stress fibers

Summary

Summary: Bundles of actin filaments (MICROFILAMENTS) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.

Top Publications

  1. pmc Transforming growth factor-beta-induced mobilization of actin cytoskeleton requires signaling by small GTPases Cdc42 and RhoA
    Sofia Edlund
    Ludwig Institute for Cancer Research, Biomedical Center, S 751 24 Uppsala, Sweden
    Mol Biol Cell 13:902-14. 2002
  2. ncbi Hippo pathway regulation by cell morphology and stress fibers
    Ken Ichi Wada
    Department of Cell Fate Control, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Development 138:3907-14. 2011
  3. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
  4. ncbi Actin stress fibers--assembly, dynamics and biological roles
    Sari Tojkander
    Institute of Biotechnology, University of Helsinki, Finland
    J Cell Sci 125:1855-64. 2012
  5. pmc Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
    Jérôme M Goffin
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    J Cell Biol 172:259-68. 2006
  6. pmc Cyclic stress at mHz frequencies aligns fibroblasts in direction of zero strain
    Uta Faust
    Institute of Complex Systems, ICS 7, Biomechanics, Forschungszentrum Julich GmbH, Julich, Germany
    PLoS ONE 6:e28963. 2011
  7. pmc Cooperative effects of Rho and mechanical stretch on stress fiber organization
    Roland Kaunas
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 102:15895-900. 2005
  8. ncbi A molecular pathway for myosin II recruitment to stress fibers
    Sari Tojkander
    Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
    Curr Biol 21:539-50. 2011
  9. ncbi Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions
    Takeshi Kobayashi
    Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan
    Curr Opin Cell Biol 22:669-76. 2010
  10. ncbi Cell distribution of stress fibres in response to the geometry of the adhesive environment
    Manuel Thery
    Biologie du cycle cellulaire et de la motilité, UMR144, CNRS, Institut Curie, Paris, France
    Cell Motil Cytoskeleton 63:341-55. 2006

Detail Information

Publications239 found, 100 shown here

  1. pmc Transforming growth factor-beta-induced mobilization of actin cytoskeleton requires signaling by small GTPases Cdc42 and RhoA
    Sofia Edlund
    Ludwig Institute for Cancer Research, Biomedical Center, S 751 24 Uppsala, Sweden
    Mol Biol Cell 13:902-14. 2002
    ..Long-term stimulation with TGF-beta 1 resulted in an assembly of stress fibers; this response required both signaling via Cdc42 and RhoA, and Smad proteins...
  2. ncbi Hippo pathway regulation by cell morphology and stress fibers
    Ken Ichi Wada
    Department of Cell Fate Control, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Development 138:3907-14. 2011
    ..b>Stress fibers increased in response to expanded cell areas, and F-actin regulated Yap downstream of cell morphology...
  3. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..No actin stress fibers are seen in fibroblasts on soft surfaces, and the appearance of stress fibers is abrupt and complete at a ..
  4. ncbi Actin stress fibers--assembly, dynamics and biological roles
    Sari Tojkander
    Institute of Biotechnology, University of Helsinki, Finland
    J Cell Sci 125:1855-64. 2012
    ..b>Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have a central role in ..
  5. pmc Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers
    Jérôme M Goffin
    Laboratory of Cell Biophysics, Ecole Polytechnique Federale de Lausanne, CH 1015, Lausanne, Switzerland
    J Cell Biol 172:259-68. 2006
    ..We identify alpha-SMA as a mechanosensitive protein that is recruited to stress fibers under high tension...
  6. pmc Cyclic stress at mHz frequencies aligns fibroblasts in direction of zero strain
    Uta Faust
    Institute of Complex Systems, ICS 7, Biomechanics, Forschungszentrum Julich GmbH, Julich, Germany
    PLoS ONE 6:e28963. 2011
    ..Cyclic stretch increases the number of stress fibers and the coupling to adhesions...
  7. pmc Cooperative effects of Rho and mechanical stretch on stress fiber organization
    Roland Kaunas
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 102:15895-900. 2005
    The small GTPase Rho regulates the formation of actin stress fibers in adherent cells through activation of its effector proteins Rho-kinase and mDia...
  8. ncbi A molecular pathway for myosin II recruitment to stress fibers
    Sari Tojkander
    Institute of Biotechnology, University of Helsinki, Helsinki 00014, Finland
    Curr Biol 21:539-50. 2011
    ..In contrast, the mechanisms by which contractile actomyosin arrays such as stress fibers are generated in cells, and how myosin II is specifically recruited to these structures, are not known.
  9. ncbi Sensing substrate rigidity by mechanosensitive ion channels with stress fibers and focal adhesions
    Takeshi Kobayashi
    Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan
    Curr Opin Cell Biol 22:669-76. 2010
    ..To sense substrate rigidity, cells apply traction forces to cell-substrate adhesions via actin stress fibers (SFs) and measure mechanical responses of the substrate...
  10. ncbi Cell distribution of stress fibres in response to the geometry of the adhesive environment
    Manuel Thery
    Biologie du cycle cellulaire et de la motilité, UMR144, CNRS, Institut Curie, Paris, France
    Cell Motil Cytoskeleton 63:341-55. 2006
    ..We conclude that cells of a given area can generate the same total sum of tractional forces but that these tractional forces are differently spaced depending on the spatial distribution of its adherence contacts...
  11. pmc Stretch-induced actin remodeling requires targeting of zyxin to stress fibers and recruitment of actin regulators
    Laura M Hoffman
    Departments of Biology and Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Mol Biol Cell 23:1846-59. 2012
    Reinforcement of actin stress fibers in response to mechanical stimulation depends on a posttranslational mechanism that requires the LIM protein zyxin...
  12. pmc Adherens junctions connect stress fibres between adjacent endothelial cells
    Jaime Millan
    University College London, Ludwig Institute for Cancer Research and Department of Biochemistry and Molecular Biology, London WC1E6BT, UK
    BMC Biol 8:11. 2010
    ..However, in the endothelium, cell-cell junctions are highly dynamic and morphologically heterogeneous...
  13. pmc A critical role of tropomyosins in TGF-beta regulation of the actin cytoskeleton and cell motility in epithelial cells
    Andrei V Bakin
    Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA
    Mol Biol Cell 15:4682-94. 2004
    We have investigated transforming growth factor beta (TGF-beta)-mediated induction of actin stress fibers in normal and metastatic epithelial cells...
  14. pmc B-Raf acts via the ROCKII/LIMK/cofilin pathway to maintain actin stress fibers in fibroblasts
    Catrin A Pritchard
    Department of Biochemistry, University of Leicester, United Kingdom
    Mol Cell Biol 24:5937-52. 2004
    ..5-fold increase in cell migration. Consistent with these findings, the high level of actin stress fibers normally present in MEFs was considerably reduced following disruption of B-raf, and the F-actin content of B-..
  15. ncbi FHL3 is an actin-binding protein that regulates alpha-actinin-mediated actin bundling: FHL3 localizes to actin stress fibers and enhances cell spreading and stress fiber disassembly
    Imogen D Coghill
    Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Melbourne, Victoria, Australia
    J Biol Chem 278:24139-52. 2003
    ..the nucleus in C2C12 myoblasts and, following integrin engagement, exited the nucleus and localized to actin stress fibers and focal adhesions. In mature skeletal muscle FHL3 was found at the Z-line...
  16. pmc Stress fibers are generated by two distinct actin assembly mechanisms in motile cells
    Pirta Hotulainen
    Institute of Biotechnology, University of Helsinki, Helsinki FI 00014, Finland
    J Cell Biol 173:383-94. 2006
    b>Stress fibers play a central role in adhesion, motility, and morphogenesis of eukaryotic cells, but the mechanism of how these and other contractile actomyosin structures are generated is not known...
  17. ncbi Mechanosensing in actin stress fibers revealed by a close correlation between force and protein localization
    Julien Colombelli
    Cell Biology and Biophysics, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    J Cell Sci 122:1665-79. 2009
    ..cytoskeleton laser nanosurgery in cultured epithelial cells and fibroblasts, we show that the retraction of stress fibers (SFs) is restricted to the proximity of the cut and that new adhesions form at the retracting end...
  18. pmc Dynamic and structural signatures of lamellar actomyosin force generation
    Yvonne Aratyn-Schaus
    Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA
    Mol Biol Cell 22:1330-9. 2011
    ..Diverse lamellar actin organizations ranging from contractile lamellar networks to stress fibers are observed in adherent cells...
  19. pmc ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles
    Yoshihiko Shimizu
    Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo ku, Kyoto 606 8501, Japan
    J Cell Biol 168:941-53. 2005
    ..These results suggest that ROCK-I regulates closure of the eyelids and ventral body wall through organization of actomyosin bundles...
  20. pmc A zyxin-mediated mechanism for actin stress fiber maintenance and repair
    Mark A Smith
    Department of Biology, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84112, USA
    Dev Cell 19:365-76. 2010
    ..By imaging live cells expressing fluorescently tagged cytoskeletal proteins, we observed that actin stress fibers undergo local, acute, force-induced elongation and thinning events that compromise their stress transmission ..
  21. ncbi Abolishing myofibroblast arrhythmogeneicity by pharmacological ablation of α-smooth muscle actin containing stress fibers
    Christian Rosker
    Dept of Physiology, University of Bern, Buhlplatz 5, CH 3012 Bern, Switzerland
    Circ Res 109:1120-31. 2011
    ..So far, it is not known whether α-smooth muscle actin (α-SMA) containing stress fibers, the cytoskeletal components that set myofibroblasts apart from resident fibroblasts, are essential for ..
  22. pmc Assembly and reorientation of stress fibers drives morphological changes to endothelial cells exposed to shear stress
    Sabrena Noria
    Toronto General Research Institute, University Health Network, Toronto, Canada
    Am J Pathol 164:1211-23. 2004
    ..Actin assembly occurred primarily at the ends of stress fibers, which simultaneously reoriented to the shear axis, frequently fused with neighboring stress fibers, and ..
  23. pmc The tension mounts: stress fibers as force-generating mechanotransducers
    Keith Burridge
    Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 200:9-19. 2013
    b>Stress fibers (SFs) are often the most prominent cytoskeletal structures in cells growing in tissue culture...
  24. ncbi Mechanisms of force generation and transmission by myofibroblasts
    Boris Hinz
    Laboratory of Cell Biophysics, Swiss Federal Institute of Technology EPFL, Bâtiment SG AA B 143, CH 1015 Lausanne, Switzerland
    Curr Opin Biotechnol 14:538-46. 2003
    ..Force generation by the myofibroblast depends on the isometric contraction of stress fibers containing alpha-smooth muscle actin, and is mediated through Rho/Rho-kinase...
  25. pmc The mammalian formin FHOD1 is activated through phosphorylation by ROCK and mediates thrombin-induced stress fibre formation in endothelial cells
    Ryu Takeya
    Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
    EMBO J 27:618-28. 2008
    ....
  26. pmc RhoE function is regulated by ROCK I-mediated phosphorylation
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, UK
    EMBO J 24:1170-80. 2005
    ..This is the first demonstration of an endogenous Rho family member being phosphorylated in vivo and indicates that phosphorylation is an important mechanism to control the stability and function of this GTPase-deficient Rho protein...
  27. pmc Specification of actin filament function and molecular composition by tropomyosin isoforms
    Nicole S Bryce
    Oncology Research Unit, Department of Paediatrics and Child Health, University of Sydney, The Children s Hospital at Westmead, Australia
    Mol Biol Cell 14:1002-16. 2003
    ..hTm5(NM1) was able to recruit myosin II into stress fibers, which resulted in decreased lamellipodia and cellular migration...
  28. ncbi An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers
    Tea Vallenius
    Institute of Biotechnology and Genome Scale Biology Program, University of Helsinki, PO Box 56 Viikinkaari 9, 00014 University of Helsinki, Finland
    J Cell Sci 124:384-93. 2011
    ..Association of NUAK2 with MRIP increases MLC phosphorylation and promotes formation of stress fibers. This activity does not require the kinase activity of NUAK2 but is dependent on both MRIP and MYPT1, ..
  29. ncbi A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin
    Adi D Dubash
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill NC 27599, USA
    J Cell Sci 120:3989-98. 2007
    ..Knockdown of Lsc and LARG together significantly decreases RhoA activation and formation of stress fibers and focal adhesions downstream of fibronectin adhesion...
  30. ncbi PKCdelta regulates endothelial basal barrier function through modulation of RhoA GTPase activity
    Elizabeth O Harrington
    Pulmonary Vascular Research Laboratory, Providence VA Medical Center, Department of Medicine, Brown Medical School, 151 Research Services, 830 Chalkstone Avenue, Providence, RI 02908, USA
    Exp Cell Res 308:407-21. 2005
    ..Immunofluorescence analyses demonstrated fewer stress fibers and focal contacts in rottlerin-treated or Ad PKCdeltadn-infected EC; yet, PKCdelta inhibition caused no ..
  31. pmc A master relation defines the nonlinear viscoelasticity of single fibroblasts
    Pablo Fernandez
    Experimentalphysik I, Physikalisches Institut, Universitat Bayreuth, D 95440 Bayreuth, Germany
    Biophys J 90:3796-805. 2006
    ..We propose that the master relation reflects the mechanical behavior of the force-bearing actin cytoskeleton, in agreement with stress stiffening known from semiflexible filament networks...
  32. ncbi Oligomerization of the diaphanous-related formin FHOD1 requires a coiled-coil motif critical for its cytoskeletal and transcriptional activities
    Ricardo Madrid
    Department of Infectious Diseases, Institut Cochin, INSERM U567, CNRS UMR 8104, Universite Paris V, 27 rue du Faubourg Saint Jacques, 75014 Paris, France
    FEBS Lett 579:441-8. 2005
    ..1 (FHOD1) interacts with the Rac GTPase and activates the Rho-ROCK cascade leading to the formation of actin stress fibers. Here, we report the detection of homotypic interactions of FHOD1 in the yeast two-hybrid system, by co-..
  33. pmc Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
    Laura M Hoffman
    The Huntsman Cancer Institute and the Department of Biology, University of Utah, Salt Lake City, UT 84112, USA
    J Cell Biol 172:771-82. 2006
    ..We postulate that zyxin cooperates with Ena/VASP proteins and caldesmon to influence integrin-dependent cell motility and actin stress fiber remodeling...
  34. ncbi beta-Amyloid peptide induces formation of actin stress fibers through p38 mitogen-activated protein kinase
    Cheng Song
    Department of Pharmacology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Neurochem 83:828-36. 2002
    ..of either IAPP or mPS1 but not wild-type amyloid precursor protein or presenilin-1induced formation of actin stress fibers in SN1 cells, a septal neuronal cell line...
  35. pmc Simultaneous stretching and contraction of stress fibers in vivo
    Lynda J Peterson
    Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Biol Cell 15:3497-508. 2004
    ..We have observed that stress fibers, unlike muscle myofibrils, do not contract uniformly along their lengths...
  36. ncbi Nox1 redox signaling mediates oncogenic Ras-induced disruption of stress fibers and focal adhesions by down-regulating Rho
    Masahiro Shinohara
    Department of Molecular Biology and Biochemistry, Shinshu University Graduate School of Medicine, Matsumoto, Nagano 390 8621, Japan
    J Biol Chem 282:17640-8. 2007
    ..Nonetheless, Rho was reported to no longer couple to loss of actin stress fibers in Ras-transformed Swiss3T3 cells despite the elevation of Rho activity...
  37. pmc The role of cytoskeleton in the regulation of vascular endothelial barrier function
    Natalia V Bogatcheva
    Medical College of Georgia, Vascular Biology Center, Augusta, GA 30912, USA
    Microvasc Res 76:202-7. 2008
    ..This review will analyze the cytoskeletal elements, which reorganization affects endothelial permeability, and emphasize signaling mechanisms with barrier-protective or barrier-disruptive potential...
  38. pmc Stability of adhesion clusters and cell reorientation under lateral cyclic tension
    Dong Kong
    State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, Beijing, China
    Biophys J 95:4034-44. 2008
    ..Furthermore, we find that the stiffness and relaxation time of stress fibers play important roles in the stability of the adhesion cluster...
  39. ncbi FHOD1 is a combined actin filament capping and bundling factor that selectively associates with actin arcs and stress fibers
    André Schönichen
    Department of Physical Biochemistry, Max Planck Institute of Molecular Physiology, 44227 Dortmund, Germany
    J Cell Sci 126:1891-901. 2013
    ..At the base of lamellipodia, FHOD1 is enriched in nascent, bundled actin arcs as well as in more mature stress fibers. This function requires actin-binding domains located N-terminally to the canonical FH1-FH2 element...
  40. pmc Mechanisms of the proteinuria induced by Rho GTPases
    Liming Wang
    Division of Nephrology, Department of Medicine, Duke University and Durham VA Medical Centers, Durham, North Carolina 27710, USA
    Kidney Int 81:1075-85. 2012
    ..In contrast, the dominant-negative N19 Rho caused a loss of podocyte stress fibers, did not alter the expression of either nephrin or Rho A, and did not cause podocyte apoptosis...
  41. pmc Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions
    Kazuo Katoh
    Department of Anatomy, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke City, Tochigi 329 0498, Japan
    J R Soc Interface 8:305-11. 2011
    ..Activated Rho-kinase is also involved in the organization of newly formed stress fibres and focal adhesions in living cells...
  42. ncbi Inhibition of RhoA but not ROCK induces chondrogenesis of chick limb mesenchymal cells
    Min Jung Kim
    Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu 702 701, South Korea
    Biochem Biophys Res Commun 418:500-5. 2012
    ..CT04, induced chondrogenesis of undifferentiated mesenchymal single cells following dissolution of actin stress fibers. Inhibition of RhoA activity by CT04 was confirmed by pull down assay using the Rho-GTP binding domain of ..
  43. pmc Adenosine induces loss of actin stress fibers and inhibits contraction in hepatic stellate cells via Rho inhibition
    Muhammad A Sohail
    Section of Digestive Diseases, Yale University, New Haven, CT 06520 8019, USA
    Hepatology 49:185-94. 2009
    ..pathway is activated in differentiated hepatic stellate cells (HSCs) and is necessary for assembly of actin stress fibers, contractility, and chemotaxis...
  44. ncbi Strain waveform dependence of stress fiber reorientation in cyclically stretched osteoblastic cells: effects of viscoelastic compression of stress fibers
    Kazuaki Nagayama
    Biomechanics Laboratory, Department of Mechanical Engineering, Nagoya Institute of Technology, Nagoya, Japan
    Am J Physiol Cell Physiol 302:C1469-78. 2012
    Actin stress fibers (SFs) of cells cultured on cyclically stretched substrate tend to reorient in the direction in which a normal strain of substrate becomes zero. However, little is known about the mechanism of this reorientation...
  45. ncbi Assembly of non-contractile dorsal stress fibers requires α-actinin-1 and Rac1 in migrating and spreading cells
    Bianca Kovac
    Genome Scale Biology Program, University of Helsinki, P O Box 63, Helsinki 00014, Finland
    J Cell Sci 126:263-73. 2013
    Cell migration and spreading is driven by actin polymerization and actin stress fibers. Actin stress fibers are considered to contain α-actinin crosslinkers and nonmuscle myosin II motors...
  46. ncbi Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblasts
    K Katoh
    Department of Structural Analysis, National Cardiovascular Center Research Institute, 5 Fujishirodai, Suita, Osaka 565 8565, Japan
    Am J Physiol Cell Physiol 280:C1669-79. 2001
    ..the roles of Rho-kinase and myosin light chain kinase (MLCK) for the contraction and organization of stress fibers, we treated cultured human foreskin fibroblasts with several MLCK, Rho-kinase, or calmodulin inhibitors and ..
  47. pmc Resolving the role of actoymyosin contractility in cell microrheology
    Christopher M Hale
    Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e7054. 2009
    ..lodged in the cytoplasm, reveal the biphasic mechanical architecture of adherent cells-stiff contractile stress fibers interdigitating in a network at the cell cortex and a soft actin meshwork in the body of the cell, validate ..
  48. ncbi EspM inhibits pedestal formation by enterohaemorrhagic Escherichia coli and enteropathogenic E. coli and disrupts the architecture of a polarized epithelial monolayer
    Michal Simovitch
    Department of Microbiology and Molecular Genetics, Institute of Medical Research Israel Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel
    Cell Microbiol 12:489-505. 2010
    ..Taken together, our results show that the EspM effectors inhibit pedestal formation and induce tight junction mislocalization as well as dramatic changes in the architecture of the polarized monolayer...
  49. pmc The deficiency of PIP2 5-phosphatase in Lowe syndrome affects actin polymerization
    Sharon F Suchy
    National Human Genome Research Institute, Bethesda, MD 20892, USA
    Am J Hum Genet 71:1420-7. 2002
    ..The cellular abnormality is characterized by a decrease in long actin stress fibers, enhanced sensitivity to actin depolymerizing agents, and an increase in punctate F-actin staining in a ..
  50. pmc Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells
    Toshiyuki Watanabe
    RIKEN, Center for Developmental Biology, Kobe 650 0047, Japan
    Mol Biol Cell 18:605-16. 2007
    ..and MRLC mutants indicated that monophosphorylation of MRLC is required and sufficient for maintenance of stress fibers. Diphosphorylated MRLC stabilized myosin II filaments and was distributed locally in regions of stress fibers ..
  51. pmc A dynamic stochastic model of frequency-dependent stress fiber alignment induced by cyclic stretch
    Hui Ju Hsu
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 4:e4853. 2009
    Actin stress fibers (SFs) are mechanosensitive structural elements that respond to forces to affect cell morphology, migration, signal transduction and cell function...
  52. ncbi Cyclic stretch-induced stress fiber dynamics - dependence on strain rate, Rho-kinase and MLCK
    Chin Fu Lee
    Department of Biomedical Engineering, Texas A and M University, College Station, TX 77843 3120, United States
    Biochem Biophys Res Commun 401:344-9. 2010
    ..Instead, these distal ends were often used to assemble new stress fibers oriented progressively further away from the direction of stretch...
  53. pmc Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells
    Pirta Hotulainen
    Program in Cellular Biotechnology, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
    Mol Biol Cell 16:649-64. 2005
    ..These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility...
  54. ncbi Rho kinase inhibition initiates apoptosis in human airway epithelial cells
    Michael Moore
    University of Chicago, Section of Pulmonary and Critical Care Medicine, 5841 S Maryland Ave, MC 6076, Chicago, IL 60637, USA
    Am J Respir Cell Mol Biol 30:379-87. 2004
    ..Rho kinase, a downstream effector of RhoA, regulates the formation of stress fibers and focal adhesions. Disruption of the actin cytoskeleton causes apoptosis in airway epithelial cells...
  55. pmc Stretch-induced stress fiber remodeling and the activations of JNK and ERK depend on mechanical strain rate, but not FAK
    Hui Ju Hsu
    Department of Biomedical Engineering, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 5:e12470. 2010
    ..Cells sense and dynamically respond to stretching of the matrix by reorienting their actin stress fibers and by activating intracellular signaling proteins, including focal adhesion kinase (FAK) and the mitogen-..
  56. ncbi Actin stress fibres
    Stephanie Pellegrin
    Department of Biochemistry, School of Medical Sciences, University of Bristol, BS8 1TD, UK
    J Cell Sci 120:3491-9. 2007
    ..Some of these structures are less suited for cell motility and more suited to static contraction. In keeping with this, many specialised contractile cell types use stress fibres to remodel tissues and extracellular matrix...
  57. ncbi Tensile properties of single stress fibers isolated from cultured vascular smooth muscle cells
    Shinji Deguchi
    Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Sendai 9808579, Japan
    J Biomech 39:2603-10. 2006
    b>Stress fibers (SFs), a contractile bundle of actin filaments, play a critical role in mechanotransduction in adherent cells; yet, the mechanical properties of SFs are poorly understood...
  58. ncbi RelA/NF-kappaB transcription factor associates with alpha-actinin-4
    Vladimir N Babakov
    Department of Cell Cultures, Institute of Cytology, St Petersburg, Russian Federation
    Exp Cell Res 314:1030-8. 2008
    ..NF-kappaB and alpha-actinin-4 co-localized along actin stress fibers and in membrane lamellae in A431 cells...
  59. ncbi Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling
    Katsuhiko Asanuma
    Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Nat Cell Biol 8:485-91. 2006
    ..Together, these data show that synaptopodin is essential for the integrity of the podocyte actin cytoskeleton and for the regulation of podocyte cell migration...
  60. ncbi Actin stress fibers transmit and focus force to activate mechanosensitive channels
    Kimihide Hayakawa
    Cell Mechano sensing Project ICORP SORST, Japan Science and Technology Agency, Nagoya University Graduate School of Medicine, Nagoya, Japan
    J Cell Sci 121:496-503. 2008
    ..This study provides the first direct evidence that the cytoskeleton works as a force-transmitting and force-focusing molecular device to activate MS channels in eukaryotic cells...
  61. ncbi Cooperativity between cell contractility and adhesion
    Igor L Novak
    Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Phys Rev Lett 93:268109. 2004
    ..on experimental evidence for positive feedback between adhesion formation and assembly of actomyosin bundles (stress fibers)...
  62. ncbi DAP kinase mediates the phosphorylation of tropomyosin-1 downstream of the ERK pathway, which regulates the formation of stress fibers in response to oxidative stress
    Francois Houle
    Le Centre de Recherche en Cancérologie de l Université Laval, 9 rue McMahon, Quebec G1R 2J6, Canada
    J Cell Sci 120:3666-77. 2007
    ..the expression of the pseudophosphorylated tropomyosin mutant Ser283Glu triggers by itself the formation of stress fibers in untreated cells, and the effect is maintained in H(2)O(2)-treated cells in which DAP kinase expression is ..
  63. ncbi Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells
    Nicole Endlich
    Department of Anatomy and Cell Biology, Ernst Moritz Arndt University, D 17487 Greifswald, Germany
    Cell Motil Cytoskeleton 64:966-76. 2007
    Force generated in contractile actin filament bundles (stress fibers-SFs) is transmitted to the extracellular matrix (ECM) via linker proteins and transmembrane integrins at focal adhesions (FAs)...
  64. pmc The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells
    Lifei Fan
    Department of Biochemistry, School of Medical Sciences, University Walk, University of Bristol, Bristol BS8 1TD, UK
    J Cell Sci 123:1247-52. 2010
    Actin stress fibers are fundamental components of the actin cytoskeleton that produce contractile force in non-muscle cells...
  65. pmc Mechanical properties of actin stress fibers in living cells
    Lan Lu
    Department of Biomedical Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA
    Biophys J 95:6060-71. 2008
    Actin stress fibers (SFs) play an important role in many cellular functions, including morphological stability, adhesion, and motility...
  66. ncbi Lysophosphatidic acid down-regulates stress fibers and up-regulates pro-matrix metalloproteinase-2 activation in ovarian cancer cells
    Thuy Vy Do
    Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago Illinois, USA
    Mol Cancer Res 5:121-31. 2007
    ..Inhibition of Rho-kinase/ROCK enhanced both LPA-stimulated loss of stress fibers and pro-MMP-2 activation...
  67. ncbi The POPX2 phosphatase regulates cancer cell motility and invasiveness
    Agatha Susila
    School of Biological Sciences, Nanyang Technological University, Singapore
    Cell Cycle 9:179-87. 2010
    ..We show that silencing of POPX2 reduces the amount of stress fibers and focal adhesions in both cells lines...
  68. ncbi Functional interactions between phosphatase POPX2 and mDia modulate RhoA pathways
    Yi Xie
    School of Biological Sciences, Nanyang Technological University, Singapore, Republic of Singapore
    J Cell Sci 121:514-21. 2008
    ..We propose that the interaction between mDia1 and POPX2 (PPM1F) serves to regulate both the actin cytoskeleton and SRF-mediated transcription, and to link the CDC42/RAC1 pathways with those of RhoA...
  69. ncbi Gap junctions favor normal rat kidney epithelial cell adaptation to chronic hypertonicity
    Benedicte Desforges
    Laboratoire Structure Activité des Biomolécules Normales et Pathologiques, Institut National de la Santé et de la Recherche Médicale U, Universite Evry Val d Essonne, France
    Am J Physiol Cell Physiol 301:C705-16. 2011
    ..In addition to this, we suggest that cancer cells that do not communicate via gap junctions poorly cope with hypertonic environments thus explaining the rare occurrence of cancer coming from the kidney medulla...
  70. pmc The Rho kinases I and II regulate different aspects of myosin II activity
    Atsuko Yoneda
    Division of Biomedical Sciences, Faculty of Medicine, Imperial College London, London SW7 2AZ, England, UK
    J Cell Biol 170:443-53. 2005
    ..Specific reduction of ROCK I by siRNA resulted in loss of stress fibers and focal adhesions, despite persistent ROCK II and guanine triphosphate-bound RhoA...
  71. ncbi The tumor invasion inhibitor dihydromotuporamine C activates RHO, remodels stress fibers and focal adhesions, and stimulates sodium-proton exchange
    Lianne M McHardy
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2177 Wesbrook Mall, Vancouver, British Columbia V6T 1Z3, Canada
    Cancer Res 64:1468-74. 2004
    ..invasive MDA231 breast tumor cells that include an increase in the thickness and number of cytoplasmic actin stress fibers. Experiments with serum-starved Swiss 3T3 fibroblasts showed that micromolar concentrations of dhMotC that ..
  72. ncbi STIMulating stress fibers in endothelial cells
    Thomas Gudermann
    Walther Straub Institute of Pharmacology and Toxicology, LMU Munich, Goethestrasse 33, D 80336 Munich, Germany
    Sci Signal 6:pe8. 2013
    ..This SOCE-independent role of STIM1 highlights a surprising new aspect of this multifaceted cellular regulator...
  73. ncbi The rat homologue of Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) associates with actin filaments, recruits N-WASP from the nucleus, and mediates mobilization of actin from stress fibers in favor of filopodia formation
    Susanne Vetterkind
    Institute of Genetics, University of Bonn, Roemerstrasse 164, D 53117 Bonn, Germany
    J Biol Chem 277:87-95. 2002
    ..Expression of WIP in rat fibroblasts revealed a clear co-localization with actin stress fibers. However, expression in tumor cells lacking actin cables did not restore these structures...
  74. ncbi Dynamic reorientation of cultured cells and stress fibers under mechanical stress from periodic stretching
    K Hayakawa
    Department of Biology, Chiba University, Yayoi-cho, Chiba 263-8522, Japan
    Exp Cell Res 268:104-14. 2001
    ..Dynamic reorganization of stress fibers in living cells was visualized by labeling stress fibers with TRITC(3)-actin or EGFP-tagged moesin fragments ..
  75. ncbi Identification of FHOD1-binding proteins and mechanisms of FHOD1-regulated actin dynamics
    Jennifer J Westendorf
    The Cancer Center and Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, Minnesota, USA
    J Cell Biochem 92:29-41. 2004
    ..in vitro and a truncation mutant containing the amino-terminal 227 residues of WISH-B disrupted FHOD1-induced stress fibers. WISH-B did not affect FHOD1-induced gene transcription through the serum response factor (SRF) recognition ..
  76. pmc Mechanistic basis of Rho GTPase-induced extracellular matrix synthesis in trabecular meshwork cells
    Padmanabhan P Pattabiraman
    Dept of Ophthalmology, AERI Bldg, Rm 4010, Duke Univ School of Medicine, Durham, NC 27710, USA
    Am J Physiol Cell Physiol 298:C749-63. 2010
    ..laminin, alpha-smooth muscle actin (alpha-SMA) levels, and matrix assembly in association with increased actin stress fibers and myosin light-chain phosphorylation...
  77. ncbi p120-catenin and p190RhoGAP regulate cell-cell adhesion by coordinating antagonism between Rac and Rho
    Gregg A Wildenberg
    Department of Cancer Biology, 438 Preston Building, Vanderbilt University, Nashville, TN 37232, USA
    Cell 127:1027-39. 2006
    ..We propose that Rac activation links diverse signaling systems to AJ assembly by controlling transient p190RhoGAP interactions with p120 and localized inhibition of Rho...
  78. ncbi Phosphorylation of Caldesmon by PFTAIRE1 kinase promotes actin binding and formation of stress fibers
    Wilson K C Leung
    Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, N T, Hong Kong, China
    Mol Cell Biochem 350:201-6. 2011
    ..Without the binding of CaD to actin, disappearance of actin stress fibers was also evident in PFTK1-abrogated cells...
  79. pmc Lateral communication between stress fiber sarcomeres facilitates a local remodeling response
    Laura M Chapin
    Huntsman Cancer Institute, Departments of Biology and Oncological Sciences, Salt Lake City, UT, USA
    Biophys J 103:2082-92. 2012
    Actin stress fibers (SFs) are load-bearing and mechanosensitive structures. To our knowledge, the mechanisms that enable SFs to sense and respond to strain have not been fully defined...
  80. ncbi Intracellular stress transmission through actin stress fiber network in adherent vascular cells
    S Deguchi
    Department of Energy Systems Engineering, Graduate School of Natural Science and Technology, Okayama University, Tsushima naka 3 1 1, Okayama 7008530, Japan
    Mol Cell Biomech 2:205-16. 2005
    ..However, mechanical signaling pathway involved in intracellular force transmission through basal actin stress fibers (SFs), a mechano-responsive cytoskeletal structure, remains elusive...
  81. ncbi Theoretical concepts and models of cellular mechanosensing
    Rumi De
    Indian Institute of Science Education and Research, Kolkata, Mohanpur 741252, Nadia, West Bengal, India
    Methods Cell Biol 98:143-75. 2010
    ....
  82. pmc Dynamics of mechanical signal transmission through prestressed stress fibers
    Yongyun Hwang
    Hydrodynamics Laboratory, Department of Mechanics, Ecole Polytechnique, CNRS UMR7646, Palaiseau, France
    PLoS ONE 7:e35343. 2012
    ..We develop a model of mechanical signal transmission through prestressed viscoelastic actin stress fibers that directly connect the cell surface to the nucleus...
  83. ncbi Fibroblast growth and H-7 protein kinase inhibitor response monitored in microimpedance sensor arrays
    Duc D Nguyen
    Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Biotechnol Bioeng 87:138-44. 2004
    ..H-7 inhibits actomyosin contractility; thereafter, the loss of focal adhesion complexes occurs. The sensors, in turn, detected an impedance decrease after H-7 addition and an increase in impedance after H-7 removal...
  84. ncbi A new member of the LIM protein family binds to filamin B and localizes at stress fibers
    Toshiro Takafuta
    Department of Medicine, Cardeza Foundation for Hematologic Research, Jefferson Medical College, Philadelphia, Pennsylvania 19041, USA
    J Biol Chem 278:12175-81. 2003
    ..of FBLP-1 into 293A cells promoted stress fiber formation, and both FBLP-1 and filamin B localized to stress fibers in the transfected cells...
  85. ncbi Flow and high affinity binding affect the elastic modulus of the nucleus, cell body and the stress fibers of endothelial cells
    Anshu B Mathur
    Department of Biomedical Engineering, The University of Texas M D Anderson Cancer Center, Houston, TX, USA
    Ann Biomed Eng 35:1120-30. 2007
    ..Exposure of cells to flow increased the apparent elastic modulus of the cell body, nucleus, and stress fibers of cells adherent to the fibronectin surface...
  86. ncbi Secreted surfactant protein A from fetal membranes induces stress fibers in cultured human myometrial cells
    Michelle Breuiller-Fouche
    Institut National de la Sante et de la Recherche Medicale INSERM, U767, Faculte des Sciences Pharmaceutiques et Biologiques, Universite Paris Descartes, Paris V, Paris, France
    Am J Physiol Endocrinol Metab 298:E1188-97. 2010
    ..These data indicate that SP-A released by human fetal membranes is able to exert a paracrine regulation of F-actin filament organization in myometrial cells...
  87. ncbi Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase
    Jean Cheng Kuo
    Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan
    J Cell Sci 116:4777-90. 2003
    ..In quiescent fibroblasts, DAP kinase stabilizes stress fibers through phosphorylation of MLC, but it is dispensable for the formation of peripheral microfilament bundles...
  88. ncbi Phosphoinositide binding inhibits alpha-actinin bundling activity
    Tamara S Fraley
    Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA
    J Biol Chem 278:24039-45. 2003
    ..We conclude that the interaction of alpha-actinin with phosphoinositides regulates actin stress fibers in the cell by controlling the extent to which microfilaments are bundled.
  89. ncbi An improved confocal FRAP technique for the measurement of long-term actin dynamics in individual stress fibers
    J J Campbell
    Department of Engineering, Medical Engineering Division, Queen Mary University of London, London E1 4NS, United Kingdom
    Microsc Res Tech 70:1034-40. 2007
    ..eGFP-actin, confocal microscopy was used to bleach 3 x 1 microm regions accurately positioned along individual stress fibers. The subsequent fluorescent recovery over a 10-min imaging period was assessed from a series of intensity ..
  90. pmc Role of stress fibers and focal adhesions as a mediator for mechano-signal transduction in endothelial cells in situ
    Kazuo Katoh
    Department of Anatomy, School, of Medicine, Jichi Medical University, Yakushiji, Shimotsuke City, Tochigi, Japan
    Vasc Health Risk Manag 4:1273-82. 2008
    ..The findings of a recent study suggest that stress fibers and its associated focal adhesions play roles in mechano-signal transduction mechanism...
  91. ncbi Diphosphorylation of the myosin regulatory light chain enhances the tension acting on stress fibers in fibroblasts
    Takeomi Mizutani
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita ku, Sapporo 060 0810, Japan
    J Cell Physiol 209:726-31. 2006
    ..To show whether the diphosphorylation of MRLC increases the tension acting on stress fibers, changes in the stiffness of fibroblasts expressing wild-type MRLC and a mutant type, which cannot be ..
  92. ncbi Regulation of stretch-induced JNK activation by stress fiber orientation
    Roland Kaunas
    Whitaker Institute of Biomedical Engineering, and Department of Bioengineering, Texas A and M University, College Station, TX 77843, USA
    Cell Signal 18:1924-31. 2006
    ..uniaxial stretch, JNK activity was only transiently elevated, followed by a return to basal level as the actin stress fibers became oriented perpendicular to the direction of stretch...
  93. pmc Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling
    Naoto Kakinuma
    Signaling Molecules Research Laboratory, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8566, Japan
    J Cell Biol 181:537-49. 2008
    ..In NIH3T3 cells expressing Kank, the amount of actin stress fibers is reduced, and the coexpression of 14-3-3 disrupted this effect...
  94. ncbi Early molecular events in the assembly of the focal adhesion-stress fiber complex during fibroblast spreading
    Baruch Zimerman
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
    Cell Motil Cytoskeleton 58:143-59. 2004
    ..Upon longer incubation, they were gradually replaced by stress fibers, associated with focal adhesions at the cell periphery...
  95. ncbi Buckling of actin stress fibers: a new wrinkle in the cytoskeletal tapestry
    Kevin D Costa
    Department of Biomedical Engineering, Columbia University, New York, New York 10027, USA
    Cell Motil Cytoskeleton 52:266-74. 2002
    ..Therefore, we have developed a method to characterize the magnitude and distribution of pre-extension of actin stress fibers (SFs) due to resting tension in the cytoskeleton...
  96. ncbi Strongylophorine-26, a Rho-dependent inhibitor of tumor cell invasion that reduces actin stress fibers and induces nonpolarized lamellipodial extensions
    Lianne M McHardy
    Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3, USA
    Mol Cancer Ther 4:772-8. 2005
    ..Strongylophorine-26 induced a decrease in actin stress fibers, a dramatic increase in the size and number of focal adhesions, and the appearance of a dense meshwork of ..
  97. ncbi Epigallocatechin-3-O-gallate disrupts stress fibers and the contractile ring by reducing myosin regulatory light chain phosphorylation mediated through the target molecule 67 kDa laminin receptor
    Daisuke Umeda
    Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    Biochem Biophys Res Commun 333:628-35. 2005
    ..We show here that EGCG induced the disruption of stress fibers and decreased the phosphorylation of the myosin II regulatory light chain (MRLC) at Thr18/Ser19, which is ..
  98. pmc Rho-kinase--mediated contraction of isolated stress fibers
    K Katoh
    Department of Structural Analysis, National Cardiovascular Center Research Institute, Suita, Osaka 565 8565, Japan
    J Cell Biol 153:569-84. 2001
    ..We have recently isolated reactivatable stress fibers from cultured cells and established them as a model system for actomyosin-based contraction in nonmuscle cells...
  99. ncbi Rho-kinase dependent organization of stress fibers and focal adhesions in cultured fibroblasts
    Kazuo Katoh
    Department of Anatomy, School of Medicine, Jichi Medical University, 3311 1 Yakushiji, Shimotsuke City, Tochigi 329 0498, Japan
    Genes Cells 12:623-38. 2007
    ..is known to modulate the organization of the actin-based cytoskeletal systems, including the formation of stress fibers and focal adhesions...
  100. pmc Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling
    Zhiping Li
    Thomas Jefferson University, Department of Cancer Biology, Kimmel Cancer Center, Bluemle Building, Rm 1050, 233 South 10th St, Philadelphia, PA 19107, USA
    Mol Cell Biol 26:4240-56. 2006
    ..Cyclin D1 promotes cellular motility through inhibiting ROCK signaling and repressing the metastasis suppressor TSP-1...
  101. ncbi Phosphorylated l-caldesmon is involved in disassembly of actin stress fibers and postmitotic spreading
    Jolanta Kordowska
    Muscle and Motility Group, Boston Biomedical Research Institute, Watertown, MA 02472, USA
    Exp Cell Res 312:95-110. 2006
    ..Ser-to-Ala mutations at these two positions prevent stress fibers from disassembly upon migratory stimulation...

Research Grants63

  1. Neuropilin and semaphorin, anti-angiogenesis activities and mechanisms
    Michael Klagsbrun; Fiscal Year: 2009
    ..morphological changes in endothelial cells (EC) and tumor cells characterized by inactivation of RhoA, loss of stress fibers, collapse of the F-actin cytoskeleton and inhibition of migration...
  2. Structure and function of stress fibers and cell adhesion sites
    Mary C Beckerle; Fiscal Year: 2013
    ..Actin stress fibers undergo spontaneous cycles of thinning and repair that can be induced by the direct application of physical ..
  3. Specification of actomyosin function in the cell
    Matthew Lord; Fiscal Year: 2013
    ..Changes in actin structure facilitates cell transformation, as actomyosin stress fibers make way for a more dynamic network facilitating cell motility and metastasis...
  4. Reactive Oxygen Species Regulation of Vascular Smooth Muscle Cell Migration
    David S Weber; Fiscal Year: 2012
    ..the dephosphorylation of cofilin to regulate VSMC migration via actin depolymerization and the formation of stress fibers and/or lamellipodia, 2) test if the ROS-dependent activation of PDK1 and its role in cofilin dephosphorylation ..
  5. Macrophage Infiltration of Renal Tissue via beta-3 Integrins and Agrin
    Scott D Blystone; Fiscal Year: 2013
    ..of 23 phosphorylation results in loss of adhesion due to a failure of the actin cytoskeleton to organize into stress fibers. In this application we present evidence that this unique mechanism is required for macrophage entry into ..
  6. Cytoskeletal Pliability within Cells under Stress: RBCs and Marrow-derived SCs
    Dennis E Discher; Fiscal Year: 2012
    ..more complex marrow-derived Stem Cells, in comparison, possess a more typical cytoskeleton with a cortex and "stress fibers" that contribute to attachment as well as probing of the microenvironment in proliferation and differentiation...
  7. Human mesenchymal stem cell microvesicles for the treatment of acute lung injury
    Jae Woo Lee; Fiscal Year: 2013
    ..the increase in protein permeability in injured lung endothelial cells by preventing the formation of actin stress fibers. In Aim 3, I will determine if human MSC MV are biologically active in mice injured with E...
  8. Microtubule Stability in Lung Endothelium
    CRISTHIAAN D OCHOA ARENAS; Fiscal Year: 2012
    ..critical changes in the endothelial cell cytoskeleton, including reorganization of the cortical actin rim into stress fibers, and disassembly and reorganization of microtubules...
  9. VASCULAR ANTI-INFLAMMATORY ACTIONS OF HSP90 INHIBITORS
    John D Catravas; Fiscal Year: 2013
    ..GSK-32 and I:K1 in inflamed tissues, leading to reduced pp60c-src-dependent formation of endothelial actin stress fibers, reduced GSK-32-dependent tau phosphorylation and microtubule depolymerization, thus preventing and repairing ..
  10. Nox4 and Vascular Smooth Muscle Differentiation
    Kathy K Griendling; Fiscal Year: 2013
    ..maintenance of differentiation marker gene expression, as well as the formation of smooth muscle a-actin based stress fibers and focal adhesion turnover...
  11. Assessment of Corneal Fibroblast Biomechanical Behavior
    W Matthew Petroll; Fiscal Year: 2013
    ..which is associated with localized fibronectin secretion, cadherin expression and development of intracellular stress fibers. Using an in vivo HRT- RCM confocal microscope that we custom-modified to allow quantitative full-thickness ..
  12. NITRIC OXIDE SIGNALING MECHANISMS IN VASCULAR CELLS
    Michael Mendelsohn; Fiscal Year: 2009
    ..have an exciting phenotype supporting the above hypothesis: LZM VSMC have excessive actinomyosin stress fibers;LZM mouse blood vessels relax abnormally;and intact PKGIalpha LZM mice are hypertensive, providing exciting ..
  13. Novel Targets of Rituximab in FSGS
    SANDRA MONIKA MERSCHER; Fiscal Year: 2013
    ..Quantitative analysis of the disruption of stress fibers in vitro was associated with the degree of post-transplant proteinuria, suggesting the possibility of ..
  14. Modeling Contractile Ring Constriction in Fission Yeast
    BEN O'SHAUGHNESSY; Fiscal Year: 2013
    ..Phase A will consist in a study of stationary mammalian cell stress fibers, contractile actomyosin structures important in wound healing and other contexts...
  15. Role of Missing Metastasis Gene in tumor progression
    STEVEN ZHAN; Fiscal Year: 2013
    ..significantly altered shapes, reduced adhesiveness to extracellular matrix and the formation of actin stress fibers. Therefore, we hypothesize that MIM plays an important role in the interaction between B lineage cells and ..
  16. Genetic and Biochemical Study of V. cholerae RTX Toxin
    Karla J F Satchell; Fiscal Year: 2012
    ..We have demonstrated that this toxin depolymerizes actin stress fibers and covalently crosslinks actin into oligomers...
  17. Role of an unusual GTPase in DNA damage response and carcinogenesis
    Sam W Lee; Fiscal Year: 2012
    ..seems to be only Rho family protein transcriptionally responsive to DNA damage that can induce loss of actin stress fibers. We hypothesize that RhoE protein are essential for survival of cancer cells with wtp53 by suppressing the ..
  18. Endothelial Progenitor-derived Endothelial Cells as a Model for Familial CCM
    Christopher F Dibble; Fiscal Year: 2013
    ..loss of tube formation, invasion, increased endothelial cell monolayer permeability, and increased actin stress fibers. Significantly, these defects can be rescued by inhibition or knockdown of ROCK...
  19. Spatiotemporal Coordination of Formin and Arp2/3 in Epithelial Cell Migration
    Kwonmoo Lee; Fiscal Year: 2013
    ..filament network at the leading edge while formin is responsible for the nucleation of filopodia and stress fibers. Most existing models of cell migration entirely focus on Arp2/3-mediated actin assembly as the driver of ..
  20. Integrated System for Mechanoelectrical Studies of Cardiac Myofibroblasts
    Leslie Tung; Fiscal Year: 2012
    ..will be used to vary the shape of the myofibroblasts to manipulate their internal distribution of actin stress fibers, and to explore the effect of sub-cellular force distributions on transmembrane potential and currents...
  21. Total Synthesis of Anticancer Agent Strongylophorine-26 and Studies Directed to U
    Shengping Zheng; Fiscal Year: 2013
    ..It was observed that STP-26 reduces actin stress fibers and induces nonpolarized lamellipodial extensions...
  22. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
    ..Furthermore, PE stimulation induces the formation of stress fibers. Subsequent experiments suggested that the PE induced Erk activation can be blocked by dominant negative RhoA...
  23. THREE DIMENSIONAL STRUCTURE OF ALPHA ACTININ
    Kenneth Taylor; Fiscal Year: 2000
    ..In tissue cultured cells, alpha-actinin is located in focal adhesions and stress fibers, structural analogs of adhesion plaques and dense bodies...
  24. Role of Rho Kinase in Aqueous Humor Outflow Pathway
    P Rao; Fiscal Year: 2004
    ..of MLC phosphorylation in HTM, HSC and human ciliary muscle cells, and cytoskeletal organization (actin stress fibers, focal adhesions and adherens junctions) in HTM and HSC cells by using physiological (endothelin-1 and ..
  25. NEW BIOMEDICAL INTERFACE MATERIALS
    Roger Marchant; Fiscal Year: 2002
    ..Cell-surface interactions will be studied by analysis of actin stress fibers and focal adhesion proteins using confocal microscopy...
  26. G Proteins and Endothelial Barrier Regulation
    Tatyana Voyno Yasenetskaya; Fiscal Year: 2004
    ..In endothelial cells, thrombin activates G protein-coupled receptors and induces actin stress fibers followed by intercellular gap formation, and resultant increased trans-endothelial permeability to protein and ..
  27. CYCLIN D1 AND CELL ADHESION
    RICHARD ASSOIAN; Fiscal Year: 2007
    ..show that rho kinase-dependent stress fiber formation is also required for sustained ERK activity and that stress fibers are acting upstream to promote integrin clustering/signaling...
  28. ENDOTHELIAL CELL COMMUNICATION IN HOMEOSTASIS
    David Shepro; Fiscal Year: 1990
    ..also modulate the endothelial cytoskeleton, the current hypotheses to be examined are that: (1) cytoskeletal stress fibers are involved with endothelial structural integrity; (2) stress fiber regulation is in part a function of the ..
  29. BIOLOGY OF TRABECULAR MESHWORK IN HEALTH AND DISEASE
    BEATRICE YUE; Fiscal Year: 2009
    ..When overexpressed in TM cells, or when present extracellularly in the matrix, myocilin causes a loss of actin stress fibers and focal adhesions, and elicits a de-adhesion activity...
  30. REGULATION OF ALTERNATIVE RNA SPLICING IN A-TROPOMYOSIN
    David Wieczorek; Fiscal Year: 1992
    ..In nonmuscle cells, it is found in stress fibers, while in sarcomeric and non-sarcomeric muscle, it is a component of the thin filament...
  31. Cell Cycle Control by Rho GTPases and Their Effectors
    RICHARD ASSOIAN; Fiscal Year: 2006
    ..Second, ERK-dependent cyclin D1 expression requires the formation of stress fibers and leads to stress fiber-dependent proliferation while rac/cdc42-dependent cyclin D 1 expression and ..
  32. Phosphoinositide Regulation of Focal Adhesion Structure
    JEFFREY GREENWOOD; Fiscal Year: 2006
    ..Experiments will be carried out using slow migrating fibroblasts, which contain large focal adhesions and stress fibers and have high basal levels of Ptdlns (4,5)-Ps, and rapidly migrating U-87MG glioblastoma cells, which contain ..
  33. MOLECULAR MECHANISMS OF ACTIN DYNAMICS IN PLATELETS
    Elaine Bearer; Fiscal Year: 2003
    ..Platelets spread on glass elaborate three distinct actin structures: filopodia, lamellipodia, and stress fibers: and others have shown that three G-proteins, cdc42, rac and rho, specifically activate filopodia, ..
  34. MOLECULAR MECHANISMS OF BLOOD-BRAIN BARRIER DYSFUNCTION
    Harold Koenig; Fiscal Year: 1991
    ..and paracellular transport; (c) participation of cytoskeletal elements, including F-actin-containing stress fibers. These studies have a direct impact on the basic mechanisms underlying vasogenic brain edema and its treatment...
  35. Arg coordinates contractile forces with adhesion dynamics in migrating neurons
    JUSTIN PEACOCK; Fiscal Year: 2009
    ..to inhibit Rho-induced cell-matrix adhesions (focal adhesions) and contractile bundles of actin and myosin (stress fibers). Arg inhibition of stress fibers leads to a decrease in cell contractility...
  36. Adherens Junction Integrity and Barrier Function
    Fred Minnear; Fiscal Year: 2005
    ..Furthermore, S1P and cAMP have opposing effects on MLC phosphorylation, cellular tension, and actin stress fibers. We propose that the barrier-enhancing activity of S1P and cAMP is directed at the intercellular junctions...
  37. RhoC/GALR2 in the Metastasis of Oropharyngeal Cancers
    BRADLEY HENSON; Fiscal Year: 2007
    ..during metastatic transformation, by configuring the actin cytoskeleton for motility through the formation of stress fibers. Rho has been shown to be downstream of galanin receptor 2 (GALR2)...
  38. IN VITRO STUDIES ON CALCIUM SENSITIVE ACTIN ASSEMBLY
    Joseph Bryan; Fiscal Year: 1991
    ..antibodies (MAbs) have been prepared and show that the 90 kd protein is related to gelsolin and is located on stress fibers in WI-38 and 3T3 cells...
  39. CONTROL OF CELL ELASTICITY BY THE ACTIN CORTEX
    JOSEF KAS; Fiscal Year: 2002
    ..elastic elements: a homogeneous network of protein filaments predominantly underlying the plasma membrane and stress fibers which span the cell interior. This proposal will focus on the homogeneous cortical part of the AC...
  40. NEW BIOMEDICAL INTERFACE MATERIALS
    Roger Marchant; Fiscal Year: 2007
    ..Endothelial cell studies will include shear-dependent analysis of actin stress fibers and focal adhesion proteins using confocal microscopy...
  41. Nutrient Regulated Breast Cancer Cell De-adhesion
    Robert Hardy; Fiscal Year: 2006
    ..This state of weaker adherence is characterized by a restructuring of focal adhesion plaques and stress fibers. Extracellular regulatory factors have been proposed for de-adhesion however the role of nutritional factors ..
  42. Regulation of Pulmonary Vascular Permeability by Integrin AlphaVBeta5
    Dean Sheppard; Fiscal Year: 2009
    ..endothelial cells from increases in monolayer permeability and from both the formation of rhoA-induced actin stress fibers and the phosphorylation of components of the endothelial adherens junction...
  43. C CBL IN MODULATION OF CELL ADHESION AND MORPHOLOGY
    Alexander Tsygankov; Fiscal Year: 2005
    ..that ubiquitination-driven degradation of proteins interfering with the assembly offocal adhesions and stress fibers is involved in the effects of c-Cbl on v-Abl-transformed cells...
  44. Fogarty International Research Collaboration Award
    Keith Burridge; Fiscal Year: 2004
    ..FAs anchor stress fibers to the plasma membrane, contain multiple structural, signaling and adaptor molecules and are implicated in ..
  45. TROPOMYOSINS IN NORMAL AND TRANSFORMED CELLS
    Fumio Matsumura; Fiscal Year: 1992
    ..two actin- bundling proteins stimulate or inhibit the binding of low Mr tropomyosins to the actin bundles of stress fibers, and to determine if changes in tropomyosin-binding alter the organization of microfilaments and microtubules...
  46. PROTEINS OF BLOOD COAGULATION
    EARL DAVIE; Fiscal Year: 2006
    ..function as substrates for the GEFs and play a specific role in the development of filopodia, lamellipodia, stress fibers and focal adhesions when platelets are activated...
  47. STRUCTURAL ASSEMBLY IN MUSCLE AND NON-MUSCLE CELLS
    Yu li Wang; Fiscal Year: 1993
    ..Finally, we will study the elongation of stress fibers and myofibrils, by labeling them with fluorescent probes, and using laser microbeams to mark reference points ..
  48. Integrin Complex Nucleation of Actin
    SCOTT BLYSTONE; Fiscal Year: 2006
    ..Recent work has defined the assembly of actin into stress fibers and also the signaling underlying successful integrin-mediated adhesion...
  49. CADTK FUNCTION IN NORMAL AND NEOPLASTIC CELLS
    H Earp; Fiscal Year: 2003
    ..For example, CADTK distribution can extend beyond focal contacts to actin stress fibers. In adherent cells, CADTK is inactive until stimulated by G-protein coupled receptors or growth factors; ..
  50. CYTOSKELETAL FUNCTION IN OSTEOBLAST MECHANOTRANSDUCTION
    FREDRICK PAVALKO; Fiscal Year: 2000
    ..increased intracellular tension that occurs as a result of: 1) reorganizing actin filaments into contractile stress fibers inside bone cells, and 2) anchoring the actin filaments in stress fibers to the inner face of the plasma ..
  51. EFFECT OF HORMONES ON SIGNAL TRANSDUCTION IN BONE
    Paula Stern; Fiscal Year: 2009
    ..bone organ culture, and on cytoskeleton changes in osteoblasts including stimulation of the formation of actin stress fibers and focal adhesions, antagonism of the rapid cAMP-mediated contraction and stimulation of expression of ..
  52. STRUCTURE AND FUNCTION OF THE EXTRACELLULAR MATRIX
    A Hook; Fiscal Year: 1992
    ..g. focal adhesions and reorganize their cytoskeletal elements to form stress fibers. Formation of focal adhesions in mesenchymal cells seeded on a fibronectin substratum seems to depend on the ..
  53. Cytoskeletal Modulation of Bone Cell Mechanosensitivity
    ALEXANDER ROBLING; Fiscal Year: 2006
    ..reorganization into stress fiber bundles causes a decrease in bone cell mechanosensitivity, and disassembly of stress fibers is required to for the cell to respond to subsequent mechanical stimuli...
  54. SURFACE INFLUENCES ON CELL SIGNALING AND PROLIFERATION
    David Grainger; Fiscal Year: 2002
    ..Secondly, the applicants will investigate integrin mediated regulation of the formation of focal contacts, stress fibers and subsequent pathways for genetic regulation of cell proliferation...
  55. RhoA Signaling in Transformation by v-Src
    JOHN DONALDSON; Fiscal Year: 2004
    The long-term goal of this proposal is to understand the role of RhoA, a small GTPase that induces actin stress fibers, in v-Src transformation...
  56. Molecular Mechanisms of Regulation of Contraction
    Sarah Hitchcock DeGregori; Fiscal Year: 2005
    ..networks localized to the leading edge of the cell and not from actin filaments deeper in the cortex and in stress fibers? What protects selected populations of actin filaments from severing and pointed-end depolymerization by ADF/..
  57. Regulation of dynamic actin structures by tropomodulin
    KARI WEBER; Fiscal Year: 2006
    ..Specifically, I plan to investigate the role of tropomodulin in regulating stress fibers in endothelial cells...
  58. FUNCTION OF THE ARG PROTEIN TYROSINE KINASE
    GARY KRUH; Fiscal Year: 2001
    ..ArgBP2 is a multiple SH3 domain containing protein that is localized in stress fibers, focal adhesions and the nucleus...
  59. GAP JUNCTIONAL COMMUNICATIONS IN WOUND HEALING
    H Ehrlich; Fiscal Year: 2009
    ..abstract_text> ..
  60. Fibronectin and orientation of vascular smooth muscle
    Masaaki Yoshigi; Fiscal Year: 2006
    ..abstract_text> ..
  61. Mechanochemical regulation of endothelial permeability
    Konstantin Birukov; Fiscal Year: 2007
    ....
  62. A Request for support of 25th SPRSM Meeting
    James Wang; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  63. An interdisciplinary and integrative study of tendinopathy
    James H C Wang; Fiscal Year: 2010
    ....