gap junctions

Summary

Summary: Connections between cells which allow passage of small molecules and electric current. Gap junctions were first described anatomically as regions of close apposition between cells with a narrow (1-2 nm) gap between cell membranes. The variety in the properties of gap junctions is reflected in the number of CONNEXINS, the family of proteins which form the junctions.

Top Publications

  1. ncbi Plasma membrane channels formed by connexins: their regulation and functions
    Juan C Saez
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    Physiol Rev 83:1359-400. 2003
  2. ncbi Tumor necrosis factor-alpha induces neurotoxicity via glutamate release from hemichannels of activated microglia in an autocrine manner
    Hideyuki Takeuchi
    Department of Neuroimmunology, Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Japan
    J Biol Chem 281:21362-8. 2006
  3. ncbi Pannexin membrane channels are mechanosensitive conduits for ATP
    Li Bao
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 572:65-8. 2004
  4. ncbi An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion
    Taizo Kawano
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON M5G1X5, Canada
    Neuron 72:572-86. 2011
  5. pmc Gap junctions
    Daniel A Goodenough
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 1:a002576. 2009
  6. ncbi Gap junctions and the connexin protein family
    Goran Sohl
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, 53117 Bonn, Germany
    Cardiovasc Res 62:228-32. 2004
  7. ncbi Ischemia opens neuronal gap junction hemichannels
    Roger J Thompson
    Department of Psychiatry and Brain Research Centre, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Science 312:924-7. 2006
  8. pmc Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions
    Izumi Maezawa
    M I N D Medical Investigation of Neurodevelopmental Disorders Institute, University of California Davis Medical Center, Sacramento, California 95817, USA
    J Neurosci 29:5051-61. 2009
  9. pmc Connexin channel permeability to cytoplasmic molecules
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, Newark, NJ 07103, USA
    Prog Biophys Mol Biol 94:120-43. 2007
  10. ncbi Structure of the connexin 26 gap junction channel at 3.5 A resolution
    Shoji Maeda
    Institute for Protein Research, Osaka University, OLABB, 6 2 3, Furuedai, Suita, Osaka 565 0874, Japan
    Nature 458:597-602. 2009

Detail Information

Publications348 found, 100 shown here

  1. ncbi Plasma membrane channels formed by connexins: their regulation and functions
    Juan C Saez
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Alameda 340, Santiago, Chile
    Physiol Rev 83:1359-400. 2003
    ..The physiological role of gap junctions in several tissues has been elucidated by the discovery of mutant connexins associated with genetic diseases ..
  2. ncbi Tumor necrosis factor-alpha induces neurotoxicity via glutamate release from hemichannels of activated microglia in an autocrine manner
    Hideyuki Takeuchi
    Department of Neuroimmunology, Research Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Japan
    J Biol Chem 281:21362-8. 2006
    ..These drugs may give us a new therapeutic strategy against neurodegenerative diseases with minimum adverse side effects...
  3. ncbi Pannexin membrane channels are mechanosensitive conduits for ATP
    Li Bao
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 572:65-8. 2004
    ..They have a wide expression spectrum, they are of large conductance and permeant for ATP, and they are mechanosensitive. Hence, pannexins are candidates for the release of ATP to the extracellular space upon mechanical stress...
  4. ncbi An imbalancing act: gap junctions reduce the backward motor circuit activity to bias C. elegans for forward locomotion
    Taizo Kawano
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON M5G1X5, Canada
    Neuron 72:572-86. 2011
    ..elegans for forward locomotion. This study demonstrates that imbalanced motoneuron activity underlies directional movement and establishes gap junctions as critical modulators of the properties and outputs of neural circuits.
  5. pmc Gap junctions
    Daniel A Goodenough
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Biol 1:a002576. 2009
    b>Gap junctions are aggregates of intercellular channels that permit direct cell-cell transfer of ions and small molecules...
  6. ncbi Gap junctions and the connexin protein family
    Goran Sohl
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, 53117 Bonn, Germany
    Cardiovasc Res 62:228-32. 2004
    b>Gap junctions (Gj) form conduits between adjacent cells that are composed of connexin (Cx) protein subunits and allow direct intercellular communication...
  7. ncbi Ischemia opens neuronal gap junction hemichannels
    Roger J Thompson
    Department of Psychiatry and Brain Research Centre, University of British Columbia, Vancouver, British Columbia V6T 2B5, Canada
    Science 312:924-7. 2006
    ..We show that ischemic-like conditions [O(2)/glucose deprivation (OGD)] open hemichannels, or half gap junctions, in neurons...
  8. pmc Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions
    Izumi Maezawa
    M I N D Medical Investigation of Neurodevelopmental Disorders Institute, University of California Davis Medical Center, Sacramento, California 95817, USA
    J Neurosci 29:5051-61. 2009
    ..Our results suggest that astrocytes are viable therapeutic targets for RTT and perhaps regressive forms of autism...
  9. pmc Connexin channel permeability to cytoplasmic molecules
    Andrew L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School of UMDNJ, Newark, NJ 07103, USA
    Prog Biophys Mol Biol 94:120-43. 2007
    ..At this time, the nature of those interactions is unclear. One hopes that with more detailed permeability and structural information, the specific molecular mechanisms of the selectivity can be elucidated...
  10. ncbi Structure of the connexin 26 gap junction channel at 3.5 A resolution
    Shoji Maeda
    Institute for Protein Research, Osaka University, OLABB, 6 2 3, Furuedai, Suita, Osaka 565 0874, Japan
    Nature 458:597-602. 2009
    b>Gap junctions consist of arrays of intercellular channels between adjacent cells that permit the exchange of ions and small molecules...
  11. pmc Remodelling of gap junctions and connexin expression in diseased myocardium
    Nicholas J Severs
    National Heart and Lung Institute, Imperial College London, Dovehouse Street, London SW3 6LY, UK
    Cardiovasc Res 80:9-19. 2008
    b>Gap junctions form the cell-to-cell pathways for propagation of the precisely orchestrated patterns of current flow that govern the regular rhythm of the healthy heart...
  12. ncbi Gap junction adhesion is necessary for radial migration in the neocortex
    Laura A B Elias
    Neuroscience Graduate Program, University of California San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA
    Nature 448:901-7. 2007
    ..Unexpectedly, gap junctions do not mediate neuronal migration by acting in the classical manner to provide an aqueous channel for cell-..
  13. pmc Connexin43 phosphorylation: structural changes and biological effects
    Joell L Solan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800 Box 19024, Seattle, WA 98109, USA
    Biochem J 419:261-72. 2009
    Vertebrate gap junctions, composed of proteins from the connexin gene family, play critical roles in embryonic development, co-ordinated contraction of excitable cells, tissue homoeostasis, normal cell growth and differentiation...
  14. pmc Metabolic inhibition induces opening of unapposed connexin 43 gap junction hemichannels and reduces gap junctional communication in cortical astrocytes in culture
    Jorge E Contreras
    Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago 340, Chile
    Proc Natl Acad Sci U S A 99:495-500. 2002
    Rat cortical astrocytes in pure culture are functionally coupled to neighboring cells via connexin (Cx) 43 gap junctions under ordinary conditions...
  15. pmc The effects of connexin phosphorylation on gap junctional communication
    Paul D Lampe
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North DE 320, Box 19024, Seattle, WA 98109, USA
    Int J Biochem Cell Biol 36:1171-86. 2004
    b>Gap junctions are specialized membrane domains composed of collections of channels that directly connect neighboring cells providing for the cell-to-cell diffusion of small molecules, including ions, amino acids, nucleotides, and second ..
  16. ncbi Role of olivary electrical coupling in cerebellar motor learning
    Ruben S Van Der Giessen
    Department of Neuroscience, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Neuron 58:599-612. 2008
    ..at both the cellular and systems level, suggest that electrotonic coupling among olivary neurons by gap junctions is essential for proper timing of their action potentials and thereby for learning-dependent timing in ..
  17. ncbi Role of gap junctions in the propagation of the cardiac action potential
    Stephan Rohr
    Department of Physiology, University of Bern, Buhlplatz 5, CH 3012 Bern, Switzerland
    Cardiovasc Res 62:309-22. 2004
    b>Gap junctions play a pivotal role for the velocity and the safety of impulse propagation in cardiac tissue...
  18. ncbi The mammalian pannexin family is homologous to the invertebrate innexin gap junction proteins
    Ancha Baranova
    Vavilov Institute of General Genetics RAS, Gubkina str, 3 GSP 1, Moscow 119991, Russia
    Genomics 83:706-16. 2004
    ..In silico evaluation of Panx3 expression predicts gene expression in osteoblasts and synovial fibroblasts. The apparent conservation of pannexins between species merits further investigation...
  19. pmc Neuroinflammation leads to region-dependent alterations in astrocyte gap junction communication and hemichannel activity
    Nikolay Karpuk
    Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Neurosci 31:414-25. 2011
    ..This relationship may extend to other CNS diseases typified with an inflammatory component...
  20. ncbi Structural and functional diversity of connexin genes in the mouse and human genome
    Klaus Willecke
    Institut fur Genetik, Abteilung Molekulargenetik, Universitat Bonn, Germany
    Biol Chem 383:725-37. 2002
    b>Gap junctions are clustered channels between contacting cells through which direct intercellular communication via diffusion of ions and metabolites can occur...
  21. pmc Interactions between ankyrin-G, Plakophilin-2, and Connexin43 at the cardiac intercalated disc
    Priscila Y Sato
    Division of Cardiovascular Medicine, University of Michigan Medical School, Ann Arbor, MI, USA
    Circ Res 109:193-201. 2011
    ..of the intercalated disc defined 3 structures, all of them involved in cell-cell communication: desmosomes, gap junctions, and adherens junctions...
  22. ncbi Specific gap junctions enhance the neuronal vulnerability to brain traumatic injury
    Marina V Frantseva
    The Hospital for Sick Children, Toronto, Ontario, M5G 1X8, Canada
    J Neurosci 22:644-53. 2002
    ..damage focuses on synaptic or ionic mechanisms, the possible role of direct intercellular communication via gap junctions has remained unexplored...
  23. ncbi Emerging issues of connexin channels: biophysics fills the gap
    A L Harris
    Department of Pharmacology and Physiology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, 185 South Orange Ave, University Heights, Newark, NJ 07103, USA
    Q Rev Biophys 34:325-472. 2001
    ..However, the relation between these two mechanisms are unexplored, as are specific mechanisms of direct endogenous regulation of connexin channel activity. (ABSTRACT TRUNCATED)..
  24. ncbi Gap junctions and connexin-interacting proteins
    Ben N G Giepmans
    Department of Neurosciences, The National Center of Microscopy and Imaging Research, University of California San Diego, School of Medicine, 1000 BSB, 9500 Gilman Drive, MC0608, La Jolla, CA 92093 0608, USA
    Cardiovasc Res 62:233-45. 2004
    b>Gap junctions form channels between adjacent cells. The core proteins of these channels are the connexins...
  25. ncbi Block of inferior olive gap junctional coupling decreases Purkinje cell complex spike synchrony and rhythmicity
    Timothy A Blenkinsop
    Department of Physiology and Neuroscience, School of Medicine, New York University, New York, New York 10016, USA
    J Neurosci 26:1739-48. 2006
    Inferior olivary (IO) neurons are electrotonically coupled by gap junctions. This coupling is thought to underlie synchronous complex spike (CS) activity generated by the olivocerebellar system in Purkinje cells, and also has been ..
  26. pmc Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40V and G45E mutations that cause keratitis ichthyosis deafness syndrome
    Helmuth A Sánchez
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Gen Physiol 136:47-62. 2010
    ..A40V produces leaky hemichannels, whereas G45E provides a route for excessive entry of Ca(2+). These aberrant properties, alone or in combination, can severely compromise cell integrity and lead to increased cell death...
  27. ncbi Role of gap junctions in synchronized neuronal oscillations in the inferior olive
    Elena Leznik
    Department of Physiology and Neuroscience, New York University Medical School, NY 10016, USA
    J Neurophysiol 94:2447-56. 2005
    Inferior olivary (IO) neurons are electrically coupled through gap junctions and generate synchronous subthreshold oscillations of their membrane potential at a frequency of 1-10 Hz...
  28. pmc Oxidative stress, lens gap junctions, and cataracts
    Viviana M Berthoud
    Department of Pediatrics, University of Chicago, Chicago, Illinois 60637, USA
    Antioxid Redox Signal 11:339-53. 2009
    ..Maintenance of transparency and homeostasis of the avascular lens depend on an extensive network of gap junctions. Communication through gap junction channels allows intercellular passage of molecules (up to 1 kDa) including ..
  29. pmc Biological role of connexin intercellular channels and hemichannels
    Rekha Kar
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229 3900, USA
    Arch Biochem Biophys 524:2-15. 2012
    b>Gap junctions (GJ) and hemichannels (HC) formed from the protein subunits called connexins are transmembrane conduits for the exchange of small molecules and ions...
  30. ncbi Somatic mutations in the connexin 40 gene (GJA5) in atrial fibrillation
    Michael H Gollob
    Arrhythmia Research Laboratory and Division of Cardiology, Department of Medicine, University of Ottawa Heart Institute, Ottawa, Canada
    N Engl J Med 354:2677-88. 2006
    ..We hypothesized that idiopathic atrial fibrillation has a genetic basis and that tissue-specific mutations in GJA5, the gene encoding connexin 40, may predispose the atria to fibrillation...
  31. ncbi The impact of astrocytic gap junctional coupling on potassium buffering in the hippocampus
    Anke Wallraff
    Department of Experimental Neurobiology, Neurosurgery, University of Bonn, 53105 Bonn, Germany
    J Neurosci 26:5438-47. 2006
    Astrocytic gap junctions have been suggested to contribute to spatial buffering of potassium in the brain. Direct evidence has been difficult to gather because of the lack of astrocyte-specific gap junction blockers...
  32. ncbi Key functions for gap junctions in skin and hearing
    Claire A Scott
    Centre for Cutaneous Research, The Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, UK
    Biochem J 438:245-54. 2011
    Cx (connexin) proteins are components of gap junctions which are aqueous pores that allow intercellular exchange of ions and small molecules. Mutations in Cx genes are linked to a range of human disorders...
  33. pmc Zonula occludens-1 alters connexin43 gap junction size and organization by influencing channel accretion
    Andrew W Hunter
    Department of Cell Biology and Anatomy, Cardiovascular Developmental Biology Center, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Biol Cell 16:5686-98. 2005
    ..We propose that ZO-1 controls the rate of Cx43 channel accretion at GJ peripheries, which, in conjunction with the rate of GJ turnover, regulates GJ size and distribution...
  34. ncbi Connexin 43 hemichannels contribute to the propagation of apoptotic cell death in a rat C6 glioma cell model
    E Decrock
    Department of Basic Medical Sciences Physiology group, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Cell Death Differ 16:151-63. 2009
    b>Gap junctions (GJs) have been demonstrated to communicate cell death signals from apoptotic to healthy cells, thereby spatially extending apoptosis...
  35. ncbi Acute downregulation of connexin43 at wound sites leads to a reduced inflammatory response, enhanced keratinocyte proliferation and wound fibroblast migration
    Ryoichi Mori
    Department of Physiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK
    J Cell Sci 119:5193-203. 2006
    ....
  36. ncbi The lens circulation
    Richard T Mathias
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York, NY 11794 8661, USA
    J Membr Biol 216:1-16. 2007
    ..This review covers the membrane transport systems that are believed to generate and direct this internal circulatory system...
  37. pmc Carbenoxolone blockade of neuronal network activity in culture is not mediated by an action on gap junctions
    N Rouach
    Department of Neurobiology, The Weizmann Institute, Rehovot, 76100 Israel
    J Physiol 553:729-45. 2003
    Spontaneous activity in the central nervous system is strongly suppressed by blockers of gap junctions (GJs), suggesting that GJs contribute to network activity...
  38. pmc Gap junction coupling and calcium waves in the pancreatic islet
    Richard K P Benninger
    Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA
    Biophys J 95:5048-61. 2008
    ..The resulting quantitative agreement between the data and analytical models of islet connectivity, using only a single free parameter, reveals the mechanistic underpinnings of the multicellular behavior of the islet...
  39. ncbi Cx43 hemichannels and gap junction channels in astrocytes are regulated oppositely by proinflammatory cytokines released from activated microglia
    Mauricio A Retamal
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago 6513492, Chile
    J Neurosci 27:13781-92. 2007
    ..including interleukin-1beta and tumor necrosis factor-alpha, that reduce intercellular communication via gap junctions. Now, we demonstrate that either conditioned medium harvested from activated microglia or a mixture of these ..
  40. ncbi Gap junctions: structure and function (Review)
    W Howard Evans
    Department of Medical Biochemistry and Diagnostic Radiology, University of Wales College of Medicine, Cardiff, UK
    Mol Membr Biol 19:121-36. 2002
    b>Gap junctions are plasma membrane spatial microdomains constructed of assemblies of channel proteins called connexins in vertebrates and innexins in invertebrates...
  41. pmc Cell-to-cell communication in intact taste buds through ATP signalling from pannexin 1 gap junction hemichannels
    Robin Dando
    Miller School of Medicine, University of Miami Physiology and Biophysics and Program in Neuroscience, 1600 NW 10th Ave, Miami, FL 33136, USA
    J Physiol 587:5899-906. 2009
    ..These findings are consistent with the model for peripheral signal processing via ATP and pannexin 1 hemichannels in mammalian taste buds...
  42. pmc Connexin-specific cell-to-cell transfer of short interfering RNA by gap junctions
    V Valiunas
    Department of Physiology and Biophysics, SUNY at Stony Brook, NY 11794, USA
    J Physiol 568:459-68. 2005
    The purpose of this study was to determine whether oligonucleotides the size of siRNA are permeable to gap junctions and whether a specific siRNA for DNA polymerase beta (pol beta) can move from one cell to another via gap junctions, ..
  43. pmc Life cycle of connexins in health and disease
    Dale W Laird
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochem J 394:527-43. 2006
    ..This review will assess the current understanding of wild-type and selected disease-linked mutant connexin transport through the secretory pathway, gap-junction assembly at the cell surface, internalization and degradation...
  44. pmc Pannexins, a family of gap junction proteins expressed in brain
    Roberto Bruzzone
    Department of Clinical Neurobiology, Interdisciplinary Center for Neurosciences, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 100:13644-9. 2003
    ..Px1 versus heteromeric Px1/Px2 channels and the different expression patterns of Px1 and Px2 in the brain indicate that pannexins form cell type-specific gap junctions with distinct properties that may subserve different functions.
  45. ncbi Mutations in connexin genes and disease
    Anna Pfenniger
    Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland
    Eur J Clin Invest 41:103-16. 2011
    ..Knowledge on this family of proteins can be gained by making an inventory of mutations and associated diseases in human...
  46. ncbi Cross-presentation by intercellular peptide transfer through gap junctions
    Joost Neijssen
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nature 434:83-8. 2005
    ..can be transferred directly from the cytoplasm of one cell into the cytoplasm of its neighbour through gap junctions. Here we show that peptides with a relative molecular mass of up to approximately 1,800 diffuse ..
  47. ncbi Lens gap junctions in growth, differentiation, and homeostasis
    Richard T Mathias
    Department of Physiology and Biophysics, SUNY at Stony Brook, Stony Brook, New York 11794 8661, USA
    Physiol Rev 90:179-206. 2010
    The cells of most mammalian organs are connected by groups of cell-to-cell channels called gap junctions. Gap junction channels are made from the connexin (Cx) family of proteins...
  48. ncbi Inhibition of proliferation of a hepatoma cell line by fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication
    Cheng Ling Liu
    Department of Food Science, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 402, Taiwan
    Chem Biol Interact 182:165-72. 2009
    ..The enhanced GJIC may be responsible for the increase of the intracellular calcium level, which then causes cell cycle arrest and apoptosis...
  49. ncbi Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins
    Silvia Penuela
    Department of Anatomy and Cell Biology, University of Western Ontario, London, ON, N6A 5C1, Canada
    J Cell Sci 120:3772-83. 2007
    ..Collectively, these studies show that Panx1 and Panx3 have many characteristics that are distinct from Cx43 and that these proteins probably play an important biological role as single membrane channels...
  50. ncbi The role of gap junctions in Caenorhabditis elegans oocyte maturation and fertilization
    Scott J Whitten
    Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Dev Biol 301:432-46. 2007
    We have investigated the role of gap junctions in Caenorhabditis elegans oocyte maturation and fertilization. Gap junctions are observed between oocytes and the surrounding ovarian sheath cells in wild-type gonads...
  51. ncbi Functional hemichannels in astrocytes: a novel mechanism of glutamate release
    Zu Cheng Ye
    Department of Neurology, University of Washington School of Medicine, Seattle, Washington 98195 6465, USA
    J Neurosci 23:3588-96. 2003
    ..Our results indicated that astrocyte hemichannels could influence CNS levels of extracellular glutamate with implications for normal and pathological brain function...
  52. ncbi Role of connexin 43 in ischemic preconditioning does not involve intercellular communication through gap junctions
    X Li
    Institut fur Pathophysiologie, Zentrum fur Innere Medizin, Universitatsklinikum Essen, Hufelandstrasse 55, Essen 45122, Germany
    J Mol Cell Cardiol 36:161-3. 2004
    ..It is, however, unclear whether the important role of Cx 43 relates to intercellular communication through gap junctions or its function in volume homeostasis...
  53. ncbi Involvement of gap junctions in the manifestation and control of the duration of seizures in rats in vivo
    Zita Gajda
    Department of Comparative Physiology, University of Szeged, Közép fasor 52, H 6726 Szeged, Hungary
    Epilepsia 44:1596-600. 2003
    The possible role of gap junctions in the manifestation and control of the duration of seizures was tested on the 4-aminopyridine-induced epilepsy model in rats in vivo, by using electrophysiologic, pharmacologic, and molecular biologic ..
  54. pmc Connexin mutation that causes dominant congenital cataracts inhibits gap junctions, but not hemichannels, in a dominant negative manner
    Eric A Banks
    Department of Biochemistry, University of Texas Health Science Center, San Antonio, TX 78229, USA
    J Cell Sci 122:378-88. 2009
    ..infection similarly failed to induce dye coupling, and prevented wild-type chCx50 from forming functional gap junctions. In contrast to its effect on gap junctions, the E48K mutation has no effect on hemichannel activity when ..
  55. ncbi Gap junction channels formed by coexpressed connexin40 and connexin43
    V Valiunas
    Department of Physiology and Biophysics, State University of New York, Stony Brook, New York 11794, USA
    Am J Physiol Heart Circ Physiol 281:H1675-89. 2001
    ..The dual whole cell patch-clamp method was used to compare the gating properties of gap junctions in HeLa Cx43/Cx40 cells with homotypic (Cx40-Cx40 and Cx43-Cx43) and heterotypic (Cx40-Cx43) gap junctions...
  56. ncbi Diversity and properties of connexin gap junction channels
    Mindaugas Rackauskas
    Institute of Cardiology, Kaunas University of Medicine, Lithuania
    Medicina (Kaunas) 46:1-12. 2010
    ....
  57. ncbi The gap junction proteome and its relationship to disease
    Dale W Laird
    Department of Anatomy and Cell Biology, and Physiology and Pharmacology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Trends Cell Biol 20:92-101. 2010
    ....
  58. ncbi Innexins: members of an evolutionarily conserved family of gap-junction proteins
    Pauline Phelan
    Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK
    Biochim Biophys Acta 1711:225-45. 2005
    b>Gap junctions are clusters of intercellular channels that provide cells, in all metazoan organisms, with a means of communicating directly with their neighbours...
  59. ncbi Defective gap junctional intercellular communication in the carcinogenic process
    Marc Mesnil
    Equipe Interactions et Communications Cellulaires, Institut de Physiologie et Biologie Cellulaires, CNRS UMR 6187, Universite de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France
    Biochim Biophys Acta 1719:125-45. 2005
    b>Gap junctions are membrane structures made of intercellular channels which permit the diffusion from cytoplasm to cytoplasm of small hydrophilic molecules...
  60. pmc pH-dependent modulation of voltage gating in connexin45 homotypic and connexin45/connexin43 heterotypic gap junctions
    Nicolas Palacios-Prado
    Dominick P Purpura Department of Neuroscience and Department of Anesthesiology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 107:9897-902. 2010
    ..7, between the pK(a)s of Cx45 and Cx43-EGFP (approximately 6.5) homotypic GJs. In summary, pH(i) significantly modulates junctional conductance of Cx45 by affecting both V(j) gating and number of functional channels...
  61. ncbi Connexins and gap junctions in mammary gland development and breast cancer progression
    Elizabeth McLachlan
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada
    J Membr Biol 218:107-21. 2007
    ..In breast cancer, connexins generally act as tumor suppressors in the primary tumor; however, in advanced breast tumors, connexins appear to act as both context-dependent tumor suppressors and facilitators of disease progression...
  62. pmc Modulation of brain hemichannels and gap junction channels by pro-inflammatory agents and their possible role in neurodegeneration
    Juan A Orellana
    Departamento de Ciencias Fisiologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Antioxid Redox Signal 11:369-99. 2009
    ..These changes are likely to occur in diverse cell types of the CNS and contribute to neurodegeneration during inflammatory process...
  63. ncbi Osmotic forces and gap junctions in spreading depression: a computational model
    B E Shapiro
    Machine Learning Systems Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena 91109, USA
    J Comput Neurosci 10:99-120. 2001
    ..model of spreading depression (SD), ionic movement through a neuronal syncytium of cells connected by gap junctions is described electrodiffusively...
  64. ncbi Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase
    B J Warn-Cramer
    Molecular Carcinogenesis Section, Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Biol Chem 273:9188-96. 1998
    ..Studies with connexin-43 mutants suggest that MAP kinase phosphorylation at one or more of the tandem Ser279/Ser282 sites is sufficient to disrupt gap junctional intercellular communication...
  65. pmc Internalized gap junctions are degraded by autophagy
    John T Fong
    Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA
    Autophagy 8:794-811. 2012
    Direct intercellular communication mediated by gap junctions (GJs) is a hallmark of normal cell and tissue physiology. In addition, GJs significantly contribute to physical cell-cell adhesion...
  66. ncbi Cell-cell communication in carcinogenesis
    J E Trosko
    Department of Pediatrics and Human Development, Michigan State University, East Lansing, Michigan 48824, USA
    Front Biosci 3:d208-36. 1998
    ..From this background, implications to cancer prevention and cancer therapy are generated...
  67. ncbi Connexins and gap junctions of astrocytes and oligodendrocytes in the CNS
    J I Nagy
    Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    Brain Res Brain Res Rev 32:29-44. 2000
    This review article summarizes early and recent literature on the structure, distribution and composition of gap junctions between astrocytes and oligodendrocytes, and the differential expression of glial connexins in adult and ..
  68. ncbi Gap junctions and the propagation of cell survival and cell death signals
    D V Krysko
    Department of Human Anatomy, Embryology, Histology and Medical Physics, Ghent University, 9000 Ghent, Belgium
    Apoptosis 10:459-69. 2005
    b>Gap junctions are a unique type of intercellular channels that connect the cytoplasm of adjoining cells...
  69. ncbi Mutations of the Connexin43 gap-junction gene in patients with heart malformations and defects of laterality
    S H Britz-Cunningham
    Department of Physiology, Loma Linda University, CA 92354, USA
    N Engl J Med 332:1323-9. 1995
    b>Gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development...
  70. ncbi Pharmacology of gap junctions. New pharmacological targets for treatment of arrhythmia, seizure and cancer?
    Aida Salameh
    Clinic I for Internal Medicine, Department of Cardiology, University of Leipzig, Johannisallee 32, 04103 Leipzig, Germany
    Biochim Biophys Acta 1719:36-58. 2005
    ..Thus, depending on the target disease opening or closure of gap junctions may be of interest, or alteration of connexin expression...
  71. ncbi Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease)
    Starr R Kaplan
    Department of Pathology and Center for Cardiovascular Research, Washington University School of Medicine, St Louis, Missouri 63100, USA
    Heart Rhythm 1:3-11. 2004
    ..that defective interactions between adhesion junctions and the cytoskeleton caused by the plakoglobin mutation in Naxos disease lead to remodeling of gap junctions and altered expression of the major gap junction protein, connexin43.
  72. ncbi Macrophage-induced neurotoxicity is mediated by glutamate and attenuated by glutaminase inhibitors and gap junction inhibitors
    Izumi Yawata
    Department of Neuroimmunology, Institute of Environmental Medicine, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8601, Japan
    Life Sci 82:1111-6. 2008
    ..that macrophages produce glutamate via glutaminase from extracelluar glutamine, and release it through gap junctions. This study demonstrated that a similar machinery is operating in macrophages as well, and DON and CBX that ..
  73. ncbi Gap junctions or hemichannel-dependent and independent roles of connexins in cataractogenesis and lens development
    J X Jiang
    Department of Biochemistry, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78229 3900, USA
    Curr Mol Med 10:851-63. 2010
    ..Recent advances in basic research of lens connexins and the discoveries of clinical disorders as a result of lens connexin dysfunctions are summarized and discussed here...
  74. pmc Gap junctions mediate human immunodeficiency virus-bystander killing in astrocytes
    Eliseo A Eugenin
    Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, New York 10461, USA
    J Neurosci 27:12844-50. 2007
    ..In this report, we characterize a novel role of gap junctions in transmitting and thereby amplifying toxic signals originating from HIV-infected astrocytes that trigger ..
  75. ncbi Gap junctions and connexin expression in the normal and pathological central nervous system
    N Rouach
    INSERM U114, College de France, 11, place Marcelin Berthelot 75005, Paris, France
    Biol Cell 94:457-75. 2002
    b>Gap junctions are widely expressed in the various cell types of the central nervous system...
  76. ncbi Inhibition of cytokine-induced connexin43 hemichannel activity in astrocytes is neuroprotective
    Nicolas Froger
    Inserm U840, College de France, 11 place Marcelin Berthelot, 75231 Paris, Cedex 05, France
    Mol Cell Neurosci 45:37-46. 2010
    ..Altogether, these data demonstrate that inflammation-induced astroglial hemichannel activation plays a critical role in neuronal death and suggest a neuroprotective role of connexin43 hemichannel blockade...
  77. ncbi Cell death in retinitis pigmentosa: gap junctions and the 'bystander' effect
    Harris Ripps
    Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, Chicago, IL 60612, USA
    Exp Eye Res 74:327-36. 2002
    ..of the disease from dying rods to genetically normal cones is a form of 'bystander' effect, mediated by the gap junctions that exist between these photoreceptor subtypes...
  78. pmc Cell-specific expression of connexins and evidence of restricted gap junctional coupling between glial cells and between neurons
    J E Rash
    Department of Anatomy, Colorado State University, Fort Collins, Colorado 80523, USA
    J Neurosci 21:1983-2000. 2001
    The transmembrane connexin proteins of gap junctions link extracellularly to form channels for cell-to-cell exchange of ions and small molecules...
  79. ncbi Molecular reorganization of Cx43, Zo-1 and Src complexes during the endocytosis of gap junction plaques in response to a non-genomic carcinogen
    Jerome Gilleron
    INSERM U 895, Team 5 Physiopathologic control of germ cell proliferation genomic and non genomic mechanisms, Universite Paris Descartes, 45 rue des Saint Peres, 75006, Paris, France
    J Cell Sci 121:4069-78. 2008
    ..A number of connexin (Cx)-binding proteins are known to be involved in endocytic internalization of gap junctions. Here, we analyzed the discrete molecular interactions that occur between Src, ZO-1 and Cx43 during Cx43 ..
  80. ncbi Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration
    Cima Cina
    Department of Cellular and Physiological Sciences, The Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
    J Neurosci 29:2009-21. 2009
    ..It has been shown previously that gap junctions are needed for this migration to occur properly, but the precise mechanism responsible is still in question...
  81. ncbi Mechanically stimulated osteocytes regulate osteoblastic activity via gap junctions
    A F Taylor
    Department of Orthopaedics and Rehabilitation, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA
    Am J Physiol Cell Physiol 292:C545-52. 2007
    ....
  82. pmc Human mesenchymal stem cells make cardiac connexins and form functional gap junctions
    Virginijus Valiunas
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, NY 11794 8661, USA
    J Physiol 555:617-26. 2004
    ..repair or delivery system provided that they communicate with target cells such as cardiac myocytes via gap junctions. Immunostaining revealed typical punctate staining for Cx43 and Cx40 along regions of intimate cell-to-cell ..
  83. ncbi Connexin phosphorylation as a regulatory event linked to gap junction internalization and degradation
    Dale W Laird
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada N6A 5C1
    Biochim Biophys Acta 1711:172-82. 2005
    ..is regulated by connexin biosynthesis, transport and assembly as well as the formation and removal of gap junctions from the cell surface...
  84. ncbi Connexins and their channels in cell growth and cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Cell Signal 18:592-600. 2006
    Direct communication between cells, mediated by gap junctions, is nowadays considered as an indispensable mechanism in the maintenance of cellular homeostasis...
  85. ncbi Beyond the gap: functions of unpaired connexon channels
    Daniel A Goodenough
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Nat Rev Mol Cell Biol 4:285-94. 2003
    b>Gap junctions consist of intercellular channels that connect the cytoplasm of adjacent cells directly and allow the exchange of small molecules...
  86. pmc ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear
    Fabio Anselmi
    Foundation for Advanced Biomedical Research, Venetian Institute of Molecular Medicine, 35129 Padua, Italy
    Proc Natl Acad Sci U S A 105:18770-5. 2008
    ....
  87. ncbi Gap junctions and ovarian folliculogenesis
    Gerald M Kidder
    Department of Physiology, The University of Western Ontario, London, N6A 5C1, Canada
    Reproduction 123:613-20. 2002
    b>Gap junctions are collections of intercellular membrane channels that allow adjacent cells to share small molecules (< 1 kDa). Gap junction channels are composed of connexins, a homologous family of more than 20 proteins...
  88. ncbi Phosphorylation of connexin43 induced by Src: regulation of gap junctional communication between transformed cells
    Madhuri Pahujaa
    Department of Cell Biology, University of Medicine and Dentistry of New Jersey, Science Center, 2 Medical Center Dr, Stratford, NJ 08084, USA
    Exp Cell Res 313:4083-90. 2007
    ..Indeed, disruption of gap junctions by Src appears to be complex. It has become clear that Src can affect Cx43 activity by multiple mechanisms...
  89. pmc New roles for astrocytes: gap junction hemichannels have something to communicate
    Michael V L Bennett
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Neurosci 26:610-7. 2003
    b>Gap junctions are clusters of aqueous channels that connect the cytoplasm of adjoining cells. Each cell contributes a hemichannel, or connexon, to each cell-cell channel...
  90. pmc Gap junctions in the control of vascular function
    Xavier F Figueroa
    Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago, Chile
    Antioxid Redox Signal 11:251-66. 2009
    Direct intercellular communication via gap junctions is critical in the control and coordination of vascular function...
  91. ncbi Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect
    Edouard I Azzam
    Department of Radiology, UMDNJ, New Jersey Medical School, Newark, NJ 07103
    Oncogene 22:7050-7. 2003
    ..Our current knowledge of the biochemical and molecular events involved in the latter two processes is reviewed in this article...
  92. ncbi Functional connexin "hemichannels": a critical appraisal
    David C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Glia 54:758-73. 2006
    ..We review the concept of "hemichannels", summarize properties that might be expected of half gap junctions and evaluate the extent to which the properties of presumptive "hemichannels" match expectations...
  93. ncbi The role of connexins in controlling cell growth and gene expression
    Elissavet Kardami
    Institute of Cardiovascular Sciences, University of Manitoba and St Boniface Research Centre, Winnipeg, Man, Canada
    Prog Biophys Mol Biol 94:245-64. 2007
    ..Finally, Section 4 will pay tribute to the rapidly emerging realization that connexins such as Cx43 and Cx32 exert important and extensive effects on gene expression, particularly those genes linked to growth regulation...
  94. pmc Gap junctions between striatal fast-spiking interneurons regulate spiking activity and synchronization as a function of cortical activity
    Johannes Hjorth
    Computational Biology, School of Computer Science and Communication, Royal Institute of Technology, AlbaNova University Centre, 106 91 Stockholm, Sweden
    J Neurosci 29:5276-86. 2009
    Striatal fast-spiking (FS) interneurons are interconnected by gap junctions into sparsely connected networks...
  95. ncbi A possible role for gap junctions in generation of very fast EEG oscillations preceding the onset of, and perhaps initiating, seizures
    R D Traub
    Department of Pharmacology, University of Birmingham School of Medicine, Vincent Drive, Edgbaston, Birmingham B15 2TT, UK
    Epilepsia 42:153-70. 2001
    ..These oscillations are important, as they may play a causal role in the initiation of seizures...
  96. ncbi Gap junctions between interneuron dendrites can enhance synchrony of gamma oscillations in distributed networks
    R D Traub
    Department of Pharmacology, University of Birmingham School of Medicine, Edgbaston, Birmingham B15 2TT, United Kingdom
    J Neurosci 21:9478-86. 2001
    ..gamma oscillations, provided that sufficiently many pyramidal neurons are active; we also suggested that gap junctions, between the axons of principal neurons, could contribute to the long-range synchrony of gamma oscillations ..
  97. pmc Staphylococcus aureus-derived peptidoglycan induces Cx43 expression and functional gap junction intercellular communication in microglia
    Sarita Garg
    Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    J Neurochem 95:475-83. 2005
    b>Gap junctions serve as intercellular conduits that allow the exchange of small molecular weight molecules (up to 1 kDa) including ions, metabolic precursors and second messengers...
  98. ncbi A neuroprotective role for gap junctions
    C C Naus
    Department of Anatomy and Cell Biology, The University of Western Ontario, London, Canada
    Cell Commun Adhes 8:325-8. 2001
    ..In astrocytes, gap junctions are composed primarily of the channel protein, connexin43 (Cx43), and provide a substrate for formation of a ..
  99. ncbi Connexin-related signaling in cell death: to live or let die?
    E Decrock
    Department of Basic Medical Sciences Physiology group, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
    Cell Death Differ 16:524-36. 2009
    ..The latter is provided by arrays of channels consisting of connexin proteins, with gap junctions directly connecting the cytoplasm of neighboring cells and hemichannels positioned as pores that link the ..
  100. ncbi Blocked gap junctional coupling increases glutamate-induced neurotoxicity in neuron-astrocyte co-cultures
    Mark A Ozog
    Department of Anatomy and Cell Biology, The University of Western Ontario, London, Canada
    J Neuropathol Exp Neurol 61:132-41. 2002
    ..e. ischemia). To examine this neuroprotective role of gap junctions, we employed gap junctional blockers to neuronal and astrocytic co-cultures during exposure to a high ..
  101. ncbi Gap junctional channels are parts of multiprotein complexes
    Jean Claude Hervé
    Institut de Physiologie et Biologie Cellulaires, Universite de Poitiers, CNRS, Poitiers, France
    Biochim Biophys Acta 1818:1844-65. 2012
    ..This article is part of a Special Issue entitled: The Communicating junctions, composition, structure and functions...

Research Grants77

  1. Connexins in Neuronal and Glial Gap Junctions in the Central Nervous System
    John E Rash; Fiscal Year: 2012
    DESCRIPTION (provided by applicant): Gap junctions are microscopic intercellular junctions that provide for direct intercellular ionic, electrical, and metabolic coupling between nerve cells in the brain, retina, and spinal cord...
  2. Cx43 phosphorylation modulates Kras mediated pancreas cancer progression
    Paul D Lampe; Fiscal Year: 2012
    ..This proposal focuses on how these events interplay in vivo to affect carcinogenesis of the pancreas. Gap junctions (GJ) are specialized membrane domains that contain channels that allow exchange of small molecules (<1000 ..
  3. Mechanisms of Ventricular Tachycardia in Lipotoxic Cardiomyopathy
    JOHN PEARCE MORROW; Fiscal Year: 2013
    ..application is that increased cardiac myocyte lipid content leads to abnormal regulation of ion channels and gap junctions, promoting arrhythmia...
  4. VOLUME REGULATION IN NORMAL AND CATARACTOUS LENSES
    Richard T Mathias; Fiscal Year: 2013
    ..The circulation depends on fiber cell gap junctions to conduct intracellular fluxes of ions and perhaps fluid, which flow from the center to surface of the lens...
  5. Calcium wave propagation along microvascular endothelial tubes
    MATTHEW JOHN SOCHA; Fiscal Year: 2012
    ..Preliminary studies with dye transfer confirm that ECs throughout the tube are well-coupled through gap junctions and generate robust Ca+2 waves in response to ACh that can propagate more than 500 5m along the tube...
  6. CONTROL OF MEIOSIS AND GERMLINE PROLIFERATION
    DAVID IRWIN GREENSTEIN; Fiscal Year: 2012
    ..about their underlying mechanistic basis, hence we will: 1) Define the molecular composition of sheath/oocyte gap junctions;2) Test the hypothesis that MSP signaling targets inhibitory sheath/oocyte gap junctions to promote meiotic ..
  7. Connexin 43 in Bone Remodeling
    Roberto Civitelli; Fiscal Year: 2013
    ..During the tenure of this project, we demonstrated that bone forming cells communicate via gap junctions formed primarily by connexin43 (Cx43)...
  8. Membrane skeleton regulation of cell shape and interactions in lens development
    Velia M Fowler; Fiscal Year: 2013
    ..provided by applicant): The transparency of the vertebrate lens depends on assembly of connexins into gap junctions that are required for lens ionic homeostasis and metabolite circulation...
  9. Internalization of gap junctions as a regulatory mechanism of direct GJIC
    Matthias M Falk; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Communication between adjacent cells through gap junctions (GJs) occurs in nearly every tissue and is fundamental to coordinated cell behavior during development, differentiation, and tissue ..
  10. Probing localiztion, interactions, and effector properties of Shigella lpaD
    NICHOLAS EUGENE DICKENSON; Fiscal Year: 2010
    ..flexneri, but it is injected into the host cell cytoplasm where it may be localized to gap junctions. Because IpaD may have a role in post-invasion events (i.e...
  11. Biology of desmosome-gap junctions in the testis
    Dolores D Mruk; Fiscal Year: 2010
    ..In the testis, morphological studies in the 1970s reported the presence of desmosome-gap junctions (also known as desmosome-like junctions) between adjacent Sertoli cells at the blood-testis barrier, as well ..
  12. GENETIC STUDIES OF ODDD
    Ethylin Wang Jabs; Fiscal Year: 2010
    ..are known to form connexons that align as pairs in apposing cell membranes to form specialized intercellular gap junctions. These channels confer distinct physiologic properties by providing an intercellular passage for ions and ..
  13. Molecular mechanisms of electrical synapse formation in vivo
    Adam C Miller; Fiscal Year: 2013
    ..phase of the project I will utilize the acquired skills to illuminate the molecular mechanisms that build gap junctions at the electrical synapse...
  14. Characterization of the role of gap junction proteins in ER stress and obesity
    Amir Tirosh; Fiscal Year: 2013
    ..b>Gap junctions facilitate direct cytoplasmic communication between neighboring cells, allowing the transfer of small-..
  15. Actions of Parathyroid Hormone (PTH)/PTH Related-Peptide Receptor in Osteocytes
    PAOLA DIVIETI PAJEVIC; Fiscal Year: 2013
    ..the mineralized matrix of bone and communicate with one another and with osteoblasts and osteoclasts via gap junctions located at the ends of long cytoplasmic processes that course through tunnels (cannalicula) in the bone...
  16. Use of a Fragile X premutation knock-in mouse to study FXPOI
    Joshua Johnson; Fiscal Year: 2013
    ..Aim 2 focuses on the abnormal FMRP, ubiquitin, and gap junctions as well as the abnormalities in zona pellucida composition to attempt to uncover the mechanism(s) responsible ..
  17. Gap junctions in Vascular Smooth Muscle: growth control
    Janis M Burt; Fiscal Year: 2013
    ..the molecular mechanisms that govern the permeability and growth suppressive properties of vascular cell gap junctions and 2) to develop from that knowledge the rationale for gene therapies that target connexin expression in ..
  18. Gap Junction Trafficking to and within the Plasma Membrane
    ROBIN MARK SHAW; Fiscal Year: 2013
    ..disease includes loss of cardiomyocyte electrical coupling through altered localization and modulation of gap junctions. The precise molecular mechanisms underlying altered coupling remain elusive...
  19. Regulation of conducted hyperpolarization in microvascular endothelial cell tubes
    ERIK JOSEF BEHRINGER; Fiscal Year: 2012
    ..Endothelial cells (ECs) provide the predominant cellular pathway for conducted hyperpolarization (CHP) through gap junctions (GJs) along arterioles and feed arteries...
  20. MECHANISMS OF GATING AND PERMEATION IN GAP JUNCTIONS
    VYTAUTAS K VERSELIS; Fiscal Year: 2013
    ..These studies should also shed light on a growing list of disorders ascribed to hemichannel dysfunction that includes atherosclerosis, stroke, neuropathy and congenital cataractogenesis and should lead to strategies for treatment. ..
  21. Cyclic nucleotide permeability of connexin mutants related to genetic disease
    Virginijus Valiunas; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Gap junctions provide a direct intercellular pathway for cell-to-cell signaling and impulse conduction and play an important role in normal physiology...
  22. Connexin 43 Hemichannels and Signaling In Bone
    LILIAN IRENE PLOTKIN; Fiscal Year: 2012
    ..hormonal and mechanical stimuli in osteoblasts and osteocytes, via its involvement in hemichannels or gap junctions or its scaffolding properties...
  23. Connexin Distribution in Physiological Versus Pathological Cardiac Hypertrophy
    Michael R Zile; Fiscal Year: 2013
    ..and the attendant densifica- tion of the microtubule network, inhibit the normal transport of Cx43 to gap junctions as well as Cx43-depen- dent electrophysiological function...
  24. Cx26 mutations in syndromic deafness linked to skin disease
    Thomas W White; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Connexins are the subunit proteins of gap junctions, which allow the exchange of ions, second messengers and small metabolites between adjacent cells through intercellular channels...
  25. Mechanisms for Stem Cell Differentiation into Cardiac Myocytes
    Margaret Loewy Kirby; Fiscal Year: 2010
    ..More specifically, shared cytosolic conduits, gap junctions, between adult-derived stem cells and adjacent contracting cardiomyocytes provide the route by which a signal ..
  26. Development of a novel connexin-based peptide for the treatment of corneal injury
    GAUTAM SUDHIR GHATNEKAR; Fiscal Year: 2012
    ..ACT1 works by stabilizing gap junctions and maintaining intercellular communication between cells that is essential for wound repair or injury ..
  27. STRUCTURAL ANALYSIS OF GAP JUNCTION TRAFFICKING
    Gina E Sosinsky; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Gap junctions create direct cell-cell communication in most cell types...
  28. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul D Lampe; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Gap junctions are specialized matched membrane domains that contain channels that allow exchange of small molecules including ions, metabolites, and second messengers (e.g...
  29. Effects of Neuroinflammation on Gap Junction Communication in Glia
    TAMMY L KIELIAN; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): Gap junctions represent direct intercellular conduits between contacting cells that permit the passage of small molecules (>1 kDa) including ions, metabolic precursors, and second messengers...
  30. SIGNAL TRANSDUCTION AT FERTILIZATION
    Laurinda A Jaffe; Fiscal Year: 2012
    ..Recent findings, which establish that gap junctions between the somatic cells of the follicle close rapidly in response to LH, form the basis for the 3 aims of ..
  31. Caspase-3 Maintains Uterine Quiescence in a PR and NF-Kappa B Dependent Manner
    Jennifer C Condon; Fiscal Year: 2013
    ..The priming of the pregnant uterus for contraction is associated with increased oxytocin receptors, increased gap junctions and cervical ripening...
  32. MICROVASCULAR TRANSPORT IN THE RENAL MEDULLA
    Thomas L Pallone; Fiscal Year: 2013
    ..endothelium have shown that it is an electrical syncytium in which cells strongly couple to one another via gap junctions. External K+ is a strong regulator gap junction conductance...
  33. Calcium Signals Within Membrane Nanotubes
    Ian F Smith; Fiscal Year: 2013
    ..for the coordinated functioning of multi-cellular organisms, and cells have long been known to employ gap junctions and synapses to communicate with their neighbors...
  34. Connexin acetylation: Impact on gap junction function and HDAC inhibitor respons
    JEANNETTE CHLOE BULINSKI; Fiscal Year: 2010
    ..b>Gap junctions are channels formed from connexin subunits that permit the passive transfer of low molecular mass hydrophilic ..
  35. Connexin 43 gap junction dynamics in the diabetic heart
    Joseph A Palatinus; Fiscal Year: 2013
    ..2B. Determine if hyperglycemia induced remodeling of Cx43 gap junctions are normalized by a membrane permanent peptide (ACT-1) designed to inhibit the interaction of ZO-1 with Cx43 ..
  36. Pharmacology of Connexin Channels: Structure-Activity Studies.
    Miduturu Srinivas; Fiscal Year: 2010
    DESCRIPTION (provided by applicant): The multi-gene family of proteins called connexins form intercellular gap junctions that directly mediate signaling between adjacent cells...
  37. Electro-mechanical Resynchronization Therapy For Arrhythmogenic Right Ventricular
    Harikrishna Tandri; Fiscal Year: 2012
    ..delay in the RV, namely the localization and expression of the key conduction protein connexin-43 at the gap junctions, and its relation to electrical propagation;and 3)...
  38. Endoplasmic reticulum stress in diabetic brain
    Gerald A Dienel; Fiscal Year: 2012
    ..The anticipated results will lead to a better understanding of nutritional and signaling roles of gap junctions in astrocytes and other brain cells and how these functions are disrupted by hyperglycemia and experimental ..
  39. The glymphatic system, a new concept in glia biology
    Maiken Nedergaard; Fiscal Year: 2013
    ..Aim 3 proposes that induced knock-out of either astrocytic AQP4 water channels or gap junctions (Cx43/Cx30) will slow parenchymal convective fluid flow and globally suppress tracer clearance...
  40. STRUCTURE AND FUNCTION OF LENS CHANNELS
    JAMES EWBANK HALL; Fiscal Year: 2013
    ..Lens cells have glucose transporters, chloride channels, sodium and potassium channels, gap junctions, and many other standard transport proteins in common with the cells of other organs...
  41. A gap junction-independent role for Connexin43 in cardiac electrophysiology
    Mario Delmar; Fiscal Year: 2013
    ..Since its inception (in 1999), we focused on the mechanisms that regulate gap junctions formed by Connexin43 (Cx43)...
  42. Pancreatic Islets Dynamics Regulating Glucagon Secretion
    David W Piston; Fiscal Year: 2013
    ..This effect parallels that seen in islets lacking [unreadable]-cell gap junctions, where insulin secretion can be inhibited by juxtacrine communication mediated by ephrinA-EphA signaling even ..
  43. Mechanisms of coronary endothelial dysfunction in diabetes
    Ayako Makino; Fiscal Year: 2011
    ..b>Gap junctions, formed by connexins (Cxs), provide important pathways for the cell-cell communication and Cx40 is known to be ..
  44. Electrical Synapses and Network Signaling
    Gianmaria Maccaferri; Fiscal Year: 2010
    ..b>Gap junctions shape GABAergic network activity and integration by synchronizing firing and boosting the propagation of ..
  45. MicroRNA as mediators of angiogenesis &ischemic myocardial repair
    Meifeng Xu; Fiscal Year: 2013
    ..Hypothesis 2: miRs in transplanted MSC transfer to neighboring host cells or the extracellular environment via gap junctions and microvesicles (MVs)...
  46. Mechanisms of Gap Junction Regulation
    Paul L Sorgen; Fiscal Year: 2013
    ..b>Gap junctions mediate vitally important processes such as electrical impulse propagation, regulation of cell growth, and ..
  47. Perisomatic Inhibitory Network Dysfunction in Neurological Disease
    Vijayalakshmi Santhakumar; Fiscal Year: 2013
    ..and dynamically regulated during seizures will help evaluate whether pharmacological manipulation of gap junctions and GABA receptors would be effective in treating epilepsy...
  48. GAP JUNCTIONS AND IONIC CURRENTS IN DEVELOPING HEART
    Jenny J Yang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Gap junctions provide for the homeostasis of nearly every tissue by metabolically, chemically, and electrically coupling the cells into a functional syncytium...
  49. Connexin-Mediated Hemichannel Activity in Experimental Periodontitis
    Karen F Ross; Fiscal Year: 2012
    ..this project is to characterize cell-to-cell communication in oral tissues through connexin hemichannels and gap junctions in order to design new treatments for infection of mucosal tissues...
  50. Cadherin Mediated Cell-Cell Interactions in the Bone Microenvironment
    Roberto Civitelli; Fiscal Year: 2013
    ..Cell-cell adhesion is also a pre-requisite for assembly of gap junctions and intercellular communication...
  51. Structure and Function of Retinal Ganglion Cell Gap Junctions
    Bela Volgyi; Fiscal Year: 2011
    ..has dramatically changed over the last decade as converging evidence indicates that electrical synapses via gap junctions form a second and significant mode of interneuronal communication...
  52. Lens intercellular communication connexins and cataract
    Thomas W White; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Connexins are the subunit proteins of gap junctions, which allow the exchange of ions, second messengers and small metabolites between adjacent cells through intercellular channels...
  53. CELL JUNCTIONS AND CELL MEMBRANES IN THE LENS
    WOO KUEN K LO; Fiscal Year: 2011
    ..in trafficking of cholesterol will be examined for the transformation of cholesterol-rich to cholesterol-free gap junctions during junction assembly, maturation, and aging using our innovative methodology combining filipin ..
  54. Positional Cloning and Candidate Gene Approach to Familial Atrial Fibrilation
    Dawood Darbar; Fiscal Year: 2012
    ..In addition, mutations in genes encoding cardiac potassium channels and gap junctions have been reported in isolated cases and small kindreds...
  55. Structure and Dynamics of Gap Junction Channels
    MARK JAY YEAGER; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Gap junctions are specialized regions of cell-to-cell contact in which hexameric oligomers, called connexons, dock end-to-end across a narrow extracellular gap, allowing the intercellular exchange of ..
  56. INTERCELLULAR COMMUNICATION IN THE LENS
    Lisa Ebihara; Fiscal Year: 2013
    ..The central hypothesis that will be tested is that both connexin gap junctions and hemichannels play an important role in maintaining lens transparency. There are two specific aims: Aim 1...
  57. Mechanisms enhancing functional coupling between native and embryonic stem cell d
    Karen Maass; Fiscal Year: 2013
    ..While experiments proposed in this study will elucidate ways to enhance the formation of gap junctions in stem cell derived cardiomyocytes, the mechanisms identified might very well also be applicable and ..
  58. Cellular Cardiomyoplasty for Chronic Heart Failure
    Joshua M Hare; Fiscal Year: 2013
    ..of autologous CSCs and MSCs, 2) assess the impact of cell-cell communication by connexin 43 (Cx43)-mediated gap junctions on cardiac regeneration, and 3) assess the cell- cell interaction and efficacy of pharmacologically and/or ..
  59. INTERCELLULAR COMMUNICATION AND IMPULSE PROPAGATION
    JOSE S JALIFE; Fiscal Year: 2010
    ..Project 3 focuses on the characterization of the function and regulation of cardiac gap junctions in a mouse line where the Cx43 molecule has been replaced with a truncation mutant lacking the carboxyl ..
  60. SiRNA therapeutics: Gap junction delivery in vitro and in vivo
    Richard T Mathias; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The cellular delivery of siRNA via gap junctions represents a unique and potentially clinically important delivery system...
  61. CENTRAL RHYTHMOGENESIS AND BEHAVIOR
    John P Welsh; Fiscal Year: 2013
    This project will focus on the hypothesis that N-methyl-D-aspartate (NMDA) glutamate receptors and neuronal gap junctions act concertedly to determine the spatio-temporal dynamics of activity within electrically coupled networks in ..
  62. Cx43 Hemichannels: Gating, Modification and Function
    Michael V L Bennett; Fiscal Year: 2011
    DESCRIPTION (provided by applicant): Gap junctions are formed of two hemichannels (or connexons), one from each of the apposed cells...
  63. Function and Expression of Connexins in the pre-Botzinger Complex
    JONATHAN KELTY; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): Gap junctions are pores that span the membranes of adjacent cells, and as such provide a means of electrical and cytoplasmic continuity between those cells...
  64. GAP JUNCTIONS AND LUTEAL FUNCTION
    ANNA GRAZUL BILSKA; Fiscal Year: 1999
    ..Structural and functional gap junctions have been demonstrated in luteal tissues of several species, and probably play a critical role in ..
  65. NEURONAL DEVELOPMENT INTERACTION AND ORGANIZATION
    Michael Bennett; Fiscal Year: 2000
    ..Communication by gap junctions can be reduced by antisense DNAs and by gene knockout through homologous recombination, and it can be ..
  66. Connexins in Ischemia-Induced Neuronal Death
    Michael Bennett; Fiscal Year: 2007
    ..The proposed research aims to study the role of gap junctions during the several days of "maturation" of neuronal injury after global ischemia...
  67. Communication and Chemoprevention of Prostate Cancer
    Parmender Mehta; Fiscal Year: 2009
    ..b>Gap junctions, which are formed of proteins called connexins, are a special class of cell junctions that provide a direct ..
  68. BILE SECRETION AND INTRACELLULAR CA2+ SIGNALS
    Michael Nathanson; Fiscal Year: 1999
    ..These Ca2+ waves then spread from cell to cell across gap junctions, in a fashion that depends on the type(s) of gap junctions expressed...
  69. Local Signals and Macromolecular Architecture in Heart
    Terry Rogers; Fiscal Year: 2006
    ..association between Ca2+ signaling elements in t-tubules and the conduction macromolecular complexes at gap junctions. These crucial molecules are locally organized and controlled by signaling cascades that interact with the ..
  70. Gap Junctions in Placental Development
    Stephen Lye; Fiscal Year: 2004
    ..In other tissues, intercellular communication via gap junctions is known to modulate these processes, though there is no information as to whether this occurs in the placenta...
  71. The Role of Ductin in Embryonic Morphogenesis
    Michael Levin; Fiscal Year: 2007
    ..Direct exchange of chemical and electrical signals through gap junctions between cells controls migration, proliferation, and differentiation as a crucial part of normal physiology, ..
  72. FUNCTION OF ELECTRICAL COUPLING IN EMBRYONIC TISSUE
    Michael Bennett; Fiscal Year: 1991
    ..b>Gap junctions in the ascidians show voltage dependence and the blastomeres exhibit two stable resting potentials ..
  73. INNER EAR GAP JUNCTIONS FOR HEARING
    Hong Bo Zhao; Fiscal Year: 2002
    ..Disturbances of inner ear gap junctions are known to be associated with a high incidence of non-syndromic deafness...
  74. The Role of Gap Junctions in Peripheral Odor Coding
    Chunbo Zhang; Fiscal Year: 2004
    b>Gap junctions represent an important mode of intercellular communication in the nervous system. Gap junctions allow exchange of small bioactive substances, including second messengers, between coupled cells...
  75. The Role of Connexin32 in the Pathogenesis of CMTX
    Steven Scherer; Fiscal Year: 2006
    ..b>Gap junctions allow the diffusion of ions and small molecules, typically with a molecular mass less than 1000 Da...
  76. SCOR IN SUDDEN CARDIAC DEATH
    Douglas Zipes; Fiscal Year: 1999
    ..remodeling alters the number, distribution or function of several important cardiac structures, including gap junctions and autonomic innervation patterns, that are directly and indirectly responsible for normal cell-to-cell ..
  77. GAP JUNCTIONS IN OLFACTION
    VINCENT DIONNE; Fiscal Year: 1999
    This study is directed at identifying and characterizing the physiological roles of gap junctions in the olfactory epithelium...