Summary: Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN.

Top Publications

  1. pmc HDAC-6 interacts with and deacetylates tubulin and microtubules in vivo
    Yu Zhang
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    EMBO J 22:1168-79. 2003
  2. pmc Electrostatics of nanosystems: application to microtubules and the ribosome
    N A Baker
    Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:10037-41. 2001
  3. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
  4. doi Microtubule assembly during mitosis - from distinct origins to distinct functions?
    Sylvain Meunier
    Microtubule Function and Cell Division group, Cell and Developmental Biology Program, Centre for Genomic Regulation CRG and UPF, Dr Aiguader 88, 08003 Barcelona, Spain
    J Cell Sci 125:2805-14. 2012
  5. ncbi The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase
    Brian J North
    Department of Physiology, University of California, San Francisco, 94141, San Francisco, CA, USA
    Mol Cell 11:437-44. 2003
  6. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
  7. doi An EB1-binding motif acts as a microtubule tip localization signal
    Srinivas Honnappa
    Biomolecular Research, Structural Biology, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland
    Cell 138:366-76. 2009
  8. pmc The axonal transport of mitochondria
    William M Saxton
    Department of Molecular Cell and Developmental Biology, University of California, Cruz, CA 95060, USA
    J Cell Sci 125:2095-104. 2012
  9. pmc Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters
    Liedewij Laan
    FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
    Cell 148:502-14. 2012
  10. doi Microtubule assembly, organization and dynamics in axons and dendrites
    Cecilia Conde
    Laboratorio de Neurobiología Celular y Molecular, Instituto Investigación Médica Mercedes y Martín Ferreyra INIMEC CONICET, Friuli 2434, 5016 Cordoba, Argentina
    Nat Rev Neurosci 10:319-32. 2009

Detail Information

Publications417 found, 100 shown here

  1. pmc HDAC-6 interacts with and deacetylates tubulin and microtubules in vivo
    Yu Zhang
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    EMBO J 22:1168-79. 2003
    b>Microtubules are cylindrical cytoskeletal structures found in almost all eukaryotic cell types which are involved in a great variety of cellular processes...
  2. pmc Electrostatics of nanosystems: application to microtubules and the ribosome
    N A Baker
    Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 98:10037-41. 2001
    ..As a demonstration of this methodology, electrostatic potentials have been calculated for large microtubule and ribosome structures. The results point to the likely role of electrostatics in a variety of activities of these structures...
  3. pmc A mechanism linking extra centrosomes to chromosomal instability
    Neil J Ganem
    Howard Hughes Medical Institute, Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Nature 460:278-82. 2009
    ..These findings provide a direct mechanistic link between extra centrosomes and CIN, two common characteristics of solid tumours. We propose that this mechanism may be a common underlying cause of CIN in human cancer...
  4. doi Microtubule assembly during mitosis - from distinct origins to distinct functions?
    Sylvain Meunier
    Microtubule Function and Cell Division group, Cell and Developmental Biology Program, Centre for Genomic Regulation CRG and UPF, Dr Aiguader 88, 08003 Barcelona, Spain
    J Cell Sci 125:2805-14. 2012
    The mitotic spindle is structurally and functionally defined by its main component, the microtubules (MTs)...
  5. ncbi The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase
    Brian J North
    Department of Physiology, University of California, San Francisco, 94141, San Francisco, CA, USA
    Mol Cell 11:437-44. 2003
    ..a human ortholog of Sir2p, sirtuin type 2 (SIRT2), is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo...
  6. ncbi A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis
    Maxence V Nachury
    Genentech, Inc, South San Francisco, CA 94080, USA
    Cell 129:1201-13. 2007
    ..Conversely, preventing Rab8(GTP) production blocks ciliation in cells and yields characteristic BBS phenotypes in zebrafish. Our data reveal that BBS may be caused by defects in vesicular transport to the cilium...
  7. doi An EB1-binding motif acts as a microtubule tip localization signal
    Srinivas Honnappa
    Biomolecular Research, Structural Biology, Paul Scherrer Institut, 5232 Villigen PSI, Switzerland
    Cell 138:366-76. 2009
    b>Microtubules are filamentous polymers essential for cell viability...
  8. pmc The axonal transport of mitochondria
    William M Saxton
    Department of Molecular Cell and Developmental Biology, University of California, Cruz, CA 95060, USA
    J Cell Sci 125:2095-104. 2012
    ..fashion, has attracted special interest in recent years following the discovery of functional connections among microtubules, motor proteins and mitochondria, and their influences on neurodegenerative diseases...
  9. pmc Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters
    Liedewij Laan
    FOM Institute AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
    Cell 148:502-14. 2012
    ..Our results demonstrate the intrinsic ability of cortical microtubule-dynein interactions to regulate microtubule dynamics and drive positioning processes in living cells...
  10. doi Microtubule assembly, organization and dynamics in axons and dendrites
    Cecilia Conde
    Laboratorio de Neurobiología Celular y Molecular, Instituto Investigación Médica Mercedes y Martín Ferreyra INIMEC CONICET, Friuli 2434, 5016 Cordoba, Argentina
    Nat Rev Neurosci 10:319-32. 2009
    ..Far from being mere structural elements, microtubules are emerging as key determinants of neuronal polarity...
  11. ncbi Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons
    J G Gleeson
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 23:257-71. 1999
    ..DCX protein localization overlaps with microtubules in cultured primary cortical neurons, and this overlapping expression is disrupted by microtubule ..
  12. pmc Cytoskeletal forces span the nuclear envelope to coordinate meiotic chromosome pairing and synapsis
    Aya Sato
    Howard Hughes Medical Institute, Chevy Chase, MD 20815 USA
    Cell 139:907-19. 2009
    ..Disruption of microtubules inhibits chromosome pairing, indicating that these connections promote interhomolog interactions...
  13. pmc Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine
    Stefano Santaguida
    Department of Experimental Oncology, European Institute of Oncology, I 20139 Milan, Italy
    J Cell Biol 190:73-87. 2010
    ..They also shed light on the relationship between the error correction pathway and the spindle checkpoint and suggest that these processes are coregulated and are likely to share at least a subset of their catalytic machinery...
  14. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
    ..Based on these observations, we propose that HIV uses dynein and the microtubule network to facilitate the delivery of the viral genome to the nucleus of the cell during early postentry steps of the HIV life cycle...
  15. pmc The chromosomal passenger complex (CPC): from easy rider to the godfather of mitosis
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, ICB Michael Swann Building, King s Buildings Mayfield Road, Edinburgh EH9 3JR Scotland, UK
    Nat Rev Mol Cell Biol 13:789-803. 2012
    ..Our growing understanding of the CPC has seen it develop from a mere passenger riding on the chromosomes to one of the main controllers of mitosis...
  16. ncbi HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin
    Atsushi Iwata
    Department of Biological Sciences, Bio X Program, Stanford University, Stanford, California 94305 5430, USA
    J Biol Chem 280:40282-92. 2005
    ..These data suggest that HDAC6-dependent retrograde transport on microtubules is used by cells to increase the efficiency and selectivity of autophagic degradation.
  17. pmc Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface
    Julie P I Welburn
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 38:383-92. 2010
    Accurate chromosome segregation requires carefully regulated interactions between kinetochores and microtubules, but how plasticity is achieved to correct diverse attachment defects remains unclear...
  18. pmc The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development
    Aurélien Courtois
    Mammalian Development Laboratory, Max Planck Institute for Molecular Biomedicine, 48149 Munster, Germany
    J Cell Biol 198:357-70. 2012
    ..Overall, the transition from meiosis to mitosis progresses gradually throughout the preimplantation stage in the mouse embryo, thus providing a unique system to study the mechanism of centrosome biogenesis in vivo...
  19. pmc Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia
    Marie Schoumacher
    Unite Mixte de Recherche 144, Centre National de la Recherche Scientifique, Paris, Cedex 05, France
    J Cell Biol 189:541-56. 2010
    ..In contrast, elongation of invadopodia is mostly dependent on filopodial actin machinery. Moreover, intact microtubules and vimentin intermediate filament networks are required for further growth...
  20. pmc The trip of the tip: understanding the growth cone machinery
    Laura Anne Lowery
    Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:332-43. 2009
    ..The understanding of the functions and regulation of the vehicle and navigator provides new insights into the cell biology of growth cone guidance...
  21. pmc A FAM21-containing WASH complex regulates retromer-dependent sorting
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research and Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Dev Cell 17:699-711. 2009
    ..without WASH, retromer tubulation was exaggerated, supporting a model wherein WASH links retromer-mediated cargo containing tubules to microtubules for Golgi-directed trafficking and generates F-actin-driven force for tubule scission.
  22. pmc Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase
    Emily A Foley
    Laboratory of Chemistry and Cell Biology, Rockefeller University, 1230 York Avenue, New York, New York 10065, USA
    Nat Cell Biol 13:1265-71. 2011
    ..Our findings reveal that PP2A, an essential tumour suppressor, tunes the balance of phosphorylation to promote chromosome-spindle interactions during cell division...
  23. ncbi The molecular architecture of axonemes revealed by cryoelectron tomography
    Daniela Nicastro
    Laboratory for 3D Electron Microscopy of Cells, Department of Molecular, Cellular, and Developmental Biology, CB 347, University of Colorado, Boulder, CO 80309 0347, USA
    Science 313:944-8. 2006
    Eukaryotic flagella and cilia are built on a 9 + 2 array of microtubules plus >250 accessory proteins, forming a biological machine called the axoneme...
  24. doi Microtubule nucleation by γ-tubulin complexes
    Justin M Kollman
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143, USA
    Nat Rev Mol Cell Biol 12:709-21. 2011
    ..New experiments on γTuRC localization extend these insights, suggesting a direct link between its attachment at specific cellular sites and its activation...
  25. pmc In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation
    Akihisa Matsuyama
    Chemical Genetics Laboratory, Wako, Saitama 351 0198, Japan
    EMBO J 21:6820-31. 2002
    ..Our investigations suggest that HDAC6 controls the stability of a dynamic pool of microtubules. Indeed, we found that highly acetylated microtubules observed after TSA treatment exhibited delayed drug-..
  26. ncbi Visualization of cellulose synthase demonstrates functional association with microtubules
    Alexander R Paredez
    Department of Biological Sciences, Stanford University, 260 Panama Street, Stanford, CA 94305, USA
    Science 312:1491-5. 2006
    ..plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional...
  27. ncbi Feature point tracking and trajectory analysis for video imaging in cell biology
    I F Sbalzarini
    Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
    J Struct Biol 151:182-95. 2005
    ..of low-density lipoproteins in endosomes, motion of fluorescently labeled Adenovirus-2 particles along microtubules, and tracking of quantum dots on the plasma membrane of live cells...
  28. ncbi Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p
    Kenneth E Sawin
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3JR, United Kingdom
    Curr Biol 14:763-75. 2004
    ..These are the equatorial MTOC, which nucleates microtubules from the cell division site at the end of mitosis, and interphase MTOCs, which nucleate microtubules from ..
  29. doi Developmental patterning by mechanical signals in Arabidopsis
    Olivier Hamant
    INRA, Laboratoire de Reproduction et Développement des Plantes, 46 allee d Italie, 69364 Lyon Cedex 07, France
    Science 322:1650-5. 2008
  30. doi Microtubules and resistance to tubulin-binding agents
    Maria Kavallaris
    Children s Cancer Institute Australia for Medical Research, Randwick, NSW 2031, Australia
    Nat Rev Cancer 10:194-204. 2010
    b>Microtubules are dynamic structures composed of alpha-beta-tubulin heterodimers that are essential in cell division and are important targets for cancer drugs...
  31. ncbi Microtubule-organizing centres: a re-evaluation
    Jens Lüders
    Department of Biological Sciences, Stanford University, CA 94305, USA
    Nat Rev Mol Cell Biol 8:161-7. 2007
    The number, length, distribution and polarity of microtubules are largely controlled by microtubule-organizing centres, which nucleate and anchor microtubule minus ends in a process that requires gamma-tubulin...
  32. doi Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material
    Steffen Lawo
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada
    Nat Cell Biol 14:1148-58. 2012
    ..Globally, this work highlights the role of higher-order PCM organization in the regulation of centrosome assembly and function...
  33. pmc CDK5RAP2 stimulates microtubule nucleation by the gamma-tubulin ring complex
    Yuk Kwan Choi
    Department of Biochemistry, Hong Kong University of Science and Technology, Hong Kong, China
    J Cell Biol 191:1089-95. 2010
    ..microtubule nucleation by purified γ-TuRC in vitro and induces extensive, γ-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay...
  34. pmc CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content
    Branch Craige
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 190:927-40. 2010
    ..connectors, resulting in loss of attachment of the flagellar membrane to the transition zone microtubules. Biochemical analysis of isolated flagella revealed that the mutant flagella have abnormal protein content, ..
  35. doi Neuronal polarization: the cytoskeleton leads the way
    Michael Stiess
    Axonal Growth and Regeneration Group, Max Planck Institute of Neurobiology, Am Klopferspitz 18, Martinsried, Germany
    Dev Neurobiol 71:430-44. 2011
    ..First, the role of the actin cytoskeleton and microtubules in axon specification will be presented...
  36. pmc Mechanisms of chromosomal instability
    Sarah L Thompson
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Curr Biol 20:R285-95. 2010
    ..Understanding these mechanisms provides insight into the cellular consequences of CIN and reveals the possibility of exploiting CIN in cancer therapy...
  37. pmc The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments
    Jens C Schmidt
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Dev Cell 23:968-80. 2012
    ..chromosome segregation during mitosis, the macromolecular kinetochore must remain attached to depolymerizing microtubules, which drive chromosome movements...
  38. doi A Proteome-wide screen for mammalian SxIP motif-containing microtubule plus-end tracking proteins
    Kai Jiang
    Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    Curr Biol 22:1800-7. 2012
    ..Our study reveals the complexity of the mammalian +TIP interactome and provides a basis for investigating the molecular crosstalk between microtubule ends and other cellular structures...
  39. pmc Differential regulation of dynein and kinesin motor proteins by tau
    Ram Dixit
    Department of Physiology and Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 319:1086-9. 2008
    ..In neurons, microtubules are abundantly decorated with microtubule-associated proteins (MAPs) such as tau...
  40. pmc POM-POM2/cellulose synthase interacting1 is essential for the functional association of cellulose synthase and microtubules in Arabidopsis
    Martin Bringmann
    Max Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany
    Plant Cell 24:163-77. 2012
    ..Cellulose is synthesized at the plasma membrane, and the orientation of synthesis is guided by cortical microtubules; however, the guiding mechanism is currently unknown...
  41. doi Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions
    Carsten Janke
    Department of Signalling, Neurobiology and Cancer, Institut Curie, Bat 110, Centre Universitaire, 91405 Orsay Cedex, France
    Nat Rev Mol Cell Biol 12:773-86. 2011
    ..and biophysical experiments has provided attractive models that may explain the diverse functions of microtubules within cells and organisms...
  42. pmc Integrin-linked kinase controls microtubule dynamics required for plasma membrane targeting of caveolae
    Sara A Wickström
    Department of Molecular Medicine, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Dev Cell 19:574-88. 2010
    ..plasma membrane caveolae in vivo, which is due to impaired transport of caveolin-1-containing vesicles along microtubules (MT) to the plasma membrane...
  43. ncbi Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells
    Edgar R Gomes
    Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA
    Cell 121:451-63. 2005
    ..These results show that nuclear repositioning is an initial polarizing event in migrating cells and that the positions of the nucleus and the MTOC are established by separate regulatory pathways...
  44. pmc Mammalian end binding proteins control persistent microtubule growth
    Yulia Komarova
    Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL 60611, USA
    J Cell Biol 184:691-706. 2009
    ..This probably occurs through their action on microtubule ends, because catastrophe suppression does not require the EB domains needed for binding to known EB partners...
  45. pmc CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex
    Ka Wing Fong
    Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Mol Biol Cell 19:115-25. 2008
    ..Our findings suggest that centrosome malfunction due to the CDK5RAP2 mutations may underlie autosomal recessive primary microcephaly...
  46. pmc A protein factor essential for microtubule assembly
    M D Weingarten
    Proc Natl Acad Sci U S A 72:1858-62. 1975
    ..polypeptide chains (alpha and beta tubulin) with a molecular weight of 120,000, which will not assemble into microtubules in vitro. Addition of tau completely restores tubule-forming capacity...
  47. ncbi Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein)
    Tatiana Stepanova
    Medical Genetics Center Department of Cell Biology and Genetics, Erasmus University, 3000 DR Rotterdam, The Netherlands
    J Neurosci 23:2655-64. 2003
    ..CLIP-115, and EB1 (end-binding protein 1), have been shown to associate specifically with the ends of growing microtubules in non-neuronal cells, thereby regulating microtubule dynamics and the binding of microtubules to protein ..
  48. pmc The spatial arrangement of chromosomes during prometaphase facilitates spindle assembly
    Valentin Magidson
    Wadsworth Center, New York State Department of Health, Albany, NY 12201 509, USA
    Cell 146:555-67. 2011
    ..Our data demonstrate that unstable lateral interactions between kinetochores and microtubules dominate during early prometaphase...
  49. pmc The Ndc80 kinetochore complex forms oligomeric arrays along microtubules
    Gregory M Alushin
    Biophysics Graduate Group, University of California, Berkeley, California 94720, USA
    Nature 467:805-10. 2010
    ..present a subnanometre-resolution cryo-electron microscopy reconstruction of the human Ndc80 complex bound to microtubules, sufficient for precise docking of crystal structures of the component proteins...
  50. pmc Cyclin-dependent kinases regulate splice-specific targeting of dynamin-related protein 1 to microtubules
    Stefan Strack
    Department of Pharmacology, University of Iowa, Iowa City, IA 52246, USA
    J Cell Biol 201:1037-51. 2013
    ..Drp1-x01 stabilized and bundled microtubules and attenuated staurosporine-induced mitochondrial fragmentation and apoptosis...
  51. pmc Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells
    Ellen J Ezratty
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
    J Cell Biol 187:733-47. 2009
    Focal adhesion disassembly is regulated by microtubules (MTs) through an unknown mechanism that involves dynamin...
  52. ncbi HDAC6 is a microtubule-associated deacetylase
    Charlotte Hubbert
    Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710, USA
    Nature 417:455-8. 2002
    ..Acetylated alpha-tubulin is most abundant in stable microtubules but is absent from dynamic cellular structures such as neuronal growth cones and the leading edges of ..
  53. doi Unleashing formins to remodel the actin and microtubule cytoskeletons
    Melissa A Chesarone
    Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Nat Rev Mol Cell Biol 11:62-74. 2010
    ..The effects of formins on microtubules have also begun to be described, which places formins in a prime position to coordinate actin and microtubule ..
  54. doi Plus-end-tracking proteins and their interactions at microtubule ends
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus MC, P O Box 2040, 3000 CA Rotterdam, The Netherlands
    Curr Biol 20:R528-37. 2010
    b>Microtubules are cytoskeletal elements that are essential for a large number of intracellular processes, including mitosis, cell differentiation and migration, and vesicle transport...
  55. pmc Making the Auroras glow: regulation of Aurora A and B kinase function by interacting proteins
    Mar Carmena
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Edinburgh, Scotland, UK
    Curr Opin Cell Biol 21:796-805. 2009
    ..This review will focus on how interacting proteins make this functional diversity possible by targeting the kinases to different subcellular locations and regulating their activity...
  56. pmc Pausing of Golgi bodies on microtubules regulates secretion of cellulose synthase complexes in Arabidopsis
    Elizabeth Faris Crowell
    Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France
    Plant Cell 21:1141-54. 2009
    ..We find that CSC insertion in the plasma membrane is regulated by pauses of the Golgi apparatus along cortical microtubules. Our data support a model in which cortical microtubules not only guide the trajectories of CSCs in the plasma ..
  57. pmc A dynein loading zone for retrograde endosome motility at microtubule plus-ends
    J H Lenz
    Max Planck Institut fur terrestrische Mikrobiologie, Marburg, Germany
    EMBO J 25:2275-86. 2006
    In the fungus Ustilago maydis, early endosomes move bidirectionally along microtubules (MTs) and facilitate growth by local membrane recycling at the tip of the infectious hypha...
  58. pmc Tension directly stabilizes reconstituted kinetochore-microtubule attachments
    Bungo Akiyoshi
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Nature 468:576-9. 2010
    ..kinetochore-microtubule attachments, which come under tension as the result of opposing forces exerted by microtubules. Tension is thought to stabilize these bi-oriented attachments indirectly, by suppressing the destabilizing ..
  59. ncbi Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration
    Takashi Watanabe
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Dev Cell 7:871-83. 2004
  60. ncbi Histone deacetylase 6 inhibition compensates for the transport deficit in Huntington's disease by increasing tubulin acetylation
    Jim P Dompierre
    Institut Curie and Centre National de la Recherche Scientifique Unité Mixte de Recherche 146, Orsay, France
    J Neurosci 27:3571-83. 2007
    ..Our findings reveal that HDAC6 inhibition and acetylation at lysine 40 of alpha-tubulin may be therapeutic targets of interest in disorders such as HD in which intracellular transport is altered...
  61. ncbi Fast kinetics of Taxol binding to microtubules. Effects of solution variables and microtubule-associated proteins
    José Fernando Díaz
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, C Velázquez, 144 28006 Madrid, Spain
    J Biol Chem 278:8407-19. 2003
    The kinetics of Taxol association to and dissociation from stabilized microtubules has been measured by competition with the reference fluorescent derivative Flutax-1 (Diaz, J. F., Strobe, R., Engelborghs, Y., Souto, A. A., and Andreu, J...
  62. pmc The actin-binding ERM protein Moesin binds to and stabilizes microtubules at the cell cortex
    Sara Solinet
    Cellular Mechanisms of Morphogenesis during Mitosis and Cell Motility, Universite de Montreal, Montreal, Quebec H3C 3J7, Canada
    J Cell Biol 202:251-60. 2013
    ..In this paper, we show that the ERM protein Moesin directly binds to microtubules in vitro and stabilizes microtubules at the cell cortex in vivo...
  63. doi Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation
    Yasutaka Kakui
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0032, Japan
    Nat Cell Biol 15:786-96. 2013
    Tethering kinetochores at spindle poles facilitates their efficient capture and segregation by microtubules at mitotic onset in yeast...
  64. pmc Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization
    V Mennella
    Department of Biochemistry and Biophysics and the Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94158, USA
    Nat Cell Biol 14:1159-68. 2012
    ..Surrounding the centrioles, the pericentriolar material (PCM) provides a dynamic platform for nucleating microtubules. Although the importance of the PCM is established, its amorphous electron-dense nature has made it refractory ..
  65. doi Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome
    Takuma Misawa
    Laboratory of Host Defense, World Premier International Research Center Immunology Frontier Research Center, Osaka University, Osaka, Japan
    Nat Immunol 14:454-60. 2013
    ..Here we show that microtubules mediated assembly of the NLRP3 inflammasome...
  66. pmc CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast
    Valerie C Coffman
    Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 195:563-72. 2011
    ..In addition, we show that fission yeast has enough Dam1-DASH complex for ring formation around attached MTs. The results of this study suggest the need for significant revision of existing CEN/kinetochore architectural models...
  67. ncbi Microtubules facilitate autophagosome formation and fusion of autophagosomes with endosomes
    Robert Köchl
    Cancer Research UK, London Research Institute, Secretory Pathways Laboratory, 44 Lincoln s Inn Fields, London, WC2A 3PX, UK
    Traffic 7:129-45. 2006
    ..To determine the role of microtubules in autophagy, we used nocodazole and vinblastine to disrupt microtubules and independently measured formation ..
  68. pmc Ofd1, a human disease gene, regulates the length and distal structure of centrioles
    Veena Singla
    Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, CA 94158 2324, USA
    Dev Cell 18:410-24. 2010
    ..These long centrioles are structurally similar to normal centrioles but contain destabilized microtubules with abnormal posttranslational modifications...
  69. pmc Plus- and minus-end directed microtubule motors bind simultaneously to herpes simplex virus capsids using different inner tegument structures
    Kerstin Radtke
    Institute of Virology, Hannover Medical School, Hannover, Germany
    PLoS Pathog 6:e1000991. 2010
    ..Thus, these associated motors either engage in a tug-of-war or their activities are coordinately regulated to achieve net transport either to the nucleus during cell entry or to cytoplasmic membranes for envelopment during assembly...
  70. doi Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the dual leucine zipper kinase-c-Jun N-terminal kinase pathway
    Syu ichi Hirai
    Department of Molecular Biology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    J Neurosci 31:6468-80. 2011
    ..Together, these in vivo and in vitro observations indicate that the DLK-JNK pathway facilitates axon formation in neocortical neurons via stage-specific regulation of microtubule stability...
  71. pmc Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins
    Yi Hao
    Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Curr Biol 20:1809-18. 2010
    ..During mitosis, spindle poles align so the astral microtubules contact the lateral cortex...
  72. ncbi Dynein-dependent movement of autophagosomes mediates efficient encounters with lysosomes
    Shunsuke Kimura
    Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
    Cell Struct Funct 33:109-22. 2008
    ..Collectively, our data demonstrate the functional significance of autophagosome movement that enables effective delivery from the cytosol to lysosomes...
  73. pmc Multicolor super-resolution imaging with photo-switchable fluorescent probes
    Mark Bates
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Science 317:1749-53. 2007
    ..Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale...
  74. doi The 2.8 Å crystal structure of the dynein motor domain
    Takahide Kon
    Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Osaka 565 0871, Japan
    Nature 484:345-50. 2012
    ..We also identify a long-range allosteric communication pathway between the primary ATPase and the microtubule-binding sites. Our work provides a framework for understanding the mechanism of dynein-based motility...
  75. pmc Cellulose synthase interactive protein 1 (CSI1) links microtubules and cellulose synthase complexes
    Shundai Li
    Center for LignoCellulose Structure and Formation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 109:185-90. 2012
    ..complexes can be observed by live-cell imaging to move with trajectories that parallel the underlying cortical microtubules. Here we report that CESA interactive protein 1 (CSI1) is a microtubule-associated protein that bridges CESA ..
  76. pmc SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase
    Babet van der Vaart
    Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, Netherlands
    J Cell Biol 193:1083-99. 2011
    The ends of growing microtubules (MTs) accumulate a set of diverse factors known as MT plus end-tracking proteins (+TIPs), which control microtubule dynamics and organization...
  77. pmc Crystal structure of the dynein motor domain
    Andrew P Carter
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA
    Science 331:1159-65. 2011
    ..The arrangement of these elements provides clues as to how adenosine triphosphate-driven conformational changes might be transmitted across the motor domain...
  78. pmc Structural organization of the kinetochore-microtubule interface
    Jennifer G DeLuca
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, United States
    Curr Opin Cell Biol 24:48-56. 2012
    Successful mitosis depends on the stable, yet regulated attachment of chromosomes to spindle microtubules. The kinetochore, a large macromolecular structure assembled at sites of centromeric heterochromatin, is responsible for generating ..
  79. doi Microtubule inhibitors: Differentiating tubulin-inhibiting agents based on mechanisms of action, clinical activity, and resistance
    Edith A Perez
    Division of Hematology and Oncology, Mayo Clinic, Jacksonville, FL32224, USA
    Mol Cancer Ther 8:2086-95. 2009
    b>Microtubules are important cellular targets for anticancer therapy because of their key role in mitosis...
  80. ncbi Aggresomes, inclusion bodies and protein aggregation
    R R Kopito
    Dept of Biological Sciences, Stanford University, Stanford, CA 94305 5020, USA
    Trends Cell Biol 10:524-30. 2000
    ..recent studies suggest that, in animal cells, aggregated proteins are specifically delivered to inclusion bodies by dynein-dependent retrograde transport on microtubules. This microtubule-dependent inclusion body is called an aggresome.
  81. ncbi Molecular architecture of the kinetochore-microtubule interface
    Iain M Cheeseman
    Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Rev Mol Cell Biol 9:33-46. 2008
    ..genome during cell division in eukaryotes requires the kinetochore to link centromeric DNA to spindle microtubules. The kinetochore is composed of a number of conserved protein complexes that direct its specification and ..
  82. pmc Cytoskeletal dynamics in growth-cone steering
    Sara Geraldo
    The MRC Centre for Developmental Neurobiology, New Hunts House, Guy s Campus, King s College London, London SE1 1UL, UK
    J Cell Sci 122:3595-604. 2009
    Interactions between dynamic microtubules and actin filaments are essential to a wide range of cell biological processes including cell division, motility and morphogenesis...
  83. ncbi CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta
    Gideon Lansbergen
    MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
    Dev Cell 11:21-32. 2006
    ..and its recruitment to the cell cortex is influenced by PI3 kinase activity but does not require intact microtubules. Cortical clusters of LL5beta and ELKS do not overlap with focal adhesions but often form in their vicinity and ..
  84. ncbi Experimental and theoretical study of mitotic spindle orientation
    Manuel Thery
    Institut Curie, CNRS UMR144, Compartimentation et Dynamique Cellulaire, 26 Rue d Ulm, 75248 Paris, France
    Nature 447:493-6. 2007
    ..can be understood as the result of the action of cortical force generators, which interact with spindle microtubules and are activated by cortical cues...
  85. pmc Ligands for clathrin-mediated endocytosis are differentially sorted into distinct populations of early endosomes
    Melike Lakadamyali
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA
    Cell 124:997-1009. 2006
    ..endosomes are in fact comprised of two distinct populations: a dynamic population that is highly mobile on microtubules and matures rapidly toward late endosomes and a static population that matures much more slowly...
  86. doi The cytoskeleton and cancer
    Alan Hall
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Cancer Metastasis Rev 28:5-14. 2009
    ..These are all aspects of cell behavior than often go awry in cancer. This volume brings together those interested in understanding the contribution of the actin and microtubule cytoskeletons to the cell biology of cancer...
  87. doi Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts
    Wenxiang Meng
    RIKEN Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Cell 135:948-59. 2008
    Epithelial cells contain noncentrosomal microtubules (MTs), whose minus ends are oriented apically...
  88. pmc The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes rapid ciliogenesis and efficient mechanosensation
    Toshinobu Shida
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305 5345, USA
    Proc Natl Acad Sci U S A 107:21517-22. 2010
    Long-lived microtubules found in ciliary axonemes, neuronal processes, and migrating cells are marked by α-tubulin acetylation on lysine 40, a modification that takes place inside the microtubule lumen...
  89. pmc Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5
    T M Kapoor
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 150:975-88. 2000
    ..Chromosomes in monastrol-treated cells frequently have both sister kinetochores attached to microtubules extending to the center of the monoaster (syntelic orientation)...
  90. ncbi Microtubules as a target for anticancer drugs
    Mary Ann Jordan
    University of California Santa Barbara, Santa Barbara, California 93106, USA
    Nat Rev Cancer 4:253-65. 2004
  91. doi Arabidopsis cortical microtubules position cellulose synthase delivery to the plasma membrane and interact with cellulose synthase trafficking compartments
    Ryan Gutierrez
    Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA
    Nat Cell Biol 11:797-806. 2009
    ..Here we provide evidence that cortical microtubules also position the delivery of CESA complexes to the plasma membrane and interact with small CESA-containing ..
  92. ncbi Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing action on cell morphogenesis
    Ying Fu
    Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA
    Cell 120:687-700. 2005
    ..Meanwhile, ROP2 inactivates another target RIC1, whose activity promotes well-ordered cortical microtubules. RIC1-dependent microtubule organization not only locally inhibits outgrowth but in turn suppresses ROP2 ..
  93. ncbi 2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF
    Nicola J Mabjeesh
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Cell 3:363-75. 2003
    ..we report that 2ME2 inhibits tumor growth and angiogenesis at concentrations that efficiently disrupt tumor microtubules (MTs) in vivo...
  94. ncbi The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle
    Y Gachet
    Divisions of Biochemistry and Immunology, St George s Hospital Medical School, London, UK
    J Cell Sci 112:1257-71. 1999
    ..Here, we present evidence that P23 has properties of a tubulin binding protein that associates with microtubules in a cell cycle-dependent manner...
  95. ncbi Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division
    B Feierbach
    Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 W 168th Street, New York, NY 10032, USA
    Curr Biol 11:1656-65. 2001
    ..Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons...
  96. ncbi Post-translational modifications regulate microtubule function
    Stefan Westermann
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Rev Mol Cell Biol 4:938-47. 2003
  97. pmc Microtubule actin cross-linking factor (MACF): a hybrid of dystonin and dystrophin that can interact with the actin and microtubule cytoskeletons
    C L Leung
    Department of Pathology and Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    J Cell Biol 147:1275-86. 1999
    ..More importantly, we found that the COOH-terminal domain of mACF7 interacts with and stabilizes microtubules. In transfected cells full-length mACF7 can associate not only with actin but also with microtubules...
  98. doi Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments
    Julien Guizetti
    Institute of Biochemistry, Department of Biology, Swiss Federal Institute of Technology Zurich ETHZ, Schafmattstrasse 18, Zurich, Switzerland
    Science 331:1616-20. 2011
    ..Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site...
  99. pmc MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function
    Thomas Metzger
    Program in Developmental Biology, Sloan Kettering Institute, New York, New York 10065, USA
    Nature 484:120-4. 2012
    ..We conclude that improper nuclear positioning contributes to muscle dysfunction in a cell-autonomous fashion...
  100. pmc Structured illumination of the interface between centriole and peri-centriolar material
    Jingyan Fu
    Cancer Research UK Cell Cycle Genetics Group, Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Open Biol 2:120104. 2012
    ..closely associated with interphase centrioles in two tube-like layers: an inner layer occupied by centriolar microtubules, Sas-4, Spd-2 and Polo kinase; and an outer layer comprising Pericentrin-like protein (Dplp), Asterless (Asl) ..
  101. pmc Tension sensing by Aurora B kinase is independent of survivin-based centromere localization
    Christopher S Campbell
    Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92037, USA
    Nature 497:118-21. 2013
    ..6). Unlike wild-type Sli15, truncated Sli15 localizes to pre-anaphase spindle microtubules. Premature targeting of full-length Sli15 to microtubules by preventing Cdk1 (also known as Cdc28) ..

Research Grants70

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..studies will be conducted to measure interactions between -Syn and microtubule binding proteins [MAPs, tau] and microtubules [tubulin]...
  3. Dynamics of Kinesins, Dyneins, and Myosins
    Paul R Selvin; Fiscal Year: 2010
    ..g., no actin, no microtubules, no intermediate filaments..., to show how melanosomes make the transition between one motor system and another...
  4. Persisting Grand Mal and Myoclonic Seizures in JME including Veterans
    Antonio V Delgado-Escueta; Fiscal Year: 2013
    ..2 mutant. (Aim 3c) Dissect Defhc1.1 by deletion analysis to identify the portion of the protein that binds to microtubules, and confirm that it is also the same binding sit in Defhc1.2...
    Quentin J Baca; Fiscal Year: 2010
    ..experimentally examine associations of the CD45 receptor with other cytoskeletal elements such as actin and microtubules to test their role in regulation of receptor mobility...
  6. AAA ATPases linking mitochondria with microtubule processing in flies and yeast
    Karen G Hales; Fiscal Year: 2012
    ..and biochemically the interactions between these mitochondrial AAA ATPases (in both flies and yeast) and microtubules and other structures...
  7. Assembly and Maintenance of Centromeres in Filamentous Fungi
    Michael Freitag; Fiscal Year: 2013
    ..provided by applicant): Centromeres form the foundation of kinetochores, the attachment points for spindle microtubules that transport chromosomes into daughter nuclei during nuclear division...
  8. The Effects of Ethanol on Microtubules during Mouse Pre-implantation Development
    Yixian Zheng; Fiscal Year: 2012
  9. Centrosomin and Centrosomes in Cell Division
    Timothy L Megraw; Fiscal Year: 2012
    ..are the major microtubule-organizing centers (MTOCs) in animal cells and are required for efficient assembly of microtubules into the bipolar spindle apparatus at mitosis...
  10. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2013
    ..LTP), a robust cellular model for learning, is exploited to investigate subcellular components including microtubules for transport;smooth endoplasmic reticulum for calcium regulation and protein trafficking;polyribosomes, Golgi ..
  11. Molecular and cellular mechanism of Microcephaly
    Arnold Kriegstein; Fiscal Year: 2013
    ..Furthermore, the centrosome is the primary anchor for microtubules, enabling the differentiating neuron to initiate and extend an axon, a key process of neuron differentiation...
  12. Keratin Biology and Disease Aspects in Simple Epithelia
    Bishr Omary; Fiscal Year: 2013
    ..IF proteins, one of the three major cytoskeletal protein families that include actin microfilaments and tubulin microtubules, play important physiologic and disease-related roles whose significance is rapidly unfolding...
  13. Imaging retinal astrocytes, ganglion cells and axonal transport in vivo
    Brad Fortune; Fiscal Year: 2012
    ..Similarly, there is evidence to suggest that RGC axonal cytoskeletal components, specifically microtubules, are disrupted during the earliest stages of response to experimental injuries such as axotomy and experimental ..
  14. Distance-Dependent Structure and Function of Neuronal Dendrites
    Kristen M Harris; Fiscal Year: 2010
    ..will be investigated along dendrites and into dendritic spines that host excitory synapses, including microtubules for transport, polyribosomes for protein synthesis, Golgi apparatus for posttranslational modifications, ..
    Susan K Dutcher; Fiscal Year: 2013
    ..Additionally, MTOCs are the focal point for cytoplasmic microtubules, and are often near the nucleus of the cell...
  16. HDAC6: a target for regeneration following injury in the nervous system
    BRETT CAMERON LANGLEY; Fiscal Year: 2013
    ..We have found that inhibiting histone deacetylase-6 (HDAC6), an enzyme responsible for the modulation of microtubules, appears to be an excellent, non-toxic therapeutic strategy for promoting regeneration in cell culture models...
  17. Cellular and molecular mechanisms of gp120 neurotoxicity
    Italo Mocchetti; Fiscal Year: 2012
    ..Endocytosis of gp120 is followed by axonal transport through microtubules. The length of axons and survival rate of neurons that do not internalize gp120 is significantly greater when ..
  18. Function of Microtubule Plus-End-Tracking Proteins in the Neuronal Growth Cone
    Laura Anne Lowery; Fiscal Year: 2013
    ..of +TIP function, have led to the working model that, within the growth cone, XMAP215 and Maskin interact with microtubules (MTs) in a functionally-distinct manner compared to CLASP, and that Abl signaling leads to differences in the ..
  19. Cisplatin Resistance mediated by copper export pathways
    Stephen B Howell; Fiscal Year: 2013
    ..the loading of Cu onto ceruloplasmin by ATP7B, and dynactin that links vesicles to the motor protein dynein and microtubules and whose p62 subunit is known to interact with ATP7B in a Cu-dependent manner...
  20. Mechanisms of pox virus motility and release
    Daniel Kalman; Fiscal Year: 2012
    ..enter mammalian cells, replicate extranuclearly, and produce virions that travel to the cell surface along microtubules, fuse with the plasma membrane, and egress away from infected cells towards apposing cells on actin-filled ..
  21. Endoplasmic reticulum structure and dynamics in breast cancer cell metastasis and
    JASON EDWARD LEE; Fiscal Year: 2013
    ..Additionally, ER dynamics along microtubules (MTs) have been characterized as tip- attachment complex (TAC) dynamics and ER sliding...
  22. Meiotic spindle formation in Drosophila females
    Kim S McKim; Fiscal Year: 2010
    During the first meiotic division, homologous chromosomes linked by chiasmata interact with the spindle microtubules and segregate to opposite poles...
  23. Microtubule dynamics during cell polarity and migration
    Torsten Wittmann; Fiscal Year: 2012
    ..The overall objective of this proposal is to determine the mechanisms by which microtubules function during establishment and maintenance of planar polarity and directed sheet migration of epithelial ..
  24. Developmental Regulation of the Cell Cycle in Drosophila
    LAURA ANNE LEE; Fiscal Year: 2013
    ..data suggest that dynein anchored on the nuclear surface of spermatocytes may preferentially bind to microtubules that are post-translationally modified by acetylation...
  25. Analyses of two RNA helicases that affect intercellular transport via plasmodesma
    Patricia C Zambryski; Fiscal Year: 2012
    ..2. Three types of analyses will characterize ISE1. A. Based on the fact that ISE1 also localizes to microtubules, we will perform in depth analyses of ISE1 microtubule association and PD targeting. B...
  26. Receptor Tyrosine Kinase Sorting and Transactivation in Endosomes
    MARK LINDSAY GRIMES; Fiscal Year: 2010
    ..two receptors away from one another involves dynamic interactions between detergent-insoluble lipid rafts and microtubules. We propose to analyze interactions between TrkA, p75NTR and microtubules using fractionation of organelles and ..
  27. Cell Adhesion and Cytoskeletal Dynamics in Skin
    Elaine Fuchs; Fiscal Year: 2013
    ..Elucidating how microtubules (MTs) link to AJs is important for understanding how the stratified epidermis maintains a single layer of ..
    Elizabeth C Engle; Fiscal Year: 2013
    ..TUBB3 substitutions reside at putative kinesin interaction sites on [unreadable]-tubulin, and we found that microtubules from knock-in mice had decreased interactions with the CFEOM1 gene product, Kif21a...
  29. Cytoskeletal Regulation During Growth Cone Migration and Axon Guidance
    Frank B Gertler; Fiscal Year: 2012
    ..may act to coordinate F-actin:MT interactions, and that the TRIM9 protein interacts with both Ena/VASP and microtubules;TRIM9 is also implicated in the control of axon navigation...
  30. A zebrafish model for neural regeneration
    ALLISON F ROSENBERG; Fiscal Year: 2012
    ..I will also investigate when axonal microtubules degenerate, a critical commitment step in axonal degeneration...
  31. Microtubule-associated Rac
    Anna Birukova; Fiscal Year: 2013
    ..These results have led us to a hypothesis that microtubules may mediate barrier-protective effects of HGF and attenuate acute pulmonary EC dysfunction via MT-associated ..
  32. Homolog orientation and segregation in acentrosomal meiosis
    Kim S McKim; Fiscal Year: 2013
    ..applicant): During the first meiotic division, homologous chromosomes linked by chiasmata interact with spindle microtubules and segregate to opposite poles...
  33. Integrating cell sorting and tissue shaping mechanisms during cornea maturation
    Philip M Iannaccone; Fiscal Year: 2010
    ..we will utilize modern advancements in both genetic and biomedical technologies to examine the role of microtubules in affecting epithelial cell segregation behaviors...
  34. Structure and mechanism of the dynein motor
    Ronald D Vale; Fiscal Year: 2013
    ..form of dynein was found to move numerous cargos (membrane organelles, the nucleus, mRNAs, proteins, microtubules, and viruses) towards the microtubule minus end in most eukaryotic cells...
    JOANNA LOUISE GRODEN; Fiscal Year: 2010
    ..mechanisms of growth control and differentiation is less emphasized, but includes effects on cell adhesion, microtubules and chromosome dynamics (Nathke et al., 1996;Kaplan et al., 2001;Mogensen era/., 2002;Bienz and Hamada, 2004)...
  36. The regulation of ciliary motility by an axonemal PP2A phosphatase
    Candice Elam; Fiscal Year: 2011
    ..and electron microscopy to test the hypothesis that the B-subunit of PP2A is localized to the outer doublet microtubules in position to regulate inner arm 11 dynein, the dynein that appears to be critical for control of microtubule ..
  37. Cytoplasmic trafficking of non-viral gene therapy vectors
    David A Dean; Fiscal Year: 2012
    ..shown that this cyclic stretch reorganizes the cytoskeleton and increases the numbers of stable, acetylated microtubules by a mechanism mediated by inhibition of the cytoplasmic histone deacetylase HDAC6, whose main target is 1-..
  38. Intra- and Inter-Neuronal Viral Trafficking
    Glenn F Rall; Fiscal Year: 2012
    ..In addition, interaction with cellular motor proteins that govern movement along microtubules will be determined...
  39. Regulation of Borealin Function by Mitotic Phosphorylation
    William Taylor; Fiscal Year: 2009
    ..passenger proteins, INCENP, Survivin, Aurora B kinase and Borealin form a complex that binds to centromeres and microtubules and coordinates chromosome segregation and division of the cell during cytokinesis...
  40. Gap Junction Trafficking to and within the Plasma Membrane
    ROBIN MARK SHAW; Fiscal Year: 2013
    ..capture of cortical membrane, preventing delivery of connexons to the plasma membrane;that actin helps microtubules position and target ion channels to specific regions of the cortical membrane, and that lateral diffusion of ..
    William J Snell; Fiscal Year: 2012
    ..Moreover, we found that a protein that disassembles microtubules, a depolymerizing kinesin, in the cell body is phosphorylated and transported into flagella as microtubule ..
  42. Mechanisms of radiosensitization by 2-methoxyestradiol in prostate cancer models
    James M Larner; Fiscal Year: 2012
    ..To address the initial events of 2-ME action, the interaction between microtubules and MAPK will be examined by immunofluorescence microscopy and other quantitative techniques to test whether 2-..
  43. Molecular Mechanisms of Cytoskeletal Regulators
    Kevin C Slep; Fiscal Year: 2013
    ..b>Microtubules are polymers of a[unreadable]-tubulin heterodimers...
  44. Pathogenesis of TSC2-Related Tumors
    Raymond Yeung; Fiscal Year: 2012
    ..Since microtubules play an important role in long-distant transport, cell polarity, migration and cytokinesis, we hypothesize that ..
  45. Identification of a protein that elicits immune-mediated neuronal dysfunction
    Bonnie N Dittel; Fiscal Year: 2010
    ..and survival is dependent upon fast axonal transport of neurotransmitters and growth factors in the axons along microtubules. Thus the destablization of microtubules puts the neuron at risk for death...
  46. Transporter Trafficking Mechanisms in Infectious Diarrhea
    Pradeep K Dudeja; Fiscal Year: 2013
    ..Aim 2 will investigate mechanisms underlying DRA trafficking in vitro, including the role of lipid rafts, microtubules and NHERF proteins in response to EPEC or EspGs...
  47. Regulation and Function of a Bacterial Cytoskeleton
    R Dyche Mullins; Fiscal Year: 2013
    ..Until recently, cytoskeletal polymers like actin filaments and microtubules were thought to be eukaryotic innovations, not present in bacteria...
  48. Age and molecular mechanisms contributing to aneuploidy in oocytes
    Richard M Schultz; Fiscal Year: 2013
    ..Another process that prevents errors is regulation of connections between kinetochores and spindle microtubules that results in a spindle with chromosomes correctly attached...
  49. Connexin Distribution in Physiological Versus Pathological Cardiac Hypertrophy
    Michael R Zile; Fiscal Year: 2013
    ..Major findings have been that 1) it is based both on increased tubulin, and thus microtubules, and on greater microtubule stability, 2) the major car- diac microtubule-stabilizing microtubule-associated ..
  50. Microtubule Stability in Lung Endothelium
    CRISTHIAAN D OCHOA ARENAS; Fiscal Year: 2012
    ..including reorganization of the cortical actin rim into stress fibers, and disassembly and reorganization of microtubules. Despite their importance in control of endothelial cell shape, relatively little is known about intracellular ..
    Mark J Czaja; Fiscal Year: 2013
    ..First, we will test the hypothesis that JNK2-dependent phosphorylation of stathmin stabilizes microtubules to block hepatocyte death from ROS and TNF...
  52. Regulation of cell division by mitotic kinases
    ..Forces exerted by spindle microtubules create tension across the centromere, which may regulate phosphorylation of a kinetochore substrate by ..
  53. Regulation of Endothelial Cell Branching Morphogenesis via MCAK-targted Control
    KENNETH ALBERT MYERS; Fiscal Year: 2013
    ..The transition from neuronal studies of microtubules and molecular motor proteins to high-resolution imaging in endothelial cells was intuitive...
  54. Novel Roles of Intracrine Angiotensin II in Proximal Tubule Cells
    Jia L Zhuo; Fiscal Year: 2013
    ..II is mediated by two major endocytic motifs of AT1a receptors in the cytoplasmic tail, and is regulated by the microtubules- and lipid rafts/caveolin-1 (CAV-1)-dependent mechanisms....
  55. Mechanisms of how nuclear envelope bridges link nuclei to the cytoskeleton.
    Daniel A Starr; Fiscal Year: 2013
    ..Specifically, the molecular mechanisms of how proteins are trafficked to the inner nuclear mem- brane, how microtubules and motors are coordinated to move nuclei, and how KASH and SUN proteins interact to connect cytoplasmic forces ..
  56. A Molecular View of Chromosome Recombination &Segregation in Eukaryotic Meiosis
    KEVIN DANIEL CORBETT; Fiscal Year: 2013
    ..complex, which binds chromosomes'kinetochores in meiosis I and modifies their attachments to spindle microtubules, to enable the proper orientation and segregation of homologous chromosomes...
    LISA MARIE LANDINO; Fiscal Year: 2011
    ..neurons containing neurofibrillary tangles, a lesion characteristic of Alzheimer's disease, the number of microtubules is reduced by 50%...
  58. Calcium regulation of flagellar motility
    Elizabeth F Smith; Fiscal Year: 2012
    ..Substantial data has contributed to a model in which the axonemal microtubules act as a scaffold for the assembly of molecules that form a signal transduction pathway that ultimately ..
  59. Polyphenolic Interventions for tau Pathogenesis in Alzheimers Models
    SALLY ANN FRAUTSCHY; Fiscal Year: 2013
    ..2. Fisetin reduces monomeric and insoluble ptau and increases unphosphorylated tau and stabilizes microtubules;however it does not result in reduction of oligomers, and only causes modest improvements in synaptic indices ..
  60. Nox4 and Vascular Smooth Muscle Differentiation
    Kathy K Griendling; Fiscal Year: 2013
    ..the molecular mechanisms underlying Nox4/Poldip2-mediated regulation of the paxillin homologue Hic-5 and microtubules and their role in focal adhesion turnover and differentiation marker gene expression...
  61. Regulation of Chromosome Segregation
    SUSAN BIGGINS; Fiscal Year: 2013
    ..Chromosome segregation requires forces generated by spindle microtubules that are translated into chromosome movement through interactions with kinetochores, the highly conserved ..
  62. Kinetochore-Microtubule Interactions
    JOHN RICHARD MCINTOSH; Fiscal Year: 2012
    ..Proper mitosis, in turn, depends on correct interactions between kinetochores and spindle microtubules. We have developed cell biological and biophysical methods to study the interactions between isolated ..
    Clarence Chan; Fiscal Year: 1999
    ..He proposes to determine the location of the Ilp1 kinase; he suggests that it may be located on microtubules. Since the protein is present at relatively low abundance, he presents several alternative approaches, ..
    Linda Callahan; Fiscal Year: 2001
    ..It has been speculated that an inability of tau to stabilize microtubules in AD neurons results in a destabilatation of microtubule...
    Richard Luduena; Fiscal Year: 2000
    DESCRIPTION: Microtubules are cylindrical organelles which play critical roles in mitosis and other cell processes. Because of their role in mitosis, microtubules are the target of taxol, vinblastine and other anti-tumor drugs...
    Richard Vallee; Fiscal Year: 1991
    ..Preliminary studies have yielded a new procedure for isolating microtubules from this source which involves the use of taxol to promote assembly...
    KENNETH KOSIK; Fiscal Year: 1999
    ..proposal has three parts all of which focus on the transduction of either normal or pathological signals to the microtubules in Alzheimer's disease...
    KENNETH KOSIK; Fiscal Year: 2000
    ..This transition involves a highly dynamic interaction between microtubules and actin filaments in which dynamic microtubules penetrate the dense actin network in the growth cone lamella ..
  69. Tau phosphorylation and neurodegeneration
    Alejandra del C Alonso; Fiscal Year: 2009
    ..tau from either AD brain or generated in vitro show that this tau binds normal MAPs, disrupts microtubules, and self-assembles into PHFs/SFs (Alonso et al., 1994, 1996, 1997, 2001a, 2001b, 2004)...
    Michael Caplow; Fiscal Year: 2003
    b>Microtubules are dynamic polymers of the tubulin alpha/beta dimer that provide the tracks for the motor proteins dynein and kinesin and are, therefore, involved in intracellular transport and chromosome separation...