cytoplasm

Summary

Summary: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)

Top Publications

  1. pmc Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6
    Caroline Vance
    Department of Clinical Neuroscience, King s College London, Medical Research Council MRC Centre for Neurodegeneration Research, Institute of Psychiatry, London SE5 8AF, UK
    Science 323:1208-11. 2009
  2. ncbi Messenger-RNA-binding proteins and the messages they carry
    Gideon Dreyfuss
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Nat Rev Mol Cell Biol 3:195-205. 2002
  3. pmc Mutant FUS proteins that cause amyotrophic lateral sclerosis incorporate into stress granules
    Daryl A Bosco
    Department of Neurology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Hum Mol Genet 19:4160-75. 2010
  4. ncbi HuR and mRNA stability
    C M Brennan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Cell Mol Life Sci 58:266-77. 2001
  5. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
  6. ncbi Integrins: bidirectional, allosteric signaling machines
    Richard O Hynes
    Howard Hughes Medical Institute, Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 110:673-87. 2002
  7. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
  8. pmc Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae
    Carolyn J Decker
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721
    J Cell Biol 179:437-49. 2007
  9. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
  10. ncbi The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress
    Yoshiharu Kawaguchi
    Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
    Cell 115:727-38. 2003

Research Grants

  1. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
  2. Enhanced iron removal with peptide linked chelators targeted to ferritin
    Elizabeth C Theil; Fiscal Year: 2010
  3. GENETIC ANALYSIS OF POLARITY
    RUTH M STEWARD; Fiscal Year: 2010
  4. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
  5. Scott J Hultgren; Fiscal Year: 2015
  6. Cellular and Animal Models of FUS Mutations in ALS
    Eric J Huang; Fiscal Year: 2013
  7. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
  8. Preclinical drug trial in mouse models of inflammation
    Kanneboyina Nagaraju; Fiscal Year: 2011
  9. Intracellular Serpin Regulation of Intestinal Cell Necrosis
    GARY ARTHUR SILVERMAN; Fiscal Year: 2012
  10. Christin Carter-Su; Fiscal Year: 2016

Detail Information

Publications541 found, 100 shown here

  1. pmc Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6
    Caroline Vance
    Department of Clinical Neuroscience, King s College London, Medical Research Council MRC Centre for Neurodegeneration Research, Institute of Psychiatry, London SE5 8AF, UK
    Science 323:1208-11. 2009
    ..FUS is involved in the regulation of transcription and RNA splicing and transport, and it has functional homology to another ALS gene, TARDBP, which suggests that a common mechanism may underlie motor neuron degeneration...
  2. ncbi Messenger-RNA-binding proteins and the messages they carry
    Gideon Dreyfuss
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    Nat Rev Mol Cell Biol 3:195-205. 2002
    From sites of transcription in the nucleus to the outreaches of the cytoplasm, messenger RNAs are associated with RNA-binding proteins...
  3. pmc Mutant FUS proteins that cause amyotrophic lateral sclerosis incorporate into stress granules
    Daryl A Bosco
    Department of Neurology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Hum Mol Genet 19:4160-75. 2010
    ..These findings demonstrate a potential link between FUS mutations and cellular pathways involved in stress responses that may be relevant to altered motor neuron homeostasis in ALS...
  4. ncbi HuR and mRNA stability
    C M Brennan
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Cell Mol Life Sci 58:266-77. 2001
    ..The influence of newly identified protein ligands to HuR on HuR function in both normal and stressed cells may explain how ARE-mediated mRNA decay is regulated in response to environmental change...
  5. pmc Decapping and decay of messenger RNA occur in cytoplasmic processing bodies
    Ujwal Sheth
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA
    Science 300:805-8. 2003
    ..These results define the flux of mRNAs between polysomes and P bodies as a critical aspect of cytoplasmic mRNA metabolism and a possible site for regulation of mRNA degradation...
  6. ncbi Integrins: bidirectional, allosteric signaling machines
    Richard O Hynes
    Howard Hughes Medical Institute, Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 110:673-87. 2002
    ..Long-range conformational changes couple these functions via allosteric equilibria...
  7. pmc Visualization of the intracellular behavior of HIV in living cells
    David McDonald
    Dept of Microbiology and Immunology, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612, USA
    J Cell Biol 159:441-52. 2002
    To track the behavior of human immunodeficiency virus (HIV)-1 in the cytoplasm of infected cells, we have tagged virions by incorporation of HIV Vpr fused to the GFP...
  8. pmc Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae
    Carolyn J Decker
    Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721
    J Cell Biol 179:437-49. 2007
    ..These results suggest a stepwise model for P-body assembly with the initial formation of a core mRNA-protein complex that then aggregates through multiple specific mechanisms...
  9. ncbi The Wnt signaling pathway in development and disease
    Catriona Y Logan
    Department of Developmental Biology, Beckman Center, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
    Annu Rev Cell Dev Biol 20:781-810. 2004
    ..The next few years are likely to see novel therapeutic reagents aimed at controlling Wnt signaling in order to alleviate these conditions...
  10. ncbi The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress
    Yoshiharu Kawaguchi
    Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA
    Cell 115:727-38. 2003
    ..Misfolded protein aggregates are transported and removed from the cytoplasm by dynein motors via the microtubule network to a novel organelle termed the aggresome where they are processed...
  11. pmc The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
    T Kirisako
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, Japan
    J Cell Biol 151:263-76. 2000
    Autophagy and the Cvt pathway are examples of nonclassical vesicular transport from the cytoplasm to the vacuole via double-membrane vesicles...
  12. ncbi Duration of nuclear NF-kappaB action regulated by reversible acetylation
    - Chen Lf
    Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94141, USA
    Science 293:1653-7. 2001
    ..of the IkappaB inhibitors, which normally bind and sequester this pleiotropically active factor in the cytoplasm. The subsequent molecular events that regulate the termination of nuclear NF-kappaB action remain poorly defined,..
  13. ncbi RIG-I-mediated antiviral responses to single-stranded RNA bearing 5'-phosphates
    Andreas Pichlmair
    Immunobiology Laboratory, Cancer Research UK, London Research Institute, London WC2A 3PX, UK
    Science 314:997-1001. 2006
    ....
  14. pmc A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus
    L D Mayo
    Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Proc Natl Acad Sci U S A 98:11598-603. 2001
    ..Phosphorylation on these sites is necessary for translocation of Mdm2 from the cytoplasm into the nucleus...
  15. pmc An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm
    Miles R Armstrong
    Plant Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, UK
    Proc Natl Acad Sci U S A 102:7766-71. 2005
    ..We used association genetics to identify Avr3a and show that it encodes a protein that is recognized in the host cytoplasm, where it triggers R3a-dependent cell death. Avr3a resides in a region of the P...
  16. ncbi Oscillations in NF-kappaB signaling control the dynamics of gene expression
    D E Nelson
    Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool, L69 7ZB, UK
    Science 306:704-8. 2004
    ..The functional consequences of NF-kappaB signaling may thus depend on number, period, and amplitude of oscillations...
  17. pmc ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS
    Angela Alexander
    Department of Carcinogenesis, University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA
    Proc Natl Acad Sci U S A 107:4153-8. 2010
    ..in response to elevated ROS, ATM activates the TSC2 tumor suppressor via the LKB1/AMPK metabolic pathway in the cytoplasm to repress mTORC1 and induce autophagy...
  18. ncbi Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function
    Venkat S R K Yedavalli
    Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, Bethesda, MD 20892, USA
    Cell 119:381-92. 2004
    ..Plausibly, DDX3 is the human RNA helicase which functions in the CRM1 RNA export pathway analogously to the postulated role for Dbp5p in yeast mRNA export...
  19. ncbi Nuclear export of microRNA precursors
    Elsebet Lund
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
    Science 303:95-8. 2004
    ..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
  20. pmc TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm
    Alberto De Iaco
    Department of Microbiology and Molecular Medicine, University of Geneva, 1205, Geneva, Switzerland
    Retrovirology 10:20. 2013
    ..Yet, an equal number of investigators have failed to obtain evidence that supports this model. Here, a series of experiments were performed to better elucidate the mechanism by which TNPO3 promotes HIV-1 infectivity...
  21. ncbi Exportin 1 (Crm1p) is an essential nuclear export factor
    K Stade
    Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0414, USA
    Cell 90:1041-50. 1997
    ..cerevisiae. The presented data are consistent with the conclusion that Crm1p is a carrier for the NES-mediated protein export pathway. We propose CRM1 be renamed exportin 1 (XPO1)...
  22. ncbi Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transduction
    Gary Davidson
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Nature 438:867-72. 2005
    ..Our results reveal an evolutionarily conserved mechanism that couples Wnt receptor activation to the cytoplasmic signal transduction apparatus...
  23. doi Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
    ..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
  24. pmc Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing
    Zhonghua Wang
    Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Province, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    Plant Cell 18:676-87. 2006
    ..Here, we show in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene ..
  25. ncbi Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation
    Josipa Bilic
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Science 316:1619-22. 2007
    ..We propose that Wnts induce coclustering of receptors and Dvl in LRP6-signalosomes, which in turn triggers LRP6 phosphorylation to promote Axin recruitment and beta-catenin stabilization...
  26. ncbi Mono- versus polyubiquitination: differential control of p53 fate by Mdm2
    Muyang Li
    Institute for Cancer Genetics and Department of Pathology, College of Physicians and Surgeons, Columbia University, 1150 St Nicholas Avenue, New York, NY 10032, USA
    Science 302:1972-5. 2003
    ..A p53-ubiquitin fusion protein that mimics monoubiquitinated p53 was found to accumulate in the cytoplasm in an Mdm2-independent manner, indicating that monoubiquitination is critical for p53 trafficking...
  27. ncbi Measurement of dynamic protein binding to chromatin in vivo, using photobleaching microscopy
    Robert D Phair
    BioInformatics Services, Rockville, Maryland 20854, USA
    Methods Enzymol 375:393-414. 2004
    ..These methods should prove useful in the further in vivo investigation of the molecular mechanisms involved in genome organization and expression...
  28. doi Mutations in the FUS/TLS gene on chromosome 16 cause familial amyotrophic lateral sclerosis
    T J Kwiatkowski
    Department of Neurology, Massachusetts General Hospital, 114 16th Street, Charlestown, MA 02129, USA
    Science 323:1205-8. 2009
    ..In contrast, the mutant forms of FUS/TLS accumulated in the cytoplasm of neurons, a pathology that is similar to that of the gene TAR DNA-binding protein 43 (TDP43), whose mutations ..
  29. pmc Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion
    Teresa L M Thurston
    MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Hills Road, Cambridge CB2 0QH, UK
    Nature 482:414-8. 2012
    ..Our results illustrate how cells deploy the danger receptor galectin 8 to combat infection by monitoring endosomal and lysosomal integrity on the basis of the specific lack of complex carbohydrates in the cytosol...
  30. ncbi Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA
    B Wolff
    Sandoz Research Institute, Brunner Strasse 59, A1230 Vienna, Austria
    Chem Biol 4:139-47. 1997
    ..1 (HIV-1) regulatory protein Rev is required for unspliced and incompletely spliced viral mRNAs to appear in the cytoplasm and thus for viral replication...
  31. pmc Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo
    Christel Rouget
    mRNA Regulation and Development, Institute of Human Genetics, CNRS UPR1142, 141 rue de la Cardonille, Cedex 5, 34396 Montpellier, France
    Nature 467:1128-32. 2010
    ..Because the piRNAs involved in this regulation are produced from transposable elements, this identifies a direct developmental function for transposable elements in the regulation of gene expression...
  32. ncbi Macromolecular crowding: an important but neglected aspect of the intracellular environment
    R J Ellis
    Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    Curr Opin Struct Biol 11:114-9. 2001
    ..It is proposed that the addition of crowding agents should become as routine as controlling pH and ionic strength if we are to meet the objective of studying biological molecules under more physiologically relevant conditions...
  33. ncbi Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells
    B P Zhou
    Department of Molecular and Cellular Oncology, Breast Cancer Basic Research Program, University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 3:245-52. 2001
    ..Our results indicate that HER-2/neu induces cytoplasmic localization of p21Cip1/WAF1 through activation of Akt to promote cell growth, which may have implications for the oncogenic activity of HER-2/neu and Akt...
  34. pmc DJ-1 is a redox-dependent molecular chaperone that inhibits alpha-synuclein aggregate formation
    Shoshana Shendelman
    Department of Pathology, Center for Neurobiology and Behavior, and Taub Institute, Columbia University, College of Physicians and Surgeons, New York, New York, USA
    PLoS Biol 2:e362. 2004
    ..We further demonstrate that DJ-1 chaperone activity in vivo extends to alpha-synuclein, a protein implicated in PD pathogenesis...
  35. doi Quantitative analysis of low-abundance peptides in HeLa cell cytoplasm by targeted liquid chromatography/mass spectrometry and stable isotope dilution: emphasising the distinction between peptide detection and peptide identification
    Thierry Le Bihan
    Centre for Systems Biology at Edinburgh, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK
    Rapid Commun Mass Spectrom 24:1093-104. 2010
    ..An in silico analysis of the human trypsinosome allows the evaluation of how unique are the sets of features that we are using for peptide detection...
  36. pmc The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins
    Eric W Humke
    Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA
    Genes Dev 24:670-82. 2010
    ..In the absence of signaling, Sufu restrains Gli3 in the cytoplasm, promoting its processing into a repressor. Initiation of signaling triggers the dissociation of Sufu from Gli3...
  37. pmc Protein folding in the cytoplasm and the heat shock response
    R Martin Vabulas
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cold Spring Harb Perspect Biol 2:a004390. 2010
    ....
  38. pmc Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA)
    Ruonan Zhang
    Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America
    PLoS ONE 5:e15620. 2010
    ..via RT step and the preformed viral proteins are assembled into pre-integration complex (PIC) in the cell cytoplasm. To integrate the viral DNA into the host genome, the PIC must cross cell nuclear membrane through the nuclear ..
  39. pmc Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts
    Anuja Guria
    Institut fur Biochemie, OE4310, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    RNA 17:1048-56. 2011
    ..We further identified 10 genes that are spliced but not exported to the cytoplasm in the absence of THOC5. These mRNAs were copurified with THOC5...
  40. doi Tuned Escherichia coli as a host for the expression of disulfide-rich proteins
    Gustavo Salinas
    Catedra de Inmunologia, Facultad de Quimica Facultad de Ciencias, Instituto de Higiene, Universidad de la Republica, Montevideo, Uruguay
    Biotechnol J 6:686-99. 2011
    ..coli...
  41. pmc Hierarchical regulation of mRNA partitioning between the cytoplasm and the endoplasmic reticulum of mammalian cells
    Qiang Chen
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 22:2646-58. 2011
    ..These data indicate that mRNAs are partitioned between the cytosol and ER compartments via a hierarchical system of intrinsic and encoded topogenic signals and identify mRNA cohort-restricted modes of mRNA association with the ER...
  42. pmc Poly(ADP-ribose) regulates post-transcriptional gene regulation in the cytoplasm
    Anthony Leung
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA
    RNA Biol 9:542-8. 2012
    ..We recently described two novel functions for pADPr in the cytoplasm, both of which involve RNA metabolism - 1) the assembly of cytoplasmic stress granules, cellular macrostructures ..
  43. pmc Cargo proteins facilitate the formation of transport vesicles in the cytoplasm to vacuole targeting pathway
    Takahiro Shintani
    Life Sciences Institute and Departments of Molecular, Cellular, and Developmental Biology and Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 279:29889-94. 2004
    ..The cytoplasm to vacuole targeting (Cvt) pathway is an unconventional vesicular trafficking pathway in yeast, which is ..
  44. pmc The exosome contains domains with specific endoribonuclease, exoribonuclease and cytoplasmic mRNA decay activities
    Daneen Schaeffer
    Department of Microbiology and Molecular Genetics, University of Texas Health Science Center Houston, 6431 Fannin Street, MSB 1 212 Houston, Texas 77030, USA
    Nat Struct Mol Biol 16:56-62. 2009
    ..The combination of an endoRNase and an exoRNase activity seems to be a widespread feature of RNA-degrading machines...
  45. pmc Localization of Chlamydia trachomatis hypothetical protein CT311 in host cell cytoplasm
    Lei Lei
    Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    Microb Pathog 51:101-9. 2011
    ..The extra-inclusion CT311 molecules were distributed in the host cell cytoplasm with a pattern similar to that of CPAF, a known Chlamydia-secreted protease...
  46. ncbi Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype
    Brunangelo Falini
    Institute of Hematology, University of Perugia, Perugia, Italy
    N Engl J Med 352:254-66. 2005
    ..Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein...
  47. ncbi Identification of genes required for cytoplasmic localization in early C. elegans embryos
    K J Kemphues
    Section of Genetics and Development Cornell University Ithaca, New York 14853
    Cell 52:311-20. 1988
    ..We propose that all of these defects result from the failure of a maternally encoded system for intracellular localization in early embryos...
  48. ncbi The nucleoporin RanBP2 has SUMO1 E3 ligase activity
    Andrea Pichler
    Max Planck Institute for Biochemistry, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Cell 108:109-20. 2002
    ..Our findings place SUMOylation at the cytoplasmic filaments of the NPC and suggest that, at least for some substrates, modification and nuclear import are linked events...
  49. pmc Control of poly(A) polymerase level is essential to cytoplasmic polyadenylation and early development in Drosophila
    François Juge
    Génétique du Développement de la Drosophile, Institut de Genetique Humaine, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    EMBO J 21:6603-13. 2002
    ..This demonstrates that regulation of the PAP level is essential for controlled cytoplasmic polyadenylation and early development...
  50. ncbi The molecular architecture of the nuclear pore complex
    Frank Alber
    Department of Bioengineering and Therapeutic Sciences, and California Institute for Quantitative Biosciences, Mission Bay QB3, 1700 4th Street, Suite 503B, University of California at San Francisco, San Francisco, California 94158 2330, USA
    Nature 450:695-701. 2007
    ..These findings provide clues to the evolutionary origins of the NPC...
  51. doi Crowding effects on diffusion in solutions and cells
    James A Dix
    Department of Chemistry, State University of New York, Binghamton, New York 13902, USA
    Annu Rev Biophys 37:247-63. 2008
    ..We conclude that the notion of universally anomalous diffusion in cells as a consequence of molecular crowding is not correct and that slowing of diffusion in cells is less marked than has been generally assumed...
  52. pmc Eukaryotic large nucleo-cytoplasmic DNA viruses: clusters of orthologous genes and reconstruction of viral genome evolution
    Natalya Yutin
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Virol J 6:223. 2009
    ....
  53. pmc Dis3-like 1: a novel exoribonuclease associated with the human exosome
    Raymond H J Staals
    Department of Biomolecular Chemistry, Nijmegen Center for Molecular Life Sciences, Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, The Netherlands
    EMBO J 29:2358-67. 2010
    ..In contrast to the nuclear localization of Dis3, hDis3L1 exclusively localized to the cytoplasm. The hDis3L1 isolated from transfected cells degraded RNA in an exoribonucleolytic manner, and its RNB domain ..
  54. pmc Anomalous subdiffusion is a measure for cytoplasmic crowding in living cells
    Matthias Weiss
    MEMPHYS Center for Biomembrane Physics, Physics Department, University of Southern Denmark, Campusvej 55, Odense M, Denmark
    Biophys J 87:3518-24. 2004
    ..We therefore propose that the anomality of the diffusion can be used as a quantifiable measure for the crowdedness of the cytoplasm at the molecular scale.
  55. ncbi Maurer's clefts: a novel multi-functional organelle in the cytoplasm of Plasmodium falciparum-infected erythrocytes
    Michael Lanzer
    Abteilung Parasitologie, Hygiene Institut, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Int J Parasitol 36:23-36. 2006
    Discovered in 1902 by Georg Maurer as a peculiar dotted staining pattern observable by light microscopy in the cytoplasm of erythrocytes infected with the human malarial parasite Plasmodium falciparum, the function of Maurer's clefts ..
  56. pmc Genome barriers between nuclei and mitochondria exemplified by cytoplasmic male sterility
    Sota Fujii
    Graduate School of Agricultural Science, Tohoku University, Sendai, 981 8555 Japan
    Plant Cell Physiol 49:1484-94. 2008
    ..This review summarizes the current perspectives in CMS and fertility restoration, mainly from the viewpoint of anterograde/retrograde signaling...
  57. ncbi Intermediate filaments: structure, dynamics, function, and disease
    E Fuchs
    Howard Hughes Medical Institute, University of Chicago, Illinois 60637
    Annu Rev Biochem 63:345-82. 1994
  58. pmc The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions
    H Le Hir
    Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, 415 South Street, Waltham, MA 02454, USA
    EMBO J 19:6860-9. 2000
    ..Possible functions for this complex in nucleocytoplasmic transport of spliced mRNA, as well as the nonsense-mediated mRNA decay pathway, are discussed...
  59. pmc Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
    Van Dat Nguyen
    Department of Biochemistry, Linnanmaa campus, University of Oulu, 90570 Oulu, Finland
    Microb Cell Fact 10:1. 2011
    ..We have recently shown that it is possible to produce large amounts of prokaryotic disulfide bond containing proteins in the cytoplasm of wild-type bacteria such as E. coli by the introduction of catalysts for both of these processes.
  60. ncbi Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly
    M L Luo
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 413:644-7. 2001
    ..These data suggest that the splicing factor UAP56 functions in coupling the splicing and export machineries by recruiting Aly to the spliced mRNP...
  61. pmc Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation
    Atsushi Iwata
    Department of Biological Sciences, Bio X Program, Stanford University, Stanford, CA 94305 5430
    Proc Natl Acad Sci U S A 102:13135-40. 2005
    ..These data indicate that autophagy is a critical component of the cellular clearance of toxic protein aggregates and may help to explain why protein aggregates are more toxic when directed to the nucleus...
  62. pmc Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells
    Giulia Biffi
    Cancer Research UK Cambridge Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Nat Chem 6:75-80. 2014
    ..Here, we demonstrate the visualization of RNA G-quadruplex structures within the cytoplasm of human cells using a G-quadruplex structure-specific antibody...
  63. ncbi The SMN-SIP1 complex has an essential role in spliceosomal snRNP biogenesis
    U Fischer
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia 19104 6148, USA
    Cell 90:1023-9. 1997
    ..Here we show that the SMN-SIP1 complex is associated with spliceosomal snRNAs U1 and U5 in the cytoplasm of Xenopus oocytes...
  64. pmc HDAC4 deacetylase associates with and represses the MEF2 transcription factor
    E A Miska
    Wellcome CRC Institute, Department of Pathology, Cambridge, UK
    EMBO J 18:5099-107. 1999
    ..We show that HDAC4, unlike other deacetylases, shuttles between the nucleus and the cytoplasm in a process involving active nuclear export...
  65. pmc Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo
    P GONCZY
    European Molecular Biology Laboratory, Heidelberg, D 69117 Germany
    J Cell Biol 147:135-50. 1999
    ..In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation...
  66. pmc Selective uncoupling of p120(ctn) from E-cadherin disrupts strong adhesion
    M A Thoreson
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175, USA
    J Cell Biol 148:189-202. 2000
    ..beta-catenins, p120 was metabolically stable in cadherin-deficient cells, and was present at high levels in the cytoplasm. Analysis of cells expressing E-cadherin mutant constructs indicated that p120 is required for the E-cadherin-..
  67. pmc TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm
    A Vassilev
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Genes Dev 15:1229-41. 2001
    ..However, whereas TEAD was concentrated in the nucleus, excess YAP65 accumulated in the cytoplasm as a complex with the cytoplasmic localization protein, 14-3-3...
  68. pmc The yeast endosomal Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking
    Christopher L Brett
    Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 16:1396-405. 2005
    ..This presents the newly defined family of eukaryotic endosomal NHE as novel targets for pharmacological inhibition to alleviate pathological states associated with organellar alkalinization...
  69. pmc A complete mitochondrial genome sequence of Ogura-type male-sterile cytoplasm and its comparative analysis with that of normal cytoplasm in radish (Raphanus sativus L.)
    Yoshiyuki Tanaka
    31 Laboratory, Kyoto Sangyo University, Motoyama, Kamigamo, Kita ku, Kyoto 603 8555, Japan
    BMC Genomics 13:352. 2012
    ..The Ogura-type male-sterile cytoplasm is one of the most extensively studied cytoplasms in Brassicaceae...
  70. pmc The epididymis, cytoplasmic droplets and male fertility
    Trevor G Cooper
    Centre of Reproductive Medicine and Andrology, University of Munster, Munster D 48149, Germany
    Asian J Androl 13:130-8. 2011
    ..This may reflect their short midpiece, approximating head length, permitting a swollen droplet to extend along the entire midpiece; this not only obviates droplet migration and flagellar angulation but also hampers droplet loss...
  71. ncbi Redox regulation of NF-kappaB activation: distinct redox regulation between the cytoplasm and the nucleus
    Yasuaki Kabe
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta cho, Midori ku, Yokohama 226 8501, Japan
    Antioxid Redox Signal 7:395-403. 2005
    ..NF-kappaB is trapped in the cytoplasm in stimulated cells and translocates into the nucleus in response to several stimuli, including oxidative stress...
  72. pmc A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes
    Taisuke Yano
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, Japan
    Mol Cell Biol 30:3758-66. 2010
    ....
  73. pmc Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm
    Manli Qi
    Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA
    J Bacteriol 193:2498-509. 2011
    ..The endogenous CT795 was detected in the cytoplasm of host cells during C...
  74. pmc Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export of metazoan PABPs
    Hannah M Burgess
    MRC Centre for Reproductive Health MRC Human Reproductive Sciences Unit, Queens Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, UK
    J Cell Sci 124:3344-55. 2011
    ..These data also provide mechanistic insight into reports that transcriptional inhibitors and expression of certain viral proteins cause relocation of PABP to the nucleus...
  75. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
    ..We suggest that p62 may, via LC3, be involved in linking polyubiquitinated protein aggregates to the autophagy machinery...
  76. ncbi Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila
    A Wodarz
    Institut fur Entwicklungsbiologie, Universitat zu Koln, Federal Republic of Germany
    Cell 82:67-76. 1995
    ..Our results suggest that crumbs plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells of Drosophila...
  77. pmc MicroRNA maturation: stepwise processing and subcellular localization
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul 151 742, Korea
    EMBO J 21:4663-70. 2002
    ..localization studies showed that the first and second steps are compartmentalized into the nucleus and cytoplasm, respectively, and that the pre-miRNA serves as the substrate for nuclear export...
  78. pmc The nuclear protein HMGB1 is secreted by monocytes via a non-classical, vesicle-mediated secretory pathway
    Stefania Gardella
    National Cancer Research Institute, Genoa, Italy
    EMBO Rep 3:995-1001. 2002
    ..Thus, in monocytes, non-classical secretion can occur through vescicle compartments that are at least partially distinct...
  79. pmc Coupling of the nucleus and cytoplasm: role of the LINC complex
    Melissa Crisp
    Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610
    J Cell Biol 172:41-53. 2006
    The nuclear envelope defines the barrier between the nucleus and cytoplasm and features inner and outer membranes separated by a perinuclear space (PNS)...
  80. pmc Single-molecule analysis of dynein processivity and stepping behavior
    Samara L Reck-Peterson
    Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA
    Cell 126:335-48. 2006
    ....
  81. pmc Diffusion, crowding & protein stability in a dynamic molecular model of the bacterial cytoplasm
    Sean R McGuffee
    Department of Biochemistry, University of Iowa, Iowa City, Iowa, United States of America
    PLoS Comput Biol 6:e1000694. 2010
    ..Brownian dynamics (BD) simulations of the cytoplasm model have been calibrated to reproduce the translational diffusion coefficients of Green Fluorescent Protein (..
  82. pmc Mechanism for alternating access in neurotransmitter transporters
    Lucy R Forrest
    Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics and Department of Biochemistry and Molecular Biophysics, Columbia University, 1130 St Nicholas Avenue, Room 815, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 105:10338-43. 2008
    ..show a molecule of bound substrate that is essentially exposed to the extracellular space but occluded from the cytoplasm. Thus, there must exist an alternate conformation for LeuT in which the substrate is accessible to the cytoplasm ..
  83. pmc Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways
    J Kim
    Section of Microbiology, University of California, Davis, California 95616, USA
    Mol Biol Cell 10:1337-51. 1999
    ..machinery as a biosynthetic pathway for the delivery of the vacuolar hydrolase, aminopeptidase I, via the cytoplasm-to-vacuole targeting (Cvt) pathway...
  84. pmc On the mechanism of sensing unfolded protein in the endoplasmic reticulum
    Joel J Credle
    Howard Hughes Medical Institute, Departments of Biochemistry and Biophysics and Medicine, University of California, San Francisco, CA 94143 2200, USA
    Proc Natl Acad Sci U S A 102:18773-84. 2005
    ..The changes in the ER lumen in turn position Ire1 kinase domains in the cytoplasm optimally for autophosphorylation to initiate the UPR.
  85. ncbi A comprehensive model for the cellular uptake of cationic cell-penetrating peptides
    Falk Duchardt
    Interfaculty Institute for Cell Biology, University of Tubingen, Auf der Morgenstelle 15, 72076 Tubingen, Germany
    Traffic 8:848-66. 2007
    ..For Antp, significantly higher peptide concentrations and inhibition of endocytosis were required to induce its uptake. The relevance of these findings for import of biologically active cargos is shown...
  86. doi SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs
    Xiaoju Max Ma
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 133:303-13. 2008
    ..Thus, SKAR-mediated recruitment of activated S6K1 to newly processed mRNPs serves as a conduit between mTOR checkpoint signaling and the pioneer round of translation when cells exist in conditions supportive of protein synthesis...
  87. doi Structural determinants of the cellular localization and shuttling of TDP-43
    Youhna M Ayala
    International Centre for Genetic Engineering and Biotechnology ICGEB, 34012 Trieste, Italy
    J Cell Sci 121:3778-85. 2008
    ..We provide the first experimental evidence that TDP-43 continuously shuttles between nucleus and cytoplasm in a transcription-dependent manner...
  88. ncbi Exporting RNA from the nucleus to the cytoplasm
    Alwin Köhler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Nat Rev Mol Cell Biol 8:761-73. 2007
    The transport of RNA molecules from the nucleus to the cytoplasm is fundamental for gene expression. The different RNA species that are produced in the nucleus are exported through the nuclear pore complexes via mobile export receptors...
  89. pmc Bridging IRES elements in mRNAs to the eukaryotic translation apparatus
    Kerry D Fitzgerald
    Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA 92697, USA
    Biochim Biophys Acta 1789:518-28. 2009
    ....
  90. ncbi Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes
    Aki Fujioka
    Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    J Biol Chem 281:8917-26. 2006
    ..This prediction was confirmed experimentally by decreasing the level of Raf using the small interfering RNA technique. This observation verified the usefulness of the parameters collected in this study...
  91. pmc Expression of TDP-43 C-terminal Fragments in Vitro Recapitulates Pathological Features of TDP-43 Proteinopathies
    Lionel M Igaz
    Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine, USA
    J Biol Chem 284:8516-24. 2009
    ..Thus, our results show that TDP-43 CTF expression recapitulates key biochemical features of pathological TDP-43 and support the hypothesis that the generation of TDP-43 CTFs is an important step in the pathogenesis of FTLD-U and ALS...
  92. pmc Virulent and avirulent strains of Francisella tularensis prevent acidification and maturation of their phagosomes and escape into the cytoplasm in human macrophages
    Daniel L Clemens
    Division of Infectious Diseases, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095 1688, USA
    Infect Immun 72:3204-17. 2004
    ..tularensis bacteria lacked any ultrastructurally discernible membrane separating them from the host cell cytoplasm. These results indicate that F...
  93. pmc Late cytoplasmic maturation of the small ribosomal subunit requires RIO proteins in Saccharomyces cerevisiae
    Emmanuel Vanrobays
    Laboratoire de Biologie Moleculaire Eucaryote du CNRS, 118 route de Narbonne, 31062 Toulouse Cedex, France
    Mol Cell Biol 23:2083-95. 2003
    ..In situ hybridization reveals that in Rio2p-depleted cells, 20S pre-rRNA localizes in the cytoplasm, demonstrating that its accumulation is not due to an export defect...
  94. pmc hnRNP A1 interacts with the 5' untranslated regions of enterovirus 71 and Sindbis virus RNA and is required for viral replication
    Jing Yi Lin
    Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Tao Yuan, Taiwan
    J Virol 83:6106-14. 2009
    ..ribonucleoprotein (hnRNP) A1 is involved in pre-mRNA splicing in the nucleus and translational regulation in the cytoplasm. The cytoplasmic redistribution of hnRNP A1 is a regulated process during viral infection and cellular stress...
  95. doi Germline P granules are liquid droplets that localize by controlled dissolution/condensation
    Clifford P Brangwynne
    Max Planck Institute for Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    Science 324:1729-32. 2009
    ..This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell. Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasm.
  96. pmc Nuclear and cytoplasmic localization of neural stem cell microRNAs
    Clark D Jeffries
    Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    RNA 17:675-86. 2011
    Although generally regarded as functional in the cytoplasm, a number of microRNAs (miRNAs) have been found in the nucleus, possibly with a role in gene regulation...
  97. doi The sarcolipin-bound calcium pump stabilizes calcium sites exposed to the cytoplasm
    Anne Marie L Winther
    Pcovery, Thorvaldsensvej 57, DK 1871 Frederiksberg, Denmark
    Nature 495:265-9. 2013
    ..These findings may prove useful in studying how autoinhibitory domains of other ion pumps modulate transport across biological membranes...
  98. pmc Gradual processing of the ITS1 from the nucleolus to the cytoplasm during synthesis of the human 18S rRNA
    Milena Preti
    Laboratoire de Biologie Moleculaire Eucaryote, Universite de Toulouse, UPS, F 31000 Toulouse, France
    Nucleic Acids Res 41:4709-23. 2013
    ..These data cast new light on the original mechanism of ITS1 elimination in human cells and provide a mechanistic framework to further study the interplay of DBA-linked ribosomal proteins in this process...
  99. ncbi Wolbachia establishment and invasion in an Aedes aegypti laboratory population
    Zhiyong Xi
    Department of Entomology, University of Kentucky, Lexington, KY 40546, USA
    Science 310:326-8. 2005
    ..Laboratory cage tests demonstrated the ability of wAlbB to spread into an A. aegypti population after seeding of an uninfected population with infected females, reaching infection fixation within seven generations...
  100. pmc Artificial triple Wolbachia infection in Aedes albopictus yields a new pattern of unidirectional cytoplasmic incompatibility
    Yuqing Fu
    Department of Entomology, University of Kentucky, Lexington, KY 40546, USA
    Appl Environ Microbiol 76:5887-91. 2010
    ..This unidirectional CI, combined with a high fidelity of maternal inheritance and low fecundity effects, suggests that the artificial cytotype could serve as an appropriate vehicle for gene drive...
  101. ncbi From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion
    R Kemler
    Max Planck Institut fur Immunbiologie, Freiburg, Germany
    Trends Genet 9:317-21. 1993
    ..Catenins are part of a higher order protein structure that is of crucial importance for the adhesive function of cadherins. A working model of the construction and regulation of this multiprotein interaction is proposed...

Research Grants76

  1. Genetic variation and regulatory networks: Mechanisms and complexity
    DANA PE ER; Fiscal Year: 2009
    ..These tools will be made publicly available, including a friendly graphical user interface and visualization. ..
  2. Enhanced iron removal with peptide linked chelators targeted to ferritin
    Elizabeth C Theil; Fiscal Year: 2010
    ..The current model posits that closed pores in ferritin prevent reductant (in solution or the cytoplasm) from reacting with the ferritin mineral...
  3. GENETIC ANALYSIS OF POLARITY
    RUTH M STEWARD; Fiscal Year: 2010
    ..In some lymphomas, PDCD2 is present in the cytoplasm of normal lymphocytes, but is not detected in malignant lymphocytes, indicating that the gene may have similar ..
  4. BIOCHEMISTRY OF PRE-MRNA SPLICING
    Adrian R Krainer; Fiscal Year: 2010
    ..exons from primary transcripts in the nucleus to generate mature mRNA, the template for protein synthesis in the cytoplasm. Many genes express multiple mRNA and protein isoforms by alternative splicing of their primary transcripts...
  5. Scott J Hultgren; Fiscal Year: 2015
    ..Murine models indicate that after invasion, UPEC can subvert innate expulsion mechanisms and escape into the cytoplasm where rapid bacterial replication results in the formation of intracellular bacterial communities (IBC) ..
  6. Cellular and Animal Models of FUS Mutations in ALS
    Eric J Huang; Fiscal Year: 2013
    ..the spinal motor neurons in patients with P525L mutation show mislocalization of mutant FUS proteins in neuronal cytoplasm with abnormal FUS protein aggregates that are associated with markedly disorganized intracellular organelles, ..
  7. Nuclear-Cytoplasmic Shuttling of Foxc2 via 14-3-3 Determines Proximal Tubule Cell
    DIANA LYNNE GOLDEN; Fiscal Year: 2012
    ..I have also discovered that Foxc2 associates with 14- 3-3 and [unreadable]-actinins in the cytoplasm of mouse proximal tubule cells...
  8. Preclinical drug trial in mouse models of inflammation
    Kanneboyina Nagaraju; Fiscal Year: 2011
    ..effects are due to GC-glucocorticoid receptor (GR) mediated transrepressive (NF-kB inhibition) activities in cytoplasm and side effects (metabolic and hormonal) are thought to be predominantly mediated by transactivation via GC ..
  9. Intracellular Serpin Regulation of Intestinal Cell Necrosis
    GARY ARTHUR SILVERMAN; Fiscal Year: 2012
    ..Recently, we identified a subset of serpins that are abundantly expressed within the cytoplasm of metazoan epithelial cells, including those of the mammalian gastrointestinal tract...
  10. Christin Carter-Su; Fiscal Year: 2016
    ..g., focal adhesions, lamellipodia, cytoplasm, nucleus)...
  11. Roles of NF-kB/Rel in the pathogenesis of breast cancer
    Gail E Sonenshein; Fiscal Year: 2012
    ..NF-?B factors are sequestered in the cytoplasm in an inactive complex in almost all non-B cells...
  12. Mark R Plummer; Fiscal Year: 2014
    ..PUBLIC HEALTH RELEVANCE: The dynamic behavior of the ATM protein in the neuronal cytoplasm and the discovery of its intimate association with proteins involved in synaptic vesicle behavior argue that ..
  13. Juanita L Merchant; Fiscal Year: 2014
    ..In the current proposal, pATMS1981 positive expression in the gut was found to occur exclusively in the cytoplasm of intestinal EC cells suggesting that pATMS1981 participates in an essential function of these cells...
  14. Kanneboyina Nagaraju; Fiscal Year: 2015
    ..effects are due to GC-glucocorticoid receptor (GR) mediated transrepressive (NF-kB inhibition) activities in cytoplasm and side effects (metabolic and hormonal) are thought to be predominantly mediated by transactivation via GC ..
  15. FASEB SRC on Mitochondrial Assembly &Dynamics in Health, Disease &Aging
    Antoni Barrientos; Fiscal Year: 2011
    ..mitochondrial biogenesis and function, mitochondrial DNA maintenance, mitochondrial-nuclear and mitochondrial-cytoplasm cross talk, mitochondrial protein sorting, assembly of individual respiratory complexes and cofactor biogenesis...
  16. Anthrax Toxins Impair Phagocyte Actin-based Motility
    Frederick Southwick; Fiscal Year: 2010
    ..toxins, protective antigen (PA) combined with lethal factor (LF) and edema factor (EF) are able to enter the cytoplasm of phagocytic cells and impair their function...
  17. DAVID ANTONETTI; Fiscal Year: 2016
    ..VEGF induces redistribution of occludin and other tight junction proteins from the plasma membrane to the cell cytoplasm and over time, leads to the degradation of occludin...
  18. Dana Boyd; Fiscal Year: 2014
    ..In certain archaea, we have obtained evidence for another unusual pathway for disulfide bond formation in the cytoplasm. These archaea contain two VKORs, one cytoplasmically-oriented and apparently involved in cytoplasmic disulfide ..
  19. Nicola C Partridge; Fiscal Year: 2016
    ..of Runx2 and HDAC4, resulting in the release of HDAC4 from the MMP-13 promoter and its trafficking to the cytoplasm. In the nucleus, Runx2 then recruits the histone acetyl transferases (HATs), p300 and p300/CBP associated factor ..
  20. Jennifer S Stone; Fiscal Year: 2014
    ..hair cell - type II - in adult rodents has a feature that has never been described: one or more extensions of cytoplasm (or processes) that project laterally from the base of the cell, sometimes over several cell lengths...
  21. Molecular Regulation of Alpha-Synuclein Misfolding and Toxicity in Two Yeast Mode
    SHUBHIK DEBBURMAN; Fiscal Year: 2009
    ..In fission yeast, a-synuclein misfolds and aggregates within the cytoplasm in an exquisitely time and concentration-dependent manner, providing crucial live cell evidence for a mechanism ..
  22. Enzymology of Chlamydia Pathogenesis
    Dewey G McCafferty; Fiscal Year: 2013
    ..During infection, C. trachomatis releases numerous effector molecules into the host cell cytoplasm to interfere with host signaling, which aids in chlamydial cooption of key host cell functions that benefit the ..
  23. NANCY DEMORE; Fiscal Year: 2014
    ..Activated calcineurin dephosphorylates NFAT, which then translocates from the cytoplasm to the nucleus and results in transcription of genes involved in cell growth, differentiation, and cell cycle ..
  24. ClC-3 Chloride Ion Channels in Vascular Smooth Muscle
    Fred Lamb; Fiscal Year: 2009
    ..Aim #3 will determine if O2-" or H2O2 exit endosomes and reach the cytoplasm via regulated channels...
  25. Robert Harris; Fiscal Year: 2015
    ..We have cloned the human NMNAT2 gene that is expressed in the cytoplasm, transfected it into colorectal cancer cells and demonstrated that cell-kill is enhanced with Tiazofurin...
  26. John K Buolamwini; Fiscal Year: 2015
    ..Mitochondrial membrane nucleoside transporters (NTs) import NRTIs from the cytoplasm. The NT isoforms that have been implicated in mitochondrial nucleoside uptake are the equilibrative nucleoside ..
  27. Autophagy in Brain Tumor
    Jin Ming Yang; Fiscal Year: 2013
    ..that autophagy, a conserved response to nutrient/growth factor deprivation and a process of self-digestion of cytoplasm and organelles through which cellular components are recycled for energy utilization, may represent a critical ..
  28. Dennis L Stevens; Fiscal Year: 2016
    ..This suggests that the ATP-driven enzyme that removes calcium from the cytoplasm is adversely affected by SLO...
  29. Role of Meprin A in Acute Kidney Injury
    GUR PRASAD KAUSHAL; Fiscal Year: 2013
    ..Following ischemia-reperfusion (IR)- and cisplatin-induced AKI, meprin A is redistributed throughout the cytoplasm and extracellularly adhering toward the basolateral plasma membrane and the cleaved form of meprin A is excreted ..
  30. METABOLIC CONTROL OF SUGAR TRANSPORT
    Anthony Carruthers; Fiscal Year: 2012
    ..Sugar release from the cage into cytoplasm is much slower and is further inhibited by ATP which restructures GLUT1 loop 6, exofacial loop 7 and the C-..
  31. GERTRUD M SCHUPBACH; Fiscal Year: 2016
    ..defects caused by retrotransposons, activate a meiotic checkpoint that controls translation of Grk in the oocyte cytoplasm. Our goal is to study the regulation of Gurken production in the germline and to analyze the patterning and ..
  32. Molecular Mechanism of cell-cell spread of Listeria in polarized epithelial cells
    KEITH PATRICK IRETON; Fiscal Year: 2013
    ..protein ActA, which stimulates the formation of F-actin 'comet tails'that propel Listeria through the cytoplasm. Rocketing bacteria then contact the host cell plasma membrane and induce the formation of Listeria-containing ..
  33. Regulation of Hepatic Gluconeogenesis by the CREB:TORC2 Pathway
    Marc R Montminy; Fiscal Year: 2010
    ..Sequestered in the cytoplasm during feeding, TORC2 translocates to the nucleus in response to fasting signals, where it triggers ..
  34. Marc R Montminy; Fiscal Year: 2016
    ..Sequestered in the cytoplasm during feeding, TORC2 translocates to the nucleus in response to fasting signals, where it triggers ..
  35. Haining Zhu; Fiscal Year: 2014
    ..thus hypothesize that the ALS-related FUS mutants or WT FUS with deregulated over-expression can accumulate in cytoplasm and sequester Gemin3, which results in decreased assembly of snRNPs in cytoplasm and compromised spliceosome ..
  36. Fabeha Fazal; Fiscal Year: 2014
    ..Activation of RelA/p65 requires its release from the inhibitory protein I(B( in the cytoplasm and subsequently, its translocation to the nucleus...
  37. Encapsidation of the poxvirus genome
    Paula Traktman; Fiscal Year: 2012
    ..by applicant): Poxviruses, such as vaccinia virus, are unique among DNA viruses in replicating solely within the cytoplasm of the infected cell...
  38. Heat Shock-Induced Apoptosis
    Shawn B Bratton; Fiscal Year: 2012
    ..permeabilization (ELMP), which coincides with cytosolic acidification and release of cathepsins into the cytoplasm, both of which participate in the processing of procaspase-3 and the proapoptotic BH3-only protein Bid...
  39. Targeting Glucose Metabolism in Cancer
    Sucheta Telang; Fiscal Year: 2013
    ..Further, cell fractionation revealed that the PFKFB4 protein, and not PFKFB3, localized to the cytoplasm, the cellular compartment of both PFK-1 and glycolysis...
  40. Eileen P White; Fiscal Year: 2016
    ..of autophagy cargo such as amino acids, sugars, nucleosides and lipids, are released from lysosomes into the cytoplasm where they are reused...
  41. Iron Acquisition by Mycobacterium tuberculosis Within Phagocytes
    Bradley E Britigan; Fiscal Year: 2013
    ..We find that M.tb in human macrophages can acquire Fe from TF and LF, as well as the macrophage cytoplasm. Fe uptake from extracellular TF and LF may involve Fe moving into the cytoplasm. Some (e.g...
  42. The in vivo role of the mitochondrial p53 cell death program
    UTE MARTHA MOLL; Fiscal Year: 2013
    ..Rather than the nucleus, the cytoplasm contains a separate and distinct p53 pool that becomes stress-stabilized and serves as the major source for p53 ..
  43. Growth Hormone Regulation of SH2-B1
    Christin Carter Su; Fiscal Year: 2009
    ..Finally, SH2B1 shuttles between the plasma membrane, cytoplasm and nucleus...
  44. Cthrc1 function as a novel TGF-beta antagonist in the vasculature
    Volkhard Lindner; Fiscal Year: 2013
    ..Cthrc1 has an unexpected biogenesis because as it contains a signal peptide for secretion yet remains in the cytoplasm of differentiated smooth muscle cells (SMC)...
  45. Novel Role of a Nucleoporin Gene in Atrial Fibrillation, the Most Common Cardiac
    Qing Kenneth Wang; Fiscal Year: 2013
    ..Export of mRNA from the nucleus to the cytoplasm plays an important role in gene expression in eukaryotic cells...
  46. WILLIAM S WARD; Fiscal Year: 2014
    ..and our preliminary data suggest that initiation of DNA synthesis of two pronuclei in the same oocyte cytoplasm can vary by as much as three hours...
  47. TR4 orphan receptor, a longevity assurance gene, in prostate carcinogenesis
    Yi Fen Lee; Fiscal Year: 2012
    ..of TR4 profiles from human prostate samples found TR4 expressions were increased and shifted from the nucleus to cytoplasm proportionally with the progression of disease, then declined and appeared only in the cytoplasm of specimens of ..
  48. Histone proline isomerization and gene regulation
    Nobuaki Kikyo; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Existence of powerful nuclear reprogramming activities in egg cytoplasm has been unequivocally demonstrated by successful somatic cell nuclear cloning using terminally differentiated cell nuclei as ..
  49. Role of mineralocorticoid receptor in primate ovarian function
    CHARLES CHAFFIN; Fiscal Year: 2009
    ..MR is expressed in the cytoplasm of both luteinizing granulosa cells as well as the corpus luteum until the mid to mid-late luteal phase, at ..
  50. Toxoid adjuvant CRM197 production in a stable reduced genome E. coli strain
    Frederick R Blattner; Fiscal Year: 2012
    ..Alternatively, CRM197 produced at high levels in the cytoplasm, will be subjected to contemporary refolding methods to regenerate the soluble form...
  51. Ping Wang; Fiscal Year: 2016
    ..By using GFP-CIRP expression plasmid, we were able to show CIRP translocation from the nucleus to cytoplasm under hypoxic conditions. We have determined that TLR4, rather than TLR2 or RAGE, serves as CIRP receptor...
  52. James A Imlay; Fiscal Year: 2015
    ..that cystine import is linked to reduction, a tacti that would avoid the release of this disulfide into the cytoplasm. In Aim 2 this model will be rigorously tested...
  53. Role of an RNA-Binding Protein in Mammary Carcinogenesis
    Jeffrey Ross; Fiscal Year: 2009
    ..This protein is located in the cytoplasm and binds to insulin-like growth factor-ll, p-actin, c-myc, and other mRNAs...
  54. Sanja Sever; Fiscal Year: 2016
    ..forms of proteinuria and nephrotic syndrome are characterized by transformation of podocyte FPs into a band of cytoplasm due to de- regulation of the actin cytoskeleton (referred to as FP effacement)...
  55. Alan P Fields; Fiscal Year: 2016
    ..During the current funding period we showed that: 1) PKC? forms an oncogenic PKC?/Par6 signaling complex in the cytoplasm of NSCLC cells that is necessary for cell proliferation and invasion in vitro, and tumor formation in vivo;2) ..
  56. EMILY YIN CHU; Fiscal Year: 2014
    ..predicted to act as a transcription factor, although studies in keratinocytes have found IRF6 primarily in the cytoplasm. Recent studies in the Cox laboratory have found that IRF6 binds, in a phosphorylation-enhanced manner, to NME2 (..
  57. WILLIAM RAMSES BISHAI; Fiscal Year: 2014
    ..tuberculosis uses at least one of its adenylate cyclases (Rv0386) to secrete cAMP into the host cell cytoplasm. By this cAMP intoxication mechanism, M...
  58. Sandra L Wolin; Fiscal Year: 2016
    ..In the cytoplasm, Ro binds noncoding RNAs called Y RNAs that regulate the subcellular distribution of Ro...
  59. RAIMOND LESTER WINSLOW; Fiscal Year: 2014
    ..Recent evidence indicates that alterations in the reduction- oxidation (redox) potential of the cytoplasm, sarcoplasmic reticulum, and the mitochondria of the heart may be a key factor involved in the progression of ..
  60. Gender-Specific Role of Aim2 in Inflammation and Autoimmunity
    Divaker Choubey; Fiscal Year: 2011
    ..IFN)-inducible p200-protein family, have been identified as novel sensors of double-stranded DNA (dsDNA) in the cytoplasm. The HIN-200 domain of the Aim2 protein binds to dsDNA, while the pyrin (PYD) domain associates with the adapter ..
  61. Alpha actinin 4: its functions and regulation
    Hung Ying Kao; Fiscal Year: 2013
    ..Based on our preliminary data, we hypothesize that ACTN4 plays a role in both the nucleus and the cytoplasm and that ACTN4 is capable of modulating transcriptional activity of nuclear receptors in the nucleus of ..
  62. Regulation of Cell Growth by RNA Binding Proteins
    Nikolai A Timchenko; Fiscal Year: 2012
    ..and cyclin D3, which are activators of cdk4, are increased in cytoplasmic extracts of human liver tumors and in cytoplasm of mouse liver proliferating after partial hepatectomy...
  63. Evaluation of RNA transport elements in HIV-1 vectors
    SRINIVAS KUMAR; Fiscal Year: 2007
    ..regulatory elements (PTREs) effect various aspects of RNA function: transport of RNAs from the nucleus to the cytoplasm, localization of the RNA in specific cellular compartments, association with ribosomes, and translation...
  64. The effect of pncA expression and POA efflux rate in the level of PZA-resistance
    Mirko J Zimic; Fiscal Year: 2010
    ..The protoned molecule returns to the bacilli passively and release a protonwhich cause cytoplasm acidification and lethal disruption of the membrane transport...
  65. THE GASTRIC ACID PUMP AS A TARGET FOR ULCER TREATMENT
    George Sachs; Fiscal Year: 2009
    ..We plan to delineate pathways for transport of hydronium ion from cytoplasm to lumen and K+ from lumen to cytoplasm by analyzing various enzyme activities including phosphorylation and ..
  66. Probing localiztion, interactions, and effector properties of Shigella lpaD
    NICHOLAS EUGENE DICKENSON; Fiscal Year: 2010
    ..senses host cell contact and helps to recruit downstream effector proteins for injection into the membrane and cytoplasm of the host cell to promote bacterial invasion...
  67. Human Zinc Transporter Zip 13 and Cellular Zinc Homeostasis
    Joel Walker; Fiscal Year: 2007
    ..involves the Zrt-, Irt-like, Protein (ZIP) family of zinc transporters which function to import zinc into the cytoplasm. This research proposal addresses the role of the human transporter Zip13 (hZip13) in maintaining zinc ..
  68. Real-time microviscosity measurement tools for the cell
    Mark A Haidekker; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Microviscosity in the cell membrane and the cell cytoplasm are important parameters in cell signaling and disease...
  69. Transgenesis-Ready Mice with Tn5 Transposase
    JOANNE EBESU; Fiscal Year: 2007
    ..injection (ROSI), but was not very successful with injections of the Tn5 protein (Tn5p) DNA complex into the cytoplasm or pronuclei of fertilized single cell embryos...
  70. Role of ER-Localized Chaperones in Toxin Pathogenesis
    David Haslam; Fiscal Year: 2006
    ..From the ER lumen, the toxin must gain access to the cytoplasm, where it enzymatically inactivates the 28S ribosomal RNA, inhibiting protein synthesis...
  71. Cytoplasmic Reactivation of p53 Family Members
    Charles Walker; Fiscal Year: 2004
    ..we have identified for human p53 and p63/73 proteins are rendered non-functional by their sequestration in the cytoplasm of leukemic clam hemocytes...
  72. STEROID RECEPTOR TRANSFORMATION
    WILLIAM PRATT; Fiscal Year: 2005
    ..proteins, such as steroid receptors, STAT proteins or MAP kinases, move in precisely targeted manner through the cytoplasm to their nuclear sites of action, and this constitutes a fundamental gap in the modeling of signaling pathways ..
  73. LISTERIA MONOCYTOGENES AND PHAGOSOME MEMBRANE TRAFFIC
    Philip Stahl; Fiscal Year: 2003
    ..Virulent LM escapes to the cytoplasm. Listeria mutants (LMhly-) that lack listeriolysin fail to access the cytoplasm but retain the ability block ..
  74. PERMEABILITY OF THE EPIDEMIC TYPHUS RICKETTSIA
    JONATHON PETER AUDIA; Fiscal Year: 2010
    ..prowazekii, is an obligate intracellular bacterial parasite;rickettsiae only grow directly in the cytoplasm of hosts and vectors...