endosomes

Summary

Summary: Cytoplasmic vesicles formed when COATED VESICLES shed their CLATHRIN coat. Endosomes internalize macromolecules bound by receptors on the cell surface.

Top Publications

  1. pmc Endosome maturation
    Jatta Huotari
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 30:3481-500. 2011
  2. ncbi Ceramide triggers budding of exosome vesicles into multivesicular endosomes
    Katarina Trajkovic
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany
    Science 319:1244-7. 2008
  3. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
  4. ncbi The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
  5. ncbi Endocytic recycling
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 5:121-32. 2004
  6. ncbi The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Cell 151:1256-69. 2012
  7. ncbi Rab conversion as a mechanism of progression from early to late endosomes
    Jochen Rink
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 122:735-49. 2005
  8. pmc Conformational biosensors reveal GPCR signalling from endosomes
    Roshanak Irannejad
    Department of Psychiatry, University of California, San Francisco, California 94158, USA
    Nature 495:534-8. 2013
  9. pmc SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors
    Paul Temkin
    Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158, USA
    Nat Cell Biol 13:715-21. 2011
  10. pmc Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array
    Martin Schuster
    Department of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom
    Mol Biol Cell 22:3645-57. 2011

Research Grants

Detail Information

Publications396 found, 100 shown here

  1. pmc Endosome maturation
    Jatta Huotari
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 30:3481-500. 2011
    ..In this review, we focus on endosomes in the classical endocytic pathway, and on the programme of changes that lead to the formation and maturation of ..
  2. ncbi Ceramide triggers budding of exosome vesicles into multivesicular endosomes
    Katarina Trajkovic
    Centre for Biochemistry and Molecular Cell Biology, University of Gottingen, 37073 Gottingen, Germany
    Science 319:1244-7. 2008
    Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu...
  3. ncbi Signals for sorting of transmembrane proteins to endosomes and lysosomes
    Juan S Bonifacino
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Biochem 72:395-447. 2003
    Sorting of transmembrane proteins to endosomes and lysosomes is mediated by signals present within the cytosolic domains of the proteins. Most signals consist of short, linear sequences of amino acid residues...
  4. ncbi The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
    ..The involvement of the ESCRT machinery in suppressing diseases such as cancer, neurodegeneration and infections underscores its importance to the cell...
  5. ncbi Endocytic recycling
    Frederick R Maxfield
    Department of Biochemistry, Weill Medical College of Cornell University, 1300 York Avenue, New York, New York 10021, USA
    Nat Rev Mol Cell Biol 5:121-32. 2004
    ..most membrane proteins and lipids return to the cell surface, but some membrane components are delivered to late endosomes or the Golgi...
  6. ncbi The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes
    Eisuke Itakura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Cell 151:1256-69. 2012
    ..These findings reveal a mechanism by which the SNARE protein is available to the completed autophagosome...
  7. ncbi Rab conversion as a mechanism of progression from early to late endosomes
    Jochen Rink
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 122:735-49. 2005
    ..The small GTPases Rab 5 and Rab 7 are key determinants of early and late endosomes, organizing effector proteins into specific membrane subdomains...
  8. pmc Conformational biosensors reveal GPCR signalling from endosomes
    Roshanak Irannejad
    Department of Psychiatry, University of California, San Francisco, California 94158, USA
    Nature 495:534-8. 2013
    ..These findings provide direct support for the hypothesis that canonical GPCR signalling occurs from endosomes as well as the plasma membrane, and suggest a versatile strategy for probing dynamic conformational change in ..
  9. pmc SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors
    Paul Temkin
    Department of Psychiatry, University of California at San Francisco, San Francisco, California 94158, USA
    Nat Cell Biol 13:715-21. 2011
    ..The present results identify a role for retromer in endocytic trafficking of signalling receptors, in regulating a receptor-linked signalling pathway, and in mediating direct endosome-to-plasma membrane traffic...
  10. pmc Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array
    Martin Schuster
    Department of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom
    Mol Biol Cell 22:3645-57. 2011
    ..In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde)...
  11. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
    ..CatB and CatB/CatL inhibitors diminish the multiplication of infectious EboV-Zaire in cultured cells and may merit investigation as anti-EboV drugs...
  12. ncbi Systems survey of endocytosis by multiparametric image analysis
    Claudio Collinet
    Max Planck Institute for Molecular Cell Biology and Genetics, Germany
    Nature 464:243-9. 2010
    ..regulate the endocytic system, and identified new genes involved in cargo sorting to a subset of signalling endosomes. A systems analysis by Bayesian networks further showed that the number, size, concentration of cargo and ..
  13. pmc Microautophagy of cytosolic proteins by late endosomes
    Ranjit Sahu
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Cell 20:131-9. 2011
    ..The delivery of autophagic cargo to late endosomes for complete or partial degradation has also been described...
  14. ncbi Identification of the switch in early-to-late endosome transition
    Dmitry Poteryaev
    Biozentrum, University of Basel, Klingelbergstrasse 70, Basel 4056, Switzerland
    Cell 141:497-508. 2010
    Sequential transport from early to late endosomes requires the coordinated activities of the small GTPases Rab5 and Rab7...
  15. pmc TBC1D14 regulates autophagosome formation via Rab11- and ULK1-positive recycling endosomes
    Andrea Longatti
    Cancer Research UK London Research Institute, WC2A 3PF London, England, UK
    J Cell Biol 197:659-75. 2012
    ..Overexpressed TBC1D14 tubulates ULK1-positive recycling endosomes (REs), impairing their function and inhibiting autophagosome formation...
  16. pmc Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer
    Matthew N J Seaman
    Cambridge Institute for Medical Research, University of Cambridge, Wellcome Trust MRC Building, Addenbrookes Hospital, Hills Road, Cambridge CB2 2XY, England, UK
    J Cell Biol 165:111-22. 2004
    ..We have localized mVPS26 to multivesicular body endosomes by electron microscopy, and through the use of CD8 reporter protein constructs have examined the effect of loss ..
  17. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    ..We describe a new member of the low-density lipoprotein receptor superfamily, RME-2, identified in our screens for endocytosis defective mutants. We show that RME-2 is the C. elegans yolk receptor...
  18. pmc Hepatitis C virus entry depends on clathrin-mediated endocytosis
    Emmanuelle Blanchard
    Equipe Hepatite C, CNRS UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:6964-72. 2006
    ..These data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment...
  19. pmc A dynein loading zone for retrograde endosome motility at microtubule plus-ends
    J H Lenz
    Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
    EMBO J 25:2275-86. 2006
    In the fungus Ustilago maydis, early endosomes move bidirectionally along microtubules (MTs) and facilitate growth by local membrane recycling at the tip of the infectious hypha...
  20. ncbi Regulated transport of the glucose transporter GLUT4
    Nia J Bryant
    Garvan Institute of Medical Research, 384 Victoria Road, Darlinghurst, New South Wales 2010, Australia
    Nat Rev Mol Cell Biol 3:267-77. 2002
    ....
  21. pmc Role of endosomal cathepsins in entry mediated by the Ebola virus glycoprotein
    Kathryn Schornberg
    Department of Microbiology, University of Virginia, 1300 Jefferson Park Ave, Charlottesville, Virginia 22908 0734, USA
    J Virol 80:4174-8. 2006
    ..These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion...
  22. ncbi The V-type H+-ATPase in vesicular trafficking: targeting, regulation and function
    Vladimir Marshansky
    Program in Membrane Biology, Center for Systems Biology, Simches Research Center, CPZN No 8212, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    Curr Opin Cell Biol 20:415-26. 2008
    ..Here, we will review these studies with emphasis on novel direct roles of V-ATPase in the regulation of vesicular trafficking events...
  23. pmc Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
    Mark T Howes
    The Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 190:675-91. 2010
    ..These studies provide the first quantitative ultrastructural analysis and molecular characterization of the major endocytic pathway in fibroblasts, a pathway that provides rapid membrane turnover at the leading edge of migrating cells...
  24. ncbi Systematic analysis of SNARE molecules in Arabidopsis: dissection of the post-Golgi network in plant cells
    Tomohiro Uemura
    Graduate School of Biostudies, Kyoto University, Yoshida nihonmatsu, Sakyo ku, Kyoto 606 8501, Japan
    Cell Struct Funct 29:49-65. 2004
    ..compartments: 6 in the endoplasmic reticulum, 9 in the Golgi apparatus, 4 in the trans-Golgi network (TGN), 2 in endosomes, 17 on the plasma membrane, 7 in both the prevacuolar compartment (PVC) and vacuoles, 2 in TGN/PVC/vacuoles, and ..
  25. ncbi Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover
    Gianni M Di Guglielmo
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Cell Biol 5:410-21. 2003
    ..Thus, segregation of TGF-beta receptors into distinct endocytic compartments regulates Smad activation and receptor turnover...
  26. ncbi Feature point tracking and trajectory analysis for video imaging in cell biology
    I F Sbalzarini
    Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
    J Struct Biol 151:182-95. 2005
    ..Its applicability is demonstrated in three case studies involving transport of low-density lipoproteins in endosomes, motion of fluorescently labeled Adenovirus-2 particles along microtubules, and tracking of quantum dots on the ..
  27. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ..Fusion between early endosomes requires phosphatidylinositol-3-OH kinase (PI(3)K) activity as well as the small GTPase Rab5...
  28. pmc The retromer complex - endosomal protein recycling and beyond
    Matthew N J Seaman
    University of Cambridge, Cambridge Institute for Medical Research, UK
    J Cell Sci 125:4693-702. 2012
    ..In this Commentary, I will highlight recent advances in the understanding of retromer-mediated endosomal protein sorting and discuss how retromer contributes to a diverse set of physiological processes...
  29. ncbi APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment
    Marta Miaczynska
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 116:445-56. 2004
    ..This pathway operates via APPL1 and APPL2, two Rab5 effectors, which reside on a subpopulation of endosomes. In response to extracellular stimuli such as EGF and oxidative stress, APPL1 translocates from the membranes to ..
  30. pmc Neuronal sorting protein-related receptor sorLA/LR11 regulates processing of the amyloid precursor protein
    Olav M Andersen
    Max Delbrueck Center for Molecular Medicine, D 13125 Berlin, Germany
    Proc Natl Acad Sci U S A 102:13461-6. 2005
    ..Its homology to sorting receptors that shuttle between the plasma membrane, endosomes, and the Golgi suggests a related function in neuronal trafficking processes...
  31. ncbi Endocytosis by random initiation and stabilization of clathrin-coated pits
    Marcelo Ehrlich
    Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 118:591-605. 2004
    Clathrin-coated vesicles carry traffic from the plasma membrane to endosomes. We report here the real-time visualization of cargo sorting and endocytosis by clathrin-coated pits in living cells...
  32. ncbi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
    ..of phosphatidylserine and found that it binds the cytosolic leaflets of the plasma membrane, as well as endosomes and lysosomes...
  33. ncbi Rab27a and Rab27b control different steps of the exosome secretion pathway
    Matias Ostrowski
    INSERM U932, 26 Rue d Ulm, 75005 Paris, France
    Nat Cell Biol 12:19-30; sup pp 1-13. 2010
    ..vesicles that share structural and biochemical characteristics with intraluminal vesicles of multivesicular endosomes (MVEs)...
  34. ncbi Endocytosis and signaling
    Harald W Platta
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, 0310 Oslo, Norway
    Curr Opin Cell Biol 23:393-403. 2011
    ..These are commonly divided into early endosomes, recycling endosomes, and late endosomes...
  35. pmc Coordination of Rab8 and Rab11 in primary ciliogenesis
    Andreas Knödler
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 107:6346-51. 2010
    ..downstream effector of Rab11, which functions in membrane trafficking from the trans-Golgi network and recycling endosomes. Rab11, in its GTP-bound form, interacts with Rabin8 and kinetically stimulates the guanine nucleotide-exchange ..
  36. pmc The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    M Babst
    Division of Cellular Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine La Jolla, CA 92093 0668, USA
    EMBO J 17:2982-93. 1998
    ..Together, the data suggest that the Vps4 ATPase catalyzes the release (uncoating) of an endosomal membrane-associated class E protein complex(es) required for normal morphology and sorting activity of the endosome...
  37. ncbi Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins
    Valerie Uytterhoeven
    VIB, Department of Molecular and Developmental Genetics, Program in Molecular and Developmental Genetics, Program in Cognitive and Molecular Neuroscience, Laboratory of Neuronal Communication, Herestraat 49, 3000 Leuven, Belgium
    Cell 145:117-32. 2011
    Exchange of proteins at sorting endosomes is not only critical to numerous signaling pathways but also to receptor-mediated signaling and to pathogen entry into cells; however, how this process is regulated in synaptic vesicle cycling ..
  38. ncbi Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding
    Robert D Fisher
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Cell 128:841-52. 2007
    ..ALIX therefore serves as a flexible, extended scaffold that connects retroviral Gag proteins to ESCRT-III and other cellular-budding machinery...
  39. pmc A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast
    M N Seaman
    Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093 0668, USA
    J Cell Biol 142:665-81. 1998
    ..Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval...
  40. pmc The fifth adaptor protein complex
    Jennifer Hirst
    University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK
    PLoS Biol 9:e1001170. 2011
    ..Thus, AP-5 is an evolutionarily ancient complex, which is involved in endosomal sorting, and which has links with hereditary spastic paraplegia...
  41. ncbi Multisubunit tethering complexes and their role in membrane fusion
    Cornelia Bröcker
    University of Osnabruck, Department of Biology Chemistry, Barbarastrasse 13, 49076 Osnabruck, Germany
    Curr Biol 20:R943-52. 2010
    ....
  42. ncbi The protein network of HIV budding
    Uta K von Schwedler
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Cell 114:701-13. 2003
    ..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses...
  43. ncbi Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
    Sandra S Diebold
    Immunobiology Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, UK
    Science 303:1529-31. 2004
    ..These results identify ssRNA as a ligand for TLR7 and suggest that cells of the innate immune system sense endosomal ssRNA to detect infection by RNA viruses...
  44. ncbi The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex
    Emmanuel Derivery
    Institut Curie, Centre de Recherche, 75248 Paris Cedex 05, France
    Dev Cell 17:712-23. 2009
    ..Here, we show that WASH generates an actin network on a restricted domain of sorting and recycling endosomes. We found that WASH belongs to a multiprotein complex containing seven subunits, including the heterodimer of ..
  45. pmc Sorting of the Alzheimer's disease amyloid precursor protein mediated by the AP-4 complex
    Patricia V Burgos
    Cell Biology and Metabolism Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 18:425-36. 2010
    ..Disruption of the APP-AP-4 interaction decreases localization of APP to endosomes and enhances gamma-secretase-catalyzed cleavage of APP to the pathogenic amyloid-beta peptide...
  46. pmc Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling
    Eugen Brailoiu
    Department of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA
    J Cell Biol 186:201-9. 2009
    ..Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP...
  47. pmc Microtubule-based transport in filamentous fungi
    Martin J Egan
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, United States
    Curr Opin Microbiol 15:637-45. 2012
    ..microtubules in nuclear positioning is well studied, but recent work has expanded the range of cargos to include endosomes, messenger RNA, secretory vesicles, peroxisomes, and nuclear pore complexes, reflecting the diversity of ..
  48. pmc Retromer
    Juan S Bonifacino
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, Building 18T Room 101, National Institutes of Health, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 20:427-36. 2008
    The retromer is a heteropentameric complex that associates with the cytosolic face of endosomes and mediates retrograde transport of transmembrane cargo from endosomes to the trans-Golgi network...
  49. ncbi Plasmacytoid dendritic cells sense self-DNA coupled with antimicrobial peptide
    Roberto Lande
    Department of Immunology, M D Anderson Cancer Center, University of Texas, Houston, Texas 77030, USA
    Nature 449:564-9. 2007
    ..Thus, our data uncover a fundamental role of an endogenous antimicrobial peptide in breaking innate tolerance to self-DNA and suggest that this pathway may drive autoimmunity in psoriasis...
  50. ncbi Long-distance movement of Aspergillus nidulans early endosomes on microtubule tracks
    Juan F Abenza
    Departamento de Microbiologia Molecular, Centro de Investigaciones Biologicas CSIC, Ramiro de Maeztu 9, Madrid, Spain
    Traffic 10:57-75. 2009
    ..Aspergillus nidulans RabA(Rab5) localizes to a population of endosomes that show long range bidirectional movement on microtubule (MT) tracks and are labelled with FM4-64 shortly ..
  51. pmc Transient binding of dynein controls bidirectional long-range motility of early endosomes
    Martin Schuster
    Department of Biosciences, University of Exeter, Exeter EX4 4PE, United Kingdom
    Proc Natl Acad Sci U S A 108:3618-23. 2011
    ..Live cell imaging shows that binding of retrograde moving dynein to anterograde moving endosomes changes the transport direction of the organelles...
  52. ncbi Autophagy and multivesicular bodies: two closely related partners
    C M Fader
    Laboratorio de Biologia Celular y Molecular, Instituto de Histologia y Embriologia IHEM CONICET, Facultad de Ciencias Medicas, Universidad Nacional de Cuyo, Casilla de Correo 56, Centro Universitario, Parque General San Martín, 5500 Mendoza, Argentina
    Cell Death Differ 16:70-8. 2009
    In the majority of cell types, multivesicular bodies (MVBs) are a special kind of late endosomes, crucial intermediates in the internalization of nutrients, ligands and receptors through the endolysosomal system...
  53. pmc The novel lipid raft adaptor p18 controls endosome dynamics by anchoring the MEK-ERK pathway to late endosomes
    Shigeyki Nada
    Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    EMBO J 28:477-89. 2009
    ..adaptor protein, p18, is involved in controlling endosome dynamics by anchoring the MEK1-ERK pathway to late endosomes. p18 is anchored to lipid rafts of late endosomes through its N-terminal unique region...
  54. pmc Tug-of-war between dissimilar teams of microtubule motors regulates transport and fission of endosomes
    Virupakshi Soppina
    Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400005, India
    Proc Natl Acad Sci U S A 106:19381-6. 2009
    ..Our work may provide a platform to understand intracellular transport of a variety of organelles in terms of measurable quantities...
  55. ncbi The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network
    Tatyana Y Belenkaya
    Division of Developmental Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA
    Dev Cell 14:120-31. 2008
    ..Importantly, Vps35 colocalizes in endosomes and interacts with Wls. Wls becomes unstable in the absence of retromer activity...
  56. ncbi Exosomes: endosomal-derived vesicles shipping extracellular messages
    Benoit Fevrier
    Institut Curie, Research Section, CNRS UMR 144, 26 Rue d Ulm, 75005 Paris, France
    Curr Opin Cell Biol 16:415-21. 2004
    Exosomes are membrane vesicles released into the extracellular environment upon exocytic fusion of multivesicular endosomes with the cell surface...
  57. pmc Sorting nexins provide diversity for retromer-dependent trafficking events
    Peter J Cullen
    Henry Wellcome Integrated Signalling Laboratories, School of Biochemistry, Medical Sciences Building, University Walk, University of Bristol, Bristol, BS8 1TD, UK
    Nat Cell Biol 14:29-37. 2012
    ..Here we discuss how retromer complexes regulate endosomal sorting, and describe how this is generating exciting new insight into the central role played by endosomal sorting in development and homeostasis of normal tissues...
  58. pmc The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics
    Michael E Harbour
    Department of Clinical Biochemistry, University of Cambridge, Cambridge Institute for Medical Research, Wellcome Trust MRC Building, Addenbrookes Hospital, Cambridge CB2 0XY, UK
    J Cell Sci 123:3703-17. 2010
    ....
  59. ncbi Ubiquitination of alpha 5 beta 1 integrin controls fibroblast migration through lysosomal degradation of fibronectin-integrin complexes
    Viola Hélène Lobert
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway
    Dev Cell 19:148-59. 2010
    ..a fraction of the endocytosed fibronectin receptor, alpha 5 beta 1 integrin, is sorted into multivesicular endosomes together with fibronectin and degraded in lysosomes...
  60. pmc The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein
    Nanette Van Damme
    The San Diego Department of Veterans Affairs Healthcare System, San Diego, CA 92161, USA
    Cell Host Microbe 3:245-52. 2008
    ..Endogenous BST-2 colocalizes with the HIV-1 structural protein Gag in endosomes and at the plasma membrane, suggesting that BST-2 traps virions within and on infected cells...
  61. pmc Recognition of single-stranded RNA viruses by Toll-like receptor 7
    Jennifer M Lund
    Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 101:5598-603. 2004
    ..These results demonstrate microbial ligand recognition by TLR7 and provide insights into the pathways used by the innate immune cells in the recognition of viral pathogens...
  62. ncbi Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes
    J M Escola
    Department of Cell Biology, Utrecht University School of Medicine, AZU, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    J Biol Chem 273:20121-7. 1998
    ....
  63. ncbi Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes
    Robert Köchl
    Cancer Research UK, London Research Institute, Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Traffic 7:129-45. 2006
    ..which sequester cytosolic components and organelles for degradation after fusion with protease-containing endosomes. To determine the role of microtubules in autophagy, we used nocodazole and vinblastine to disrupt microtubules ..
  64. ncbi Nanotubular highways for intercellular organelle transport
    Amin Rustom
    Interdisciplinary Center of Neuroscience IZN, Institute of Neurobiology, University of Heidelberg, INF 364, Heidelberg 69120, Germany
    Science 303:1007-10. 2004
    ..Accordingly, we propose a novel biological principle of cell-to-cell interaction based on membrane continuity and intercellular transfer of organelles...
  65. pmc Apical trafficking in epithelial cells: signals, clusters and motors
    Ora A Weisz
    Department of Medicine and Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    J Cell Sci 122:4253-66. 2009
    ..They identified the trans-Golgi network and recycling endosomes as the compartments that carried out apical-basolateral sorting...
  66. pmc Hrs, a FYVE finger protein localized to early endosomes, is implicated in vesicular traffic and required for ventral folding morphogenesis
    M Komada
    Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 13:1475-85. 1999
    ..Abnormally enlarged early endosomes were detected in the mutants in several tissues including definitive endoderm, suggesting that a deficiency in ..
  67. ncbi Kinesin-3 and dynein mediate microtubule-dependent co-transport of mRNPs and endosomes
    Sebastian Baumann
    Heinrich Heine University Dusseldorf, Institute for Microbiology, 40204 Düsseldorf, Germany
    J Cell Sci 125:2740-52. 2012
    ..we additionally demonstrate that Rrm4-containing mRNPs colocalise with the t-SNARE Yup1 on shuttling endosomes and that functional endosomes are essential for mRNP movement...
  68. pmc Snapin recruits dynein to BDNF-TrkB signaling endosomes for retrograde axonal transport and is essential for dendrite growth of cortical neurons
    Bing Zhou
    Synaptic Functions Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 35, Room 2B 215, 35 Convent Drive, Bethesda, MD 20892 3706, USA
    Cell Rep 2:42-51. 2012
    Neurotrophin signaling is crucial for neuron growth. While the "signaling endosomes" hypothesis is one of the accepted models, the molecular machinery that drives retrograde axonal transport of TrkB signaling endosomes is largely unknown...
  69. pmc The p97 ATPase associates with EEA1 to regulate the size of early endosomes
    Harish N Ramanathan
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Res 22:346-59. 2012
    ..Inhibition of p97 either by siRNA or a pharmacological inhibitor results in clustering and enlargement of early endosomes, which is associated with an altered trafficking pattern for an endocytic cargo...
  70. pmc Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
    Cecilia N Arighi
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 165:123-33. 2004
    ..mannose 6-phosphate receptor (CI-MPR) mediates sorting of lysosomal hydrolase precursors from the TGN to endosomes. After releasing the hydrolase precursors into the endosomal lumen, the unoccupied receptor returns to the TGN ..
  71. pmc Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150 Glued and late endosome positioning
    Nuno Rocha
    Division of Cell Biology, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands
    J Cell Biol 185:1209-25. 2009
    Late endosomes (LEs) have characteristic intracellular distributions determined by their interactions with various motor proteins...
  72. ncbi Endosomal sorting and signalling: an emerging role for sorting nexins
    Peter J Cullen
    Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK
    Nat Rev Mol Cell Biol 9:574-82. 2008
    ..Here we discuss the sorting nexins family of proteins and propose that they have a fundamental role in orchestrating the formation of protein complexes that are involved in endosomal sorting and signalling...
  73. ncbi Not just a sink: endosomes in control of signal transduction
    Marta Miaczynska
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Curr Opin Cell Biol 16:400-6. 2004
    ..By deciphering the interplay between endocytosis and signalling, we will be able to gain a more sophisticated level of understanding of signal transduction mechanisms...
  74. pmc Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA
    Mol Biol Cell 23:3215-28. 2012
    ..Of interest, although EEA1(+) endosomes were enlarged, collapsed, and devoid of filamentous-actin and Arp2/3 in WASHout MEFs, we did not observe ..
  75. pmc Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease
    Maria Filimonenko
    Centre for Cancer Biomedicine, University of Oslo and Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Biol 179:485-500. 2007
    ..Together, our data indicate that efficient autophagic degradation requires functional MVBs and provide a possible explanation to the observed neurodegenerative phenotype seen in patients with CHMP2B mutations...
  76. pmc MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a
    Juliati Rahajeng
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198 5870, USA
    Traffic 13:82-93. 2012
    ..Over-expression of active Rab35 impairs the recruitment of MICAL-L1 to tubular recycling endosomes, whereas Rab35 depletion promotes enhanced MICAL-L1 localization to these structures...
  77. ncbi Uncoating of human rhinoviruses
    Renate Fuchs
    Department of Pathophysiology, Medical University of Vienna, Vienna, Austria
    Rev Med Virol 20:281-97. 2010
    ..Triggered by ICAM-1 and/or the low pH environment in endosomes the virions undergo conformational alterations giving rise to hydrophobic subviral particles...
  78. pmc A clathrin-dependent pathway leads to KRas signaling on late endosomes en route to lysosomes
    Albert Lu
    Departament de Biologia Cellular, Immunologia i Neurociencies, Institut d Investigacions Biomediques August Pi i Sunyer, Serveis Cientificotecnics, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
    J Cell Biol 184:863-79. 2009
    ..From early endosomes, KRas but not Harvey Ras or neuroblastoma Ras is sorted and transported to late endosomes (LEs) and lysosomes...
  79. ncbi Expression of δ-toxin by Staphylococcus aureus mediates escape from phago-endosomes of human epithelial and endothelial cells in the presence of β-toxin
    Bernd Giese
    FunGene Competence Center for Functional Genomics, Ernst Moritz Arndt University, F L Jahnstrasse 15, Greifswald, Germany
    Cell Microbiol 13:316-29. 2011
    ..staphylococcal adhesins with extracellular proteins of mammalian cells and eventually resides in acidified phago-endosomes. Some staphylococcal strains have been shown to subsequently escape from this compartment...
  80. pmc Dengue virus ensures its fusion in late endosomes using compartment-specific lipids
    Elena Zaitseva
    Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America
    PLoS Pathog 6:e1001131. 2010
    ..the most prevalent mosquito-borne virus that infects up to 100 million people each year, fuses only in late endosomes, while activation of DEN protein fusogen glycoprotein E is triggered already at pH characteristic for early ..
  81. pmc Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport
    Giulia Pasqual
    Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland
    PLoS Pathog 7:e1002232. 2011
    ..Upon internalization, the viruses are delivered to acidified endosomes in a Rab5-independent manner bypassing classical routes of incoming vesicular trafficking...
  82. ncbi The dynamic Rab11-FIPs
    Conor P Horgan
    Molecular Cell Biology Laboratory, Department of Biochemistry, Biosciences Institute, University College Cork, Cork, Ireland
    Biochem Soc Trans 37:1032-6. 2009
    ..In this mini-review, we summarize the Rab11-FIP field and discuss, at molecular and cellular levels, the recent findings on FIP function...
  83. pmc Receptor trafficking controls weak signal delivery: a strategy used by c-Met for STAT3 nuclear accumulation
    Stephanie Kermorgant
    Department of Tumour Biology, Cancer Research UK Clinical Centre, Bart s and the London Queen Mary s School of Medicine and Dentistry, London EC1M 6BQ, England, UK
    J Cell Biol 182:855-63. 2008
    ..This response is triggered from peripheral endosomes. Thus, control of growth factor receptor traffic determines the nature of the signal output, providing novel ..
  84. pmc The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration
    Tobias Zech
    Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK
    J Cell Sci 124:3753-9. 2011
    ..Thus, we implicate WASH and Arp2/3-driven actin nucleation in receptor recycling leading to invasive motility...
  85. ncbi Heat shock protein 70 is secreted from tumor cells by a nonclassical pathway involving lysosomal endosomes
    Salamatu S Mambula
    Division of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 21 27 Burlington Avenue, Boston, MA 02215, USA
    J Immunol 177:7849-57. 2006
    ..Our experiments therefore suggest a mechanism for HSP70 secretion and binding to the surface of other cells that may be involved in recognition of the tumor cells by the immune system...
  86. ncbi Rab7: role of its protein interaction cascades in endo-lysosomal traffic
    Tuanlao Wang
    Institute for Biomedical Research, Xiamen University, Xiamen, Fujian, China 361005
    Cell Signal 23:516-21. 2011
    ..We summarize here the key protein interaction cascades of Rab7 by focusing on endo-lysosomal trafficking regulated by its interaction with HOPs, RILP, ORP1L, FYCO1 and Mon1/Sand1-CCZ1 complex...
  87. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
    ..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
  88. ncbi Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells
    Oleg O Glebov
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 8:46-54. 2006
    ..We applied a ferro-fluid-based purification of endosomes to identify endosomal proteins...
  89. pmc LMP1 association with CD63 in endosomes and secretion via exosomes limits constitutive NF-κB activation
    Frederik J Verweij
    Department of Pathology, Cancer Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands
    EMBO J 30:2115-29. 2011
    ..that endogenous LMP1 escapes degradation upon accumulation within intraluminal vesicles of multivesicular endosomes and secretion via exosomes...
  90. pmc FGD2, a CDC42-specific exchange factor expressed by antigen-presenting cells, localizes to early endosomes and active membrane ruffles
    Christoph Huber
    Department of Immunology and Microbial Sciences, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 283:34002-12. 2008
    ..FGD2 has a complex intracellular localization, with concentrations found in membrane ruffles and early endosomes. Although endosomal localization of FGD2 is dependent on a conserved FYVE domain, its C-terminal pleckstrin ..
  91. ncbi AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly
    Xinhong Dong
    Department of Pediatrics and Microbiology and Immunology, Vanderbilt University, Nashville, TN 37232, USA
    Cell 120:663-74. 2005
    ..These studies illuminate an early step in retroviral particle assembly and provide evidence that the trafficking of Gag to late endosomes is part of a productive particle assembly pathway.
  92. ncbi Defining macropinocytosis
    Markus C Kerr
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Traffic 10:364-71. 2009
    ..It is now well established that macropinocytosis significantly contributes to antigen presentation by the immune system and is exploited by a range of pathogens for cellular invasion and avoidance of immune surveillance...
  93. ncbi Localization of the MP1-MAPK scaffold complex to endosomes is mediated by p14 and required for signal transduction
    David Teis
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Dev Cell 3:803-14. 2002
    ..We show that p14 functions as an adaptor protein, which is required and sufficient to localize MP1 to endosomes. Reduction of MP1 or p14 protein levels by siRNAi results in defective signal transduction...
  94. ncbi PDGF-regulated rab4-dependent recycling of alphavbeta3 integrin from early endosomes is necessary for cell adhesion and spreading
    M Roberts
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7EH, United Kingdom
    Curr Biol 11:1392-402. 2001
    ..In serum-starved cells, internalized integrins were transported through rab4-positive, early endosomes and arrived at the rab11-positive, perinuclear recycling compartment approximately 30 min after endocytosis...
  95. pmc Cellular entry of ebola virus involves uptake by a macropinocytosis-like mechanism and subsequent trafficking through early and late endosomes
    Mohammad F Saeed
    Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America
    PLoS Pathog 6:e1001110. 2010
    ..following internalization, the virus enters the endolysosomal pathway and is trafficked through early and late endosomes, but the exact site of membrane fusion and nucleocapsid penetration in the cytoplasm remains unclear...
  96. ncbi HIV-1 buds and accumulates in "nonacidic" endosomes of macrophages
    Mabel Jouve
    Institut Curie, INSERM U653, 26 rue d Ulm 75248 Paris Cedex 05, France
    Cell Host Microbe 2:85-95. 2007
    ..This may prevent its fusion with lysosomes, since acidification is required for the maturation of endosomes. Thus, HIV-1 has developed a strategy for survival within infected macrophages involving prevention of ..
  97. pmc Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments
    Yuyang Tang
    Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, TN 37208, USA
    J Virol 83:7982-95. 2009
    ..Taken together, our findings demonstrate that intact intracellular cholesterol trafficking pathways mediated by NPC1 are needed for efficient HIV-1 production...
  98. ncbi Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery
    Jez G Carlton
    Department of Infectious Diseases, King s College London School of Medicine, London, UK
    Science 316:1908-12. 2007
    ..Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events...
  99. pmc Recruitment of actin modifiers to TrkA endosomes governs retrograde NGF signaling and survival
    Anthony W Harrington
    The Solomon H Snyder Department of Neuroscience and Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 146:421-34. 2011
    ..Although both promote axonal extension via the TrkA receptor, only NGF activates retrograde transport of TrkA endosomes to support neuronal survival...
  100. ncbi The role of ubiquitylation in receptor endocytosis and endosomal sorting
    Kaisa Haglund
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    J Cell Sci 125:265-75. 2012
    ..In addition, we discuss the importance of ubiquitin-dependent receptor endocytosis for the maintenance of cellular homeostasis and in the prevention of diseases such as cancer...
  101. pmc Endosomal phosphoinositides and human diseases
    Anne Sophie Nicot
    Department of Neurobiology and Genetics, Institut de Genetique et de Biologie Moleculaire et Cellulaire, INSERM U596, CNRS UMR 7104, Universite Louis Pasteur de Strasbourg, College de France, 67404 Illkirch, France
    Traffic 9:1240-9. 2008
    ..This review presents the roles of the endosomal PIs and their regulators and proposes defects of membrane remodeling as a common pathological mechanism for the corresponding diseases...

Research Grants67

  1. BONE MATRIX AND BONE RESORPTION
    Paul R Odgren; Fiscal Year: 2012
    ..Plekhm1 localizes late to endosomes in a manner dependent on GTP-bound Rab7...
  2. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..The pathway occupied by the transferrin receptor involves rapid cycling between the cell surface and early endosomes, delivers transferrin to every cell of vertebrate animals, and represents a remarkable natural delivery vehicle...
  3. NEURAL REGULATION OF PANCREATIC FUNCTION
    KIMBERLY SAUNDERS KIRKWOOD; Fiscal Year: 2012
    ..by examining the role of metalloendopeptidases that degrade proinflammatory peptides on the cell surface and in endosomes. We will determine whether neutral endopeptidase (NEP), a cell surface enzyme that degrades at least 11 peptides ..
  4. Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
    Robert L Nussbaum; Fiscal Year: 2013
    ..OCRL gene, which encodes Ocrl, a phosphatidylinositol(4,5)P2 5-phosphatase located in the trans-Golgi network, endosomes, plasma membrane ruffles, and clathrin-coated pits...
  5. The Mucolipin TRP Ion Channels
    Haoxing Xu; Fiscal Year: 2013
    ..family of Transient Receptor Potential (TRPML) proteins is predicted to encode ion channels of intracellular endosomes and lysosomes...
  6. Sphingosine 1-phosphate receptors in vascular homeostasis and pathology
    TIMOTHY TUN HLA; Fiscal Year: 2012
    ..We will critically test the hypothesis that selective subcellular targeting of S1P1R, namely, recycling between endosomes and plasma membrane or ubiquitin-based targeting to degradative organelle is an important aspect of regulation ..
  7. Regulation of Cardiac Kv Channel Expression
    Edwin S Levitan; Fiscal Year: 2013
    ..Our recent studies show that AII acts via endosomes and CamKII (calmodulin dependent protein kinase II) to induce biphasic activation of p38 kinase...
  8. ClC-3 Chloride Ion Channels in Vascular Smooth Muscle
    Fred Lamb; Fiscal Year: 2009
    ..superoxide (O2-") production in response to cytokines (TNF[unreadable] and IL-1[unreadable]) occurs in early endosomes following receptor endocytosis...
  9. STRUCTURE OF THE VACUOLAR ATPase
    Stephan Wilkens; Fiscal Year: 2013
    ..membranes of a wide variety of intracellular compartments like clathrin-coated vesicles, chromaffin granules, endosomes, lysosomes, synaptic vesicles, Golgi derived vesicles and the yeast vacuole...
  10. Control of liver autophagy by phosphatidic acid signaling
    GILBERT DIPAOLO; Fiscal Year: 2013
    ..acid is to promote the maturation of autophagosomes by facilitating the fusion of these organelles with endosomes/lysosomes...
  11. Regulation of the anti-phospholipid response in SLE
    Anne Davidson; Fiscal Year: 2013
    ..apoptotic particles by IgG anti-phospholipid antibodies results in delivery of nucleic acids to TLR containing endosomes. Engagement of TLRs induces both IFN1 that fosters plasma cell maturation and BAFF that enhances B cell survival ..
  12. Cellular Components Involved in Japanese Encephalitis Virus Entry
    Young Min Lee; Fiscal Year: 2013
    ..well known (particularly viral glycoprotein E, which attaches the virions to the cell surface, delivers them to endosomes, and catalyzes the viral-endosome membrane fusion), the host factors that participate in this multistep process ..
  13. SK channels: Roles and mechanisms for dendritic excitability and plasticity
    James G Maylie; Fiscal Year: 2012
    ..3) SK2 channels in the PSD undergo basal recycling through dynamin-dependent endocytosis and recycling endosomes. Upon the induction of LTP recycling is interrupted and the channels enter a population of late endosomes...
  14. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..the relative function of CHC22 and discovered that CHC22 participates uniquely in retrograde transport from endosomes to the TGN and thereby targets the GLUT4 glucose transporter to the insulin-responsive GLUT4 storage compartment ..
  15. The role of two-pore channels in integrative calcium signaling
    Michael X Zhu; Fiscal Year: 2013
    ..The proposed project will show that two-pore channels are NAADP receptors expressed on endosomes and lysosomes and they play important functions in regulating Ca2+ release from acidic organelles and shaping ..
  16. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2013
    ..Golgi outposts, and spine apparatuses for local protein synthesis;recycling and sorting endosomes for redistribution and degradation of membranes and proteins;and mitochondria for ATP production and calcium ..
  17. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, endosomes for membrane recycling, and smooth endoplasmic reticulum (SER) and mitochondria for calcium regulation...
  18. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Awni Hannun; Fiscal Year: 2013
    ..compartment (which has features of a previously-identified but not well-appreciated component of slow recycling endosomes), but also the co-translocation of many other plasma membrane/recycling receptors, channels, other proteins and ..
  19. V-ATPase H+ PUMP REGULATION IN FUEL ENERGY SELECTION
    Karlett J Parra; Fiscal Year: 2013
    ..Located at the membrane of lysosomes, vacuoles and endosomes, V-ATPases sustain the acidic luminal pH needed for protein sorting and degradation;and for entry of viruses and ..
  20. Arrestin Regulation of CRF1 Signaling: Mechanisms for Stress Resilience and PTSD
    Richard L Hauger; Fiscal Year: 2013
    ..mechanism, which inhibits G protein binding and internalizes the membrane receptor into cytosolic endosomes. We have demonstrated that a [unreadable]arrestin2 gene deletion in CRF-R1-expressing cells induces a 5-fold ..
  21. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..Delivery of these proteins to late endosomes and lysosomes is essential for proper acidification of these organelles In microglial cells...
  22. Cellular and molecular mechanisms of gp120 neurotoxicity
    Italo Mocchetti; Fiscal Year: 2012
    ..These experiments will be accompanied by histological analysis of gp120 immunoreactivity within endosomes or other cellular organelles, as well as by biochemical analyses of pro-inflammatory cytokines...
  23. Human Macrophage Sodium Channels: Novel Targets for Inflammatory Diseases
    Michael D Carrithers; Fiscal Year: 2013
    ..To characterize how the channel is activated, novel methods of single channel recordings in isolated endosomes will be combined with more traditional methods of whole cell patch clamp recordings in transfected cells that ..
  24. Receptor Tyrosine Kinase Sorting and Transactivation in Endosomes
    MARK LINDSAY GRIMES; Fiscal Year: 2010
    ..We hypothesize that receptor sorting into endosomes has functional significance for the intracellular location and duration of signaling and that signaling from ..
  25. Regulation of AKAP79 Postsynaptic Targeting and Signaling
    MARK L DELL'ACQUA; Fiscal Year: 2013
    ..N-terminal targeting domain in mediating its localization to postsynaptic lipid rafts and dendritic recycling endosomes. Importantly, we found that a palmitoylation deficient AKAP mutant altered regulation of spine enlargement, ..
  26. Regulation of the anti-phospholipid response in SLE
    Anne Davidson; Fiscal Year: 2009
    ..apoptotic particles by IgG anti-phospholipid antibodies results in delivery of nucleic acids to TLR containing endosomes. Engagement of TLRs induces both IFNαthat fosters plasma cell maturation and BAFF that decreases the ..
  27. Modulation of Rab7-Dependent Degradative Pathways by Novel Adenovirus Protein
    Cathleen R Carlin; Fiscal Year: 2012
    ..interacts with RILP and ORP1L, two known effectors for the Rab7 GTPase that governs transport from early to late endosomes and then to lysosomes...
  28. Activation of rapamycin-sensitive TORC1 by endomembrane amino acid transporters
    MARIA E CARDENAS-CORONA; Fiscal Year: 2013
    ..Intriguingly, TORC1 and the EGOC colocalize to endomembranes including those of endosomes and vacuoles, along with vacuolar amino acid transporters...
  29. EGF Receptor Signaling in Time and Space in Tumor Cells
    Alexander D Sorkin; Fiscal Year: 2012
    ..The activated EGFR is rapidly internalized into endosomes and sorted to lysosomes for degradation...
  30. How does misregulation of PI3,5P2 signaling lead to neurodegeneration?
    Lois S Weisman; Fiscal Year: 2013
    ..is present in all eukaryotes, and is postulated to be involved in multiple trafficking pathways from late endosomes. The machinery that synthesizes this lipid includes the PI3P 5'-kinase Fab1/PIKfyve and a regulatory ..
  31. Functional and Structural Studies of CD74 Activation
    Elias Lolis; Fiscal Year: 2010
    ..invariant chain that transports class II from the endoplasmic reticulum to vesicles which fuse with endosomes. The physiological role for the presence of CD74 on the cell surface is not known...
  32. NIR Light-Activated Nanoparticles for Drug and Gene Delivery
    JOSEPH ANTHONY ZASADZINSKI; Fiscal Year: 2013
    ..friendly NIR light energy absorbed by the HGN to heat, creating unstable microbubbles that mechanically rupture endosomes and release siRNA to the cytosol within seconds...
  33. The Regulation of Plasma Membrane Estrogen Receptor-a Levels by Endocytosis
    Reymundo Dominguez; Fiscal Year: 2012
    ..a finding we corroborated in cortical neurons by showing internalization of membrane-impermeable estradiol via endosomes. (2) Estradiol treatment of primary hippocampal and cortical neurons (a) rapidly initiates GRK2 (G protein-..
  34. Understanding the early and late endosomal TLR9-mediated responses to viral DNA.
    Yong Jun Liu; Fiscal Year: 2013
    ..Engagement of TLR9 by CpG-A oligodeoxynucleotide (ODN) in the early endosomes preferentially triggers the TRAF3/IRAK1/IKK1/PI3K/IRF7 signal cascade leading to type 1 IFN responses, whereas ..
  35. Role of Mechanisms of Efflux in Manganese Homeostasis and Detoxification
    Somshuvra Mukhopadhyay; Fiscal Year: 2013
    ..the Golgi requires SPCA1, a Golgi-localized Ca/Mn pump and that SPCA1 rapidly traffics between the Golgi and endosomes. As subcellular trafficking of ion pumps regulate their activity, our first aim is to elucidate the role of ..
  36. The Role of Cytoplasmic Dynein in Axonal Transport
    KEVIN K PFISTER; Fiscal Year: 2011
    ..is the motor for microtubule-based fast transport of various membranous organelles, including mitochondria, endosomes, and viruses in the retrograde direction from the axon terminus to the cell body...
  37. Proton-coupled folate/antifolate transport
    ISRAEL DAVID GOLDMAN; Fiscal Year: 2013
    ..PCFT also appears to provide an export route for folates from acidified endosomes consistent with a role in folate receptor - mediated transport...
  38. Antigen Binding, Crosslinking and Transmembrane Signaling
    David A Holowka; Fiscal Year: 2013
    ..We established the participation of Ca2+ mobilization in IgE-receptor stimulated trafficking from recycling endosomes, and in Aim 2 we will test the role of this process in stimulated cytokine secretion important for adaptive ..
  39. Epithelial Sodium Channel Trafficking
    Peter M Snyder; Fiscal Year: 2013
    ..by which E3 ubiquitin ligases regulate ENaC trafficking, investigate mechanisms that regulate ENaC sorting in endosomes, and investigate the role of lysine acetylation in ENaC trafficking...
  40. Regulation of Alcohol-induced Fatty Liver Disease by Lipid Droplet Trafficking
    MARK A MC NIVEN; Fiscal Year: 2010
    ..that chronic ethanol exposure alters the normal vesicle trafficking between lipid droplets and Golgi, endosomes, and endoplasmic reticulum, leading to fatty liver and subsequently hepatocellular damage...
  41. RAMP Mediated Gene Delivery
    Kevin G Rice; Fiscal Year: 2012
    ..to form polyplexes that target hepatocytes in vivo and undergo a glutathione triggered release of melittin in endosomes. This proposal is innovative in its development of a means to systematically vary the composition, sequence and ..
  42. PI(3,5)P2 synthesis in AMPA receptor trafficking and synaptic function
    AMBER JOYCE MCCARTNEY; Fiscal Year: 2012
    ..PI3P by phosphoinositide 5-kinase (PIKfyve), and is thought to be critical for the transition from early to late endosomes. My central hypothesis is that PI(3,5)P2 synthesis is a major intracellular signal that determines postendocytic ..
  43. REGULATION OF SIGNALING BY CELL-SURFACE PROTEOLYSIS
    Nigel W Bunnett; Fiscal Year: 2010
    ..Aim 3 examines the hypothesis that endosomes are platforms for sustained PAR inflammatory signaling and that endosomal proteases play a critical role in ..
  44. ADHESION JUNCTION REGULATION IN NORMAL AND DISEASED SKIN
    David S Rubenstein; Fiscal Year: 2012
    ..include (i) EGFR activation/phosphorylation, (ii) endocytosis of dsg3, plakoglobin and EGFR into early endosomes, (iii) and HSP27 phosphorylation...
  45. Function of SHP-1 in oligodendrocytes
    Paul T Massa; Fiscal Year: 2013
    ..sources of superoxide production in oligodendrocytes including ROS-producing complexes localized within early endosomes and mitochondria that are controlled by SHP-1...
  46. MOLECULAR STUDIES OF SELECTIVE PROTEIN TRANSPORT
    Gregory S Payne; Fiscal Year: 2013
    ..membrane proteins into the endocytic pathway and sorting proteins between the trans Golgi network (TGN) and endosomes. These ccv-mediated pathways are fundamental, conserved elements of eukaryotic cells;pathway defects can cause ..
  47. Cellular Mechanisms of Action of Mineralocorticoid Hormones
    John P Johnson; Fiscal Year: 2013
    ..and determine what specific aldosterone-regulated proteins control the activity and expression of ENaC in endosomes and exocyst compartments to support apical translocation of ENaC in Na+ reabsorptive states...
  48. Synthetic Cell Surface Receptors for Anticancer Drug Delivery
    Blake Peterson; Fiscal Year: 2012
    ..pathway occupied by the transferrin receptor involves rapid cycling between the cell surface and early/recycling endosomes, delivers transferrin to every cell of vertebrate animals, and represents a remarkable natural delivery vehicle...
  49. Macrophage Produced Phospholipid Transfer Protein (PLTP)
    Linda K Curtiss; Fiscal Year: 2012
    ..disrupts TLR2/1 plasma membrane dimerization and signaling, but not preformed TLR3 dimer mediated signaling in endosomes. We will assess the anti-inflammatory effects of MF PLTP on TLR2/1 and TLR3 signaling in PLTP- /-LDLr-/- mice ..
  50. Viral recognition by plasmacytoid dendritic cell
    Akiko Iwasaki; Fiscal Year: 2013
    ..by which AP-3 complex mediates trafficking of endosomal TLRs in pDCs and define the cell biology of IFN- endosomes. In the second Aim, we propose to identify the phosphoinositide-mediated signaling mechanism in pDCs...
  51. CpG-siRNA Conjugates to Target Acute Myeloid Leukemia
    Marcin Kortylewski; Fiscal Year: 2013
    ..the siRNA molecules equipped with a CpG moiety, are rapidly internalized by target cells and localized into endosomes in the absence of any transfection or packaging reagents...
  52. Molecular Regulation of Fusion: Voltage Dependence and Local Physical Interaction
    Fredric S Cohen; Fiscal Year: 2013
    ..All viruses that contain class II or class III fusion proteins (and some with class I) fuse from within endosomes. For these viruses, the endosome is the initial site of infection...
  53. Lipid Rafts in Adenoviral Ocular Pathogenesis
    Jaya Rajaiya; Fiscal Year: 2013
    ..Lipid rafts, including caveolin containing endosomes that bud off from the cell membrane during viral internalization, and signaling molecules associated with them, ..
  54. Intersectin Links Amyloidogenic Processes in Alzheimer Disease and Down Syndrome
    Jessica O Wilson; Fiscal Year: 2013
    ..by progressive dementia and the presence of beta-amyloid (A-beta) plaques, neurofibrillary tangles, and enlarged endosomes in the brain...
  55. Autophagy in Antiviral Immunity
    Akiko Iwasaki; Fiscal Year: 2013
    ..In professional viral sensors, the plasmacytoid dendritic cells (pDC), recognition of viruses occurs in the endosomes via Toll-like receptors (TLR) 7 and 9...
  56. Targeted Polymer Micelles for Treatment of Metastatic Melanoma
    Kevin N Sill; Fiscal Year: 2013
    ..pH, but releases the drug in response to lower pH environments, such as areas surrounding a tumor or within endosomes. Taxanes are a widely prescribed family of chemotherapeutic agents, but their efficacy is limited by systemic ..
  57. RECEPTOR MEDIATED ENDOCYTOSIS IN ALVEOLAR MACROPHAGES
    Diane McVey Ward; Fiscal Year: 2012
    ..and proteomic approaches combined with our ability to isolate highly pure populations of lysosomes and endosomes at specific stages of maturation to determine proteins involved in endosome maturation and regulation of ..
  58. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ..It is well established that Arfs promote the assembly of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly understood...
  59. Chemical Genetics of Iron Transport
    Marianne Wessling-Resnick; Fiscal Year: 2013
    ..that functions in dietary iron absorption across the apical membrane of enterocytes and after iron delivery into endosomes by the transferrin-mediated pathway...
  60. Functional Relevance of CFTR Trafficking in Vivo to Intestinal Disease
    Nadia A Ameen; Fiscal Year: 2013
    ..Unlike other epithelia that express CFTR only on the BBM, enterocytes express CFTR both on the BBM and subapical endosomes. The BBM fraction maintains basal intestinal fluidity by constitutive recycling of CFTR...
  61. Theranostic nanoparticles to enhance morpholino delivery to the liver for suppres
    DAVID PETER CORMODE; Fiscal Year: 2011
    ..used will possess the following features: morpholinos attached using a dithiol bond that will be cleaved in endosomes;a polymer coating that can disrupt endosomes for morpholino release into the cytoplasm;PEG chains to allow a ..
  62. Structural and Functional Specificity of Rab GTPases
    Guangpu Li; Fiscal Year: 2013
    ..This project is focused on two closely related Rab GTPases (Rab22 and Rab5) that are localized in early endosomes and regulate endosomal sorting and endocytosis...
  63. ARF BINDING IN THE ACTION OF CHOLERA AND E COLI TOXINS
    Richard Kahn; Fiscal Year: 2002
    ..Entry of the CT/LT toxins occurs through a "reverse secretory" pathway from endosomes to Golgi to ER and back out to Golgi, where A and B subunits dissociate and the active A subunit presumably ..
  64. ASSEMBLY OF NASCENT PLASMA LIPOPROTEINS BY HEPATOCYTES
    Robert Hamilton; Fiscal Year: 1991
    ..to interpret because the putative Golgi fractions used as a source of nascent lipoproteins were contaminated by endosomes containing remnant lipoproteins...
  65. Clathrin adaptor function at the trans Golgi network and endosomes
    Gregory Payne; Fiscal Year: 2007
    ..in the secretory pathway is the trans Golgi network (TGN), where proteins are directed to the plasma membrane, endosomes, and lysosomes...
  66. MECHANISM FOR CHARGED-RELATED NEPHROTOXICITY
    Darryl Peterson; Fiscal Year: 1993
    ..We have recently shown that endosomes containing cationic material fail to fuse with lysosomes during early stages of uptake, and remain sequestered ..
  67. Macrophage Foam Cells, Cathepsins and Cholesterol Efflux
    ALAN RICHARD TALL; Fiscal Year: 2010
    ..ABCA1 trafficks from the cell surface to late endosomes and co-operates with the Niemann-Pick C1 (NPC1) molecule in promoting efflux of cholesterol from endosomes to ..