intracellular membranes

Summary

Summary: Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.

Top Publications

  1. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
  2. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
  3. pmc Role of PINK1 binding to the TOM complex and alternate intracellular membranes in recruitment and activation of the E3 ligase Parkin
    Michael Lazarou
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 22:320-33. 2012
  4. pmc Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
    N Mizushima
    Unit Process and Combined Circuit, PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Biol 152:657-68. 2001
  5. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
  6. ncbi Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses
    R Rizzuto
    Department of Biomedical Sciences and the National Research Council Center for the Study of Biomembranes, University of Padova, Via Colombo 3, 35121 Padova, Italy
    Science 280:1763-6. 1998
  7. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
  8. ncbi Modification of intracellular membrane structures for virus replication
    Sven Miller
    3 V Biosciences, Institute of Biochemistry, Schafmattstrasse 18, ETH Hoenggerberg, HPME 17, CH8093 Zurich, Switzerland
    Nat Rev Microbiol 6:363-74. 2008
  9. ncbi Autophagy: process and function
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Genes Dev 21:2861-73. 2007
  10. pmc The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    M Babst
    Division of Cellular Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine La Jolla, CA 92093 0668, USA
    EMBO J 17:2982-93. 1998

Research Grants

Detail Information

Publications311 found, 100 shown here

  1. pmc LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Y Kabeya
    Department of Cell Biology, National Institute for Basic Biology, Okazaki 444 8585, PRESTO, Japan
    EMBO J 19:5720-8. 2000
    ..The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes...
  2. pmc Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
    Elizabeth L Axe
    Signalling Programme, Babraham Institute, Babraham, Cambridge, England, UK
    J Cell Biol 182:685-701. 2008
    ..This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation...
  3. pmc Role of PINK1 binding to the TOM complex and alternate intracellular membranes in recruitment and activation of the E3 ligase Parkin
    Michael Lazarou
    Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 22:320-33. 2012
    ..We propose that the association of PINK1 with the TOM complex allows rapid reimport of PINK1 to rescue repolarized mitochondria from mitophagy, and discount mitochondrial-specific factors for Parkin translocation and activation...
  4. pmc Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells
    N Mizushima
    Unit Process and Combined Circuit, PRESTO, Japan Science and Technology Corporation, Kawaguchi 332 0012, Japan
    J Cell Biol 152:657-68. 2001
    ..We also show that Apg12-Apg5 is required for targeting of a mammalian Aut7/Apg8 homologue, LC3, to the isolation membranes. These results suggest that the Apg12-Apg5 conjugate plays essential roles in isolation membrane development...
  5. ncbi Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked
    J Yang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Science 275:1129-32. 1997
    ..Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria...
  6. ncbi Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses
    R Rizzuto
    Department of Biomedical Sciences and the National Research Council Center for the Study of Biomembranes, University of Padova, Via Colombo 3, 35121 Padova, Italy
    Science 280:1763-6. 1998
    ..These results emphasize the importance of cell architecture and the distribution of organelles in regulation of Ca2+ signaling...
  7. ncbi Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC
    S Shimizu
    Osaka University Medical School, Biomedical Research Center, Department of Medical Genetics, Suita, Japan
    Nature 399:483-7. 1999
    ..Our results indicate that the Bcl-2 family of proteins bind to the VDAC in order to regulate the mitochondrial membrane potential and the release of cytochrome c during apoptosis...
  8. ncbi Modification of intracellular membrane structures for virus replication
    Sven Miller
    3 V Biosciences, Institute of Biochemistry, Schafmattstrasse 18, ETH Hoenggerberg, HPME 17, CH8093 Zurich, Switzerland
    Nat Rev Microbiol 6:363-74. 2008
    ..We describe how viruses modify intracellular membranes, highlight similarities between the structures that are induced by viruses of different families and ..
  9. ncbi Autophagy: process and function
    Noboru Mizushima
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113 8519, Japan
    Genes Dev 21:2861-73. 2007
    ..In this review, the process of autophagy is summarized, and the role of autophagy is discussed in a process-based manner...
  10. pmc The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function
    M Babst
    Division of Cellular Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine La Jolla, CA 92093 0668, USA
    EMBO J 17:2982-93. 1998
    ..Together, the data suggest that the Vps4 ATPase catalyzes the release (uncoating) of an endosomal membrane-associated class E protein complex(es) required for normal morphology and sorting activity of the endosome...
  11. ncbi Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  12. ncbi Mechanisms shaping the membranes of cellular organelles
    Yoko Shibata
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 25:329-54. 2009
    ..Mechanisms affecting local membrane curvature may also shape peripheral ER sheets and the nuclear envelope as well as mitochondria and caveolae...
  13. pmc Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death
    M C Wei
    Howard Hughes Medical Institute, Departments of Pathology and Medicine, Harvard Medical School, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 292:727-30. 2001
    ..Thus, activation of a "multidomain" proapoptotic member, BAX or BAK, appears to be an essential gateway to mitochondrial dysfunction required for cell death in response to diverse stimuli...
  14. ncbi Bax and adenine nucleotide translocator cooperate in the mitochondrial control of apoptosis
    I Marzo
    CNRS, UPR 420, 19 rue Guy Moquet, F 94801 Villejuif, France
    Science 281:2027-31. 1998
    ..Hence, the proapoptotic molecule Bax and the constitutive mitochondrial protein ANT cooperate within the PTPC to increase mitochondrial membrane permeability and to trigger cell death...
  15. ncbi The ubiquitylation machinery of the endoplasmic reticulum
    Christian Hirsch
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13125 Berlin, Germany
    Nature 458:453-60. 2009
    ..The importance of this pathway is evident from the growing list of pathologies associated with quality-control defects in the ER...
  16. pmc Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae
    Aniek van der Vaart
    Department of Cell Biology and Institute of Biomembranes, University Medical Center Utrecht, Utrecht 3584, The Netherlands
    Mol Biol Cell 21:2270-84. 2010
    ..Our data show that the Golgi complex plays a crucial role in supplying the lipid bilayers necessary for the biogenesis of double-membrane vesicles possibly through a new class of transport carriers or a new mechanism...
  17. ncbi Bax is present as a high molecular weight oligomer/complex in the mitochondrial membrane of apoptotic cells
    B Antonsson
    Serono Pharmaceutical Research Institute, Serono International S A, 14 Chemin des Aulx, CH 1228 Plan les Ouates, Geneva, Switzerland
    J Biol Chem 276:11615-23. 2001
    ..Bax oligomerization appears to be required for its proapoptotic activity, and the Bax oligomer/complex might constitute the structural entirety of the cytochrome c-conducting channel in the outer mitochondrial membrane...
  18. ncbi The Vam6 GEF controls TORC1 by activating the EGO complex
    Matteo Binda
    Department of Medicine, University of Fribourg, Switzerland
    Mol Cell 35:563-73. 2009
    ..Thus, in addition to its regulatory role in homotypic vacuolar fusion and vacuole protein sorting within the HOPS complex, Vam6 also controls TORC1 function by activating the Gtr1 subunit of the EGO complex...
  19. pmc Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response
    Sebastian Schuck
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 187:525-36. 2009
    ..Thus, increasing ER size through membrane synthesis is an integral yet distinct part of the cellular program to overcome ER stress...
  20. pmc Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy
    A Orsi
    London Research Institute, Cancer Research UK, London, United Kingdom
    Mol Biol Cell 23:1860-73. 2012
    ....
  21. ncbi A photoactivatable GFP for selective photolabeling of proteins and cells
    George H Patterson
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Science 297:1873-7. 2002
    ..Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange...
  22. ncbi A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function
    H Horiuchi
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 90:1149-59. 1997
    ..This demonstrates that a soluble exchange factor coupled to a Rab effector translocates from cytosol to the membrane, where the complex stabilizes the GTPase in the active state...
  23. pmc Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape
    H Sesaki
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 147:699-706. 1999
    ..Our results thus suggest that mitochondrial shape is normally controlled by a balance between division and fusion which requires Dnm1p and Fzo1p, respectively...
  24. pmc Evidence for a two membrane-spanning autonomous mitochondrial DNA replisome
    Shelly Meeusen
    Section of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA
    J Cell Biol 163:503-10. 2003
    ..Taken together, our observations reveal the existence of an autonomous two membrane-spanning mitochondrial replisome as well as provide a mechanism for how mtDNA replication and inheritance may be physically linked...
  25. ncbi Mitochondria and neuronal survival
    D G Nicholls
    Department of Pharmacology, University of Dundee, Dundee, Scotland
    Physiol Rev 80:315-60. 2000
    ..Mitochondria play an integral role in both necrotic and apoptotic neuronal cell death, and the bioenergetic principles underlying current studies are reviewed...
  26. ncbi The mitochondrion in apoptosis: how Pandora's box opens
    N Zamzami
    Centre National de la Recherche Scientifique, UMR1599, Institut Gustave Roussy, 39 rue Camille-Desmoulins, F-94805 Villejuif, France
    Nat Rev Mol Cell Biol 2:67-71. 2001
    ..Here we suggest that opening of a multiprotein complex called the mitochondrial permeability transition pore complex is sufficient (and, usually, necessary) for triggering apoptosis...
  27. pmc Toxoplasma gondii Tic20 is essential for apicoplast protein import
    Giel G van Dooren
    Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 105:13574-9. 2008
    ..Our studies provide experimental evidence for a common evolutionary origin of import mechanisms across the innermost membranes of primary and secondary plastids...
  28. ncbi Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase
    H P Harding
    Skirball Institute of Biomolecular Medicine, Department of Medicine, NYU School of Medicine, New York 10016, USA
    Nature 397:271-4. 1999
    ..These properties implicate PERK in a signalling pathway that attenuates protein translation in response to ER stress...
  29. pmc The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast
    W Bleazard
    Department of Biology, University of Utah, Salt Lake City 84112, USA
    Nat Cell Biol 1:298-304. 1999
    ..These findings indicate that Dnm1 regulates mitochondrial fission, assembling on the cytoplasmic face of mitochondrial tubules at sites at which division will occur...
  30. pmc Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane
    R Schneiter
    Spezialforschungsbereich Biomembrane Research Center, Institut fur Biochemie und Lebensmittelchemie, Technische Universitat Graz, A 8010 Graz, Austria
    J Cell Biol 146:741-54. 1999
    ..This observation suggests that acyl chain-based sorting and/or remodeling mechanisms are operating to maintain the specific lipid molecular species composition of the yeast plasma membrane...
  31. ncbi Compartmental specificity of cellular membrane fusion encoded in SNARE proteins
    J A McNew
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 407:153-9. 2000
    ..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis...
  32. ncbi Vesicle tethering complexes in membrane traffic
    James R C Whyte
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    J Cell Sci 115:2627-37. 2002
    ..Moreover, the diversity of tethering complexes in the cell suggests that not all tethering events occur through the same mechanisms...
  33. pmc SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum
    Kevin Knoops
    Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    PLoS Biol 6:e226. 2008
    ..Together with biochemical studies of the viral enzyme complex, our ultrastructural description of this "replication network" will aid to further dissect the early stages of the coronavirus life cycle and its virus-host interactions...
  34. ncbi Live imaging of yeast Golgi cisternal maturation
    Kumi Matsuura-Tokita
    Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Wako, Saitama 351 0198, Japan
    Nature 441:1007-10. 2006
    ....
  35. ncbi Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria
    G Daum
    J Biol Chem 257:13028-33. 1982
    ..The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria...
  36. ncbi Mitochondrial control of cell death
    G Kroemer
    Centre National de la Recherche Scientifique, CNRS ULR1599, Institut Gustave Roussy 24 rue Calmette Desmoulins, F 94805 Villejuif
    Nat Med 6:513-9. 2000
    ..In many instances, permeabilization of mitochondrial membranes is a rate-limiting step of apoptotic or necrotic cell demise. This has important consequences for the pathophysiology of cell death, as well as for its pharmacological control...
  37. pmc PEX19 is a predominantly cytosolic chaperone and import receptor for class 1 peroxisomal membrane proteins
    Jacob M Jones
    Dept of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    J Cell Biol 164:57-67. 2004
    ....
  38. pmc Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability
    A E Hobbs
    Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Cell Biol 152:401-10. 2001
    ..We propose that Mmm1p is part of a connection between the mitochondrial outer and inner membranes, anchoring mitochondrial DNA nucleoids in the matrix...
  39. ncbi Double identity for proteins of the Bcl-2 family
    J C Reed
    Burnham Institute, Program on Apoptosis and Cell Death Research, La Jolla, California 92037, USA
    Nature 387:773-6. 1997
    ..The mechanisms used by this and related anti-apoptotic proteins to protect cells from cytotoxic stimuli are now emerging, with the discovery that Bcl-2 can function both as an ion channel and as an adaptor or docking protein...
  40. pmc HOPS initiates vacuole docking by tethering membranes before trans-SNARE complex assembly
    Christopher M Hickey
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Mol Biol Cell 21:2297-305. 2010
    ..SNAREs further stabilize the associations of HOPS-tethered membranes. HOPS then protects newly formed trans-SNARE complexes from disassembly by Sec17p/Sec18p...
  41. pmc Control systems for membrane fusion in the ancestral eukaryote; evolution of tethering complexes and SM proteins
    V Lila Koumandou
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK
    BMC Evol Biol 7:29. 2007
    ..This pattern is also likely for Rabs and various other components of the membrane trafficking machinery...
  42. ncbi Functional architecture of an intracellular membrane t-SNARE
    R Fukuda
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 407:198-202. 2000
    ..Although there are numerous homologues of syntaxin on intracellular membranes, there are only two SNAP-25-related proteins in yeast, Sec9 and Spo20, both of which are localized to the ..
  43. ncbi Breaking the mitochondrial barrier
    J C Martinou
    Departement de Biologie Cellulaire, Sciences III, 30 Quai Ernest Ansermet, 1211 Geneve 4, Switzerland
    Nat Rev Mol Cell Biol 2:63-7. 2001
    ..They could do this either by forming autonomous pores in the membrane or by collaborating with components of the permeability transition pore. Here we discuss why we favour the first of these possibilities...
  44. ncbi Mechanisms of membrane deformation
    Khashayar Farsad
    Department of Cell Biology, Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA
    Curr Opin Cell Biol 15:372-81. 2003
    ..A variety of proteins have been identified that directly bind and deform membranes. An emerging theme in this process is the importance of amphipathic peptides that partially penetrate the lipid bilayer...
  45. pmc Membrane delivery to the yeast autophagosome from the Golgi-endosomal system
    Yohei Ohashi
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, UK
    Mol Biol Cell 21:3998-4008. 2010
    ..This is underestimated by examination of single mutants, providing a possible explanation for discrepancies between yeast and mammalian studies on Atg9 localization and autophagosome formation...
  46. ncbi C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane
    A M Labrousse
    Department of Biological Chemistry, University of California, Los Angeles School of Medicine 90095, USA
    Mol Cell 4:815-26. 1999
    ..These data indicate that wild-type DRP-1 contributes to the final stages of mitochondrial division by controlling scission of the mitochondrial outer membrane...
  47. ncbi Coat proteins and vesicle budding
    R Schekman
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720 3202, USA
    Science 271:1526-33. 1996
    ..Secretion, which has been viewed as a default pathway, may require sorting and packaging signals on transported molecules to ensure their rapid delivery to the cell surface...
  48. pmc Complex dynamic development of poliovirus membranous replication complexes
    George A Belov
    Virginia Maryland College of Veterinary Medicine, University of Maryland, College Park, Maryland 20742, USA
    J Virol 86:302-12. 2012
    ..Thus, it is likely that there is a fundamental divergence in the requirements for the supporting cellular membrane-shaping machinery among different groups of positive-strand RNA viruses...
  49. ncbi The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis
    S Frank
    Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Behesda, Maryland 20892, USA
    Dev Cell 1:515-25. 2001
    ..Remarkably, inhibition of Drp1 blocks cell death, implicating mitochondrial fission as an important step in apoptosis...
  50. pmc The ATP synthase is involved in generating mitochondrial cristae morphology
    Patrick Paumard
    Institut de Biochimie et Genetique Cellulaires du CNRS, Universite Victor Segalen, Bordeaux 2, 1 rue Camille Saint Saens, 33077 Bordeaux Cedex, France
    EMBO J 21:221-30. 2002
    ....
  51. ncbi Protein translocation across the ER membrane
    Richard Zimmermann
    Medical Biochemistry and Molecular Biology, Saarland University, D 66041 Homburg, Germany
    Biochim Biophys Acta 1808:912-24. 2011
    ..This article is part of a Special Issue entitled Protein translocation across or insertion into membranes...
  52. ncbi Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation
    N W Bays
    Section of Cell and Developmental Biology, Division of Biology, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093 0347, USA
    Nat Cell Biol 3:24-9. 2001
    ..In this capacity, Hrd1p has an apparent preference for misfolded proteins. We also show that Hrd1p functions as an E3 in vivo, using only Ubc7p or Ubc1p to specifically program the ubiquitination of ERAD substrates...
  53. pmc Pre- and post-Golgi translocation of glucosylceramide in glycosphingolipid synthesis
    David Halter
    Membrane Enzymology, Bijvoet Center, and Department of Cell Biology, University Medical Center Utrecht, Institute of Biomembranes, Utrecht University, 3584 Utrecht, Netherlands
    J Cell Biol 179:101-15. 2007
    ..FAPP2 knockdown strongly reduces GM3 synthesis. Overall, we show that newly synthesized GlcCer enters two pathways: one toward the noncytosolic surface of a post-Golgi membrane and one via the ER toward the Golgi lumen LacCer synthase...
  54. ncbi EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  55. ncbi 3D tomography reveals connections between the phagophore and endoplasmic reticulum
    Päivi Ylä-Anttila
    University of Helsinki, Department of Biological and Environmental Sciences, Division of Biochemistry, Helsinki, Finland
    Autophagy 5:1180-5. 2009
    ..This finding has potential implications on the origin of autophagosome membranes, and on the mechanism of phagophore membrane extension. In addition, we observed lipid droplets in very close contact with the phagophores/autophagosomes...
  56. ncbi Disruption of the outer mitochondrial membrane as a result of large amplitude swelling: the impact of irreversible permeability transition
    P X Petit
    CNRS UPR420, Génétique Moléculaire et Biologie du Développement 19, Villejuif, France
    FEBS Lett 426:111-6. 1998
    ..Moreover, they confirm the hypothesis formulated by Skulachev [FEBS Lett. 397 (1996) 7-10 and Q. Rev. Biophys. 29 (1996) 169-2021 linking permeability transition to activation of the apoptogenic catabolic enzymes...
  57. pmc Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae
    K Kuchler
    J Bacteriol 165:901-10. 1986
    ..Data concerning susceptibility to various inhibitors, heat sensitivity, and the pH optima indicate that there is a close similarity of the mitochondrial and microsomal enzymes that catalyze the same reaction...
  58. ncbi Oligomeric complexes link Rab5 effectors with NSF and drive membrane fusion via interactions between EEA1 and syntaxin 13
    H M McBride
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 98:377-86. 1999
    ..We propose a novel mechanism whereby oligomeric EEA1 and NSF mediate the local activation of syntaxin 13 upon membrane tethering and, by analogy with viral fusion proteins, coordinate the assembly of a fusion pore...
  59. pmc Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ADP-ribosylation factors in membrane traffic between the trans-Golgi network and endosomes
    Ray Ishizaki
    Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606 8501, Japan
    Mol Biol Cell 19:2650-60. 2008
    ..Taken together with previous reports, these observations indicate that BIG2 and BIG1 play redundant roles in trafficking between the TGN and endosomes that involves the AP-1 complex...
  60. ncbi Bcl-xL regulates the membrane potential and volume homeostasis of mitochondria
    M G Vander Heiden
    Gwen Knapp Center and Committee on Immunology, Department of Medicine, University of Chicago, Illinois 60637, USA
    Cell 91:627-37. 1997
    ..These data suggest that Bcl-xL promotes cell survival by regulating the electrical and osmotic homeostasis of mitochondria...
  61. pmc A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery
    Istvan R Boldogh
    Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA
    Mol Biol Cell 14:4618-27. 2003
    ....
  62. pmc Membrane curvature during peroxisome fission requires Pex11
    Łukasz Opaliński
    Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute GBB, University of Groningen, Kluyver Centre for Genomics of Industrial Fermentation, Haren, The Netherlands
    EMBO J 30:5-16. 2011
    ..Finally, we demonstrate that mutations abolishing the membrane remodelling activity of the Pex11-Amph domain also hamper the function of full-length Pex11 in peroxisome fission in vivo...
  63. ncbi Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion
    H Stenmark
    European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany
    Cell 83:423-32. 1995
    ..Immunodepletion of rabaptin-5 from cytosol strongly inhibits Rab5-dependent early endosome fusion. Rabaptin-5 is thus a Rab effector required for membrane docking and fusion...
  64. pmc A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes
    Robert Yung Liang Wang
    Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546, USA
    J Virol 83:3276-87. 2009
    ....
  65. ncbi Protein transport into chloroplasts
    Hsou Min Li
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan
    Annu Rev Plant Biol 61:157-80. 2010
    ..The functions or expression levels of some translocon components are further adjusted according to plastid type, developmental stage, and metabolic conditions...
  66. pmc FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells
    Taichi Hara
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Bunkyo ku, Tokyo 113 8549, Japan
    J Cell Biol 181:497-510. 2008
    ..These results suggest that FIP200 is a novel mammalian autophagy factor that functions together with ULKs...
  67. pmc TRP channels of intracellular membranes
    Xian Ping Dong
    The Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Neurochem 113:313-28. 2010
    ..Ion channels serve a number of functions in intracellular membranes as well...
  68. pmc Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes
    Natalie Luhtala
    Molecular, Cellular, and Developmental Biology, 347 UCB, University of Colorado, Boulder, CO 80309 0347, USA
    J Cell Biol 166:717-29. 2004
    ..Thus, our results point to a key role for Bro1 in coordinating the timing and location of deubiquitination by Doa4 in the MVB pathway...
  69. ncbi Drosophila glucosylceramide synthase: a negative regulator of cell death mediated by proapoptotic factors
    Ayako Kohyama-Koganeya
    Neuronal Circuit Mechanisms Research Group, Brain Science Institute, The Institute of Physical and Chemical Research RIKEN, Wako Shi, Saitama 351 0198, Japan
    J Biol Chem 279:35995-6002. 2004
    ..These results indicate that GlcT-1 might down-regulate ceramide generated in these membranes...
  70. pmc GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking
    Yi Xiang
    Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 188:237-51. 2010
    ..These results demonstrate that GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism...
  71. pmc Irradiation-induced protein inactivation reveals Golgi enzyme cycling to cell periphery
    Timothy Jarvela
    Department of Biological Sciences, Carnegie Mellon University, 4400 5th Avenue, Pittsburgh, PA 15213, USA
    J Cell Sci 125:973-80. 2012
    ..These experiments demonstrate that ER exit and extensive cycling of cis-Golgi components to the cell periphery sustain the mammalian Golgi complex...
  72. pmc Der3p/Hrd1p is required for endoplasmic reticulum-associated degradation of misfolded lumenal and integral membrane proteins
    J Bordallo
    Institut fur Biochemie, Universitat Stuttgart, Germany
    Mol Biol Cell 9:209-22. 1998
    ..Der3p might serve as a component programming the translocon for retrograde transport of ER proteins, or it might be involved in recognition through its lumenal RING-H2 motif of proteins of the ER that are destined for degradation...
  73. pmc Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells
    David L Marks
    Thoracic Diseases Research Unit, Department of Medicine, Mayo Clinic College of Medicine, Stabile 8, 200 First Street, SW, Rochester, MN 55905 0001, USA
    Histochem Cell Biol 130:819-32. 2008
    ....
  74. pmc A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi
    A L Boman
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
    Mol Biol Cell 11:1241-55. 2000
    ....
  75. pmc Regulation by salt of vacuolar H+-ATPase and H+-pyrophosphatase activities and Na+/H+ exchange
    Paulo Silva
    Centro de Investigação e de Tecnologias Agro Ambientais e Biológicas, Portugal
    Plant Signal Behav 4:718-26. 2009
    ..In this review we focus on the transcriptional and post-transcriptional regulation by salt of tonoplast proton pumps and Na(+)/H(+) exchangers and on the signalling pathways involved in salt sensing...
  76. ncbi Mutated human SOD1 causes dysfunction of oxidative phosphorylation in mitochondria of transgenic mice
    Marina Mattiazzi
    Department of Neurology and Neuroscience, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Biol Chem 277:29626-33. 2002
    ..Our findings suggest that G93A-mutated hSOD1 in mitochondria may cause mitochondrial defects, which contribute to precipitating the neurodegenerative process in motor neurons...
  77. ncbi Structure of human estrone sulfatase suggests functional roles of membrane association
    Francisco G Hernandez-Guzman
    Department of Structural Biology, Hauptman Woodward Medical Research Institute, Buffalo, New York 14203, USA
    J Biol Chem 278:22989-97. 2003
    ..This simple architecture could be a prototype utilized by the ER membrane in dictating the form and the function of ER-resident enzymes...
  78. ncbi Fission and proliferation of peroxisomes
    M Schrader
    Department of Biology, University of Aveiro, Aveiro, Portugal
    Biochim Biophys Acta 1822:1343-57. 2012
    ..Finally, we present an updated model of peroxisomal growth and division. This article is part of a Special Issue entitled: Metabolic Functions and Biogenesis of Peroxisomes in Health and Disease...
  79. ncbi The yeast deubiquitinating enzyme Ubp16 is anchored to the outer mitochondrial membrane
    Andrea Kinner
    Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Geb 26 12 01, Universitatsstr 1, D 40225 Dusseldorf, Germany
    FEBS Lett 549:135-40. 2003
    ....
  80. pmc Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1
    Lydie Michaillat
    Departement de Biochimie, Universite de Lausanne, 1066 Epalinges, Switzerland
    Mol Biol Cell 23:881-95. 2012
    ....
  81. pmc The C terminus of Bax inhibitor-1 forms a Ca2+-permeable channel pore
    Geert Bultynck
    Department of Molecular Cell Biology, Laboratory of Molecular and Cellular Signaling, K U Leuven, B 3000 Leuven, Belgium
    J Biol Chem 287:2544-57. 2012
    ..Remarkably, one of these residues was not conserved among plant and yeast BI-1 orthologs, indicating that the ER Ca(2+)-leak properties of BI-1 are an added function during evolution...
  82. pmc Ultrastructural characterization of arterivirus replication structures: reshaping the endoplasmic reticulum to accommodate viral RNA synthesis
    Kevin Knoops
    Electron Microscopy Section, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    J Virol 86:2474-87. 2012
    ..This potential intermediate in nucleocapsid formation, which was not observed in coronavirus-infected cells, suggests that arterivirus RNA synthesis and assembly are coordinated in intracellular space...
  83. pmc Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p
    X Pan
    Department of Biology, University of Rochester, Rochester NY, 14627, USA
    Mol Biol Cell 11:2445-57. 2000
    ..Overexpression of Nvj1p caused the profound proliferation of NV junctions. We conclude that Vac8p and Nvj1p are necessary components of a novel interorganelle junction apparatus...
  84. pmc Infectious HIV-1 assembles in late endosomes in primary macrophages
    Annegret Pelchen-Matthews
    Cell Biology Unit, Medical Research MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
    J Cell Biol 162:443-55. 2003
    ..This notion has significant implications for understanding the biology of HIV and its cell-cell transmission...
  85. pmc Eeyarestatin 1 interferes with both retrograde and anterograde intracellular trafficking pathways
    Mina Olga Aletrari
    School of Life Sciences, University of Warwick, Coventry, United Kingdom
    PLoS ONE 6:e22713. 2011
    ..We therefore investigated ESI as a potential inhibitor of toxin dislocation...
  86. pmc The ERAD inhibitor Eeyarestatin I is a bifunctional compound with a membrane-binding domain and a p97/VCP inhibitory group
    Qiuyan Wang
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 5:e15479. 2010
    ..We recently reported that the ERAD inhibitor Eeyarestatin I (EerI) also disturbs ER homeostasis and has anti-cancer activities resembling that of Bortezomib...
  87. pmc Modification of a ubiquitin-like protein Paz2 conducted micropexophagy through formation of a novel membrane structure
    Hiroyuki Mukaiyama
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
    Mol Biol Cell 15:58-70. 2004
    ..We herein show that Paz2 and its modification system conduct micropexophagy through formation of the membrane structure, which explains the convergence between micropexophagy and macroautophagy with regard to de novo membrane formation...
  88. pmc Studies on the mechanisms of autophagy: formation of the autophagic vacuole
    W A Dunn
    Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville 32610
    J Cell Biol 110:1923-33. 1990
    ..The results were consistent with the formation of autophagic vacuoles from ribosome-free regions of the RER...
  89. pmc AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins
    Myriam Ferro
    INSERM, Laboratoire d Etude de Dynamique des Protéomes, U880, France
    Mol Cell Proteomics 9:1063-84. 2010
    ..It also allowed revisiting the compartmentation of the chloroplast metabolism and functions...
  90. ncbi Spontaneous cell polarization through actomyosin-based delivery of the Cdc42 GTPase
    Roland Wedlich-Soldner
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Science 299:1231-5. 2003
    ....
  91. pmc Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria
    Martin Kolisek
    Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr Bohrgasse, A 1030 Vienna, Austria
    EMBO J 22:1235-44. 2003
    ..Taken together, these findings characterize Mrs2p as the first molecularly identified metal ion channel protein in the inner mitochondrial membrane...
  92. ncbi Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopy
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, INSERM ERI 19, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours Cedex, France
    Histochem Cell Biol 130:561-6. 2008
    ..This may have important implications for our understanding of the complex relationship between HCV and lipids and may make easier to dissect out the HCV life cycle...
  93. ncbi The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome
    Karin Nowikovsky
    Max F Perutz Laboratories, Departments of Microbiology and Genetics, University of Vienna, Campus Vienna Biocenter, A 1030 Vienna, Austria
    J Biol Chem 279:30307-15. 2004
    ..Thus, YOL027/LETM1 are the first genes shown to encode factors involved in both K+ homeostasis and organelle volume control...
  94. ncbi Structural basis of mitochondrial tethering by mitofusin complexes
    Takumi Koshiba
    Division of Biology, California Institute of Technology, 1200 East California Boulevard, MC114 96, Pasadena, CA 91125, USA
    Science 305:858-62. 2004
    ..Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion...
  95. pmc Three v-SNAREs and two t-SNAREs, present in a pentameric cis-SNARE complex on isolated vacuoles, are essential for homotypic fusion
    C Ungermann
    Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755 3844, USA
    J Cell Biol 145:1435-42. 1999
    ..Our data show that vacuole-vacuole fusion requires a cis-SNARE complex of five SNAREs, the t-SNAREs Vam3p and Vam7p and the v-SNAREs Nyv1p, Vti1p, and Ykt6p...
  96. pmc Identification and functional reconstitution of the yeast peroxisomal adenine nucleotide transporter
    L Palmieri
    Department of Pharmaco Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via E Orabona 4, 70125 Bari, Italy
    EMBO J 20:5049-59. 2001
    ..The physiological role of Ant1p in S.cerevisiae is probably to transport cytoplasmic ATP into the peroxisomal lumen in exchange for AMP generated in the activation of fatty acids...
  97. ncbi Exosomes derived from IL-10-treated dendritic cells can suppress inflammation and collagen-induced arthritis
    Seon Hee Kim
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Immunol 174:6440-8. 2005
    ..Thus DC-derived exosomes may represent a novel, cell-free therapy for the treatment of autoimmune diseases...
  98. pmc ER membrane-bending proteins are necessary for de novo nuclear pore formation
    T Renee Dawson
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232, USA
    J Cell Biol 184:659-75. 2009
    ..We hypothesize that these ER membrane-bending proteins mediate early NPC assembly steps...
  99. ncbi Bcl-2 prevents Bax oligomerization in the mitochondrial outer membrane
    V Mikhailov
    Department of Pathology, University of Texas Health Science Center, San Antonio, Texas 78229, USA
    J Biol Chem 276:18361-74. 2001
    ....
  100. pmc The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission
    Quinton Tieu
    Section of Molecular and Cellular Biology, University of California, Davis, 95616, USA
    J Cell Biol 158:445-52. 2002
    ....
  101. ncbi Tubulin is an inherent component of mitochondrial membranes that interacts with the voltage-dependent anion channel
    Manon Carre
    UMR CNRS 6032, UFR Pharmacy, University of La Méditerranée, 27 Boulevard Jean Moulin, 13005 Marseille, France
    J Biol Chem 277:33664-9. 2002
    ..Thus, tubulin is an inherent component of mitochondrial membranes, and it could play a role in apoptosis via interaction with the permeability transition pore...

Research Grants62

  1. Regulation of K-Ras by a Farnesyl-electrostatic Switch
    MARK REID PHILIPS; Fiscal Year: 2013
    ..switch that resulted in release of K-Ras from the plasma membrane and association with intracellular membranes, including the endoplasmic reticulum (ER), Golgi apparatus and the outer mitochondrial membrane...
  2. MOLECULAR PROPERTIES OF IONIC PERMEABILITY IN NERVE
    Bertil Hille; Fiscal Year: 2013
    ..The focus includes intracellular membranes and compartments as well as the plasma membrane...
  3. Activation of PI Specific Phospholipase C Enzymes
    MARY FEDARKO ROBERTS; Fiscal Year: 2013
    ..Insight into how this PI-PLC, which contributes to virulence, interacts with intracellular membranes may provide alternate ways of slowing intracellular growth of this organism...
  4. Role of Protein Kinase C in Macrophage Activation
    Michelle R Lennartz; Fiscal Year: 2012
    ..Phagocytosis requires the focal delivery of intracellular membranes to the site of target binding. How this occurs and what directs that focal delivery is unknown...
  5. Assembly dynamics and role of PI4P enriched replication organelles for enterovira
    Ann Cali; Fiscal Year: 2013
    ..provided by applicant): Cells infected with plus-strand RNA viruses undergo dramatic remodeling of their intracellular membranes into so-called replication organelles...
  6. Reconstitution of Poliovirus and HCV RNA Replication Complexes on Membranes
    JENNIFER BARBARA PTACEK; Fiscal Year: 2010
    ..The tethering of viral RNA replication complexes to the cytosolic surface of intracellular membranes could affect several aspects of their structure and specific activity...
  7. Autophagy in COPD
    George R Washko; Fiscal Year: 2010
    ..Generally, the process of autophagy involves rearrangement of intracellular membranes to sequester a specifically selected "cargo" (including damaged or degraded proteins or organelles) ..
  8. Heme Oxygenase-1/Carbon Monoxide in Lung Vascular Injury
    Augustine M Choi; Fiscal Year: 2013
    ..manifested by degradation of cytoplasmic organelles via a lysosomal pathway, involving rearrangement of intracellular membranes to sequester damaged proteins or organelles within formed membrane vesicles, or autophagosomes...
  9. Apoptosis Control by Bcl-2 Family Members
    Craig B Thompson; Fiscal Year: 2012
    ..All Bcl-2 family members have been shown to localize to intracellular membranes where they contribute to the regulation of membrane integrity and/or the modulation of membrane ..
  10. Subversion of Autophagy Pathway and Constituents by RNA viruses
    Karla Kirkegaard; Fiscal Year: 2013
    ..provided by applicant): Positive-strand RNA viruses that infect eukaryotic cells all rearrange intracellular membranes and replicate their RNA genomes on the topologically cytoplasmic surfaces...
  11. TRAINING PROGRAM IN INTEGRATIVE MEMBRANE BIOLOGY
    Scott M Thompson; Fiscal Year: 2013
    ..th University of Maryland School of Medicine (UM SOM), by focusing on the role of the cell membrane and intracellular membranes in mediating and integrating the functions of cells and their interactions with the environment...
  12. EndoPulse System for Endoscopic Ultrasound-Guided Therapy of Pancreatic Carcinoma
    RICHARD LEE NUCCITELLI; Fiscal Year: 2010
    ..The mechanism by which nsPEF causes tumor regression involves increasing the permeability of intracellular membranes and triggering apoptosis...
  13. TUG-mediated GLUT4 glucose transporter trafficking and regulation by post-transla
    JONATHAN PHILIP BELMAN; Fiscal Year: 2012
    ..Translocation of GLUT4 from intracellular membranes to the cell surface is required for insulin to enhance glucose uptake, and defects in this process in ..
  14. The Role of CLIC4 in Angiogenesis
    John C Edwards; Fiscal Year: 2013
    ..CLIC proteins are a family of chloride channel proteins which largely reside in intracellular membranes. Chloride permeability has long been recognized to play a role in intracellular membrane traffic but the ..
  15. Intracellular delivery of proapoptotic peptide drugs for the treatment of cancer
    Patrick S Stayton; Fiscal Year: 2013
    ..facilitating tumor targeting, circulation stability, and translocation of proapoptotic peptides across intracellular membranes will augment their anti- tumor potency and substantially enhance their effectiveness...
  16. INTRACELLULAR TRANSPORT OF AMPHIPATHS
    Bruce Luxon; Fiscal Year: 2000
    ..transport rate of these fluorescent probes as well as their partition between soluble proteins and intracellular membranes. These measurements will be done in rat hepatocytes and hepatocyte couplets as well as in human hepatoma ..
  17. MECHANISMS UNDERLYING INTRACELLULAR MGLUR5 ROLE IN NOCICEPTION
    KAREN L OMALLEY; Fiscal Year: 2013
    ..in any neuronal type including dorsal horn neurons, despite studies showing 60-90% of the receptor is on intracellular membranes. Thus a clearer understanding of the specific role played by spinal intracellular mGluR5 in chronic ..
  18. Bar Domains and Neuronal Membrane Structure
    Tobias Meyer; Fiscal Year: 2013
    ..Nevertheless, it is difficult from these assays to know to which curved intracellular membranes these proteins may bind, and if or how they act dynamically to generate distinct types of curved plasma ..
  19. BRAIN PHOSPHOINOSITIDES AND BARBITURATES
    DIWAKAR DESHMUKH; Fiscal Year: 1993
    ..of synaptosomes or synaptoneurosomes by cholinergic muscarinic agonists, and of platelets by thrombin will be measured as diminished release of Ca from the intracellular membranes, followed fluorimetrically as Ca-fura-2 formation.
  20. Genetics and Functions of HSV Membrane Fusion Proteins
    KONSTANTIN KOUSOULAS; Fiscal Year: 2009
    ..process, in which interactions between tegument proteins and viral glycoproteins embedded in intracellular membranes play important roles...
  21. Molecular Analysis of Coronavirus Assembly
    BRENDA HOGUE; Fiscal Year: 2007
    ..In Aim 1 we will focus on understanding the role of the E and M proteins in targeting assembly to intracellular membranes. We will precisely identify the site of intracellular localization for E...
  22. GLUCOSE-DEPENDENT GLUT-1 EXPRESSION IN EMBRYONIC HEART
    IDA WASHINGTON; Fiscal Year: 2003
    ..is expected to decrease glucose uptake and phosphorylation acutely and cause Glut-1 translocation to intracellular membranes after intermediate exposure and decreased Glut-1 mRNA after chronic exposure...
  23. Intracellular Assembly of the Coronavirus, IBV
    Carolyn Machamer; Fiscal Year: 2005
    ..Assembly of enveloped viruses at intracellular membranes is less well understood, although these viruses must also accumulate their membrane proteins at the ..
  24. REGULATION & FUNCTION OF CALCIUM TRANSPORTING ATPASES
    GARY SHULL; Fiscal Year: 1993
    Calcium transporting ATPases of the plasma membrane and intracellular membranes plays a key role in the extrusion of Ca+2 from the cell and its sequestration within intracellular organelles...
  25. MOLECULAR REGULATION OF INTESTINAL AMINOOLIGOPEPTIDASE
    Mitchell Cohen; Fiscal Year: 1991
    ..this oligopeptide induces a doubling in the incorporation of [3H]-L-leucine into the AOP associated with intracellular membranes. The molecular events controlling both the developmental expression of this enzyme during ontogeny, as ..
  26. SIGNIFICANCE OF MEMBRANE PHOSPHOLIPID ASYMMETRY
    Bertram Lubin; Fiscal Year: 1999
    ..PL homeostasis in plasma and intracellular membranes of reticulocytes will be studied by using transferrin receptor based methods to separate the plasma ..
  27. Virus-host interactions in replication complex assembly
    David Miller; Fiscal Year: 2009
    ..the varied clinical syndromes caused by these viruses, all characterized positive-strand RNA viruses use intracellular membranes for viral RNA replication complex formation and function...
  28. MOLECULAR FUNCTION OF SYNUCLEIN
    JULIA GEORGE; Fiscal Year: 2004
    ..function is related to its conserved structural features, which allow it to bind reversibly with intracellular membranes. Manipulation of these interactions could have uses in the development of therapies for age-related ..
  29. ROLE OF ARGININE TRANSPORT IN NITRIC OXIDE PRODUCTION
    JAMES CUNNINGHAM; Fiscal Year: 2001
    ..In recent experiments, they have observed that MCAT-2B resides exclusively on intracellular membranes, likely lysosomes, a location distinct from MCAT-1 and MCAT-2A...
  30. Regulation of PIP2 Metabolism in Nerve Terminals
    Belle Chang; Fiscal Year: 2009
    ..The phospholipid PIP2 is found to be enriched on the plasma membrane and at significantly lower levels in intracellular membranes. This proposal strives to study the mechanisms controlling the spatial restriction of PIP2 ..
  31. STRUCTURE AND FUNCTION OF THE CORONAVIRUS REPLICASE
    Susan Baker; Fiscal Year: 2005
    ..In this proposal, we will investigate how the replicase polyprotein is processed, assembled on intracellular membranes, and functions in viral RNA synthesis...
  32. STRUCTURE FUNCTION ANALYSIS OF ENERGY TRANSDUCTION
    Michael Douglas; Fiscal Year: 1991
    The generation of intracellular proton gradients across different intracellular membranes is necessary for the function of these membranes in a variety of cellular events...
  33. TUMOR LIPIDS AND HYPERTHERMIA/CHEMOTHERAPY
    CHARLES BURNS; Fiscal Year: 1992
    ..biochemical (lipid composition) and physical results (membrane order) of the substitution on plasma and intracellular membranes will be delineated. Studies on both a murine and human neoplasm are included...
  34. MEMBRANE LIPID-ASSOCIATED CHANGES DURING AGING
    ARLENE MARTIN; Fiscal Year: 1990
    ..Specifically we will focus on: Lipid Composition of Intracellular Membranes 1...
  35. Mechanism of HCV Internalization into Target Cells
    Tatjana Dragic; Fiscal Year: 2006
    ..a co-receptor (CD81), which leads to internalization of viral particles and low pH-dependent fusion with intracellular membranes. Surprisingly, we discovered that HCV envelope glycoproteins cannot induce receptor-mediated, low pH-..
  36. Regulation of Mammalian Phospholipases A2
    Nicole Okeley; Fiscal Year: 2003
    ..We propose to study the molecular basis of the selective targeting of c PLA2 to intracellular membranes and to study its regulation by phosphorylation in keratinocytes...
  37. Targeting Apolipoprotein E4-related Neuropathology
    Robert Mahley; Fiscal Year: 2004
    ..ApoE4 destabilization of intracellular membranes represents one mechanism whereby apoE4 could be related to neuropathology...
  38. Biochemistry of Herpes Simplex Tegument Components
    Duncan Wilson; Fiscal Year: 2005
    ..Next, we will dissect the molecular mechanism by which vhs binds to intracellular membranes. Finally, we have successfully isolated primary enveloped HSV particles from the perinuclear space...
  39. MITOCHONDRIA AND APOPTOSIS OF MAMMARY EPITHELIAL CELLS
    Barbara Heerdt; Fiscal Year: 2001
    ..abstract_text> ..
  40. Role of Mammalian PITP Beta in Neurodegenerative Disease
    VYTAS BANKAITIS; Fiscal Year: 2005
    ..The Bankaitis laboratory is in a unique position to undertake this line of research as it has developed the requisite genetic and biochemical systems for such comprehensive analyses. ..
  41. PTDINS TRANSFER PROTEIN FUNCTION AND NEURODEGENERATION
    VYTAS BANKAITIS; Fiscal Year: 2007
    ..The Bankaitis laboratory is uniquely poised to address questions of mechanism of PITPalpha function as it has developed unique experimental systems for analysis. ..
  42. Gordon Conference-Autophagy/Stress/Development/Disease
    Ana Cuervo; Fiscal Year: 2007
    ....
  43. PROTEOMIC ANALYSIS OF DECREASED AUTOPHAGY IN AGING
    Ana Cuervo; Fiscal Year: 2005
    ..abstract_text> ..
  44. Short chain fatty acid metabolism and colonic tumorigenesis
    Barbara Heerdt; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  45. Colon Cancer and Mitochondria
    Barbara Heerdt; Fiscal Year: 2006
    ..abstract_text> ..
  46. POSTTRANSLATIONAL PROTEIN TRANSPORT INTO YEAST ER
    Tom Rapoport; Fiscal Year: 2003
    ..It will test the hypothesis that this protein works as a molecular ratchet rather than a machine that "pulls" the protein through the membrane. ..
  47. Molecular analysis of the dimeric F1/F0-ATP synthase
    Rosemary Stuart; Fiscal Year: 2006
    ....
  48. Aging-Related Models and Mitochondrial Bioenergetics
    David Nicholls; Fiscal Year: 2006
    ..3. To indicate therapeutic strategies whereby the deleterious consequences of aging-related changes in mitochondrial function can be minimized. ..
  49. MOLECULAR TOXICOLOGY IN HUMAN KIDNEY CELLS
    Lawrence Lash; Fiscal Year: 2007
    ..Achievement of these aims should build on our previous work in human kidney cells and extend it to provide a much more complete understanding of the various and complex ways in which DCVC affects the human kidney [unreadable] [unreadable]..
  50. Mitochondrial control of neuronal excitotoxicity
    David Nicholls; Fiscal Year: 2005
    ..4. To test the hypothesis that mitochondria at specific intracellular locations may be selectively vulnerable to glutamate excitotoxicity because of their proximity to NMDA receptors, or their location in regions of high-energy demand. ..
  51. Role of BCL-xL in synaptic plasticity in the hippocampus
    Elizabeth Ann Jonas; Fiscal Year: 2010
    ..We hypothesize that these changes are required for long term changes in synaptic strength after high frequency synaptic stimulation, therefore we plan to test whether inhibition of BClxl prevents l TP. ..
  52. COMPARTMENTAL ORGANIZATION OF THE YEAST GOLGI COMPLEX
    TODD GRAHAM; Fiscal Year: 2002
    ..In addition, loss of specific Golgi enzyme activities lead to several serious human disorders including I- cell disease, pseudo-Hurler polydystrophy, HEMPAS disease and Lowe syndrome. ..
  53. GLUTATHIONE AND MITOCHONDRIA IN TOXIC RENAL INJURY
    Lawrence Lash; Fiscal Year: 2003
    ..This information may have therapeutic applications for prevention of renal cellular injury or for understanding mitochondrial diseases or age-related decreases that occur in mitochondrial function. ..
  54. Phospholipid and Arachidonic Acid Signaling in Epilepsy
    Nicolas Bazan; Fiscal Year: 2007
    ..abstract_text> ..
  55. Proteomic charaterization of insulin signaling targets
    JONATHAN BOGAN; Fiscal Year: 2006
    ..Additionally, this work may have broader implications for insulin signaling and for protein targeting. [unreadable] [unreadable]..
  56. Chloride Channel Function & Role in Cardiovas. Disease
    JOSEPH HUME; Fiscal Year: 2009
    ..For a pilot project aimed to identify/increase expression of chloride channel ClC-3 in the heart, to be of therapeutic value to Duchenne Muscular Dystrophy patients. ..
  57. EGF, Oxidants & Intestinal Barrier Integrity
    Ali Banan; Fiscal Year: 2006
    ..abstract_text> ..
  58. Role of Selenoprotein P in Male Fertility
    Gary Olson; Fiscal Year: 2007
    ..unreadable] Aim 3: To identify and localize Se-P-binding proteins in the testis and epididymis. [unreadable] Aim 4: To determine the molecular basis for sperm defects in Se-P null mice. [unreadable] [unreadable]..
  59. SUBUNIT ASSEMBLY DOMAINS--TARGETS TO LIMIT NEUROTOXICITY
    Scott Belcher; Fiscal Year: 2002
    ..abstract_text> ..
  60. IONIC AND PHARMACOLOGICAL REGULATION OF VASCULAR CELLS
    JOSEPH HUME; Fiscal Year: 2007
    ..unreadable]..