nuclear lamina

Summary

Summary: A lattice of fibrils which covers the entire inner surface of the nuclear envelope and interlinks nuclear pores (NUCLEAR PORE).

Top Publications

  1. ncbi Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation
    Daan Peric-Hupkes
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Cell 38:603-13. 2010
  2. ncbi Regions of focal DNA hypermethylation and long-range hypomethylation in colorectal cancer coincide with nuclear lamina-associated domains
    Benjamin P Berman
    University of Southern California Epigenome Center, University of Southern California, Keck School of Medicine, Los Angeles, California, USA
    Nat Genet 44:40-6. 2012
  3. ncbi DNA sequence-dependent compartmentalization and silencing of chromatin at the nuclear lamina
    Joseph M Zullo
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Cell 149:1474-87. 2012
  4. pmc The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription
    Takeshi Shimi
    Department of Cell and Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611, USA
    Genes Dev 22:3409-21. 2008
  5. ncbi Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
    Lars Guelen
    Division of Molecular Biology, Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands
    Nature 453:948-51. 2008
  6. pmc Nuclear lamina defects cause ATM-dependent NF-κB activation and link accelerated aging to a systemic inflammatory response
    Fernando G Osorio
    Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, Oviedo, Spain
    Genes Dev 26:2311-24. 2012
  7. pmc Lamin B1 is required for mouse development and nuclear integrity
    Laurent Vergnes
    Veterans Affairs Greater Los Angeles Healthcare System, David Geffen School of Medicine, University of California, Los Angeles, CA 90073, USA
    Proc Natl Acad Sci U S A 101:10428-33. 2004
  8. pmc Diseases of the nuclear envelope
    Howard J Worman
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Cold Spring Harb Perspect Biol 2:a000760. 2010
  9. pmc Constitutive nuclear lamina-genome interactions are highly conserved and associated with A/T-rich sequence
    Wouter Meuleman
    Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Genome Res 23:270-80. 2013
  10. ncbi Single-cell dynamics of genome-nuclear lamina interactions
    Jop Kind
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, 1066 CX, The Netherlands
    Cell 153:178-92. 2013

Detail Information

Publications243 found, 100 shown here

  1. ncbi Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation
    Daan Peric-Hupkes
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    Mol Cell 38:603-13. 2010
    ..To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural ..
  2. ncbi Regions of focal DNA hypermethylation and long-range hypomethylation in colorectal cancer coincide with nuclear lamina-associated domains
    Benjamin P Berman
    University of Southern California Epigenome Center, University of Southern California, Keck School of Medicine, Los Angeles, California, USA
    Nat Genet 44:40-6. 2012
    ..domains covered nearly half of the genome and coincided with late replication and attachment to the nuclear lamina in human cell lines...
  3. ncbi DNA sequence-dependent compartmentalization and silencing of chromatin at the nuclear lamina
    Joseph M Zullo
    Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA
    Cell 149:1474-87. 2012
    A large fraction of the mammalian genome is organized into inactive chromosomal domains along the nuclear lamina. The mechanism by which these lamina associated domains (LADs) are established remains to be elucidated...
  4. pmc The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription
    Takeshi Shimi
    Department of Cell and Molecular Biology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611, USA
    Genes Dev 22:3409-21. 2008
    ..Our evidence suggests that the organization and regulation of chromatin are influenced by interconnections between these lamin microdomains...
  5. ncbi Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
    Lars Guelen
    Division of Molecular Biology, Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, The Netherlands
    Nature 453:948-51. 2008
    ..Microscopy studies have indicated that specific regions of chromosomes are located in close proximity to the nuclear lamina (NL)...
  6. pmc Nuclear lamina defects cause ATM-dependent NF-κB activation and link accelerated aging to a systemic inflammatory response
    Fernando G Osorio
    Departamento de Bioquimica y Biologia Molecular, Instituto Universitario de Oncologia, Universidad de Oviedo, Oviedo, Spain
    Genes Dev 26:2311-24. 2012
    Alterations in the architecture and dynamics of the nuclear lamina have a causal role in normal and accelerated aging through both cell-autonomous and systemic mechanisms...
  7. pmc Lamin B1 is required for mouse development and nuclear integrity
    Laurent Vergnes
    Veterans Affairs Greater Los Angeles Healthcare System, David Geffen School of Medicine, University of California, Los Angeles, CA 90073, USA
    Proc Natl Acad Sci U S A 101:10428-33. 2004
    Lamins are key structural components of the nuclear lamina, an intermediate filament meshwork that lies beneath the inner nuclear membrane. Lamins play a role in nuclear architecture, DNA replication, and gene expression...
  8. pmc Diseases of the nuclear envelope
    Howard J Worman
    Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Cold Spring Harb Perspect Biol 2:a000760. 2010
    ..diseases are also caused by mutations in genes encoding B-type lamins and proteins that associate with the nuclear lamina. Studies of these so-called laminopathies or nuclear envelopathies, some of which phenocopy common human ..
  9. pmc Constitutive nuclear lamina-genome interactions are highly conserved and associated with A/T-rich sequence
    Wouter Meuleman
    Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Genome Res 23:270-80. 2013
    In metazoans, the nuclear lamina is thought to play an important role in the spatial organization of interphase chromosomes, by providing anchoring sites for large genomic segments named lamina-associated domains (LADs)...
  10. ncbi Single-cell dynamics of genome-nuclear lamina interactions
    Jop Kind
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, 1066 CX, The Netherlands
    Cell 153:178-92. 2013
    The nuclear lamina (NL) interacts with hundreds of large genomic regions termed lamina associated domains (LADs). The dynamics of these interactions and the relation to epigenetic modifications are poorly understood...
  11. pmc Distinct structural and mechanical properties of the nuclear lamina in Hutchinson-Gilford progeria syndrome
    Kris Noel Dahl
    Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 103:10271-6. 2006
    The nuclear lamina is a network of structural filaments, the A and B type lamins, located at the nuclear envelope and throughout the nucleus...
  12. pmc Nuclear lamina at the crossroads of the cytoplasm and nucleus
    Larry Gerace
    Department of Cell, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA
    J Struct Biol 177:24-31. 2012
    The nuclear lamina is a protein meshwork that lines the nuclear envelope in metazoan cells...
  13. pmc The defective nuclear lamina in Hutchinson-gilford progeria syndrome disrupts the nucleocytoplasmic Ran gradient and inhibits nuclear localization of Ubc9
    Joshua B Kelley
    Center for Cell Signaling, University of Virginia, Charlottesville, Virginia, USA
    Mol Cell Biol 31:3378-95. 2011
    ..progeria syndrome (termed progerin) acts as a dominant negative protein that changes the structure of the nuclear lamina. How the perturbation of the nuclear lamina in progeria is transduced into cellular changes is undefined...
  14. ncbi The nuclear lamina as a gene-silencing hub
    Yuri Y Shevelyov
    Department of Molecular Genetics of Cell, Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia
    Curr Issues Mol Biol 14:27-38. 2012
    ..The integrity of the nuclear lamina and the histone deacetylase activity appear to be essential for gene repression at the nuclear periphery...
  15. pmc The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome
    Joke G van Bemmel
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 5:e15013. 2010
    Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression...
  16. ncbi Role of the nuclear lamina in genome organization and gene expression
    D Peric-Hupkes
    Division of Gene Regulation, Netherlands Cancer Institute, 1066 CX, Amsterdam, The Netherlands
    Cold Spring Harb Symp Quant Biol 75:517-24. 2010
    The nuclear lamina is a major structural component of metazoan nuclei that has long been thought to provide an anchoring site for interphase chromosomes and have a role in gene regulation...
  17. pmc Robust nuclear lamina-based cell classification of aging and senescent cells
    Christiaan H Righolt
    Department of Human Genetics, Leiden University Medical Center, The Netherlands
    Aging (Albany NY) 3:1192-201. 2011
    Changes in the shape of the nuclear lamina are exhibited in senescent cells, as well as in cells expressing mutations in lamina genes...
  18. ncbi Laminopathies
    Jos L V Broers
    Department of Molecular Cell Biology, Research Institutes CARIM, GROW, and EURON, University of Maastricht, The Netherlands
    J Pathol 204:478-88. 2004
    ..Which of these identities underlies the laminopathies, a myriad of genetic diseases caused by mutations in lamins or lamin-associated proteins, is a topic of intense debate...
  19. ncbi Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells
    Vered Raz
    Department of Molecular Cell Biology, Leiden University Medical Center, Einsteinweg 20, 2300RC Leiden, The Netherlands
    J Cell Sci 119:4247-56. 2006
    ..We found that caspase-8 induces alteration of the nuclear lamina and a subsequent spatial reorganization of both centromeres, which are shifted towards a peripheral ..
  20. pmc Disruption of nuclear organization during the initial phase of African swine fever virus infection
    Maria Ballester
    Centre de Recerca en Sanitat Animal CReSA, UAB IRTA, Campus de la Universitat Autonoma de Barcelona, Barcelona, 08193, Spain
    J Virol 85:8263-9. 2011
    ....
  21. pmc Generation of induced pluripotent stem cell lines from 3 distinct laminopathies bearing heterogeneous mutations in lamin A/C
    Jenny C Y Ho
    Cardiology Division, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, China
    Aging (Albany NY) 3:380-90. 2011
    ..Lamins are the main constituents of the nuclear lamina, a filamentous meshwork associated with the inner nuclear membrane that provides mechanical stability and ..
  22. ncbi Remodelling of the nuclear lamina and nucleoskeleton is required for skeletal muscle differentiation in vitro
    Ewa Markiewicz
    Integrative Cell Biology Laboratory, School of Biological and Biomedical Sciences, University of Durham, South Road, Durham, DH1 4EB, UK
    J Cell Sci 118:409-20. 2005
    ..In myoblasts transfected with the mutant lamins, this complex was disrupted. These data suggest that remodelling of the nucleoskeleton is necessary for skeletal-muscle differentiation and for correct regulation of Rb pathways...
  23. pmc Laminopathies and the long strange trip from basic cell biology to therapy
    Howard J Worman
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA
    J Clin Invest 119:1825-36. 2009
    The main function of the nuclear lamina, an intermediate filament meshwork lying primarily beneath the inner nuclear membrane, is to provide structural scaffolding for the cell nucleus...
  24. pmc Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism
    Anne Krüger
    Department of Cell Biology, Institute for Biochemistry and Biology, University of Potsdam, 14469 Potsdam Golm, Germany
    Mol Biol Cell 23:360-70. 2012
    Lamins build the nuclear lamina and are required for chromatin organization, gene expression, cell cycle progression, and mechanical stabilization. Despite these universal functions, lamins have so far been found only in metazoans...
  25. ncbi Mutation of SYNE-1, encoding an essential component of the nuclear lamina, is responsible for autosomal recessive arthrogryposis
    Ruben Attali
    Altura Department of Human Genetics, Hebrew University Hospital, Jerusalem, Israel
    Hum Mol Genet 18:3462-9. 2009
    ..These data indicate that mutations of nesprin-1 which interacts with lamin A/C may lead to at least two distinct human disease phenotypes, myopathic or neurological, a feature similar to that found in laminopathies...
  26. ncbi The nuclear lamina and its functions in the nucleus
    Yosef Gruenbaum
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Int Rev Cytol 226:1-62. 2003
    The nuclear lamina is a structure near the inner nuclear membrane and the peripheral chromatin. It is composed of lamins, which are also present in the nuclear interior, and lamin-associated proteins...
  27. pmc Lamin B1 controls oxidative stress responses via Oct-1
    Ashraf N Malhas
    Department of Physics, Sir William Dunn School of Pathology, University of Oxford, Oxford, England, UK
    J Cell Biol 184:45-55. 2009
    ..Sequestration of Oct-1 at the nuclear periphery by lamin B1 may be a mechanism by which the nuclear envelope can regulate gene expression and contribute to the cellular response to stress, development, and aging...
  28. pmc Differential distribution of exosome subunits at the nuclear lamina and in cytoplasmic foci
    Amy C Graham
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106 4960, USA
    Mol Biol Cell 17:1399-409. 2006
    ..Strikingly, dRrp44/dDis3 and dRrp41/dSki6 colocalize with the nuclear lamina and often exhibit a restricted and asymmetric distribution at the nuclear periphery...
  29. pmc The inner nuclear envelope as a transcription factor resting place
    Stijn Heessen
    Department of Tumour Biology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    EMBO Rep 8:914-9. 2007
    ..The mechanisms of inner nuclear membrane association are diverse, and include regulated associations with the nuclear lamina and integral membrane proteins...
  30. pmc The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse
    Jana Link
    Department of Cell and Developmental Biology, Biocenter, University of Wurzburg, Wurzburg, Germany
    PLoS Genet 9:e1003261. 2013
    The nuclear lamina is the structural scaffold of the nuclear envelope and is well known for its central role in nuclear organization and maintaining nuclear stability and shape...
  31. ncbi Transcriptional repression mediated by repositioning of genes to the nuclear lamina
    K L Reddy
    Howard Hughes Medical Institute, The University of Chicago, GCIS W522, 929 East 57th Street, Chicago, Illinois 60637, USA
    Nature 452:243-7. 2008
    ..Although studies on immunoglobulin and other loci have shown a correlation between positioning at the nuclear lamina and gene repression, the functional consequences of this compartmentalization remain untested...
  32. pmc Role of histone deacetylases in gene regulation at nuclear lamina
    Beatrice C Milon
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD, USA
    PLoS ONE 7:e49692. 2012
    ..These findings demonstrate the manifold and central involvement of class I HDACs in regulation of lamina-associated genes, illuminating a mechanism by which these enzymes can orchestrate normal and pathological development...
  33. ncbi Differential expression of nuclear lamin, the major component of nuclear lamina, during neurogenesis in two germinal regions of adult rat brain
    Yasuharu Takamori
    Department of Anatomy and Cell Science, Kansai Medical University, Moriguchi, Osaka, Japan
    Eur J Neurosci 25:1653-62. 2007
    ..Our results suggest that the alteration of nuclear lamina structure is coupled with the progression of neuronal differentiation.
  34. ncbi Genome-nuclear lamina interactions and gene regulation
    Jop Kind
    Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam, The Netherlands
    Curr Opin Cell Biol 22:320-5. 2010
    The nuclear lamina, a filamentous protein network that coats the inner nuclear membrane, has long been thought to interact with specific genomic loci and regulate their expression...
  35. ncbi Lamina-independent lamins in the nuclear interior serve important functions
    T Dechat
    Max F Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Vienna 1030, Austria
    Cold Spring Harb Symp Quant Biol 75:533-43. 2010
    Nuclear lamins were originally described as the main constituents of the nuclear lamina, a filamentous meshwork closely associated with the inner nuclear membrane...
  36. ncbi Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres
    Yasushi Hiraoka
    Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Japan
    Genes Cells 16:1000-11. 2011
    ..Considering that all three INM proteins interact with Sad1, these results suggest that Ima1, Lem2 and Man1 play at least partially redundant roles for nuclear membrane organization...
  37. ncbi The nuclear lamina promotes telomere aggregation and centromere peripheral localization during senescence of human mesenchymal stem cells
    Vered Raz
    Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
    J Cell Sci 121:4018-28. 2008
    ..Intranuclear structures of the nuclear lamina were formed in senescent hMSCs, which are identified by the presence of Hayflick-senescence-associated factors...
  38. ncbi Human laminopathies: nuclei gone genetically awry
    Brian C Capell
    Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, 50 South Drive MSC8004, Bethesda, Maryland 20892 8004, USA
    Nat Rev Genet 7:940-52. 2006
    Few genes have generated as much recent interest as LMNA, LMNB1 and LMNB2, which encode the components of the nuclear lamina. Over 180 mutations in these genes are associated with at least 13 known diseases--the laminopathies...
  39. pmc Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina
    YANIRA GONZALEZ
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX, USA
    Nucleus 3:60-76. 2012
    In animal cells the nuclear lamina, which consists of lamins and lamin-associated proteins, serves several functions: it provides a structural scaffold for the nuclear envelope and tethers proteins and heterochromatin to the nuclear ..
  40. ncbi The nuclear lamina and heterochromatin: a complex relationship
    Erin M Bank
    Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biochem Soc Trans 39:1705-9. 2011
    ..In the present paper, we review current evidence showing that components of the nuclear lamina interact directly with heterochromatin, which implicates the nuclear lamina in a mechanism of specific gene ..
  41. ncbi A-type lamins and Hutchinson-Gilford progeria syndrome: pathogenesis and therapy
    Jose M Gonzalez
    Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas CSIC, Valencia, Spain
    Front Biosci (Schol Ed) 3:1133-46. 2011
    Lamin A and lamin C (A-type lamins, both encoded by the LMNA gene) are major components of the mammalian nuclear lamina, a complex proteinaceous structure that acts as a scaffold for protein complexes that regulate nuclear structure and ..
  42. ncbi The nuclear envelope, a key structure in cellular integrity and gene expression
    V L R M Verstraeten
    Department of Dermatology, University Hospital Maastricht, P Debyelaan 25, PO Box 5800, 6202 AZ Maastricht, The Netherlands
    Curr Med Chem 14:1231-48. 2007
    ..elegans. In this review, we elaborate on the structure and functions of nuclear lamins, the spectrum of syndromes related to mutations in nuclear envelope components and pathogenic concepts unifying these disorders...
  43. doi Hutchinson-Gilford progeria syndrome, aging, and the nuclear lamina
    Bruce Korf
    Department of Genetics at the University of Alabama at Birmingham, Birmingham, USA
    N Engl J Med 358:552-5. 2008
  44. pmc RASCAL is a new human cytomegalovirus-encoded protein that localizes to the nuclear lamina and in cytoplasmic vesicles at late times postinfection
    Matthew S Miller
    Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Virol 84:6483-96. 2010
    ..previously described as sites of nucleocapsid egress, which is mediated by the localized disruption of the nuclear lamina, promoted by the activities of viral and cellular kinases recruited by the lamina-associated proteins UL50 and ..
  45. pmc Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear lamina
    Natalie R Leach
    Program in Molecular and Cellular Biology, University of Iowa, Iowa City, IA 52242, USA
    Virology 406:127-37. 2010
    The nuclear lamina is thought to be a steric barrier to the herpesvirus capsid. Disruption of the lamina accompanied by phosphorylation of lamina proteins is a conserved feature of herpesvirus infection...
  46. pmc Alterations in mitosis and cell cycle progression caused by a mutant lamin A known to accelerate human aging
    Thomas Dechat
    Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 104:4955-60. 2007
    ..These results provide insights into the mechanisms responsible for premature aging and also shed light on the role of lamins in the normal process of human aging...
  47. pmc Epstein-Barr virus BGLF4 kinase induces premature chromosome condensation through activation of condensin and topoisomerase II
    Chung Pei Lee
    Graduate Institute and Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    J Virol 81:5166-80. 2007
    ..involved, we found that transient expression of EBV BGLF4 kinase induces unscheduled chromosome condensation, nuclear lamina disassembly, and stress fiber rearrangements, independently of cellular DNA replication and Cdc2 activity...
  48. ncbi A new model for nuclear lamina organization
    Martin W Goldberg
    School of Biological and Biomedical Sciences, Durham University, Sciences Laboratories, South Road, Durham, UK
    Biochem Soc Trans 36:1339-43. 2008
    ..When lamin A is expressed in oocytes, it forms organized bundles on top of the B lamina. This has led to a new model for lamina organization which is discussed in the present paper...
  49. ncbi Nuclear lamins: key regulators of nuclear structure and activities
    Miron Prokocimer
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Israel
    J Cell Mol Med 13:1059-85. 2009
    The nuclear lamina is a proteinaceous structure located underneath the inner nuclear membrane (INM), where it associates with the peripheral chromatin...
  50. pmc Novel mode of phosphorylation-triggered reorganization of the nuclear lamina during nuclear egress of human cytomegalovirus
    Jens Milbradt
    Institute for Clinical and Molecular Virology, University of Erlangen Nuremberg, D 91054 Erlangen, Germany
    J Biol Chem 285:13979-89. 2010
    ..viral and cellular proteins, in particular protein kinases with the capacity to induce destabilization of the nuclear lamina. Viral protein kinase pUL97 and cellular protein kinase C (PKC) play important roles by phosphorylating ..
  51. pmc Herpes simplex virus 2 UL13 protein kinase disrupts nuclear lamins
    Gina L Cano-Monreal
    Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1100 South Grand Blvd, St Louis, MO 63104, USA
    Virology 392:137-47. 2009
    ....
  52. ncbi The nuclear lamina comes of age
    Yosef Gruenbaum
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem, 91904 Israel
    Nat Rev Mol Cell Biol 6:21-31. 2005
    ....
  53. ncbi Caenorhabditis elegans as a model system for studying the nuclear lamina and laminopathic diseases
    Erin M Bank
    Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem 91904, Israel
    Nucleus 2:350-7. 2011
    The nuclear lamina is a protein-rich network located directly underneath the inner nuclear membrane of metazoan nuclei...
  54. pmc Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis
    Donald D Anderson
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 287:7051-62. 2012
    ..In this study, we demonstrate that this pathway forms a multienzyme complex that is associated with the nuclear lamina. SHMT1 or SHMT2α is required for co-localization of dihydrofolate reductase, SHMT, and thymidylate synthase ..
  55. ncbi Phosphorylation of the p34(cdc2) target site on goldfish germinal vesicle lamin B3 before oocyte maturation
    Akihiko Yamaguchi
    Laboratory of Marine Biology, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Hakozaki 6 10 1, Higashi ku, Fukuoka 812 8581, Japan
    Eur J Cell Biol 85:501-17. 2006
    ..The mechanisms by which lamin B3 is transported into GVs and is assembled to form the nuclear lamina are not well understood...
  56. ncbi Lamins, laminopathies and disease mechanisms: possible role for proteasomal degradation of key regulatory proteins
    Veena K Parnaik
    Centre for Cellular and Molecular Biology CSIR, Hyderabad 500 007, India
    J Biosci 36:471-9. 2011
    ..New findings on the role of lamins in cellular signalling pathways, as well as in ubiquitin-mediated proteasomal degradation, have given important insights into possible mechanisms of pathogenesis...
  57. pmc Age-related changes of nuclear architecture in Caenorhabditis elegans
    Erin Haithcock
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Proc Natl Acad Sci U S A 102:16690-5. 2005
    ..Because the nucleus is vital for many cellular functions, our studies raise the possibility that the nucleus is a prominent focal point for regulating aging...
  58. pmc A laminopathic mutation disrupting lamin filament assembly causes disease-like phenotypes in Caenorhabditis elegans
    Erin M Bank
    Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Mol Biol Cell 22:2716-28. 2011
    ..results show that changes in lamin filament structure can translate into disease-like phenotypes via altering the localization of nuclear lamina proteins, and suggest a model for how the ΔK32 lamin mutation may cause EDMD in humans.
  59. pmc Human cytomegalovirus UL50 and UL53 recruit viral protein kinase UL97, not protein kinase C, for disruption of nuclear lamina and nuclear egress in infected cells
    Mayuri Sharma
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 88:249-62. 2014
    ..the nuclear envelope into the cytoplasm in a process called nuclear egress that includes disruption of the nuclear lamina. In several herpesviruses, a key player in nuclear egress is a complex of two proteins, whose homologs in ..
  60. ncbi DelK32-lamin A/C has abnormal location and induces incomplete tissue maturation and severe metabolic defects leading to premature death
    Anne T Bertrand
    INSERM, UMRS_974, Paris F 75013, France
    Hum Mol Genet 21:1037-48. 2012
    ..Taken together, our results suggest that lamin A/C relocation at the nuclear lamina seems important for tissue maturation potentially by releasing its inhibitory function on transcriptional ..
  61. pmc Lamin A/C mutation analysis in a cohort of 324 unrelated patients with idiopathic or familial dilated cardiomyopathy
    Sharie B Parks
    Division of Cardiovascular Medicine, Oregon Health and Science University, Portland, OR, USA
    Am Heart J 156:161-9. 2008
    ..We sought to examine the frequency of mutations in LMNA, the gene encoding lamin A/C, in patients with idiopathic (IDC) or familial dilated cardiomyopathy (FDC)...
  62. pmc Apoptosis leads to a degradation of vital components of active nuclear transport and a dissociation of the nuclear lamina
    A Kramer
    Institute of Physiology II, Westfalische Wilhelms Universitat Munster, Robert Koch Strasse 27b, 48149 Munster, Germany
    Proc Natl Acad Sci U S A 105:11236-41. 2008
    ..contributes significantly to the overall structure and mechanical stability of the cell nucleus through the nuclear lamina, which underlies the entire nucleoplasmic face of the NE and thereby interconnects the NPCs, the NE, and the ..
  63. pmc The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling
    Haiyang Chen
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Cell Stem Cell 13:73-86. 2013
    ..Thus, we have uncovered a role for the nuclear lamina in the integration of EGF signaling to regulate stem cell niche function.
  64. ncbi The nuclear envelope lamina network has elasticity and a compressibility limit suggestive of a molecular shock absorber
    Kris Noel Dahl
    Department of Chemical and Biomolecular Engineering, 220 South 33rd Street, University of Pennsylvania, Philadelphia, PA 19104 6393, USA
    J Cell Sci 117:4779-86. 2004
    ..Our results suggest that the nuclear lamina forms a compressed network shell of interconnected rods that is extensible but limited in compressibility from ..
  65. ncbi Emerin and the nuclear lamina in muscle and cardiac disease
    James M Holaska
    Department of Medicine, Section of Cardiology, University of Chicago, 5841 S Maryland Ave, MC 6088, Chicago, IL 60637, USA
    Circ Res 103:16-23. 2008
    ....
  66. pmc The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C
    Magdalena Zaremba-Czogalla
    Laboratory of Nuclear Proteins, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
    PLoS ONE 7:e32649. 2012
    ..modifications is practically limited to the function of cdc 2 (cdk1) kinase sites in depolymerization of the nuclear lamina during mitosis...
  67. ncbi Natural history of dilated cardiomyopathy due to lamin A/C gene mutations
    Matthew R G Taylor
    University of Colorado Cardiovascular Institute, Denver, Colorado 80010, USA
    J Am Coll Cardiol 41:771-80. 2003
    ..We examined the prevalence, genotype-phenotype correlation, and natural history of lamin A/C gene (LMNA) mutations in subjects with dilated cardiomyopathy (DCM)...
  68. ncbi Three-dimensional organization of chromatids by nuclear envelope-associated structures
    Gunter Blobel
    Laboratory of Cell Biology, The Rockefeller University Howard Hughes Medical Institute, New York, New York 10065, USA
    Cold Spring Harb Symp Quant Biol 75:545-54. 2010
    ..NE-associated structures, such as nuclear pore complexes (NPCs), the nuclear lamina, and nuclear envelope junctions (NEJs), have evolved to organize, among other things, chromatids within the ..
  69. pmc LMNA cardiomyopathy: cell biology and genetics meet clinical medicine
    Jonathan T Lu
    Department of Medicine, Columbia University, New York, NY 10032, USA
    Dis Model Mech 4:562-8. 2011
    ....
  70. ncbi Lamins: building blocks or regulators of gene expression?
    Christopher J Hutchison
    Department of Biological and Biomedical Sciences, University of Durham, South Road, Durham DH1 3LE, UK
    Nat Rev Mol Cell Biol 3:848-58. 2002
    ..These influences require the lamins to have dynamic properties and dual identities--as building blocks and transcriptional regulators. Which one of these identities underlies a myriad of genetic diseases is a topic of intense debate...
  71. pmc Functional coupling between the extracellular matrix and nuclear lamina by Wnt signaling in progeria
    Lidia Hernandez
    Cancer and Developmental Biology Laboratory, NCI, Frederick, MD 21702, USA
    Dev Cell 19:413-25. 2010
    ..These results establish a functional link between the nuclear envelope/lamina and the cell surface/ECM and may provide insights into the role of Wnt signaling and the ECM in aging...
  72. ncbi Cytomegaloviral proteins that associate with the nuclear lamina: components of a postulated nuclear egress complex
    Jens Milbradt
    Institute for Clinical and Molecular Virology, University of Erlangen Nuremberg, 91054 Erlangen, Germany
    J Gen Virol 90:579-90. 2009
    ..capsids traversing the nuclear envelope is dependent on a locally restricted destabilization of the rigid nuclear lamina. It has been suggested that the multi-component nuclear egress complex (NEC) that is formed is comprised of ..
  73. pmc Mapping of protein- and chromatin-interactions at the nuclear lamina
    Nard Kubben
    Center for Heart Failure Research, Maastricht, The Netherlands
    Nucleus 1:460-71. 2010
    ..Here we summarize and critically discuss recent technology to analyze lamina-protein and-chromatin interactions...
  74. pmc Reorganization of the nuclear lamina and cytoskeleton in adipogenesis
    Valerie L R M Verstraeten
    Department of Dermatology, Maastricht University Medical Centre, P Debyelaan 25, P O Box 5800, 6202 AZ, Maastricht, The Netherlands
    Histochem Cell Biol 135:251-61. 2011
    ..Although molecular pathways governing adipogenesis are well delineated, the structure of the nuclear lamina and nuclear-cytoskeleton junction in this process are not...
  75. ncbi Blurring the boundary: the nuclear envelope extends its reach
    Colin L Stewart
    Institute of Medical Biology, 61 Biopolis Drive, Proteos, Singapore 138668, Singapore
    Science 318:1408-12. 2007
    ..It is becoming apparent that the nuclear envelope defines not only nuclear organization but also that of the cytoskeleton and, in this way, integrates both nuclear and cytoplasmic architecture...
  76. ncbi Lamin A and ZMPSTE24 (FACE-1) defects cause nuclear disorganization and identify restrictive dermopathy as a lethal neonatal laminopathy
    Claire L Navarro
    INSERM U491, Génétique Médicale et Développement, Faculte de Medecine de Marseille, Hopital d Enfants de la Timone, Marseille, France
    Hum Mol Genet 13:2493-503. 2004
    ..Lamins are major constituents of the nuclear lamina, a filamentous meshwork underlying the inner nuclear envelope...
  77. ncbi Remodelling of the nuclear lamina during human cytomegalovirus infection: role of the viral proteins pUL50 and pUL53
    Daria Camozzi
    Department of Clinical and Experimental Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
    J Gen Virol 89:731-40. 2008
    ..In the family Herpesviridae, this process involves alteration of nuclear lamina components by two highly conserved proteins, whose homologues in HCMV are named pUL50 and pUL53...
  78. pmc Viral mimicry of Cdc2/cyclin-dependent kinase 1 mediates disruption of nuclear lamina during human cytomegalovirus nuclear egress
    Sofia Hamirally
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 5:e1000275. 2009
    The nuclear lamina is a major obstacle encountered by herpesvirus nucleocapsids in their passage from the nucleus to the cytoplasm (nuclear egress)...
  79. pmc Dynamics and molecular interactions of linker of nucleoskeleton and cytoskeleton (LINC) complex proteins
    Cecilia Ostlund
    Department of Medicine, Columbia University, New York, NY 10032, USA
    J Cell Sci 122:4099-108. 2009
    ..Our data support a model in which A-type lamins and Sun2 anchor nesprin-2 in the outer nuclear membrane, whereas emerin, Sun1 and actin are dispensable for this anchoring...
  80. ncbi Nuclear lamins: laminopathies and their role in premature ageing
    J L V Broers
    Department of Molecular Cell Biology, University of Maastricht, Research Institutes CARIM, GROW, and EURON, The Netherlands
    Physiol Rev 86:967-1008. 2006
    ..It is concluded that lamins seem to be key players in, among others, controlling the process of cellular ageing, since disturbance in lamin protein structure gives rise to several forms of premature ageing...
  81. pmc Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress
    Susan L Bjerke
    Department of Microbiology, The University of Iowa, 3115 Medical Laboratories, Iowa City, IA 52242, USA
    Virology 347:261-76. 2006
    ..The data presented in this paper argue for crucial roles for both UL34 and US3 in regulating the state of the nuclear lamina during viral infection.
  82. pmc Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3
    Natalie Leach
    Department of Microbiology, The University of Iowa, 3 432 Bowen Science Building, Iowa City, IA 52242, USA
    J Virol 81:10792-803. 2007
    Cells infected with wild-type herpes simplex virus type 1 (HSV-1) show disruption of the organization of the nuclear lamina that underlies the nuclear envelope...
  83. ncbi Invertebrate lamins
    Shai Melcer
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Exp Cell Res 313:2157-66. 2007
    Lamins are the main component of the nuclear lamina and considered to be the ancestors of all intermediate filament proteins...
  84. pmc Epstein-Barr virus BGLF4 kinase induces disassembly of the nuclear lamina to facilitate virion production
    Chung Pei Lee
    Graduate Institute and Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan
    J Virol 82:11913-26. 2008
    ..we found that Ser-22, Ser-390, and Ser-392 of lamin A are important for the BGLF4-induced disassembly of the nuclear lamina and the EBV reactivation-mediated redistribution of nuclear lamin...
  85. pmc A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence
    R Ileng Kumaran
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    J Cell Biol 180:51-65. 2008
    The peripheral nuclear lamina, which is largely but not entirely associated with inactive chromatin, is considered to be an important determinant of nuclear structure and gene expression...
  86. pmc Nuclear envelope dispersion triggered by deregulated Cdk5 precedes neuronal death
    Kuei Hua Chang
    Department of Chemistry and Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA
    Mol Biol Cell 22:1452-62. 2011
    ..In this study, we show that nuclear lamina dispersion is an early and irreversible trigger for cell death initiated by deregulated Cdk5, rather than a ..
  87. pmc Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex
    Lisa A Hawryluk-Gara
    Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Mol Biol Cell 16:2382-94. 2005
    ..On basis of these data, we propose a model in which Nup53 is positioned near the pore membrane and the lamina where it anchors an NPC subcomplex containing Nup93, Nup155, and Nup205...
  88. ncbi Role of nuclear lamins in nuclear organization, cellular signaling, and inherited diseases
    Veena K Parnaik
    Center for Cellular and Molecular Biology, Hyderabad, India
    Int Rev Cell Mol Biol 266:157-206. 2008
    ..The observed interactions of lamins with inner nuclear membrane proteins, chromatin, and various regulatory factors have given important insights into the role of lamins in cellular processes and tissue-specific signaling pathways...
  89. ncbi Intranuclear membrane structure formations by CaaX-containing nuclear proteins
    Thorsten Ralle
    Department of Cell Biology, University of Bremen, PO Box 33 04 40, 28334 Bremen, Germany
    J Cell Sci 117:6095-104. 2004
    The nuclear lamina is a protein meshwork lining the nucleoplasmic face of the nuclear envelope. Association of lamins with the inner nuclear membrane is mediated by specific modifications in the CaaX motif at their C-termini...
  90. pmc Conformational changes in the nuclear lamina induced by herpes simplex virus type 1 require genes U(L)31 and U(L)34
    Ashley E Reynolds
    Dept of Microbiology and Immunology, VMC C5 131, Cornell University, Ithaca, NY 14853, USA
    J Virol 78:5564-75. 2004
    ..solubility of lamins A and C (lamin A/C) was not markedly increased, HSV induced conformational changes in the nuclear lamina of infected cells, as viewed after staining with three different lamin A/C-specific antibodies...
  91. ncbi Role of nuclear lamina-cytoskeleton interactions in the maintenance of cellular strength
    F Houben
    Department of Molecular Cell Biology, Cardiovascular Research Institute Maastricht CARIM and Research Institute for Growth and Development GROW, University of Maastricht, Maastricht, The Netherlands
    Biochim Biophys Acta 1773:675-86. 2007
    ..Several new interconnecting proteins, such as the nesprins that link the nuclear lamina to the cytoskeleton, have been identified...
  92. ncbi LEM-Domain proteins: new insights into lamin-interacting proteins
    Nicole Wagner
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, S 10691 Stockholm, Sweden
    Int Rev Cytol 261:1-46. 2007
    ..All these findings as well as previously proposed ideas and models have been summarized to broaden our view of this exciting protein family...
  93. ncbi Characterization of the Drosophila melanogaster genome at the nuclear lamina
    Helen Pickersgill
    Department of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    Nat Genet 38:1005-14. 2006
    The nuclear lamina binds chromatin in vitro and is thought to function in its organization, but genes that interact with it are unknown...
  94. ncbi Cellular p32 recruits cytomegalovirus kinase pUL97 to redistribute the nuclear lamina
    Manfred Marschall
    Institute for Clinical and Molecular Virology, University of Erlangen Nurnberg, Erlangen 91054, Germany
    J Biol Chem 280:33357-67. 2005
    ..at the level of nucleocytoplasmic transport of viral capsids, a process that requires the disassembly of the nuclear lamina. Deletion of the protein kinase gene UL97 from the viral genome showed that the activity of pUL97 plays an ..
  95. ncbi Electron microscopy of lamin and the nuclear lamina in Caenorhabditis elegans
    Merav Cohen
    Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom
    Methods Cell Biol 88:411-29. 2008
    The nuclear lamina is found between the inner nuclear membrane and the peripheral chromatin...
  96. pmc Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy
    Lothar Schermelleh
    Center for Integrated Protein Science, Department of Biology, Ludwig Maximilians University Munich, 82152 Planegg Martinsried, Germany
    Science 320:1332-6. 2008
    ..By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional ..
  97. pmc Defects in lamin B1 expression or processing affect interphase chromosome position and gene expression
    Ashraf Malhas
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, England, UK
    J Cell Biol 176:593-603. 2007
    ..We also demonstrate that the position of mouse chromosome 18 but not 19 is dependent on such a stable nuclear lamina. The results implicate processed lamin B1 in the control of gene expression as well as chromosome position.
  98. ncbi The nuclear lamina and its proposed roles in tumorigenesis: projection on the hematologic malignancies and future targeted therapy
    Miron Prokocimer
    Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Struct Biol 155:351-60. 2006
    The nuclear lamina, a network of lamin filaments and lamin-associated proteins, is located between the inner nuclear membrane and the peripheral chromatin...
  99. pmc Herpes simplex virus type 1 infection induces activation and recruitment of protein kinase C to the nuclear membrane and increased phosphorylation of lamin B
    Richard Park
    Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853, USA
    J Virol 80:494-504. 2006
    ..The data suggest a model in which kinases that normally disassemble the nuclear lamina during apoptosis are recruited to the nuclear membrane through functions requiring UL31 and UL34...
  100. pmc Characterization and intracellular localization of the Epstein-Barr virus protein BFLF2: interactions with BFRF1 and with the nuclear lamina
    Roberta Gonnella
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Medicina Sperimentale e Patologia, Universita di Roma La Sapienza, Rome, Italy
    J Virol 79:3713-27. 2005
    ..biochemical and morphological analysis showed association of the BFRF1-BFLF2 complex with a component of the nuclear lamina, lamin B. Taken together, these results and those of the accompanying paper (Farina et al., J. Virol...
  101. pmc A mechanism of AP-1 suppression through interaction of c-Fos with lamin A/C
    Carmen Ivorra
    Laboratory of Vascular Biology, Department of Molecular and Cellular Pathology and Therapy, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Valencia 46010, Spain
    Genes Dev 20:307-20. 2006
    ..We propose lamin A/C-mediated c-Fos sequestration at the NE as a novel mechanism of transcriptional and cell cycle control...

Research Grants62

  1. Mechanisms of Stem Cell Self-Renewal and Aging
    PAUL CHRISTIAN SCHILLER; Fiscal Year: 2013
    ..Accelerated senescence syndromes caused by accumulation of unprocessed farnesylated nuclear lamina protein lamin-A (key in nuclear architecture), due to alterations in the lamin A gene (LMNA) mRNA splicing, ..
  2. The Role of the Nuclear Lamina in Chromatin Organization and Gene Expression in N
    Marcos Estecio; Fiscal Year: 2012
    ..coats the intranuclear portion of the nuclear envelope and, along with other structural proteins, forms the nuclear lamina. This structure has activity in key cellular functions, such as DNA replication, cell division and apoptosis...
  3. INTERACTIONS BETWEEN INTERMEDIATE FILAMENTS AND NUCLEUS
    Robert D Goldman; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Lamins are intermediate filament proteins that are found in the nuclear lamina and in the nucleoplasm...
  4. GENETICS OF HERPESVIRUS TRANSFORMATION
    David M Knipe; Fiscal Year: 2013
    ..Define the mechanisms of chromatin regulation on viral DNA through targeting of the viral genome to the nuclear lamina in studies to determine the viral and host gene products needed for peripheral targeting of viral genome ..
  5. Studying the role of the nuclear lamina in age dependent demyelination
    Quasar S Padiath; Fiscal Year: 2013
    ..In eukaryotic cells, lamin B1 is a major constituent of the nuclear lamina, a fibrous meshwork adjacent to the inner nuclear membrane...
  6. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2012
    ..eukaryotes, once chromosomes have undergone condensation, the nuclear membranes, nuclear pore complexes, and nuclear lamina are disassembled in concert...
  7. The role of Lamin B1 in myelin maintenance and demyelination
    Ying Hui Fu; Fiscal Year: 2010
    ..Patients carry an extra copy of the gene for the nuclear lamina protein, Lamin B1, resulting in increased gene dosage in ADLD brain tissue...
  8. Nuclear lamina in regulation of signaling in muscle
    LARRY R GERACE; Fiscal Year: 2013
    ..applicant): A broad spectrum of human diseases ("laminopathies") is caused by mutations in components of the nuclear lamina, a protein meshwork that lines the nuclear envelope...
  9. Mechanisms of how nuclear envelope bridges link nuclei to the cytoskeleton.
    Daniel A Starr; Fiscal Year: 2013
    ..We hypothesize that forces generated in the cytoplasm are directly linked to the nuclear lamina by KASH-SUN bridges. Our approach is innovative because it takes advantage of a C...
  10. Structure-Function of the Nuclear Envelope Bridge and its Role in Laminopathies
    Thomas U Schwartz; Fiscal Year: 2012
    ..B-type lamins, a meshwork of proteins that lines the nucleoplasmic face of the nuclear envelope and forms the nuclear lamina. Mutations scattered along LMNA, which encodes A-type lamins, as well as mutations within other nuclear ..
  11. The Nucleocytoskeleton in Progeria and Aging
    Howard J Worman; Fiscal Year: 2013
    ..site resulting in the abnormal accumulation of farnesylated prelamin A variant called progerin in the nuclear lamina. Mutations in the ZMPSTE24 gene encoding the protease that processes prelamin A result in a similar ..
  12. Human Cytomegalovirus Nuclear Egress: Molecular Mechanisms and Drug Targeting
    James M Hogle; Fiscal Year: 2013
    ..particular, whether the only role of UL97 in nuclear egress is phosphorylation of lamin A/C to disrupt the nuclear lamina. A principal approach will be to analyze a recombinant HCMV expressing a dominant negative mutant of lamin A/C ..
  13. Control of TCR V Beta Rearrangement &Allelic Exclusion
    Michael S Krangel; Fiscal Year: 2013
    ..Specific Aim II will test the hypothesis that Tcrb association with the nuclear lamina suppresses Vβ-to-DJβrecombination in a manner that promotes asynchronous allelic ..
  14. CaaX Protein Processing and Human Disease
    Stephen G Young; Fiscal Year: 2013
    ..Aim 2 is to define the importance of the posttranslational modifications of prelamin A in the assembly of the nuclear lamina, and to define the importance of protein farnesylation in the pathogenesis of progeroid syndromes...
  15. The role of nucleo-cytoskeletal link proteins in skeletal muscle
    Ju Chen; Fiscal Year: 2013
    ..The INM, the outer nuclear membrane (ONM), and the nuclear lamina comprise the nuclear envelope, which is linked to the cytokeleton by members of both the SUN and Nesprin ..
  16. The Frizzled Nuclear Import Pathway in Synapse Development
    VIVIAN G BUDNIK; Fiscal Year: 2013
    ..Protein Kinase C, Par3/Bazooka and Dap160/Intersectin) as important determinants of local remodeling of the nuclear lamina during nuclear megaRNP egress...
  17. Nucleocapsid envelopment Herpes simplex virus-1
    Joel D Baines; Fiscal Year: 2013
    ..separate aspects of the envelopment reaction that comprise the rest of the application: 1) Disruption of the nuclear lamina to provide nucleocapsids access to budding sites in the INM and 2) Construction of budding sites at the INM...
  18. Human Cytomegalovirus Nuclear Egress: Molecular Mechanisms and Drug Targeting
    Donald Coen; Fiscal Year: 2009
    ..Why the NEC is not sufficient to disrupt nuclear lamina in infected cells in the absence of UL97 will be studied...
  19. Nuclear structure in the development of lupus autoimmunity
    Michael F Denny; Fiscal Year: 2013
    ..The LBR stabilizes nuclear structure by binding both chromatin and the nuclear lamina. LBR located within the SLEB1 lupus susceptibility interval on 1q42 in humans, and in the syntenic regions ..
  20. The role of LEM domain proteins in nuclear function
    Pamela K Geyer; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Abstract: The nuclear lamina is a protein meshwork underneath the nuclear envelope (NE) that contributes to chromosome organization and gene regulation...
  21. The nucleus-cytoskeleton connection in health and disease
    Denis Wirtz; Fiscal Year: 2011
    ..is conferred by A- and B-type lamins, a meshwork of proteins that underlies the nuclear envelope and forms the nuclear lamina. Mutations scattered along Lmna, which encodes A-type lamins, are associated to a broad range of human ..
  22. FIRCA: Probing the Nucleus- Cytoskeleton Connection Using Magnetic Tweezers
    Denis Wirtz; Fiscal Year: 2013
    ..R01GM08420401, 09/30/08 - 08/31/12." The nuclear envelope has an internal shell called nuclear lamina, a thin meshwork of intermediate filaments composed of A- and B-type lamins...
  23. Regulation of nuclear transport in disease
    BRYCE PASCHAL; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Mutations in structural components of the nuclear lamina cause a spectrum of human diseases known as laminopathies...
  24. Super-resolution microscopy of nuclear lamin and spindle envelope/matrix function
    Yixian Zheng; Fiscal Year: 2013
    ..As animal cells enter mitosis, parts of the interphase membrane network, including the nuclear lamina, and nuclear proteins re-organize into an inter-connected network that both surrounds and permeates the ..
  25. Matrix-induced Myogenesis & Pharmaco-Screens of MSCs
    Dennis Discher; Fiscal Year: 2009
    ..The nuclear lamina interacts with chromatin and through linker proteins that span the envelope the lamina also interacts with the ..
  26. PATHOBIOLOGY OF THE NUCLEAR ENVELOPE IN CANCER
    Howard Worman; Fiscal Year: 2000
    ..in the diagnosis of leukemias and small cell lung carcinomas which do not express two proteins of the nuclear lamina called lamin A and lamin C...
  27. The meiotic role of dtopors in male Drosophila
    JOHN TOMKIEL; Fiscal Year: 2007
    ..lamin and has been hypothesized to play an important functional role in tethering insulator complexes to the nuclear lamina. The Dtopors protein is also essential for proper chromosome segregation in the male germ line, where it is ..
  28. Role of the Human Cytomegalovirus UL97 Proein Kinase in Viral Replication
    JEREMY KAMIL; Fiscal Year: 2009
    ..Interestingly, lamin A/C, a major component of the nuclear lamina, specifically undergoes UL97-dependent changes in its phosphorylation pattern during HCMV infection...
  29. Posttranslational Processing by Zmpste24 and Laminopathy
    Jennifer Ng; Fiscal Year: 2005
    The proteins of the nuclear lamina have generated enormous interest because missense mutations in LMNA (the gene for prelamin A, which encodes both lamin A and lamin C) cause a host of diseases, including Emery-Dreifuss muscular dystrophy,..
  30. HEAT AND RADIATION INDUCED CHANGES IN THE NUCLEAR MATRIX
    JOSEPH DYNLACHT; Fiscal Year: 2000
    ..heat-induced changes in nuclear lamin and interior matrix proteins, we will quantitate heat-induced changes in nuclear lamina nd interior matrix proteins, we will quantitate heat-induced changes in nuclear lamin and interior matrix ..
  31. UL34 and herpes simplex virus envelopment
    Richard J Roller; Fiscal Year: 2010
    ..3. We will fully characterize changes in the host cell nuclear lamina associated with infection and determine the mechanism by which UL34 and other viral proteins accomplish those ..
  32. TRANSMISSION ELECTRON MICROSCOPE
    William Brown; Fiscal Year: 2000
    ..Chen), the role of the nuclear lamina protein Ya that is essential for the transition from meiosis to mitosis in the fertilized Drosophila egg (Dr...
  33. Lamin A in Adipocyte Differentation and Survival
    Howard Worman; Fiscal Year: 2004
    ..Confirmation of this hypothesis will have implications for the identification of new cellular targets to treat human obesity and diabetes mellitus. ..
  34. Lamin A Mutation and Hutchinson-Gilford Progeria
    Howard Worman; Fiscal Year: 2008
    ..This project will establish how mutations in nuclear lamins A and C cause HGPS, and if inhibition of protein farnesylation is a potential therapeutic intervention. ..
  35. DUX4 and Facioscapulohumeral Muscular Dystrophy
    Howard Worman; Fiscal Year: 2004
    ..This work could lead to the development of new diagnostic methods as well as the identification of potential protein targets for the treatment of FSHD. ..
  36. THE NHLBI BAY AREA FUNCTIONAL GENOMICS COMSORTIUM
    Stephen Young; Fiscal Year: 2003
    ..The Consortium's resources will be distributed freely to any interested investigator and should provide a catalyst for many different NHLBI-funded research programs. ..
  37. METABOLISM OF SPONTANEOUSLY DAMAGED PROTEINS IN AGING
    Stephen Young; Fiscal Year: 2002
    ..Finally, we propose to ask whether the expression of the repair methyltransferase in the extracellular compartment would limit the accumulation of spontaneous damage to extracellular proteins. ..
  38. MECHANISMS OF NUCLEAR IMPORT
    Larry Gerace; Fiscal Year: 2006
    ..Thus, understanding basic nuclear transport mechanisms can provide a framework for developing new therapeutic approaches relevant to viral pathogenesis, cancer and immunity and other processes. ..
  39. Import of Adenovirus DNA into the nucleus
    Larry Gerace; Fiscal Year: 2007
    ..Finally, it could provide the basis for developing efficient means for nonviral gene transfer, which would be useful for gene therapy and functional studies of cells. ..
  40. CAAX Protein Processing and Cancer Therapy
    Stephen Young; Fiscal Year: 2007
    ..These studies should define the therapeutic potential of blocking the three different steps of CAAX protein processing. ..
  41. NHLBI Bay Area Functional Genomics Consortium
    Stephen Young; Fiscal Year: 2007
    ..A fourth objective is to generate a limited number of genetically modified mice, for the purpose of defining the relevance of specific genes to cardiopulmonary development and disease (Components 4-6). ..
  42. Postisoprenylation Processing and the Nuclear Lamina
    Stephen Young; Fiscal Year: 2007
    The proteins of the nuclear lamina have generated enormous interest because of recent studies showing that mutations in the gene for lamin A/C (LMNA) develop a host of different diseases, including cardiomyopathy, muscular dystrophy, and ..
  43. Screen for Small Molecule Inhibitors of Nuclear Protein Import
    Larry Gerace; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  44. Hutchinson Gilford Progeria Syndrome and Atherosclerosis
    Stephen Young; Fiscal Year: 2009
    ..The PI's laboratory has extensive experience in analyzing atherogenesis in mice and is thrilled with the prospect of exploring mechanisms of atherosclerosis in HGPS. ..
  45. ASCB Summer Meeting: Nuclear Architecture and Disease
    Katherine Wilson; Fiscal Year: 2005
    ..The meeting will foster intense informal discussion in search of common principals and will stimulate interest and progress in this emerging 'post-genomic' field. ..
  46. Effect of Estrogen on Radiation-induced Cataractogenesis
    JOSEPH DYNLACHT; Fiscal Year: 2005
    ..abstract_text> ..
  47. INTERMEDIATE FILAMENT CELL SURFACE INTERACTIONS
    Robert Goldman; Fiscal Year: 2008
    ..The latter include the formation of Mallory bodies in alcoholic liver disease and Lewy bodies in Parkinson's disease. ..
  48. Functional Analysis of LEM-domain Nuclear Proteins
    Katherine Wilson; Fiscal Year: 2005
    ..This work will be the first genetic analysis of the nuclear lamina. Our work will yield basic knowledge about the functions of LEM proteins, and their interactions with lamins ..
  49. Emerin functions in transcriptional regulation
    James Holaska; Fiscal Year: 2004
    ..One of these proteins, emerin, directly binds the nuclear lamina and a chromatin associated protein named Barrier-to-Autointegration (BAF)...
  50. MECHANISMS OF MUCOSAL IMMUNITY TO HERPES SIMPLEX VIRUS
    LYNDA MORRISON; Fiscal Year: 2002
    ..This information will facilitate developent of immunization strategies against HSV and possibly other pathogens of the genital tract. ..
  51. DEVELOPMENT OF A LIVE ATTENUATED HSV-2 VACCINE
    Mark Prichard; Fiscal Year: 2002
    ..In addition further in vitro characterization will enable high quality clinical trial material to be produced for IND enabling primate studies and early Phase I human trials. PROPOSED COMMERCIAL APPLICATION: Not Available ..
  52. Substrate recognition motif of the HSV-2 UL 13 kinase
    LYNDA MORRISON; Fiscal Year: 2006
    ..Our long-range goal is to use the novel viral targets identified through application of the UL13 recognition motif to elucidate the mechanisms by which UL13 regulates viral replication and pathology. [unreadable] [unreadable]..
  53. INTERMEDIATE FILAMENT CELL SURFACE INTERACTIONS
    Robert D Goldman; Fiscal Year: 2010
    ....
  54. Familial Dilated Cardiomyopathy:Genetic Characterization
    Matthew Taylor; Fiscal Year: 2006
    ..In addition, a diverse collection of consultants has been assembled to provide ongoing expertise that is sensitive to the needs of both the research and career development plans of the candidate. ..
  55. Vaccine to Prevent HSV-induced keratitis
    LYNDA MORRISON; Fiscal Year: 2006
    ..Aim 3: We will determine immunogenicity, and prophylactic and therapeutic efficacy of ICP8-/vhs- /B7+ HSV BAC as a DNA vaccine versus ICP8-/vhs- /B7+ virus vaccine. [unreadable] [unreadable]..
  56. LAMIN A PROGERIA MUTATIONS AND NUCLEAR FUNCTION
    Robert D Goldman; Fiscal Year: 2010
    ..HGPS is caused by mutations in human lamin A (hLA), a protein component of the nuclear lamina. The long-term objective of the proposed research is to determine the molecular basis by which mutations in ..
  57. Lipoprotein Metabolism 2008 Gordon Research Conference
    Robert Hegele; Fiscal Year: 2008
    ..End of Abstract) [unreadable] [unreadable] [unreadable]..
  58. The Role of the Mre11 Complex in Heat-radiosensitization
    JOSEPH DYNLACHT; Fiscal Year: 2007
    ..We will also determine the role of HR in heat-radiosensitization using silencing RNA (siRNA) targeting technology or conditional mutants deficient in specific NHEJ or HR proteins. [unreadable] [unreadable]..
  59. Functional analysis of HSV-2 tegument proteins in mice
    LYNDA MORRISON; Fiscal Year: 2008
    ..These investigations will improve our understanding of the roles of the UL13 and vhs enzymes in HSV-2 pathogenesis. ..
  60. RED CELL CYTOSKELETAL PROTEIN 4.1 DURING CELL DIVISION
    SHARON KRAUSS; Fiscal Year: 2008
    ..1 gene defects. However, our investigations will also contribute a broad perspective on molecular disease loci involving defective or deficient cytoskeletal proteins. ..
  61. Molecular genetics of egg activation and pronuclear function in Drosophila
    MARIANA FEDERICA WOLFNER; Fiscal Year: 2010
    ..YA associate with chromatin and with the nuclear lamina, to permit mitosis by coordinating the cell cycles of the pronuclei...