secretory vesicles

Summary

Summary: Vesicles derived from the GOLGI APPARATUS containing material to be released at the cell surface.

Top Publications

  1. ncbi Neutrophil granules and secretory vesicles in inflammation
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet 4042, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Microbes Infect 5:1317-27. 2003
  2. pmc Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
    Charles Boyd
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 167:889-901. 2004
  3. ncbi PKC-1 regulates secretion of neuropeptides
    Derek Sieburth
    Department of Molecular Biology, Simches 7, Massachusetts General Hospital, 185 Cambridge St, Boston, Massachusetts 02114, USA
    Nat Neurosci 10:49-57. 2007
  4. pmc Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage
    Ner Mu Nar Saw
    Division of Fundamental Neurobiology, University Health Network, Toronto, Ontario M5T 2S8, Canada
    Mol Biol Cell 22:3394-409. 2011
  5. ncbi Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
  6. pmc Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex
    Yui Jin
    Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 2216, USA
    Dev Cell 21:1156-70. 2011
  7. pmc The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans
    Samuel Liegeois
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Universite Louis Pasteur, 67400 Illkirch, France
    J Cell Biol 173:949-61. 2006
  8. ncbi Proteomics in gram-negative bacterial outer membrane vesicles
    Eun Young Lee
    Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
    Mass Spectrom Rev 27:535-55. 2008
  9. pmc Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length
    Daniel H Schott
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 156:35-9. 2002
  10. pmc Syntaxin clusters assemble reversibly at sites of secretory granules in live cells
    S Barg
    Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden
    Proc Natl Acad Sci U S A 107:20804-9. 2010

Research Grants

Detail Information

Publications364 found, 100 shown here

  1. ncbi Neutrophil granules and secretory vesicles in inflammation
    Mikkel Faurschou
    The Granulocyte Research Laboratory, Department of Haematology, Rigshospitalet 4042, University of Copenhagen, 9 Blegdamsvej, DK 2100 Copenhagen OE, Denmark
    Microbes Infect 5:1317-27. 2003
    The neutrophil is a major effector cell of innate immunity. Exocytosis of granules and secretory vesicles plays a pivotal role in most neutrophil functions from early activation to the destruction of phagocytosed microorganisms...
  2. pmc Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p
    Charles Boyd
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 167:889-901. 2004
    ..epifluorescence video microscopy indicate that all exocyst subunits, except for Sec3p, are associated with secretory vesicles as they arrive at exocytic sites...
  3. ncbi PKC-1 regulates secretion of neuropeptides
    Derek Sieburth
    Department of Molecular Biology, Simches 7, Massachusetts General Hospital, 185 Cambridge St, Boston, Massachusetts 02114, USA
    Nat Neurosci 10:49-57. 2007
    ..Similar neuropeptide secretion defects were found in mutants lacking unc-31 (encoding the protein CAPS) or unc-13 (encoding Munc13). These results suggest that PKC-1 selectively regulates DCV release from neurons...
  4. pmc Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage
    Ner Mu Nar Saw
    Division of Fundamental Neurobiology, University Health Network, Toronto, Ontario M5T 2S8, Canada
    Mol Biol Cell 22:3394-409. 2011
    ..Fluorescence-tagged and endogenous Voa1 are primarily localized on secretory vesicles, whereas fluorescence-tagged Voa2 and Voa3 are enriched on the Golgi and early endosomes, respectively...
  5. ncbi Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
    ..Moreover, the diversity of tethering complexes in the cell suggests that not all tethering events occur through the same mechanisms...
  6. pmc Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex
    Yui Jin
    Life Sciences Institute, Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109 2216, USA
    Dev Cell 21:1156-70. 2011
    ..Disruption of these interactions results in compromised growth and the accumulation of secretory vesicles. We identified the Sec15-binding region on Myo2 and also identified residues on Sec15 required for ..
  7. pmc The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans
    Samuel Liegeois
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sante et de la Recherche Medicale, Universite Louis Pasteur, 67400 Illkirch, France
    J Cell Biol 173:949-61. 2006
    ..These mutations allowed us to establish that the V0 sector mediates secretion of Hedgehog-related proteins. Our data raise the possibility that the V0 sector mediates exosome and morphogen release in mammals...
  8. ncbi Proteomics in gram-negative bacterial outer membrane vesicles
    Eun Young Lee
    Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
    Mass Spectrom Rev 27:535-55. 2008
    ....
  9. pmc Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length
    Daniel H Schott
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    J Cell Biol 156:35-9. 2002
    ..Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis...
  10. pmc Syntaxin clusters assemble reversibly at sites of secretory granules in live cells
    S Barg
    Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden
    Proc Natl Acad Sci U S A 107:20804-9. 2010
    ..Clusters facilitated exocytosis and disassembled once exocytosis was complete. Syntaxin cluster formation defines an intermediate step in exocytosis...
  11. pmc UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in Caenorhabditis elegans
    Marija Sumakovic
    European Neuroscience Institute Goettingen, German Research Foundation Research Center for Molecular Physiology of the Brain, 37077 Goettingen, Germany
    J Cell Biol 186:897-914. 2009
    ..Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation...
  12. ncbi Axonal transport of British and Danish amyloid peptides via secretory vesicles
    Seung Il Choi
    Department of Pathology, New York University School of Medicine, New York, New York, USA
    FASEB J 18:373-5. 2004
    ..cleavage, the BRI2-derived carboxyl-terminal peptides are transported via a regulated secretory pathway into secretory vesicles in neuronal cells...
  13. ncbi Molecular mechanism of myosin Va recruitment to dense core secretory granules
    Flora Brozzi
    Centre for Research in Biomedicine, Faculty of Health and Life Sciences, University of the West of England, Bristol, UK
    Traffic 13:54-69. 2012
    ..This study demonstrates that multiple binding partners of BR-MyoVa regulate SG transport, and this molecular mechanism is universally used by neuronal, endocrine and neuroendocrine cells...
  14. ncbi Functional and spatial segregation of secretory vesicle pools according to vesicle age
    Rory R Duncan
    Membrane Biology Group, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK
    Nature 422:176-80. 2003
    Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable...
  15. pmc Myosin VI and its binding partner optineurin are involved in secretory vesicle fusion at the plasma membrane
    Lisa M Bond
    Cambridge Institute for Medical Research, University of Cambridge, Cambridge CB20XY, United Kingdom
    Mol Biol Cell 22:54-65. 2011
    ..However, myosin VI and optineurin colocalize with secretory vesicles at the plasma membrane...
  16. pmc Syntaxin-6 SNARE involvement in secretory and endocytic pathways of cultured pancreatic beta-cells
    Regina Kuliawat
    Division of Endocrinology and Department of Developmental Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Biol Cell 15:1690-701. 2004
    ....
  17. pmc Annexin 2 promotes the formation of lipid microdomains required for calcium-regulated exocytosis of dense-core vesicles
    Sylvette Chasserot-Golaz
    Centre National de la Recherche Scientifique, Unité Propre de Recherche 2356, IFR 37 des Neurosciences, 67084 Strasbourg Cedex, France
    Mol Biol Cell 16:1108-19. 2005
    ..We propose that annexin 2 acts as a calcium-dependent promoter of lipid microdomains required for structural and spatial organization of the exocytotic machinery...
  18. pmc SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis
    T Lang
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    EMBO J 20:2202-13. 2001
    During exocytosis, SNARE proteins of secretory vesicles interact with the corresponding SNARE proteins in the plasmalemma to initiate the fusion reaction...
  19. pmc Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles
    Nele Alder-Baerens
    Institute of Biology and Biophysics, Humboldt University Berlin, 10115 Berlin, Germany
    Mol Biol Cell 17:1632-42. 2006
    ..Here, we show that yeast post-Golgi secretory vesicles (SVs) contain a translocase activity that flips NBD-PS, NBD-PE, and NBD-PC to the cytosolic leaflet...
  20. ncbi Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet
    Noriko Takahashi
    Department of Cell Physiology, National Institute for Physiological Sciences, and the Graduate University of Advanced Studies, Myodaiji, Okazaki 444 8585, Japan
    Science 297:1349-52. 2002
    ..When the pore dilated to 12 nanometers, the granules rapidly flattened and discharged their contents. Thus, our methodology reveals fusion pore dynamics in intact tissues at nanometer resolution...
  21. pmc Myosin Vc is a molecular motor that functions in secretory granule trafficking
    Damon T Jacobs
    Department of Cell and Molecular Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Biol Cell 20:4471-88. 2009
    ..These results provide the first live-cell imaging of Myo5c and indicate that Myo5c functions in secretory granule trafficking...
  22. ncbi Regulation of secretory vesicle traffic by Rab small GTPases
    M Fukuda
    Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba ku, Sendai, Miyagi 980 8578, Japan
    Cell Mol Life Sci 65:2801-13. 2008
    ..It is considered a specialized mode of membrane trafficking that is achieved by docking and fusion of secretory vesicles to the plasma membrane (i.e., exocytosis)...
  23. pmc Differential activity-dependent secretion of brain-derived neurotrophic factor from axon and dendrite
    Naoto Matsuda
    Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, University of California, Berkeley, California 94720, USA
    J Neurosci 29:14185-98. 2009
    ..Our study also revealed a novel mechanism by which differential exocytosis of BDNF-containing vesicles may regulate BDNF-TrkB signaling between connected neurons...
  24. pmc Cells release prions in association with exosomes
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:9683-8. 2004
    ..Furthermore, we found that exosomes bearing PrPsc are infectious. Our data suggest that exosomes may contribute to intercellular membrane exchange and the spread of prions throughout the organism...
  25. pmc Role for the actomyosin complex in regulated exocytosis revealed by intravital microscopy
    Andrius Masedunskas
    Intracellular Membrane Trafficking Unit and Proteases and Tissue Remodeling Section, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4340, USA
    Proc Natl Acad Sci U S A 108:13552-7. 2011
    ..Our results provide information on the machinery controlling regulated secretion and show that intravital microscopy provides unique opportunities to address fundamental questions in cell biology under physiological conditions...
  26. pmc Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network
    Robin W Klemm
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 185:601-12. 2009
    ..in membrane trafficking, we devised an immunoisolation procedure for specific recovery of post-Golgi secretory vesicles transporting a transmembrane raft protein from the TGN to the cell surface in the yeast Saccharomyces ..
  27. pmc Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast
    Darinel Ortiz
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA
    J Cell Biol 157:1005-15. 2002
    ..ts) mutations in sec2 and sec4 result in a tight block in secretion and the accumulation of secretory vesicles randomly distributed in the cell...
  28. pmc Regulated exocytosis in neuroendocrine cells: a role for subplasmalemmal Cdc42/N-WASP-induced actin filaments
    Stephane Gasman
    Centre National de la Recherche Scientifique Unité Propre de Recherche 2356, Institut Fédératif de Recherche 37, 67084 Strasbourg, France
    Mol Biol Cell 15:520-31. 2004
    ....
  29. pmc Multivesicular exocytosis in rat pancreatic beta cells
    M B Hoppa
    Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK
    Diabetologia 55:1001-12. 2012
    ..To establish the occurrence, modulation and functional significance of compound exocytosis in insulin-secreting beta cells...
  30. ncbi The roles of Rab27 and its effectors in the regulated secretory pathways
    Tetsuro Izumi
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Cell Struct Funct 28:465-74. 2003
    ..These findings suggest that the Rab27 subfamily regulates various exocytotic pathways using multiple organelle-specific effector proteins...
  31. ncbi Secretion of heparin-binding protein from human neutrophils is determined by its localization in azurophilic granules and secretory vesicles
    Hans Tapper
    Department of Cell and Molecular Biology, Lund University, Sweden
    Blood 99:1785-93. 2002
    ..of postnuclear supernatants from cavitated neutrophils showed that HBP is stored in azurophilic granules and secretory vesicles but that it is also detected to a minor extent in the plasma membrane...
  32. pmc The V-ATPase membrane domain is a sensor of granular pH that controls the exocytotic machinery
    Sandrine Poea-Guyon
    Centre de Neurosciences Paris Sud, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8195 and Université Paris Sud, F 91405 Orsay, France
    J Cell Biol 203:283-98. 2013
    ..Hence, the V-ATPase membrane domain would allow the exocytotic machinery to discriminate fully loaded and acidified vesicles from vesicles undergoing neurotransmitter reloading. ..
  33. ncbi New roles of myosin II during vesicle transport and fusion in chromaffin cells
    Patricia Neco
    Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, E 03550 Alicante, Spain
    J Biol Chem 279:27450-7. 2004
    ....
  34. ncbi Sorting ourselves out: seeking consensus on trafficking in the beta-cell
    Peter Arvan
    Division of Metabolism, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Traffic 5:53-61. 2004
    ..In this Traffic Interchange, the two co-authors attempt to reconcile their differences, leading to a common vision of proinsulin trafficking in primary and transformed cells...
  35. pmc Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis
    Kouichi Mizuno
    Laboratory of Molecular Endocrinology and Metabolism, Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Mol Biol Cell 22:1716-26. 2011
    ..These findings indicate that exophilin8 transiently traps insulin granules into the cortical actin network close to the microtubule plus-ends and supplies them for release during the stimulation...
  36. pmc A new role for the dynamin GTPase in the regulation of fusion pore expansion
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 22:1907-18. 2011
    ..These findings expand the membrane-sculpting repertoire of dynamin to include the regulation of immediate postfusion events in exocytosis that control the rate of release of soluble granule contents...
  37. pmc Quantifying neurite growth mediated by interactions among secretory vesicles, microtubules, and actin networks
    Krasimira Tsaneva-Atanasova
    Department of Computational Biology, Paris, France
    Biophys J 96:840-57. 2009
    ..Finally our study demonstrates that cytoskeletal dynamics is necessary to generate long protrusion, while vesicular delivery alone can only generate small neurite...
  38. ncbi Centrosome polarization delivers secretory granules to the immunological synapse
    Jane C Stinchcombe
    Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK
    Nature 443:462-5. 2006
    ..These data show that CTLs use a previously unreported mechanism for delivering secretory granules to the immunological synapse, with granule secretion controlled by centrosome delivery to the plasma membrane...
  39. pmc Discovery and progress in our understanding of the regulated secretory pathway in neuroendocrine cells
    Joelle Morvan
    London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London, UK
    Histochem Cell Biol 129:243-52. 2008
    ....
  40. ncbi Kiss and run exocytosis of dense core secretory vesicles
    Guy A Rutter
    Henry Wellcome Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK
    Neuroreport 15:79-81. 2004
    ....
  41. pmc Munc18-1 is critical for plasma membrane localization of syntaxin1 but not of SNAP-25 in PC12 cells
    Lakshmanan Arunachalam
    Division of Fundamental Neurobiology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, M5T 2S8, Canada
    Mol Biol Cell 19:722-34. 2008
    ..fact, recent studies of Munc18-1 mutants have suggested that Munc18-1 plays a critical role for docking of secretory vesicles, independent of syntaxin1 regulation...
  42. ncbi The Janus-faced nature of prostasomes: their pluripotency favours the normal reproductive process and malignant prostate growth
    G Ronquist
    Department of Medical Sciences, University Hospital, Uppsala, Sweden
    Prostate Cancer Prostatic Dis 7:21-31. 2004
    ....
  43. pmc Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 188:415-28. 2010
    ..We provide direct evidence for a persistent curvature in the exocytotic region that is altered by inhibition of dynamin guanosine triphosphatase activity and is temporally distinct from endocytosis measured by VMAT2-pHluorin...
  44. ncbi Selective recapture of secretory granule components after full collapse exocytosis in neuroendocrine chromaffin cells
    Mara Ceridono
    Institut des Neurosciences Cellulaires et Intégratives UPR 3212, Centre National de la Recherche Scientifique and Université de Strasbourg, 5 rue Blaise Pascal, F 67084 Strasbourg, France
    Traffic 12:72-88. 2011
    ..Altogether, these results suggest that after full collapse exocytosis, a selective sorting of granule membrane components is facilitated by the physical preservation of the granule membrane entity on the plasma membrane...
  45. pmc Proteomic analysis of microvesicles released by the human prostate cancer cell line PC-3
    Kirsten Sandvig
    Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital The Norwegian Radium Hospital, 0379 Oslo, Norway
    Mol Cell Proteomics 11:M111.012914. 2012
    ..Interestingly, our results show these proteins are promising biomarkers for prostate cancer and therefore candidates for clinical validation studies in biological fluids...
  46. ncbi Kiss-and-run exocytosis and fusion pores of secretory vesicles in human beta-cells
    Salma T Hanna
    Department of Pharmacology and Alberta Diabetes Institute, University of Alberta, HRIF East, Rm 6 126, Edmonton, AB T6G 2E1, Canada
    Pflugers Arch 457:1343-50. 2009
    Exocytosis of secretory vesicles results in the release of insulin from pancreatic beta-cells, although little is known about this process in humans...
  47. pmc A role for Rab7 in the movement of secretory granules in cytotoxic T lymphocytes
    Tiziana Daniele
    Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 0XY, UK
    Traffic 12:902-11. 2011
    ..Together, these results suggest that Rab7 may play a role in secretory lysosome movement toward the centrosome by interacting with RILP to recruit the minus-end motor, dynein...
  48. ncbi IQGAP1 involvement in MTOC and granule polarization in NK-cell cytotoxicity
    Namita Kanwar
    Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba, Canada
    Eur J Immunol 41:2763-73. 2011
    ..These results suggest previously undescribed role(s) for IQGAP1 in regulating multiple aspects of cytoskeletal organization and granule polarization in NK cells...
  49. pmc Vesicle-associated membrane protein 8 (VAMP8) is a SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) selectively required for sequential granule-to-granule fusion
    Natasha Behrendorff
    School of Biomedical Science, University of Queensland, QLD, Australia
    J Biol Chem 286:29627-34. 2011
    ..Taken together our data indicate that granule-to-granule fusion is regulated by VAMP8 containing SNARE complexes distinct from those that regulate primary granule fusion...
  50. pmc Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response
    Susanne J Bauman
    Duke University Medical Center, Department of Biochemistry, Box 3711, Research Drive, Durham, NC 27710, USA
    Microbes Infect 8:2400-8. 2006
    ..These results suggest that P. aeruginosa colonizing the CF lung may produce vesicles with a particular composition and that the vesicles could contribute to inflammation...
  51. pmc Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers
    M K Knowles
    Department of Chemistry and Biochemistry, University of Denver, Denver, CO 80208, USA
    Proc Natl Acad Sci U S A 107:20810-5. 2010
    Before secretory vesicles undergo exocytosis, they must recruit the proteins syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in the plasma membrane...
  52. ncbi Evidence of a role for Munc18-2 and microtubules in mast cell granule exocytosis
    Sophie Martin-Verdeaux
    Unite d Immuno Allergie, Institut Pasteur, Paris, France
    J Cell Sci 116:325-34. 2003
    ..These findings suggest a role for Munc18-2 and the microtubule network in the regulation of secretory granule dynamics in mast cells...
  53. pmc Neuropeptide delivery to synapses by long-range vesicle circulation and sporadic capture
    Man Yan Wong
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Cell 148:1029-38. 2012
    ..Therefore, vesicle circulation, which includes long-range retrograde transport and inefficient bidirectional capture, overcomes the limitations of one-way anterograde transport to uniformly supply release sites with DCVs...
  54. ncbi Induction of heat shock proteins in B-cell exosomes
    Aled Clayton
    Department of Clinical Oncology and Palliative Medicine, Wales College of Medicine, Cardiff University, Velindre Cancer Centre, Whitchurch, Cardiff, CF14 2TL, UK
    J Cell Sci 118:3631-8. 2005
    ....
  55. pmc Alpha-synuclein-induced aggregation of cytoplasmic vesicles in Saccharomyces cerevisiae
    James H Soper
    Center for Neurodegenerative Disease Research and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 19:1093-103. 2008
    ....
  56. pmc Biological functions and biogenesis of secreted bacterial outer membrane vesicles
    Adam Kulp
    Department of Biochemistry, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Microbiol 64:163-84. 2010
    ..Comparisons of similar budding mechanisms from diverse biological domains have provided new insight into evaluating mechanisms for outer membrane vesiculation...
  57. pmc TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells
    Mica Ohara-Imaizumi
    Department of Biochemistry II, Kyorin University School of Medicine, Shinkawa 6 20 2, Mitaka, Tokyo 181 8611, Japan
    Biochem J 381:13-8. 2004
    ..Thus our results indicate that the decreased number of docked insulin granules accounts for the impaired insulin release during the first phase of insulin release in diabetic GK rat beta-cells...
  58. pmc Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells
    Justin W Taraska
    Vollum Institute, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    Proc Natl Acad Sci U S A 100:2070-5. 2003
    ..Reacidification and dimming could be reversed by application of NH(4)Cl. We conclude that most granules reseal in <10 s after releasing cargo, and that these empty or partially empty granules are recaptured otherwise intact...
  59. pmc Sequential N- to C-terminal SNARE complex assembly drives priming and fusion of secretory vesicles
    Jakob B Sørensen
    Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Gottingen, Germany
    EMBO J 25:955-66. 2006
    ..We conclude that the SNARE complex forms in the N- to C-terminal direction, and that a partly assembled intermediate corresponds to the primed vesicle state...
  60. ncbi Exocytosis and cell polarity in plants - exocyst and recycling domains
    Viktor Zarsky
    Department of Plant Physiology, Charles University, Vinicna 5, 128 44 Praha 2, Czech Republic
    New Phytol 183:255-72. 2009
    ..However, in plant cells, there are obviously additional endosomal source compartments for secretory vesicles. The Rho/Rop GTPase regulatory module is central for the initiation of exocytotically active domains in ..
  61. ncbi Activity-dependent differential transmitter release in mouse adrenal chromaffin cells
    Tiberiu Fulop
    Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106 4970, USA
    J Neurosci 25:7324-32. 2005
    ..Thus, chromaffin cells may regulate release of different transmitters through a simple size-exclusion mechanism...
  62. ncbi Sequential-replenishment mechanism of exocytosis in pancreatic acini
    T Nemoto
    Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki 444-8585, Japan
    Nat Cell Biol 3:253-8. 2001
    ..This sequential-replenishment mechanism of exocytosis allows the cell to take advantage of a large supply of fusion-ready granules without needing to transport them to the plasma membrane...
  63. ncbi Biogenesis and transport of secretory granules to release site in neuroendocrine cells
    Joshua J Park
    Section on Cellular Neurobiology, Developmental Neurobiology Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, 20892, MD, USA
    J Mol Neurosci 37:151-9. 2009
    ..The cytoplasmic tail of CPE anchors secretory granules to the microtubule motors, kinesin-2 and -3, or dynein, via interaction with the adaptor, dynactin, to mediate anterograde and retrograde transport, respectively...
  64. ncbi The debate about transport in the Golgi--two sides of the same coin?
    H R Pelham
    MRC Laboratory of Molecular Biology, Cambridge, United Kingdom
    Cell 102:713-9. 2000
  65. pmc Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules
    Dietmar Riedel
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    Mol Cell Biol 22:6487-97. 2002
    ..of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland...
  66. ncbi Hypoxia stimulates vesicular ATP release from rat osteoblasts
    Isabel R Orriss
    Department of Cell and Developmental Biology, University College London, London, UK
    J Cell Physiol 220:155-62. 2009
    ..Moreover, increased extracellular ATP levels following acute exposure to low O(2) could influence local purinergic signaling and affect the balance between bone formation and bone resorption...
  67. ncbi Dynamics of subsynaptic vesicles and surface microclusters at the immunological synapse
    Marco A Purbhoo
    Section of Hepatology and Gastroenterology, Department of Medicine, Medical School Building, Imperial College London, London W2 1PG, UK
    Sci Signal 3:ra36. 2010
    ..Together, these data show that the movement of LAT-containing vesicles is linked to the organization of protein microclusters and suggest an important role for vesicular LAT in the SLP-76 signalosome...
  68. pmc Extracellular vesicles from Cryptococcus neoformans modulate macrophage functions
    Debora L Oliveira
    Laboratorio de Estudos Integrados em Bioquimica Microbiana, Instituto de Microbiologia Professor Paulo de Goes, Cidade Universitaria, Rio de Janeiro, Brazil
    Infect Immun 78:1601-9. 2010
    ..neoformans can stimulate macrophage function, apparently activating these phagocytic cells to enhance their antimicrobial activity. These results establish that cryptococcal vesicles are biologically active...
  69. ncbi ARF proteins: roles in membrane traffic and beyond
    CRISLYN D'SOUZA-SCHOREY
    Department of Biological Sciences and the Walther Cancer Institute, University of Notre Dame, Notre Dame, Indiana, USA
    Nat Rev Mol Cell Biol 7:347-58. 2006
    ....
  70. ncbi ICA69 is a novel Rab2 effector regulating ER-Golgi trafficking in insulinoma cells
    Laura Buffa
    Experimental Diabetology, Carl Gustav Carus School of Medicine, Dresden University of Technology, D 01307 Dresden, Germany
    Eur J Cell Biol 87:197-209. 2008
    ..Taken together, these data identify ICA69 as a novel Rab2 effector and point to its role in regulating the early transport of insulin secretory granule proteins...
  71. ncbi Proteomics analysis of insulin secretory granules
    Yannick Brunner
    Biomedical Proteomics Research Group, University Medical Center, 1211 Geneva 4, Switzerland
    Mol Cell Proteomics 6:1007-17. 2007
    ..The elucidation of their role will further help in the understanding of the mechanisms governing impaired insulin secretion during diabetes...
  72. ncbi Secretory cytotoxic granule maturation and exocytosis require the effector protein hMunc13-4
    Mickaël M Ménager
    Institut National de la Santé et de la Recherche Médicale Unité 768, Laboratoire du Développement Normal et Pathologique du Système Immunitaire, Paris F 75015 France
    Nat Immunol 8:257-67. 2007
    ..Our data provide insight into the regulation of the generation and release of cytotoxic granules by effector cytotoxic T lymphocytes and natural killer cells...
  73. ncbi Ral GTPases regulate exocyst assembly through dual subunit interactions
    Serge Moskalenko
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9039, USA
    J Biol Chem 278:51743-8. 2003
    ..complex, or exocyst, a multisubunit complex facilitating post-Golgi targeting of distinct subclasses of secretory vesicles, has been identified as a bona fide Ral effector complex...
  74. pmc The exocyst complex associates with microtubules to mediate vesicle targeting and neurite outgrowth
    I E Vega
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Neurosci 21:3839-48. 2001
    ..complex and microtubule redistribution, abolishing neurite outgrowth and promoting cytosolic accumulation of secretory vesicles. Consistently, the overexpression of Exocyst sec10 subunit mutant blocks neurite outgrowth...
  75. ncbi Are rhoptries in Apicomplexan parasites secretory granules or secretory lysosomal granules?
    Huan M Ngo
    Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520 8022, USA
    Mol Microbiol 52:1531-41. 2004
    ..Current findings suggest that rhoptries receive products from both biosynthetic and endocytic pathways and, therefore, they are most analogous to secretory lysosomal granules found in mammalian cells...
  76. ncbi Interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules
    Masahiro Hosaka
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    J Cell Sci 118:4785-95. 2005
    ..We suggest that SgIII and CPE form the separate functional sorting complex by anchoring to cholesterol-rich SG membranes, and POMC-derived peptides are transferred from CPE to SgIII, and subsequently to CgA...
  77. pmc Corelease and differential exit via the fusion pore of GABA, serotonin, and ATP from LDCV in rat pancreatic beta cells
    Matthias Braun
    Oxford Center for Diabetes, Endocrinology, and Metabolism, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, UK
    J Gen Physiol 129:221-31. 2007
    ....
  78. ncbi Actin is involved in pollen tube tropism through redefining the spatial targeting of secretory vesicles
    Firas Bou Daher
    Department of Biological Sciences, Institut de recherche en biologie végétale, University of Montreal, Montreal, Quebec H1X 2B2, Canada
    Traffic 12:1537-51. 2011
    ..Together these results suggest that the precise targeting of the delivery of new wall material represents a key component in the growth machinery that determines directional elongation in pollen tubes...
  79. ncbi Cell-specific targeting of caveolin-1 to caveolae, secretory vesicles, cytoplasm or mitochondria
    W P Li
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA
    J Cell Sci 114:1397-408. 2001
    ..We conclude that caveolin-1 can be targeted to a variety of intracellular destinations, which suggests a novel mechanism for the intracellular traffic of this protein...
  80. pmc Intersectin-1L nucleotide exchange factor regulates secretory granule exocytosis by activating Cdc42
    Magali Malacombe
    Département Neurotransmission and Sécrétion Neuroendocrine, Institut des Neurosciences Cellulaires et Intégratives UMR 7168 LC2, Centre National de la Recherche Scientifique and Université Louis Pasteur, Strasbourg, France
    EMBO J 25:3494-503. 2006
    ..Our results extend the current role of intersectin-1L in endocytosis to a function in exocytosis and support the idea that intersectin-1L is an adaptor that coordinates exo-endocytotic membrane trafficking in secretory cells...
  81. pmc Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy and fluorescence recovery after photobleaching
    Jérôme Bove
    Departement de sciences biologiques, Universite de Montreal, Montreal, Quebec, Canada H1X 2B2
    Plant Physiol 147:1646-58. 2008
    ..Therefore, we propose that more than one passage into the apex may be needed for many vesicles before they fuse to the plasma membrane and deliver their contents...
  82. ncbi Long-term exposure to glucose and lipids inhibits glucose-induced insulin secretion downstream of granule fusion with plasma membrane
    Charlotta S Olofsson
    Department of Clinical Sciences, Clinical Research Centre, Lund University, Malmo, Sweden
    Diabetes 56:1888-97. 2007
    ..We conclude that glucose and FFAs reduce insulin secretion by interference with the exit of insulin via the fusion pore...
  83. ncbi Myosin Va facilitates the distribution of secretory granules in the F-actin rich cortex of PC12 cells
    Rudiger Rudolf
    Department of Neurobiology, Interdisciplinary Center of Neuroscience, University of Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
    J Cell Sci 116:1339-48. 2003
    ....
  84. ncbi Selective nucleotide-release from dense-core granules in insulin-secreting cells
    Stefanie Obermuller
    Department of Experimental Medicinal Sciences, Lund University, BMC B11, SE 221 84 Lund, Sweden
    J Cell Sci 118:4271-82. 2005
    ..These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone.
  85. ncbi A subset of p23 localized on secretory granules in pancreatic beta-cells
    Masahiro Hosaka
    Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Histochem Cytochem 55:235-45. 2007
    ..These findings suggest that a fraction of p23 enters post-Golgi compartments and may function in the biogenesis and/or quality control of SGs...
  86. ncbi Maturation of secretory granules
    Tanja Kogel
    Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009, Bergen, Norway
    Results Probl Cell Differ 50:1-20. 2010
    ..Furthermore, live cell imaging in combination with fluorescently tagged marker proteins of SGs provided insights into the dynamics of maturing ISGs, and the functional implications of cytoskeletal elements and motor proteins...
  87. ncbi Protein kinase C mediates platelet secretion and thrombus formation through protein kinase D2
    Olga Konopatskaya
    School of Physiology and Pharmacology, Bristol Heart Institute, Bristol, United Kingdom
    Blood 118:416-24. 2011
    ..Our results show that in the molecular pathway to secretion, PKD2 is a key component of the PKC-mediated pathway to platelet activation and thrombus formation through its selective regulation of dense granule secretion...
  88. pmc The class V myosin motor, myosin 5c, localizes to mature secretory vesicles and facilitates exocytosis in lacrimal acini
    RONALD R MARCHELLETTA
    Department Pharmacology and Pharmaceutical Sciences, USC School of Pharmacy, Los Angeles, CA 90033, USA
    Am J Physiol Cell Physiol 295:C13-28. 2008
    ..Confocal fluorescence and immunogold electron microscopy revealed that Myo5c was associated with secretory vesicles in primary rabbit LGAC...
  89. pmc Cell type-dependent trafficking of neuropeptide Y-containing dense core granules in CNS neurons
    Prabhu Ramamoorthy
    Department of Biology, Pennsylvania State University, State College, Pennsylvania 16802, USA
    J Neurosci 31:14783-8. 2011
    ..These results indicate that the trafficking of neuropeptide-containing dense core granules is markedly cell type specific and is not determined entirely by the characteristics of the particular peptide per se...
  90. pmc Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis
    Qiansheng Ren
    Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY 40536, USA
    Blood 116:869-77. 2010
    ..These data highlight the importance of Munc13-4 in platelets and indicate that it is a limiting factor required for platelet secretion and hemostasis...
  91. ncbi The yeast Rvs161 and Rvs167 proteins are involved in secretory vesicles targeting the plasma membrane and in cell integrity
    A M Breton
    Laboratoire de Biologie Cellulaire de la Levure, LBCL IBGC CNRS UMR 5095, 1 rue Camille Saint Saens, F 33077 Bordeaux cedex, France
    Yeast 18:1053-68. 2001
    ..The rvs mutants accumulate late secretory vesicles at sites of membrane and cell wall construction...
  92. pmc Small G proteins as key regulators of pancreatic digestive enzyme secretion
    John A Williams
    Dept of Molecular and Integrative Physiology, Univ of Michigan, Ann Arbor, MI 48109, USA
    Am J Physiol Endocrinol Metab 296:E405-14. 2009
    ..The Rho family small G proteins RhoA and Rac1 also regulate secretion through remodeling of the actin cytoskeleton. Possible mechanisms for regulation of these G proteins and their effector molecules are considered...
  93. pmc Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles
    Bo Zhao
    Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    Cell Res 21:741-53. 2011
    ..Thus, LDCVs serve as carriers of receptors, ion channels and preassembled receptor signaling complexes, enabling a rapid, activity-dependent modulation of neuronal sensitivity...
  94. ncbi Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes
    Jasmin Herz
    Institute for Medical Microbiology, Immunology and Hygiene, Medical Center, University of Cologne, Germany
    Nat Immunol 10:761-8. 2009
    ..In ASMase-KO CD8+ T cells, the contraction of secretory granules was markedly impaired. Thus, ASMase is required for contraction of secretory granules and expulsion of cytotoxic effector molecules...
  95. ncbi Insulin granule trafficking in beta-cells: mathematical model of glucose-induced insulin secretion
    Alessandro Bertuzzi
    Institute of Systems Analysis and Computer Science, Consiglio Nazionale delle Ricerche, Rome
    Am J Physiol Endocrinol Metab 293:E396-409. 2007
    ..Model parameter changes that may reflect alterations of beta-cell function present in type 2 diabetes are investigated, and the action of pharmacological agents that bind to sulfonylurea receptors is simulated...
  96. pmc VAMP8 is the v-SNARE that mediates basolateral exocytosis in a mouse model of alcoholic pancreatitis
    Laura I Cosen-Binker
    Department of Medicine, University of Toronto and University Health Network, Toronto, Ontario, Canada
    J Clin Invest 118:2535-51. 2008
    ..These findings indicate that VAMP8 is the ZG-SNARE that mediates basolateral exocytosis in alcoholic pancreatitis and that VAMP8 is critical for ZG-ZG homotypic fusion...
  97. pmc Chronic palmitate exposure inhibits insulin secretion by dissociation of Ca(2+) channels from secretory granules
    Michael B Hoppa
    The Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Oxford OX3 7LJ, UK
    Cell Metab 10:455-65. 2009
    ..In both mouse and human islets, the palmitate-induced secretion defect was reversed when the beta cell action potential was pharmacologically prolonged...
  98. ncbi A role for the transcriptional repressor REST in maintaining the phenotype of neurosecretory-deficient PC12 cells
    Alena Pance
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    J Neurochem 99:1435-44. 2006
    ..Our work identifies one of the mechanisms underlying the phenotype of neurosecretory-deficient neuroendocrine cells, and begins to define the critical components that determine a key aspect of the neuroendocrine phenotype...
  99. pmc Direct interaction between Rab3D and the polymeric immunoglobulin receptor and trafficking through regulated secretory vesicles in lacrimal gland acinar cells
    Eunbyul Evans
    Dept of Pharmacology and Pharmaceutical Sciences, Univ of Southern California, Los Angeles, CA 90089 9121, USA
    Am J Physiol Cell Physiol 294:C662-74. 2008
    ..These data suggest that the novel localization of pIgR to the regulated secretory pathway of LGACs and its secretion therefrom may be affected by its novel interaction with Rab3D...
  100. ncbi Inhibition of cathepsin B reduces beta-amyloid production in regulated secretory vesicles of neuronal chromaffin cells: evidence for cathepsin B as a candidate beta-secretase of Alzheimer's disease
    Vivian Hook
    Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA
    Biol Chem 386:931-40. 2005
    ..Previously, cysteine protease activity was demonstrated as the major beta-secretase activity in regulated secretory vesicles of neuronal chromaffin cells...
  101. ncbi Short-term plasticity of small synaptic vesicle (SSV) and large dense-core vesicle (LDCV) exocytosis
    Yongsoo Park
    Department of Life Science, Pohang University of Science and Technology, Pohang 790 784, Republic of Korea
    Cell Signal 21:1465-70. 2009
    ..Short-term plasticity of SSV exocytosis results from Ca2+ accumulation, but ADP of LDCV exocytosis does not. Here, we review the signaling mechanisms and differences of short-term plasticity in exocytotic processes of SSV and LDCV...

Research Grants65

  1. BONE MATRIX AND BONE RESORPTION
    Paul R Odgren; Fiscal Year: 2012
    ..OCs and will screen candidate proteins for potential roles in a complex needed to translocate OC secretory vesicles to the RB and secrete their contents into the resorption lacuna to effect bone resorption. Under SA1...
  2. Serotonin transporter-mediated regulation of neuroendocrine exocytosis
    KEVIN P CURRIE; Fiscal Year: 2013
    ..This will provide the foundation for future expanded applications to investigate a new paradigm for 5HT / SERT mediated signal transduction. ..
  3. Mechanism of Neutrophil Activation
    Kenneth R McLeish; Fiscal Year: 2013
    ..Specific Objective 1 will test the working hypothesis that exocytosis of secretory vesicles and gelatinase granules results in increased plasma membrane expression of p91phox and p22phox, which is ..
  4. Exocytosis and Coupled Endocytosis in Neuroendocrine Cells
    JOHN DAVID CASTLE; Fiscal Year: 2010
    ..4) Exocytosis and exo- endocytic coupling will be analyzed in chromaffin cells lacking SCAMP1 using amperometry, tracer uptake, and electron microscopy to analyze defects thought to be related to chromaffin cell physiology. ..
  5. The exocyst in ciliogenesis and cystogenesis
    Joshua H Lipschutz; Fiscal Year: 2013
    ..is crucial for proper cell function and the exocyst is known for mediating the targeting and docking of secretory vesicles carrying membrane proteins. Multiple Rho and Rab family GTPases regulate the exocyst...
  6. Secretory Vesicle Function During Spermatogenesis & Fertilization in C. elegans
    STEVEN W L apos HERNAULT; Fiscal Year: 2010
    ..Sperm secretory vesicles acquire an acidic internal pH during their maturation due to vacuolar (V-) ATPase activity, and this occurs ..
  7. MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE
    Anthony P Bretscher; Fiscal Year: 2013
    ..these questions, the researchers use budding yeast where the essential myosin-V, encoded by Myo2, transports secretory vesicles for growth as its major cargo, but also transports peroxisomes, compartments of the secretory pathway, and ..
  8. Regulation of Cellular Zn Redistribution: A Role of ZnT2
    Shannon L Kelleher; Fiscal Year: 2012
    ..that prolactin regulates discrete ZnT2 isoforms to redistribute cellular Zn pools from mitochondria to secretory vesicles to facilitate extraordinary Zn secretion in highly specialized secretory tissues...
  9. Trafficking mechanisms for secretory vesicles in pancreatic duct epithelial cells
    Duk Su Koh; Fiscal Year: 2013
    ..We will start by examining the effect of the PAR-2-generated intracellular Ca2+ signal on the trafficking of secretory vesicles (SV) that carry mucin or membrane transporters responsible for bicarbonate secretion...
  10. MICROTUBULE-BASED TRANSPORT IN LACRIMAL GLAND FUNCTION
    Sarah F Hamm-Alvarez; Fiscal Year: 2012
    ..cells (LGAC), the major source of tear proteins, packaging of materials destined for apically-targeted secretory vesicles (SV) occurs in the trans-Golgi network (TGN)...
  11. The roles of acidic autophagosomes in production of infectious poliovirus
    William T Jackson; Fiscal Year: 2013
    ..of this proposal is that PV infection generates autophagosomes through morphogenesis of COPII-like secretory vesicles used for RNA replication, and acidification of the newly formed autophagosomes promotes virion maturation...
  12. Regulation of Synaptic Transmission by Gq
    Michael Ailion; Fiscal Year: 2013
    ..Thus, the new genes identified in my work are good candidates for genes linked to mental health disease in humans. ..
  13. Estrogen-Induced Hippocampal Seizure Susceptibility
    Catherine S Woolley; Fiscal Year: 2013
    ..Understanding the mechanisms by which estrogen influences hippocampal synaptic function is important to identify cellular and molecular targets for novel anti-seizure therapies. ..
  14. The Function of the Mia3 Gene in Murine Placentation
    Ann E Sutherland; Fiscal Year: 2012
    ..TANGO1 in cultured human cells functions to facilitate loading of COPII coated secretory vesicles with specific cargo proteins, thus I hypothesize that the Xst199 mutation of Tango1 in mouse embryos ..
  15. Mechanisms of fusion and transmitter release in neurosecretion
    Manfred Lindau; Fiscal Year: 2012
    ..Upon stimulation the contents of the secretory vesicles are released through a fusion pore that connects the vesicular lumen to the extracellular space...
  16. Regulation of Myosin V Interaction with Cargo
    Lois S Weisman; Fiscal Year: 2013
    ..cargo binding regions;one required for binding to the yeast vacuole, the other required for binding to secretory vesicles. We found that the vacuole binding site interacts with a novel protein, Vac17...
  17. Regulation of exocyst-mediated post-Golgi vesicle tethering
    Kelly Orlando; Fiscal Year: 2009
    ..Exocytosis occurs when post-Golgi secretory vesicles are tethered to and fuse with the plasma membrane (PM)...
  18. Molecular Basis of Polarized Exocytosis
    Wei Guo; Fiscal Year: 2012
    ..The "exocyst" is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles to specific domains of the plasma membrane for exocytosis...
  19. Host cell manipulation by Toxoplasma
    Isabelle Coppens; Fiscal Year: 2013
    ..into Golgi ministacks that align along the PV and intercepts the Golgi vesicular trafficking by engulfing secretory vesicles into the PV. This strategy may allow the parasite to retrieve Golgi lipids from sequestered Golgi vesicles...
  20. Molecular Mechanisms Controlling Postsynaptic Secretion
    Anton Maximov; Fiscal Year: 2013
    ..and high-resolution live cell imaging, we will determine whether Syt-11 and BDNF co-traffic in the same secretory vesicles. Importantly, we will identify the sites of vesicle exocytosis and determine how exocytosis correlates with ..
  21. Type-1 Diabetes: Zn2+ Potentiated Beta-Cell Death By Sirtuin-Mediated NAD+ Loss
    CHRISTIAN THOMAS SHELINE; Fiscal Year: 2010
    ..Zinc transporter 5 (ZnT5) was suggested to load Zn2+ into secretory vesicles in beta-cells...
  22. Aminopeptidases for Neurotoxic Pyroglutamate Beta-Amyloid of Alzheimers Disease
    Vivian Y H Hook; Fiscal Year: 2013
    ..by our preliminary data demonstrating the presence of aspartate and alanine aminopeptidase activities in secretory vesicles that contain pGluA[unreadable] (3-40/42) and A[unreadable] (1-40/42)...
  23. Formation of the Regulated Secretory Pathway
    Robert H Edwards; Fiscal Year: 2013
    ..We will also extend the analysis to mice lacking AP-3 and VPS41. The results will provide a foundation for future work on the molecular mechanisms involved in LDCV formation and the consequences for physiology, development and disease. ..
  24. Molecular Mechanisms of Endocytosis
    Andrew J Bean; Fiscal Year: 2010
    ..retrieve and sort molecules from the plasma membrane as a result of the fusion of regulated and constitutive secretory vesicles. The endocytic pathway can be separated into numerous stages based on the movement of cargo and the ..
  25. Regulation of insulin secretion C. elegans
    Joshua M Kaplan; Fiscal Year: 2013
    ..These studies should provide new insights into the cellular mechanisms regulating secretion of neuropeptides sleep, and arousal. ..
  26. Structural studies of the exocyst
    NEIL VASAN; Fiscal Year: 2011
    b>Secretory vesicles bud from the trans-Golgi and move along cytoskeletal elements to sites of active secretion...
  27. Regulating BDNF Action in Postnatal Development.
    Barbara L Hempstead; Fiscal Year: 2013
    ..tagged mouse, identify if conversion of proBDNF to mature BDNF occurs during synthesis and sorting to secretory vesicles, or whether conversion occurs following vesicle release...
  28. Roles for ER Stress Inducible Cardiomyokines in the heart
    Christopher C Glembotski; Fiscal Year: 2013
    ..Most CMKs are synthesized and folded in the rough ER, routed to the Golgi, then to secretory vesicles before secretion...
  29. The Role of Mmr1p in Cargo Delivery by Myo2p
    IRINA S CHERNYAKOV; Fiscal Year: 2012
    ..The essential function of Myo2p is in the transport of secretory vesicles into the bud for cell growth, but it also transports many organelles into the bud for their segregation ..
  30. CHANNELS, CALCIUM AND PEPTIDE SECRETION
    Edwin S Levitan; Fiscal Year: 2013
    ..Furthermore, the proposal will reveal how these processes are affected by acute nerve injury and diseases such as Fragile X syndrome and Lou Gehrig's disease (ALS). ..
  31. Measurement of physiological secretory vesicle-plasma membrane tethering forces o
    Manfred Lindau; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Many cell types release molecules packaged in membrane bound secretory vesicles or granules by exocytosis...
  32. Human Eosinophils: Mechanisms of Functioning
    Peter F Weller; Fiscal Year: 2013
    ..1) piecemeal degranulation by which granule contents are mobilized into and secreted by cytoplasmic secretory vesicles, and 2) release of intact granules extruded by cytolysis from within eosinophils...
  33. Mechanism of transport of secretory vesicles in regulated secretory pathway
    JOSHUA JIN HYOUK PARK; Fiscal Year: 2012
    ..The candidate found that post-Golgi transport of newly synthesized BDNF/ACTH secretory vesicles from the cell body of neurons and endocrine cells to the release site at axonal/dendritic terminals or ..
  34. Structure and Function of the Exocyst Complex
    Mary Munson; Fiscal Year: 2013
    ..implicated in a number of different functions: selection of the site of exocytosis;physical tethering of secretory vesicles to these sites;communicating with cytoskeletal and cell cycle proteins;and regulating the specificity and ..
  35. Molecular mechanism of exocyst-centriolin complexes in abscission
    Heidi Hehnly; Fiscal Year: 2011
    ..Both endocytic and secretory vesicles are required for cell cleavage at the midbody via fusion to the plasma membrane and possibly each other...
  36. Characterization of Mitochondrial GTP as an Intramitochondrial Metabolic Signal
    Richard G Kibbey; Fiscal Year: 2012
    ..mtGTP is likely to be a very early signal dependent upon other mediators to transmit its signal to the secretory vesicles in the cytosol...
  37. Technologies to Define and Map Novel Interorganelle Macromolecular Interactions
    VLADISLAV VERKHUSHA; Fiscal Year: 2013
    ..for live imaging are compromised by the oxidizing environment of many organelles, including ER, Golgi, and secretory vesicles. The goal of this proposal is to define protein complexes that define and modulate novel organelle ..
  38. Human Eosinophils: Mechanisms of Functioning
    Peter Weller; Fiscal Year: 2009
    ..distinctly contain multiple preformed cytokines stored within morphologically unique granules and secretory vesicles. Eosinophils in tissue sites of inflammation, such as asthmatic airways, exhibit ultrastructural alterations ..
  39. AFM studies of SNARE-mediated membrane fusion
    Vincent T Moy; Fiscal Year: 2012
    ..Neurotransmitters (e.g., glutamate, GABA, catecholamine) within unstimulated neurons are sequestered within secretory vesicles docked at the plasma membrane of the presynaptic terminal...
  40. Characterization of zinc dysregulation in normal and cancerous prostate cells.
    JOSE GUADALUPE MIRANDA; Fiscal Year: 2012
    ..Cells have the ability to store labile Zn+2 in secretory vesicles and are able to mobilize this metal in response to certain cellular cues, such as stresses or cellular ..
  41. MINORITY PREDOCTORAL FELLOWSHIP PROGRAM
    FELIPE H SANTIAGO; Fiscal Year: 2010
    ..One of its cargoes is the secretory vesicles, that originate from the Golgi...
  42. Single Exo/Endocytic Events in Neurons &Endocrine Cells
    Wolfhard Almers; Fiscal Year: 2013
    ..of direct assay, it is still unknown how the proteins mentioned above interact with each other or with secretory vesicles in living cells...
  43. Identifying the Molecular Pathways Regulating Glucose-dependent Insulin Secretion
    David Altshuler; Fiscal Year: 2013
    ..we developed a high-throughput luminescent insulin secretion assay in which luciferase is targeted to the secretory vesicles of a beta cell an co-secreted with insulin upon stimulation...
  44. ROLE OF PEPTIDES IN OCULOMOTOR NEUROTRANSMISSION
    VINCENT CHIAPPINELLI; Fiscal Year: 1992
    ..These include some defects in accommodation, internal ophthalmoplegia and tonic pupil. In addition, the condition of the pupil is an important diagnostic sign in numerous ocular syndromes and in traumatic injuries to the head...
  45. SECRETION FROM ADIPOSE CELLS
    Konstantin V Kandror; Fiscal Year: 2010
    ..newly discovered Golgi-localized, gamma-ear-containing, Arf-binding proteins participate in the formation of secretory vesicles in adipocytes and regulate secretion from these cells...
  46. GENETICS OF SECRETION IN YEAST
    PETER JAY NOVICK; Fiscal Year: 2013
    ..on the vesicle surface as well as polarity determinants on the cell cortex and thus tethers incoming secretory vesicles to these specialized sites...
  47. Coordination of membrane traffic through rab GEF and GAP cascades
    PETER JAY NOVICK; Fiscal Year: 2013
    ..5. PI4P is normally restricted to the Golgi and is not enriched on Golgi-derived secretory vesicles. We will determine the mechanism by which PI4P is limited to the Golgi.
  48. The role of the exocyst in cell migration
    Wei Guo; Fiscal Year: 2013
    ..actions of the exocyst complex, the Arp2/3 complex, and the EHD family of "pinchases" in the generation of secretory vesicles from the sorting endosome that mediates integrins recycling to the leading edge for migration...
  49. BIOSYNTHESIS OF ENKEPHALIN OPIOID PEPTIDES
    Vivian Hook; Fiscal Year: 2009
    ..Cathepsin L is colocalized in secretory vesicles with enkephalin, and cathepsin L cleaves enkephalin-containing substrates at identical cleavage sites as ..
  50. Clinical Trial of DRibble Vaccine in NSCLC
    Walter Urba; Fiscal Year: 2009
    ..protein degradation causing highly immunogenic tumor antigens to accumulate as ubiquitinated proteins in secretory vesicles of treated cancer cells...
  51. In vivo Analysis of DISABLED Function and Interactions in Synaptic Vesicle Cyclin
    Richard W Ordway; Fiscal Year: 2013
    ..DAB) proteins in the synaptic vesicle endocytosis - the process which recycles neurotransmitter filled secretory vesicles after they fuse with the surface membrane and release their contents...
  52. Developmental Regulation of the Secretory Pathway in Yeast
    Aaron Neiman; Fiscal Year: 2009
    ..In this instance, retargetting of secretory vesicles gives rise to a new membrane compartment, the prospore membrane...
  53. Serpin Protease Inhibitors & Opioid Neuropeptides
    Vivian Hook; Fiscal Year: 2006
    ..PE) and proopiomelanocortin (POMC) precursors, respectively, which require proteolytic processing in secretory vesicles to form active neuropeptides...
  54. HETEROTRIMERIC G PROTEINS AND EXOCYTOSIS
    BHANU JENA; Fiscal Year: 1999
    ..In the last few years, several proteins associated with secretory vesicles have been identified and some characterized...
  55. Biogenesis of the Regulated Secretory Pathway
    Daniel W Sirkis; Fiscal Year: 2012
    ..of LDCVs, I am using the vesicular monoamine transporter (VMAT2), a polytopic membrane protein which fills secretory vesicles with monoamine neurotransmitters for exocytotic release...
  56. REGULATION OF ENDOCYTOSIS IN NEUROENDOCRINE CELLS
    Cristina Artalejo; Fiscal Year: 2004
    ..This project will enhance our understanding of vesicle retrieval that underlies vesicle recvclin2 processes in secretory cells. ..
  57. ELECTRON TRANSPORT ENZYMES IN CATECHOLAMINE GRANULES
    Patrick Fleming; Fiscal Year: 1990
    ..is to describe in molecular terms the structure and function of two oxidation/reduction enzymes in the secretory vesicles of neuroendocrine cells--dopamine beta-hydroxylase (DBH) and cytochrome b561...
  58. POMC Processing and Beta-Endorphine-Related Opioid Peptides
    Vivian Y H Hook; Fiscal Year: 2010
    ..These novel results were obtained by purification from secretory vesicles, active-site affinity labeling, and peptide microsequencing to identify the processing activity as cathepsin ..
  59. The exocyst and EHD proteins in endosome recycling and cell migration
    JOHN ANDREW SCHMIDT; Fiscal Year: 2012
    The exocyst is an octameric protein complex essential for exocytosis. While the exocyst functions to tether secretory vesicles at the plasma membrane for subsequent fusion, most of the exocyst components are localized to internal membrane ..
  60. GENETICS OF SECRETION IN YEAST
    Peter J Novick; Fiscal Year: 2010
    ..This proposal focuses on the exocyst complex, its assembly and its interactions with the secretory vesicles and plasma membrane...
  61. Inhibitors of beta-Amyloid Production in Alzheimer's
    Gregory Hook; Fiscal Year: 2007
    ..We purified beta-secretase activity from isolated regulated secretory vesicles and determined by microsequencing that the beta-secretase activity is due cathepsin B and cathepsin L...
  62. Stoichiometry and Architecture of the Vesicle Fusion Machine in PC-12 Cells
    JAMES WEISSHAAR; Fiscal Year: 2009
    ..The abrupt influx of Ca2+ triggers exocytosis, the process by which secretory vesicles docked at the PM open a fusion pore and spill their neurotransmitter into the synaptic cleft...
  63. REGULATION/INSULIN SECRETION BY PROTEIN PHOSPHORYLATION
    John Hutton; Fiscal Year: 2003
    ..Phogrin is of additional interest in type 1 diabetes as it is a major autoantigen. ..
  64. Drugs to Elevate Enkephalin as Novel Opiate Analgesics
    Terry Reisine; Fiscal Year: 2004
    ..We have identified the major proenkephalin cleaving activity in secretory vesicles for the generation of enkephalin, and it has been demonstrated as the cysteine protease cathepsin L...
  65. Sympathetic Outflow to Catecholamine Storage Vesicles
    DANIEL O CONNOR; Fiscal Year: 2006
    ..II [SgII], are a family of acidic proteins found in cores of amine and peptide hormone and neurotransmitter secretory vesicles, such as chromaffin granules and large dense core vesicles of post-ganglionic sympathetic (noradrenergic) ..