Summary: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.

Top Publications

  1. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
  2. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
  3. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
  4. ncbi Rho GTPases and the actin cytoskeleton
    A Hall
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Science 279:509-14. 1998
  5. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
  6. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
  7. ncbi Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton
    D D Sarbassov
    Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Curr Biol 14:1296-302. 2004
  8. ncbi Focal adhesion kinase: in command and control of cell motility
    Satyajit K Mitra
    The Scripps Research Institute, Department of Immunology, IMM21 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 6:56-68. 2005
  9. ncbi EMT, the cytoskeleton, and cancer cell invasion
    Mahmut Yilmaz
    Institute of Biochemistry and Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland
    Cancer Metastasis Rev 28:15-33. 2009
  10. ncbi Tissue cells feel and respond to the stiffness of their substrate
    Dennis E Discher
    School of Engineering and Applied Science and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Science 310:1139-43. 2005

Detail Information

Publications352 found, 100 shown here

  1. ncbi Matrix elasticity directs stem cell lineage specification
    Adam J Engler
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, 19104, USA
    Cell 126:677-89. 2006
    ..The results have significant implications for understanding physical effects of the in vivo microenvironment and also for therapeutic uses of stem cells...
  2. pmc Lifeact: a versatile marker to visualize F-actin
    Julia Riedl
    Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nat Methods 5:605-7. 2008
    Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations...
  3. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  4. ncbi Rho GTPases and the actin cytoskeleton
    A Hall
    Medical Research Council Laboratory for Molecular Cell Biology, Cancer Research Campaign Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK
    Science 279:509-14. 1998
    The actin cytoskeleton mediates a variety of essential biological functions in all eukaryotic cells...
  5. pmc Actin, a central player in cell shape and movement
    Thomas D Pollard
    Department of Molecular, Cellular and Developmental Biology, Yale University, Post Office Box 208103, New Haven, CT 06520 8103, USA
    Science 326:1208-12. 2009
    ..Comparisons of quantitative measurements of reactions in live cells with computer simulations of mathematical models will also help generate meaningful insights...
  6. ncbi Tensional homeostasis and the malignant phenotype
    Matthew J Paszek
    Department of Bioengineering, University of Pennsylvania, Philadelphia, 19104, USA
    Cancer Cell 8:241-54. 2005
    ..Thus, ERK and Rho constitute part of an integrated mechanoregulatory circuit linking matrix stiffness to cytoskeletal tension through integrins to regulate tissue phenotype...
  7. ncbi Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton
    D D Sarbassov
    Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Curr Biol 14:1296-302. 2004
    ..a rapamycin-insensitive TOR complex that does not contain the yeast homolog of raptor and signals to the actin cytoskeleton through PKC1...
  8. ncbi Focal adhesion kinase: in command and control of cell motility
    Satyajit K Mitra
    The Scripps Research Institute, Department of Immunology, IMM21 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 6:56-68. 2005
    ..Through multifaceted and diverse molecular connections, FAK can influence the cytoskeleton, structures of cell adhesion sites and membrane protrusions to regulate cell movement.
  9. ncbi EMT, the cytoskeleton, and cancer cell invasion
    Mahmut Yilmaz
    Institute of Biochemistry and Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland
    Cancer Metastasis Rev 28:15-33. 2009
    ..processes to gain migratory and invasive properties that involve a dramatic reorganization of the actin cytoskeleton and the concomitant formation of membrane protrusions required for invasive growth...
  10. ncbi Tissue cells feel and respond to the stiffness of their substrate
    Dennis E Discher
    School of Engineering and Applied Science and Cell and Molecular Biology Graduate Group, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Science 310:1139-43. 2005
    ..Key roles in molecular pathways are played by adhesion complexes and the actinmyosin cytoskeleton, whose contractile forces are transmitted through transcellular structures...
  11. ncbi Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions
    Carsten Janke
    Department of Signalling, Neurobiology and Cancer, Institut Curie, Bat 110, Centre Universitaire, 91405 Orsay Cedex, France
    Nat Rev Mol Cell Biol 12:773-86. 2011
  12. ncbi The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:389-99. 1992
    ..These data imply that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors...
  13. ncbi Integrin signaling
    F G Giancotti
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Science 285:1028-32. 1999
    ..kinases, growth factor receptors, and ion channels and control the organization of the intracellular actin cytoskeleton. Many integrin signals converge on cell cycle regulation, directing cells to live or die, to proliferate, or to ..
  14. ncbi Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive
    Estela Jacinto
    Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
    Nat Cell Biol 6:1122-8. 2004
    ..TORC2, mTORC2 is rapamycin insensitive and seems to function upstream of Rho GTPases to regulate the actin cytoskeleton. mTORC2 is not upstream of the mTORC1 effector S6K...
  15. pmc T cell activation
    Jennifer E Smith-Garvin
    Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Annu Rev Immunol 27:591-619. 2009
    ..complex (MHC) complex pSMAC: peripheral supramolecular activation cluster PTK: protein tyrosine kinase Signal transduction: biochemical events linking surface receptor engagement to cellular responses TCR: T cell antigen receptor..
  16. ncbi The molecular motor toolbox for intracellular transport
    Ronald D Vale
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA
    Cell 112:467-80. 2003
    ..Remarkably, fungi, parasites, plants, and animals have distinct subsets of Toolbox motors in their genomes, suggesting an underlying diversity of strategies for intracellular transport...
  17. pmc Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton
    Ke Xu
    Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Methods 9:185-8. 2012
    ..we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-..
  18. ncbi Endothelial cell migration during angiogenesis
    Laurent Lamalice
    Le Centre de recherche en cancérologie, l Université Laval, L Hôtel Dieu de Québec, Quebec, Canada
    Circ Res 100:782-94. 2007
    ..The aim of this review is to give an integrative view of the signaling mechanisms that govern endothelial cell migration in the context of angiogenesis...
  19. ncbi Adhesion signaling - crosstalk between integrins, Src and Rho
    Stephan Huveneers
    Division of Toxicology, Leiden Amsterdam Center for Drug Research, Leiden University, 2300 RA Leiden, The Netherlands
    J Cell Sci 122:1059-69. 2009
    ..Integrins sense the physical properties of the extracellular matrix and organize the cytoskeleton accordingly...
  20. pmc Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges
    Daniel A Starr
    Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Annu Rev Cell Dev Biol 26:421-44. 2010
    The nuclear envelope links the cytoskeleton to structural components of the nucleus. It functions to coordinate nuclear migration and anchorage, organize chromatin, and aid meiotic chromosome pairing...
  21. ncbi Septins: the fourth component of the cytoskeleton
    Serge Mostowy
    Institut Pasteur, Unité des Interactions Bactéries Cellules, Département de Biologie Cellulaire et Infection, F 75015 Paris, France
    Nat Rev Mol Cell Biol 13:183-94. 2012
    ..of proteins that is highly conserved in eukaryotes and is increasingly recognized as a novel component of the cytoskeleton. All septins are GTP-binding proteins that form hetero-oligomeric complexes and higher-order structures, ..
  22. ncbi Integrins: bidirectional, allosteric signaling machines
    Richard O Hynes
    Howard Hughes Medical Institute, Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 110:673-87. 2002
    ..between their extracellular ligand binding adhesion sites and their cytoplasmic domains, which link to the cytoskeleton and to signal transduction pathways...
  23. pmc Cell detachment activates the Hippo pathway via cytoskeleton reorganization to induce anoikis
    Bin Zhao
    Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang, China
    Genes Dev 26:54-68. 2012
    ..However, little is known about the signals that regulate the Hippo pathway. Here we report that through cytoskeleton reorganization, cell detachment activates the Hippo pathway kinases Lats1/2 and leads to YAP phosphorylation ..
  24. pmc Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity
    Mutsuki Amano
    Department of Cell Pharmacology, Nagoya University, Showa Ku, Japan
    Cytoskeleton (Hoboken) 67:545-54. 2010
    ..This review focuses on the structure, function, and modes of activation and action of Rho-kinase...
  25. pmc Vinculin controls focal adhesion formation by direct interactions with talin and actin
    Jonathan D Humphries
    Wellcome Trust Centre for Cell Matrix Research, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 179:1043-57. 2007
    ..The vinculin tail, however, is responsible for the functional link of FAs to the actin cytoskeleton. We propose a new model that explains how vinculin orchestrates FAs.
  26. ncbi Rho GTPases and cell migration
    A J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, UK
    J Cell Sci 114:2713-22. 2001
    Cell migration involves dynamic and spatially regulated changes to the cytoskeleton and cell adhesion. The Rho GTPases play key roles in coordinating the cellular responses required for cell migration...
  27. pmc Functional atlas of the integrin adhesome
    Ronen Zaidel-Bar
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, 76100, Israel
    Nat Cell Biol 9:858-67. 2007
    ..We discuss the role of the different network modules in regulating the structural and signalling functions of cell-matrix adhesions...
  28. pmc Native ultrastructure of the red cell cytoskeleton by cryo-electron tomography
    Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, New York, USA
    Biophys J 101:2341-50. 2011
    Erythrocytes possess a spectrin-based cytoskeleton that provides elasticity and mechanical stability necessary to survive the shear forces within the microvasculature...
  29. ncbi Tubulin post-translational modifications: encoding functions on the neuronal microtubule cytoskeleton
    Carsten Janke
    Institut Curie, Batiment 110, Centre Universitaire, F 91405 Orsay cedex, France
    Trends Neurosci 33:362-72. 2010
    ..of these modifications and provided first insights into their potential functions in regulating the microtubule cytoskeleton. By contrast, several tubulin-modifying enzymes were only discovered in the last few years, and studies on ..
  30. ncbi The matrix corroded: podosomes and invadopodia in extracellular matrix degradation
    Stefan Linder
    Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Ludwig Maximilians Universitat, Pettenkoferstr 9, 80336 Munchen, Germany
    Trends Cell Biol 17:107-17. 2007
    ..This review highlights important new developments in the field, discusses the common and divergent features of podosomes and invadopodia and summarizes current knowledge about matrix-degrading proteinases at these structures...
  31. ncbi Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines
    P Mundel
    Department of Anatomy and Cell Biology, University of Heidelberg, Germany
    Exp Cell Res 236:248-58. 1997
    ..Therefore, the determinative steps of podocyte differentiation and process formation are studied for the first time using an inducible in vitro model...
  32. ncbi Biology of the p21-activated kinases
    Gary M Bokoch
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Annu Rev Biochem 72:743-81. 2003
    ..We propose here a key role for PAK action in coordinating the dynamics of the actin and microtubule cytoskeletons during directional motility of cells, as well as in other functions requiring cytoskeletal polarization...
  33. ncbi Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
    Tony Yeung
    Institute for Medicine and Engineering, University of Pennsylvania, PA 19104, USA
    Cell Motil Cytoskeleton 60:24-34. 2005
    ..These results support the hypothesis that mechanical factors impact different cell types in fundamentally different ways, and can trigger specific changes similar to those stimulated by soluble ligands...
  34. pmc Subversion of the actin cytoskeleton during viral infection
    Matthew P Taylor
    Department of Molecular Biology, Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey 08544, USA
    Nat Rev Microbiol 9:427-39. 2011
    ..describe how the interaction of viral proteins with the cell modulates the structure and function of the actin cytoskeleton to initiate, sustain and spread infections...
  35. ncbi The LIM domain: from the cytoskeleton to the nucleus
    Julie L Kadrmas
    Huntsman Cancer Institute and the Department of Biology, University of Utah, 2000 East, Circle of Hope, Salt Lake City, Utah 84112, USA
    Nat Rev Mol Cell Biol 5:920-31. 2004
    ..An emerging theme is that LIM proteins might function as biosensors that mediate communication between the cytosolic and the nuclear compartments...
  36. ncbi The cytoskeleton and cancer
    Alan Hall
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Cancer Metastasis Rev 28:5-14. 2009
    ..These are all aspects of cell behavior than often go awry in cancer. This volume brings together those interested in understanding the contribution of the actin and microtubule cytoskeletons to the cell biology of cancer...
  37. pmc The evolution of the cytoskeleton
    Bill Wickstead
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, England, UK
    J Cell Biol 194:513-25. 2011
    The cytoskeleton is a system of intracellular filaments crucial for cell shape, division, and function in all three domains of life...
  38. ncbi The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
    A J Ridley
    Institute for Cancer Research, Chester Beatty Laboratories, London, England
    Cell 70:401-10. 1992
    ..We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin...
  39. pmc Integration of contractile forces during tissue invagination
    Adam C Martin
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, NJ 08544, USA
    J Cell Biol 188:735-49. 2010
    Contractile forces generated by the actomyosin cytoskeleton within individual cells collectively generate tissue-level force during epithelial morphogenesis...
  40. ncbi Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding
    Martin P Stewart
    ETH Zurich, Department of Biosystems Science and Engineering, CH 4058 Basel, Switzerland
    Nature 469:226-30. 2011
    ..We find that this mitotic rounding force depends both on the actomyosin cytoskeleton and the cells' ability to regulate osmolarity. The rounding force itself is generated by an osmotic pressure...
  41. ncbi The SUN rises on meiotic chromosome dynamics
    Yasushi Hiraoka
    Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita 565 0871, Japan
    Dev Cell 17:598-605. 2009
    ..During meiotic prophase, the apparent role of these SUN-domain proteins, together with their partner KASH-domain proteins, is to connect chromosomes through the intact nuclear envelope to force-generating mechanisms in the cytoplasm...
  42. ncbi Cytoskeletal regulation of pulmonary vascular permeability
    S M Dudek
    Division of Pulmonary and Critical Care Medicine, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA
    J Appl Physiol 91:1487-500. 2001
    ..Both competing forces in this model are intimately linked through the endothelial cytoskeleton, a complex network of actin microfilaments, microtubules, and intermediate filaments, which combine to regulate ..
  43. ncbi Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides
    Juha Saarikangas
    Program in Cell and Molecular Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland
    Physiol Rev 90:259-89. 2010
    The plasma membrane and the underlying cortical actin cytoskeleton undergo continuous dynamic interplay that is responsible for many essential aspects of cell physiology...
  44. ncbi Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins
    Toshiki Itoh
    Department of Cell Biology and Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Dev Cell 9:791-804. 2005
    ..We report here that a family of proteins previously implicated in the regulation of the actin cytoskeleton also have powerful lipid bilayer-deforming properties via an N-terminal module (F-BAR) similar to the BAR ..
  45. pmc Establishment of HIV-1 latency in resting CD4+ T cells depends on chemokine-induced changes in the actin cytoskeleton
    Paul U Cameron
    Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria 3004, Australia
    Proc Natl Acad Sci U S A 107:16934-9. 2010
    ..We propose a unique pathway for establishment of latency by direct HIV-1 infection of resting CD4(+) T cells during normal chemokine-directed recirculation of CD4(+) T cells between blood and tissue...
  46. ncbi Neuronal polarization: the cytoskeleton leads the way
    Michael Stiess
    Axonal Growth and Regeneration Group, Max Planck Institute of Neurobiology, Am Klopferspitz 18, Martinsried, Germany
    Dev Neurobiol 71:430-44. 2011
    ..This process of neuronal polarization is driven by the cytoskeleton. The first and decisive event during neuronal polarization is the specification of the axon...
  47. ncbi Differential gene regulation under altered gravity conditions in follicular thyroid cancer cells: relationship between the extracellular matrix and the cytoskeleton
    Claudia Ulbrich
    Institute of Clinical Pharmacology and Toxicology, Charite Universitatsmedizin Berlin, Berlin, Germany
    Cell Physiol Biochem 28:185-98. 2011
    ..After 22 s microgravity, the F-actin and cytokeratin cytoskeleton was altered, and ACTB and KRT80 mRNAs were significantly upregulated after the first and thirty-first parabolas...
  48. pmc Bringing KASH under the SUN: the many faces of nucleo-cytoskeletal connections
    David Razafsky
    Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA
    J Cell Biol 186:461-72. 2009
    ..However, the recent identification of evolutionarily conserved linkers of the nucleoskeleton to the cytoskeleton (LINC) complexes, a family of macromolecular assemblies that span the double membrane of the nuclear envelope, ..
  49. pmc Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness
    P J Stewart-Hutchinson
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Exp Cell Res 314:1892-905. 2008
    ..within the perinuclear space establish physical connections, called LINC complexes, between the nucleus and the cytoskeleton. Here, we show that the KASH domains of Nesprins 1, 2 and 3 interact promiscuously with luminal domains of Sun1 ..
  50. ncbi Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
    N Yang
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Nature 393:809-12. 1998
    ..Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin...
  51. ncbi Cell-matrix adhesion
    Allison L Berrier
    Katrina Visiting Faculty Program, National Center on Minority Health and Health Disparities, National Institutes of Health, Bethesda, Maryland, USA
    J Cell Physiol 213:565-73. 2007
    ..The review concludes by speculating on the application of this knowledge of cell-matrix interactions in the formation of cell adhesions, assembly of matrix, migration, and tumorigenesis to potential future therapeutic approaches...
  52. ncbi A tale of two Paks
    Luis E Arias-Romero
    Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, U S A
    Biol Cell 100:97-108. 2008
    ..In this review, we discuss recent advances in our understanding of the structure, regulation and function of these kinases, emphasizing the many differences between these two groups of signalling proteins...
  53. pmc Phosphorylation of ezrin/radixin/moesin proteins by LRRK2 promotes the rearrangement of actin cytoskeleton in neuronal morphogenesis
    Loukia Parisiadou
    Unit of Transgenesis, Laboratory of Neurogenetics, National Institute on Aging, NIH, Bethesda, MD 20892, USA
    J Neurosci 29:13971-80. 2009
    ..These data support a model in which the G2019S mutation of LRRK2 causes a gain-of-function effect that perturbs the homeostasis of pERM and F-actin in sprouting neurites critical for neuronal morphogenesis...
  54. pmc Adherens and tight junctions: structure, function and connections to the actin cytoskeleton
    Andrea Hartsock
    Departments of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305 5430, USA
    Biochim Biophys Acta 1778:660-9. 2008
    ..Both junctional complexes are proposed to associate with the actin cytoskeleton, and formation and maturation of cell-cell contacts involves reorganization of the actin cytoskeleton...
  55. pmc Mechanical signaling through the cytoskeleton regulates cell proliferation by coordinated focal adhesion and Rho GTPase signaling
    Paolo P Provenzano
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Sci 124:1195-205. 2011
    ..mediated by Rho GTPases, to provide a functional linkage through which forces are transmitted through the cytoskeleton by intracellular force-generating proteins...
  56. ncbi Sticky business: orchestrating cellular signals at adherens junctions
    Mirna Perez-Moreno
    Laboratory of Mammalian Cell Biology and Development, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 112:535-48. 2003
    ..Deciphering the underlying mechanisms that govern these conserved cellular rearrangements is a prerequisite to understanding vertebrate morphogenesis...
  57. ncbi Cadherin junctions and their cytoskeleton(s)
    William M Brieher
    Department of Cell and Developmental Biology, University of Illinois, Urbana, IL 61801, USA
    Curr Opin Cell Biol 25:39-46. 2013
    Classical cadherin adhesion receptors exert many of their biological effects through close cooperation with the cytoskeleton. Much attention has focused on attempting to understand the physical interactions between cadherin molecular ..
  58. ncbi Tumor invasion in the absence of epithelial-mesenchymal transition: podoplanin-mediated remodeling of the actin cytoskeleton
    Andreas Wicki
    Institute of Biochemistry and Genetics, Department of Clinical Biological Sciences, Center of Biomedicine, University of Basel, 4058 Basel, Switzerland
    Cancer Cell 9:261-72. 2006
    ..In conclusion, podoplanin induces an alternative pathway of tumor cell invasion in the absence of epithelial-mesenchymal transition (EMT)...
  59. pmc The relationship between force and focal complex development
    Catherine G Galbraith
    Duke University Medical Center, Durham, NC 27710, USA
    J Cell Biol 159:695-705. 2002
  60. ncbi Regulation of cancer cell motility through actin reorganization
    Daisuke Yamazaki
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, Minato ku, Japan
    Cancer Sci 96:379-86. 2005
    ..The different types of cell migration are regulated by different mechanisms. Reorganization of the actin cytoskeleton is the primary mechanism of cell motility and is essential for most types of cell migration...
  61. ncbi HDAC6: a key regulator of cytoskeleton, cell migration and cell-cell interactions
    Agustin Valenzuela-Fernandez
    Departamento de Medicina Física y Farmacología, Facultad de Medicina and Instituto de Tecnologías Biomédicas ITB, Universidad de La Laguna, Tenerife, Spain
    Trends Cell Biol 18:291-7. 2008
    ..examines the biological actions of HDAC6, focusing on its deacetylase activity and its potential scaffold functions in the regulation of cell migration and other key biological processes in which the cytoskeleton plays an important role.
  62. ncbi mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes
    Beate Vollenbröker
    UKM, Medizinische Klinik und Poliklinik D, Abteilung Molekulare Nephrologie, Domagkstr 3A, D 48149 Munster, Germany
    Am J Physiol Renal Physiol 296:F418-26. 2009
    ..Our data provide new molecular insights explaining which pathways and molecules are affected in podocytes by an imbalanced mTOR function because of rapamycin treatment...
  63. ncbi The mechanical integrin cycle
    Eileen Puklin-Faucher
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Cell Sci 122:179-86. 2009
    ..mechanotransduction is the cycle by which integrins bind to the matrix at the leading cell edge, attach to the cytoskeleton, transduce mechanical force, aggregate in the plasma membrane as part of increasingly strengthened adhesion ..
  64. ncbi Actin cytoskeleton regulation in neuronal morphogenesis and structural plasticity
    Liqun Luo
    Department of Biological Sciences, Neurosciences Program, Stanford University, California 94305, USA
    Annu Rev Cell Dev Biol 18:601-35. 2002
    The actin cytoskeleton plays a major role in morphological development of neurons and in structural changes of adult neurons...
  65. pmc Cell mechanics and the cytoskeleton
    Daniel A Fletcher
    Bioengineering and Biophysics, University of California, Berkeley, California 94720, USA
    Nature 463:485-92. 2010
    ..cell to resist deformation, to transport intracellular cargo and to change shape during movement depends on the cytoskeleton, an interconnected network of filamentous polymers and regulatory proteins...
  66. pmc A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii
    Brooke R Anderson-White
    Department of Biology, Boston College, Chestnut Hill, MA, USA
    Cell Microbiol 13:18-31. 2011
    ..The cytoskeleton of Toxoplasma, which is composed of microtubules associated with an inner membrane complex (IMC), has an ..
  67. ncbi Viral interactions with the cytoskeleton: a hitchhiker's guide to the cell
    Kerstin Radtke
    Institute of Virology, OE5230, Hannover Medical School, Carl Neuberg Str 1, D 30625 Hannover, Germany
    Cell Microbiol 8:387-400. 2006
    The actin and microtubule cytoskeleton play important roles in the life cycle of every virus...
  68. pmc Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling
    Julian C Boggiano
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Dev Cell 22:695-702. 2012
    ..In this review, we discuss recent findings that emphasize the cell cortex-specifically the actin cytoskeleton, intercellular junctions, and protein complexes that determine cell polarity-as a key site for Hippo pathway ..
  69. pmc Organisation and regulation of the cytoskeleton in plant programmed cell death
    A Smertenko
    School of Biological and Biomedical Sciences, Durham University, Durham, DH1 3LE, UK
    Cell Death Differ 18:1263-70. 2011
    ..Signalling to PCD results in major reorganisation of cellular components. The plant cytoskeleton is known to play a major role in cellular organisation, and reorganization and alterations in its dynamics is a ..
  70. ncbi Nesprins LINC the nucleus and cytoskeleton
    Jason A Mellad
    BHF Centre of Research Excellence, Division of Cardiovascular Medicine, King s College London, SE5 9NU, UK
    Curr Opin Cell Biol 23:47-54. 2011
    ..proteins, nesprins co-ordinate and maintain cellular architecture by linking membranous organelles to the cytoskeleton. However nuclear envelope (NE) nesprins, uniquely hardwire the nuclear lamina to the cytoskeleton and molecular ..
  71. ncbi Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking
    Anne J Ridley
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, 91 Riding House Street, London W1W 7BS, UK
    Trends Cell Biol 16:522-9. 2006
  72. ncbi Adhesion-dependent cell mechanosensitivity
    Alexander D Bershadsky
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot 76100, Israel
    Annu Rev Cell Dev Biol 19:677-95. 2003
    ..At these sites, transmembrane integrin receptors are associated via their cytoplasmic domains with the actin cytoskeleton. This interaction with actin is mediated by a submembrane plaque, consisting of numerous cytoskeletal and ..
  73. pmc FtsZ in bacterial cytokinesis: cytoskeleton and force generator all in one
    Harold P Erickson
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    Microbiol Mol Biol Rev 74:504-28. 2010
    ..Recent efforts to develop antibacterial drugs that target FtsZ are reviewed. Finally, we discuss evidence of how FtsZ generates a constriction force: by protofilament bending into a curved conformation...
  74. ncbi Small GTP-binding proteins
    Y Takai
    Department of Molecular Biology, Osaka University Graduate School of Medicine Faculty of Medicine, Suita, Japan
    Physiol Rev 81:153-208. 2001
    ..Cascades and cross-talks of small G proteins have also been clarified. In this review, functions of small G proteins and their modes of activation and action are described...
  75. ncbi Actin dynamics at the leading edge: from simple machinery to complex networks
    Robert H Insall
    CRUK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland
    Dev Cell 17:310-22. 2009
    ..Migration requires the actin cytoskeleton, specifically the localized polymerization of actin filaments underneath the plasma membrane...
  76. ncbi IQGAP1 in cellular signaling: bridging the GAP
    Matthew D Brown
    Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Trends Cell Biol 16:242-9. 2006
    ..It modulates the actin cytoskeleton through Rac1 and Cdc42, and cell-cell adhesion through E-cadherin and beta-catenin...
  77. pmc How HIV takes advantage of the cytoskeleton in entry and replication
    Bettina Stolp
    Department of Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany
    Viruses 3:293-311. 2011
    The host cell cytoskeleton plays a key role in the life cycle of viral pathogens whose propagation depends on mandatory intracellular steps...
  78. pmc Hts/Adducin controls synaptic elaboration and elimination
    Jan Pielage
    Department of Biochemistry and Biophysics, University of California, San Francisco, 1550 4 th Street, San Francisco, CA 94158, USA
    Neuron 69:1114-31. 2011
    ..Hts/Adducin may define a mechanism to switch between synapse stability and dynamics...
  79. ncbi Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis
    S Kothakota
    Chiron Corporation, Emeryville, CA 94608, USA
    Science 278:294-8. 1997
    ..Thus, cleaved gelsolin may be one physiological effector of morphologic change during apoptosis...
  80. pmc The interaction between nesprins and sun proteins at the nuclear envelope is critical for force transmission between the nucleus and cytoskeleton
    Maria L Lombardi
    Department of Medicine, Brigham and Women s Hospital Harvard Medical School, Cambridge, Massachusetts 02139, USA
    J Biol Chem 286:26743-53. 2011
    Maintaining physical connections between the nucleus and the cytoskeleton is important for many cellular processes that require coordinated movement and positioning of the nucleus...
  81. ncbi Podocyte biology and response to injury
    Peter Mundel
    Division of Nephrology, Albert Einstein College of Medicine, Bronx, New York, USA
    J Am Soc Nephrol 13:3005-15. 2002
  82. ncbi Crosstalk between small GTPases and polarity proteins in cell polarization
    Sandra Iden
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Nat Rev Mol Cell Biol 9:846-59. 2008
    ..Recent findings provide insights into polarization mechanisms and show intriguing crosstalk between small GTPases and members of polarity complexes in regulating cell polarization in different cellular contexts and cell types...
  83. ncbi Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1
    David A Guertin
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Department of Biology, Cambridge, Massachusetts 02141, USA
    Dev Cell 11:859-71. 2006
    ..Thus, mTORC1 function is essential in early development, mLST8 is required only for mTORC2 signaling, and mTORC2 is a necessary component of the Akt-FOXO and PKCalpha pathways...
  84. pmc Two components of actin-based retrograde flow in sea urchin coelomocytes
    J H Henson
    Department of Biology, Dickinson College, Carlisle, Pennsylvania 17013, USA
    Mol Biol Cell 10:4075-90. 1999
    ..Inhibition of actin polymerization with cytochalasin D caused the actin cytoskeleton to separate from the cell margin and undergo a finite retrograde retraction...
  85. pmc Dysfunctional connections between the nucleus and the actin and microtubule networks in laminopathic models
    Christopher M Hale
    Department of Chemical and Biomolecular Engineering, Washington University School of Medicine, St Louis, Missouri, USA
    Biophys J 95:5462-75. 2008
    ..Recent work has identified nuclear envelope proteins--emerin and the linker of the nucleoskeleton and cytoskeleton (LINC) complex--which connect the nuclear lamina to the cytoskeleton...
  86. pmc Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence
    Jochen Guck
    Institute for Soft Matter Physics, Department of Physics and Geosciences, University of Leipzig, 04103 Leipzig, Germany
    Biophys J 88:3689-98. 2005
    ..The cytoskeleton, an internal polymer network, in particular determines a cell's mechanical strength and morphology...
  87. ncbi Mechanotransduction - a field pulling together?
    Christopher S Chen
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Cell Sci 121:3285-92. 2008
    ..through external forces that are applied to tissues or endogenous forces that are generated within the active cytoskeleton. Despite the wide array of studies demonstrating that such forces affect cellular signaling and function, it ..
  88. pmc Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins
    Zhanxiang Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Cell Sci 122:893-903. 2009
    ..Nearly 40 years ago, the actin cytoskeleton was first recognized as a key mediator of biphasic insulin release, and was originally presumed to act as a ..
  89. pmc Structure of Sad1-UNC84 homology (SUN) domain defines features of molecular bridge in nuclear envelope
    Zhaocai Zhou
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 287:5317-26. 2012
    ..The LINC (linker of nucleoskeleton and cytoskeleton) complex, formed by the SUN and the nesprin proteins at the nuclear envelope, serves as a mechanical linkage ..
  90. ncbi Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, London, United Kingdom
    J Cell Physiol 176:150-65. 1998
    ..Our data suggest that Cdc42, Rac, and Rho are activated in a hierarchical cascade following stimulation with TNF-alpha leading to actomyosin-mediated cell retraction and formation of intercellular gaps...
  91. pmc Deconstructing the cadherin-catenin-actin complex
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 123:889-901. 2005
    ..extracellular domains of the cadherin family of adhesion proteins, and strengthened by linkage to the actin cytoskeleton. Prevailing dogma is that cadherins are linked to the actin cytoskeleton through beta-catenin and alpha-catenin,..
  92. pmc The Wiskott-Aldrich syndrome: The actin cytoskeleton and immune cell function
    Michael P Blundell
    Molecular Immunology Unit, UCL Institute of Child Health, London, UK
    Dis Markers 29:157-75. 2010
    ..WASP is key in transducing signals from the cell surface to the actin cytoskeleton, and a lack of WASp results in cytoskeletal defects that compromise multiple aspects of normal cellular ..
  93. ncbi Paxillin and focal adhesion signalling
    C E Turner
    Department of Cell and Developmental Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Cell Biol 2:E231-6. 2000
    ..Paxillin is a multi-domain adaptor found at the interface between the plasma membrane and the actin cytoskeleton. Here it provides a platform for the integration and processing of adhesion- and growth factor-related signals.
  94. ncbi TGN golgins, Rabs and cytoskeleton: regulating the Golgi trafficking highways
    Bruno Goud
    Institut Curie, CNRS UMR144, 26, rue d Ulm, Paris cedex 75248, France
    Trends Cell Biol 20:329-36. 2010
    ..We explore the potential for TGN golgins, acting as scaffold molecules, to co-ordinate the regulation of TGN structure and function...
  95. ncbi Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
    Benjamin D Matthews
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 119:508-18. 2006
    ..Thus, cells use multiple mechanisms to sense and respond to static and dynamic changes in the level of mechanical stress applied to integrins...
  96. pmc Direct dynamin-actin interactions regulate the actin cytoskeleton
    Changkyu Gu
    Nephrology Division, Department of Medicine, Harvard Medical School and Massachusetts General Hospital, Charlestown, MA 02129, USA
    EMBO J 29:3593-606. 2010
    ..Dynamin also regulates the actin cytoskeleton through an unknown, GTPase-dependent mechanism...
  97. ncbi alpha-Catenin as a tension transducer that induces adherens junction development
    Shigenobu Yonemura
    Electron Microscope Laboratory, RIKEN Center for Developmental Biology, Kobe, Hyogo 650 0047, Japan
    Nat Cell Biol 12:533-42. 2010
    Adherens junctions (AJs), which are organized by adhesion proteins and the underlying actin cytoskeleton, probably sense pulling forces from adjacent cells and modulate opposing forces to maintain tissue integrity, but the regulatory ..
  98. ncbi Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus
    Joseph M Miano
    Cardiovascular Research Institute, University of Rochester School of Medicine, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Am J Physiol Cell Physiol 292:C70-81. 2007
    ..and growth, these genetic studies point to a crucial role for SRF in cellular migration and normal actin cytoskeleton and contractile biology...
  99. ncbi IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions
    Giorgio Scita
    IFOM FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy School of Medicine, University of Milan, 20122 Milan, Italy
    Trends Cell Biol 18:52-60. 2008
    ..Structural, biochemical and cell biological experiments support the unique role of this family as transducers of signalling, linking the protruding membrane to the underlying actin cytoskeleton.
  100. ncbi Insights into mesenchymal stem cell aging: involvement of antioxidant defense and actin cytoskeleton
    Grit Kasper
    Julius Wolff Institute and Center for Musculoskeletal Surgery Berlin, Charite Universitatsmedizin Berlin, Berlin, Germany
    Stem Cells 27:1288-97. 2009
    ..Functional annotation clustering revealed that age-affected molecular functions are associated with cytoskeleton organization and antioxidant defense...
  101. ncbi An insider's guide to the microtubule cytoskeleton of Giardia
    Scott C Dawson
    Department of Microbiology, UC Davis, CA 95616, USA
    Cell Microbiol 12:588-98. 2010
    Giardia intestinalis is a zoonotic, parasitic protist with a complex microtubule cytoskeleton critical for motility, attachment, intracellular transport, cell division and transitioning between its two life cycle stages - the cyst and the ..

Research Grants72

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..Cytoskeletal elements, including actin-based corrals and cortical cytoskeleton networks, restrict receptor mobility and may also serve as tethers for protein islands...
  3. Gamma-Synuclein-Mediated Regulation of Norepinephrine Transporter
    Anita Sidhu; Fiscal Year: 2010
    ..the function and trafficking of the NE transporter [NET], through interactions with the microtubule [MT] cytoskeleton. We show here that -Syn can also regulate these NET activities. Such modulation by -Syn is unique to NET...
  4. Regulation of B Cell Function by Membrane-Cytoskeletal Remodeling
    Neetu Gupta; Fiscal Year: 2012
    ..Membrane remodeling requires membrane uncoupling from the underlying cortical cytoskeleton but its regulation and impact on B cell function is poorly understood...
  5. Investigating Mechanism of Intracellular Rotational Transport with Optical T
    Jeffrey N Anker; Fiscal Year: 2012
    ..for uptake, degradation and clearance of foreign particles, all biophysical processes which rely upon an intact cytoskeleton. Impairment of these biophysical processes due to nanoparticle-mediated cytoskeletal oxidation contributes to ..
  6. Role of JAK2-PAK1 interaction in prolactin-dependent signaling
    Maria Diakonova; Fiscal Year: 2013
    ..protein-protein interactions, leading to the formation of a multiprotein complex that modulates the actin cytoskeleton, increases cell motility and invasiveness, mediates cyclin D1 gene transcription and affects tumorigenicity of ..
  7. Axonopathy in Glaucoma
    Samuel D Crish; Fiscal Year: 2013
    ..Specific aim 1 will examine changes in the cytoskeleton compared to two major risk factors in glaucoma: age (DBA/2J mice) and exposure to elevated intraocular pressure ..
  8. Regulation of AKAP79 Postsynaptic Membrane Targeting
    MARK L DELL'ACQUA; Fiscal Year: 2012
    ..synapses, AMPA (AMPAR) and NMDA (NMDAR) glutamate receptors are linked to signaling proteins and the actin cytoskeleton in dendritic spines through a network of scaffolding proteins that play important roles during synaptic ..
  9. Cellular Signaling in Alcoholism
    Subhash C Pandey; Fiscal Year: 2013
    ..Third, the effector immediate-early gene Arc (activity- regulated cytoskeleton associated protein) may be relevant in regulating dendritic spine density (DSD) because it is rapidly induced ..
    Mary K Sheats; Fiscal Year: 2013
    ..neutrophils coordinate chemoattractant signals in their external environment with reorganization of their actin cytoskeleton. The Myristolated Alanine-Rich C-Kinase Substrate (MARCKS) family of proteins, MARCKS and MARCKS-like 1 (..
    Francis W Luscinskas; Fiscal Year: 2013
    ..both of which involve coordinated interactions of cell-cell adhesion/junctional molecules and the actin cytoskeleton. Dysregulation of these endothelial functions promotes inflammatory disease processes...
    Alex Brown; Fiscal Year: 2010
    ..Phospholipase D generates phosphatidic acid from phosphatidylcholine, to regulate organization of the actin cytoskeleton, cell growth, survival, motility and vesicular transport...
  13. Identification of Proteins Important for Male Osteoporosis
    Hong Wen Deng; Fiscal Year: 2013
    ..problem is subcellular proteome extraction to stepwise isolate proteins from membrane, cytosol, nucleus, and cytoskeleton E fractions followed by sensitive 2D-nanoLC-ESI-MS/MS for fractioning and identifying significant proteins...
  14. Interplay Between Tissue Architecture and Nuclear Organization in the DNA Damage
    Pierre Alexandre Vidi; Fiscal Year: 2013
    ..hypothesis will be tested with engineered hydrogels of defined stiffness, interference with the cytoskeleton dynamics, and uncoupling integrins from the cytoskeleton...
  15. 4th ASM Conference on Prokaryotic Cell Biology and Development
    Susan S Golden; Fiscal Year: 2012
    ..insights in our understanding of essential cell structures including the cell division apparatus, the cytoskeleton, the cell wall, and structures involved in bacterial motility...
  16. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..of Foxc2 localized to the cytoplasm will determine upregulation of Foxc2 on [unreadable]-actinin and cytoskeleton rearrangements...
  17. Signaling Mechanisms in Alcohol Drinking Behaviors
    Subhash C Pandey; Fiscal Year: 2013
    ..Our laboratory recently demonstrated that brain-derived neurotrophic factor (BDNF), activity-regulated cytoskeleton-associated protein (Arc) expression, and dendritic spine density (DSD) were lower in the central (CeA) and ..
  18. Growth Hormone Regulation of SH2B1
    Christin Carter-Su; Fiscal Year: 2013
    ..SH2B1 is recruited to JAK2 and is a critical component in GH regulation of the actin cytoskeleton, a prerequisite for cellular proliferation, differentiation and migration...
  19. RSK-2 regulates integrin-mediated adhesion and migration
    JOE WILLIAM RAMOS; Fiscal Year: 2013
    ..RSK2 modulates integrin function downstream of ERK by regulating integrin 2 tail interaction with the actin cytoskeleton. The research plan has three specific aims: (1) Establish the molecular mechanism by which RSK2 modulates ..
  20. Osteoclastic Protein-Tyrosine Phosphatase and Resorption
    Kin Hing William Lau; Fiscal Year: 2013
    ..over-expression of EphA4 in wild-type precursors on the formation, fusion, adhesion, migration, actin cytoskeleton re-organization, and resorption activity of osteoclasts in vitro...
    Quentin J Baca; Fiscal Year: 2010
    ..There is biochemical evidence that populations of CD45 reside in lipid microdomains and associate with the cytoskeleton, and it is proposed that these interactions regulate CD45 function...
  22. Neuropilin and semaphorin, anti-angiogenesis activities and mechanisms
    Michael Klagsbrun; Fiscal Year: 2009
    ..EC) and tumor cells characterized by inactivation of RhoA, loss of stress fibers, collapse of the F-actin cytoskeleton and inhibition of migration...
    Bruce S McEwen; Fiscal Year: 2013
    ..activate genes related to oxidative stress and possibly inflammation and also alter neurotransmission and cytoskeleton. We plan to identify genes and gene products related to stress and GC effects by perturbing the endocannabinoid ..
  24. The Regulation of Salivary Gland Regeneration by P2Y2 Nucleotide Receptors
    GARY ANDREW WEISMAN; Fiscal Year: 2013
    ..Specific Aim 3 will determine whether P2Y2R interaction with the actin cytoskeleton regulates tight junction organization in Par-C10 monolayers...
  25. Identifying underlying mechanisms of intracellular changes in response to caregiv
    MELANIE SARAH FLINT; Fiscal Year: 2013
    ..T-cell migration is a complex, not yet well understood process;however it is known that a functional cytoskeleton and actin polymerization is essential...
  26. Fic-mediated Adenylylation
    CAROLYN ANN WORBY; Fiscal Year: 2013
    ..Contrary to our expectations, we observed that the YopT-like domain of IbpA does not disrupt the actin cytoskeleton despite its conservation of the key catalytic C/H/D triad...
  27. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..complex fulfills in a key role in remodeling the actin filament network, a major component of the cellular cytoskeleton. The complex is essential for changes to cell shape associated with specific processes, such as endocytosis in ..
  28. Adhesion of Leptospira interrogans to Human Cell Surface Receptors
    Jenifer L Coburn; Fiscal Year: 2010
    ..endothelial cell monolayers, as revealed by microarray analyses of transcript levels, staining of the actin cytoskeleton, and rounding of the cells...
  29. In vivo analysis of the mechanisms of axon transport.
    Catherine M Drerup; Fiscal Year: 2011
    ..kinase (Jnk) activity in cell culture, which could potentially have downstream effects on the microtubule cytoskeleton and cargo-motor binding...
    Ralph A Nixon; Fiscal Year: 2013
    ..Defining the specific steps in this regulation will help to clarify mechanisms leading to major neurological diseases, including dementias, and will identify novel cellular targets for developing innovative therapies for these diseases. ..
  31. Rho-Modifying Cytotoxic Necrotizing Factor of E. coli
    ALISON DAVIS O'BRIEN; Fiscal Year: 2013
    ..These deamidation events trigger a myriad of effects on the target cells such as actin cytoskeleton rearrangements, cell cycle abnormalities, and alterations in signaling pathways...
  32. Identification of Mechanoresponsive Promoter Elements in Chondrogenesis
    Dominik R Haudenschild; Fiscal Year: 2013
    ..These approaches have successfully identi- fied integrins, focal adhesion complexes, the cytoskeleton, and select kinase pathways as me- diators of mechanotransduction...
  33. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..multivariable roles in modifying host or bacterial proteins involved in lipid and membrane transport, the actin cytoskeleton, microtubule-based motors, lysosomal recognition of the inclusion, ERK/MEK signaling pathways, and the onset of ..
    JAMES CARLTON GARRISON; Fiscal Year: 2010
    ..mice, and mTORC2 is a newly discovered rapamycin-insensitive signaling complex that controls both the actin cytoskeleton and phosphorylation of Akt...
  35. Antigen receptor inputs: linking structural, molecular, and cellular responses
    Jonathan P Schneck; Fiscal Year: 2012 affect T cell receptor clustering -glycosylation and the role of cholesterol and its interaction with the cytoskeleton. Second we will analyze the impact of genes identified in the screen in binding of a TCR ligand, soluble ..
    Philip B Wedegaertner; Fiscal Year: 2013 dynamic cell architecture changes brought about by Rho's role in modifying a cell's actin cytoskeleton. Cellular processes under the control of Rho GTPases include smooth muscle cell contractility, cell adhesion, ..
  37. Structure and function of stress fibers and cell adhesion sites
    Mary C Beckerle; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The actin cytoskeleton is essential for cell viability and plays critical roles in cell shape, adhesion, and motility...
  38. Role of Integrins in Bladder Urothelium
    Warren G Hill; Fiscal Year: 2013
    ..broad family of cell surface adhesion and signaling molecules which link the extracellular matrix (ECM) to the cytoskeleton of the cell...
  39. Functional analysis of mDia formins in hematopoietic stem cell engraftment and mi
    Peng Ji; Fiscal Year: 2010
    ..signaling pathways regulating HSC engraftment and migration often directly or indirectly target on the actin cytoskeleton network through proteins that regulate polymerization of the actin proteins...
  40. Regulation of ENaC in salt-sensitive hypertension via inflammation-induced ROS pr
    ..It is further hypothesized that increased H2O2 production results in changes of the actin cytoskeleton and that cortactin and MIM proteins are involved in this mechanism...
    Christopher E Turner; Fiscal Year: 2012
    ..control of cell adhesion to the extracellular matrix (ECM) in coordination with remodeling of the actin cytoskeleton. The Rho family GTPases play a central role in this regulation but the mechanisms controlling the activity of ..
    Daniel R Schoenberg; Fiscal Year: 2013
    ..Consistent with this, PMR1 binds to the Ena/VASP proteins, which are regulators of the actin cytoskeleton, and cell motility is increased in cells ex- pressing catalytically-active PMR1...
  43. Role of the Tail Domain in Vinculin Function
    Sharon L Campbell; Fiscal Year: 2010
    ..Vinculin functions as a mechanical linker between transmembrane integrin receptors and the actin cytoskeleton through association with cell adhesion molecules...
  44. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..C. elegans studies of the regulation of the actin cytoskeleton by the Extracellular Matrix during embryonic development in the Soto Lab are identifying new roles for axonal ..
  45. Dysfunction of neurofilaments in disease: an iPS model of Giant Axonal Neuropathy
    ..neurons and should inform our understanding the pathogenesis of GAN and other neurodegenerative diseases of the cytoskeleton. PUBLIC HEALTH RELEVANCE: In the autosomal recessive disease Giant Axonal Neuropathy (GAN), degeneration of ..
  46. Cytoskeletal Keratins in Epithelial Immunity to Bacterial Keratitis
    K P CONNIE TAM; Fiscal Year: 2013
    ..which are glycine-rich C-terminal fragments of keratin 6A, a major intermediate filament protein found in the cytoskeleton of a wide range of epithelial cells...
  47. Mechanics and regulation of chromosome dynamics in meiotic prophase
    MICHAEL EDWARD DRESSER; Fiscal Year: 2012
    ..cerevisiae, telomeres connect across the nuclear envelope to elements of the actin cytoskeleton that drag the chromosomes along the nuclear periphery at rates up to ~2 5m/second...
    EunDuck P Kay; Fiscal Year: 2010
    ..We also showed that FGF-2 reorganizes actin cytoskeleton using Rho kinases (active Rac and Cdc42, and inactive Rho), thus generating a migratory cell phenotype...
  49. Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin Dynamics
    Fabeha Fazal; Fiscal Year: 2013
    ..We previously showed that activation of RhoA/ROCK and the dynamic changes in actin cytoskeleton induced by thrombin are crucial for NF-(B activation and ICAM-1 expression...
  50. Role of AIE in Epigenetic and Behavioral Changes at Adulthood
    Subhash C Pandey; Fiscal Year: 2013
    ..Several genes, such as brain-derived neurotrophic factor (BDNF), activity regulated cytoskeleton-associated (Arc) protein, and NMDA receptor subunits (NR2A&2B) are involved in regulating dendritic spine ..
  51. Encapsidation of the poxvirus genome
    Paula Traktman; Fiscal Year: 2012
    ..As a result, the progression of the viral life cycle is intimately linked to interactions with the organelles, cytoskeleton and cytoplasmic milieu of the host cell...
    MARK A MC NIVEN; Fiscal Year: 2013
    ..We have found that this contractile polymer associates with the actin cytoskeleton and specific adaptor proteins at both the PM to regulate receptor endocytosis and the TGN to mediate ..
  53. Signaling of Integrin Alpha 7
    Jianhua Luo; Fiscal Year: 2009
    ..we also found that ILK, a serine/threonine kinase and structural protein associated with integrins and the cytoskeleton, bound and phosphorylated a tumor suppressor gene called myopodin, which regulates cell growth and cell ..
  54. Release of FGF1 and Pathology of Angiogenesis
    Igor Prudovsky; Fiscal Year: 2010
    ..studies on liposomes and cell membrane vesicles, expression of specific regulators of the submembrane actin cytoskeleton will be used in Specific Aims 1 and 2...
  55. Role of Cofilin and Wnt Signaling in Neural Crest Development
    O apos NEIL WIGGAN; Fiscal Year: 2013
    Project Summary/Abstract Abstract: A key aspect of cell and tissue morphogenesis is regulation of the actin cytoskeleton. The actin cytoskeleton is a fundamental cellular scaffold which dictates cell morphology and multiple important ..
  56. Growth Hormone Regulation of SH2-B1
    Christin Carter Su; Fiscal Year: 2009
    ..Independent of its ability to activate JAK2, SH2B1 enhances GH regulation of the actin cytoskeleton and cell motility. Finally, SH2B1 shuttles between the plasma membrane, cytoplasm and nucleus...
    Nigel W Bunnett; Fiscal Year: 2010
    ..The role of the cytoskeleton and of endosomal acidification and phosphatases will be examined using specific drugs Aim 2 will define the ..
  58. Signaling of Integrin Alpha 7
    Jianhua Luo; Fiscal Year: 2013
    ..we also found that ILK, a serine/threonine kinase and structural protein associated with integrins and the cytoskeleton, bound and phosphorlyated a tumor suppressor gene called myopodin, which regulates cell growth and cell ..
  59. Role of trabecular meshwork contractility in modulating outflow resistance
    CHEE HIAN TAN; Fiscal Year: 2013
    ..His specific cell biological focus was in cytoskeleton and cell- extracellular matrix interactions, with correlation to functional changes in live monkey hydrodynamic ..
  60. Rapid ischemic tolerance: Synaptic re-organization and reduced excitotoxicity
    Robert Meller; Fiscal Year: 2012 and synaptic reorganization leading to b) the disruption of NMDA receptor anchoring to the cytoskeleton, c) thus altering NMDA receptor function with resultant protection from harmful ischemia...
  61. Epigenetic regulation of viral infection and replication by periodontal bacteria
    Fengchun Ye; Fiscal Year: 2013
    ..Previous studies suggest that lysine acetylation of cytoskeleton actins and microtubule plays important roles in viral entry and intracellular trafficking...
  62. Exploration of the functions of the ciliopathy Arls in cilia.
    Jinghua Hu; Fiscal Year: 2013
    ..Small GTPases act as key molecular switches in diverse membrane- and cytoskeleton-related cellular processes. However, the roles of Arl family members are poorly defined...
  63. Modulation of Monocarboxylic Acid Transporter Kinetic Function for Stroke Therapy
    JEFFREY PAUL SMITH; Fiscal Year: 2010
    ..This regulatory action is dependent upon dynamic changes in the Actin cytoskeleton which facilitates trafficking and serves as an anchor for the transducisome...
  64. Pathogen-host Interactions that Remodel the Cytoskeleton
    Tina Izard; Fiscal Year: 2010
    The deadly enteropathogen Shigella flexneri usurps components of the actin cytoskeleton for entry and movement in the host cell, and for the formation of protrusions used to infect neighboring cells...
    David Bredt; Fiscal Year: 2002
    Disruptions of the myofiber cytoskeleton underlie several genetic muscular dystrophies, including Duchenne and Limb-girdle muscular dystrophies...
  66. Matrix-induced Myogenesis & Pharmaco-Screens of MSCs
    Dennis Discher; Fiscal Year: 2009
    ..interacts with chromatin and through linker proteins that span the envelope the lamina also interacts with the cytoskeleton. We have shown recently that the cytoskeleton is strongly influenced by matrix elasticity in acto-myosin ..
  67. Investigating the molecular and mechanical regulation of pulsed actomyosin contra
    ADAM CHRISTOPHER MARTIN; Fiscal Year: 2010
    ..Specifically, I gained much experience working with the actin cytoskeleton, which generates mechanical forces in cells...
  68. Molecular Function of Palladin's Ig Domains in Cell Adhesion and Motility
    Carol A Otey; Fiscal Year: 2010
    ..provided by applicant): Both cell-cell and cell-matrix adhesions require the participation of the actin cytoskeleton. Actin filaments are crosslinked into circumferential rings that are associated with cell-cell junctions and ..
    James Russell; Fiscal Year: 2001
    ..results in downstream phosphorylation of focal adhesion proteins involved in organization of the actin cytoskeleton and neurite formative, and 3) IGF-IR activation of phosphatidylinositol-3 kinase (PI-3K) is essential for ..
  70. Cytoskeletal organization in apicomplexan parasites
    Heide Schatten; Fiscal Year: 2005
    ..Despite the importance of the cytoskeleton for cell division in these unicellular organisms the mechanisms that power the separation of the genome remain ..
    Richard Andersen; Fiscal Year: 1991
    ..The most clearly demonstrated "on-off" mechanism occurs in the red blood cell (RBC), where the cytoskeleton is attached to transmembrane receptors...
  72. Colloquium on the Cytoskeleton and Human Disease
    Leslie Wilson; Fiscal Year: 2001
    A broadly focused international research meeting on the cytoskeleton and its associated proteins in human diseases is planned from April 17 through April 20, 2001...