La Jolla, 92093, USA
J Virol 83:9237-46. 2009..The pleckstrin homology (PH) domain located in the N-terminal region of OSBP targeted this protein to the Golgi apparatus. OSBP deletion mutation in the PH (DeltaPH) domain failed to localize to the Golgi apparatus and inhibited ..
- Coatomer-dependent protein delivery to lipid dropletsKrishnakant G Soni
Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
J Cell Sci 122:1834-41. 2009..Here we demonstrate that ATGL and ADRP, but not TIP47, are delivered to LDs by a pathway mediated by the COPI and COPII coatomer proteins and their corresponding regulators...
- Signals for sorting of transmembrane proteins to endosomes and lysosomesJuan S Bonifacino
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Biochem 72:395-447. 2003..This complex array of signals and recognition proteins ensures the dynamic but accurate distribution of transmembrane proteins to different compartments of the endosomal-lysosomal system...
- ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteinsH Van Valkenburgh
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
J Biol Chem 276:22826-37. 2001..These data reveal a complex network of interactions between GTPases in the ARF family and their effectors and reveal a potential for cross-talk not demonstrated previously...
- Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi networkAndrey Efimov
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Dev Cell 12:917-30. 2007..Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a centrosome-independent manner...
- Assembly and maturation of the flavivirus Kunjin virus appear to occur in the rough endoplasmic reticulum and along the secretory pathway, respectivelyJ M MacKenzie
Clinical Medical Virology Centre, University of Queensland, St Lucia, Queensland 4072, Australia
J Virol 75:10787-99. 2001..virions from the RER involves the transport of individual virions within individual vesicles en route to the Golgi apparatus. The results suggest that the assembly of virions occurs within the lumen of the RER and that subsequent ..
- Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiaeAniek van der Vaart
Department of Cell Biology and Institute of Biomembranes, University Medical Center Utrecht, Utrecht 3584, The Netherlands
Mol Biol Cell 21:2270-84. 2010..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism...
- Membrane lipids: where they are and how they behaveGerrit van Meer
Bijvoet Center and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
Nat Rev Mol Cell Biol 9:112-24. 2008..enzymes, translocases and transporters, plus the intrinsic physical phase behaviour of lipids and their interactions with membrane proteins, to create the unique compositions and multiple functionalities of their individual membranes?..
- Atg9 vesicles are an important membrane source during early steps of autophagosome formationHayashi Yamamoto
Frontier Research Center, Tokyo Institute of Technology, Yokohama 226 8503, Japan
J Cell Biol 198:219-33. 2012..majority of Atg9 existed on cytoplasmic mobile vesicles (designated Atg9 vesicles) that were derived from the Golgi apparatus in a process involving Atg23 and Atg27...
- Cargo-selective endosomal sorting for retrieval to the Golgi requires retromerMatthew N J Seaman
Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
J Cell Biol 165:111-22. 2004..The data presented here support the hypothesis that retromer performs a selective function in endosome-to-Golgi transport, mediating retrieval of the CI-MPR, but not furin...
- Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scatteringB Storrie
Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 0308, USA
J Cell Biol 143:1505-21. 1998During microtubule depolymerization, the central, juxtanuclear Golgi apparatus scatters to multiple peripheral sites...
- Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the GolgiYing Jie Wang
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA
Cell 114:299-310. 2003..We propose that PI4KIIalpha establishes the Golgi's unique lipid-defined organelle identity by generating PI(4)P-rich domains that specify the docking of the AP-1 coat machinery...
- Centrosome localization determines neuronal polarityFroylan Calderon de Anda
Cavalieri Ottolenghi Scientific Institute, Universita degli Studi di Torino, 10043 Orbassano, Torino, Italy
Nature 436:704-8. 2005..Centrosomes, the Golgi apparatus and endosomes cluster together close to the area where the first neurite will form, which is in turn opposite ..
- COPI acts in both vesicular and tubular transportJia Shu Yang
Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Cell Biol 13:996-1003. 2011....
- Ras signalling on the endoplasmic reticulum and the GolgiVi K Chiu
Department of Medicine, Cell Biology and Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
Nat Cell Biol 4:343-50. 2002..Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane...
- SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatusT Achstetter
Department of Biochemistry, University of California, Berkeley 94720
J Biol Chem 263:11711-7. 1988..cerevisiae with mutations at the sec7 locus are pleiotropically deficient in protein transport within the Golgi apparatus and proliferate a large array of Golgi cisternae at a restrictive growth temperature (37 degrees C)...
- The golgin Lava lamp mediates dynein-based Golgi movements during Drosophila cellularizationOphelia Papoulas
The Section of MCD Biology and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, TX 78712, USA
Nat Cell Biol 7:612-8. 2005..Our results provide new evidence that golgins promote dynein-based motility of Golgi membranes...
- Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not ArfsZhiqiu Man
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto, Japan
J Biol Chem 286:11569-78. 2011..These observations indicate that arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains...
- Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cellsMirita Franz-Wachtel
Proteome Center Tuebingen, University of Tuebingen, Tuebingen, Germany
Mol Cell Proteomics 11:160-70. 2012..This study presents the first global detection of PKD1-dependent phosphorylation events and provides a wealth of information for functional follow-up of PKD1 activity upon disruption of the Golgi network in human cells...
- A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathwayFélix E Rivera-Molina
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 106:14408-13. 2009..GEF cascades working in concert with counter-current GAP cascades could generate a programmed series of Rab conversions responsible for regulating the choreography of membrane traffic...
- Electron tomography reveals Rab6 is essential to the trafficking of trans-Golgi clathrin and COPI-coated vesicles and the maintenance of Golgi cisternal numberBrian Storrie
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
Traffic 13:727-44. 2012....
- TBC1D20 is a Rab1 GTPase-activating protein that mediates hepatitis C virus replicationElla H Sklan
Department of Medicine, Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Palo Alto, CA 94305 5187, USA
J Biol Chem 282:36354-61. 2007..These results highlight a novel mechanism by which viruses can hijack host cell machinery and suggest an attractive model whereby the NS5A-TBC1D20 interaction may promote viral membrane-associated RNA replication...
- Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene productsM N Seaman
Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093 0668, USA
J Cell Biol 137:79-92. 1997....
- A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to peripheryAndrea Burgo
Institut Jacques Monod, UMR 7592, CNRS, Universite Paris Diderot, Sorbonne Paris Cité, Paris, France
Dev Cell 23:166-80. 2012..The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface...
- The COPI system: molecular mechanisms and functionR Beck
Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520, USA
FEBS Lett 583:2701-9. 2009..Specifically, we will weigh the pros and cons of recent data on roles of the small GTP binding protein Arf1, of Arf1GAPs, and lipids during COPI carrier formation...
- Rab and actomyosin-dependent fission of transport vesicles at the Golgi complexS Miserey-Lenkei
Institut Curie, CNRS UMR144, Molecular mechanisms of intracellular transport Lab, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Nat Cell Biol 12:645-54. 2010..Our results provide evidence that the actomyosin system is required in vesicle biogenesis at the Golgi, and uncover a function for Rab GTPases in vesicle fission...
- Targeting of a chlamydial protease impedes intracellular bacterial growthJan G Christian
Institute for Medical Microbiology, Immunology and Hygiene, Technische Universitat Munchen, Munich, Germany
PLoS Pathog 7:e1002283. 2011..Recent work has shown that growth of Chlamydia induces the fragmentation of the Golgi apparatus (GA) into ministacks, which facilitates the acquisition of host lipids into the growing inclusion...
- A combinatorial genetic library approach to target heterologous glycosylation enzymes to the endoplasmic reticulum or the Golgi apparatus of Pichia pastorisJuergen H Nett
GlycoFi Inc, 21 Lafayette Street, Suite 200, Lebanon, NH 03766, USA
Yeast 28:237-52. 2011..This combinatorial approach, together with a high-throughput screening protocol, has allowed us to humanize the yeast glycosylation pathway to secrete human glycoprotein with complex N-glycosylation...
- Rab proteins mediate Golgi transport of caveola-internalized glycosphingolipids and correct lipid trafficking in Niemann-Pick C cellsAmit Choudhury
Department of Biochemistry and Molecular Biology, Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
J Clin Invest 109:1541-50. 2002..Our results demonstrate a role for Rab7 and Rab9 in the Golgi targeting of glycosphingolipids and suggest a new therapeutic approach for restoring normal lipid trafficking in NP-C cells...
- RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cellsJoanne Chia
Laboratory Regulation of Membrane Traffic, Institute of Molecular and Cell Biology, Singapore, Singapore
Mol Syst Biol 8:629. 2012The Golgi apparatus has many important physiological functions, including sorting of secretory cargo and biosynthesis of complex glycans. These functions depend on the intricate and compartmentalized organization of the Golgi apparatus...
- The multiple facets of the Golgi reassembly stacking proteinsFabian P Vinke
Cell Microscopy Centre, Department of Cell Biology, UMC Utrecht, The Netherlands
Biochem J 433:423-33. 2011..In the present paper, we review the multiple roles of GRASPs...
- TGN golgins, Rabs and cytoskeleton: regulating the Golgi trafficking highwaysBruno Goud
Institut Curie, CNRS UMR144, 26, rue d Ulm, Paris cedex 75248, France
Trends Cell Biol 20:329-36. 2010The architecture of the Golgi apparatus is intimately linked to its role in regulating membrane trafficking...
- The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasisAaron D Gitler
Whitehead Institute for Biomedical Research and Howard Hughes Medical Institute, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 105:145-50. 2008..Thus, alpha-syn causes general defects in vesicle trafficking, to which dopaminergic neurons are especially sensitive...
- GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote buddingHolly C Dippold
Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0707, USA
Cell 139:337-51. 2009..Consequently, this tensile force stretches the Golgi into the extended ribbon observed by fluorescence microscopy and the familiar flattened form observed by electron microscopy...
- The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complexHolger M Rohde
Boehringer Ingelheim Austria GmbH, Dr Boehringer Gasse 5 11, 1121 Vienna, Austria
J Biol Chem 278:52689-99. 2003..cerevisiae SAC1 gene encodes an integral membrane protein of the endoplasmic reticulum (ER) and the Golgi apparatus. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, ..
- Structural organization of the Golgi apparatusMartin Lowe
Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Oxford Road, Manchester M13 9PT, UK
Curr Opin Cell Biol 23:85-93. 2011The Golgi apparatus is a universal feature of eukaryotes, carrying out the key functions of processing, sorting and trafficking of newly synthesized membrane and secretory proteins...
- Mutations in dynein link motor neuron degeneration to defects in retrograde transportMajid Hafezparast
Department of Neurodegenerative Disease, Institute of Neurology, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK
Science 300:808-12. 2003..These mutations exclusively perturb neuron-specific functions of dynein...
- Multiple cytosolic and transmembrane determinants are required for the trafficking of SCAMP1 via an ER-Golgi-TGN-PM pathwayYi Cai
School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Plant J 65:882-96. 2011..Therefore, both the cytosolic N-terminus and TMD sequences of SCAMP1 play integral roles in mediating its transport to the PM via an ER-Golgi-TGN pathway...
- Interaction of tyrosine-based sorting signals with clathrin-associated proteinsH Ohno
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Science 269:1872-5. 1995..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery...
- Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturationMichael R Lang
Department of Medicine, Division of Genetic Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA
Nat Genet 38:1198-203. 2006..of the COPII complex, which is critical for anterograde protein trafficking between endoplasmic reticulum and Golgi apparatus. Zebrafish crusher mutants develop normally until the onset of craniofacial chondrogenesis...
- Live imaging of yeast Golgi cisternal maturationKumi Matsuura-Tokita
Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
Nature 441:1007-10. 2006There is a debate over how protein trafficking is performed through the Golgi apparatus. In the secretory pathway, secretory proteins that are synthesized in the endoplasmic reticulum enter the early compartment of the Golgi apparatus ..
- Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality controlSumin Han
Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
Proc Natl Acad Sci U S A 107:5851-6. 2010..Thus, the yeast Orm proteins control membrane biogenesis by coordinating lipid homeostasis with protein quality control...
- Vesicular transport: the core machinery of COPI recruitment and buddingWalter Nickel
Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
J Cell Sci 115:3235-40. 2002..The new findings have allowed us to generate a model that covers the cycle of coat recruitment, coat polymerization, vesicle budding and uncoating...
- Compartmental specificity of cellular membrane fusion encoded in SNARE proteinsJ A McNew
Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Nature 407:153-9. 2000..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis...
- Exocytosis of post-Golgi vesicles is regulated by components of the endocytic machineryJyoti K Jaiswal
The Rockefeller University, 1230 York Avenue, Box 304, New York, NY 10065, USA
Cell 137:1308-19. 2009..Our results demonstrate that these components of the endocytic machinery modulate the nature and extent of biosynthetic cargo delivery by post-Golgi vesicles at the cell membrane...
- Syntaxin 18, a SNAP receptor that functions in the endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle traffickingK Hatsuzawa
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192 0392, Japan
J Biol Chem 275:13713-20. 2000..Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi...
- Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosisM Mancini
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Cell Biol 149:603-12. 2000..We propose that the Golgi complex, like mitochondria, senses and integrates unique local conditions, and transduces pro-apoptotic signals through local caspases, which regulate local effectors...
- Quality control in the endoplasmic reticulumLars Ellgaard
Institute of Biochemistry, Swiss Federal Institute of Technology ETH Zurich, Honggerberg, CH 8093 Zurich, Switzerland
Nat Rev Mol Cell Biol 4:181-91. 2003..Here, we discuss recent progress in understanding the conformation-specific sorting of proteins at the level of ER retention and export...
- Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeastAyako Kita
Division of Molecular Pharmacology and Pharmacogenomics, Department of Genome Sciences, Kobe University Graduate School of Medicine, Kobe 650 0017, Japan
Mol Biol Cell 15:2920-31. 2004....
- Exiting the Golgi complexMaria Antonietta De Matteis
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro CH, Italy
Nat Rev Mol Cell Biol 9:273-84. 2008....
- Coordinated lipid transfer between the endoplasmic reticulum and the Golgi complex requires the VAP proteins and is essential for Golgi-mediated transportDiego Peretti
The Molecular Cell Biology Department, Weizmann Institute of Science, Rehovot 76100, Israel
Mol Biol Cell 19:3871-84. 2008..We propose that Nir2, OSBP, and CERT function coordinately at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities...
- A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organellesDror Avisar
Institute of Plant Sciences, Volcani Center, Bet Dagan 50250, Israel
Plant Physiol 150:700-9. 2009..Taken together, our data suggest that the above myosins are involved, directly or indirectly, in the movement of Golgi and mitochondria in plant cells...
- Arf6 and microtubules in adhesion-dependent trafficking of lipid raftsNagaraj Balasubramanian
Robert M Beirne Cardiovascular Research Center, Mellon Prostate Cancer Research Institute, University of Virginia, Charlottesville, Virginia 22908, USA
Nat Cell Biol 9:1381-91. 2007..Both of these steps are required for the plasma membrane targeting of Rac1 and for its activation. These data therefore define a new membrane raft trafficking pathway that is crucial for anchorage-dependent signalling...
- Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioningConnie Lam
Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK
J Cell Sci 123:202-12. 2010..Consistent with this hypothesis, expression of exogenous NDE1 or NDEL1 rescued the effects of LIS1 depletion on Golgi organisation, whereas LIS1 was only partially effective at rescuing the loss of NDE1 and NDEL1...
- Osh proteins regulate phosphoinositide metabolism at ER-plasma membrane contact sitesChristopher J Stefan
Weill Institute for Cell and Molecular Biology, Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Cell 144:389-401. 2011..Our findings further suggest that the conserved Osh proteins control PI metabolism at additional membrane contact sites...
- A comprehensive comparison of transmembrane domains reveals organelle-specific propertiesHayley J Sharpe
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
Cell 142:158-69. 2010..The pervasive presence of organelle-specific features among the TMDs of a particular organelle has implications for TMD prediction, regulation of protein activity by location, and sorting of proteins and lipids in the secretory pathway...
- Golgi-derived CLASP-dependent microtubules control Golgi organization and polarized trafficking in motile cellsPaul M Miller
Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
Nat Cell Biol 11:1069-80. 2009....
- The palmitoylation machinery is a spatially organizing system for peripheral membrane proteinsOliver Rocks
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69118 Heidelberg, Germany
Cell 141:458-71. 2010..This generic spatially organizing system differs from conventional receptor-mediated targeting mechanisms and efficiently counteracts entropy-driven redistribution of palmitoylated peripheral membrane proteins over all membranes...
- Nanoscale architecture of endoplasmic reticulum export sites and of Golgi membranes as determined by electron tomographyL Andrew Staehelin
Molecular Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Plant Physiol 147:1454-68. 2008
- A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expressionJie Cheng
Department of Physiology and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Biol Chem 277:3520-9. 2002..CAL is located primarily at the Golgi apparatus, co-localizing with trans-Golgi markers and is sensitive to Brefeldin A treatment...
- Golgin160 recruits the dynein motor to position the Golgi apparatusSmita Yadav
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
Dev Cell 23:153-65. 2012..One of the best-known examples is that of the mammalian Golgi apparatus, where constant inward movement of Golgi membranes results in its characteristic position near the centrosome...
- Characterization of a cis-Golgi matrix protein, GM130N Nakamura
Cell Biology Laboratory, Imperial Cancer Research Fund, London, UK
J Cell Biol 131:1715-26. 1995..Together these results suggest that GM130 is part of a cis-Golgi matrix and has a role in maintaining cis-Golgi structure...
- PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and functionMichelle M Hill
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
Cell 132:113-24. 2008..We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae...
- Secreted kinase phosphorylates extracellular proteins that regulate biomineralizationVincent S Tagliabracci
Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093 0721, USA
Science 336:1150-3. 2012..We identified a family of atypical protein kinases that localize within the Golgi apparatus and are secreted...
- Proteomics characterization of abundant Golgi membrane proteinsA W Bell
Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 2B2, Canada
J Biol Chem 276:5152-65. 2001A mass spectrometric analysis of proteins partitioning into Triton X-114 from purified hepatic Golgi apparatus (84% purity by morphometry, 122-fold enrichment over the homogenate for the Golgi marker galactosyl transferase) led to the ..
- Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 function in epidermal cell polarityJian Xu
Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands
Plant Cell 17:525-36. 2005..Fluorescence colocalization studies reveal that ARF1 is localized to the Golgi apparatus and endocytic organelles in both onion (Allium cepa) and Arabidopsis cells...
- Signalling to and from the secretory pathwayHesso Farhan
Biozentrum, University of Basel, Klingelbergstrasse 50 70, Basel, Switzerland
J Cell Sci 124:171-80. 2011..This Commentary highlights recent contributions to our understanding of the mutual regulation of the secretory pathway and cellular signalling...
- Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeastYan Ma
Division of Molecular Pharmacology and Pharmacogenomics, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kusunoki cho, Chuo Ku, Kobe 650 0017, Japan
Genes Cells 14:1015-28. 2009..Altogether, results suggest that the four subunits are all essential for the heterotetrameric complex formation and for the AP-1 function in exit transport from endosomes...
- Regulation of vascular endothelial growth factor receptor 2 trafficking and angiogenesis by Golgi localized t-SNARE syntaxin 6Venkatraman Manickam
Department of Anatomy and Cell Biology, University of Iowa, 51 Newton Rd, Iowa City, IA 52242, USA
Blood 117:1425-35. 2011..Plasma membrane (PM) levels of VEGFR2 are regulated by endocytosis and secretory transport through the Golgi apparatus. To date, the mechanism whereby the VEGFR2 traffics through the Golgi apparatus remains incompletely ..
- Is the 6 kDa tobacco etch viral protein a bona fide ERES marker?Alexander Lerich
Department of Plant Cell Biology, Centre for Organismal Studies, University of Heidelberg, Germany
J Exp Bot 62:5013-23. 2011..However, based on a consideration of the structure of its cytoplasmic tail, the scenario that the VP collects at ERES and is transported to the cis-Golgi before being recycled back to the ER, is supported...
- Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infectionRomain Grangeon
INRS Institut Armand Frappier, Institut National de la Recherche Scientifique, Laval, Quebec, Canada
J Virol 86:9255-65. 2012..TuMV infection led to the amalgamation of the endoplasmic reticulum (ER), Golgi apparatus, COPII coatamers, and chloroplasts into a perinuclear globular structure that also contained viral proteins...
- The N-terminal pro region mediates retention of unprocessed type-I PME in the Golgi apparatusSebastian Wolf
Heidelberg Institute for Plant Sciences, INF 360, 69120 Heidelberg, Germany
Plant J 58:361-75. 2009..indicate that the pro region operates as an effective retention mechanism, keeping unprocessed PME in the Golgi apparatus. Consequently, pro-protein processing could constitute a post-translational mechanism regulating PME activity.
- Phosphoinositide 3-kinase δ regulates membrane fission of Golgi carriers for selective cytokine secretionPei Ching Low
Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
J Cell Biol 190:1053-65. 2010..These findings demonstrate a new function for p110δ as part of the membrane fission machinery required at the TGN for the selective trafficking and secretion of cytokines in macrophages...
- A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatusCarmen Faso
Department of Energy, Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA
Plant Cell 21:3655-71. 2009How the endoplasmic reticulum (ER) and the Golgi apparatus maintain their morphological and functional identity while working in concert to ensure the production of biomolecules necessary for the cell's survival is a fundamental question ..
- Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3Subba Rao Gangi Setty
University of Pennsylvania School of Medicine, Department of Cell and Developmental Biology, 421 Curie Boulevard, BRB 2 3, Room 1010, Philadelphia, PA 19104 6058, USA
Curr Biol 13:401-4. 2003..The similar requirements for localization of GRIP domains from yeast, mouse, and human when expressed in yeast, and the presence of Arl1p and Arl3p homologs in these species, suggest that this is an evolutionarily conserved mechanism...
- The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domainFederica Brandizzi
Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, OX3 0BP Headington Oxford, United Kingdom
Plant Cell 14:1077-92. 2002..of the TM20-labeled compartments was investigated by coexpression with a marker localized mainly in the Golgi apparatus, AtERD2, fused to a yellow fluorescent protein...
- Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in ArabidopsisJacob Krüger Jensen
Laboratory of Molecular Plant Biology, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
Plant Cell 20:1289-302. 2008..The protein was shown by expression of a fluorescent fusion protein in N. benthamiana to be localized in the Golgi vesicles as expected for a glycosyltransferase involved in pectin biosynthesis...
- Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatusJae Sook Kang
Brain Korea 21, Division of Applied Life Science and Environmental Biotechnology National Core Research Center, Graduate School of Gyeongsang National University, Jinju 660 701, Korea
Proc Natl Acad Sci U S A 105:5933-8. 2008Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells...
- The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastidsAya Kitajima
Graduate School of Science and Technology, Niigata University, Niigata, Japan
Plant Cell 21:2844-58. 2009..Thus, Golgi-to-plastid traffic appears to be involved in the transport of glycoproteins to plastids and plastid targeting seems to be accomplished in a sorting signal-dependent manner...
- A myosin XI tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in Nicotiana benthamianaAmir Sattarzadeh
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
Mol Plant 2:1351-8. 2009..benthamiana. Taken together, these observations implicate myosin motor proteins in dynamics of plastids and stromules...
- Protein sorting to the storage vacuoles of plants: a critical appraisalDavid G Robinson
Heidelberg Institute for Plant Sciences, Cell Biology, University of Heidelberg, Im Neuenheimer Feld 230, D 69120 Heidelberg, Germany
Traffic 6:615-25. 2005..As a consequence, the plant Golgi apparatus is faced with the problem of recognizing proteins destined for lytic and storage vacuoles and segregating ..
- Phosphorylation of FGF23 coordinates crosstalk between the skeleton and kidneyVINCENT SCOTT TAGLIABRACCI; Fiscal Year: 2013..as Fam20C, and showed that it belongs to a novel family of atypical protein kinases that localize within the Golgi apparatus and are secreted...
- Regulation of K-Ras by a Farnesyl-electrostatic SwitchMARK REID PHILIPS; Fiscal Year: 2013..the plasma membrane and association with intracellular membranes, including the endoplasmic reticulum (ER), Golgi apparatus and the outer mitochondrial membrane...
- Misregulation of receptor tyrosine kinase signaling in PKDJordan A Kreidberg; Fiscal Year: 2013..Failure to ubquitinate c-Met is due to sequestration of the E3 ubiquitin ligase c-Cbl by 31 integrin in the Golgi apparatus. This grant will investigate the roles of c-Cbl, c-Met and protein glycosylation in the pathogenesis of PKD.
- Distance-Dependent Structure and Function of Neuronal DendritesKristen M Harris; Fiscal Year: 2010..that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) ..
- Elevated Zinc in Ischemia and reperfusionYang V Li; Fiscal Year: 2013..and to study the Zn2+ release from intracellular storages such as mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes...
- Molecular Mechanisms of Host Function Exploitation by Type IV effectors of LegionZhao Qing Luo; Fiscal Year: 2013..that several yeast proteins involved in membrane transport between the endoplasmic reticulum and the cis-Golgi apparatus can suppress the toxicity of SdeA. More importantly, in a screening to search for L...
- Membrane Protein Trafficking in PhotoreceptorsWolfgang Baehr; Fiscal Year: 2013..Integral membrane proteins are synthesized by ER-associated ribosomes and exported to the Golgi apparatus. Prenylated or acylated peripheral membrane proteins are synthesized in the cytosol and become ER-associated...
- Second-hit and sexual dimorphism effects in PAHPravin B Sehgal; Fiscal Year: 2013..of PAH have an increase in cell size ("megalocytosis"), characteristic cytoplasmic organellar changes (Golgi apparatus fragmentation, cystic dilatation of the endoplasmic reticulum) and an inability to handle intracellular ..
- Intraflagellar Transport Proteins in MiceGregory J Pazour; Fiscal Year: 2013..We have shown that IFT20 is required for ciliary assembly and that it cycles between the Golgi apparatus and the cilium. At the Golgi membrane, IFT20 is complexed with the golgin protein GMAP210...
- Fluorescent Copper Probes for Intracellular ImagingChristoph J Fahrni; Fiscal Year: 2013..Both imaging techniques will be applied to elucidate the role of the Golgi apparatus in copper redistribution and inheritance during mitosis and cell proliferation...
- STRUCTURAL BASIS OF ORGANELLE TETHERINGAdam D Linstedt; Fiscal Year: 2013DESCRIPTION (provided by applicant): The Golgi apparatus is compartmentalized into cisternae that associate with one another generating stacks...
- MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTIONSarah F Hamm-Alvarez; Fiscal Year: 2012....
- Role of Mechanisms of Efflux in Manganese Homeostasis and DetoxificationSomshuvra Mukhopadhyay; Fiscal Year: 2013..We have now identified an important role for efflux of Mn via uptake into the Golgi apparatus followed by secretion in maintaining cellular Mn levels and protecting against excess Mn accumulation during ..
- Regulation of Alcohol-induced Fatty Liver Disease by Lipid Droplet TraffickingMARK A MC NIVEN; Fiscal Year: 2010..The endoplasmic reticulum (ER), Golgi apparatus, and endosomal compartments have all been linked to lipid droplets...
- PtdIns 4-Kinase Regulation of Protein Sorting in the Golgi ApparatusKathryn M Ferguson; Fiscal Year: 2013DESCRIPTION (provided by applicant): The Golgi apparatus has two primary functions: biosynthesis of glycoproteins and glycolipids, and sorting...
- STRUCTURAL BASIS FOR SCAP/SREBP INTERACTIONJOSHUA JAMES ZIAREK; Fiscal Year: 2013..As sterol concentrations are reduced, SCAP escorts SREBP to the Golgi apparatus where it is activated...
- Mechanisms regulating PKGI proteolysis and modulation of pulmonary SMC phenotypeJesse D Roberts; Fiscal Year: 2013..The role of the Golgi apparatus in PKGI proteolysis and of active nuclear transport in PKGI[unreadable] in nuclear PKGI signaling will be ..
- The Role of Arf GTPases in Endocytosis and Postendocytic TransportJames E Casanova; Fiscal Year: 2010..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly ..
- INTEGRATED MODELING OF PROTEIN AND MEMBRANE TRAFFIC IN THE SECRETORY PATHWAYROBERT PHAIR; Fiscal Year: 2013..The endomembrane system itself consists of 3 major parts: the endoplasmic reticulum (ER), the Golgi apparatus and the plasma membrane, plus a complex series of internalization and recycling pathways grouped under the ..
- Structural Analysis of Golgi Trafficking ProteinsFrederick M Hughson; Fiscal Year: 2013..The Dsl1 complex functions in COPI vesicle transport from the Golgi apparatus to the endoplasmic reticulum (ER), a pathway essential for the recycling of the anterograde transport ..
- Functional mapping of SNARE-dependent trafficking and fusionJeffrey E Pessin; Fiscal Year: 2013..Thus, understanding the mechanism for this translocation process and the ability of insulin to increase glucose uptake are essentially process for the maintenance of normal glucose homeostasis. 1 ..
- Cyclic AMP Mediation of Epithelial Cell FunctionJAMES RICHARD GOLDENRING; Fiscal Year: 2012..AKAP350 is localized to both centrosomes and the Golgi apparatus, and we have demonstrated that depletion of AKAP350A with siRNA leads to disruption of the Golgi structure as ..
- THE ROLE OF THE SECRETORY PATHWAY IN CRANIOFACIAL MORPHOGENESISEla W Knapik; Fiscal Year: 2010..component of CORN vesicles, which take part in protein trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. A genetic lesion in the human SEC23A gene causes cranio-lenticulo-sutural dysplasia, which has similar ..
- Role of Rab Proteins in Golgi Apparatus Structure and FunctionBrian Storrie; Fiscal Year: 2013..Characterization of structural/functional relationships within the mammalian Golgi apparatus and its regulatory pathways is important to human health...
- A novel vacuolar compartment in Toxoplasma gondiiSilvia N Moreno; Fiscal Year: 2010..This work is designed to test the hypothesis that a multivesicular endosome present in these parasites is involved in trafficking of proteins to acidocalcisomes, and has a role in osmoregulation and intracellular pH homeostasis. ..
- Compartment Specific Signaling of RasMARK REID PHILIPS; Fiscal Year: 2010..last cycle of GM55779 we discovered that N-Ras and H-Ras become activated in fibroblasts on both the PM and Golgi apparatus. We mapped the pathway to activation on Golgi as one involving EGFR/PLC(/DAG + Ca++/RasGRP1...
- Targeting and Function of Golgi Membrane ProteinsCarolyn E Machamer; Fiscal Year: 2011..The proposed experiments on golgin-160 will add to our understanding of cargo delivery to the cell surface and to the role of the Golgi complex in transducing stress signals to the rest of the cell. ..
- The Roles of Host Factors in the AMPylation Activity of a Bacterial EffectorZhao Qing Luo; Fiscal Year: 2012..or more proteins involved in anterograde membrane transport between the endoplasmic reticulum (ER) and the Golgi apparatus. In the proposed study, we plan to: 1...
- A Small Molecule Approach Toward Understanding Bacterial Toxin TransportJOSE BERNARDO SAENZ; Fiscal Year: 2010..evidence suggests that it could serve as a useful tool in elucidating ARF1-mediated transport through the Golgi apparatus. In addition, we hope to explore the role of kinases in toxin trafficking...
- Regulation of Arf GTPase activation at the trans-Golgi networkJ Christopher Fromme; Fiscal Year: 2013..We expect that our research will lead to a clear model for how activation of Arf GTPases at the Golgi is regulated spatiotemporally. ..
- Epigenetic mechanisms and genes that regulate adipose tissue expansionRobert A Koza; Fiscal Year: 2013..genes, mesoderm specific transcript (Mest);a maternally imprinted gene localized in the endoplasmic reticulum/Golgi apparatus where it may function as an epoxide hydrolase, lipase or acyltransferase based on homology with members of ..
- BIOGENESIS OF THE GOLGI APPARATUSAdam D Linstedt; Fiscal Year: 2013....
- PHOSPHOLIPID DYNAMICS IN MEMBRANE ASSEMBLYDennis R Voelker; Fiscal Year: 2012..the genes and gene products involved in the export of phosphatidylethanolamine from mitochondria and the Golgi apparatus. In the fourth Specific Aim we will use recently developed conditional alleles for phosphatidylserine ..
- Mechanism of Targeting of a Luminal KDEL-containing Protein to the Golgi ComplexDEBORAH ARONSON; Fiscal Year: 2010..Our research will define new machinery that play a key role in promoting or preventing protein quality control. ..
- Cell cycle regulation of Golgi membrane dynamicsJoachim Seemann; Fiscal Year: 2013..In this application we propose to investigate the mechanisms that organize the division of the Golgi apparatus during mitosis...
- Toll-like receptor 9: trafficking and signalingCynthia A Leifer; Fiscal Year: 2013..This knowledge will provide the basis for the further development of CpG DNA as an adjuvant and therapeutic as well as develop mechanisms to interrupt the cycle of autoimmune pathology. ..
- The role of Rap1 and its novel associated molecules in T cell responsesVassiliki A Boussiotis; Fiscal Year: 2011..In T cells, TCR and CD28- mediated Ras activation occurs at the Golgi apparatus and requires phospholipase Cγ, whereas LFA-1- mediated Ras activation occurs at the plasma membrane ..
- Mechanisms of HTLV-I Tax-mediated NF-kB activationEdward W Harhaj; Fiscal Year: 2013..Tax activation of NF-kB correlates with ubiquitin-dependent relocalization of IKK to the Golgi apparatus. Tax is polyubiquitinated via Lys 63-linked ubiquitin chains and requires the ubiquitin conjugating enzyme ..
- Role of Phospholipid Remodeling in Secretion and Golgi Function in Mammalian CellWilliam J Brown; Fiscal Year: 2013..These studies will reveal novel biological roles for PLA and LPAT enzymes in mediating intracellular trafficking events in the secretory pathway and advance our understanding of membrane tubule-mediated transport. ..
- Regulation of Mitochondrial Release from Microtubules during Cell DivisionJarom Y Chung; Fiscal Year: 2013..However, proper cellular function also requires the partitioning of organelles. The mechanism behind ER, Golgi apparatus, and peroxisomes inheritance among others are better understood, but to date, that of mitochondrial ..
- Role of Retromer-mediated Retrograde Transport in HPV EntryDANIEL C DIMAIO; Fiscal Year: 2013..numerous novel essential factors, including several factors involved in retrograde transport to the Golgi apparatus. Prominent validated hits encode components of the retromer, a molecular machine involved in transport of ..
- Biogenesis and operation of the human cytomegalovirus assembly complexPhilip E Pellett; Fiscal Year: 2012..set of nested cylinders, with the outer cylinder consisting of networks of tubular vesicles derived from the Golgi apparatus and the trans-Golgi network;the inner cylinder consists of vesicles derived from recycling endosomes where ..
- Using Drosophila Neurons to Identify Mechanisms that Control Microtubule PolarityMelissa Rolls; Fiscal Year: 2013..Our results will form a foundation for understanding neurological diseases ranging from motor neuron disease to Williams syndrome, as they result from perturbations in microtubule organization or trafficking. ..
- Ubiquitin and Cell Cycle Regulation of Golgi Membrane DynamicsYanzhuang Wang; Fiscal Year: 2013..during mitosis, which are partitioned into the daughter cells where they are reassembled into a new Golgi apparatus. Reassembly is mediated by two ATPases (NSF and p97) that fuse the membranes...
- THE TRANSITIONAL ER-GOLGI SYSTEM IN BUDDING YEASTSBenjamin S Glick; Fiscal Year: 2012..This project focuses on the transitional ER (tER) and the Golgi apparatus. tER sites are membrane domains that produce COPII transport vesicles...
- Schizophrenia as a Disorder of Glutamate Receptor TraffickingJames H Meador-Woodruff; Fiscal Year: 2013..past 5 years support this model, with evidence for accelerated exit from the endoplasmic reticulum (ER) and Golgi apparatus of AMPA and NMDAR complexes including changes in N-linked glycosylation and expression of ER exit and ..
- Spatial Regulation of Cytoskeletal AsymmetryIrina Kaverina; Fiscal Year: 2012..We have recently demonstrated that a large number of microtubules originate from the Golgi apparatus (Efimov et al, 2007)...
- Sorting and trafficking in the endosomal systemChristopher G Burd; Fiscal Year: 2013..lipids in the endosome are packaged into transport carriers that bud from the endosome and ferry cargo to the Golgi apparatus and other organelles...
- Mechanisms of COPII-dependent TransportCHARLES K BARLOWE; Fiscal Year: 2013..Therefore these studies are basic for understanding numerous health related issues including cholesterol regulation, cystic fibrosis and diabetes. ..
- ROLE AND MECHANISMS OF EFFLUX IN MANGANESE HOMEOSTASIS AND DETOXIFICATIONSomshuvra Mukhopadhyay; Fiscal Year: 2013..The investigators have now identified an important role for efflux of Mn via uptake into the Golgi apparatus followed by secretion in maintaining cellular Mn levels and protecting against excess Mn accumulation during ..
- The Role of Ceramidases in Cancer ChemotherapyCungui Mao; Fiscal Year: 2013..These studies will provide insights into the role and mechanism of action of ACER2 and its product SPH in mediating anti-proliferative responses in cancer cells. ..
- Subcellular Mechanisms in Pathogenesis of Pulmonary Arterial HypertensionJASON EDWARD LEE; Fiscal Year: 2012..to the wrong subcellular compartments due to defects in intracellular trafficking at the level of the Golgi apparatus ("Golgi blockade hypothesis")...
- Structure and Function of Myosin VIH Lee Sweeney; Fiscal Year: 2013..it also can function as a strain-dependent anchor that is involved in organizing structures such as the Golgi apparatus. Myosin VI is found as a monomer in cells, but functions optimally as a dimer...
- Characterization of mammalian COG complex-interacting intra-Golgi trafficking macVladimir V Lupashin; Fiscal Year: 2012..analysis of the machinery that determines high-fidelity bidirectional protein and lipid trafficking in the Golgi apparatus. The functional interplay between SNAREs, Rabs and tethering factors will be investigated;we also hope to ..
- Live-Cell Assays of ER and Golgi Enzyme ActivitiesJOHN J NALEWAY; Fiscal Year: 2013..I project aims to develop new targeted fluorogenic substrates capable of measuring enzyme activities in the Golgi apparatus and Endoplasmic Reticulum (ER) of living cells and tissues...
- Intercellular Signaling and Endosome to Golgi Transport in Multicellular AnimalsRichard W Padgett; Fiscal Year: 2013..and understand the cellular components that transport internalized endocytic cargo from endosomes to the Golgi apparatus (retrograde recycling), and to understand how this process regulates, and is integrated within, intercellular ..
- Three dimensional aberration corrected scanning transmission electron microscopyDavid W Piston; Fiscal Year: 2011..g., ribosomes, proteasomes, Golgi apparatus, and mitochondria) within Eukaryotic cells...
- Super-Res Holographic Microscopy to Advance Research on Golgi Apparatus FunctionBrian Storrie; Fiscal Year: 2013..3. At Ben Gurion University (BGU), Rosen will continue to develop advanced algorithms and concepts based upon FINCH to improve performance and resolving power. ..
- Structural Studies of Lysosomal Storage DiseasesScott C Garman; Fiscal Year: 2013..where it must fold correctly;it must be post-translationally modified, allowing it to traffi through the Golgi apparatus to the lysosome;there, it must have the correct catalytic machinery and the stability to perform its ..
- ON THE ROLE OF THE HPS GENE PRODUCT IN MELANOCYTESRaymond Boissy; Fiscal Year: 2002..In common, these organelles either originate and/or receive specific glycoproteins from the Golgi apparatus. Thus it is hypothesized that the HPS gene product regulates selective trafficking from the trans Golgi ..
- ASSEMBLY OF NASCENT PLASMA LIPOPROTEINS BY HEPATOCYTESRobert Hamilton; Fiscal Year: 1991..The first is to thoroughly characterize nascent very low density lipoproteins (VLDL) isolated from the Golgi apparatus. The second is to determine the mechanism(s) by which nascent VLDL contribute to the formation of plasma high ..
- Mechanism of centrosome regulation from the GolgiChristine Suetterlin; Fiscal Year: 2013..of this project is to understand a control mechanism that regulates the centrosome from the adjacent Golgi apparatus in mammalian cells...
- Amyloid Beta-Protein: ApoE and Cholesterol HomeostasisWELLINGTON WOOD; Fiscal Year: 2009..Determine if the neuronal apoE4 and apoE-null phenotype can be "rescued" by incubation with astrocytes of apoE2, apoE3, or wildtype mice. In some experiments astrocytes will be treated with ABeta1-42. ..
- PENETRATION OF MACROMOLEULCES INTO CELLSH Ryser; Fiscal Year: 1999..by intracellular cleavage which, according to our preliminary data on cell fractionation, occurs in the Golgi apparatus. Our experimental approach will be: 1) to clarify the functional significance of the surface cleavage; 2) to ..
- GLYCOSYLTRANSFERASE FUNCTION DURING FERTILIZATIONBARRY SHUR; Fiscal Year: 2006..Finally, in the third Aim, we will continue our analysis of GalT-mediated signal transduction leading to the acrosome reaction. ..
- ENDOMEMBRANE TRAFFICKING OF FIV ENVELOPE GLYCOPROTEINSD Morre; Fiscal Year: 1993..proposal is to characterize the endomembrane trafficking between transitional endoplasmic reticulum and cis Golgi apparatus in lentivirus coat protein processing as a strategy for target identification in antiviral drug design using ..
- GLYCOSYLATION MECHANISMSCARLOS HIRSCHBERG; Fiscal Year: 2000..mechanisms which regulate the glycosylation of proteins and lipids in the rough endoplasmic reticulum and Golgi apparatus and, (b) to establish the role of protein and lipid glycosylation in organelle topography and ..