intracellular space

Summary

Summary: The area within CELLS.

Top Publications

  1. pmc Growth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations
    Viktor M Boer
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 21:198-211. 2010
  2. pmc Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors
    Mingxing Ouyang
    Department of Bioengineering, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, IL 61801, USA
    Proc Natl Acad Sci U S A 105:14353-8. 2008
  3. pmc Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes
    Marzia Massignani
    Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 5:e10459. 2010
  4. pmc Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions
    Chin Sheng Yu
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30050, Taiwan
    Protein Sci 13:1402-6. 2004
  5. pmc Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells
    Andreas Götz
    Lehrstuhl fur Mikrobiologie, Biozentrum der Universität Würzburg, Wurzburg, Germany
    PLoS ONE 5:e10586. 2010
  6. pmc Multifunctional nanoparticles delivering small interfering RNA and doxorubicin overcome drug resistance in cancer
    Yunching Chen
    Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 285:22639-50. 2010
  7. ncbi CCR5 internalisation and signalling have different dependence on membrane lipid raft integrity
    Clara Moyano Cardaba
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK
    Cell Signal 20:1687-94. 2008
  8. ncbi RNA catalysis through compartmentalization
    Christopher A Strulson
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nat Chem 4:941-6. 2012
  9. ncbi Enhancement of efficiencies of the cellular uptake and gene silencing of chitosan/siRNA complexes via the inclusion of a negatively charged poly(γ-glutamic acid)
    Zi Xian Liao
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Biomaterials 31:8780-8. 2010
  10. ncbi 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces apoptosis by disruption of intracellular calcium homeostasis in human neuronal cell line SHSY5Y
    Antonio Morales-Hernández
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Apoptosis 17:1170-81. 2012

Research Grants

  1. Impact of neuronal chloride transport on treatment of seizures.
    Kevin J Staley; Fiscal Year: 2013
  2. Mapping pH at the surface of individual cell
    Yana K Reshetnyak; Fiscal Year: 2013
  3. Estimating Intracellular Lithium in Brain in vivo by 7Li MRS
    Richard A Komoroski; Fiscal Year: 2010
  4. BLOOD BRAIN BARRIER LARGE NEUTRAL AMINO ACID TRANSPORTER
    William Pardridge; Fiscal Year: 2003
  5. NUMERICAL SIMULATION OF CARDIAC ELECTROPHYSIOLOGY
    Bradley Roth; Fiscal Year: 2005
  6. BIOMEDICAL RESEARCH SUPPORT
    Michael Riley; Fiscal Year: 1992
  7. VOLUME ABSORPTION IN THE RENAL PROXIMAL TUBULE
    James Williams; Fiscal Year: 1992
  8. ENVIRONMENT OF LITHIUM IN RAT BRAIN IN VIVO
    Richard Komoroski; Fiscal Year: 2000
  9. In Vivo Imaging of Retinal Apoptosis Using Activatable Permeation Peptides
    EDWARD BARNETT; Fiscal Year: 2007
  10. GH AND IGF-I TREATMENT OF ELDERLY WOMEN
    Andrew Hoffman; Fiscal Year: 2000

Detail Information

Publications296 found, 100 shown here

  1. pmc Growth-limiting intracellular metabolites in yeast growing under diverse nutrient limitations
    Viktor M Boer
    Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 21:198-211. 2010
    ..The complete data can be accessed at the interactive website http://growthrate.princeton.edu/metabolome...
  2. pmc Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors
    Mingxing Ouyang
    Department of Bioengineering, Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, IL 61801, USA
    Proc Natl Acad Sci U S A 105:14353-8. 2008
    ..In summary, ECFP/YPet may serve as a general FRET pair for the development of highly sensitive biosensors to allow the determination of molecular hierarchies at subcellular locations in live cells...
  3. pmc Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes
    Marzia Massignani
    Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom
    PLoS ONE 5:e10459. 2010
    ..We demonstrate herein that this hurdle can be overcome by effective cytosolic delivery...
  4. pmc Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions
    Chin Sheng Yu
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30050, Taiwan
    Protein Sci 13:1402-6. 2004
    ..Because of its simplicity, this approach can be easily extended to other organisms and should be a useful tool for the high-throughput and large-scale analysis of proteomic and genomic data...
  5. pmc Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells
    Andreas Götz
    Lehrstuhl fur Mikrobiologie, Biozentrum der Universität Würzburg, Wurzburg, Germany
    PLoS ONE 5:e10586. 2010
    ..Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens...
  6. pmc Multifunctional nanoparticles delivering small interfering RNA and doxorubicin overcome drug resistance in cancer
    Yunching Chen
    Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 285:22639-50. 2010
    ..The activity and the toxicity of LPD- and LPD-II-mediated therapy are compared...
  7. ncbi CCR5 internalisation and signalling have different dependence on membrane lipid raft integrity
    Clara Moyano Cardaba
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK
    Cell Signal 20:1687-94. 2008
    ....
  8. ncbi RNA catalysis through compartmentalization
    Christopher A Strulson
    Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nat Chem 4:941-6. 2012
    ..This rate enhancement can be tuned by the relative volumes of the two phases in the ATPS. Our observations support the importance of compartmentalization in the attainment of function in an RNA World as well as in modern biology...
  9. ncbi Enhancement of efficiencies of the cellular uptake and gene silencing of chitosan/siRNA complexes via the inclusion of a negatively charged poly(γ-glutamic acid)
    Zi Xian Liao
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Biomaterials 31:8780-8. 2010
    ..The aforementioned results suggest that CS/siRNA/γ-PGA complexes can be an efficient vector for siRNA transfection...
  10. ncbi 2,3,7,8-Tetrachlorodibenzo-p-dioxin induces apoptosis by disruption of intracellular calcium homeostasis in human neuronal cell line SHSY5Y
    Antonio Morales-Hernández
    Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain
    Apoptosis 17:1170-81. 2012
    ..Altogether these results support the hypothesis that TCDD toxicity in SHSY5Y neuroblastoma cells provokes the disruption of calcium homeostasis, probably affecting membrane structural integrity, leading to an apoptotic process...
  11. pmc Characterization of the mechanisms of the increase in PPARδ expression induced by digoxin in the heart using the H9c2 cell line
    Zhih Cherng Chen
    Department of Cardiology, Chi Mei Medical Center, Yong Kang City, Tainan County, Taiwan
    Br J Pharmacol 163:390-8. 2011
    ..The aim of the present study was to investigate possible mechanisms for this effect of digoxin using the H9c2 cell line cultured in high glucose (HG) conditions...
  12. pmc Investigating the robustness of the classical enzyme kinetic equations in small intracellular compartments
    Ramon Grima
    Centre for Systems Biology at Edinburgh, University of Edinburgh, UK
    BMC Syst Biol 3:101. 2009
    ....
  13. pmc Novel genetic tools for diaminopimelic acid selection in virulence studies of Yersinia pestis
    David M Bland
    Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, United States of America
    PLoS ONE 6:e17352. 2011
    ..Thus, diaminopimelic acid selection for single or multi-copy genetic systems in Yersinia pestis offers an improved alternative to antibiotics for in vivo studies that causes minimal disruption to virulence...
  14. pmc Na/K pump current and [Na](i) in rabbit ventricular myocytes: local [Na](i) depletion and Na buffering
    Sanda Despa
    Department of Physiology, Loyola University Chicago, Maywood, Illinois 60153, USA
    Biophys J 84:4157-66. 2003
    ..I(pump) integrals and [Na](i) decline were used to estimate intracellular Na buffering, which is slight (1.39 +/- 0.09)...
  15. pmc Loss-of-function mutations in Rab escort protein 1 (REP-1) affect intracellular transport in fibroblasts and monocytes of choroideremia patients
    Natalia V Strunnikova
    Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 4:e8402. 2009
    ..Under-prenylation of certain Rabs, as a result of loss of function mutations in REP-1, could affect vesicular trafficking, exocytosis and secretion in peripheral cells of CHM patients...
  16. pmc Identification of small RNAs in Francisella tularensis
    Guillaume Postic
    INSERM U1002, Paris, France
    BMC Genomics 11:625. 2010
    ..Relatively little is known about the regulatory networks existing in this organism that allows it to survive in a wide array of environments and no sRNA regulators have been identified so far...
  17. pmc Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
    Jörg Schaber
    Theoretical Biophysics, Humboldt University, Invaliden Str 42, 10115 Berlin, Germany
    Eur Biophys J 39:1547-56. 2010
    ..This finding suggests that mechanical properties of the membrane trigger HOG pathway activation, which can be represented and quantitatively modeled by turgor...
  18. ncbi Homeostasis established by coordination of subcellular compartment plasticity improves spike encoding
    Na Chen
    State Key Labs for Macrobiomolecules and Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, The People s Republic of China
    J Cell Sci 121:2961-71. 2008
    ..Our study of neuronal homeostasis that is fulfilled by rapidly coordinating subcellular compartments to improve neuronal encoding sheds light on exploring homeostatic mechanisms in other cell types...
  19. pmc Non-cardiomyocytes influence the electrophysiological maturation of human embryonic stem cell-derived cardiomyocytes during differentiation
    Changsung Kim
    Center for Neuroscience, Aging and Stem Cell Research, Burnham Institute for Medical Research, La Jolla, California 92037, USA
    Stem Cells Dev 19:783-95. 2010
    ..Defining the nature of these extrinsic signals will aid in the directed maturation of immature hESC-CMs to mitigate arrhythmogenic risks of cell-based therapies...
  20. pmc Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient
    Krishnan Venkataraman
    Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030 3501, USA
    Biochem J 397:461-71. 2006
    ..These results suggest that export of Sphk-1a occurs under physiological conditions and may contribute to the establishment of the vascular S1P gradient...
  21. pmc Structural and functional deficits in a neuronal calcium sensor-1 mutant identified in a case of autistic spectrum disorder
    Mark T W Handley
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool, United Kingdom
    PLoS ONE 5:e10534. 2010
    ..From these data we speculate that impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain...
  22. ncbi Rapid decrease of intracellular pH associated with inhibition of Na+/H+ exchanger precedes apoptotic events in the MNK45 and MNK74 gastric cancer cell lines treated with 2-aminophenoxazine-3-one
    Hiroyuki Nagata
    Fourth Department of Surgery, Tokyo Medical University, Ibaragi Medical Center, 3 20 1 Chuo, Ami, Inashiki, Ibaraki 300 0395, Japan
    Oncol Rep 25:341-6. 2011
    ..Phx-3 is a good candidate for therapeutics of gastric cancer that is intractable to conventional chemopreventive therapies...
  23. pmc Connecting extracellular metabolomic measurements to intracellular flux states in yeast
    Monica L Mo
    Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA
    BMC Syst Biol 3:37. 2009
    ..Extracellular metabolome or metabolic profiling data, in particular, can provide an insightful view of intracellular physiological states in a noninvasive manner...
  24. pmc Salt stress-induced cell death in the unicellular green alga Micrasterias denticulata
    Matthias Josef Affenzeller
    Plant Physiology Division, Cell Biology Department, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria
    J Exp Bot 60:939-54. 2009
    ..Respiration, photosynthetic activity, and pigment composition indicated an active metabolism which supports programmed rather than necrotic cell death in Micrasterias after salt stress...
  25. pmc Tyrosine phosphorylation of Sprouty proteins regulates their ability to inhibit growth factor signaling: a dual feedback loop
    Jacqueline M Mason
    Division of Hematology Oncology, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Mol Biol Cell 15:2176-88. 2004
    ..Thus, these data highlight a novel negative and positive regulatory loop that allows for the controlled, homeostatic inhibition of RTK signaling...
  26. ncbi Dynactin
    Trina A Schroer
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    Annu Rev Cell Dev Biol 20:759-79. 2004
    ....
  27. ncbi On the origin of the extracellular action potential waveform: A modeling study
    Carl Gold
    Computation and Neural Systems, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA
    J Neurophysiol 95:3113-28. 2006
    ..Interestingly, each cell's dendritic morphology had very little impact on the EAP waveform. The model also demonstrates that the varied composition of ionic currents in different cells is reflected in the features of the EAP...
  28. ncbi Mechanisms of cellular uptake and intracellular trafficking with chitosan/DNA/poly(γ-glutamic acid) complexes as a gene delivery vector
    Shu fen Peng
    Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
    Biomaterials 32:239-48. 2011
    ..Knowledge of these mechanisms involving CS-based complexes containing γ-PGA is critical for the development of an efficient vector for DNA transfection...
  29. ncbi The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol
    K Teskac
    University of Ljubljana, Faculty of Pharmacy, Askerceva 7, Ljubljana, Slovenia
    Int J Pharm 390:61-9. 2010
    ..The cytostatic effect of SLN-RSV was much more expressed than that of RSV in solution. Delivery of RSV by SLN contributes to effectiveness of RSV on decreasing cell proliferation, with potential benefits for prevention of skin cancer...
  30. ncbi A chloroplast-localized and auxin-induced glutathione S-transferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco
    Suja George
    Department of Plant Molecular Biology, M S Swaminathan Research Foundation, Taramani Institutional Area, Chennai 600 113, India
    J Plant Physiol 167:311-8. 2010
    ..The peroxidase activity of PjGSTU1 and its localization in the chloroplast indicates a possible role for PjGSTU1 in ROS removal...
  31. ncbi Intracellular galectin-9 activates inflammatory cytokines in monocytes
    Ai Matsuura
    First Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan
    Genes Cells 14:511-21. 2009
    ..Our results indicate that galectin-9 is a LPS-responsive factor, and further demonstrate that intracellular galectin-9 transactivates inflammatory cytokine genes in monocytes through direct physical interaction with NF-IL6...
  32. ncbi CFTR is involved in the fine tuning of intracellular redox status: physiological implications in cystic fibrosis
    Christophe Duranton
    Faculties of Sciences LP2M CNRS 3472, University of Nice Sophia Antipolis, Nice, France
    Am J Pathol 181:1367-77. 2012
    ..We conclude that CFTR controls HIF stabilization through control of the level of intracellular ROS that act as signaling agents in the HIF-1 pathway...
  33. ncbi Formation of protein complexes in crowded environments--from in vitro to in vivo
    Yael Phillip
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    FEBS Lett 587:1046-52. 2013
    ..Obtaining quantitative biochemical information on reactions inside living cells is currently a main challenge of the field, as the complexity of the intracellular milieu was what motivated crowding research to begin with...
  34. pmc Fibroblast circadian rhythms of PER2 expression depend on membrane potential and intracellular calcium
    Takako Noguchi
    Department of Psychiatry and Center for Chronobiology, University of California, San Diego, La Jolla, USA
    Chronobiol Int 29:653-64. 2012
    ..These results suggest that the circadian clock in fibroblasts, as in SCN neurons, is regulated by membrane potential and Ca(2+). Changes in intracellular Ca(2+) may mediate the effects of membrane potential observed in this study...
  35. pmc siRNA-like double-stranded RNAs are specifically protected against degradation in human cell extract
    John A H Hoerter
    Department of Chemistry, University of Michigan, Ann Arbor, Michigan, United States of America
    PLoS ONE 6:e20359. 2011
    ..These results suggest that RNAi effector RNAs are specifically protected in the cellular environment and may provide an explanation for recent results showing that unmodified siRNAs in cells persist intact for extended periods of time...
  36. pmc Substrate stiffness increases twitch power of neonatal cardiomyocytes in correlation with changes in myofibril structure and intracellular calcium
    Anthony G Rodriguez
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Biophys J 101:2455-64. 2011
    ..Altogether, these findings indicate that cardiomyocytes respond to substrate stiffness with biomechanical and biochemical changes that lead to an increase in cardiac contractility...
  37. pmc ATP binding cassette transporter G1 (ABCG1) is an intracellular sterol transporter
    Elizabeth J Tarling
    Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 108:19719-24. 2011
    ..Our data demonstrate that ABCG1 is an intracellular sterol transporter that localizes to endocytic vesicles to facilitate the redistribution of specific intracellular sterols away from the endoplasmic reticulum (ER)...
  38. pmc Alpha-arrestins Aly1 and Aly2 regulate intracellular trafficking in response to nutrient signaling
    Allyson F O'Donnell
    Department of Biology, Stanford University, Stanford, CA 94305 5020, USA
    Mol Biol Cell 21:3552-66. 2010
    ..This work defines a new role for arrestins in membrane trafficking and offers insight into how α-arrestins coordinate signaling events with protein trafficking...
  39. pmc Mast cells elicit proinflammatory but not type I interferon responses upon activation of TLRs by bacteria
    Nicole Dietrich
    Department of Molecular Immunology, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany
    Proc Natl Acad Sci U S A 107:8748-53. 2010
    ....
  40. pmc Farnesol-induced apoptosis in Candida albicans is mediated by Cdr1-p extrusion and depletion of intracellular glutathione
    Jingsong Zhu
    Department of Oncology and Diagnostic Sciences, University of Maryland, Baltimore, Maryland, United States of America
    PLoS ONE 6:e28830. 2011
    ..albicans. Understanding of the mechanisms underlying farnesol-cytotoxicity in C. albicans may lead to the development of this redox-cycling agent as an alternative antifungal agent...
  41. pmc Linking the transcriptional profiles and the physiological states of Mycobacterium tuberculosis during an extended intracellular infection
    Kyle H Rohde
    Department of Microbiology and Immunology, Cornell University, Ithaca, New York, United States of America
    PLoS Pathog 8:e1002769. 2012
    ..The integration of molecular and cellular biology together with transcriptional profiling and systems analysis offers unique insights into the host-driven responses of intracellular pathogens such as M. tuberculosis...
  42. pmc Inhibition of ABCB1 (MDR1) expression by an siRNA nanoparticulate delivery system to overcome drug resistance in osteosarcoma
    Michiro Susa
    Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS ONE 5:e10764. 2010
    ..However, for patients who progress after chemotherapy, its effectiveness diminishes due to the emergence of multi-drug resistance (MDR) after prolonged therapy...
  43. ncbi CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion
    Fabrizio Montecucco
    Division of Cardiology, Foundation for Medical Researches, Department of Internal Medicine, University Hospital, Geneva, Switzerland
    J Mol Cell Cardiol 46:612-20. 2009
    ..Our data suggest that JWH-133 administration during ischemia reduces the infarct size in a mouse model of myocardial ischemia/reperfusion through a direct cardioprotective activity on cardiomyocytes and neutrophils...
  44. ncbi Protective role of fructose in the metabolism of astroglial C6 cells exposed to hydrogen peroxide
    Ivan Spasojevic
    Center for Laser Microscopy, Faculty of Biology, University of Belgrade, Belgrade, Serbia
    Carbohydr Res 344:1676-81. 2009
    ....
  45. pmc Intracellular regulation of human ClC-5 by adenine nucleotides
    Giovanni Zifarelli
    Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Genova, Italy
    EMBO Rep 10:1111-6. 2009
    ..The residues Tyr 617 and Asp 727, but not Ser 618, are crucial for the potentiation. These results provide a mechanistic and structural framework for the interpretation of nucleotide regulation of a CLC transporter...
  46. ncbi Modeling and stochastic simulation of the Ras/cAMP/PKA pathway in the yeast Saccharomyces cerevisiae evidences a key regulatory function for intracellular guanine nucleotides pools
    Paolo Cazzaniga
    Universita degli Studi di Milano Bicocca, Dipartimento di Informatica, Sistemistica e Comunicazione, Viale Sarca 336, 20126 Milano, Italy
    J Biotechnol 133:377-85. 2008
    ..Finally, we show that the level of guanine nucleotides, GTP and GDP, could be relevant metabolic signals for the regulation of the whole pathway...
  47. ncbi Role of intracellular glutathione in cell sensitivity to the apoptosis induced by tumor necrosis factor {alpha}-related apoptosis-inducing ligand/anticancer drug combinations
    Olivier Meurette
    Institut National de la Santé et de la Recherche Médicale U620, Detoxication et Reparation Tissulaire, Faculte de Pharmacie, Universite Rennes 1, Rennes, France
    Clin Cancer Res 11:3075-83. 2005
    ..The present work further explores the role of intracellular reduced glutathione (GSH) level in cell sensitivity to this cell death pathway...
  48. ncbi Effects of SDF-1alpha and gp120IIIB on apoptotic pathways in SK-N-SH neuroblastoma cells
    Thomas Geeraerts
    Laboratoire Immunologie Antivirale Systémique et Cérébrale, INSERM EMI 0109, Faculte de Medecine Paris Sud, 63 rue Gabriel Peri, 94 276 Le Kremlin Bicêtre, France
    Neurosci Lett 399:115-20. 2006
    ..SDF-1alpha enhanced NMDA activity indirectly via Src phosphorylation, whereas gp120(IIIB) probably activated the NMDA receptor directly and phosphorylated JNK...
  49. pmc Structural determinants of Junctional Adhesion Molecule A (JAM-A) function and mechanisms of intracellular signaling
    Eric A Severson
    Department of Pathology and Laboratory Medicine, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Curr Opin Cell Biol 21:701-7. 2009
    ..Recent insights into JAM-A outside-in signaling events that regulate these cellular functions are discussed...
  50. ncbi Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines
    Dries Vercauteren
    Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    ACS Nano 5:7874-84. 2011
    ..Colocalization of polyplexes with the autophagy marker LC3 suggests for the first time that the process of xenophagy could play an important role in the persistent endosomal entrapment of nanomedicines...
  51. pmc Orai1 regulates intracellular calcium, arrest, and shape polarization during neutrophil recruitment in shear flow
    Ulrich Y Schaff
    Department of Biomedical Engineering, University of California, Davis, CA, USA
    Blood 115:657-66. 2010
    ..These results suggest that calcium entry via Orai1 is the predominant SOCE that cooperates with cytoplasmic calcium store release in coordinating integrin-dependent PMN arrest and migration in the acute response to inflammation...
  52. ncbi Intrabodies against the EVH1 domain of Wiskott-Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells
    Mitsuru Sato
    Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, Ibaraki, Japan
    FEBS J 272:6131-44. 2005
    ..The novel procedure presented here is a valuable tool for in vivo functional analysis of cytosolic proteins...
  53. ncbi pH-Triggered controlled drug release from mesoporous silica nanoparticles via intracelluar dissolution of ZnO nanolids
    Faheem Muhammad
    State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130021, China
    J Am Chem Soc 133:8778-81. 2011
    ..We anticipate that these nanoparticles may prove to be a significant step toward the development of a pH-sensitive drug delivery system that minimizes drug toxicity...
  54. ncbi When Helicobacter pylori invades and replicates in the cells
    Ya Hui Wang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Autophagy 5:540-2. 2009
    ..The replication of H. pylori in autophagic vesicles, and the consequences of this provide an important hint as to why this microorganism causes such a broad spectrum of diseases...
  55. ncbi Glutaredoxin-1 mediates NADPH-dependent stimulation of calcium-dependent insulin secretion
    Thomas M Reinbothe
    Department of Clinical Sciences, Islet Pathophysiology, Lund University, Clinical Research Centre, Malmo, Sweden
    Mol Endocrinol 23:893-900. 2009
    ..This effect is mediated by the NADPH acceptor protein GRX-1 by a local redox reaction that accelerates beta-cell exocytosis and, in turn, insulin secretion...
  56. pmc Cardiac overexpression of metallothionein rescues cardiac contractile dysfunction and endoplasmic reticulum stress but not autophagy in sepsis
    Asli F Ceylan-Isik
    Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, Laramie, WY 82071, USA
    J Mol Cell Cardiol 48:367-78. 2010
    ..LPS activated AMPK and its downstream signaling ACC in conjunction with an elevated AMP/ATP ratio, which was unaffected by MT. Taken together, our data favor a beneficial effect of MT in the management of cardiac dysfunction in sepsis...
  57. ncbi Hemocompatible pullulan-polyethyleneimine conjugates for liver cell gene delivery: In vitro evaluation of cellular uptake, intracellular trafficking and transfection efficiency
    M R Rekha
    Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Poojappura, Kerala, India
    Acta Biomater 7:370-9. 2011
    ..Therefore, the pullulan-PEI conjugate seems to be a promising gene delivery vector with good hemocompatibility and low toxicity but without compromising the transfection efficacy of PEI...
  58. ncbi Uptake kinetics of cell-penetrating peptides
    Anders Floren
    Laboratory of Molecular Biotechnology, Institute of Technology, Tartu University, Tartu, Estonia
    Methods Mol Biol 683:117-28. 2011
    ....
  59. ncbi Regulation of stem cell signaling by nanoparticle-mediated intracellular protein delivery
    Dhiral A Shah
    Howard P Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Biomaterials 32:3210-9. 2011
    ..This ability is critical for the design of in vitro screens for gain/loss of pathway function, and may also prove to be useful for in vivo delivery applications...
  60. pmc Acute-phase protein α1-antitrypsin inhibits neutrophil calpain I and induces random migration
    Mariam Al-Omari
    Department of Pulmonology, Hannover Medical School, Hannover, Germany
    Mol Med 17:865-74. 2011
    ....
  61. ncbi Active mechanical stabilization of the viscoplastic intracellular space of Dictyostelia cells by microtubule-actin crosstalk
    Doris Heinrich
    Physik Department, Lehrstuhl fuer Biophysik E22, Technische Universitaet Muenchen, D 85748 Garching, Germany
    Acta Biomater 2:619-31. 2006
    The micro-viscoelasticity of the intracellular space of Dictyostelium discoideum cells is studied by evaluating the intracellular transport of magnetic force probes and their viscoelastic responses to force pulses of 20-700 pN...
  62. ncbi iLoc-Virus: a multi-label learning classifier for identifying the subcellular localization of virus proteins with both single and multiple sites
    Xuan Xiao
    Computer Department, Jing De Zhen Ceramic Institute, Jing De Zhen, China
    J Theor Biol 284:42-51. 2011
    ..It is anticipated that iLoc-Virus may become a useful high throughput tool for both basic research and drug development...
  63. pmc Spatial chaos and complexity in the intracellular space of cancer and normal cells
    Tuan D Pham
    Aizu Research Cluster for Medical Engineering and Informatics, Center for Advanced Information Science and Technology, The University of Aizu, 965 8580, Aizuwakamatsu, Fukushima, Japan
    Theor Biol Med Model 10:62. 2013
    ..in biological image analysis is the quantification of the dynamical mechanism and complexity of the intracellular space. This paper investigates potential spatial chaos and complex behavior of the intracellular space of typical ..
  64. pmc Regulation of β2-adrenergic receptor maturation and anterograde trafficking by an interaction with Rab geranylgeranyltransferase: modulation of Rab geranylgeranylation by the receptor
    Veronik Lachance
    Service de Rhumatologie, Departement de Medecine, Faculte de Medecine et des Sciences de la Sante, Universite de Sherbrooke, the Centre de Recherche Clinique Étienne Le Bel, and the Institut de Pharmacologie de Sherbrooke, Sherbrooke, Quebec, Canada J1H 5N4
    J Biol Chem 286:40802-13. 2011
    ..We demonstrate for the first time an interaction between a transmembrane receptor and RGGTA which regulates the maturation and anterograde transport of the receptor, as well as geranylgeranylation of Rab GTPases...
  65. pmc The uptake and intracellular fate of PLGA nanoparticles in epithelial cells
    Malgorzata S Cartiera
    Department of Biomedical Engineering, Yale University, 55 Prospect Street, MEC 414, New Haven, CT 06520 8260, USA
    Biomaterials 30:2790-8. 2009
    ..We present a model for the intracellular fate of particles that is consistent with our experimental data...
  66. ncbi Intracellular cAMP: the "switch" that triggers on "spontaneous transient outward currents" generation in freshly isolated myocytes from thoracic aorta
    Sebastien Hayoz
    Department of Zoology and Animal Biology, University of Geneva, Sciences III, Geneva, Switzerland
    Am J Physiol Cell Physiol 292:C1502-9. 2007
    ..However, cellular machinery that generates STOCs can be "switched" on by cAMP. Such an inactive cellular mechanism could modulate the contractility of the thoracic aorta in response to physiological demand...
  67. pmc Massive comparative genomic analysis reveals convergent evolution of specialized bacteria
    Vicky Merhej
    Faculty of Medicine, Unit for Research on Emergent and Tropical Infectious Diseases, CNRS IRD UMR 6236 IFR48, University of the Mediterranean, Marseilles, France
    Biol Direct 4:13. 2009
    ..The growing number of available sequenced genomes makes it possible to perform a statistical comparative analysis of 317 genomes from bacteria with different lifestyles...
  68. ncbi Hydrogen peroxide depletes phosphatidylinositol-3-phosphate from endosomes in a p38 MAPK-dependent manner and perturbs endocytosis
    Fumi Kano
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
    Biochim Biophys Acta 1813:784-801. 2011
    ..This article is part of a Special Issue entitled: 11th European Symposium on Calcium...
  69. pmc Molecular dynamics investigation of Cl- and water transport through a eukaryotic CLC transporter
    Mary Hongying Cheng
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Biophys J 102:1363-71. 2012
    ..Finally, a conformational cycle is proposed that would account for the exchange stoichiometry...
  70. ncbi Sodium sensing in neurons with a dendrimer-based nanoprobe
    Christophe M Lamy
    Department of Cell Biology and Morphology, University of Lausanne, Rue du Bugnon 9, CH 1005 Lausanne, Switzerland
    ACS Nano 6:1176-87. 2012
    ..We demonstrate the suitability of this new sodium nanosensor for monitoring physiological sodium responses such as those occurring during neuronal activity...
  71. pmc Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway
    Tatyana Gurlo
    Larry Hillblom Islet Research Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095 7073, USA
    Am J Pathol 176:861-9. 2010
    ..Toxic oligomer-induced secretory pathway and mitochondrial membrane disruption is a novel mechanism to account for cellular dysfunction and apoptosis in T2DM...
  72. pmc Neurokinin-neurotrophin interactions in airway smooth muscle
    Lucas W Meuchel
    Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Am J Physiol Lung Cell Mol Physiol 301:L91-8. 2011
    ..The autocrine mechanism we present here represents a new area in the development of bronchoconstrictive reflex response and airway hyperreactive disorders...
  73. ncbi Markedly increased intracellular lipid droplets and disruption of intercellular junctions in biopsied myocardium from a patient with arrhythmogenic right ventricular cardiomyopathy
    Shuichi Fujita
    Department of Internal Medicine III, Osaka Medical College, 2 7 Daigaku machi, Takatsuki 569 8686, Japan
    Heart Vessels 23:440-4. 2008
    ....
  74. ncbi Biogenesis of outer membranes in Gram-negative bacteria
    Hajime Tokuda
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan
    Biosci Biotechnol Biochem 73:465-73. 2009
    ..In contrast, little is known about how phospholipids are transported to the outer membrane...
  75. ncbi Reaction kinetics in intracellular environments with macromolecular crowding: simulations and rate laws
    S Schnell
    Centre for Mathematical Biology, Mathematical Institute, Oxford, UK
    Prog Biophys Mol Biol 85:235-60. 2004
    ..We present a modification to fractal-like kinetics following the Zipf-Mandelbrot distribution which will enable the modelling and analysis of biochemical reactions occurring in crowded intracellular environments...
  76. pmc Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
    David P Dixon
    School of Biological and Biomedical Sciences, Durham University, Durham DH1 3LE, UK
    J Exp Bot 60:1207-18. 2009
    ..Based on the results obtained with the GSTTs, it was concluded that these proteins can exert identical protective functions in differing subcellular compartments...
  77. pmc Mucolipins: Intracellular TRPML1-3 channels
    Xiping Cheng
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    FEBS Lett 584:2013-21. 2010
    ..We will also discuss the potential cellular mechanisms by which TRPML deficiency leads to neurodegeneration...
  78. ncbi Truncation and pathogenic mutations facilitate the formation of intracellular aggregates of TDP-43
    Takashi Nonaka
    Department of Molecular Neurobiology, Tokyo Metropolitan Organization for Medical Research, Tokyo, Japan
    Hum Mol Genet 18:3353-64. 2009
    ....
  79. ncbi Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter
    Fredrik Lundh
    School of Pure and Applied Natural Sciences, Kalmar University, S 391 82 Kalmar, Sweden
    Biochemistry 48:4497-505. 2009
    ..Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown...
  80. ncbi Radish phospholipid hydroperoxide glutathione peroxidase provides protection against hydroperoxide-mediated injury in mouse 3T3 fibroblasts
    Tian Li
    Laboratory of Molecular Biology and MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China
    BMB Rep 42:648-54. 2009
    ..These results suggest the possibility for use of RsPHGPx as a therapeutic protectant...
  81. pmc Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling
    Masayuki J Sato
    Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
    Proc Natl Acad Sci U S A 106:6667-72. 2009
    ....
  82. ncbi Intracellular calcium regulation of connexin43
    Monica M Lurtz
    Department of Cell Biology and Neuroscience, University of California, Riverside, CA 92521, USA
    Am J Physiol Cell Physiol 293:C1806-13. 2007
    ..These data indicate that physiological concentrations of [Ca(2+)](i) regulate the permeability of Cx43 in a calmodulin-dependent manner that does not require the major portion of the COOH terminus of Cx43...
  83. pmc A tumor cord model for doxorubicin delivery and dose optimization in solid tumors
    Steffen Eikenberry
    Department of Mathematics and Statistics, Arizona State University, Tempe, AZ 85287, USA
    Theor Biol Med Model 6:16. 2009
    ..I model the delivery of a soluble drug from the vasculature to a solid tumor using a tumor cord model and examine the penetration of doxorubicin under different dosage regimes and tumor microenvironments...
  84. ncbi Uptake and intracellular fate of disulfide-bonded polymer hydrogel capsules for Doxorubicin delivery to colorectal cancer cells
    Yan Yan
    Department of Chemical and Biomolecular Engineering, The University of Melbourne, Centre for Nanoscience and Nanotechnology, Victoria 3010, Australia
    ACS Nano 4:2928-36. 2010
    ..The DOX-loaded PMA(SH) capsules demonstrate a 5000-fold enhanced cytotoxicity in cell viability studies compared to free DOX...
  85. ncbi Effects of quenched and annealed macromolecular crowding elements on a simple model for signaling in T lymphocytes
    Jonathan L Eide
    Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
    J Phys Chem B 110:2318-24. 2006
    ..We discuss these differences and their origin. Since many signaling reactions are fast, our results may be useful for diverse situations in cell biology...
  86. pmc Protein-protein interaction as a predictor of subcellular location
    Chang Jin Shin
    The University of Queensland, Institute for Molecular Bioscience, and ARC Centre of Excellence in Bioinformatics, QLD, Australia
    BMC Syst Biol 3:28. 2009
    ..Our goal here is to evaluate the potential of using PPI annotation in determining SCL of proteins in human, mouse, fly and yeast, and to identify and quantify the factors that contribute to this complementarity...
  87. ncbi Intracellular localization of homopolymeric amino acid-containing proteins expressed in mammalian cells
    Yoko Oma
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153 8902, Japan
    J Biol Chem 279:21217-22. 2004
    ..Our experiments provide novel insights into the nature of the HPAAs that are often seen in human and other organisms...
  88. pmc Prediction of disease-related mutations affecting protein localization
    Kirsti Laurila
    Institute of Medical Technology, University of Tampere, Tampere, Finland
    BMC Genomics 10:122. 2009
    ....
  89. pmc Proteome analysis of the penicillin producer Penicillium chrysogenum: characterization of protein changes during the industrial strain improvement
    Mohammad Saeid Jami
    Area de Microbiologia, Departamento de Biologia Molecular, Universidad de Leon, Campus de Vegazana s n, 24071 Leon, Spain
    Mol Cell Proteomics 9:1182-98. 2010
    ..These results provide useful information to improve the production of many other bioactive secondary metabolites...
  90. ncbi Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines
    Christos D Katsetos
    Department of Pediatrics, Drexel University College of Medicine, St Christopher s Hospital For Children, Philadelphia, Pennsylvania 19134, USA
    J Neuropathol Exp Neurol 65:465-77. 2006
    ....
  91. pmc Role of intracellular calcium and phospholipase A2 in arachidonic acid-induced toxicity in liver cells overexpressing CYP2E1
    Andres A Caro
    Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, Box 1603, One Gustave L Levy Place, New York, NY 10029, USA
    Arch Biochem Biophys 457:252-63. 2007
    ..These interactions may play a role in alcohol liver toxicity, which requires polyunsaturated fatty acids, and involves induction of CYP2E1...
  92. pmc Increased clusterin expression in Fuchs' endothelial dystrophy
    Ula V Jurkunas
    Schepens Eye Research Institute, Boston, Massachusetts, USA
    Invest Ophthalmol Vis Sci 49:2946-55. 2008
    ..To investigate the differential expression of the glycoprotein clusterin/apoJ (CLU) in normal and Fuchs' endothelial dystrophy (FED) corneal endothelium and to compare the expression of various forms of CLU in normal and FED tissue...
  93. pmc Control of extracellular cleavage of ProBDNF by high frequency neuronal activity
    Guhan Nagappan
    Section on Neural Development and Plasticity, National Institute of Child Health and Human Development, National Institutes of Health, 35 Lincoln Drive, Bethesda, MD 20892 3714, USA
    Proc Natl Acad Sci U S A 106:1267-72. 2009
    ..Our study demonstrates activity-dependent control of extracellular proteolytic cleavage of a secretory protein, and reveals an important mechanism that controls diametrically opposed functions of BDNF isoforms...
  94. pmc Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout
    Niina Sokolova
    Laboratory of Systems Biology, Institute of Cybernetics, Tallinn University of Technology, Akadeemia 21, 12618 Tallinn, Estonia
    BMC Cell Biol 10:90. 2009
    ..Experiments were performed at 10, 15 and 20 degrees C in the absence and presence of creatine...
  95. ncbi Enhanced antiproliferative activity of transferrin-conjugated paclitaxel-loaded nanoparticles is mediated via sustained intracellular drug retention
    Sanjeeb K Sahoo
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198 6025, USA
    Mol Pharm 2:373-83. 2005
    ....
  96. pmc Boosting accuracy of automated classification of fluorescence microscope images for location proteomics
    Kai Huang
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA
    BMC Bioinformatics 5:78. 2004
    ..Building on these results, we evaluate here new classifiers and features to improve the recognition of protein subcellular location patterns in both 2D and 3D fluorescence microscope images...
  97. pmc DBSubLoc: database of protein subcellular localization
    Tao Guo
    Institute of Bioinformatics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Nucleic Acids Res 32:D122-4. 2004
    ..The database now contains >60,000 protein sequences including approximately 30,000 protein sequences in the non-redundant data sets. Online download, search and Blast tools are also available...
  98. ncbi APECED-causing mutations in AIRE reveal the functional domains of the protein
    Maria Halonen
    Department of Molecular Medicine, National Public Health Institute, Helsinki, Finland
    Hum Mutat 23:245-57. 2004
    ....
  99. pmc Automatic identification of subcellular phenotypes on human cell arrays
    Christian Conrad
    Intelligent Bioinformatics Systems, German Cancer Research Center DKFZ, 69120 Heidelberg, Germany
    Genome Res 14:1130-6. 2004
    ..Our classification method can be adapted to virtually any microscopic assay based on cell morphology, opening a wide range of applications including large-scale RNAi screening in human cells...
  100. ncbi Intracellular distribution of a speech/language disorder associated FOXP2 mutant
    Akifumi Mizutani
    Division of Development, Department of Human Inherited Metabolic Disease, National Institute of Neuroscience, Ogawahigashi machi 4 1 1, Kodaira, Tokyo 187 8502, Japan
    Biochem Biophys Res Commun 353:869-74. 2007
    ..Mutant and wild type FOXP2 heterodimers in the nucleus or FOXP2 R553H in the cytoplasm may underlie the pathogenesis of the autosomal dominant speech/language disorder...
  101. ncbi Clearance of amyloid-beta peptide by neuronal and non-neuronal cells: proteolytic degradation by secreted and membrane associated proteases
    Mai Panchal
    Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, FRE 2621 CNRS, Universite Pierre et Marie Curie, Paris, France
    Curr Neurovasc Res 4:240-51. 2007
    ..This is the first time our results define a proteolytic degradation process of Abeta40 that appears to be independent of the cell type and may represent a general pattern of its enzymatic clearance...

Research Grants71

  1. Impact of neuronal chloride transport on treatment of seizures.
    Kevin J Staley; Fiscal Year: 2013
    ..cortical neurons, allowing the neurons to expand and accumulate the salt and water needed to fill the new intracellular space. One component of salt is chloride, and its accumulation causes the neurotransmitter GABA to promote rather ..
  2. Mapping pH at the surface of individual cell
    Yana K Reshetnyak; Fiscal Year: 2013
    ..the peptide inserting end (C-terminus), pHLIP would "flip" SNARF-1 across the bilayer and expose it to the intracellular space, while keeping it close to the inner leaflet of membrane...
  3. Estimating Intracellular Lithium in Brain in vivo by 7Li MRS
    Richard A Komoroski; Fiscal Year: 2010
    ..processes occur within the cell, it is often presumed that Li exerts its clinically relevant effects in the intracellular space of neurons in the brain. Intra- and extracellular Li cannot normally be distinguished by 7Li MRS...
  4. BLOOD BRAIN BARRIER LARGE NEUTRAL AMINO ACID TRANSPORTER
    William Pardridge; Fiscal Year: 2003
    ..surface area of the blood-brain barrier, the rate limiting step in amino acid movement from plasma to brain intracellular space is the BBB transport step...
  5. NUMERICAL SIMULATION OF CARDIAC ELECTROPHYSIOLOGY
    Bradley Roth; Fiscal Year: 2005
    ..However, near an epicardial electrode, current leaves the intracellular space to take advantage of the low resistance extracellular path (the high conductivity electrode material), ..
  6. BIOMEDICAL RESEARCH SUPPORT
    Michael Riley; Fiscal Year: 1992
    ..require detailed information on the three-dimensional localization of molecules in extracellular and intracellular space. Projects are described which involve applications of LSCM both to whole tissue samples and to cellular and ..
  7. VOLUME ABSORPTION IN THE RENAL PROXIMAL TUBULE
    James Williams; Fiscal Year: 1992
    ..the ability of the basement membrane to act as a semi-permeable membrane for abstraction of fluid from the intracellular space: and 5) To bring together the data that are collected into computer models that will: a) described the ..
  8. ENVIRONMENT OF LITHIUM IN RAT BRAIN IN VIVO
    Richard Komoroski; Fiscal Year: 2000
    ..There is little agreement as to how Li exerts its clinical effects, which are presumed to originate in the intracellular space of neurons...
  9. In Vivo Imaging of Retinal Apoptosis Using Activatable Permeation Peptides
    EDWARD BARNETT; Fiscal Year: 2007
    ..Access of molecular imaging probes to the intracellular space has been greatly improved through the use of permeation peptides as a targeting moiety...
  10. GH AND IGF-I TREATMENT OF ELDERLY WOMEN
    Andrew Hoffman; Fiscal Year: 2000
    ..previous studies which unexpectedly demonstrated that both hormones cause dramatic shifts of water into the intracellular space. Study III is a short term study that will examine the role of GH and IGF-1 in protein and lipid metabolism ..
  11. EXTRACELLULAR OXYGEN RADICALS AND ISCHEMIC BRAIN INJURY
    David Warner; Fiscal Year: 2002
    ..CuZnSOD and MnSOD have been shown to be important in scavenging O2- produced in the intracellular space. The remaining isozyme, extracellular SOD (EC-SOD), is found only in the extracellular (EC) space...
  12. Effects of intracellular angiotensin II
    Julia Cook; Fiscal Year: 2009
    ..abstract_text> ..
  13. Akt Inhibitors to Treat Ewing's Sarcoma
    Jeffrey Toretsky; Fiscal Year: 2005
    ..Our findings from this Phase I Sun will hopefully lead to a key compound that can be further developed in a Phase II STTR ultimately leading to clinical trials in patients with ESFT. ..
  14. The Glycosylation Gap and Diabetic Complications
    Robert Cohen; Fiscal Year: 2005
    ..The proposal therefore has potentially important basic science, clinical and population implications. ..
  15. IL-8 in chondrocalcinosis
    RU BRYAN; Fiscal Year: 2005
    ..The completion of this study has the potential to provide a foundation for novel chemokine and signal transduction-based therapeutic strategies for treatment of chondrocalcinosis in OA and aging. ..
  16. ANDROGEN PRODUCTION IN ADRENARCHE AND PCOS
    WALTER MILLER; Fiscal Year: 2006
    ..abstract_text> ..
  17. Postisoprenylation Processing and the Nuclear Lamina
    Stephen Young; Fiscal Year: 2007
    ..The third aim is to understand the posttranslational processing of lamin B1 and to define the consequences of lamin B1 deficiency in mammals. ..
  18. Mitoprotective and antioxidant peptides for DM treatment
    Hazel Szeto; Fiscal Year: 2006
    ..abstract_text> ..
  19. 3D Chromosome/Replisome Positioning in Bacterial Cells
    LUCILLE SHAPIRO; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  20. Songbird Neurogenomics Initiative
    David F Clayton; Fiscal Year: 2010
    ..Moreover, the approach here may serve as a model for doing science on a community scale, embracing individual initiative but coordinating efforts to achieve a larger synergy. ..
  21. 4.7 Tesla MRI Scanner Console and Gradient System
    Joseph Ackerman; Fiscal Year: 2005
    ..This proposal provides documentation of: need, past imaging performance, organizational and training competence, and institutional commitment. ..
  22. REQUEST TO UPGRADE 600 MHZ NMR SYSTEM
    Joseph Ackerman; Fiscal Year: 2001
    ..abstract_text> ..
  23. Conference on Signaling Via Cell-Cell Interactions
    Rudolph Juliano; Fiscal Year: 2003
    ..The proposed program will present a unique training opportunity for students and post-doctoral fellows, since it will provide a unifying thematic structure to converging, but still disparate, areas of research. ..
  24. Structure Function Studies Of Membrane Binding Proteins
    Wonhwa Cho; Fiscal Year: 2004
    ....
  25. ZINC NEUROTOXICITY
    Christian Sheline; Fiscal Year: 2004
    ....
  26. Analysis of fringe-dependent cell-signaling
    Kenneth Irvine; Fiscal Year: 2004
    ....
  27. Efficient Cellular Delivery of Oligonucleotides
    Charles M Roth; Fiscal Year: 2010
    ..Specifically, we will: (1) evaluate the effectiveness of our graft copolymers in several cell types with and without serum and (3) evaluate our delivery system in a tumor xenograft model. ..
  28. Innate Immunity in gouty Inflammation
    RU BRYAN; Fiscal Year: 2008
    ..Completion of this work will help further understanding the mechanisms of acute gouty inflammation, how to control gouty arthritis and potentially other forms of crystal-induced inflammation. [unreadable] [unreadable] [unreadable]..
  29. Molecular Mechanisms in Retinal Degeneration
    Debora B Farber; Fiscal Year: 2011
    ....
  30. Photothermal Imaging of Live Cells
    Vladimir Zharov; Fiscal Year: 2008
    ..The technical realization of PTI is relatively simple, requiring the combination of conventional optical phase-contrast microscopy and the already routinely used nanosecond laser. ..
  31. High Stability Accelerator for Sub-Cellular Microbeam
    David Brenner; Fiscal Year: 2003
    ..We have considerably more demand for microbeam time than we can cope with, primarily because of limited physicist manpower. ..
  32. EXPERIENCE DEPENDENT CHANGES IN THE BRAIN
    David Clayton; Fiscal Year: 2008
    ..Gold and Sweedler, we will test specific hypotheses using in vivo microdialysis, analytical chemistry and targeted injections of pharmacological agents. ..
  33. Novel GABA Actions on the Developing Brain
    Joseph Nunez; Fiscal Year: 2008
    ..I intend to pursue this research in a laboratory of my own. ..
  34. HIGH PRESSURE FREEZER
    LUCILLE SHAPIRO; Fiscal Year: 2008
    ..Each and every one of these projects demands a level of fixation that approaches the native, hydrated state which is possible only by application of high pressure freezing. [unreadable] [unreadable]..
  35. ENERGY METABOLISM, DOPAMINE NEURONS AND NEUROTOXICITY
    Gail Zeevalk; Fiscal Year: 2008
    ..The studies will have relevance to neurodegenerative conditions or pathophysiological situations in which metabolism is compromised. ..
  36. Class II Processing Delineated by Flu Glycoproteins
    Laurence Eisenlohr; Fiscal Year: 2008
    ..abstract_text> ..
  37. WASHINGTON UNIVERSITY SMALL ANIMAL IMAGING RESOURCE
    Joseph Ackerman; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  38. LIPOGENESIS & LIPOPROTEIN COMPLICATIONS OF HIV THERAPY
    David Y Hui; Fiscal Year: 2010
    ..Aim 3 will compare the functionality of the HDL after HAART therapy to determine if these are pro- or anti-atherogenic. ..
  39. IMMUNOBIOLOGY OF MHC RESTRICTION OF T CELLS
    Kenneth Rock; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  40. Chlamydial Manipulation of Host Apoptosis
    Guangming Zhong; Fiscal Year: 2010
    ..abstract_text> ..
  41. Calreticulin's functions in the adaptive immune response
    Malini Raghavan; Fiscal Year: 2010
    ..Understanding the molecular mechanisms of calreticulin's functions, and elucidation of conditions that enhance calreticulin's T cell priming activities, will facilitate more effective design of vaccines. ..
  42. Molecular Mechanisms of Natural Killer Cell Activation
    Subramaniam Malarkannan; Fiscal Year: 2010
    ..Mechanisms identified here will not only have practical implications in tumor and graft immunity but will serve as models for other systems in which the responding cell must balance a number of activating and inhibitory inputs. ..
  43. DEXASOME BASED IMMUNOTHERAPY OF LUNG CANCER
    Michael Morse; Fiscal Year: 2002
    ..This clinical trial will form the background for further trials designed to demonstrate both immunology and clinical benefits of dexosome-based immunotherapy. ..
  44. Molecular mechanism of SIF formation by Salmonella Typhimurium
    Trina Schroer; Fiscal Year: 2008
    ..A comprehensive understanding of the molecular mechanisms that underlie this process is necessary to identify novel targets for therapy and disease prevention. ..
  45. Vaccination with Regulatory T Cell Depletion
    Michael Morse; Fiscal Year: 2007
    ..Future studies will assess whether the greater magnitude and durability of the CEA-specific immune responses will translate into a long-term clinical benefit. [unreadable] [unreadable] [unreadable]..
  46. Hyperacidification and Pseudomonas infections in CF
    Vojo Deretic; Fiscal Year: 2006
    ..abstract_text> ..
  47. Immunobiology of Antigen Presenting Cells in Vivo
    Kenneth Rock; Fiscal Year: 2006
    ..The goals of this Aim are to test these hypotheses and determine the underlying mechanism for the loss APCs. ..
  48. MOLECULAR BIOLOGY OF TRANSALDOLASE
    Andras Perl; Fiscal Year: 2004
    ..The proposed studies with the availability of unique reagents and existing model systems will further our knowledge on the role of TAL in the PPP, apoptosis signaling, and related disease processes. ..
  49. REGULATION OF SPERM EXOCYTOSIS
    Nicholas Cross; Fiscal Year: 2002
    ..The cholesterol content of freshly ejaculated and oviductal bovine sperm will be compared to see if sperm lose cholesterol in the female tract. ..
  50. INTERLEUKIN 2 IMMUNOTHERAPY FOR HCV INFECTION
    KENDALL SMITH; Fiscal Year: 2004
    ..This approach should prove beneficial in improving the proportion of patients who recover from HCV infection, and as well, introduce immunologic tests that may be predictive of responsiveness to therapy. ..
  51. Magnex Model MRBR 7T/201 AS Shielded Magnet System
    Jean Philippe Galons; Fiscal Year: 2005
    ..7 Tesla platform. Therefore, the need to acquire new imaging capabilities and the growth of the UoA MRI imaging community are the main justification for the present request. ..
  52. Integrated optical imaging rat of mesentery
    Vladimir Zharov; Fiscal Year: 2004
    ..The technical realization of this new imaging system is relatively simple, combining transmission microscopy and laser technique already routinely in use. ..
  53. Cell-Permeable Peptides for Mitochondrial Protection
    Hazel Szeto; Fiscal Year: 2005
    ..Potential collaborators for the animal studies have already been identified. ..
  54. Cleveland Cell Biology Symposium: Regulation
    ALAN TARTAKOFF; Fiscal Year: 2004
    ..The results should be an intense synthesis of knowledge which will be stimulating for both students and established faculty. ..
  55. Efficient and Selective Delivery of Oligonucleotides
    Charles Roth; Fiscal Year: 2006
    ..W expect the long-term outcome to be a selective and efficient method for oligonucleotide delivery for therapeutic and functional genomics applications. ..
  56. MYCOBACTERIAL ENVIRONMENT WITHIN MACROPHAGES
    Luiz Bermudez; Fiscal Year: 2003
    ..We believe that the proposed studies will generate important information about the phagosome environment and the mycobacterial response to it. ..
  57. Major Challenges in Clinical Medicine: An Overview for *
    Rudolph Juliano; Fiscal Year: 2006
    ..As part of this component the trainee, with the mentor's guidance, will prepare the Abstract and Specific Aims of an NIH-type grant proposal dealing with a translational research topic. ..
  58. PHOSPHODIESTERASES IN PHOTORECEPTOR METABOLISM & DISEASE
    Debora Farber; Fiscal Year: 2003
    ..Overall, these studies are designed to increase our knowledge of the etiology and possibly the pathogenesis of certain hereditary retinal degenerations and open new avenues for future therapeutic modalities. ..
  59. The Role of the RET Receptor in Autoimmune Disease
    Laurence Eisenlohr; Fiscal Year: 2007
    ..By deciphering the role of RETECD antibody in disease, we can design improved methods and drugs for the abrogation of thyroid and other organ-specific autoimmune disorders. [unreadable] [unreadable] [unreadable]..
  60. Exploring Growth Energy Expenditure in Normal-Weight and Overweight Children
    ZiMian Wang; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  61. 2008 Genes and Behavior Gordon Research Conference
    David Clayton; Fiscal Year: 2007
    ..This conference will promote progress towards this understanding by bringing together experts working with a variety of organismal models, human populations and technical approaches. [unreadable] [unreadable] [unreadable]..
  62. Farnesol Analogues as Novel Treatment of Alcoholism
    Jean Baptiste Roullet; Fiscal Year: 2005
    ..Potential commercialization: This research will provide the foundation for Phase II evaluation of the most active farnesol analogue in the treatment of alcohol withdrawal syndrome in alcoholic patients. ..
  63. System Upgrade for Small-Animal MRI
    Joseph Ackerman; Fiscal Year: 2007
    ..Magnetic resonance imaging, the subject of this proposal, allows a safe, non- invasive, non-destructive means to evaluate these models and translate findings to patients. [unreadable] [unreadable] [unreadable]..
  64. Proteomic charaterization of insulin signaling targets
    JONATHAN BOGAN; Fiscal Year: 2006
    ..Additionally, this work may have broader implications for insulin signaling and for protein targeting. [unreadable] [unreadable]..
  65. CCD Camera for Intermediate Voltage Electron Microscopes
    Kenneth H Downing; Fiscal Year: 2010
    ..These adaptations will produce substantial benefits in work ranging from high-resolution structural studies of proteins and viruses to the three dimensional study of cell ultrastructure. ..
  66. Ca2+ Sparks as Regulators of Airway Contractility
    Ronghua Zhuge; Fiscal Year: 2007
    ..We expect that these studies will provide new knowledge which could lead to development of novel therapeutic approaches for asthma and other bronchospasitc disorders. ..
  67. Cryopreservation of the model organism Drosophila melanogaster
    BRENT SINCLAIR; Fiscal Year: 2007
    ..The ultimate goal is to cryopreserve D. melanogaster, an achievement that will greatly increase the utility of the Drosophila model for human disease research. ..
  68. Cholesterol and brain injury-induced Abeta
    Mark Burns; Fiscal Year: 2008
    ..As such this project has multidisciplinary benefits and is relevant to the fields of CNS cholesterol, Alzheimer's disease and brain injury. [unreadable] [unreadable] [unreadable]..
  69. Novel Cationic 99mTc Complexes for Heart Imaging
    Shuang Liu; Fiscal Year: 2006
    ..Successful development of new 99mTc perfusion imaging agents will have a profound impact on diagnostic evaluation, risk stratification, and therapeutic decision-making in patients with CAD. ..
  70. CALCIUM SIGNALING IN AIRWAY EPITHELIA AND SMOOTH MUSCLE
    Michael Sanderson; Fiscal Year: 2002
    ..With this understanding our ability to design therapies to counter obstructive lung disease and prevent asthmatic attacks will be enhanced. ..