phagosomes

Summary

Summary: Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.

Top Publications

  1. ncbi Human IRGM induces autophagy to eliminate intracellular mycobacteria
    Sudha B Singh
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Science 313:1438-41. 2006
  2. pmc Methods in mammalian autophagy research
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Cell 140:313-26. 2010
  3. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
  4. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
  5. ncbi Autophagy: process and function
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Genes Dev 21:2861-73. 2007
  6. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
  7. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
  8. pmc Regulation mechanisms and signaling pathways of autophagy
    Congcong He
    Life Sciences Institute and Departments of Molecular, Cellular and Developmental Biology, and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Annu Rev Genet 43:67-93. 2009
  9. ncbi Autophagosome formation: core machinery and adaptations
    Zhiping Xie
    Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Nat Cell Biol 9:1102-9. 2007
  10. pmc Autophagy in health and disease: a double-edged sword
    Takahiro Shintani
    University of Michigan, Life Sciences Institute, Ann Arbor, MI 48109 2216, USA
    Science 306:990-5. 2004

Detail Information

Publications274 found, 100 shown here

  1. ncbi Human IRGM induces autophagy to eliminate intracellular mycobacteria
    Sudha B Singh
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Science 313:1438-41. 2006
    ..We also identified a function for a human IRG protein in the control of intracellular pathogens and report that the human Irgm1 ortholog, IRGM, plays a role in autophagy and in the reduction of intracellular bacillary load...
  2. pmc Methods in mammalian autophagy research
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Cell 140:313-26. 2010
    ..In this Primer, we discuss methods to monitor autophagy and to modulate autophagic activity, with a primary focus on mammalian macroautophagy...
  3. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
    ..The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes...
  4. pmc Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
    Autophagy 4:151-75. 2008
    ..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response...
  5. ncbi Autophagy: process and function
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Genes Dev 21:2861-73. 2007
    ..In this review, the process of autophagy is summarized, and the role of autophagy is discussed in a process-based manner...
  6. pmc In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker
    Noboru Mizushima
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Mol Biol Cell 15:1101-11. 2004
    ..Our results suggest that the regulation of autophagy is organ dependent and the role of autophagy is not restricted to the starvation response. This transgenic mouse model is a useful tool to study mammalian autophagy...
  7. ncbi Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Autophagy 3:452-60. 2007
    ..This method will be of general utility for analysis of the autophagosome maturation process...
  8. pmc Regulation mechanisms and signaling pathways of autophagy
    Congcong He
    Life Sciences Institute and Departments of Molecular, Cellular and Developmental Biology, and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    Annu Rev Genet 43:67-93. 2009
    ..We also review the recent advances on the molecular mechanisms that regulate the autophagy machinery at various levels, from transcriptional activation to post-translational protein modification...
  9. ncbi Autophagosome formation: core machinery and adaptations
    Zhiping Xie
    Life Sciences Institute and Department of Molecular, Cellular and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Nat Cell Biol 9:1102-9. 2007
    ..Additional components allow autophagy to adapt to the changing needs of the cell...
  10. pmc Autophagy in health and disease: a double-edged sword
    Takahiro Shintani
    University of Michigan, Life Sciences Institute, Ann Arbor, MI 48109 2216, USA
    Science 306:990-5. 2004
    ..By mostly morphological studies, autophagy has been linked to disease processes. Whether autophagy protects from or causes disease is unclear. Here, we summarize current knowledge about the role of autophagy in disease and health...
  11. pmc Network organization of the human autophagy system
    Christian Behrends
    Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 466:68-76. 2010
    ..These studies provide a global view of the mammalian autophagy interaction landscape and a resource for mechanistic analysis of this critical protein homeostasis pathway...
  12. ncbi The role of Atg proteins in autophagosome formation
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Annu Rev Cell Dev Biol 27:107-32. 2011
    ..In this review we describe the molecular mechanism of autophagosome formation with particular focus on the function of Atg proteins and the long-standing discussion regarding the origin of the autophagosome membrane...
  13. pmc Inhibition of macroautophagy triggers apoptosis
    Patricia Boya
    CNRS UMR 8125, Institut Gustave Roussy, Pavillon de Recherche 1, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Mol Cell Biol 25:1025-40. 2005
    ..All together, these data indicate that autophagy may be cytoprotective, at least under conditions of nutrient depletion, and point to an important cross talk between type 1 and type 2 cell death pathways...
  14. ncbi Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells
    A Yamamoto
    Department of Physiology and Liver Research Center Cell Biology, Kansai Medical University, Osaka, Japan
    Cell Struct Funct 23:33-42. 1998
    ..These results suggested that acidification of the lumenal space of autophagosomes or lysosomes by V-ATPase is important for the fusion between autophagosomes and lysosomes...
  15. ncbi Autophagy is a defense mechanism inhibiting BCG and Mycobacterium tuberculosis survival in infected macrophages
    Maximiliano G Gutierrez
    Instituto de Histologia y Embriologia, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza 5500, Argentina
    Cell 119:753-66. 2004
    Mycobacterium tuberculosis is an intracellular pathogen persisting within phagosomes through interference with phagolysosome biogenesis...
  16. ncbi A role for ubiquitin in selective autophagy
    Vladimir Kirkin
    Institute of Biochemistry II and Cluster of Excellence Macromolecular Complexes, Goethe University, Theodor Stern Kai 7, Frankfurt, Germany
    Mol Cell 34:259-69. 2009
    ..This review explores the hypothesis that ubiquitin represents a selective degradation signal suitable for targeting various types of cargo, ranging from protein aggregates to membrane-bound organelles and microbes...
  17. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
    ..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
  18. ncbi Autophagy: from phenomenology to molecular understanding in less than a decade
    Daniel J Klionsky
    Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109 2216, USA
    Nat Rev Mol Cell Biol 8:931-7. 2007
    ..Amazingly, the molecular breakthroughs in autophagy have taken place during only the past decade...
  19. pmc Autophagy as a regulated pathway of cellular degradation
    D J Klionsky
    Department of Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109 1048, USA
    Science 290:1717-21. 2000
    ..Macroautophagy plays an important role in developmental processes, human disease, and cellular response to nutrient deprivation...
  20. ncbi Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy
    Isei Tanida
    Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan
    Autophagy 1:84-91. 2005
    ....
  21. ncbi LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
    Yukiko Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Myodaiji 38, Okazaki 444 8585, Japan
    J Cell Sci 117:2805-12. 2004
    ..Generation of the form II correlates with autophagosome association of GABARAP and GATE16. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II...
  22. pmc Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 19:5360-72. 2008
    ..These results suggest that mammalian cells have at least two distinct class III PI3-kinase complexes, which may function in different membrane trafficking pathways...
  23. ncbi M. tuberculosis and M. leprae translocate from the phagolysosome to the cytosol in myeloid cells
    Nicole van der Wel
    The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 129:1287-98. 2007
    ..to be prototypical intracellular pathogens that have evolved strategies to enable growth in the intracellular phagosomes. In contrast, we show that lysosomes rapidly fuse with the virulent M. tuberculosis- and M...
  24. ncbi Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion
    Hitoshi Nakatogawa
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    Cell 130:165-78. 2007
    ..Our results provide further insights into the mechanisms underlying the unique membrane dynamics of autophagy and also indicate the functional versatility of ubiquitin-like proteins...
  25. ncbi Dynamics and diversity in autophagy mechanisms: lessons from yeast
    Hitoshi Nakatogawa
    Integrated Research Institute, Tokyo Institute of Technology, Yokohama 2268503, Japan
    Nat Rev Mol Cell Biol 10:458-67. 2009
    ..Analyses of autophagy-related (Atg) proteins have unveiled dynamic and diverse aspects of mechanisms that underlie membrane formation during autophagy...
  26. ncbi A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation
    Mitsuko Hayashi-Nishino
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nat Cell Biol 11:1433-7. 2009
    ..Electron tomography revealed that the ER-IM complex appears as a subdomain of the ER that formed a cradle encircling the IM, and showed that both ER and isolation membranes are interconnected...
  27. ncbi 3D tomography reveals connections between the phagophore and endoplasmic reticulum
    Päivi Ylä-Anttila
    University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, Helsinki, Finland
    Autophagy 5:1180-5. 2009
    ..This finding has potential implications on the origin of autophagosome membranes, and on the mechanism of phagophore membrane extension. In addition, we observed lipid droplets in very close contact with the phagophores/autophagosomes...
  28. ncbi Discovery of Atg5/Atg7-independent alternative macroautophagy
    Yuya Nishida
    Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8510, Japan
    Nature 461:654-8. 2009
    ..These results indicate that mammalian macroautophagy can occur through at least two different pathways: an Atg5/Atg7-dependent conventional pathway and an Atg5/Atg7-independent alternative pathway...
  29. pmc Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Bunkyo ku, Tokyo, Japan
    Autophagy 6:764-76. 2010
    ..These hierarchical analyses suggest that ULK1, Atg14 and VMP1 localize to the ER-associated autophagosome formation sites in a PI3-kinase activity-independent manner...
  30. ncbi Does bafilomycin A1 block the fusion of autophagosomes with lysosomes?
    Daniel J Klionsky
    Autophagy 4:849-950. 2008
    ..However, data from one of our labs noted an apparently different result in a relatively recent manuscript. Therefore, we decided to look into this more carefully...
  31. pmc Plasma membrane contributes to the formation of pre-autophagosomal structures
    Brinda Ravikumar
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge, CB2 2XY, UK
    Nat Cell Biol 12:747-57. 2010
    ....
  32. ncbi Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages
    Kohichi Matsunaga
    Department of Cellular Regulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Nat Cell Biol 11:385-96. 2009
    ..These data suggest that the Beclin 1-hVps34 complex functions in two different steps of autophagy by altering the subunit composition...
  33. pmc HDAC6 controls autophagosome maturation essential for ubiquitin-selective quality-control autophagy
    Joo Yong Lee
    Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
    EMBO J 29:969-80. 2010
    ..Our study identifies HDAC6 and the actin cytoskeleton as critical components that define QC autophagy and uncovers a novel regulation of autophagy at the level of autophagosome-lysosome fusion...
  34. pmc Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
    N Mizushima
    Unit Process and Combined Circuit, PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Biol 152:657-68. 2001
    ..We also show that Apg12-Apg5 is required for targeting of a mammalian Aut7/Apg8 homologue, LC3, to the isolation membranes. These results suggest that the Apg12-Apg5 conjugate plays essential roles in isolation membrane development...
  35. ncbi Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis
    Miguel A Sanjuan
    Department of Immunology, St Jude Children s Research Institute, Memphis, Tennessee 38105, USA
    Nature 450:1253-7. 2007
    ..Therefore we speculated that TLR signalling might link these processes to enhance the function of conventional phagosomes. Here we show that a particle that engages TLRs on a murine macrophage while it is phagocytosed triggers the ..
  36. pmc Caspase-11 promotes the fusion of phagosomes harboring pathogenic bacteria with lysosomes by modulating actin polymerization
    Anwari Akhter
    Department of Microbial Infection and Immunity, Center for Microbial Interface Biology, The Ohio State University, Columbus, OH 43210, USA
    Immunity 37:35-47. 2012
    ..However, caspase-11 was dispensable for the fusion of lysosomes with phagosomes containing nonpathogenic bacteria, uncovering a fundamental difference in the trafficking of phagosomes ..
  37. pmc Microtubule-associated protein 1 light chain 3 alpha (LC3)-associated phagocytosis is required for the efficient clearance of dead cells
    Jennifer Martinez
    Department of Immunology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Proc Natl Acad Sci U S A 108:17396-401. 2011
    ..Recently, a process was identified wherein the autophagy machinery is recruited to pathogen-containing phagosomes, termed MAPLC3A (LC3)-associated phagocytosis (LAP), which results in optimal degradation of the phagocytosed ..
  38. pmc Antigen-loading compartments for major histocompatibility complex class II molecules continuously receive input from autophagosomes
    Dorothee Schmid
    Laboratory of Viral Immunobiology and Christopher H Browne Center for Immunology and Immune Diseases, The Rockefeller University, New York, NY 10021, USA
    Immunity 26:79-92. 2007
    ..We suggest that macroautophagy constitutively and efficiently delivers cytosolic proteins for MHC class II presentation and can be harnessed for improved helper T cell stimulation...
  39. ncbi HSV-1 ICP34.5 confers neurovirulence by targeting the Beclin 1 autophagy protein
    Anthony Orvedahl
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell Host Microbe 1:23-35. 2007
    ..Our findings suggest that autophagy inhibition is a novel molecular mechanism by which viruses evade innate immunity and cause fatal disease...
  40. ncbi The cell biology of phagocytosis
    Ronald S Flannagan
    Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Annu Rev Pathol 7:61-98. 2012
    ..We discuss the manner in which phagocytosis is subverted by certain pathogens and consider congenital disorders that affect phagocyte function...
  41. ncbi Antimicrobial mechanisms of phagocytes and bacterial evasion strategies
    Ronald S Flannagan
    Program in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada
    Nat Rev Microbiol 7:355-66. 2009
    ..In addition, using Mycobacterium tuberculosis, Listeria monocytogenes, Legionella pneumophila and Coxiella burnetii as examples, we describe some of the evasive strategies used by bacteria...
  42. pmc A role for phosphoinositide 3-kinase in the completion of macropinocytosis and phagocytosis by macrophages
    N Araki
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 135:1249-60. 1996
    ..of actin-rich pseudopodia along IgG-opsonized sheep erythrocytes, but instead prevented them from closing into phagosomes. These findings indicate that PI 3-kinase is not necessary for receptor-mediated stimulation of pseudopod ..
  43. pmc Formation process of autophagosome is traced with Apg8/Aut7p in yeast
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 147:435-46. 1999
    ..Moreover, it is revealed that autophagosome formation is severely impaired in the apg8 null mutant. Apg8p would play an important role in the autophagosome formation...
  44. ncbi Autophagosome formation and molecular mechanism of autophagy
    Isei Tanida
    Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Shinjyuku, Japan
    Antioxid Redox Signal 14:2201-14. 2011
    ..Processes of selective autophagy require specific Atg proteins in addition to the "core" Atg complexes. Finally, methods to monitor autophagic activity in mammalian cells are described...
  45. pmc Subversion of cellular autophagosomal machinery by RNA viruses
    William T Jackson
    Department of Microbiology, Stanford University, Stanford, California, USA
    PLoS Biol 3:e156. 2005
    ..Instead, we argue that these double-membraned structures provide membranous supports for viral RNA replication complexes, possibly enabling the nonlytic release of cytoplasmic contents, including progeny virions, from infected cells...
  46. pmc SNARE proteins are required for macroautophagy
    Usha Nair
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Cell 146:290-302. 2011
    ..Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis...
  47. pmc Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection
    Luc English
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Succursale Centre Ville, Montreal, Quebec, Canada
    Nat Immunol 10:480-7. 2009
    ..Viral peptides in autophagosomes were further processed by the proteasome, which suggests a complex interaction between the vacuolar and MHC class I presentation pathways...
  48. ncbi C. elegans screen identifies autophagy genes specific to multicellular organisms
    Ye Tian
    National Institute of Biological Sciences, Beijing 102206, P R China
    Cell 141:1042-55. 2010
    ..This study establishes C. elegans as a multicellular genetic model to delineate the autophagy pathway and provides mechanistic insights into the metazoan-specific autophagic process...
  49. ncbi NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells
    Ariel Savina
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Cell 126:205-18. 2006
    ..The NADPH oxidase NOX2 is recruited to the DC's early phagosomes and mediates the sustained production of low levels of reactive oxygen species, causing active and maintained ..
  50. ncbi Killing activity of neutrophils is mediated through activation of proteases by K+ flux
    Emer P Reeves
    Centre for Molecular Medicine, University College London, 5 University Street, London WC1E 6JJ, UK
    Nature 416:291-7. 2002
    ..We show that it is the proteases, thus activated, that are primarily responsible for the destruction of the bacteria...
  51. pmc An Atg9-containing compartment that functions in the early steps of autophagosome biogenesis
    Muriel Mari
    Department of Cell Biology and Institute of Biomembranes, University Medical Centre Utrecht, Utrecht University, 3584 CX Utrecht, The Netherlands
    J Cell Biol 190:1005-22. 2010
    ..Thus, our data reveal that the Atg9-positive compartments are important for the de novo formation of the PAS and the sequestering vesicle that are the hallmarks of autophagy...
  52. ncbi A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes
    Hiroki Nagai
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    Science 295:679-82. 2002
    ..The RalF protein is required for the localization of ARF on phagosomes containing L. pneumophila...
  53. ncbi The Toll-like receptor 2 is recruited to macrophage phagosomes and discriminates between pathogens
    D M Underhill
    Department of Immunology, University of Washington, Seattle 98195, USA
    Nature 401:811-5. 1999
    ..Here we show that Toll-like receptor 2 is recruited specifically to macrophage phagosomes containing yeast, and that a point mutation in the receptor abrogates inflammatory responses to yeast and Gram-..
  54. ncbi Cell biology of mycobacterium tuberculosis phagosome
    Isabelle Vergne
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131 001, USA
    Annu Rev Cell Dev Biol 20:367-94. 2004
    ..Here we review the fundamental trafficking processes targeted by M. tuberculosis and the mycobacterial products that interfere with phagosomal maturation...
  55. pmc Activation of antibacterial autophagy by NADPH oxidases
    Ju Huang
    Cell Biology Program, Hospital for Sick Children, Toronto, ON, Canada M5G 1X8
    Proc Natl Acad Sci U S A 106:6226-31. 2009
    Autophagy plays an important role in immunity to microbial pathogens. The autophagy system can target bacteria in phagosomes, promoting phagosome maturation and preventing pathogen escape into the cytosol...
  56. pmc Mitochondria supply membranes for autophagosome biogenesis during starvation
    Dale W Hailey
    The Eunice Kennedy Shriver National Institutes of Child Health and Human Development, 18 Library Drive, Bethesda, MD 20892, USA
    Cell 141:656-67. 2010
    ..Disruption of mitochondria/ER connections by mitofusin2 depletion dramatically impairs starvation-induced autophagy. Mitochondria thus play a central role in starvation-induced autophagy, contributing membrane to autophagosomes...
  57. ncbi Maturation of autophagic vacuoles in Mammalian cells
    Eeva Liisa Eskelinen
    Institute of Biochemistry, University of Kiel, Germany
    Autophagy 1:1-10. 2005
    ..This suggests that autophagy has important housekeeping or protective functions because a block in autophagic maturation causes a disease...
  58. pmc Atg8 controls phagophore expansion during autophagosome formation
    Zhiping Xie
    Life Sciences Institute and Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:3290-8. 2008
    ..This model provides a foundation for future analyses of the functions and dynamics of known autophagy-related proteins and for screening new genes...
  59. pmc The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells
    Zhen Lu
    Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
    J Clin Invest 118:3917-29. 2008
    ..Thus, ARHI can induce autophagic cell death, but can also promote tumor dormancy in the presence of factors that promote survival in the cancer microenvironment...
  60. pmc Mycobacterium tuberculosis prevents inflammasome activation
    Sharon S Master
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA
    Cell Host Microbe 3:224-32. 2008
    ..activation of the inflammasome, resulting in increased IL-1beta secretion, enhanced maturation of Mtb containing phagosomes, improved mycobacterial clearance by macrophages, and lower bacterial burden in the lungs of aerosol-infected ..
  61. pmc Autophagosome supports coxsackievirus B3 replication in host cells
    Jerry Wong
    The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, University of British Columbia St Paul s Hospital, 1081 Burrard St, Vancouver, British Columbia, Canada
    J Virol 82:9143-53. 2008
    ..Taken together, our results suggest that the host's autophagy machinery is activated during CVB3 infection to enhance the efficiency of viral replication...
  62. pmc Autophagosome precursor maturation requires homotypic fusion
    Kevin Moreau
    Department of Medical Genetics, Cambridge Institute for Medical Research, Wellcome MRC Building, Addenbrooke s Hospital, Hills Road, Cambridge CB2 20Y, UK
    Cell 146:303-17. 2011
    ....
  63. ncbi ESCRT-III dysfunction causes autophagosome accumulation and neurodegeneration
    Jin A Lee
    Gladstone Institute of Neurological Disease and Department of Neurology, University of California, San Francisco, California 94158, USA
    Curr Biol 17:1561-7. 2007
    ....
  64. pmc Phagosome maturation: going through the acid test
    Jason M Kinchen
    Beirne B Carter Center for Immunology Research and the Department of Microbiology, University of Virginia, Charlottesville, Virginia 22902, USA
    Nat Rev Mol Cell Biol 9:781-95. 2008
    ..have led to the identification of numerous candidate proteins that cooperate to control the maturation of phagosomes containing different particles...
  65. ncbi Endogenous MHC class II processing of a viral nuclear antigen after autophagy
    Casper Paludan
    Laboratory of Viral Immunobiology, Rockefeller University, New York, NY 10021, USA
    Science 307:593-6. 2005
    ..Thus, lysosomal processing after autophagy may contribute to MHC class II-restricted surveillance of long-lived endogenous antigens including nuclear proteins relevant to disease...
  66. pmc Cdc42, Rac1, and Rac2 display distinct patterns of activation during phagocytosis
    Adam D Hoppe
    Department of Microbiology and Immunology and the Biophysics Research Division, University of Michigan Medical School, Ann Arbor, Michigan 48109 0620, USA
    Mol Biol Cell 15:3509-19. 2004
    ..resonance energy transfer stoichiometry allowed calculation of the fractions of activated GTPases in forming phagosomes. Cdc42 activation was restricted to the leading margin of the cell, whereas Rac1 was active throughout the ..
  67. pmc Role of phosphatidylinositol 3-kinase and Rab5 effectors in phagosomal biogenesis and mycobacterial phagosome maturation arrest
    R A Fratti
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Cell Biol 154:631-44. 2001
    ..The precise mechanisms governing maturation of phagosomes into phagolysosomes are not completely understood...
  68. pmc The role of autophagy in mammalian development: cell makeover rather than cell death
    Francesco Cecconi
    Dulbecco Telethon Institute at the Department of Biology, University of Rome Tor Vergata, 00133 Rome, Italy
    Dev Cell 15:344-57. 2008
    ..Thus, autophagy is a process of cytosolic "renovation," crucial in cell fate decisions...
  69. ncbi The phagosomal proteome in interferon-gamma-activated macrophages
    Matthias Trost
    Department of Pathology and Cell Biology, Universite de Montreal, Succ Centre Ville, Montreal, Quebec, Canada
    Immunity 30:143-54. 2009
    ....
  70. pmc LAMP proteins are required for fusion of lysosomes with phagosomes
    Kassidy K Huynh
    Division of Cell Biology, The Hospital for Sick Children, Toronto, Canada
    EMBO J 26:313-24. 2007
    Lysosome-associated membrane proteins 1 and 2 (LAMP-1 and LAMP-2) are delivered to phagosomes during the maturation process. We used cells from LAMP-deficient mice to analyze the role of these proteins in phagosome maturation...
  71. ncbi Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes
    Robert Köchl
    Cancer Research UK, London Research Institute, Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Traffic 7:129-45. 2006
    ..Stimulation of autophagosome formation by vinblastine was independent of nutrients and mTOR activity but was inhibited by depletion of the Autophagy proteins Atg5 and Atg6, known to be required for autophagy...
  72. ncbi Shigella phagocytic vacuolar membrane remnants participate in the cellular response to pathogen invasion and are regulated by autophagy
    Nicolas Dupont
    Cellular Microbiology and Infectious Pathogeny UMR8161 CNRS, 1 rue du Prof Calmette, F 59021 Lille, France
    Cell Host Microbe 6:137-49. 2009
    ....
  73. ncbi Escape of intracellular Shigella from autophagy
    Michinaga Ogawa
    Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1, Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Science 307:727-31. 2005
    ..Rather, Shigella VirG, a protein required for intracellular actin-based motility, induced autophagy by binding to the autophagy protein, Atg5. In nonmutant Shigella, this binding is competitively inhibited by IcsB binding to VirG...
  74. ncbi Mycobacterium tuberculosis and the environment within the phagosome
    Kyle Rohde
    Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
    Immunol Rev 219:37-54. 2007
    ..These approaches represent our ongoing attempts to unravel the discourse that takes place between the pathogen and its host cell...
  75. ncbi A phagosome of one's own: a microbial guide to life in the macrophage
    Amal O Amer
    University of Michigan Medical School, 6734 Medical Sciences Building II, Ann Arbor, MI 48109 0620, USA
    Curr Opin Microbiol 5:56-61. 2002
    Macrophages protect their host by engulfing foreign bodies within phagosomes that rapidly develop into microbicidal organelles...
  76. ncbi Immune control of tuberculosis by IFN-gamma-inducible LRG-47
    John D MacMicking
    Laboratory of Infection Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 302:654-9. 2003
    ..killing in IFN-gamma-activated LRG-47-/- macrophages was associated with impaired maturation of Mtb-containing phagosomes, vesicles that otherwise recruited LRG-47 in wild-type cells...
  77. pmc Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis
    Yoshinori Takahashi
    H Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive Tampa, FL 33612, USA
    Nat Cell Biol 9:1142-51. 2007
    ..Moreover, knockout of Bif-1 significantly enhances the development of spontaneous tumours in mice. These findings suggest that Bif-1 joins the UVRAG-Beclin 1 complex as a potential activator of autophagy and tumour suppressor...
  78. pmc Autophagy requires endoplasmic reticulum targeting of the PI3-kinase complex via Atg14L
    Kohichi Matsunaga
    Department of Genetics, Graduate School of Medicine, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    J Cell Biol 190:511-21. 2010
    ..The Atg14L-dependent appearance of PI3P in the ER makes this organelle the platform for autophagosome formation...
  79. pmc A mammalian autophagosome maturation mechanism mediated by TECPR1 and the Atg12-Atg5 conjugate
    Dandan Chen
    Division of Biochemistry, Biophysics, and Structural Biology, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 45:629-41. 2012
    ....
  80. pmc Replication of hepatitis C virus RNA on autophagosomal membranes
    Donna Sir
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 287:18036-43. 2012
    ..These results indicate that HCV induces autophagosomes via a Class III PI3K-independent pathway and uses autophagosomal membranes as sites for its RNA replication...
  81. pmc COP9 signalosome regulates autophagosome maturation
    Huabo Su
    Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, 414 E Clark Street, Vermillion, SD 57069, USA
    Circulation 124:2117-28. 2011
    ..The COP9 signalosome (CSN) is a conserved protein complex consisting of 8 subunits (CSN1 through CSN8), and is known to regulate the ubiquitin-proteasome system. However, it is unknown whether CSN plays a role in autophagy...
  82. pmc Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets
    Anoop Kumar G Velikkakath
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Mol Biol Cell 23:896-909. 2012
    ..These data suggest that mammalian Atg2 proteins function both in autophagosome formation and regulation of lipid droplet morphology and dispersion...
  83. pmc Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification
    Dennis Wong
    Department of Medicine, Division of Infectious Diseases, University of British Columbia, Vancouver, BC, Canada V5Z 3J5
    Proc Natl Acad Sci U S A 108:19371-6. 2011
    ..These findings show that inhibition of phagosome acidification in the mycobacterial phagosome is directly attributed to PtpA, a key protein needed for Mtb survival and pathogenicity within host macrophages...
  84. ncbi Modulation of lipid droplets by Mycobacterium leprae in Schwann cells: a putative mechanism for host lipid acquisition and bacterial survival in phagosomes
    Katherine A Mattos
    Laboratório de Microbiologia Celular, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio de Janeiro, RJ, Brazil
    Cell Microbiol 13:259-73. 2011
    ..Moreover, via confocal and live-cell analysis, it was found that LDs are promptly recruited to bacterial phagosomes and that this process depends on cytoskeletal reorganization and PI3K signalling...
  85. pmc VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD
    Emilie Tresse
    Department of Developmental Neurobiology, St Jude Children s Research Hospital, Memphis, TN, USA
    Autophagy 6:217-27. 2010
    ..We conclude that VCP is essential for maturation of ubiquitin-containing autophagosomes and that defect in this function may contribute to IBMPFD pathogenesis...
  86. ncbi Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Struct Funct 33:109-22. 2008
    ..Collectively, our data demonstrate the functional significance of autophagosome movement that enables effective delivery from the cytosol to lysosomes...
  87. ncbi Mycobacterium tuberculosis: here today, and here tomorrow
    D G Russell
    Microbiology and Immunology, 5173 Veterinary Medical Center, Cornell University, Ithaca, New York 14853, USA
    Nat Rev Mol Cell Biol 2:569-77. 2001
    ..As the macrophage is key to clearing the infection, the interplay between the pathogen and its host cell reflects a constant battle for control...
  88. ncbi Transport of autophagosomes in neurites of PC12 cells during serum deprivation
    Yi Yang
    Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, P R China
    Autophagy 4:243-5. 2008
    ..These results provide valuable insight into neuritic transport of autophagosomes and imply a close relationship between the autophagic process and neurite degeneration...
  89. pmc The autophagy machinery is required to initiate hepatitis C virus replication
    Marlene Dreux
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 106:14046-51. 2009
    ....
  90. pmc Membrane delivery to the yeast autophagosome from the Golgi-endosomal system
    Yohei Ohashi
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Mol Biol Cell 21:3998-4008. 2010
    ..This is underestimated by examination of single mutants, providing a possible explanation for discrepancies between yeast and mammalian studies on Atg9 localization and autophagosome formation...
  91. ncbi Phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of RhoGEF/DH and FYVE domains in the parasitic protozoon Entamoeba histolytica
    Kumiko Nakada-Tsukui
    Department of Parasitology, National Institute of Infectious Diseases, Shinjuku ku, Tokyo, Japan
    Cell Microbiol 11:1471-91. 2009
    ..translocated to the phagocytic cup, phagosome, and to tunnel-like structures connecting the plasma membrane and phagosomes. E...
  92. ncbi Autophagy: a lysosomal degradation pathway with a central role in health and disease
    Eeva Liisa Eskelinen
    Department of Biological and Environmental Sciences, Division of Biochemistry, University of Helsinki, PO Box 56, FIN 00014 Helsinki, Finland
    Biochim Biophys Acta 1793:664-73. 2009
    ..In addition, impaired autophagic degradation contributes to the pathogenesis of several human diseases including lysosomal storage disorders and muscle diseases...
  93. pmc Proteasome inhibitors activate autophagy as a cytoprotective response in human prostate cancer cells
    K Zhu
    Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA
    Oncogene 29:451-62. 2010
    ..Overall, our data show that proteasome inhibition activates autophagy through a phospho-eIF2alpha-dependent mechanism to eliminate protein aggregates and alleviate proteotoxic stress...
  94. pmc Mechanism of phagolysosome biogenesis block by viable Mycobacterium tuberculosis
    Isabelle Vergne
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Proc Natl Acad Sci U S A 102:4033-8. 2005
    Live Mycobacterium tuberculosis persists in macrophage phagosomes by interfering with phagolysosome biogenesis...
  95. pmc Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties
    Marisa Ponpuak
    Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
    Immunity 32:329-41. 2010
    ..tuberculosis. Thus, p62 brings cytosolic proteins to autolysosomes where they are processed from innocuous precursors into neo-antimicrobial peptides, explaining in part the unique bactericidal properties of autophagic organelles...
  96. pmc Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae
    Aniek van der Vaart
    Department of Cell Biology and Institute of Biomembranes, University Medical Center Utrecht, Utrecht 3584, The Netherlands
    Mol Biol Cell 21:2270-84. 2010
    ..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism...
  97. ncbi The mitochondrial permeability transition initiates autophagy in rat hepatocytes
    S P Elmore
    Department of Cell and Developmental Biology and Curriculum in Toxicology, University of North Carolina, Chapel Hill, NC 27599, USA
    FASEB J 15:2286-7. 2001
    ..In conclusion, the MPT initiates mitochondrial depolarization after autophagic stimulation and the subsequent sequestration of mitochondria into autophagosomes...
  98. ncbi Conjugative transfer by the virulence system of Legionella pneumophila
    J P Vogel
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA
    Science 279:873-6. 1998
    ..The dot virulence genes encoded a large putative membrane complex that functioned as a secretion system that was able to transfer plasmid DNA from one cell to another...
  99. pmc The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella
    Shun Kageyama
    Laboratory of Intracellular Membrane Dynamics, Graduate School of Frontier Biosciences, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    Mol Biol Cell 22:2290-300. 2011
    ..We propose that the LC3 system is recruited through a mechanism that is independent of isolation membrane generation...
  100. ncbi A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation
    D B Munafó
    Laboratorio de Biología Celular y Molecular Instituto de Histología y Embriología, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo CONICET, Mendoza, 5500, Argentina
    J Cell Sci 114:3619-29. 2001
    ..Furthermore, these MDC-vacuoles were labeled with LC3, one of the mammalian homologues of the yeast protein Apg8/Aut7 that plays an important role in autophagosome formation...
  101. ncbi Mycobacterium tuberculosis inhibition of phagolysosome biogenesis and autophagy as a host defence mechanism
    Vojo Deretic
    Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, 915 Camino de Salud, NE Albuquerque, NM 87131 001, USA
    Cell Microbiol 8:719-27. 2006
    ..Mycobacteria prevent Rab conversion on their phagosomes and elaborate glycolipid and protein trafficking toxins that interfere with Rab effectors and regulation of ..

Research Grants63

  1. Human neutrophils, phospholipase A2 and S.aureus: microbial targets and responses
    William M Nauseef; Fiscal Year: 2013
    ..the cornerstone of cell-mediated antimicrobial activity and exert ~ all of their antimicrobial effort within phagosomes, where reactive oxygen species (ROS) and granule contents collaborate to kill and degrade microbes...
  2. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    ..Notably, LROs in DCs have been implicated in several functions, including fusion with phagosomes to neutralize phagosomal pH and thereby promote antigen survival to allow for escape and presentation by MHC-I...
  3. Phagosomal Signals Shape Inflammatory Responses to Borrelia Burgdorferi
    Juan C Salazar; Fiscal Year: 2010
    ..central hypothesis: "Phagocytosis of Borrelia burgdorferi and degradation of Lyme disease spirochetes within phagosomes induces diverse pro- and anti-inflammatory signaling cascades in monocytes/macrophages through a cooperative ..
  4. Role of Protein Kinase C in Macrophage Activation
    Michelle R Lennartz; Fiscal Year: 2012
    ..The mechanism of PKC-e action requires localization to phagosomes and catalytic activity...
  5. Role of Ion Channel in Mononuclear Phagocyte Activation
    Deborah J Nelson; Fiscal Year: 2012
    ..In order to perform this function, these cell types must ingest and destroy pathogens, generally in phagosomes, as well as secrete a number of products that signal other immune cells to respond...
  6. Vitamin D Inhibition of HIV and M. Tuberculosis Coinfection in Macrophages
    Stephen A Spector; Fiscal Year: 2010
    ..Mtb is an intracellular pathogen that persists within macrophage phagosomes through interference with phagolysosome biogenesis...
  7. Quorum sensing-dependent interactions with biofilms and innate immunity defenses
    Alexander R Horswill; Fiscal Year: 2012
    ..aureus cells that have detached from airway epithelia or escaped phagosomes. In each specific aim, we will also compare the transition mechanisms of laboratory strains with the community-..
  8. Novel mycobacterial translocase I inhibitors - a new class of anti-TB drugs
    Venkata M Reddy; Fiscal Year: 2010
    ..These particles were easily phagocytosed by macrophages and SQ641 was released inside phagosomes to efficiently and effectively kill intracellular MTB...
  9. Role of fascin1 in dendritic cell-mediated immunity against listeria infection
    Shigeko Yamashiro; Fiscal Year: 2013
    ..We further found that fascin1 made Lm-encapsulated phagosomes more acidic, and increases autophagy flux...
  10. The role of PhoP/PhoQ-regulated phospholipid alterations in Salmonella pathogenes
    ZACHARY DALEBROUX; Fiscal Year: 2013
    ..In Salmonella, LPS modification requires the highly conserved PhoP/Q two-component system. Within macrophage phagosomes, the membrane bound PhoQ sensor kinase is activated by low pH and AMP...
  11. Methods to enable cholesterol catabolism in human monocyte derived macrophages
    Richard E Honkanen; Fiscal Year: 2013
    ..Mycobacteria feed on cholesterol while contained in the phagosomes of macrophages. Importantly, two enzymes that catalyze cholestane ring opening have been identified...
  12. Innate Immune Sensing of Bacterial Sugars
    David M Underhill; Fiscal Year: 2013
    ..We have shown that upon degradation of peptidoglycan in phagosomes a component sugar, N-acetylglucosamine, is freed up and transported into the cytosol where it interacts with ..
  13. Functional Role of the Tetraspanin CD82/Kai1 in Innate and Adaptive Immunity
    Jatin M Vyas; Fiscal Year: 2013
    ..from CD82-/- DCs but other TLR signaling pathways remained intact 3) CD82 is recruited to phagosomes containing fungal pathogens including Aspergillus fumigatus as determined by time-lapse imaging of a ..
  14. Study of LY108 innate and adaptive immune responses
    CORNELIS P TERHORST; Fiscal Year: 2013
    ..iii) Ly108 modulates responses to bacteria by macrophages or neutrophils, most likely because Ly108 enters phagosomes as a "scavenger-receptor"...
  15. Mechanisms and regulation of macropinosome closure
    Joel A Swanson; Fiscal Year: 2010
    ..these studies will create a framework for analyzing molecular mechanisms of closure in macropinosomes and phagosomes. PUBLIC HEALTH RELEVANCE: Pinocytosis is a microscopic drinking activity, present in nearly all living cells, ..
  16. The role of TLR9 on Aspergillus fumigatus phagosomes
    Jatin M Vyas; Fiscal Year: 2013
    ..fumigatus phagosomes in macrophages results in a dramatic change of the subcellular distribution of TLR9 from the ER to a bright, ..
  17. Zeiss Laser TIRF 3 microscope and live cell imaging system
    Joseph E Mazurkiewicz; Fiscal Year: 2010
    ..assembly of the phagocytic cup as vesicles bringing new membrane to the assembly site, approach and fuse into phagosomes;the interaction of signaling molecules and the actin cytoskeleton during phagocytosis by macrophages as detected ..
  18. Role of caspases in Legionella pneumophila pulmonary infection
    Amal O Amer; Fiscal Year: 2013
    ..the design of new molecules that can specifically target particular caspases and manipulate the maturation of phagosomes containing intracellular lung pathogens...
  19. Mycobacterial Genes Responsible for Regulating Autophagy
    Sunhee Lee; Fiscal Year: 2012
    ..It has been demonstrated that stimulation of autophagic pathways in macrophages causes mycobacterial phagosomes to mature into phagolysosomes, which can overcome the trafficking block imposed by Mycobacterium tuberculosis...
  20. The Regulatory Control Mechanisms of Cross-presentation by Toll-like Receptors
    JULIE MAGARIAN BLANDER; Fiscal Year: 2012
    ..endocytic compartments is induced by TLR signals, ii) MHC class II presentation is controlled by TLRs, and iii) phagosomes are autonomous organelles that individually regulate MHC class II presentation depending on the nature and ..
  21. 2013 Phagocytes Gordon Research Conference and Seminar
    Lee Ann H Allen; Fiscal Year: 2013
    ..arthritis, and Alzheimer's disease, including recently identified therapeutic targets, and the use of phagosomes as tools for vaccinology...
  22. Regulation of Morphogenesis in C. albicans
    James B Konopka; Fiscal Year: 2013
    ..cells are >1,000-fold sensitive to copper, which correlates with decreased growth in macrophage phagosomes that are enriched in copper...
  23. Modes of cell death initiated by photodynamic therapy
    David Kessel; Fiscal Year: 2013
    ..Protection from photokilling can occur even when autophagy is incomplete, i.e., without the fusion of auto- phagosomes with lysosomes followed by proteolysis of the encapsulated cargo...
  24. pH Homeostasis in Mycobacterium tuberculosis
    Sabine Ehrt; Fiscal Year: 2013
    ..Mtb encounters an acidic pH within phagosomes of interferon-3 activated macrophages and must prevent excessive entry of protons into its cytosol...
  25. Pathways of Antigen Presentation by CD1
    Michael B Brenner; Fiscal Year: 2013
    ..We will then examine the role of Arl8b in the lysosomal trafficking necessary for delivery of its content to phagosomes in phago-lysosome fusion (Aim 4)...
  26. How bacteria corrupt the host vesicular trafficking:Role of SNARE-like Proteins
    FABIENNE MICHELLE PAUMET; Fiscal Year: 2012
    ..the bacterial SNARE-like domains should revert the blockage of fusion and allow bacteria clearance by the phagosomes. PUBLIC HEALTH RELEVANCE: This research project proposes to determine whether intracellular bacteria utilize ..
  27. M. avium GPLs in macrophage activation and virulence
    JEFFREY SCOTT SCHOREY; Fiscal Year: 2013
    ..receptor and that this interaction is required for the delayed accusation of lysosomal markers observed for phagosomes containing GPL-coated beads. Based on these observations we hypothesize that M...
  28. Mtb uptake and antigen presentation in human lung epithelial cells
    Melanie J Harriff; Fiscal Year: 2013
    ..Our hypothesis is that Mtb is taken up by epithelial cells into compartments that are distinct from phagosomes of professional antigen presenting cells, and that trafficking of and presentation of antigen on MR1 are ..
  29. Survival of Neisseria gonorrhoeae after primary human neutrophil challenge
    Alison K Criss; Fiscal Year: 2013
    ..Aim 1 will investigate the release of active APs from granules into phagosomes or at the PMN surface after infection with Gc, and how modulating AP release affects the survival of Gc inside ..
  30. How cell death and sterile particulates stimulate inflammation and disease
    Kenneth L Rock; Fiscal Year: 2013
    ..Our underlying hypotheses are that: (i) These particulate stimuli must be internalized into phagosomes of macrophages;(ii) After internalization the key event that leads to macrophage activation is rupture of some ..
  31. Modulation of Host Vesicle Trafficking by Legionella pneumophila
    Zhao Qing Luo; Fiscal Year: 2013
    ..SidJ, by coordinating with other effectors, redirects the trafficking of COPII-coated vesicles to the bacterial phagosomes. The goal of this project is to characterize SidJ and its protein phosphatase activity in Legionella infection ..
  32. Kim-1 regulation of autophagy and MHC presentation in kidney injury
    CRAIG ROBERT BROOKS; Fiscal Year: 2012
    ..Using chemical and genetic methods we will examine the trafficking of Kim-1 positive phagosomes to the autophagosome...
  33. Innate immune defense against community associated methicillin-resistant S.aureus
    William M Nauseef; Fiscal Year: 2013
    ..Aim 1 Determine the mechanisms and consequences of survival of SA in human PMN phagosomes We will determine how SA upregulation of msrA1/B, YjiE, and other transcriptional responses to HOCl and other ..
  34. Mechanisms of Mycobacterial Antigen Processing
    Chinnaswamy Jagannath; Fiscal Year: 2013
    ..Due to phagosome maturation arrest, structural antigens of BCG vaccine remain sequestered within phagosomes while its secreted antigens are minimally processed for MHC-II and MHC-I peptide epitopes...
  35. Development of Chemical Tools to Manipulate Copper at the Host/Pathogen Interface
    Katherine J Franz; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Macrophage cells of the immune system concentrate copper (Cu) into phagosomes to intensify microbial killing, while microbes counteract by upregulating Cu resistance pathways...
  36. Host-Pathogen interactions between Salmonella and Toll-like receptors
    Gregory M Barton; Fiscal Year: 2013
    ..This requirement is based on TLR-dependent acidification of Salmonella containing phagosomes. In this application, we build on these findings and explore the consequences of TLR activation for Salmonella ..
  37. Nanoparticle delivery system for antiturberculosis drug
    Leonid Heifets; Fiscal Year: 2004
    ..We will test the hypothesis that increasing the intracellular accumulation, particularly in phagosomes harboring M...
  38. Copper transport in Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2013
    ..Copper inside Mtb-containing phagosomes is increased upon stimulation of macrophages with interferon-[unreadable] to concentrations that are sufficient ..
  39. Type IV Secretion Systems in Legionella pneumophila
    Howard M Steinman; Fiscal Year: 2010
    ..is a fresh water bacterium that can invade amoebae and macrophages and replicate in membrane bound phagosomes. These virulence phenotypes require a Type IV secretion system (T4SS), currently proposed to be the T4SS encoded ..
  40. Phagocytic Removal of Apoptotic Cells
    Zheng Zhou; Fiscal Year: 2013
    ..b>Phagosomes undergo a "maturation" process through a series of membrane trafficking events and the end result is the ..
  41. Immunobiology of CTL Responses to Exogenous Antigen
    Kenneth L Rock; Fiscal Year: 2013
    ..Our second Aim will address the major unresolved issue of how antigens in the P2C pathway are dislocated from phagosomes into the cytosol. We hypothesize that two distinct mechanisms may be involved...
  42. RPE Cell Biology of Myosin VIIa
    David S Williams; Fiscal Year: 2013
    ..developments by: (1) Studying the roles of myosin VIIa and microtubule motors in the transport of melanosomes, phagosomes, and lysosomes in the RPE;(2) Identifying and testing the function of microtubule-binding domain(s) of myosin ..
  43. LISTERIA MONOCYTOGENES AND PHAGOSOME MEMBRANE TRAFFIC
    Philip Stahl; Fiscal Year: 2003
    ..LM is internalized into phagosomes where it actively inhibits maturation of the phagosome. Virulent LM escapes to the cytoplasm...
  44. Antibody mediated intracellular bacterial elimination
    Gary Winslow; Fiscal Year: 2006
    ..Aim #4 will utilize an in vitro infection model to determine if antibodies promote the fusion of phagosomes with lysosomes, and if antibodies can access bacteria in early endosomal compartments...
  45. CONTROL OF PHAGOCYTE NADPH OXIDASE BY CYTOSOLIC PROTEINS
    Gary Bokoch; Fiscal Year: 2005
    ..that encode the complex of cytosolic oxidase proteins to associate with the flavocytochrome to form a isolated phagosomes and cellular fractions, will be used to study the formation of the active enzyme and determine the roles of ..
  46. CD4+ T CELLS RESPONDING TO SALMONELLA INFECTION
    Brad Cookson; Fiscal Year: 2005
    ..Abstract): Salmonella, Mycobacterium and Histoplasma are facultative intracellular pathogens that live inside phagosomes of host macrophages...
  47. The function of CARD9 in innate immune responses
    Xin Lin; Fiscal Year: 2012
    ..Interestingly, we have found that CARD9 physically associates with bacteria-containing phagosomes and is directly involved in the clearance of infected bacteria in macrophages...
  48. SPATIOTEMPORAL ANALYSES OF NEONATAL HOST RESPONSE
    Christopher Contag; Fiscal Year: 2002
    ..The Nramp1 protein localizes to phagosomes and the plasma membrane, appears to be involved in a pathway leading to macrophage activation and antigen ..
  49. DISULFIDE SURFACE STRUCTURE IN LEGIONELLA PATHOGENESIS
    Paul Hoffman; Fiscal Year: 1990
    ..The legionellae are invasive and reside in the phagosomes of both professional and non-professional phagocytes where they abrogate phagolysosomal fusion by unknown ..
  50. EVASION OF MACROPHAGE LYSOSOMES BY L PNEUMOPHILA
    MICHELE SWANSON; Fiscal Year: 2001
    ..The goal of this work is to understand how L.pneumophila phagosomes avoid fusion with lysosomes. L...
  51. Mycobacterium tuberculosis in vivo gene expression
    James Graham; Fiscal Year: 2005
    ..tuberculosis genes which are expressed in response to growth within cultured human macrophage phagosomes. The first aim of the proposed work is to make bacterial strains specifically inhibited in expression of these ..
  52. Iron Transport Mechanisms of Francisella tularensis
    Girija Ramakrishnan; Fiscal Year: 2004
    ..In the mammalian host, the bacterium is an intracellular pathogen, replicating within membrane-bound phagosomes in macrophages and other cells...
  53. Protein Kinase C in Macrophage Activation
    MICHELLE LENNARTZ; Fiscal Year: 2006
    ..We will test the hypothesis that PKC localization to phagosomes is mediated by one or more regions within the regulatory domain...
  54. HUMAN CELLULAR IMMUNITY TO TOXOPLASMA GONDII
    Tyler Curiel; Fiscal Year: 1999
    ..Intracellular tachyzoites form a structure known as the parasitophorous vacuole (PV), which does not fuse with phagosomes, does not acidify normally, and which acts as a molecular sieve, controlling the entry and egress of various ..
  55. Mycobacterium tuberculosis phagosome maturation
    Lakshmi Ramachandra; Fiscal Year: 2009
    ..PARAGRAPH] Phagosome maturation is mediated by the recruitment or elimination of select host proteins from phagosomes. Therefore, host proteins that mediate this process are differentially expressed on mature versus immature ..
  56. Characterization of the M. tuberculosis phagosome
    MARCUS HORWITZ; Fiscal Year: 2007
    ..We have established that, whereas phagosomes containing dead M. tuberculosis proceed to phagolysosomes, live M...
  57. MECHANISMS OF ACUTE VASCULAR INJURY
    MARLYS GEE; Fiscal Year: 1993
    ..regulated neutrophils would accumulate in the alveolar airspaces in response to bacterial seeding and would form phagosomes, but killing would be impaired...
  58. MANIPULATION OF PHAGOCYTOSIS IN MACROPHAGES
    Joel Swanson; Fiscal Year: 1999
    ..Listeria monocytogenes is an intracellular pathogen that survives by passing from phagosomes into cytoplasm...
  59. Role of IFN-gamma Induced Macrophage GTPases in Brucella Killing
    James Cardelli; Fiscal Year: 2007
    ..7 macrophages, and that IFN-gamma treatment resulted in increased fusion of these phagosomes with lysosomes, thus preventing bacterial replication...
  60. REGULATION OF PHAGOCYTOSIS
    James Cardelli; Fiscal Year: 2001
    ..b>Phagosomes containing pathogenic organisms are usually modified by a series of fusion and fission reactions, involving ..
  61. Gender and Host Defense Against Pneumonia
    Lester Kobzik; Fiscal Year: 2009
    ..Specific Aims: Aim 1 will test the postulate that NOS3-production of NO acts within phagosomes where it interacts with NADPH oxidase-derived superoxide to form the potent microbicide, peroxynitrite...
  62. SURFACTANTS EFFECT ON TUBERCULOSIS IN THE LUNG
    Robert Hunter; Fiscal Year: 1999
    ..Electron microscopy will be used to assess lysosome-phagosomes fusion and related phenomena...
  63. NRAMPL AND PHAGOCYTE FUNCTION
    Philippe Gros; Fiscal Year: 2000
    ..Nramp1 is expressed in the lysosomal compartment of macrophages and is targeted to the membrane of bacterial phagosomes soon after phagocytosis...