Genomes and Genes
Summary: Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
Publications276 found, 100 shown here
- The nucleolus under stressSÃ©verine Boulon
Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK
Mol Cell 40:216-27. 2010..Crosstalk between nucleoli and CBs is also discussed in the context of stress responses...
- Driving ribosome assemblyDieter Kressler
Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
Biochim Biophys Acta 1803:673-83. 2010..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
- Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotypeBrunangelo Falini
Institute of Hematology, University of Perugia, Perugia, Italy
N Engl J Med 352:254-66. 2005..Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein...
- Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11Masato Sasaki
Global Centers of Excellence Program, Akita University Graduate School of Medicine, Akita, Japan
Nat Med 17:944-51. 2011..When PICT1 is depleted in tumor cells with intact P53 signaling, the cells grow more slowly and accumulate P53. Thus, PICT1 is a potent regulator of the MDM2-P53 pathway and promotes tumor progression by retaining RPL11 in the nucleolus...
- Epigenetic control of rDNA loci in response to intracellular energy statusAkiko Murayama
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8572, Japan
Cell 133:627-39. 2008..These findings provide key insight into the mechanisms of energy homeostasis in cells...
- Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteinsYun Wah Lam
Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Curr Biol 17:749-60. 2007....
- CAG expansion induces nucleolar stress in polyglutamine diseasesHo Tsoi
Laboratory of Drosophila Research, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Proc Natl Acad Sci U S A 109:13428-33. 2012..Our work provides in vivo evidence that expanded CAG RNAs trigger nucleolar stress and induce apoptosis via p53 and describes a polyQ pathogenic mechanism that involves the nucleolus...
- Ribosome biogenesis surveillance: probing the ribosomal protein-Mdm2-p53 pathwayC Deisenroth
Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Oncogene 29:4253-60. 2010..In this review, we will clarify how disruption to three major components of ribosome biogenesis can trigger nucleolar stress and activate p53, thereby lending support to a RP-Mdm2-p53 ribosome biogenesis surveillance pathway...
- Nucleolus, ribosomes, and cancerLorenzo Montanaro
Department of Experimental Pathology, University of Bologna, Bologna, Italy
Am J Pathol 173:301-10. 2008....
- Poly(ADP-Ribose) polymerase 1 (PARP-1) regulates ribosomal biogenesis in Drosophila nucleoliErnest K Boamah
Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
PLoS Genet 8:e1002442. 2012..Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis...
- Characterization and prediction of protein nucleolar localization sequencesMichelle S Scott
Division of Biological Chemistry and Drug Discovery and Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Nucleic Acids Res 38:7388-99. 2010..NoLSs are a prevalent type of targeting motif that is distinct from nuclear localization signals and that can be computationally predicted...
- Nuclear neighborhoods and gene expressionRui Zhao
Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794, USA
Curr Opin Genet Dev 19:172-9. 2009..We are still at a very early, yet exciting stage in our journey toward deciphering the mechanism(s) that govern(s) the permissiveness of gene expression/repression within different nuclear neighborhoods...
- Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesisDenis Drygin
Cylene Pharmaceuticals, Inc, San Diego, CA 92121, USA
Cancer Res 69:7653-61. 2009..CX-3543 is the first G-quadruplex interactive agent to enter human clinical trials, and it is currently under evaluation against carcinoid/neuroendocrine tumors in a phase II clinical trial...
- Directed proteomic analysis of the human nucleolusJens S Andersen
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
Curr Biol 12:1-11. 2002..Recent studies suggest it may also have roles in RNA transport, RNA modification, and cell cycle regulation. Despite over 150 years of research into nucleoli, many aspects of their structure and function remain uncharacterized...
- Nucleophosmin (B23) targets ARF to nucleoli and inhibits its functionChandrashekhar Korgaonkar
Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City, IA 52242, USA
Mol Cell Biol 25:1258-71. 2005..Conversely, NPM overexpression antagonized ARF function while increasing its nucleolar localization. These data suggest that NPM inhibits ARF's p53-dependent activity by targeting it to nucleoli and impairing ARF-Mdm2 association...
- CSB is a component of RNA pol I transcriptionJohn Bradsher
Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, F 67404, Illkirch Cedex, France
Mol Cell 10:819-29. 2002..In addition to revealing an unanticipated function for CSB in rRNA synthesis, we show that the fragility of this complex could be one factor contributing to the CS phenotype...
- Small nucleolar RNAs: an abundant group of noncoding RNAs with diverse cellular functionsTamas Kiss
Laboratoire de Biologie Moleculaire Eucaryote du CNRS, Universite Paul Sabatier, 118 route de Narbonne, 31062 Cedex, Toulouse, France
Cell 109:145-8. 2002....
- Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated exportH Grosshans
Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
J Cell Biol 153:745-62. 2001..This particle can then be targeted to the nuclear pores and is subsequently exported to the cytoplasm in an Xpo1p-dependent way...
- RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycleBenjamin Albert
Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Universite de Toulouse, F 31000 Toulouse, France
J Cell Biol 192:277-93. 2011..Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly...
- Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complexNicholas J Watkins
Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, D 37070 Gottingen, Germany
Mol Cell 16:789-98. 2004..We believe that the assembly complex coordinates snoRNA processing, snoRNP assembly, restructuring, and localization...
- The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during developmentAlexei Tulin
Howard Hughes Medical Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
Genes Dev 16:2108-19. 2002..We propose that PARP-e autoregulates Parp transcription by influencing the chromatin structure of its heterochromatic environment. Our results indicate that Parp plays a fundamental role organizing the structure of Drosophila chromatin...
- Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradationSari Kurki
Haartman Institute and Molecular Cancer Biology Research Program, Biomedicum Helsinki, University of Helsinki, P O Box 63, FIN 00014 Helsinki, Helsinki, Finland
Cancer Cell 5:465-75. 2004..The results demonstrate novel associations of HDM2 and K cyclin with NPM and implicate NPM as a crucial controller of p53 through inhibition of HDM2...
- Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibitionYaron Shav-Tal
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100 Israel
Mol Biol Cell 16:2395-413. 2005....
- Modulation of RNA editing by functional nucleolar sequestration of ADAR2Christopher L Sansam
Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
Proc Natl Acad Sci U S A 100:14018-23. 2003..These observations indicate that the nucleolar localization of ADAR2 represents an important mechanism by which RNA editing can be modulated by the sequestration of enzymatic activity from potential RNA substrates in the nucleoplasm...
- Inhibition of homologous recombination by a cohesin-associated clamp complex recruited to the rDNA recombination enhancerJulie Huang
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Genes Dev 20:2887-901. 2006....
- Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromereLee H Wong
Chromosome and Chromatin Research Laboratory, Murdoch Children s Research Institute and Department of Paediatrics, University of Melbourne, Royal Children s Hospital, Parkville, Victoria, Australia
Genome Res 17:1146-60. 2007....
- Intergenic transcripts regulate the epigenetic state of rRNA genesChristine Mayer
German Cancer Research Center, Division of Molecular Biology of the Cell II, D 69120 Heidelberg, Germany
Mol Cell 22:351-61. 2006..The results reveal an important contribution of processed IGS transcripts in chromatin structure and epigenetic control of the rDNA locus...
- Birth of a nucleolus: the evolution of nucleolar compartmentsMarc Thiry
Laboratory of Cell and Tissue Biology, Department of Life Sciences, Faculty of Sciences, Universite de Liege, Liege, Belgium
Trends Cell Biol 15:194-9. 2005..We believe that these conclusions have important implications for understanding the structure-function relationships within this key cellular organelle...
- c-Myc associates with ribosomal DNA and activates RNA polymerase I transcriptionAzadeh Arabi
Södertörns högskola, S 141 89 Huddinge, Sweden
Nat Cell Biol 7:303-10. 2005..These results suggest that c-Myc coordinates the activity of all three nuclear RNA polymerases, and thereby plays a key role in regulating ribosome biogenesis and cell growth...
- RNA content in the nucleolus alters p53 acetylation via MYBBP1ATakao Kuroda
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
EMBO J 30:1054-66. 2011..Our observations indicated that a dynamic equilibrium between RNA generation and export regulated nucleolar RNA content. Perturbation of this balance by nucleolar stress altered the nucleolar RNA content and modulated p53 activity...
- Chromosome arm length and nuclear constraints determine the dynamic relationship of yeast subtelomeresPierre Therizols
Unité de Génétique Moléculaire des Levures, Centre National de la Recherche Scientifique, Institut Pasteur, Université Pierre et Marie Curie 75724 Paris, France
Proc Natl Acad Sci U S A 107:2025-30. 2010..Our results suggest that interactions between subtelomeres are nonspecific and instead governed by physical constraints, including chromosome structure, attachment to the SPB, and nuclear crowding...
- The nucleolus: structure/function relationship in RNA metabolismDanièle Hernandez-Verdun
Nuclei and Cell Cycle, Institut Jacques Monod UMR 7592 CNRS, Universite Paris Diderot, 75205 Paris Cedex 13, France
Wiley Interdiscip Rev RNA 1:415-31. 2010..The last part of this review presents recent advances in nucleolar dysfunctions in human pathology such as cancer and virus infections that modify the nucleolar organization...
- Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexesRebecca A Haeusler
Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA
Genes Dev 22:2204-14. 2008..These observations suggest a widespread role for condensin in gene organization and packaging of the interphase yeast nucleus...
- Formation of nuclear heterochromatin: the nucleolar point of viewClaudio Guetg
Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Zurich, Switzerland
Epigenetics 7:811-4. 2012....
- Emerging roles of the neuronal nucleolusMichal Hetman
Kentucky Spinal Cord Injury Research Center and Department of Neurological Surgery, University of Louisville, Louisville, Kentucky 40292, USA
Trends Neurosci 35:305-14. 2012..Hence, disrupted neurodevelopment or neurodegeneration are among the likely consequences of nucleolar dysfunction. Conversely, the presence of active nucleoli may determine the potential for neurorepair...
- Signal recognition particle components in the nucleolusJ C Politz
Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 97:55-60. 2000..Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves...
- Chemotherapeutic drugs inhibit ribosome biogenesis at various levelsKaspar Burger
Institute for Clinical Molecular Biology and Tumor Genetics, Center for Integrated Protein Science Munich CIPSM, Germany
J Biol Chem 285:12416-25. 2010..Flavopiridol and 5-fluorouracil showed a strong synergism for inhibition of rRNA processing. We conclude that inhibition of ribosome biogenesis by chemotherapeutic drugs potentially may contribute to the efficacy of therapeutic regimens...
- Nucleolar size indicates the rapidity of cell proliferation in cancer tissuesM Derenzini
Dipartimento di Patologia Sperimentale, Universita di Bologna, Italy
J Pathol 191:181-6. 2000..Evaluation of nucleolar size after silver staining of AgNOR proteins represents a unique parameter for the histological assessment of rapidity of cell proliferation in tumour lesions...
- The nucleolus directly regulates p53 export and degradationMark T Boyd
p53 MDM2 Research Group, Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool L69 3GA, England, UK
J Cell Biol 194:689-703. 2011..In addition, our results indicate that p53 is exported by two pathways: one stress sensitive and one stress insensitive, the latter being regulated by activities present in the nucleolus...
- Nucleolar proteome dynamicsJens S Andersen
Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, Denmark
Nature 433:77-83. 2005....
- Nucleolar targeting: the hub of the matterEdward Emmott
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, England, UK
EMBO Rep 10:231-8. 2009..In parallel to other subnuclear structures, such as PML bodies, the proteins that are involved in the formation and maintenance of the nucleolus are inexorably linked to nucleolar trafficking...
- PML regulates p53 stability by sequestering Mdm2 to the nucleolusRosa Bernardi
Cancer Biology and Genetics Program, Department of Pathology and Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Nat Cell Biol 6:665-72. 2004..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression...
- Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiaeChristophe Dez
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
EMBO J 25:1534-46. 2006..Localization of pre-ribosomes to this focus was lost in sda1-2 strains lacking Trf4p or Rrp6p. We designate this nucleolar focus the No-body and propose that it represents a site of pre-ribosome surveillance...
- Accurate processing of a eukaryotic precursor ribosomal RNA by ribonuclease MRP in vitroZ Lygerou
European Molecular Biology Laboratory, Heidelberg, Germany
Science 272:268-70. 1996..Biochemical purification and the use of extracts depleted of the MRP RNA demonstrate that endonucleolytic cleavage of the pre-rRNA is directly mediated by RNase MRP. This establishes a role for RNase MRP in the nucleolus...
- Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunitsMarie Françoise O'Donohue
Laboratoire de Biologie Moléculaire des Eucaryotes, Université de Toulouse UPS and Centre National de La Recherche Scientifique, F 31000 Toulouse, France
J Cell Biol 190:853-66. 2010..Interestingly, the functional dichotomy of RPS proteins reported in this study is correlated with the mutation frequency of RPS genes in Diamond-Blackfan anemia...
- The multifunctional nucleolusFrancois Michel Boisvert
Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
Nat Rev Mol Cell Biol 8:574-85. 2007....
- MicroRNAs with a nucleolar locationJoan C Ritland Politz
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
RNA 15:1705-15. 2009....
- The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaksMichael Kruhlak
Experimental Immunology, NCI
Nature 447:730-4. 2007..Our data reveal a novel ATM/NBS1/MDC1-dependent pathway that shuts down ribosomal gene transcription in response to chromosome breaks...
- A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesisFrançois Dragon
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520 8040, USA
Nature 417:967-70. 2002..We have termed this large RNP the small subunit (SSU) processome...
- Nucleolus: the fascinating nuclear bodyValentina Sirri
Nuclei and Cell Cycle, CNRS, Universite Paris VI, Universite Paris VII, Institut Jacques Monod, 2 place Jussieu, 75251, Paris Cedex 05, France
Histochem Cell Biol 129:13-31. 2008..Perspectives on the evolution of this research area are proposed...
- Assembly and disassembly of the nucleolus during the cell cycleDanièle Hernandez-Verdun
Universite Paris Diderot, France
Nucleus 2:189-94. 2011..The dynamics of assembly/disassembly of the nucleolus is time and space regulated...
- Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosisXuejun Yuan
Division of Molecular Biology of the Cell II, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
Mol Cell 19:77-87. 2005..The striking correlation between perturbation of nucleolar function, elevated levels of p53, and induction of cell suicide supports the view that the nucleolus is a stress sensor that regulates p53 activity...
- Rrs1 is involved in endoplasmic reticulum stress response in Huntington diseaseAlisia Carnemolla
Sector of Neurobiology, International School for Advanced Studies, Via Beirut 2 4, 34151 Trieste, Italy
J Biol Chem 284:18167-73. 2009..Interestingly, we demonstrate that ER stress is an early event in a presymptomatic HD mouse model that persists throughout the life span of the rodent. We further show that ER stress also occurs in postmortem brains of HD patients...
- MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cellsJoan C Ritland Politz
Department of Biochemistry and Molecular Pharmacology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 103:18957-62. 2006..These results suggest that miR-206 may associate both with nascent ribosomes in the nucleolus and with exported, functional ribosomes in the cytoplasm...
- Nucleolin: a multiFACeTed proteinFabien Mongelard
Laboratoire Joliot Curie, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69007 Lyon, France
Trends Cell Biol 17:80-6. 2007..These new properties could help reconcile discrepancies in the literature regarding the role of nucleolin in transcription...
- High-resolution whole-genome sequencing reveals that specific chromatin domains from most human chromosomes associate with nucleoliSilvana van Koningsbruggen
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
Mol Biol Cell 21:3735-48. 2010..Unexpectedly, both the direct DNA sequencing and fluorescence photoactivation data show that certain chromatin loci can specifically associate with either the nucleolus, or the nuclear envelope...
- Initial genomics of the human nucleolusAttila Nemeth
Department of Biochemistry III, University of Regensburg, Regensburg, Germany
PLoS Genet 6:e1000889. 2010..Altogether, our findings describe the nature of DNA sequences associated with the human nucleolus and provide insights into the function of the nucleolus in genome organization and establishment of nuclear architecture...
- Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription systemOlivier Gadal
Laboratoire de Physio Génomique, Service de Biochimie et Génétique Moléculaire, CEA Saclay, Batiment 142, F 91191 Gif sur Yvette, France
EMBO J 21:5498-507. 2002..Conversely, rpa34-Delta mutants lacking Rpa34, a non-essential subunit associated with Rpa49, are lethal in top1-Delta but not in top3-Delta...
- Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolusS Llanos
Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
Nat Cell Biol 3:445-52. 2001..We therefore propose that nucleolar localization is not essential for ARF function but may enhance the availability of ARF to inhibit MDM2...
- Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibitionVittoria Matafora
Division of Genomics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy
Mol Cell Proteomics 8:2243-55. 2009..The analysis of the nature of the SUMO-1 targets identified in this study strongly indicates that sumoylation, acting in coordination with the ubiquitin-proteasome system, regulates the maintenance of nucleolar integrity...
- SUMO-1 modification alters ADAR1 editing activityJoana M P Desterro
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
Mol Biol Cell 16:5115-26. 2005..Moreover, modification of wild-type recombinant ADAR1 by SUMO-1 reduces the editing activity of the enzyme in vitro. Taken together these data suggest a novel role for sumoylation in regulating RNA-editing activity...
- Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cellsMohammed Abdullahel Amin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
Biochem J 415:345-51. 2008..These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells...
- The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functionsA G Balakin
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA
Cell 86:823-34. 1996..Binding of this or another common small nucleolar ribonucleoprotein particle protein is predicted to be a critical entry point to snoRNA posttranscriptional life, including precise formation of the snoRNA 3' end...
- Tryptophans 286 and 288 in the C-terminal region of protein B23.1 are important for its nucleolar localizationYuki Nishimura
Department of Sustainable Resource Science, Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514 8507, Japan
Biosci Biotechnol Biochem 66:2239-42. 2002..1 mutant proteins fused with green fluorescent protein in HeLa cells. The results suggested that the two C-terminal tryptophan residues (Trp-286 and Trp-288) of protein B23.1 were important in this phenomenon...
- An intranucleolar body associated with rDNASaskia Hutten
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK
Chromosoma 120:481-99. 2011..INBs are abundant in nucleoli of unstressed cells during S phase and localise in close proximity to rDNA with heterochromatic features. The data suggest the INB is linked with regulation of rDNA transcription and/or maintenance of rDNA...
- Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availabilityL Latonen
Molecular Cancer Biology Program and Haartman Institute, University of Helsinki, Helsinki, Finland
Oncogene 30:790-805. 2011..These findings show that the nucleolus controls protein and RNA surveillance and export by the ubiquitin pathway in a previously unidentified manner, and provide mechanistic insight into the cellular effects of PIs...
- Dynamic proteomics of individual cancer cells in response to a drugA A Cohen
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Science 322:1511-6. 2008..We identify proteins whose dynamics differ widely between cells, in a way that corresponds to the outcomes-cell death or survival. This opens the way to understanding molecular responses to drugs in individual cells...
- Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cellsHui Gao
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Biol Cell 14:4826-34. 2003..Together these data support a role for Mus81 in recombination repair in higher eukaryotes...
- Ribosome assembly in eukaryotesMicheline Fromont-Racine
Genetique des Interactions Macromoleculaires, Institut Pasteur, 75724 Cedex 15, Paris, France
Gene 313:17-42. 2003..Proteomic approaches have provided the first indications for a ribosome assembly pathway in eukaryotes and confirmed the dynamic character of the whole process...
- Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteinsS Pinol-Roma
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208
Genes Dev 2:215-27. 1988..We describe a general method for the large-scale purification of hnRNP proteins by affinity chromatography on ssDNA columns and its use for the production of new monoclonal antibodies to hnRNP proteins...
- Characterization of AtNUC-L1 reveals a central role of nucleolin in nucleolus organization and silencing of AtNUC-L2 gene in ArabidopsisFrederic Pontvianne
Unité Mixte de Recherche Centre National de la Recherche Scientifique 5096, Universite de Perpignan, Perpignan, 66860 Perpignan, France
Mol Biol Cell 18:369-79. 2007..This work is the first description of a higher eukaryotic organism with a disrupted nucleolin-like gene and defines a new role for nucleolin in nucleolus structure and rDNA chromatin organization...
- Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNPNicholas J Watkins
Max Planck Institut fur biophysikalische Chemie, Abteilung Zelluläre Biochemie, D 37070, Gottingen, Germany
Mol Cell Biol 22:8342-52. 2002..5K, to the snoRNA. Indeed, the sequence of stem II was essential for nucleolar localization of U14 snoRNA microinjected into HeLa cells. Thus, the conserved sequence of stem II determines the specific assembly of the box C/D snoRNP...
- The role of Cdc55 in the spindle checkpoint is through regulation of mitotic exit in Saccharomyces cerevisiaeChristopher M Yellman
Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, University of Virginia, Charlottesville, VA 22908, USA
Mol Biol Cell 17:658-66. 2006..Interestingly, the B-type cyclin Clb2 is partially stable during premature activation of mitotic exit in a cdc55 mutant, indicating mitotic exit is incomplete...
- Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesisZ Strezoska
Department of Molecular Genetics, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60607 7170, USA
Mol Cell Biol 20:5516-28. 2000..These studies thus identify Bop1 as a novel, nonribosomal mammalian protein that plays a key role in the formation of the mature 28S and 5.8S rRNAs and in the biogenesis of the 60S ribosomal subunit...
- Nucleolar assembly of the rRNA processing machinery in living cellsT M Savino
Institut Jacques Monod, UMR 7592, 2 place Jussieu, 75251 Paris Cedex 05, France
J Cell Biol 153:1097-110. 2001..This demonstrates the formation of one functional domain by cooperative interactions between different chromosome territories...
- Nucleolar proteomics and viral infectionJulian A Hiscox
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
Proteomics 10:4077-86. 2010....
- In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolusTao Wei
Department of Cell Biology, School of Life Sciences, Peking University, 100871, China
J Cell Sci 116:1117-25. 2003..The DNA clumps within the fibrillar center were probably the anchoring sites for rDNA arrangement...
- Immobilization of proteins in the nucleolus by ribosomal intergenic spacer noncoding RNATimothy E Audas
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada
Mol Cell 45:147-57. 2012..We propose a model whereby protein immobilization by ncRNA is a posttranslational regulatory mechanism...
- NOF1 encodes an Arabidopsis protein involved in the control of rRNA expressionErwana Harscoët
INRA, IJPB, UMR 1318 INRA AgroParisTech, Versailles, France
PLoS ONE 5:e12829. 2010....
- Proinsulin C-peptide regulates ribosomal RNA expressionEmma Lindahl
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
J Biol Chem 285:3462-9. 2010..This regulation of ribosomal RNA provides a mechanism by which C-peptide can exert transcriptional effects and implies that the peptide has growth factor activity...
- Proteomic profiling of the human T-cell nucleolusMohamed Ali Jarboui
Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin, Belfield, Dublin 4, Ireland
Mol Immunol 49:441-52. 2011....
- Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiaeDaisuke Morita
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama 1 4 4, Higashi Hiroshima 739 8528, Japan
J Biol Chem 277:28780-6. 2002..An analysis of processing intermediates by primer extension shows that the Rpf2p depletion leads to an accumulation of 27 SB pre-rRNA, suggesting that Rpf2p is required for the processing of 27 SB into 25 S rRNA...
- Tagging of functional ribosomes in living cells by HaloTag® technologySimone Gallo
San Raffaele Scientific Institute DIBIT, Via Olgettina 58, 20132, Milan, Italy
In Vitro Cell Dev Biol Anim 47:132-8. 2011..In addition, the level of overexpression and degradation may vary upon different constructs. In summary, HaloTag® technology is highly suitable to ribosome tagging, but requires prior characterization for each construct...
- Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolusSuisheng Zhang
Department of Biochemistry, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D 07708 Jena, Germany
Exp Cell Res 293:248-58. 2004..Taken together, these results support the idea that an actin-based filamentous network may anchor NDH II at the nucleolar periphery for pre-ribosomal RNA processing, ribosome assembly, and/or transport...
- NoD: a Nucleolar localization sequence detector for eukaryotic and viral proteinsMichelle S Scott
Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
BMC Bioinformatics 12:317. 2011..Although many proteins have been reported to contain NoLSs, the systematic characterization of this group of targeting motifs has only recently been carried out...
- Novel variants of muscle calpain 3 identified in human melanoma cells: cisplatin-induced changes in vitro and differential expression in melanocytic lesionsD Moretti
Department of Physiopathology, Experimental Medicine and Public Health, University of Siena, Italy
Carcinogenesis 30:960-7. 2009..On the whole, our observations suggest that calpain 3 variants can play a proapoptotic role in melanoma cells and its downregulation, as observed in highly aggressive lesions, could contribute to melanoma progression...
- Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteinsTim Krüger
Department of Cell and Developmental Biology, Biocenter, University of Wurzburg, Wurzburg, Germany
J Cell Biol 177:573-8. 2007....
- Discrete foci containing RNase A are found in nucleoli of HeLa cells after aging in cultureM Costanzo
Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Universita di Pavia, Italy
Eur J Histochem 55:e15. 2011..These bodies also contain RNA and lack ribosomal S3 proteins, and may represent either simple storage sites or areas where RNA degradation takes place...
- Involvement of the plant nucleolus in virus and viroid infections: parallels with animal pathosystemsM E Taliansky
Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom
Adv Virus Res 77:119-58. 2010..Investigating the interactions between plant viruses and the nucleolus will facilitate the design of novel strategies to control plant virus infections...
- Silencing of ribosomal protein S9 elicits a multitude of cellular responses inhibiting the growth of cancer cells subsequent to p53 activationMikael S Lindström
Department of Oncology Pathology, Karolinska Institutet, Stockholm, Sweden
PLoS ONE 5:e9578. 2010..The effects of ribosomal protein loss in cultured mammalian cells have not been thoroughly investigated. Here we characterize the cellular stress response caused by depletion of ribosomal protein S9 (RPS9)...
- Systematic identification of pathways that couple cell growth and division in yeastPaul Jorgensen
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
Science 297:395-400. 2002..Cell growth and division appear to be coupled by multiple conserved mechanisms...
- Nucleolar disruption in dopaminergic neurons leads to oxidative damage and parkinsonism through repression of mammalian target of rapamycin signalingClaus Rieker
Division of Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg, Germany
J Neurosci 31:453-60. 2011..Collectively, these observations suggest that the interplay between nucleolar dysfunction and increased oxidative stress, involving p53 and mTOR signaling, may constitute a destructive axis in experimental and sporadic PD...
- Identification of a cryptic nucleolar-localization signal in MDM2M A Lohrum
NCI FCRDC, Building 560, Room 22 96, West 7th Street, Frederick, Maryland 21702 1201, USA
Nat Cell Biol 2:179-81. 2000
- Perturbation of 60 S ribosomal biogenesis results in ribosomal protein L5- and L11-dependent p53 activationXiao Xin Sun
Department of Molecular and Medical Genetics, School of Medicine, Oregon Health and Science University, Portland, Oregon 97239, USA
J Biol Chem 285:25812-21. 2010..These results demonstrate that NEDDylation of L11 plays a critical role in mediating p53 activation in response to perturbation of ribosomal biogenesis...
- Expression and localization of Werner syndrome protein is modulated by SIRT1 and PMLRasa Vaitiekunaite
Department of Tumor Biology, Maria Skłodowska Curie Memorial Cancer Center, ul Wybrzeze Armii Krajowej 15, 44 101 Gliwice, Poland
Mech Ageing Dev 128:650-61. 2007..Our data indicate for the first time that SIRT1 protein may be functionally associated with WRN and BLM helicases and that some major SIRT1 functions may not require its deacetylase activity...
- Autoantibody profiles in systemic sclerosis: predictive value for clinical evaluation and prognosisYasuhito Hamaguchi
Department of Dermatology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920 8641, Japan
J Dermatol 37:42-53. 2010....
- Alterations in nucleolar structure and gene expression programs in prostatic neoplasia are driven by the MYC oncogeneCheryl M Koh
Department of Pathology, The Johns Hopkins University, School of Medicine, Baltimore, MD 21231, USA
Am J Pathol 178:1824-34. 2011....
- Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc+ AMLBrunangelo Falini
Institute of Hematology, University of Perugia, 06122 Perugia, Italy
Blood 107:4514-23. 2006..These findings indicate that potential therapeutic strategies aimed to retarget NPM to its physiological sites will have to overcome 2 obstacles, the new NES motif and the mutated tryptophan(s) at the NPM mutant C-terminus...
- Regulation of nucleolar signalling to p53 through NEDDylation of L11Anders Sundqvist
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK
EMBO Rep 10:1132-9. 2009..By controlling the correct localization and stability of L11, NEDD8 acts as a crucial, new regulator of nucleolar signalling to p53...
- A nucleolar targeting signal in PML-I addresses PML to nucleolar caps in stressed or senescent cellsWilfried Condemine
CNRS Université de Paris 7 UMR7151, Equipe Labellisee par la Ligue Contre le Cancer, Hopital St Louis, 1 Av C Vellefaux 75475, Paris Cedex 10, France
J Cell Sci 120:3219-27. 2007..Later, poly-ubiquitin conjugates are found on the outer shell or within most of these senescence-associated PML bodies. Thus, unexpectedly, the scarcely studied PML-I isoform links PML bodies, nucleolus, senescence and proteolysis...
- Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxiaO A Koroleva
Department of Cell Biology, John Ines Centre, Colney, Norwich NR4 7UH, United Kingdom
Plant Cell 21:1592-606. 2009..Sequestration of eIF4A-III into nuclear pools with different mobility is likely to reflect the transcriptional and mRNA processing state of the cell...
- NORMAL AND MUTANT CHORION GENES OF DROSOPHILAAllan Spradling; Fiscal Year: 2003..Several candidate genes that are known to be necessary for nurse cell nucleolus formation, or for normal nurse cell chromosome structure, will be tested...
- CANCER RESEARCH PROGRAMHarris Busch; Fiscal Year: 1991....
- A Novel HTP Assay for Identification of Compounds that Reduce PNC PrevalenceSui Huang; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Survival signaling in CNS neurons exposed to DNA damageMichal Hetman; Fiscal Year: 2008..The proposed research may result in identification of new drug targets for treatment of neurological diseases and/or deleterious neurological side effects of cancer therapy. ..
- Spondyloarthritis 2006; The Unmet NeedsJohn Reveille; Fiscal Year: 2006..unreadable] [unreadable] [unreadable] [unreadable]..
- RNA Transport Within the NucleusThoru Pederson; Fiscal Year: 2005..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
- The Perinucleolar Compartment as Breast Cancer MarkerSui Huang; Fiscal Year: 2005..We expect the proposed studies will clarify whether the PNC, an easily detectable cellular structure, could improve the accuracy of breast cancer prognosis and prediction when used in combination with existing markers. ..
- Biogenesis of Eukaryotic RibosomesSUSAN GERBI; Fiscal Year: 2004..The proposed studies should elucidate the importance of dynamic RNA-RNA interactions during ribosome biogenesis, a fundamental process essential for cell growth and viability. ..
- PERINUCLEOLAR COMPARTMENT AND CANCERSui Huang; Fiscal Year: 2003..The results of these experiments will provide an important foundation to address the significance of the PNC in malignant cells. ..
- AUTOANTIGEN RNA HELICASE II/GUBenigno Valdez; Fiscal Year: 2003..This study may also provide an impetus for understanding the pathological roles of RH-II/Gu on the elicitation of autoimmune responses. ..
- RNA PROCESSING AND RIBONUCLEOPROTEINThoru Pederson; Fiscal Year: 2001..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
- Gene Amplification-Sciarid DNA PuffsSUSAN GERBI; Fiscal Year: 2006..Information gained from the unique Sciara system should behelpful in understanding the mechanisms used by metazoa to specify an origin and to regulate its activation. ..
- FAMILY STUDIES OF THE GENETICS OF ANKYLOSING SPONDYLITISJohn Reveille; Fiscal Year: 2003..abstract_text> ..
- Stem-like Cancer Cells in Breast TumorigenesisROBERT TSAI; Fiscal Year: 2009..abstract_text> ..
- Prion-Based Mechanisms in Metazoan DevelopmentKORIE HANDWERGER; Fiscal Year: 2006..Finally, CePrDps will be examined for their ability to regulate development in the absence of their putative prion domains. ..
- Insulin and Oocyte Chromatin SegregationGary Smith; Fiscal Year: 2009..abstract_text> ..
- Molecular basis of neuronal dysfunction in AIDSFrancesca Peruzzi; Fiscal Year: 2008..The outcome of these studies will provide a new information on the pathways which are affected by HIV-1 Tat, and will assist to improve our effort for developing strategies against HIV-1 induced neuronal cell injury. ..
- Biochemical Genomics Linking Genes and ActivitiesEric Phizicky; Fiscal Year: 2004..Application of these techniques to other organisms, including humans and pathogens, will greatly accelerate biochemical analysis and can be used to rapidly identify drug targets. ..
- Cloning and analysis of centrosome-associated RNAMARK ALLIEGRO; Fiscal Year: 2007..If our hypothesis is correct, this study will reopen a line of inquiry with impact in areas of cell and developmental biology ranging from control of cell division to evolution of eukaryotes. [unreadable] [unreadable]..
- INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLSMichael Berns; Fiscal Year: 2001..The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research. ..
- Retrotransposons as site-specific gene delivery vectorsJOHN GOODIER; Fiscal Year: 2003..In addition, I will assay in cell culture the efficiency of the L1 at integrating increasing lengths of exogenous DNA. ..
- Telomerase Transport and TargetingMICHAEL TERNS; Fiscal Year: 2008..abstract_text> ..
- Role of RecQ Helicases to Prevent Senescence By c-MycCarla Grandori; Fiscal Year: 2010..If so, drug inhibitors of WRN function could be proposed for the treatment of cancer. ..
- Preventive of Bile Acid-Induced Colonic Oxidative DNA DamageClaire Margaret Payne; Fiscal Year: 2010..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
- Cytochrome C Oxidase I:A Biomarker for Colon Cancer RiskClaire Payne; Fiscal Year: 2006..Various molecular and cellular techniques will be used in these studies. ..
- LASER PHOTORADIATION THERAPY OF MALIGNANT TUMORSMichael Berns; Fiscal Year: 2002..Response will be evaluated at 3 month intervals for up to one year post treatment. ..
- BIOREGULATION OF THE SPERMATOGONIAL STEM CELL LINEAGELAURA TRES; Fiscal Year: 2003..2. In the long range, the discovery of ways to prevent and modulate spermatogonial cell death may have significant therapeutic and anticonceptive potentials in humans. ..
- INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLSMichael Berns; Fiscal Year: 2003..The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research. ..
- Recombination and meiotic progression in the mouseLaura Reinholdt; Fiscal Year: 2004..Finally, a new, potentially meiotic mutation, Mei5, which may cause a meiotic defect that escapes detection by meiotic checkpoint mechanisms, will be phenotypically characterized and genetically mapped. ..
- MHC-Defined and Identical Twin Monkeys for ResearchJoseph Kemnitz; Fiscal Year: 2005..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
- Human Posterior Cricoarytenoid Muscle Aging MechanismsLeslie Malmgren; Fiscal Year: 2005..Since processes such as apoptosis are potential targets for clinical intervention, the proposed studies will be essential to the design of improved strategies for the prevention or treatment of age-related laryngeal dysfunction. ..
- PROTEIN PHOSPHATASES AND OOCYTE MEIOSISGary Smith; Fiscal Year: 2002..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
- DIRECT TARGET GENES OF THE MYC ONCOPROTEINCarla Grandori; Fiscal Year: 2002..These studies will provide several pieces of the puzzle necessary to understand how Myc functions as an oncogene. ..
- Import of RNA into MitochondriaMARK SCHMITT; Fiscal Year: 2006..4) Identification of other RNA molecules imported into mitochondria. These experiments should provide a wealth of knowledge about this fundamental process. ..
- MITOSIS IN NORMAL AND NEOPLASTIC CELLSBILL BRINKLEY; Fiscal Year: 2006..abstract_text> ..
- DEVELOPMENTAL REGULATION OF GROWTH, SIZE AND SHAPENicholas E Baker; Fiscal Year: 2010....
- Novel Genes Expressed in Proliferating Endothelial CellsMARK ALLIEGRO; Fiscal Year: 2006..abstract_text> ..
- YEAST CHROMOSOME STRUCTURE, REPLICATION AND SEGREGATIONAngelika Amon; Fiscal Year: 2004..Our objective for this meeting is to continue the extremely high level of excitement and scientific excellence that has characterized the past seven conferences. ..
- Synapse-to-nucleus signaling components in PSDsBRYEN JORDAN; Fiscal Year: 2007..This work will increase our understanding of synaptic function which is critical in learning and memory. ..