cell nucleolus

Summary

Summary: Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)

Top Publications

  1. pmc The nucleolus under stress
    Séverine Boulon
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK
    Mol Cell 40:216-27. 2010
  2. ncbi Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
  3. ncbi Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype
    Brunangelo Falini
    Institute of Hematology, University of Perugia, Perugia, Italy
    N Engl J Med 352:254-66. 2005
  4. ncbi Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11
    Masato Sasaki
    Global Centers of Excellence Program, Akita University Graduate School of Medicine, Akita, Japan
    Nat Med 17:944-51. 2011
  5. ncbi Epigenetic control of rDNA loci in response to intracellular energy status
    Akiko Murayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8572, Japan
    Cell 133:627-39. 2008
  6. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
  7. pmc CAG expansion induces nucleolar stress in polyglutamine diseases
    Ho Tsoi
    Laboratory of Drosophila Research, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    Proc Natl Acad Sci U S A 109:13428-33. 2012
  8. ncbi Ribosome biogenesis surveillance: probing the ribosomal protein-Mdm2-p53 pathway
    C Deisenroth
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Oncogene 29:4253-60. 2010
  9. pmc Nucleolus, ribosomes, and cancer
    Lorenzo Montanaro
    Department of Experimental Pathology, University of Bologna, Bologna, Italy
    Am J Pathol 173:301-10. 2008
  10. pmc Poly(ADP-Ribose) polymerase 1 (PARP-1) regulates ribosomal biogenesis in Drosophila nucleoli
    Ernest K Boamah
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
    PLoS Genet 8:e1002442. 2012

Research Grants

  1. NORMAL AND MUTANT CHORION GENES OF DROSOPHILA
    Allan Spradling; Fiscal Year: 2003
  2. CANCER RESEARCH PROGRAM
    Harris Busch; Fiscal Year: 1991
  3. A Novel HTP Assay for Identification of Compounds that Reduce PNC Prevalence
    Sui Huang; Fiscal Year: 2008
  4. Survival signaling in CNS neurons exposed to DNA damage
    Michal Hetman; Fiscal Year: 2008
  5. Spondyloarthritis 2006; The Unmet Needs
    John Reveille; Fiscal Year: 2006
  6. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
  7. The Perinucleolar Compartment as Breast Cancer Marker
    Sui Huang; Fiscal Year: 2005
  8. Biogenesis of Eukaryotic Ribosomes
    SUSAN GERBI; Fiscal Year: 2004
  9. PERINUCLEOLAR COMPARTMENT AND CANCER
    Sui Huang; Fiscal Year: 2003
  10. AUTOANTIGEN RNA HELICASE II/GU
    Benigno Valdez; Fiscal Year: 2003

Detail Information

Publications276 found, 100 shown here

  1. pmc The nucleolus under stress
    Séverine Boulon
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, UK
    Mol Cell 40:216-27. 2010
    ..Crosstalk between nucleoli and CBs is also discussed in the context of stress responses...
  2. ncbi Driving ribosome assembly
    Dieter Kressler
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Biochim Biophys Acta 1803:673-83. 2010
    ..This review summarizes our current knowledge on eukaryotic ribosome assembly, with particular focus on the molecular role of the involved energy-consuming enzymes...
  3. ncbi Cytoplasmic nucleophosmin in acute myelogenous leukemia with a normal karyotype
    Brunangelo Falini
    Institute of Hematology, University of Perugia, Perugia, Italy
    N Engl J Med 352:254-66. 2005
    ..Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein...
  4. ncbi Regulation of the MDM2-P53 pathway and tumor growth by PICT1 via nucleolar RPL11
    Masato Sasaki
    Global Centers of Excellence Program, Akita University Graduate School of Medicine, Akita, Japan
    Nat Med 17:944-51. 2011
    ..When PICT1 is depleted in tumor cells with intact P53 signaling, the cells grow more slowly and accumulate P53. Thus, PICT1 is a potent regulator of the MDM2-P53 pathway and promotes tumor progression by retaining RPL11 in the nucleolus...
  5. ncbi Epigenetic control of rDNA loci in response to intracellular energy status
    Akiko Murayama
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8572, Japan
    Cell 133:627-39. 2008
    ..These findings provide key insight into the mechanisms of energy homeostasis in cells...
  6. pmc Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins
    Yun Wah Lam
    Division of Gene Regulation and Expression, Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Curr Biol 17:749-60. 2007
    ....
  7. pmc CAG expansion induces nucleolar stress in polyglutamine diseases
    Ho Tsoi
    Laboratory of Drosophila Research, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    Proc Natl Acad Sci U S A 109:13428-33. 2012
    ..Our work provides in vivo evidence that expanded CAG RNAs trigger nucleolar stress and induce apoptosis via p53 and describes a polyQ pathogenic mechanism that involves the nucleolus...
  8. ncbi Ribosome biogenesis surveillance: probing the ribosomal protein-Mdm2-p53 pathway
    C Deisenroth
    Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Oncogene 29:4253-60. 2010
    ..In this review, we will clarify how disruption to three major components of ribosome biogenesis can trigger nucleolar stress and activate p53, thereby lending support to a RP-Mdm2-p53 ribosome biogenesis surveillance pathway...
  9. pmc Nucleolus, ribosomes, and cancer
    Lorenzo Montanaro
    Department of Experimental Pathology, University of Bologna, Bologna, Italy
    Am J Pathol 173:301-10. 2008
    ....
  10. pmc Poly(ADP-Ribose) polymerase 1 (PARP-1) regulates ribosomal biogenesis in Drosophila nucleoli
    Ernest K Boamah
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States of America
    PLoS Genet 8:e1002442. 2012
    ..Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis...
  11. pmc Characterization and prediction of protein nucleolar localization sequences
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery and Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    Nucleic Acids Res 38:7388-99. 2010
    ..NoLSs are a prevalent type of targeting motif that is distinct from nuclear localization signals and that can be computationally predicted...
  12. pmc Nuclear neighborhoods and gene expression
    Rui Zhao
    Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY 11794, USA
    Curr Opin Genet Dev 19:172-9. 2009
    ..We are still at a very early, yet exciting stage in our journey toward deciphering the mechanism(s) that govern(s) the permissiveness of gene expression/repression within different nuclear neighborhoods...
  13. ncbi Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis
    Denis Drygin
    Cylene Pharmaceuticals, Inc, San Diego, CA 92121, USA
    Cancer Res 69:7653-61. 2009
    ..CX-3543 is the first G-quadruplex interactive agent to enter human clinical trials, and it is currently under evaluation against carcinoid/neuroendocrine tumors in a phase II clinical trial...
  14. ncbi Directed proteomic analysis of the human nucleolus
    Jens S Andersen
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark
    Curr Biol 12:1-11. 2002
    ..Recent studies suggest it may also have roles in RNA transport, RNA modification, and cell cycle regulation. Despite over 150 years of research into nucleoli, many aspects of their structure and function remain uncharacterized...
  15. pmc Nucleophosmin (B23) targets ARF to nucleoli and inhibits its function
    Chandrashekhar Korgaonkar
    Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City, IA 52242, USA
    Mol Cell Biol 25:1258-71. 2005
    ..Conversely, NPM overexpression antagonized ARF function while increasing its nucleolar localization. These data suggest that NPM inhibits ARF's p53-dependent activity by targeting it to nucleoli and impairing ARF-Mdm2 association...
  16. ncbi CSB is a component of RNA pol I transcription
    John Bradsher
    Institut de Genetique et de Biologie Moleculaire et Cellulaire CNRS INSERM ULP, F 67404, Illkirch Cedex, France
    Mol Cell 10:819-29. 2002
    ..In addition to revealing an unanticipated function for CSB in rRNA synthesis, we show that the fragility of this complex could be one factor contributing to the CS phenotype...
  17. ncbi Small nucleolar RNAs: an abundant group of noncoding RNAs with diverse cellular functions
    Tamas Kiss
    Laboratoire de Biologie Moleculaire Eucaryote du CNRS, Universite Paul Sabatier, 118 route de Narbonne, 31062 Cedex, Toulouse, France
    Cell 109:145-8. 2002
    ....
  18. pmc Biogenesis of the signal recognition particle (SRP) involves import of SRP proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated export
    H Grosshans
    Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 153:745-62. 2001
    ..This particle can then be targeted to the nuclear pores and is subsequently exported to the cytoplasm in an Xpo1p-dependent way...
  19. pmc RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
    Benjamin Albert
    Laboratoire de Biologie Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Universite de Toulouse, F 31000 Toulouse, France
    J Cell Biol 192:277-93. 2011
    ..Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly...
  20. ncbi Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complex
    Nicholas J Watkins
    Max Planck Institute of Biophysical Chemistry, Am Fassberg 11, D 37070 Gottingen, Germany
    Mol Cell 16:789-98. 2004
    ..We believe that the assembly complex coordinates snoRNA processing, snoRNP assembly, restructuring, and localization...
  21. pmc The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during development
    Alexei Tulin
    Howard Hughes Medical Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Genes Dev 16:2108-19. 2002
    ..We propose that PARP-e autoregulates Parp transcription by influencing the chromatin structure of its heterochromatic environment. Our results indicate that Parp plays a fundamental role organizing the structure of Drosophila chromatin...
  22. ncbi Nucleolar protein NPM interacts with HDM2 and protects tumor suppressor protein p53 from HDM2-mediated degradation
    Sari Kurki
    Haartman Institute and Molecular Cancer Biology Research Program, Biomedicum Helsinki, University of Helsinki, P O Box 63, FIN 00014 Helsinki, Helsinki, Finland
    Cancer Cell 5:465-75. 2004
    ..The results demonstrate novel associations of HDM2 and K cyclin with NPM and implicate NPM as a crucial controller of p53 through inhibition of HDM2...
  23. pmc Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition
    Yaron Shav-Tal
    Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100 Israel
    Mol Biol Cell 16:2395-413. 2005
    ....
  24. pmc Modulation of RNA editing by functional nucleolar sequestration of ADAR2
    Christopher L Sansam
    Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA
    Proc Natl Acad Sci U S A 100:14018-23. 2003
    ..These observations indicate that the nucleolar localization of ADAR2 represents an important mechanism by which RNA editing can be modulated by the sequestration of enzymatic activity from potential RNA substrates in the nucleoplasm...
  25. pmc Inhibition of homologous recombination by a cohesin-associated clamp complex recruited to the rDNA recombination enhancer
    Julie Huang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 20:2887-901. 2006
    ....
  26. pmc Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere
    Lee H Wong
    Chromosome and Chromatin Research Laboratory, Murdoch Children s Research Institute and Department of Paediatrics, University of Melbourne, Royal Children s Hospital, Parkville, Victoria, Australia
    Genome Res 17:1146-60. 2007
    ....
  27. ncbi Intergenic transcripts regulate the epigenetic state of rRNA genes
    Christine Mayer
    German Cancer Research Center, Division of Molecular Biology of the Cell II, D 69120 Heidelberg, Germany
    Mol Cell 22:351-61. 2006
    ..The results reveal an important contribution of processed IGS transcripts in chromatin structure and epigenetic control of the rDNA locus...
  28. ncbi Birth of a nucleolus: the evolution of nucleolar compartments
    Marc Thiry
    Laboratory of Cell and Tissue Biology, Department of Life Sciences, Faculty of Sciences, Universite de Liege, Liege, Belgium
    Trends Cell Biol 15:194-9. 2005
    ..We believe that these conclusions have important implications for understanding the structure-function relationships within this key cellular organelle...
  29. ncbi c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription
    Azadeh Arabi
    Södertörns högskola, S 141 89 Huddinge, Sweden
    Nat Cell Biol 7:303-10. 2005
    ..These results suggest that c-Myc coordinates the activity of all three nuclear RNA polymerases, and thereby plays a key role in regulating ribosome biogenesis and cell growth...
  30. pmc RNA content in the nucleolus alters p53 acetylation via MYBBP1A
    Takao Kuroda
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    EMBO J 30:1054-66. 2011
    ..Our observations indicated that a dynamic equilibrium between RNA generation and export regulated nucleolar RNA content. Perturbation of this balance by nucleolar stress altered the nucleolar RNA content and modulated p53 activity...
  31. pmc Chromosome arm length and nuclear constraints determine the dynamic relationship of yeast subtelomeres
    Pierre Therizols
    Unité de Génétique Moléculaire des Levures, Centre National de la Recherche Scientifique, Institut Pasteur, Université Pierre et Marie Curie 75724 Paris, France
    Proc Natl Acad Sci U S A 107:2025-30. 2010
    ..Our results suggest that interactions between subtelomeres are nonspecific and instead governed by physical constraints, including chromosome structure, attachment to the SPB, and nuclear crowding...
  32. ncbi The nucleolus: structure/function relationship in RNA metabolism
    Danièle Hernandez-Verdun
    Nuclei and Cell Cycle, Institut Jacques Monod UMR 7592 CNRS, Universite Paris Diderot, 75205 Paris Cedex 13, France
    Wiley Interdiscip Rev RNA 1:415-31. 2010
    ..The last part of this review presents recent advances in nucleolar dysfunctions in human pathology such as cancer and virus infections that modify the nucleolar organization...
  33. pmc Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes
    Rebecca A Haeusler
    Department of Biological Chemistry, The University of Michigan, Ann Arbor, MI 48109, USA
    Genes Dev 22:2204-14. 2008
    ..These observations suggest a widespread role for condensin in gene organization and packaging of the interphase yeast nucleus...
  34. pmc Formation of nuclear heterochromatin: the nucleolar point of view
    Claudio Guetg
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Zurich, Switzerland
    Epigenetics 7:811-4. 2012
    ....
  35. pmc Emerging roles of the neuronal nucleolus
    Michal Hetman
    Kentucky Spinal Cord Injury Research Center and Department of Neurological Surgery, University of Louisville, Louisville, Kentucky 40292, USA
    Trends Neurosci 35:305-14. 2012
    ..Hence, disrupted neurodevelopment or neurodegeneration are among the likely consequences of nucleolar dysfunction. Conversely, the presence of active nucleoli may determine the potential for neurorepair...
  36. pmc Signal recognition particle components in the nucleolus
    J C Politz
    Department of Biochemistry, University of Massachusetts Medical School, 377 Plantation Street, Suite 337, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 97:55-60. 2000
    ..Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves...
  37. pmc Chemotherapeutic drugs inhibit ribosome biogenesis at various levels
    Kaspar Burger
    Institute for Clinical Molecular Biology and Tumor Genetics, Center for Integrated Protein Science Munich CIPSM, Germany
    J Biol Chem 285:12416-25. 2010
    ..Flavopiridol and 5-fluorouracil showed a strong synergism for inhibition of rRNA processing. We conclude that inhibition of ribosome biogenesis by chemotherapeutic drugs potentially may contribute to the efficacy of therapeutic regimens...
  38. ncbi Nucleolar size indicates the rapidity of cell proliferation in cancer tissues
    M Derenzini
    Dipartimento di Patologia Sperimentale, Universita di Bologna, Italy
    J Pathol 191:181-6. 2000
    ..Evaluation of nucleolar size after silver staining of AgNOR proteins represents a unique parameter for the histological assessment of rapidity of cell proliferation in tumour lesions...
  39. pmc The nucleolus directly regulates p53 export and degradation
    Mark T Boyd
    p53 MDM2 Research Group, Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool L69 3GA, England, UK
    J Cell Biol 194:689-703. 2011
    ..In addition, our results indicate that p53 is exported by two pathways: one stress sensitive and one stress insensitive, the latter being regulated by activities present in the nucleolus...
  40. ncbi Nucleolar proteome dynamics
    Jens S Andersen
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, Denmark
    Nature 433:77-83. 2005
    ....
  41. pmc Nucleolar targeting: the hub of the matter
    Edward Emmott
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, England, UK
    EMBO Rep 10:231-8. 2009
    ..In parallel to other subnuclear structures, such as PML bodies, the proteins that are involved in the formation and maintenance of the nucleolus are inexorably linked to nucleolar trafficking...
  42. ncbi PML regulates p53 stability by sequestering Mdm2 to the nucleolus
    Rosa Bernardi
    Cancer Biology and Genetics Program, Department of Pathology and Medicine, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Nat Cell Biol 6:665-72. 2004
    ..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression...
  43. pmc Surveillance of nuclear-restricted pre-ribosomes within a subnucleolar region of Saccharomyces cerevisiae
    Christophe Dez
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
    EMBO J 25:1534-46. 2006
    ..Localization of pre-ribosomes to this focus was lost in sda1-2 strains lacking Trf4p or Rrp6p. We designate this nucleolar focus the No-body and propose that it represents a site of pre-ribosome surveillance...
  44. ncbi Accurate processing of a eukaryotic precursor ribosomal RNA by ribonuclease MRP in vitro
    Z Lygerou
    European Molecular Biology Laboratory, Heidelberg, Germany
    Science 272:268-70. 1996
    ..Biochemical purification and the use of extracts depleted of the MRP RNA demonstrate that endonucleolytic cleavage of the pre-rRNA is directly mediated by RNase MRP. This establishes a role for RNase MRP in the nucleolus...
  45. pmc Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits
    Marie Françoise O'Donohue
    Laboratoire de Biologie Moléculaire des Eucaryotes, Université de Toulouse UPS and Centre National de La Recherche Scientifique, F 31000 Toulouse, France
    J Cell Biol 190:853-66. 2010
    ..Interestingly, the functional dichotomy of RPS proteins reported in this study is correlated with the mutation frequency of RPS genes in Diamond-Blackfan anemia...
  46. ncbi The multifunctional nucleolus
    Francois Michel Boisvert
    Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    Nat Rev Mol Cell Biol 8:574-85. 2007
    ....
  47. pmc MicroRNAs with a nucleolar location
    Joan C Ritland Politz
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    RNA 15:1705-15. 2009
    ....
  48. ncbi The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks
    Michael Kruhlak
    Experimental Immunology, NCI
    Nature 447:730-4. 2007
    ..Our data reveal a novel ATM/NBS1/MDC1-dependent pathway that shuts down ribosomal gene transcription in response to chromosome breaks...
  49. ncbi A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis
    François Dragon
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520 8040, USA
    Nature 417:967-70. 2002
    ..We have termed this large RNP the small subunit (SSU) processome...
  50. pmc Nucleolus: the fascinating nuclear body
    Valentina Sirri
    Nuclei and Cell Cycle, CNRS, Universite Paris VI, Universite Paris VII, Institut Jacques Monod, 2 place Jussieu, 75251, Paris Cedex 05, France
    Histochem Cell Biol 129:13-31. 2008
    ..Perspectives on the evolution of this research area are proposed...
  51. pmc Assembly and disassembly of the nucleolus during the cell cycle
    Danièle Hernandez-Verdun
    Universite Paris Diderot, France
    Nucleus 2:189-94. 2011
    ..The dynamics of assembly/disassembly of the nucleolus is time and space regulated...
  52. ncbi Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis
    Xuejun Yuan
    Division of Molecular Biology of the Cell II, German Cancer Research Center, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Mol Cell 19:77-87. 2005
    ..The striking correlation between perturbation of nucleolar function, elevated levels of p53, and induction of cell suicide supports the view that the nucleolus is a stress sensor that regulates p53 activity...
  53. pmc Rrs1 is involved in endoplasmic reticulum stress response in Huntington disease
    Alisia Carnemolla
    Sector of Neurobiology, International School for Advanced Studies, Via Beirut 2 4, 34151 Trieste, Italy
    J Biol Chem 284:18167-73. 2009
    ..Interestingly, we demonstrate that ER stress is an early event in a presymptomatic HD mouse model that persists throughout the life span of the rodent. We further show that ER stress also occurs in postmortem brains of HD patients...
  54. pmc MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells
    Joan C Ritland Politz
    Department of Biochemistry and Molecular Pharmacology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 103:18957-62. 2006
    ..These results suggest that miR-206 may associate both with nascent ribosomes in the nucleolus and with exported, functional ribosomes in the cytoplasm...
  55. ncbi Nucleolin: a multiFACeTed protein
    Fabien Mongelard
    Laboratoire Joliot Curie, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69007 Lyon, France
    Trends Cell Biol 17:80-6. 2007
    ..These new properties could help reconcile discrepancies in the literature regarding the role of nucleolin in transcription...
  56. pmc High-resolution whole-genome sequencing reveals that specific chromatin domains from most human chromosomes associate with nucleoli
    Silvana van Koningsbruggen
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Biol Cell 21:3735-48. 2010
    ..Unexpectedly, both the direct DNA sequencing and fluorescence photoactivation data show that certain chromatin loci can specifically associate with either the nucleolus, or the nuclear envelope...
  57. pmc Initial genomics of the human nucleolus
    Attila Nemeth
    Department of Biochemistry III, University of Regensburg, Regensburg, Germany
    PLoS Genet 6:e1000889. 2010
    ..Altogether, our findings describe the nature of DNA sequences associated with the human nucleolus and provide insights into the function of the nucleolus in genome organization and establishment of nuclear architecture...
  58. pmc Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system
    Olivier Gadal
    Laboratoire de Physio Génomique, Service de Biochimie et Génétique Moléculaire, CEA Saclay, Batiment 142, F 91191 Gif sur Yvette, France
    EMBO J 21:5498-507. 2002
    ..Conversely, rpa34-Delta mutants lacking Rpa34, a non-essential subunit associated with Rpa49, are lethal in top1-Delta but not in top3-Delta...
  59. ncbi Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus
    S Llanos
    Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK
    Nat Cell Biol 3:445-52. 2001
    ..We therefore propose that nucleolar localization is not essential for ARF function but may enhance the availability of ARF to inhibit MDM2...
  60. pmc Proteomics analysis of nucleolar SUMO-1 target proteins upon proteasome inhibition
    Vittoria Matafora
    Division of Genomics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy
    Mol Cell Proteomics 8:2243-55. 2009
    ..The analysis of the nature of the SUMO-1 targets identified in this study strongly indicates that sumoylation, acting in coordination with the ubiquitin-proteasome system, regulates the maintenance of nucleolar integrity...
  61. pmc SUMO-1 modification alters ADAR1 editing activity
    Joana M P Desterro
    Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649 028 Lisboa, Portugal
    Mol Biol Cell 16:5115-26. 2005
    ..Moreover, modification of wild-type recombinant ADAR1 by SUMO-1 reduces the editing activity of the enzyme in vitro. Taken together these data suggest a novel role for sumoylation in regulating RNA-editing activity...
  62. pmc Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
    Mohammed Abdullahel Amin
    Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
    Biochem J 415:345-51. 2008
    ..These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells...
  63. ncbi The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions
    A G Balakin
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003, USA
    Cell 86:823-34. 1996
    ..Binding of this or another common small nucleolar ribonucleoprotein particle protein is predicted to be a critical entry point to snoRNA posttranscriptional life, including precise formation of the snoRNA 3' end...
  64. ncbi Tryptophans 286 and 288 in the C-terminal region of protein B23.1 are important for its nucleolar localization
    Yuki Nishimura
    Department of Sustainable Resource Science, Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514 8507, Japan
    Biosci Biotechnol Biochem 66:2239-42. 2002
    ..1 mutant proteins fused with green fluorescent protein in HeLa cells. The results suggested that the two C-terminal tryptophan residues (Trp-286 and Trp-288) of protein B23.1 were important in this phenomenon...
  65. pmc An intranucleolar body associated with rDNA
    Saskia Hutten
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, UK
    Chromosoma 120:481-99. 2011
    ..INBs are abundant in nucleoli of unstressed cells during S phase and localise in close proximity to rDNA with heterochromatic features. The data suggest the INB is linked with regulation of rDNA transcription and/or maintenance of rDNA...
  66. ncbi Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability
    L Latonen
    Molecular Cancer Biology Program and Haartman Institute, University of Helsinki, Helsinki, Finland
    Oncogene 30:790-805. 2011
    ..These findings show that the nucleolus controls protein and RNA surveillance and export by the ubiquitin pathway in a previously unidentified manner, and provide mechanistic insight into the cellular effects of PIs...
  67. ncbi Dynamic proteomics of individual cancer cells in response to a drug
    A A Cohen
    Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Science 322:1511-6. 2008
    ..We identify proteins whose dynamics differ widely between cells, in a way that corresponds to the outcomes-cell death or survival. This opens the way to understanding molecular responses to drugs in individual cells...
  68. pmc Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells
    Hui Gao
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Biol Cell 14:4826-34. 2003
    ..Together these data support a role for Mus81 in recombination repair in higher eukaryotes...
  69. ncbi Ribosome assembly in eukaryotes
    Micheline Fromont-Racine
    Genetique des Interactions Macromoleculaires, Institut Pasteur, 75724 Cedex 15, Paris, France
    Gene 313:17-42. 2003
    ..Proteomic approaches have provided the first indications for a ribosome assembly pathway in eukaryotes and confirmed the dynamic character of the whole process...
  70. ncbi Immunopurification of heterogeneous nuclear ribonucleoprotein particles reveals an assortment of RNA-binding proteins
    S Pinol-Roma
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208
    Genes Dev 2:215-27. 1988
    ..We describe a general method for the large-scale purification of hnRNP proteins by affinity chromatography on ssDNA columns and its use for the production of new monoclonal antibodies to hnRNP proteins...
  71. pmc Characterization of AtNUC-L1 reveals a central role of nucleolin in nucleolus organization and silencing of AtNUC-L2 gene in Arabidopsis
    Frederic Pontvianne
    Unité Mixte de Recherche Centre National de la Recherche Scientifique 5096, Universite de Perpignan, Perpignan, 66860 Perpignan, France
    Mol Biol Cell 18:369-79. 2007
    ..This work is the first description of a higher eukaryotic organism with a disrupted nucleolin-like gene and defines a new role for nucleolin in nucleolus structure and rDNA chromatin organization...
  72. pmc Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP
    Nicholas J Watkins
    Max Planck Institut fur biophysikalische Chemie, Abteilung Zelluläre Biochemie, D 37070, Gottingen, Germany
    Mol Cell Biol 22:8342-52. 2002
    ..5K, to the snoRNA. Indeed, the sequence of stem II was essential for nucleolar localization of U14 snoRNA microinjected into HeLa cells. Thus, the conserved sequence of stem II determines the specific assembly of the box C/D snoRNP...
  73. pmc The role of Cdc55 in the spindle checkpoint is through regulation of mitotic exit in Saccharomyces cerevisiae
    Christopher M Yellman
    Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, University of Virginia, Charlottesville, VA 22908, USA
    Mol Biol Cell 17:658-66. 2006
    ..Interestingly, the B-type cyclin Clb2 is partially stable during premature activation of mitotic exit in a cdc55 mutant, indicating mitotic exit is incomplete...
  74. pmc Bop1 is a mouse WD40 repeat nucleolar protein involved in 28S and 5. 8S RRNA processing and 60S ribosome biogenesis
    Z Strezoska
    Department of Molecular Genetics, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60607 7170, USA
    Mol Cell Biol 20:5516-28. 2000
    ..These studies thus identify Bop1 as a novel, nonribosomal mammalian protein that plays a key role in the formation of the mature 28S and 5.8S rRNAs and in the biogenesis of the 60S ribosomal subunit...
  75. pmc Nucleolar assembly of the rRNA processing machinery in living cells
    T M Savino
    Institut Jacques Monod, UMR 7592, 2 place Jussieu, 75251 Paris Cedex 05, France
    J Cell Biol 153:1097-110. 2001
    ..This demonstrates the formation of one functional domain by cooperative interactions between different chromosome territories...
  76. ncbi Nucleolar proteomics and viral infection
    Julian A Hiscox
    Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK
    Proteomics 10:4077-86. 2010
    ....
  77. ncbi In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolus
    Tao Wei
    Department of Cell Biology, School of Life Sciences, Peking University, 100871, China
    J Cell Sci 116:1117-25. 2003
    ..The DNA clumps within the fibrillar center were probably the anchoring sites for rDNA arrangement...
  78. ncbi Immobilization of proteins in the nucleolus by ribosomal intergenic spacer noncoding RNA
    Timothy E Audas
    Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada
    Mol Cell 45:147-57. 2012
    ..We propose a model whereby protein immobilization by ncRNA is a posttranslational regulatory mechanism...
  79. pmc NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression
    Erwana Harscoët
    INRA, IJPB, UMR 1318 INRA AgroParisTech, Versailles, France
    PLoS ONE 5:e12829. 2010
    ....
  80. pmc Proinsulin C-peptide regulates ribosomal RNA expression
    Emma Lindahl
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE 171 77 Stockholm, Sweden
    J Biol Chem 285:3462-9. 2010
    ..This regulation of ribosomal RNA provides a mechanism by which C-peptide can exert transcriptional effects and implies that the peptide has growth factor activity...
  81. ncbi Proteomic profiling of the human T-cell nucleolus
    Mohamed Ali Jarboui
    Centre for Research in Infectious Diseases, School of Medicine and Medical Science, University College Dublin, Belfield, Dublin 4, Ireland
    Mol Immunol 49:441-52. 2011
    ....
  82. ncbi Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiae
    Daisuke Morita
    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama 1 4 4, Higashi Hiroshima 739 8528, Japan
    J Biol Chem 277:28780-6. 2002
    ..An analysis of processing intermediates by primer extension shows that the Rpf2p depletion leads to an accumulation of 27 SB pre-rRNA, suggesting that Rpf2p is required for the processing of 27 SB into 25 S rRNA...
  83. ncbi Tagging of functional ribosomes in living cells by HaloTag® technology
    Simone Gallo
    San Raffaele Scientific Institute DIBIT, Via Olgettina 58, 20132, Milan, Italy
    In Vitro Cell Dev Biol Anim 47:132-8. 2011
    ..In addition, the level of overexpression and degradation may vary upon different constructs. In summary, HaloTag® technology is highly suitable to ribosome tagging, but requires prior characterization for each construct...
  84. ncbi Nuclear DNA helicase II (RNA helicase A) binds to an F-actin containing shell that surrounds the nucleolus
    Suisheng Zhang
    Department of Biochemistry, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D 07708 Jena, Germany
    Exp Cell Res 293:248-58. 2004
    ..Taken together, these results support the idea that an actin-based filamentous network may anchor NDH II at the nucleolar periphery for pre-ribosomal RNA processing, ribosome assembly, and/or transport...
  85. pmc NoD: a Nucleolar localization sequence detector for eukaryotic and viral proteins
    Michelle S Scott
    Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
    BMC Bioinformatics 12:317. 2011
    ..Although many proteins have been reported to contain NoLSs, the systematic characterization of this group of targeting motifs has only recently been carried out...
  86. ncbi Novel variants of muscle calpain 3 identified in human melanoma cells: cisplatin-induced changes in vitro and differential expression in melanocytic lesions
    D Moretti
    Department of Physiopathology, Experimental Medicine and Public Health, University of Siena, Italy
    Carcinogenesis 30:960-7. 2009
    ..On the whole, our observations suggest that calpain 3 variants can play a proapoptotic role in melanoma cells and its downregulation, as observed in highly aggressive lesions, could contribute to melanoma progression...
  87. pmc Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteins
    Tim Krüger
    Department of Cell and Developmental Biology, Biocenter, University of Wurzburg, Wurzburg, Germany
    J Cell Biol 177:573-8. 2007
    ....
  88. pmc Discrete foci containing RNase A are found in nucleoli of HeLa cells after aging in culture
    M Costanzo
    Laboratorio di Biologia Cellulare e Neurobiologia, Dipartimento di Biologia Animale, Universita di Pavia, Italy
    Eur J Histochem 55:e15. 2011
    ..These bodies also contain RNA and lack ribosomal S3 proteins, and may represent either simple storage sites or areas where RNA degradation takes place...
  89. ncbi Involvement of the plant nucleolus in virus and viroid infections: parallels with animal pathosystems
    M E Taliansky
    Scottish Crop Research Institute, Invergowrie, Dundee, United Kingdom
    Adv Virus Res 77:119-58. 2010
    ..Investigating the interactions between plant viruses and the nucleolus will facilitate the design of novel strategies to control plant virus infections...
  90. pmc Silencing of ribosomal protein S9 elicits a multitude of cellular responses inhibiting the growth of cancer cells subsequent to p53 activation
    Mikael S Lindström
    Department of Oncology Pathology, Karolinska Institutet, Stockholm, Sweden
    PLoS ONE 5:e9578. 2010
    ..The effects of ribosomal protein loss in cultured mammalian cells have not been thoroughly investigated. Here we characterize the cellular stress response caused by depletion of ribosomal protein S9 (RPS9)...
  91. ncbi Systematic identification of pathways that couple cell growth and division in yeast
    Paul Jorgensen
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
    Science 297:395-400. 2002
    ..Cell growth and division appear to be coupled by multiple conserved mechanisms...
  92. ncbi Nucleolar disruption in dopaminergic neurons leads to oxidative damage and parkinsonism through repression of mammalian target of rapamycin signaling
    Claus Rieker
    Division of Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg, Germany
    J Neurosci 31:453-60. 2011
    ..Collectively, these observations suggest that the interplay between nucleolar dysfunction and increased oxidative stress, involving p53 and mTOR signaling, may constitute a destructive axis in experimental and sporadic PD...
  93. ncbi Identification of a cryptic nucleolar-localization signal in MDM2
    M A Lohrum
    NCI FCRDC, Building 560, Room 22 96, West 7th Street, Frederick, Maryland 21702 1201, USA
    Nat Cell Biol 2:179-81. 2000
  94. pmc Perturbation of 60 S ribosomal biogenesis results in ribosomal protein L5- and L11-dependent p53 activation
    Xiao Xin Sun
    Department of Molecular and Medical Genetics, School of Medicine, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Biol Chem 285:25812-21. 2010
    ..These results demonstrate that NEDDylation of L11 plays a critical role in mediating p53 activation in response to perturbation of ribosomal biogenesis...
  95. ncbi Expression and localization of Werner syndrome protein is modulated by SIRT1 and PML
    Rasa Vaitiekunaite
    Department of Tumor Biology, Maria Skłodowska Curie Memorial Cancer Center, ul Wybrzeze Armii Krajowej 15, 44 101 Gliwice, Poland
    Mech Ageing Dev 128:650-61. 2007
    ..Our data indicate for the first time that SIRT1 protein may be functionally associated with WRN and BLM helicases and that some major SIRT1 functions may not require its deacetylase activity...
  96. ncbi Autoantibody profiles in systemic sclerosis: predictive value for clinical evaluation and prognosis
    Yasuhito Hamaguchi
    Department of Dermatology, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa 920 8641, Japan
    J Dermatol 37:42-53. 2010
    ....
  97. pmc Alterations in nucleolar structure and gene expression programs in prostatic neoplasia are driven by the MYC oncogene
    Cheryl M Koh
    Department of Pathology, The Johns Hopkins University, School of Medicine, Baltimore, MD 21231, USA
    Am J Pathol 178:1824-34. 2011
    ....
  98. ncbi Both carboxy-terminus NES motif and mutated tryptophan(s) are crucial for aberrant nuclear export of nucleophosmin leukemic mutants in NPMc+ AML
    Brunangelo Falini
    Institute of Hematology, University of Perugia, 06122 Perugia, Italy
    Blood 107:4514-23. 2006
    ..These findings indicate that potential therapeutic strategies aimed to retarget NPM to its physiological sites will have to overcome 2 obstacles, the new NES motif and the mutated tryptophan(s) at the NPM mutant C-terminus...
  99. pmc Regulation of nucleolar signalling to p53 through NEDDylation of L11
    Anders Sundqvist
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, Scotland, UK
    EMBO Rep 10:1132-9. 2009
    ..By controlling the correct localization and stability of L11, NEDD8 acts as a crucial, new regulator of nucleolar signalling to p53...
  100. ncbi A nucleolar targeting signal in PML-I addresses PML to nucleolar caps in stressed or senescent cells
    Wilfried Condemine
    CNRS Université de Paris 7 UMR7151, Equipe Labellisee par la Ligue Contre le Cancer, Hopital St Louis, 1 Av C Vellefaux 75475, Paris Cedex 10, France
    J Cell Sci 120:3219-27. 2007
    ..Later, poly-ubiquitin conjugates are found on the outer shell or within most of these senescence-associated PML bodies. Thus, unexpectedly, the scarcely studied PML-I isoform links PML bodies, nucleolus, senescence and proteolysis...
  101. pmc Dynamic behavior of Arabidopsis eIF4A-III, putative core protein of exon junction complex: fast relocation to nucleolus and splicing speckles under hypoxia
    O A Koroleva
    Department of Cell Biology, John Ines Centre, Colney, Norwich NR4 7UH, United Kingdom
    Plant Cell 21:1592-606. 2009
    ..Sequestration of eIF4A-III into nuclear pools with different mobility is likely to reflect the transcriptional and mRNA processing state of the cell...

Research Grants39

  1. NORMAL AND MUTANT CHORION GENES OF DROSOPHILA
    Allan Spradling; Fiscal Year: 2003
    ..Several candidate genes that are known to be necessary for nurse cell nucleolus formation, or for normal nurse cell chromosome structure, will be tested...
  2. CANCER RESEARCH PROGRAM
    Harris Busch; Fiscal Year: 1991
    ....
  3. A Novel HTP Assay for Identification of Compounds that Reduce PNC Prevalence
    Sui Huang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  4. Survival signaling in CNS neurons exposed to DNA damage
    Michal Hetman; Fiscal Year: 2008
    ..The proposed research may result in identification of new drug targets for treatment of neurological diseases and/or deleterious neurological side effects of cancer therapy. ..
  5. Spondyloarthritis 2006; The Unmet Needs
    John Reveille; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  6. RNA Transport Within the Nucleus
    Thoru Pederson; Fiscal Year: 2005
    ..Another aspect of the project will address the intranuclear traffic of these RNAs in relation to proteins known to be involved in nuclear import or RNA export, i.e. the Ran-GTPase and TAP. ..
  7. The Perinucleolar Compartment as Breast Cancer Marker
    Sui Huang; Fiscal Year: 2005
    ..We expect the proposed studies will clarify whether the PNC, an easily detectable cellular structure, could improve the accuracy of breast cancer prognosis and prediction when used in combination with existing markers. ..
  8. Biogenesis of Eukaryotic Ribosomes
    SUSAN GERBI; Fiscal Year: 2004
    ..The proposed studies should elucidate the importance of dynamic RNA-RNA interactions during ribosome biogenesis, a fundamental process essential for cell growth and viability. ..
  9. PERINUCLEOLAR COMPARTMENT AND CANCER
    Sui Huang; Fiscal Year: 2003
    ..The results of these experiments will provide an important foundation to address the significance of the PNC in malignant cells. ..
  10. AUTOANTIGEN RNA HELICASE II/GU
    Benigno Valdez; Fiscal Year: 2003
    ..This study may also provide an impetus for understanding the pathological roles of RH-II/Gu on the elicitation of autoimmune responses. ..
  11. RNA PROCESSING AND RIBONUCLEOPROTEIN
    Thoru Pederson; Fiscal Year: 2001
    ..The same studies should provide information on sequences required for nuclear export. These two inter-related projects test the hypothesis that the nucleolus is a more pluri-functional organelle than previously thought. ..
  12. Gene Amplification-Sciarid DNA Puffs
    SUSAN GERBI; Fiscal Year: 2006
    ..Information gained from the unique Sciara system should behelpful in understanding the mechanisms used by metazoa to specify an origin and to regulate its activation. ..
  13. FAMILY STUDIES OF THE GENETICS OF ANKYLOSING SPONDYLITIS
    John Reveille; Fiscal Year: 2003
    ..abstract_text> ..
  14. Stem-like Cancer Cells in Breast Tumorigenesis
    ROBERT TSAI; Fiscal Year: 2009
    ..abstract_text> ..
  15. Prion-Based Mechanisms in Metazoan Development
    KORIE HANDWERGER; Fiscal Year: 2006
    ..Finally, CePrDps will be examined for their ability to regulate development in the absence of their putative prion domains. ..
  16. Insulin and Oocyte Chromatin Segregation
    Gary Smith; Fiscal Year: 2009
    ..abstract_text> ..
  17. Molecular basis of neuronal dysfunction in AIDS
    Francesca Peruzzi; Fiscal Year: 2008
    ..The outcome of these studies will provide a new information on the pathways which are affected by HIV-1 Tat, and will assist to improve our effort for developing strategies against HIV-1 induced neuronal cell injury. ..
  18. Biochemical Genomics Linking Genes and Activities
    Eric Phizicky; Fiscal Year: 2004
    ..Application of these techniques to other organisms, including humans and pathogens, will greatly accelerate biochemical analysis and can be used to rapidly identify drug targets. ..
  19. Cloning and analysis of centrosome-associated RNA
    MARK ALLIEGRO; Fiscal Year: 2007
    ..If our hypothesis is correct, this study will reopen a line of inquiry with impact in areas of cell and developmental biology ranging from control of cell division to evolution of eukaryotes. [unreadable] [unreadable]..
  20. INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLS
    Michael Berns; Fiscal Year: 2001
    ..The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research. ..
  21. Retrotransposons as site-specific gene delivery vectors
    JOHN GOODIER; Fiscal Year: 2003
    ..In addition, I will assay in cell culture the efficiency of the L1 at integrating increasing lengths of exogenous DNA. ..
  22. Telomerase Transport and Targeting
    MICHAEL TERNS; Fiscal Year: 2008
    ..abstract_text> ..
  23. Role of RecQ Helicases to Prevent Senescence By c-Myc
    Carla Grandori; Fiscal Year: 2010
    ..If so, drug inhibitors of WRN function could be proposed for the treatment of cancer. ..
  24. Preventive of Bile Acid-Induced Colonic Oxidative DNA Damage
    Claire Margaret Payne; Fiscal Year: 2010
    ..129 mice) developed in our laboratory;2) NOS-/- k.o./DOC mouse model;3) arginine-enhanced colon tumor ApcMin/+ mouse model developed in our laboratory;4) the AOM mouse model of colon carcinogenesis using A/J mice. ..
  25. Cytochrome C Oxidase I:A Biomarker for Colon Cancer Risk
    Claire Payne; Fiscal Year: 2006
    ..Various molecular and cellular techniques will be used in these studies. ..
  26. LASER PHOTORADIATION THERAPY OF MALIGNANT TUMORS
    Michael Berns; Fiscal Year: 2002
    ..Response will be evaluated at 3 month intervals for up to one year post treatment. ..
  27. BIOREGULATION OF THE SPERMATOGONIAL STEM CELL LINEAGE
    LAURA TRES; Fiscal Year: 2003
    ..2. In the long range, the discovery of ways to prevent and modulate spermatogonial cell death may have significant therapeutic and anticonceptive potentials in humans. ..
  28. INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLS
    Michael Berns; Fiscal Year: 2003
    ..The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research. ..
  29. Recombination and meiotic progression in the mouse
    Laura Reinholdt; Fiscal Year: 2004
    ..Finally, a new, potentially meiotic mutation, Mei5, which may cause a meiotic defect that escapes detection by meiotic checkpoint mechanisms, will be phenotypically characterized and genetically mapped. ..
  30. MHC-Defined and Identical Twin Monkeys for Research
    Joseph Kemnitz; Fiscal Year: 2005
    ..Additionally, genetically identical macaques will have general utility in biomedical research, especially to the neuroscience community. ..
  31. Human Posterior Cricoarytenoid Muscle Aging Mechanisms
    Leslie Malmgren; Fiscal Year: 2005
    ..Since processes such as apoptosis are potential targets for clinical intervention, the proposed studies will be essential to the design of improved strategies for the prevention or treatment of age-related laryngeal dysfunction. ..
  32. PROTEIN PHOSPHATASES AND OOCYTE MEIOSIS
    Gary Smith; Fiscal Year: 2002
    ..These studies will provide novel information concerning control of oocyte maturation and spindle formation that may subsequently influence the developmental competency of resulting embryos. ..
  33. DIRECT TARGET GENES OF THE MYC ONCOPROTEIN
    Carla Grandori; Fiscal Year: 2002
    ..These studies will provide several pieces of the puzzle necessary to understand how Myc functions as an oncogene. ..
  34. Import of RNA into Mitochondria
    MARK SCHMITT; Fiscal Year: 2006
    ..4) Identification of other RNA molecules imported into mitochondria. These experiments should provide a wealth of knowledge about this fundamental process. ..
  35. MITOSIS IN NORMAL AND NEOPLASTIC CELLS
    BILL BRINKLEY; Fiscal Year: 2006
    ..abstract_text> ..
  36. DEVELOPMENTAL REGULATION OF GROWTH, SIZE AND SHAPE
    Nicholas E Baker; Fiscal Year: 2010
    ....
  37. Novel Genes Expressed in Proliferating Endothelial Cells
    MARK ALLIEGRO; Fiscal Year: 2006
    ..abstract_text> ..
  38. YEAST CHROMOSOME STRUCTURE, REPLICATION AND SEGREGATION
    Angelika Amon; Fiscal Year: 2004
    ..Our objective for this meeting is to continue the extremely high level of excitement and scientific excellence that has characterized the past seven conferences. ..
  39. Synapse-to-nucleus signaling components in PSDs
    BRYEN JORDAN; Fiscal Year: 2007
    ..This work will increase our understanding of synaptic function which is critical in learning and memory. ..